MD04G1204800.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013410
Physical Location & Seq
Reverse (-)
29104941 .. 29106157
1217 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1204800.v1.1.491

Sequence Viewer

Length: 804 bp
ATGGGTTGCTGCGGAGACGAAGAAAACGACGACGACATCCACAATCCTCTCAACGCCGGCGCCGACACCACCTCCACCCAGACCCTCGGCGAATACTCCGACTCTACCACCACCATATCCCCGATGAACTCTCACTTCTCGGCCTTGACTTGCCGCGACACCCTCCGCCTCATCTTCGAGAAGCTCCCGATTCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGGGTCTGGAATTCCGTGGCCTCCGATCAGGAGATCGTCGATAAAGCCTTCAAGTCCCCTTGGAATCTGAAGGAGGTGATTGGAAAGCCCTCGTCTTTAAGCTTCTGGAGGGACAATTCGATCGGAAAATTCGCCATCTCGCACCGGATTGTCCGCGGCGACAGCGTCGCCAGCCTCGCCGTCAAGTATTCCGTTCAGGTGATGGACATAAAACGATTGAACAACATGATGAGCGAGCACGGAATATACTCTCGGGAGAGATTGCTGATTCCGATAAGCAACGCGGAGATGCTTTCGGATGCAACCTGCTATGTAGAAGTGGATAGTTATGCAAAGAGAGAAGTGGCTGTGTTGTATTTGGAAGGCGGCCCCGACCCGAAAACCATCGGCGGCAGCAGCTCTTCATGCTCTGTCTTGCAGACCAAGAAAAGAGTGGTTGAATCGCTGAGGAGAAGTCTGCAAGTAGATGATGCAACTGTGAACTACTACTTGTCCATTGCCGGCGGCGATCCAAGAGCTGCTATCTCTCAGTTCTCCCAGGACCTTAGGTGGGAGACCCACACTCACCTCGGCTTTGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

268

Amino Acids

29.52

Weight (kDa)

5.55

Isoelectric Point (pI)

40.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 52 - 86 1e-06 F-box domain
F-box-like PF12937 55 - 84 9.1e-07 F-box-like
LysM PF01476 122 - 165 2.1e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 536
AccB1I GGYRCC 1 cut(s) 59
AccII CGCG 3 cut(s) 156, 378, 506
AciI CCGC 9 cut(s) 12, 154, 166, 376, 378, 506, 588, 612, 726
AclWI GGATC 1 cut(s) 725
AcsI RAATTY 2 cut(s) 232, 350
AcuI CTGAAG 1 cut(s) 311
AcyI GRCGYC 1 cut(s) 60
AfiI CCNNNNNNNGG 1 cut(s) 772
AgsI TTSAA 3 cut(s) 274, 442, 662
AjnI CCWGG 1 cut(s) 759
AluBI AGCT 5 cut(s) 184, 213, 324, 621, 740
AluI AGCT 5 cut(s) 184, 213, 324, 621, 740
Alw21I GWGCWC 1 cut(s) 462
Alw26I GTCTC 2 cut(s) 9, 770
AlwI GGATC 1 cut(s) 725
Ama87I CYCGRG 1 cut(s) 474
AoxI GGCC 3 cut(s) 141, 240, 589
ApeKI GCWGC 5 cut(s) 9, 213, 615, 618, 740
ApoI RAATTY 2 cut(s) 232, 350
AspLEI GCGC 1 cut(s) 62
AspS9I GGNCC 2 cut(s) 590, 763
AsuC2I CCSGG 1 cut(s) 224
AsuHPI GGTGA 3 cut(s) 310, 433, 779
AvaI CYCGRG 1 cut(s) 474
AvaII GGWCC 1 cut(s) 763
AxyI CCTNAGG 1 cut(s) 767
BanI GGYRCC 1 cut(s) 59
Bbv12I GWGCWC 1 cut(s) 462
BbvCI CCTCAGC 1 cut(s) 668
BbvI GCAGC 4 cut(s) 200, 627, 630, 727
BccI CCATC 3 cut(s) 365, 418, 614
BceAI ACGGC 1 cut(s) 386
BciT130I CCWGG 1 cut(s) 761
BcnI CCSGG 1 cut(s) 224
BcoDI GTCTC 2 cut(s) 9, 770
BfoI RGCGCY 1 cut(s) 63
BfuAI ACCTGC 1 cut(s) 536
Bme1390I CCNGG 2 cut(s) 224, 761
Bme18I GGWCC 1 cut(s) 763
BmeT110I CYCGRG 1 cut(s) 474
BmgT120I GGNCC 2 cut(s) 590, 763
BmiI GGNNCC 2 cut(s) 61, 592
BmrFI CCNGG 2 cut(s) 224, 761
BmsI GCATC 3 cut(s) 501, 511, 682
BpmI CTGGAG 2 cut(s) 193, 349
Bpu10I CCTNAGC 1 cut(s) 668
BpuMI CCSGG 1 cut(s) 224
BsaHI GRCGYC 1 cut(s) 60
BsaI GGTCTC 1 cut(s) 770
BsaJI CCNNGG 6 cut(s) 85, 237, 281, 376, 759, 790
BsaWI WCCGGW 1 cut(s) 366
BsaXI ACNNNNNCTCC 2 cut(s) 56, 86
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse118I RCCGGY 2 cut(s) 56, 722
Bse21I CCTNAGG 1 cut(s) 767
Bse3DI GCAATG 1 cut(s) 717
BseBI CCWGG 1 cut(s) 761
BseDI CCNNGG 6 cut(s) 85, 237, 281, 376, 759, 790
BseGI GGATG 2 cut(s) 36, 526
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMI GCAATG 1 cut(s) 717
BseMII CTCAG 2 cut(s) 659, 764
BseRI GAGGAG 1 cut(s) 685
BseXI GCAGC 4 cut(s) 200, 627, 630, 727
Bsh1236I CGCG 3 cut(s) 156, 378, 506
Bsh1285I CGRYCG 1 cut(s) 345
BshFI GGCC 3 cut(s) 143, 242, 591
BshNI GGYRCC 1 cut(s) 59
BsiEI CGRYCG 1 cut(s) 345
BsiHKAI GWGCWC 1 cut(s) 462
BsiHKCI CYCGRG 1 cut(s) 474
BsiSI CCGG 4 cut(s) 57, 223, 367, 723
BslFI GGGAC 2 cut(s) 262, 347
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 2 cut(s) 9, 770
BsmBI CGTCTC 1 cut(s) 9
BsmFI GGGAC 2 cut(s) 262, 347
BsnI GGCC 3 cut(s) 143, 242, 591
Bso31I GGTCTC 1 cut(s) 770
BsoBI CYCGRG 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 462
Bsp143I GATC 4 cut(s) 247, 255, 342, 730
BspACI CCGC 9 cut(s) 12, 154, 166, 376, 378, 506, 588, 612, 726
BspANI GGCC 3 cut(s) 143, 242, 591
BspCNI CTCAG 2 cut(s) 660, 763
BspFNI CGCG 3 cut(s) 156, 378, 506
BspLI GGNNCC 2 cut(s) 61, 592
BspMI ACCTGC 1 cut(s) 536
BspPI GGATC 1 cut(s) 725
BspQI GCTCTTC 1 cut(s) 628
BspT107I GGYRCC 1 cut(s) 59
BspTNI GGTCTC 1 cut(s) 770
BsrDI GCAATG 1 cut(s) 717
BsrFI RCCGGY 2 cut(s) 56, 722
BssAI RCCGGY 2 cut(s) 56, 722
BssECI CCNNGG 6 cut(s) 85, 237, 281, 376, 759, 790
BssMI GATC 4 cut(s) 247, 255, 342, 730
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 1 cut(s) 761
Bst4CI ACNGT 1 cut(s) 700
Bst6I CTCTTC 1 cut(s) 628
BstACI GRCGYC 1 cut(s) 60
BstC8I GCNNGC 5 cut(s) 58, 205, 394, 458, 724
BstDEI CTNAG 3 cut(s) 668, 750, 767
BstDSI CCRYGG 2 cut(s) 237, 376
BstF5I GGATG 2 cut(s) 36, 526
BstFNI CGCG 3 cut(s) 156, 378, 506
BstH2I RGCGCY 1 cut(s) 63
BstHHI GCGC 1 cut(s) 62
BstKTI GATC 4 cut(s) 250, 258, 345, 733
BstMAI GTCTC 2 cut(s) 9, 770
BstMBI GATC 4 cut(s) 247, 255, 342, 730
BstMCI CGRYCG 1 cut(s) 345
BstMWI GCNNNNNNNGC 7 cut(s) 213, 219, 384, 393, 398, 618, 627
BstNI CCWGG 1 cut(s) 761
BstSCI CCNGG 2 cut(s) 222, 759
BstUI CGCG 3 cut(s) 156, 378, 506
BstV1I GCAGC 4 cut(s) 200, 627, 630, 727
Bsu36I CCTNAGG 1 cut(s) 767
BsuRI GGCC 3 cut(s) 143, 242, 591
BtgI CCRYGG 2 cut(s) 237, 376
BtsCI GGATG 2 cut(s) 36, 526
BveI ACCTGC 1 cut(s) 536
Cac8I GCNNGC 5 cut(s) 58, 205, 394, 458, 724
CfoI GCGC 1 cut(s) 62
Cfr10I RCCGGY 2 cut(s) 56, 722
Cfr13I GGNCC 2 cut(s) 590, 763
Cfr42I CCGCGG 1 cut(s) 379
CseI GACGC 2 cut(s) 205, 376
CviAII CATG 2 cut(s) 448, 627
DdeI CTNAG 3 cut(s) 668, 750, 767
DinI GGCGCC 1 cut(s) 61
DpnI GATC 4 cut(s) 249, 257, 344, 732
DpnII GATC 4 cut(s) 247, 255, 342, 730
Eam1104I CTCTTC 1 cut(s) 628
EarI CTCTTC 1 cut(s) 628
EciI GGCGGA 1 cut(s) 155
Eco130I CCWWGG 1 cut(s) 281
Eco31I GGTCTC 1 cut(s) 770
Eco47I GGWCC 1 cut(s) 763
Eco57I CTGAAG 1 cut(s) 311
Eco81I CCTNAGG 1 cut(s) 767
Eco88I CYCGRG 1 cut(s) 474
EcoO109I RGGNCCY 1 cut(s) 763
EcoRI GAATTC 1 cut(s) 232
EcoRII CCWGG 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 281
EgeI GGCGCC 1 cut(s) 61
EheI GGCGCC 1 cut(s) 61
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 9
FaeI CATG 2 cut(s) 451, 630
FaiI YATR 7 cut(s) 116, 431, 449, 469, 534, 552, 628
FaqI GGGAC 2 cut(s) 262, 347
FatI CATG 2 cut(s) 447, 626
FokI GGATG 2 cut(s) 23, 533
GlaI GCGC 1 cut(s) 61
GsuI CTGGAG 2 cut(s) 193, 349
HaeII RGCGCY 1 cut(s) 63
HaeIII GGCC 3 cut(s) 143, 242, 591
HapII CCGG 4 cut(s) 57, 223, 367, 723
HgaI GACGC 2 cut(s) 205, 376
HhaI GCGC 1 cut(s) 62
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 2 cut(s) 451, 630
Hin6I GCGC 1 cut(s) 60
HinP1I GCGC 1 cut(s) 60
HindIII AAGCTT 1 cut(s) 322
HinfI GANTC 5 cut(s) 101, 190, 286, 490, 662
HpaII CCGG 4 cut(s) 57, 223, 367, 723
HphI GGTGA 3 cut(s) 310, 433, 779
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 6 cut(s) 100, 247, 291, 347, 495, 520
Hpy188III TCNNGA 7 cut(s) 178, 187, 194, 229, 251, 328, 476
Hpy8I GTNNAC 1 cut(s) 703
Hpy99I CGWCG 4 cut(s) 32, 35, 263, 392
HpyAV CCTTC 3 cut(s) 280, 286, 578
HpyCH4III ACNGT 1 cut(s) 700
HpyCH4V TGCA 5 cut(s) 524, 554, 640, 682, 695
HpyF10VI GCNNNNNNNGC 7 cut(s) 213, 219, 384, 393, 398, 618, 627
HpyF3I CTNAG 3 cut(s) 668, 750, 767
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 2 cut(s) 451, 630
HspAI GCGC 1 cut(s) 60
KasI GGCGCC 1 cut(s) 59
KroI GCCGGC 2 cut(s) 56, 722
KroNI GCCGGC 2 cut(s) 58, 724
KspI CCGCGG 1 cut(s) 379
Kzo9I GATC 4 cut(s) 247, 255, 342, 730
LguI GCTCTTC 1 cut(s) 628
LmnI GCTCC 2 cut(s) 189, 212
Lsp1109I GCAGC 4 cut(s) 200, 627, 630, 727
LweI GCATC 3 cut(s) 501, 511, 682
MalI GATC 4 cut(s) 249, 257, 344, 732
MboI GATC 4 cut(s) 247, 255, 342, 730
MboII GAAGA 3 cut(s) 32, 166, 615
MhlI GDGCHC 1 cut(s) 462
MluCI AATT 3 cut(s) 232, 337, 350
Mly113I GGCGCC 1 cut(s) 60
MlyI GAGTC 1 cut(s) 95
MmeI TCCRAC 1 cut(s) 123
MreI CGCCGGCG 1 cut(s) 56
MroNI GCCGGC 2 cut(s) 56, 722
MseI TTAA 1 cut(s) 320
MspA1I CMGCKG 2 cut(s) 213, 378
MspI CCGG 4 cut(s) 57, 223, 367, 723
MspR9I CCNGG 2 cut(s) 224, 761
MvaI CCWGG 1 cut(s) 761
MvnI CGCG 3 cut(s) 156, 378, 506
MwoI GCNNNNNNNGC 7 cut(s) 213, 219, 384, 393, 398, 618, 627
NaeI GCCGGC 2 cut(s) 58, 724
NarI GGCGCC 1 cut(s) 60
NciI CCSGG 1 cut(s) 224
NdeII GATC 4 cut(s) 247, 255, 342, 730
NgoMIV GCCGGC 2 cut(s) 56, 722
NlaIII CATG 2 cut(s) 451, 630
NlaIV GGNNCC 2 cut(s) 61, 592
NmeAIII GCCGAG 3 cut(s) 66, 119, 771
PciSI GCTCTTC 1 cut(s) 628
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 60
PdiI GCCGGC 2 cut(s) 58, 724
PfeI GAWTC 4 cut(s) 190, 286, 490, 662
PflFI GACNNNGTC 1 cut(s) 386
Ple19I CGATCG 1 cut(s) 345
PleI GAGTC 1 cut(s) 95
PluTI GGCGCC 1 cut(s) 63
PpsI GAGTC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 763
Psp5II RGGWCCY 1 cut(s) 763
Psp6I CCWGG 1 cut(s) 759
PspGI CCWGG 1 cut(s) 759
PspN4I GGNNCC 2 cut(s) 61, 592
PspPI GGNCC 2 cut(s) 590, 763
PspPPI RGGWCCY 1 cut(s) 763
PsyI GACNNNGTC 1 cut(s) 386
PvuI CGATCG 1 cut(s) 345
PvuII CAGCTG 1 cut(s) 213
SacII CCGCGG 1 cut(s) 379
SapI GCTCTTC 1 cut(s) 628
SaqAI TTAA 1 cut(s) 320
Sau3AI GATC 4 cut(s) 247, 255, 342, 730
Sau96I GGNCC 2 cut(s) 590, 763
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 224, 761
SduI GDGCHC 1 cut(s) 462
SfaNI GCATC 3 cut(s) 501, 511, 682
SfoI GGCGCC 1 cut(s) 61
Sfr303I CCGCGG 1 cut(s) 379
SgrAI CRCCGGYG 1 cut(s) 56
SgrBI CCGCGG 1 cut(s) 379
SinI GGWCC 1 cut(s) 763
Sse9I AATT 3 cut(s) 232, 337, 350
SsiI CCGC 9 cut(s) 12, 154, 166, 376, 378, 506, 588, 612, 726
SspDI GGCGCC 1 cut(s) 59
StyD4I CCNGG 2 cut(s) 222, 759
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 1 cut(s) 700
TaqI TCGA 3 cut(s) 177, 261, 341
TasI AATT 3 cut(s) 232, 337, 350
TauI GCSGC 5 cut(s) 156, 381, 591, 615, 729
TfiI GAWTC 4 cut(s) 190, 286, 490, 662
Tru1I TTAA 1 cut(s) 320
Tru9I TTAA 1 cut(s) 320
TseI GCWGC 5 cut(s) 9, 213, 615, 618, 740
TspDTI ATGAA 2 cut(s) 140, 615
TspGWI ACGGA 3 cut(s) 226, 403, 477
Tth111I GACNNNGTC 1 cut(s) 386
VpaK11BI GGWCC 1 cut(s) 763
XapI RAATTY 2 cut(s) 232, 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.