Rh3BG071900

Heparan-alpha-glucosaminide

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0003888
Physical Location & Seq
Forward (+)
4826314 .. 4828781
2468 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG071900.1

Sequence Viewer

Length: 948 bp
ATGGCCGAAATCAAAGCCGAACCGGCTCACCAGCACCATCTCATCGTATCGGAACCTGATATTTCCGACCAGAAGCCTTTAAAACCGAAACGTGTAGCTTCCCTCGACATCTTCCGAGGCCTCACTGTCTCTTTGATGATTTTGGTTGATGATGCCGGTGGAGAGTGGCCTATGATTGGGCATGCACCCTGGAATGGTTGCAATCTTGCTGACTTTGTGATGCCATTTTTCCTCTTGATTGTGGGAATGTCTATTGCACTTTCTCTTAAGAGAATACCAGACCAACTTTTGGCTGTCAAGAAGGTGATTCTTAGAACTCTCAAGCTCCTTTTCTGGGGTCTCCTATTACAAGGAGGTTTCTCACATGCTCCTGACAAACTAACATATGGTGTTGACATGAAAGAAATAAGATGGTGCGGGATTCTCCAGAGAATTGCTCTTGCATATCTGGTAGTGGCACTGATAGAAATTTTCTTGAGAAGTGCACAAACCAAAGATGTGACACCTGGCAGGCTCTCCATTTTCAAGTTATACTATTGGCAATGGCTGGTTGCAGCATGTGTTCTCACAACTTACTTTGCTGTTATTTATGGGACATATGTTCCTGATTGGCAATTTACCGTCCATGACGAAGGCAGTAGTGATTATGGGAAGAGTTTCACGGTATCTTGTGGCGTTAGAGGAGAACTGGATCCTCCTTGTAATGCAGTGGGATACATTGATAGACAAGTGTTGGGAATCAATCATATGTATCAGCATCCAGCTTGGAAAAGATCAAAAGCTTGCACTGAGAATTCTCCATATGGAGGACCTTTCCGAAGTGGTGCTCCATCATGGTGCCATGCTCCTTTTGAACCTGAAGGAATTCTAAGCTCAATATCTGCAATTCTCTCCACAATCGTCGGAGTACATTTTGGACATGTGCTTATACATATGAAGGTTAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00531 ko01100 ko04142 map00531 map01100 map04142
Pfam Domains
Protein Families
DUF1624 DUF5009

Protein Analysis

315

Amino Acids

35.11

Weight (kDa)

8.12

Isoelectric Point (pI)

39.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HGSNAT_cat PF07786 31 - 155 2.3e-06 Heparan-alpha-glucosaminide N-acetyltransferase, catalytic
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 837
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 1 cut(s) 417
AclWI GGATC 2 cut(s) 686, 699
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 3 cut(s) 468, 793, 864
AcuI CTGAAG 1 cut(s) 879
AfaI GTAC 1 cut(s) 909
AfiI CCNNNNNNNGG 5 cut(s) 176, 194, 289, 334, 806
AflII CTTAAG 1 cut(s) 266
AflIII ACRYGT 2 cut(s) 91, 919
AgsI TTSAA 2 cut(s) 526, 854
AjnI CCWGG 2 cut(s) 188, 505
AluBI AGCT 5 cut(s) 98, 325, 764, 782, 873
AluI AGCT 5 cut(s) 98, 325, 764, 782, 873
Alw21I GWGCWC 2 cut(s) 487, 829
Alw26I GTCTC 2 cut(s) 133, 344
Alw44I GTGCAC 1 cut(s) 483
AlwI GGATC 2 cut(s) 686, 699
AoxI GGCC 3 cut(s) 3, 118, 167
ApaLI GTGCAC 1 cut(s) 483
ApeKI GCWGC 1 cut(s) 554
ApoI RAATTY 3 cut(s) 468, 793, 864
Asp700I GAANNNNTTC 2 cut(s) 656, 864
AspS9I GGNCC 1 cut(s) 809
AsuHPI GGTGA 2 cut(s) 20, 316
AvaII GGWCC 1 cut(s) 809
BaeGI GKGCMC 1 cut(s) 487
BamHI GGATCC 1 cut(s) 691
BanI GGYRCC 1 cut(s) 837
Bbv12I GWGCWC 2 cut(s) 487, 829
BbvI GCAGC 1 cut(s) 566
BccI CCATC 3 cut(s) 45, 405, 838
BciT130I CCWGG 2 cut(s) 190, 507
BciVI GTATCC 1 cut(s) 707
BcoDI GTCTC 2 cut(s) 133, 344
BfrI CTTAAG 1 cut(s) 266
BfuI GTATCC 1 cut(s) 707
BglI GCCNNNNNGGC 1 cut(s) 23
BisI GCNGC 1 cut(s) 555
BlsI GCNGC 1 cut(s) 556
Bme1390I CCNGG 2 cut(s) 190, 507
Bme18I GGWCC 1 cut(s) 809
BmgT120I GGNCC 1 cut(s) 809
BmiI GGNNCC 3 cut(s) 54, 693, 839
BmrFI CCNGG 2 cut(s) 190, 507
BmsI GCATC 3 cut(s) 142, 210, 766
BplI GAGNNNNNCTC 2 cut(s) 421, 453
BpmI CTGGAG 1 cut(s) 410
BpuEI CTTGAG 2 cut(s) 305, 496
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 2 cut(s) 115, 188
Bsc4I CCNNNNNNNGG 5 cut(s) 176, 194, 289, 334, 806
Bse118I RCCGGY 2 cut(s) 22, 155
Bse1I ACTGG 1 cut(s) 693
Bse3DI GCAATG 1 cut(s) 548
BseBI CCWGG 2 cut(s) 190, 507
BseDI CCNNGG 2 cut(s) 115, 188
BseGI GGATG 1 cut(s) 757
BseLI CCNNNNNNNGG 5 cut(s) 176, 194, 289, 334, 806
BseMI GCAATG 1 cut(s) 548
BseMII CTCAG 1 cut(s) 780
BseNI ACTGG 1 cut(s) 693
BseRI GAGGAG 1 cut(s) 696
BseSI GKGCMC 1 cut(s) 487
BseXI GCAGC 1 cut(s) 566
BshFI GGCC 3 cut(s) 5, 120, 169
BshNI GGYRCC 1 cut(s) 837
BsiHKAI GWGCWC 2 cut(s) 487, 829
BsiSI CCGG 2 cut(s) 23, 156
BslFI GGGAC 1 cut(s) 607
BslI CCNNNNNNNGG 5 cut(s) 176, 194, 289, 334, 806
BsmAI GTCTC 2 cut(s) 133, 344
BsmFI GGGAC 1 cut(s) 607
BsnI GGCC 3 cut(s) 5, 120, 169
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 2 cut(s) 487, 829
Bsp143I GATC 2 cut(s) 691, 773
BspACI CCGC 1 cut(s) 417
BspANI GGCC 3 cut(s) 5, 120, 169
BspCNI CTCAG 1 cut(s) 781
BspLI GGNNCC 3 cut(s) 54, 693, 839
BspPI GGATC 2 cut(s) 686, 699
BspT107I GGYRCC 1 cut(s) 837
BspTI CTTAAG 1 cut(s) 266
BspTNI GGTCTC 1 cut(s) 344
BsrDI GCAATG 1 cut(s) 548
BsrFI RCCGGY 2 cut(s) 22, 155
BsrI ACTGG 1 cut(s) 693
BssAI RCCGGY 2 cut(s) 22, 155
BssECI CCNNGG 2 cut(s) 115, 188
BssMI GATC 2 cut(s) 691, 773
Bst2UI CCWGG 2 cut(s) 190, 507
Bst4CI ACNGT 3 cut(s) 127, 622, 664
Bst6I CTCTTC 1 cut(s) 647
BstAFI CTTAAG 1 cut(s) 266
BstC8I GCNNGC 3 cut(s) 183, 512, 784
BstDEI CTNAG 3 cut(s) 311, 789, 869
BstF5I GGATG 1 cut(s) 757
BstKTI GATC 2 cut(s) 694, 776
BstMAI GTCTC 2 cut(s) 133, 344
BstMBI GATC 2 cut(s) 691, 773
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 190, 507
BstNSI RCATGY 4 cut(s) 185, 368, 561, 923
BstSCI CCNGG 2 cut(s) 188, 505
BstSLI GKGCMC 1 cut(s) 487
BstV1I GCAGC 1 cut(s) 566
BstX2I RGATCY 1 cut(s) 691
BstYI RGATCY 1 cut(s) 691
BsuI GTATCC 1 cut(s) 707
BsuRI GGCC 3 cut(s) 5, 120, 169
BtsCI GGATG 1 cut(s) 757
BtsI GCAGTG 1 cut(s) 714
BtsIMutI CAGTG 4 cut(s) 123, 458, 714, 786
Cac8I GCNNGC 3 cut(s) 183, 512, 784
Cfr10I RCCGGY 2 cut(s) 22, 155
Cfr13I GGNCC 1 cut(s) 809
Csp6I GTAC 1 cut(s) 908
CviAII CATG 8 cut(s) 182, 365, 397, 558, 626, 834, 842, 920
CviQI GTAC 1 cut(s) 908
DdeI CTNAG 3 cut(s) 311, 789, 869
DpnI GATC 2 cut(s) 693, 775
DpnII GATC 2 cut(s) 691, 773
DraI TTTAAA 1 cut(s) 81
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 647
EarI CTCTTC 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 120
Eco31I GGTCTC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 809
Eco57I CTGAAG 1 cut(s) 879
EcoO109I RGGNCCY 1 cut(s) 809
EcoRI GAATTC 2 cut(s) 793, 864
EcoRII CCWGG 2 cut(s) 188, 505
FaeI CATG 8 cut(s) 185, 368, 400, 561, 629, 837, 845, 923
FaqI GGGAC 1 cut(s) 607
FatI CATG 8 cut(s) 181, 364, 396, 557, 625, 833, 841, 919
FauI CCCGC 1 cut(s) 410
FauNDI CATATG 5 cut(s) 385, 598, 747, 802, 933
Fnu4HI GCNGC 1 cut(s) 555
FokI GGATG 1 cut(s) 744
Fsp4HI GCNGC 1 cut(s) 555
GluI GCNGC 1 cut(s) 555
GsuI CTGGAG 1 cut(s) 410
HaeIII GGCC 3 cut(s) 5, 120, 169
HapII CCGG 2 cut(s) 23, 156
Hin1II CATG 8 cut(s) 185, 368, 400, 561, 629, 837, 845, 923
HincII GTYRAC 2 cut(s) 394, 943
HindII GTYRAC 2 cut(s) 394, 943
HindIII AAGCTT 1 cut(s) 780
HinfI GANTC 3 cut(s) 307, 421, 738
HpaI GTTAAC 1 cut(s) 943
HpaII CCGG 2 cut(s) 23, 156
HphI GGTGA 2 cut(s) 20, 316
Hpy166II GTNNAC 3 cut(s) 394, 485, 943
Hpy188I TCNGA 5 cut(s) 52, 67, 116, 818, 905
Hpy188III TCNNGA 6 cut(s) 235, 298, 371, 427, 475, 605
Hpy8I GTNNAC 3 cut(s) 394, 485, 943
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 4 cut(s) 295, 626, 854, 931
HpyCH4III ACNGT 3 cut(s) 127, 622, 664
HpyCH4IV ACGT 1 cut(s) 91
HpyCH4V TGCA 9 cut(s) 185, 201, 257, 443, 485, 554, 707, 786, 884
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 3 cut(s) 311, 789, 869
HpySE526I ACGT 1 cut(s) 91
Hsp92II CATG 8 cut(s) 185, 368, 400, 561, 629, 837, 845, 923
KspAI GTTAAC 1 cut(s) 943
Kzo9I GATC 2 cut(s) 691, 773
LmnI GCTCC 4 cut(s) 330, 373, 832, 850
Lsp1109I GCAGC 1 cut(s) 566
LweI GCATC 3 cut(s) 142, 210, 766
MaeII ACGT 1 cut(s) 91
MaeIII GTNAC 1 cut(s) 499
MalI GATC 2 cut(s) 693, 775
MboI GATC 2 cut(s) 691, 773
MboII GAAGA 2 cut(s) 103, 664
MflI RGATCY 1 cut(s) 691
MhlI GDGCHC 2 cut(s) 487, 829
MluCI AATT 6 cut(s) 432, 468, 614, 793, 864, 885
MmeI TCCRAC 2 cut(s) 90, 883
MnlI CCTC 8 cut(s) 110, 113, 131, 242, 347, 674, 705, 800
MroXI GAANNNNTTC 2 cut(s) 656, 864
MseI TTAA 3 cut(s) 80, 267, 942
MslI CAYNNNNRTG 1 cut(s) 835
MspCI CTTAAG 1 cut(s) 266
MspI CCGG 2 cut(s) 23, 156
MspR9I CCNGG 2 cut(s) 190, 507
MvaI CCWGG 2 cut(s) 190, 507
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeI CATATG 5 cut(s) 385, 598, 747, 802, 933
NdeII GATC 2 cut(s) 691, 773
NlaIII CATG 8 cut(s) 185, 368, 400, 561, 629, 837, 845, 923
NlaIV GGNNCC 3 cut(s) 54, 693, 839
NmuCI GTSAC 1 cut(s) 499
NspI RCATGY 4 cut(s) 185, 368, 561, 923
PaeI GCATGC 1 cut(s) 185
PceI AGGCCT 1 cut(s) 120
PciI ACATGT 1 cut(s) 919
PdmI GAANNNNTTC 2 cut(s) 656, 864
PfeI GAWTC 3 cut(s) 307, 421, 738
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 1 cut(s) 556
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 919
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 188, 505
PspGI CCWGG 2 cut(s) 188, 505
PspN4I GGNNCC 3 cut(s) 54, 693, 839
PspPI GGNCC 1 cut(s) 809
PspPPI RGGWCCY 1 cut(s) 809
PsuI RGATCY 1 cut(s) 691
RsaI GTAC 1 cut(s) 909
RsaNI GTAC 1 cut(s) 908
RseI CAYNNNNRTG 1 cut(s) 835
SaqAI TTAA 3 cut(s) 80, 267, 942
SatI GCNGC 1 cut(s) 555
Sau3AI GATC 2 cut(s) 691, 773
Sau96I GGNCC 1 cut(s) 809
ScrFI CCNGG 2 cut(s) 190, 507
SduI GDGCHC 2 cut(s) 487, 829
SfaNI GCATC 3 cut(s) 142, 210, 766
SinI GGWCC 1 cut(s) 809
SmiMI CAYNNNNRTG 1 cut(s) 835
SmlI CTYRAG 3 cut(s) 266, 320, 475
SmoI CTYRAG 3 cut(s) 266, 320, 475
SphI GCATGC 1 cut(s) 185
Sse9I AATT 6 cut(s) 432, 468, 614, 793, 864, 885
SseBI AGGCCT 1 cut(s) 120
SsiI CCGC 1 cut(s) 417
StuI AGGCCT 1 cut(s) 120
StyD4I CCNGG 2 cut(s) 188, 505
TaaI ACNGT 3 cut(s) 127, 622, 664
TaiI ACGT 1 cut(s) 94
TaqI TCGA 1 cut(s) 105
TasI AATT 6 cut(s) 432, 468, 614, 793, 864, 885
TatI WGTACW 1 cut(s) 907
TfiI GAWTC 3 cut(s) 307, 421, 738
Tru1I TTAA 3 cut(s) 80, 267, 942
Tru9I TTAA 3 cut(s) 80, 267, 942
TscAI CASTG 4 cut(s) 130, 465, 714, 793
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 1 cut(s) 554
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 1 cut(s) 413
TspRI CASTG 4 cut(s) 130, 465, 714, 793
Van91I CCANNNNNTGG 1 cut(s) 289
Vha464I CTTAAG 1 cut(s) 266
VneI GTGCAC 1 cut(s) 483
VpaK11BI GGWCC 1 cut(s) 809
XapI RAATTY 3 cut(s) 468, 793, 864
XceI RCATGY 4 cut(s) 185, 368, 561, 923
XmnI GAANNNNTTC 2 cut(s) 656, 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.