Rw3G005960

Heparan-alpha-glucosaminide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003888
Physical Location & Seq
Forward (+)
5305779 .. 5309068
3290 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G005960.1

Sequence Viewer

Length: 1407 bp
ATGGCCGAAATCAAAGCCGAACCGGCTCTCCAGCACCATCTCATCGTATCGGAACCTGATATTTCCGACCAGAAGCCTTTAAAATCGAAACGTGTAGCTTCCCTCGACATCTTCCGAGGCCTCACTGTCTCTTTGATGATTTTGGTTGATGATGCCGGTGGAGAGTGGCCTATGATTGGGCATGCACCCTGGAATGGTTGCAATCTTGCTGACTTTGTGATGCCATTTTTCCTCTTCATTGTCGGAATGTCTATTGCACTTTCTCTTAAGAGAATACCAGACCAACTTTTGGCTGTCAAGAAGGTGATTCTTAGAACTCTCAAGCTCCTTTTCTGGGGTCTCCTATTACAAGGAGGTTTCTCACATGCTCCTGACAAACTAACATATGGTGTTGACATGAAAGAAATAAGATGGTGCGGGATTCTCCAGAGAATTGCTCTTGCATATCTGGTAGTGGCACTGATAGAAATTTTCTTGAGAAGTGCACAAACCAAAGATGTGACACCTGGCAGGCTCTCCATTTTCAAGTTATACTATTGGCAATGGCTGGTTGCAGCATGTGTTCTCACAACTTACTTTGCTGTTATTTATGGGACATATGTTCCTGATTGGCAATTTACCGTCCATGACGAAGGCAGTAGTGATTATGGGAAGAGTTTCACGGTATCTTGTGGCGTTAGAGGAGAACTGGATCCTCCTTGTAATGCAGTGGGATACATTGATAGACAAGTGTTGGGAATCAATCATATGTATCAGCATCCAGCTTGGAAAAGATCAAAAGCTTGCACTGAGAATTCTCCATATGGAGGACCTTTCCGAAATGGTGCTCCATCATGGTGCCATGCTCCTTTTGAACCTGAAGGAATTCTAAGCTCAATATCTGCAATTCTCTCCACAATCGTCGGAGTACATTTTGGACATGTGCTTATACATATGAAGGATCATCAAACTAGATTGAAGCATTGGATCCTAATGGGCTTTGCTCTCCTCATCCTAGGAATTGTTCTTCATTTCACTCATGCAATTCCTTCAAATAAACAGCTATATACGTTTAGTTACGTATGCATAACATCTGGAGCAGCAGCTTTGGTGTTTTCAGCCTTCTACATCATGGTTGATATCTGGGATATAAAGTACCTGTTTCTTCTTCCCCTGCAATGGATCGGCATGAATGCTATGCTTGTCTACGTTATGGCAGCTGAAGGCATCTTTGCAGGGTTCTTCAACGGGTGGTATTACAAGGACCCCCATAATACGCTAATATATTGGATCCAGAAGCACATTTTTGTTGGGGTTTGGCATTCAAGGAGAGTAGGCATTCTACTCTATGTAATCTTCGCAGAGATCTTGTTTTGGGGCATTGTTGCTGGCATTTTGCATTGGTTGGGCATATATTGGAAGCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624 DUF5009

Protein Analysis

468

Amino Acids

53.05

Weight (kDa)

8.62

Isoelectric Point (pI)

35.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 837
AccB7I CCANNNNNTGG 1 cut(s) 289
AccI GTMKAC 1 cut(s) 1185
AciI CCGC 1 cut(s) 417
AclWI GGATC 8 cut(s) 686, 699, 948, 961, 974, 1169, 1264, 1277
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 468, 793, 864
AcuI CTGAAG 2 cut(s) 879, 1221
AfaI GTAC 2 cut(s) 909, 1136
AfiI CCNNNNNNNGG 6 cut(s) 176, 194, 289, 334, 806, 1158
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 2 cut(s) 91, 919
AgsI TTSAA 6 cut(s) 526, 854, 958, 1032, 1225, 1305
AjnI CCWGG 2 cut(s) 188, 505
AloI GAACNNNNNNTCC 2 cut(s) 12, 44
AluBI AGCT 9 cut(s) 98, 325, 764, 782, 873, 1042, 1085, 1199, 1402
AluI AGCT 9 cut(s) 98, 325, 764, 782, 873, 1042, 1085, 1199, 1402
Alw21I GWGCWC 2 cut(s) 487, 829
Alw26I GTCTC 2 cut(s) 133, 344
Alw44I GTGCAC 1 cut(s) 483
AlwI GGATC 8 cut(s) 686, 699, 948, 961, 974, 1169, 1264, 1277
AoxI GGCC 3 cut(s) 3, 118, 167
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 4 cut(s) 554, 1079, 1082, 1196
ApoI RAATTY 3 cut(s) 468, 793, 864
Asp700I GAANNNNTTC 2 cut(s) 656, 864
AspA2I CCTAGG 1 cut(s) 994
AspS9I GGNCC 2 cut(s) 809, 1243
AsuHPI GGTGA 1 cut(s) 316
AvaII GGWCC 2 cut(s) 809, 1243
AvrII CCTAGG 1 cut(s) 994
BaeGI GKGCMC 1 cut(s) 487
BamHI GGATCC 3 cut(s) 691, 966, 1269
BanI GGYRCC 1 cut(s) 837
Bbv12I GWGCWC 2 cut(s) 487, 829
BbvI GCAGC 4 cut(s) 566, 1091, 1094, 1208
BccI CCATC 3 cut(s) 45, 405, 838
BciT130I CCWGG 2 cut(s) 190, 507
BciVI GTATCC 1 cut(s) 707
BcoDI GTCTC 2 cut(s) 133, 344
BfaI CTAG 2 cut(s) 951, 995
BfrI CTTAAG 1 cut(s) 266
BfuI GTATCC 1 cut(s) 707
BglI GCCNNNNNGGC 1 cut(s) 23
BglII AGATCT 1 cut(s) 1344
BisI GCNGC 4 cut(s) 555, 1080, 1083, 1197
BlnI CCTAGG 1 cut(s) 994
BlsI GCNGC 4 cut(s) 556, 1081, 1084, 1198
Bme1390I CCNGG 2 cut(s) 190, 507
Bme18I GGWCC 2 cut(s) 809, 1243
BmgT120I GGNCC 2 cut(s) 809, 1243
BmiI GGNNCC 6 cut(s) 54, 693, 839, 968, 1245, 1271
BmrFI CCNGG 2 cut(s) 190, 507
BmsI GCATC 4 cut(s) 142, 210, 766, 1215
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 3 cut(s) 14, 410, 1095
BpuEI CTTGAG 2 cut(s) 305, 496
BsaAI YACGTR 1 cut(s) 1060
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 115, 188, 994
BsaXI ACNNNNNCTCC 2 cut(s) 12, 42
Bsc4I CCNNNNNNNGG 6 cut(s) 176, 194, 289, 334, 806, 1158
Bse118I RCCGGY 2 cut(s) 22, 155
Bse1I ACTGG 1 cut(s) 693
Bse3DI GCAATG 2 cut(s) 548, 1163
BseBI CCWGG 2 cut(s) 190, 507
BseDI CCNNGG 3 cut(s) 115, 188, 994
BseGI GGATG 2 cut(s) 757, 990
BseLI CCNNNNNNNGG 6 cut(s) 176, 194, 289, 334, 806, 1158
BseMI GCAATG 2 cut(s) 548, 1163
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 1 cut(s) 693
BseRI GAGGAG 2 cut(s) 696, 977
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 4 cut(s) 566, 1091, 1094, 1208
BshFI GGCC 3 cut(s) 5, 120, 169
BshNI GGYRCC 1 cut(s) 837
BsiHKAI GWGCWC 2 cut(s) 487, 829
BsiSI CCGG 2 cut(s) 23, 156
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 6 cut(s) 176, 194, 289, 334, 806, 1158
BsmAI GTCTC 2 cut(s) 133, 344
BsmFI GGGAC 1 cut(s) 607
BsmI GAATGC 3 cut(s) 1177, 1300, 1317
BsnI GGCC 3 cut(s) 5, 120, 169
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 487, 829
Bsp143I GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
BspACI CCGC 1 cut(s) 417
BspANI GGCC 3 cut(s) 5, 120, 169
BspCNI CTCAG 1 cut(s) 781
BspLI GGNNCC 6 cut(s) 54, 693, 839, 968, 1245, 1271
BspPI GGATC 8 cut(s) 686, 699, 948, 961, 974, 1169, 1264, 1277
BspT107I GGYRCC 1 cut(s) 837
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 2 cut(s) 548, 1163
BsrFI RCCGGY 2 cut(s) 22, 155
BsrI ACTGG 1 cut(s) 693
BssAI RCCGGY 2 cut(s) 22, 155
BssECI CCNNGG 3 cut(s) 115, 188, 994
BssMI GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
BssT1I CCWWGG 1 cut(s) 994
Bst2UI CCWGG 2 cut(s) 190, 507
Bst4CI ACNGT 3 cut(s) 127, 622, 664
Bst6I CTCTTC 2 cut(s) 239, 647
BstAFI CTTAAG 1 cut(s) 266
BstBAI YACGTR 1 cut(s) 1060
BstC8I GCNNGC 4 cut(s) 183, 512, 784, 1369
BstDEI CTNAG 3 cut(s) 311, 789, 869
BstF5I GGATG 2 cut(s) 757, 990
BstKTI GATC 7 cut(s) 694, 776, 943, 969, 1164, 1272, 1347
BstMAI GTCTC 2 cut(s) 133, 344
BstMBI GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 190, 507
BstNSI RCATGY 4 cut(s) 185, 368, 561, 923
BstSCI CCNGG 2 cut(s) 188, 505
BstSLI GKGCMC 1 cut(s) 487
BstSNI TACGTA 1 cut(s) 1060
BstV1I GCAGC 4 cut(s) 566, 1091, 1094, 1208
BstX2I RGATCY 4 cut(s) 691, 966, 1269, 1344
BstYI RGATCY 4 cut(s) 691, 966, 1269, 1344
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 3 cut(s) 5, 120, 169
BtsCI GGATG 2 cut(s) 757, 990
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 4 cut(s) 123, 458, 714, 786
Cac8I GCNNGC 4 cut(s) 183, 512, 784, 1369
Cfr10I RCCGGY 2 cut(s) 22, 155
Cfr13I GGNCC 2 cut(s) 809, 1243
Csp6I GTAC 2 cut(s) 908, 1135
CviQI GTAC 2 cut(s) 908, 1135
DdeI CTNAG 3 cut(s) 311, 789, 869
DpnI GATC 7 cut(s) 693, 775, 942, 968, 1163, 1271, 1346
DpnII GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
DraI TTTAAA 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 239, 647
EarI CTCTTC 2 cut(s) 239, 647
Eco105I TACGTA 1 cut(s) 1060
Eco130I CCWWGG 1 cut(s) 994
Eco147I AGGCCT 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 344
Eco32I GATATC 1 cut(s) 1120
Eco47I GGWCC 2 cut(s) 809, 1243
Eco57I CTGAAG 2 cut(s) 879, 1221
EcoO109I RGGNCCY 2 cut(s) 809, 1243
EcoRI GAATTC 2 cut(s) 793, 864
EcoRII CCWGG 2 cut(s) 188, 505
EcoRV GATATC 1 cut(s) 1120
EcoT14I CCWWGG 1 cut(s) 994
EcoT22I ATGCAT 1 cut(s) 1067
ErhI CCWWGG 1 cut(s) 994
FaqI GGGAC 1 cut(s) 607
FauI CCCGC 1 cut(s) 410
FauNDI CATATG 5 cut(s) 385, 598, 747, 802, 933
FblI GTMKAC 1 cut(s) 1185
Fnu4HI GCNGC 4 cut(s) 555, 1080, 1083, 1197
FokI GGATG 2 cut(s) 744, 977
Fsp4HI GCNGC 4 cut(s) 555, 1080, 1083, 1197
FspBI CTAG 2 cut(s) 951, 995
GluI GCNGC 4 cut(s) 555, 1080, 1083, 1197
GsuI CTGGAG 3 cut(s) 14, 410, 1095
HaeIII GGCC 3 cut(s) 5, 120, 169
HapII CCGG 2 cut(s) 23, 156
HincII GTYRAC 1 cut(s) 394
HindII GTYRAC 1 cut(s) 394
HindIII AAGCTT 2 cut(s) 780, 1400
HinfI GANTC 3 cut(s) 307, 421, 738
HpaII CCGG 2 cut(s) 23, 156
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 3 cut(s) 394, 485, 1186
Hpy188I TCNGA 6 cut(s) 52, 67, 116, 245, 818, 905
Hpy188III TCNNGA 7 cut(s) 298, 371, 427, 475, 605, 1074, 1273
Hpy8I GTNNAC 3 cut(s) 394, 485, 1186
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 7 cut(s) 295, 626, 854, 931, 1038, 1111, 1196
HpyCH4III ACNGT 3 cut(s) 127, 622, 664
HpyCH4IV ACGT 4 cut(s) 91, 1049, 1059, 1188
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 3 cut(s) 311, 789, 869
HpySE526I ACGT 4 cut(s) 91, 1049, 1059, 1188
Kzo9I GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
LmnI GCTCC 5 cut(s) 330, 373, 832, 850, 1076
Lsp1109I GCAGC 4 cut(s) 566, 1091, 1094, 1208
LweI GCATC 4 cut(s) 142, 210, 766, 1215
MaeI CTAG 2 cut(s) 951, 995
MaeII ACGT 4 cut(s) 91, 1049, 1059, 1188
MaeIII GTNAC 2 cut(s) 499, 1055
MalI GATC 7 cut(s) 693, 775, 942, 968, 1163, 1271, 1346
MboI GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
MboII GAAGA 8 cut(s) 103, 226, 664, 998, 1136, 1139, 1213, 1327
MflI RGATCY 4 cut(s) 691, 966, 1269, 1344
MhlI GDGCHC 2 cut(s) 487, 829
MluCI AATT 8 cut(s) 432, 468, 614, 793, 864, 885, 999, 1023
MmeI TCCRAC 3 cut(s) 90, 223, 883
MnlI CCTC 9 cut(s) 110, 113, 131, 242, 347, 674, 705, 800, 998
Mph1103I ATGCAT 1 cut(s) 1067
MroXI GAANNNNTTC 2 cut(s) 656, 864
MseI TTAA 3 cut(s) 80, 267, 1405
MslI CAYNNNNRTG 1 cut(s) 835
MspA1I CMGCKG 1 cut(s) 1199
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 2 cut(s) 23, 156
MspR9I CCNGG 2 cut(s) 190, 507
Mva1269I GAATGC 3 cut(s) 1177, 1300, 1317
MvaI CCWGG 2 cut(s) 190, 507
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeI CATATG 5 cut(s) 385, 598, 747, 802, 933
NdeII GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
NlaIV GGNNCC 6 cut(s) 54, 693, 839, 968, 1245, 1271
NmuCI GTSAC 1 cut(s) 499
NsiI ATGCAT 1 cut(s) 1067
NspI RCATGY 4 cut(s) 185, 368, 561, 923
PaeI GCATGC 1 cut(s) 185
PceI AGGCCT 1 cut(s) 120
PciI ACATGT 1 cut(s) 919
PctI GAATGC 3 cut(s) 1177, 1300, 1317
PdmI GAANNNNTTC 2 cut(s) 656, 864
PfeI GAWTC 3 cut(s) 307, 421, 738
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 4 cut(s) 556, 1081, 1084, 1198
Ppu21I YACGTR 1 cut(s) 1060
PpuMI RGGWCCY 2 cut(s) 809, 1243
PscI ACATGT 1 cut(s) 919
Psp5II RGGWCCY 2 cut(s) 809, 1243
Psp6I CCWGG 2 cut(s) 188, 505
PspGI CCWGG 2 cut(s) 188, 505
PspN4I GGNNCC 6 cut(s) 54, 693, 839, 968, 1245, 1271
PspPI GGNCC 2 cut(s) 809, 1243
PspPPI RGGWCCY 2 cut(s) 809, 1243
PsuI RGATCY 4 cut(s) 691, 966, 1269, 1344
PvuII CAGCTG 1 cut(s) 1199
RsaI GTAC 2 cut(s) 909, 1136
RsaNI GTAC 2 cut(s) 908, 1135
RseI CAYNNNNRTG 1 cut(s) 835
SaqAI TTAA 3 cut(s) 80, 267, 1405
SatI GCNGC 4 cut(s) 555, 1080, 1083, 1197
Sau3AI GATC 7 cut(s) 691, 773, 940, 966, 1161, 1269, 1344
Sau96I GGNCC 2 cut(s) 809, 1243
ScrFI CCNGG 2 cut(s) 190, 507
SduI GDGCHC 2 cut(s) 487, 829
SfaNI GCATC 4 cut(s) 142, 210, 766, 1215
SinI GGWCC 2 cut(s) 809, 1243
SmiMI CAYNNNNRTG 1 cut(s) 835
SmlI CTYRAG 3 cut(s) 266, 320, 475
SmoI CTYRAG 3 cut(s) 266, 320, 475
SnaBI TACGTA 1 cut(s) 1060
SphI GCATGC 1 cut(s) 185
Sse9I AATT 8 cut(s) 432, 468, 614, 793, 864, 885, 999, 1023
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 1 cut(s) 417
SspMI CTAG 2 cut(s) 951, 995
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 188, 505
StyI CCWWGG 1 cut(s) 994
TaaI ACNGT 3 cut(s) 127, 622, 664
TaiI ACGT 4 cut(s) 94, 1052, 1062, 1191
TaqI TCGA 2 cut(s) 86, 105
TasI AATT 8 cut(s) 432, 468, 614, 793, 864, 885, 999, 1023
TatI WGTACW 1 cut(s) 907
TfiI GAWTC 3 cut(s) 307, 421, 738
Tru1I TTAA 3 cut(s) 80, 267, 1405
Tru9I TTAA 3 cut(s) 80, 267, 1405
TscAI CASTG 4 cut(s) 130, 465, 714, 793
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 4 cut(s) 554, 1079, 1082, 1196
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 5 cut(s) 226, 413, 950, 998, 1184
TspRI CASTG 4 cut(s) 130, 465, 714, 793
Van91I CCANNNNNTGG 1 cut(s) 289
Vha464I CTTAAG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 2 cut(s) 809, 1243
XapI RAATTY 3 cut(s) 468, 793, 864
XceI RCATGY 4 cut(s) 185, 368, 561, 923
XmaJI CCTAGG 1 cut(s) 994
XmiI GTMKAC 1 cut(s) 1185
XmnI GAANNNNTTC 2 cut(s) 656, 864
XspI CTAG 2 cut(s) 951, 995
Zsp2I ATGCAT 1 cut(s) 1067
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.