MD04G1200000.v1.1

Heparan-alpha-glucosaminide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003888
Physical Location & Seq
Reverse (-)
28680671 .. 28684559
3889 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1200000.v1.1.491

Sequence Viewer

Length: 1464 bp
ATGCAATCATCAGTCTATATCAGCTCATCACCAAAGACAGTTGAACACGAACCCACAAAAATGGCTGAAATCAAAGCAGATACGAGCCACCACCAGAGTCTAATCGTGTCGGAACCCGATGTTTCCGACCCCAAGCCATCCAAATCGAAGCGCCTCGCTTCCCTCGACATCTTTCGTGGCCTCACCGTCTCGTTGATGATTCTGGTTGACGATGCGGGTGGAGAGTGGCCAGTGATCGGTCATGCGCCATGGAACGGCTGCAATCTTGCAGACTTTGTGATGCCCTTCTTCCTCTTCATTGTGGGCATGTCCATTGCTCTTTCTCTCAAGAGAATACCGGACCAATTTGTGGCTGTCAAGAAGGTGATTCTTAGAACTCTGAAGCTACTGTTTTGGGGTCTCCTATTACAAGGAGGTTACTCACATGCTCCTGACAAACTAACATATGGTGTCGACATGAAAGAATTAAGGTGGTGCGGTATTCTCCAGAGAATTGCTCTTGCATATCTGGTTGTGGCACTGATTGAAATTGTATCGAGAGGTGCAGAAACCAAAGATATGGCACCCGGCACATTCTCCATATTCAAGTTGTACTATTGGCACTGGCTTGTAGCTGGATGTGTTCTCGTTATTTACTTTGCTGTGATTTATGGGACATATGTTCCTGATTGGCAATTTACTGTCCAGGACAGAGAGAGAACCGATTATGGGAAAAGTTACACCGTAGCTTGTGGCGTGAGAGGAAAACTAGATCCTCCGTGTAATGCAGTAGGATATATTGACAGAGAAGTGTTGGGAATCAGTCACATGTATCAGCGTCCGGCTTGGAAACGATCCAAAGCTTGCACTGAGAATTCCCCCTATGCAGGACCATTCCGAAATGATGCCCCATCATGGTGCCGTGCTCCGTTTGAACCTGAAGGAATTGTAAGCTCTATATCTGCAATTCTCTCCGCGATTATTGGAGTGCATTTCGGACATGTGCTTATACATATGCAGGGTCATCCAGCCAGACTGAAGCATTGGGTTCCGACAGGCTGTGCTCTCCTTGCCCTGGGAATTATTCTCCATTTCAGTCATGCAATTCCTTCAAATAAACAGCTGTACACATTTAGCTACGTATGCATAACATCGGGAGTGGCAGCCTTGGTGTTTTCAGCCTTCTACCTTATGGTTGATATCTGGAGCATAAGGTACCTGTTCCTGCCGCTGGAATGGATCGGCATGAATGCTATGCTCGTCTATGTCATGGCAGCTGAAGGCATCCTCGCAGGATTCGTTAACGGGTGGTATTACAAGGACCCCCATAACACGCTTGTGTACTGGATTCAAAAGCACGTCTTCGTCGAGGTTTGGCATTCGAGGAGAGTAGGCATTCTACTCTACGTCCTCTTTGCAGAGATCTTGTTTTGGGGCATTGTCGCCGGCGTTTTGCATCGGCTGGGCATATATTGGAAGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624 DUF5009

Protein Analysis

488

Amino Acids

54.87

Weight (kDa)

8.78

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 1194
AccB1I GGYRCC 3 cut(s) 562, 897, 1194
AccB7I CCANNNNNTGG 1 cut(s) 349
AccI GTMKAC 1 cut(s) 453
AccII CGCG 1 cut(s) 956
AciI CCGC 4 cut(s) 215, 477, 954, 1208
AclWI GGATC 3 cut(s) 746, 828, 1226
AcoI YGGCCR 1 cut(s) 227
AcsI RAATTY 1 cut(s) 853
AcuI CTGAAG 4 cut(s) 401, 939, 1037, 1278
AfaI GTAC 4 cut(s) 593, 1106, 1196, 1322
AfiI CCNNNNNNNGG 8 cut(s) 236, 254, 349, 708, 866, 894, 1054, 1210
AflIII ACRYGT 2 cut(s) 807, 979
AgsI TTSAA 6 cut(s) 44, 527, 586, 914, 1092, 1331
AjiI CACGTC 1 cut(s) 1339
AjnI CCWGG 2 cut(s) 684, 1053
AleI CACNNNNGTG 1 cut(s) 1316
Alw21I GWGCWC 2 cut(s) 907, 1045
Alw26I GTCTC 2 cut(s) 193, 404
AlwI GGATC 3 cut(s) 746, 828, 1226
AoxI GGCC 2 cut(s) 178, 227
ApeKI GCWGC 3 cut(s) 258, 1142, 1253
ApoI RAATTY 1 cut(s) 853
Asp718I GGTACC 1 cut(s) 1194
AspLEI GCGC 2 cut(s) 153, 247
AspS9I GGNCC 3 cut(s) 340, 869, 1300
AsuC2I CCSGG 1 cut(s) 567
AsuHPI GGTGA 3 cut(s) 21, 175, 376
AvaII GGWCC 3 cut(s) 340, 869, 1300
BalI TGGCCA 1 cut(s) 229
BanI GGYRCC 3 cut(s) 562, 897, 1194
BbsI GAAGAC 1 cut(s) 1333
Bbv12I GWGCWC 2 cut(s) 907, 1045
BbvI GCAGC 3 cut(s) 245, 1154, 1265
BccI CCATC 2 cut(s) 145, 898
BceAI ACGGC 2 cut(s) 271, 885
BciT130I CCWGG 2 cut(s) 686, 1055
BcnI CCSGG 1 cut(s) 567
BcoDI GTCTC 2 cut(s) 193, 404
BfaI CTAG 1 cut(s) 749
BfoI RGCGCY 1 cut(s) 154
BglII AGATCT 1 cut(s) 1401
BisI GCNGC 4 cut(s) 259, 1143, 1208, 1254
BlsI GCNGC 4 cut(s) 260, 1144, 1209, 1255
Bme1390I CCNGG 3 cut(s) 567, 686, 1055
Bme18I GGWCC 3 cut(s) 340, 869, 1300
BmgBI CACGTC 1 cut(s) 1339
BmgT120I GGNCC 3 cut(s) 340, 869, 1300
BmiI GGNNCC 6 cut(s) 114, 564, 899, 1029, 1196, 1302
BmrFI CCNGG 3 cut(s) 567, 686, 1055
BmsI GCATC 5 cut(s) 202, 270, 874, 1272, 1444
BpiI GAAGAC 1 cut(s) 1333
BplI GAGNNNNNCTC 2 cut(s) 481, 513
BpmI CTGGAG 2 cut(s) 470, 1204
BpuEI CTTGAG 1 cut(s) 311
BpuMI CCSGG 1 cut(s) 567
BsaAI YACGTR 1 cut(s) 1120
BsaI GGTCTC 1 cut(s) 404
BsaJI CCNNGG 4 cut(s) 248, 1053, 1054, 1146
BsaWI WCCGGW 1 cut(s) 337
Bsc4I CCNNNNNNNGG 8 cut(s) 236, 254, 349, 708, 866, 894, 1054, 1210
Bse118I RCCGGY 1 cut(s) 1424
Bse1I ACTGG 3 cut(s) 230, 608, 1328
Bse3DI GCAATG 1 cut(s) 312
BseBI CCWGG 2 cut(s) 686, 1055
BseDI CCNNGG 4 cut(s) 248, 1053, 1054, 1146
BseGI GGATG 4 cut(s) 137, 623, 1003, 1263
BseLI CCNNNNNNNGG 8 cut(s) 236, 254, 349, 708, 866, 894, 1054, 1210
BseMI GCAATG 1 cut(s) 312
BseMII CTCAG 1 cut(s) 840
BseNI ACTGG 3 cut(s) 230, 608, 1328
BseRI GAGGAG 1 cut(s) 1378
BseXI GCAGC 3 cut(s) 245, 1154, 1265
BseYI CCCAGC 1 cut(s) 1441
BsgI GTGCAG 1 cut(s) 564
Bsh1236I CGCG 1 cut(s) 956
BshFI GGCC 2 cut(s) 180, 229
BshNI GGYRCC 3 cut(s) 562, 897, 1194
BsiHKAI GWGCWC 2 cut(s) 907, 1045
BsiSI CCGG 4 cut(s) 338, 567, 821, 1425
BslFI GGGAC 1 cut(s) 667
BslI CCNNNNNNNGG 8 cut(s) 236, 254, 349, 708, 866, 894, 1054, 1210
BsmAI GTCTC 2 cut(s) 193, 404
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 667
BsmI GAATGC 3 cut(s) 1234, 1357, 1374
BsnI GGCC 2 cut(s) 180, 229
Bso31I GGTCTC 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 907, 1045
Bsp1407I TGTACA 1 cut(s) 1104
Bsp143I GATC 5 cut(s) 234, 751, 833, 1218, 1401
Bsp19I CCATGG 1 cut(s) 248
BspACI CCGC 4 cut(s) 215, 477, 954, 1208
BspANI GGCC 2 cut(s) 180, 229
BspCNI CTCAG 1 cut(s) 841
BspFNI CGCG 1 cut(s) 956
BspLI GGNNCC 6 cut(s) 114, 564, 899, 1029, 1196, 1302
BspPI GGATC 3 cut(s) 746, 828, 1226
BspT107I GGYRCC 3 cut(s) 562, 897, 1194
BspTNI GGTCTC 1 cut(s) 404
BsrDI GCAATG 1 cut(s) 312
BsrFI RCCGGY 1 cut(s) 1424
BsrGI TGTACA 1 cut(s) 1104
BsrI ACTGG 3 cut(s) 230, 608, 1328
BssAI RCCGGY 1 cut(s) 1424
BssECI CCNNGG 4 cut(s) 248, 1053, 1054, 1146
BssMI GATC 5 cut(s) 234, 751, 833, 1218, 1401
BssT1I CCWWGG 2 cut(s) 248, 1146
Bst2UI CCWGG 2 cut(s) 686, 1055
Bst4CI ACNGT 5 cut(s) 40, 187, 390, 682, 724
Bst6I CTCTTC 1 cut(s) 299
BstAUI TGTACA 1 cut(s) 1104
BstBAI YACGTR 1 cut(s) 1120
BstC8I GCNNGC 2 cut(s) 844, 1426
BstDEI CTNAG 2 cut(s) 371, 849
BstDSI CCRYGG 1 cut(s) 248
BstF5I GGATG 4 cut(s) 137, 623, 1003, 1263
BstFNI CGCG 1 cut(s) 956
BstH2I RGCGCY 1 cut(s) 154
BstHHI GCGC 2 cut(s) 153, 247
BstKTI GATC 5 cut(s) 237, 754, 836, 1221, 1404
BstMAI GTCTC 2 cut(s) 193, 404
BstMBI GATC 5 cut(s) 234, 751, 833, 1218, 1401
BstMWI GCNNNNNNNGC 2 cut(s) 1049, 1122
BstNI CCWGG 2 cut(s) 686, 1055
BstNSI RCATGY 4 cut(s) 310, 428, 811, 983
BstSCI CCNGG 3 cut(s) 565, 684, 1053
BstSNI TACGTA 1 cut(s) 1120
BstUI CGCG 1 cut(s) 956
BstV1I GCAGC 3 cut(s) 245, 1154, 1265
BstV2I GAAGAC 1 cut(s) 1333
BstX2I RGATCY 2 cut(s) 751, 1401
BstXI CCANNNNNNTGG 2 cut(s) 61, 559
BstYI RGATCY 2 cut(s) 751, 1401
BsuRI GGCC 2 cut(s) 180, 229
BtgI CCRYGG 1 cut(s) 248
BtrI CACGTC 1 cut(s) 1339
BtsCI GGATG 4 cut(s) 137, 623, 1003, 1263
BtsIMutI CAGTG 4 cut(s) 237, 518, 601, 846
Cac8I GCNNGC 2 cut(s) 844, 1426
CfoI GCGC 2 cut(s) 153, 247
Cfr10I RCCGGY 1 cut(s) 1424
Cfr13I GGNCC 3 cut(s) 340, 869, 1300
CseI GACGC 1 cut(s) 806
Csp6I GTAC 4 cut(s) 592, 1105, 1195, 1321
CviQI GTAC 4 cut(s) 592, 1105, 1195, 1321
DdeI CTNAG 2 cut(s) 371, 849
DpnI GATC 5 cut(s) 236, 753, 835, 1220, 1403
DpnII GATC 5 cut(s) 234, 751, 833, 1218, 1401
EaeI YGGCCR 1 cut(s) 227
Eam1104I CTCTTC 1 cut(s) 299
EarI CTCTTC 1 cut(s) 299
Eco105I TACGTA 1 cut(s) 1120
Eco130I CCWWGG 2 cut(s) 248, 1146
Eco31I GGTCTC 1 cut(s) 404
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 3 cut(s) 340, 869, 1300
Eco57I CTGAAG 4 cut(s) 401, 939, 1037, 1278
EcoO109I RGGNCCY 1 cut(s) 1300
EcoRI GAATTC 1 cut(s) 853
EcoRII CCWGG 2 cut(s) 684, 1053
EcoRV GATATC 1 cut(s) 1180
EcoT14I CCWWGG 2 cut(s) 248, 1146
EcoT22I ATGCAT 1 cut(s) 1127
ErhI CCWWGG 2 cut(s) 248, 1146
Esp3I CGTCTC 1 cut(s) 193
FalI AAGNNNNNCTT 2 cut(s) 1325, 1357
FaqI GGGAC 1 cut(s) 667
FauI CCCGC 1 cut(s) 208
FauNDI CATATG 3 cut(s) 445, 658, 993
FblI GTMKAC 1 cut(s) 453
Fnu4HI GCNGC 4 cut(s) 259, 1143, 1208, 1254
FokI GGATG 4 cut(s) 124, 630, 990, 1250
Fsp4HI GCNGC 4 cut(s) 259, 1143, 1208, 1254
FspBI CTAG 1 cut(s) 749
GlaI GCGC 2 cut(s) 152, 246
GluI GCNGC 4 cut(s) 259, 1143, 1208, 1254
GsaI CCCAGC 1 cut(s) 1445
GsuI CTGGAG 2 cut(s) 470, 1204
HaeII RGCGCY 1 cut(s) 154
HaeIII GGCC 2 cut(s) 180, 229
HapII CCGG 4 cut(s) 338, 567, 821, 1425
HgaI GACGC 1 cut(s) 806
HhaI GCGC 2 cut(s) 153, 247
Hin6I GCGC 2 cut(s) 151, 245
HinP1I GCGC 2 cut(s) 151, 245
HincII GTYRAC 3 cut(s) 208, 454, 1282
HindII GTYRAC 3 cut(s) 208, 454, 1282
HindIII AAGCTT 2 cut(s) 840, 1457
HinfI GANTC 6 cut(s) 97, 199, 367, 798, 1275, 1327
HpaI GTTAAC 1 cut(s) 1282
HpaII CCGG 4 cut(s) 338, 567, 821, 1425
HphI GGTGA 3 cut(s) 21, 175, 376
Hpy166II GTNNAC 5 cut(s) 208, 454, 1107, 1282, 1321
Hpy188I TCNGA 6 cut(s) 112, 127, 381, 878, 977, 1032
Hpy188III TCNNGA 8 cut(s) 328, 358, 431, 487, 537, 665, 1134, 1183
Hpy8I GTNNAC 5 cut(s) 208, 454, 1107, 1282, 1321
Hpy99I CGWCG 1 cut(s) 1349
HpyAV CCTTC 6 cut(s) 295, 355, 914, 1098, 1171, 1253
HpyCH4III ACNGT 5 cut(s) 40, 187, 390, 682, 724
HpyCH4IV ACGT 3 cut(s) 1119, 1338, 1386
HpyF10VI GCNNNNNNNGC 2 cut(s) 1049, 1122
HpyF3I CTNAG 2 cut(s) 371, 849
HpySE526I ACGT 3 cut(s) 1119, 1338, 1386
HspAI GCGC 2 cut(s) 151, 245
KpnI GGTACC 1 cut(s) 1198
KroI GCCGGC 1 cut(s) 1424
KroNI GCCGGC 1 cut(s) 1426
KspAI GTTAAC 1 cut(s) 1282
Kzo9I GATC 5 cut(s) 234, 751, 833, 1218, 1401
LmnI GCTCC 3 cut(s) 433, 910, 1185
Lsp1109I GCAGC 3 cut(s) 245, 1154, 1265
LweI GCATC 5 cut(s) 202, 270, 874, 1272, 1444
MaeI CTAG 1 cut(s) 749
MaeII ACGT 3 cut(s) 1119, 1338, 1386
MaeIII GTNAC 3 cut(s) 416, 716, 803
MalI GATC 5 cut(s) 236, 753, 835, 1220, 1403
MboI GATC 5 cut(s) 234, 751, 833, 1218, 1401
MboII GAAGA 3 cut(s) 280, 286, 1333
MflI RGATCY 2 cut(s) 751, 1401
MhlI GDGCHC 2 cut(s) 907, 1045
MlsI TGGCCA 1 cut(s) 229
MluNI TGGCCA 1 cut(s) 229
MlyI GAGTC 1 cut(s) 106
MmeI TCCRAC 3 cut(s) 90, 150, 1055
Mox20I TGGCCA 1 cut(s) 229
Mph1103I ATGCAT 1 cut(s) 1127
MreI CGCCGGCG 1 cut(s) 1424
MroNI GCCGGC 1 cut(s) 1424
MscI TGGCCA 1 cut(s) 229
MseI TTAA 2 cut(s) 467, 1281
MslI CAYNNNNRTG 3 cut(s) 59, 895, 1316
Msp20I TGGCCA 1 cut(s) 229
MspA1I CMGCKG 3 cut(s) 1102, 1210, 1256
MspI CCGG 4 cut(s) 338, 567, 821, 1425
MspR9I CCNGG 3 cut(s) 567, 686, 1055
Mva1269I GAATGC 3 cut(s) 1234, 1357, 1374
MvaI CCWGG 2 cut(s) 686, 1055
MvnI CGCG 1 cut(s) 956
MwoI GCNNNNNNNGC 2 cut(s) 1049, 1122
NaeI GCCGGC 1 cut(s) 1426
NciI CCSGG 1 cut(s) 567
NcoI CCATGG 1 cut(s) 248
NdeI CATATG 3 cut(s) 445, 658, 993
NdeII GATC 5 cut(s) 234, 751, 833, 1218, 1401
NgoMIV GCCGGC 1 cut(s) 1424
NlaIV GGNNCC 6 cut(s) 114, 564, 899, 1029, 1196, 1302
NmuCI GTSAC 1 cut(s) 803
NsiI ATGCAT 1 cut(s) 1127
NspI RCATGY 4 cut(s) 310, 428, 811, 983
OliI CACNNNNGTG 1 cut(s) 1316
PasI CCCWGGG 1 cut(s) 1054
PciI ACATGT 2 cut(s) 807, 979
PcsI WCGNNNNNNNCGW 3 cut(s) 162, 1275, 1344
PctI GAATGC 3 cut(s) 1234, 1357, 1374
PdiI GCCGGC 1 cut(s) 1426
PfeI GAWTC 5 cut(s) 199, 367, 798, 1275, 1327
PflMI CCANNNNNTGG 1 cut(s) 349
PfoI TCCNGGA 1 cut(s) 684
PkrI GCNGC 4 cut(s) 260, 1144, 1209, 1255
PleI GAGTC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 105
Ppu21I YACGTR 1 cut(s) 1120
PpuMI RGGWCCY 1 cut(s) 1300
PscI ACATGT 2 cut(s) 807, 979
Psp5II RGGWCCY 1 cut(s) 1300
Psp6I CCWGG 2 cut(s) 684, 1053
PspFI CCCAGC 1 cut(s) 1441
PspGI CCWGG 2 cut(s) 684, 1053
PspN4I GGNNCC 6 cut(s) 114, 564, 899, 1029, 1196, 1302
PspPI GGNCC 3 cut(s) 340, 869, 1300
PspPPI RGGWCCY 1 cut(s) 1300
PsuI RGATCY 2 cut(s) 751, 1401
PvuII CAGCTG 2 cut(s) 1102, 1256
RsaI GTAC 4 cut(s) 593, 1106, 1196, 1322
RsaNI GTAC 4 cut(s) 592, 1105, 1195, 1321
RseI CAYNNNNRTG 3 cut(s) 59, 895, 1316
SalI GTCGAC 1 cut(s) 452
SaqAI TTAA 2 cut(s) 467, 1281
SatI GCNGC 4 cut(s) 259, 1143, 1208, 1254
Sau3AI GATC 5 cut(s) 234, 751, 833, 1218, 1401
Sau96I GGNCC 3 cut(s) 340, 869, 1300
SchI GAGTC 1 cut(s) 106
ScrFI CCNGG 3 cut(s) 567, 686, 1055
SduI GDGCHC 2 cut(s) 907, 1045
SfaNI GCATC 5 cut(s) 202, 270, 874, 1272, 1444
SgrAI CRCCGGYG 1 cut(s) 1424
SinI GGWCC 3 cut(s) 340, 869, 1300
SmiMI CAYNNNNRTG 3 cut(s) 59, 895, 1316
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SnaBI TACGTA 1 cut(s) 1120
SsiI CCGC 4 cut(s) 215, 477, 954, 1208
SspMI CTAG 1 cut(s) 749
StyD4I CCNGG 3 cut(s) 565, 684, 1053
StyI CCWWGG 2 cut(s) 248, 1146
TaaI ACNGT 5 cut(s) 40, 187, 390, 682, 724
TaiI ACGT 3 cut(s) 1122, 1341, 1389
TaqI TCGA 6 cut(s) 146, 165, 453, 536, 1347, 1361
TaqII GACCGA 1 cut(s) 227
TatI WGTACW 3 cut(s) 591, 1104, 1320
TauI GCSGC 1 cut(s) 1210
TfiI GAWTC 5 cut(s) 199, 367, 798, 1275, 1327
Tru1I TTAA 2 cut(s) 467, 1281
Tru9I TTAA 2 cut(s) 467, 1281
TscAI CASTG 4 cut(s) 237, 525, 608, 853
TseFI GTSAC 1 cut(s) 803
TseI GCWGC 3 cut(s) 258, 1142, 1253
Tsp45I GTSAC 1 cut(s) 803
TspDTI ATGAA 3 cut(s) 286, 473, 1241
TspGWI ACGGA 2 cut(s) 747, 897
TspRI CASTG 4 cut(s) 237, 525, 608, 853
Van91I CCANNNNNTGG 1 cut(s) 349
VpaK11BI GGWCC 3 cut(s) 340, 869, 1300
XapI RAATTY 1 cut(s) 853
XceI RCATGY 4 cut(s) 310, 428, 811, 983
XmiI GTMKAC 1 cut(s) 453
XspI CTAG 1 cut(s) 749
Zsp2I ATGCAT 1 cut(s) 1127
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.