pycom04g18100

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013410
Physical Location & Seq
Reverse (-)
20261413 .. 20262514
1102 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g18100.1

Sequence Viewer

Length: 882 bp
ATGGCAGAAGCTGGTTCTGTTTTATTGACCGAAACTAATTTGAACTCTCTCCCCCAAACGACAATGGGTTGCTGCGGAGACGAAGAAAACGACGACGACATCCACAATCCTCCCAACGCCGGCGCCGACACCACCTCCACCCAAACCCTCGGCGAATACTCCGACTCTACCACCACCATATCCCCGATGAACTCTCACTTCCCGGCCTTGACTTGCCGCGACACCCTCCGCCTCATCTTCGAGAAGCTCCCGATTCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGAGTCTGGAACTCCGTCGCCTCCGATCGATCTAAATATGACAAGGAGATCGTCGATAAAGCCTTCAAGTCCCCTTGGAATCTGAAGGAGGTGCTTGGAAAGCCCTCGTCTTTAAGCTTCTGGAGGGACAATTCGATCGGAAAATTCGCCATCTCCCACCGGATTGTCCGCGGCGACAGCGTCGCCAGCCTCGCCGTCAAGTATTCCGTCCAGGTGATGGACATAAAACGATTGAACAACATGATGAGCGAGCACGGAATATACTCTCGGGAGAGATTGCTGATTCCGATAAGCAACGCGGAGATGCTTTCGGAGGCAACCTGCTATGTAGAAGTGGATAATTATGCAAAGAGAGAAGTGGCTGTGTTGTATTTGGAAGGCGGCCCCGACCCGAAAACCATAGGCAGCAGCAGTTCTTCATGCTCTGTCTTGCAGACCAAGAAAAGAGTGGTTGAATCACTGAGGAGAAGTCTGCAAGTAGATGATGCAACTGTGAACTACTACTTGTCCATTGCCGGCGGGGATCCAAGAGCTGCAATCTCTCAGTTCTCCCAGGACCTTAGGTGGGAGACCCACACTCACCCCGGCTTTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

294

Amino Acids

32.37

Weight (kDa)

5.58

Isoelectric Point (pI)

42.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 73 - 108 1.1e-06 F-box domain
F-box-like PF12937 76 - 105 1e-06 F-box-like
LysM PF01476 148 - 191 2.4e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 122
AccB7I CCANNNNNTGG 1 cut(s) 502
AccII CGCG 3 cut(s) 219, 456, 584
AciI CCGC 8 cut(s) 75, 217, 229, 454, 456, 584, 666, 804
AclWI GGATC 2 cut(s) 803, 816
AcsI RAATTY 1 cut(s) 428
AcuI CTGAAG 1 cut(s) 389
AcyI GRCGYC 1 cut(s) 123
AfiI CCNNNNNNNGG 3 cut(s) 119, 502, 850
AgsI TTSAA 4 cut(s) 43, 352, 520, 740
AjnI CCWGG 2 cut(s) 495, 837
AluBI AGCT 5 cut(s) 11, 247, 276, 402, 818
AluI AGCT 5 cut(s) 11, 247, 276, 402, 818
Alw21I GWGCWC 1 cut(s) 540
Alw26I GTCTC 2 cut(s) 72, 848
AlwI GGATC 2 cut(s) 803, 816
AlwNI CAGNNNCTG 1 cut(s) 11
Ama87I CYCGRG 1 cut(s) 552
AoxI GGCC 2 cut(s) 204, 667
ApeKI GCWGC 5 cut(s) 72, 276, 690, 693, 818
ApoI RAATTY 1 cut(s) 428
AspLEI GCGC 1 cut(s) 125
AspS9I GGNCC 2 cut(s) 668, 841
AsuC2I CCSGG 2 cut(s) 203, 870
AsuHPI GGTGA 2 cut(s) 511, 857
AvaI CYCGRG 1 cut(s) 552
AvaII GGWCC 1 cut(s) 841
AxyI CCTNAGG 1 cut(s) 845
BamHI GGATCC 1 cut(s) 808
BanI GGYRCC 1 cut(s) 122
Bbv12I GWGCWC 1 cut(s) 540
BbvI GCAGC 5 cut(s) 59, 263, 702, 705, 805
BccI CCATC 2 cut(s) 443, 496
BceAI ACGGC 1 cut(s) 464
BciT130I CCWGG 2 cut(s) 497, 839
BcnI CCSGG 2 cut(s) 203, 870
BcoDI GTCTC 2 cut(s) 72, 848
BfoI RGCGCY 1 cut(s) 126
BfuAI ACCTGC 1 cut(s) 614
BisI GCNGC 8 cut(s) 73, 217, 277, 457, 667, 691, 694, 819
BlsI GCNGC 8 cut(s) 74, 218, 278, 458, 668, 692, 695, 820
Bme1390I CCNGG 4 cut(s) 203, 497, 839, 870
Bme18I GGWCC 1 cut(s) 841
BmeT110I CYCGRG 1 cut(s) 552
BmgT120I GGNCC 2 cut(s) 668, 841
BmiI GGNNCC 3 cut(s) 124, 670, 810
BmrFI CCNGG 4 cut(s) 203, 497, 839, 870
BmsI GCATC 2 cut(s) 579, 760
BpmI CTGGAG 2 cut(s) 256, 427
BpuMI CCSGG 2 cut(s) 203, 870
Bsa29I ATCGAT 1 cut(s) 313
BsaHI GRCGYC 1 cut(s) 123
BsaI GGTCTC 1 cut(s) 848
BsaJI CCNNGG 5 cut(s) 148, 359, 454, 837, 868
BsaWI WCCGGW 1 cut(s) 444
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 502, 850
Bse118I RCCGGY 2 cut(s) 119, 800
Bse21I CCTNAGG 1 cut(s) 845
Bse3DI GCAATG 1 cut(s) 795
BseBI CCWGG 2 cut(s) 497, 839
BseCI ATCGAT 1 cut(s) 313
BseDI CCNNGG 5 cut(s) 148, 359, 454, 837, 868
BseGI GGATG 1 cut(s) 99
BseLI CCNNNNNNNGG 3 cut(s) 119, 502, 850
BseMI GCAATG 1 cut(s) 795
BseMII CTCAG 2 cut(s) 737, 842
BseRI GAGGAG 1 cut(s) 763
BseXI GCAGC 5 cut(s) 59, 263, 702, 705, 805
Bsh1236I CGCG 3 cut(s) 219, 456, 584
Bsh1285I CGRYCG 2 cut(s) 313, 423
BshFI GGCC 2 cut(s) 206, 669
BshNI GGYRCC 1 cut(s) 122
BshVI ATCGAT 1 cut(s) 313
BsiEI CGRYCG 2 cut(s) 313, 423
BsiHKAI GWGCWC 1 cut(s) 540
BsiHKCI CYCGRG 1 cut(s) 552
BsiSI CCGG 5 cut(s) 120, 203, 445, 801, 870
BslFI GGGAC 2 cut(s) 340, 425
BslI CCNNNNNNNGG 3 cut(s) 119, 502, 850
BsmAI GTCTC 2 cut(s) 72, 848
BsmBI CGTCTC 1 cut(s) 72
BsmFI GGGAC 2 cut(s) 340, 425
BsnI GGCC 2 cut(s) 206, 669
Bso31I GGTCTC 1 cut(s) 848
BsoBI CYCGRG 1 cut(s) 552
Bsp1286I GDGCHC 1 cut(s) 540
Bsp143I GATC 5 cut(s) 310, 314, 333, 420, 808
BspACI CCGC 8 cut(s) 75, 217, 229, 454, 456, 584, 666, 804
BspANI GGCC 2 cut(s) 206, 669
BspCNI CTCAG 2 cut(s) 738, 841
BspDI ATCGAT 1 cut(s) 313
BspFNI CGCG 3 cut(s) 219, 456, 584
BspLI GGNNCC 3 cut(s) 124, 670, 810
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 2 cut(s) 803, 816
BspT107I GGYRCC 1 cut(s) 122
BspTNI GGTCTC 1 cut(s) 848
BsrDI GCAATG 1 cut(s) 795
BsrFI RCCGGY 2 cut(s) 119, 800
BssAI RCCGGY 2 cut(s) 119, 800
BssECI CCNNGG 5 cut(s) 148, 359, 454, 837, 868
BssMI GATC 5 cut(s) 310, 314, 333, 420, 808
BssNI GRCGYC 1 cut(s) 123
BssT1I CCWWGG 1 cut(s) 359
Bst2UI CCWGG 2 cut(s) 497, 839
Bst4CI ACNGT 1 cut(s) 778
BstACI GRCGYC 1 cut(s) 123
BstC8I GCNNGC 5 cut(s) 121, 268, 472, 536, 802
BstDEI CTNAG 4 cut(s) 746, 828, 845, 879
BstDSI CCRYGG 1 cut(s) 454
BstF5I GGATG 1 cut(s) 99
BstFNI CGCG 3 cut(s) 219, 456, 584
BstH2I RGCGCY 1 cut(s) 126
BstHHI GCGC 1 cut(s) 125
BstKTI GATC 5 cut(s) 313, 317, 336, 423, 811
BstMAI GTCTC 2 cut(s) 72, 848
BstMBI GATC 5 cut(s) 310, 314, 333, 420, 808
BstMCI CGRYCG 2 cut(s) 313, 423
BstMWI GCNNNNNNNGC 6 cut(s) 276, 282, 385, 462, 471, 476
BstNI CCWGG 2 cut(s) 497, 839
BstSCI CCNGG 4 cut(s) 201, 495, 837, 868
BstUI CGCG 3 cut(s) 219, 456, 584
BstV1I GCAGC 5 cut(s) 59, 263, 702, 705, 805
BstX2I RGATCY 1 cut(s) 808
BstYI RGATCY 1 cut(s) 808
Bsu15I ATCGAT 1 cut(s) 313
Bsu36I CCTNAGG 1 cut(s) 845
BsuRI GGCC 2 cut(s) 206, 669
BsuTUI ATCGAT 1 cut(s) 313
BtgI CCRYGG 1 cut(s) 454
BtsCI GGATG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 743
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 5 cut(s) 121, 268, 472, 536, 802
CaiI CAGNNNCTG 1 cut(s) 11
CfoI GCGC 1 cut(s) 125
Cfr10I RCCGGY 2 cut(s) 119, 800
Cfr13I GGNCC 2 cut(s) 668, 841
Cfr42I CCGCGG 1 cut(s) 457
ClaI ATCGAT 1 cut(s) 313
CseI GACGC 2 cut(s) 268, 454
CviAII CATG 2 cut(s) 526, 705
DdeI CTNAG 4 cut(s) 746, 828, 845, 879
DinI GGCGCC 1 cut(s) 124
DpnI GATC 5 cut(s) 312, 316, 335, 422, 810
DpnII GATC 5 cut(s) 310, 314, 333, 420, 808
EciI GGCGGA 1 cut(s) 218
Eco130I CCWWGG 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 848
Eco47I GGWCC 1 cut(s) 841
Eco57I CTGAAG 1 cut(s) 389
Eco81I CCTNAGG 1 cut(s) 845
Eco88I CYCGRG 1 cut(s) 552
EcoO109I RGGNCCY 1 cut(s) 841
EcoRII CCWGG 2 cut(s) 495, 837
EcoT14I CCWWGG 1 cut(s) 359
EgeI GGCGCC 1 cut(s) 124
EheI GGCGCC 1 cut(s) 124
ErhI CCWWGG 1 cut(s) 359
Esp3I CGTCTC 1 cut(s) 72
FaeI CATG 2 cut(s) 529, 708
FaiI YATR 9 cut(s) 179, 324, 509, 527, 547, 612, 630, 686, 706
FaqI GGGAC 2 cut(s) 340, 425
FatI CATG 2 cut(s) 525, 704
FauI CCCGC 1 cut(s) 797
Fnu4HI GCNGC 8 cut(s) 73, 217, 277, 457, 667, 691, 694, 819
FokI GGATG 1 cut(s) 86
Fsp4HI GCNGC 8 cut(s) 73, 217, 277, 457, 667, 691, 694, 819
GlaI GCGC 1 cut(s) 124
GluI GCNGC 8 cut(s) 73, 217, 277, 457, 667, 691, 694, 819
GsuI CTGGAG 2 cut(s) 256, 427
HaeII RGCGCY 1 cut(s) 126
HaeIII GGCC 2 cut(s) 206, 669
HapII CCGG 5 cut(s) 120, 203, 445, 801, 870
HgaI GACGC 2 cut(s) 268, 454
HhaI GCGC 1 cut(s) 125
Hin1I GRCGYC 1 cut(s) 123
Hin1II CATG 2 cut(s) 529, 708
Hin6I GCGC 1 cut(s) 123
HinP1I GCGC 1 cut(s) 123
HindIII AAGCTT 1 cut(s) 400
HinfI GANTC 6 cut(s) 164, 253, 288, 364, 568, 740
HpaII CCGG 5 cut(s) 120, 203, 445, 801, 870
HphI GGTGA 2 cut(s) 511, 857
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 6 cut(s) 163, 310, 369, 425, 573, 598
Hpy188III TCNNGA 6 cut(s) 241, 250, 257, 292, 406, 554
Hpy8I GTNNAC 1 cut(s) 781
Hpy99I CGWCG 5 cut(s) 95, 98, 305, 341, 470
HpyAV CCTTC 3 cut(s) 358, 364, 656
HpyCH4III ACNGT 1 cut(s) 778
HpyCH4V TGCA 5 cut(s) 632, 718, 760, 773, 821
HpyF10VI GCNNNNNNNGC 6 cut(s) 276, 282, 385, 462, 471, 476
HpyF3I CTNAG 4 cut(s) 746, 828, 845, 879
Hsp92I GRCGYC 1 cut(s) 123
Hsp92II CATG 2 cut(s) 529, 708
HspAI GCGC 1 cut(s) 123
KasI GGCGCC 1 cut(s) 122
KroI GCCGGC 2 cut(s) 119, 800
KroNI GCCGGC 2 cut(s) 121, 802
KspI CCGCGG 1 cut(s) 457
Kzo9I GATC 5 cut(s) 310, 314, 333, 420, 808
LmnI GCTCC 2 cut(s) 252, 275
Lsp1109I GCAGC 5 cut(s) 59, 263, 702, 705, 805
LweI GCATC 2 cut(s) 579, 760
MalI GATC 5 cut(s) 312, 316, 335, 422, 810
MboI GATC 5 cut(s) 310, 314, 333, 420, 808
MboII GAAGA 3 cut(s) 95, 229, 693
MflI RGATCY 1 cut(s) 808
MhlI GDGCHC 1 cut(s) 540
MluCI AATT 4 cut(s) 37, 415, 428, 625
Mly113I GGCGCC 1 cut(s) 123
MlyI GAGTC 2 cut(s) 158, 297
MmeI TCCRAC 1 cut(s) 186
MreI CGCCGGCG 1 cut(s) 119
MroNI GCCGGC 2 cut(s) 119, 800
MseI TTAA 1 cut(s) 398
MspA1I CMGCKG 2 cut(s) 276, 456
MspI CCGG 5 cut(s) 120, 203, 445, 801, 870
MspR9I CCNGG 4 cut(s) 203, 497, 839, 870
MvaI CCWGG 2 cut(s) 497, 839
MvnI CGCG 3 cut(s) 219, 456, 584
MwoI GCNNNNNNNGC 6 cut(s) 276, 282, 385, 462, 471, 476
NaeI GCCGGC 2 cut(s) 121, 802
NarI GGCGCC 1 cut(s) 123
NciI CCSGG 2 cut(s) 203, 870
NdeII GATC 5 cut(s) 310, 314, 333, 420, 808
NgoMIV GCCGGC 2 cut(s) 119, 800
NlaIII CATG 2 cut(s) 529, 708
NlaIV GGNNCC 3 cut(s) 124, 670, 810
NmeAIII GCCGAG 2 cut(s) 129, 311
PcsI WCGNNNNNNNCGW 2 cut(s) 87, 123
PdiI GCCGGC 2 cut(s) 121, 802
PfeI GAWTC 4 cut(s) 253, 364, 568, 740
PflFI GACNNNGTC 1 cut(s) 464
PflMI CCANNNNNTGG 1 cut(s) 502
PkrI GCNGC 8 cut(s) 74, 218, 278, 458, 668, 692, 695, 820
Ple19I CGATCG 2 cut(s) 313, 423
PleI GAGTC 2 cut(s) 158, 296
PluTI GGCGCC 1 cut(s) 126
PpsI GAGTC 2 cut(s) 158, 296
PpuMI RGGWCCY 1 cut(s) 841
Psp5II RGGWCCY 1 cut(s) 841
Psp6I CCWGG 2 cut(s) 495, 837
PspGI CCWGG 2 cut(s) 495, 837
PspN4I GGNNCC 3 cut(s) 124, 670, 810
PspPI GGNCC 2 cut(s) 668, 841
PspPPI RGGWCCY 1 cut(s) 841
PstNI CAGNNNCTG 1 cut(s) 11
PsuI RGATCY 1 cut(s) 808
PsyI GACNNNGTC 1 cut(s) 464
PvuI CGATCG 2 cut(s) 313, 423
PvuII CAGCTG 1 cut(s) 276
SacII CCGCGG 1 cut(s) 457
SaqAI TTAA 1 cut(s) 398
SatI GCNGC 8 cut(s) 73, 217, 277, 457, 667, 691, 694, 819
Sau3AI GATC 5 cut(s) 310, 314, 333, 420, 808
Sau96I GGNCC 2 cut(s) 668, 841
SchI GAGTC 2 cut(s) 158, 297
ScrFI CCNGG 4 cut(s) 203, 497, 839, 870
SduI GDGCHC 1 cut(s) 540
SfaNI GCATC 2 cut(s) 579, 760
SfoI GGCGCC 1 cut(s) 124
Sfr303I CCGCGG 1 cut(s) 457
SgrAI CRCCGGYG 1 cut(s) 119
SgrBI CCGCGG 1 cut(s) 457
SinI GGWCC 1 cut(s) 841
Sse9I AATT 4 cut(s) 37, 415, 428, 625
SsiI CCGC 8 cut(s) 75, 217, 229, 454, 456, 584, 666, 804
SspDI GGCGCC 1 cut(s) 122
StyD4I CCNGG 4 cut(s) 201, 495, 837, 868
StyI CCWWGG 1 cut(s) 359
TaaI ACNGT 1 cut(s) 778
TaqI TCGA 4 cut(s) 240, 313, 339, 419
TaqII GACCGA 1 cut(s) 44
TasI AATT 4 cut(s) 37, 415, 428, 625
TauI GCSGC 3 cut(s) 219, 459, 669
TfiI GAWTC 4 cut(s) 253, 364, 568, 740
Tru1I TTAA 1 cut(s) 398
Tru9I TTAA 1 cut(s) 398
TscAI CASTG 1 cut(s) 750
TseI GCWGC 5 cut(s) 72, 276, 690, 693, 818
TspDTI ATGAA 2 cut(s) 203, 693
TspGWI ACGGA 3 cut(s) 289, 481, 555
TspRI CASTG 1 cut(s) 750
Tth111I GACNNNGTC 1 cut(s) 464
Van91I CCANNNNNTGG 1 cut(s) 502
VpaK11BI GGWCC 1 cut(s) 841
XapI RAATTY 1 cut(s) 428
XcmI CCANNNNNNNNNTGG 1 cut(s) 730
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.