Rh3BG065300

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0013410
Physical Location & Seq
Forward (+)
4366090 .. 4367355
1266 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG065300.1

Sequence Viewer

Length: 831 bp
ATGGGTTGTTGCGGCGACGAAGAAAACGACGACGAAATCCTCAAACCTCTCAGCCCCACCTCCTCCACCACCACCCAAACCCTAGACGACTCGTCATCCTCCTCCTCCGACGGCTACACCACCATATCCCCGATGAACTCTCACTTCTCGGCCCTGGCCTGCCGCGACACCCTCCGCCTCATCTTCGAGAAGCTTCCGATCCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGCGCCTGGAACTCCGTCGCTTCCGATCAAGAGATCGTCGCCAACGCCTTCAAATCGCCGTGGAAGCTCAAGGACGTCGTCGGAAAACCCGCCTCCGGAAGCTTCTGGAGAGATAACAGCCTCGGGAAATTCGCCATCTCACACCGAATTGCCCGCGGCGATAGCGTCGCTAGCCTCGCCGTCAAGTTTTCCGTTCAGGTGATGGACATAAAGCGCTTGAACAACATGATGAGCGAGCATGGAATCTACTCGAGGGAGAGGTTGTTGATTCCGATAAGCGATCCTGAAATGCTTGTGGGAGCTACCTGCTATGTCGAAGTGGATCCCTGTGCCAAGAGAGAAGTTGCGGTGTTGTATTTGGAAGATGGTCCTGACCAGAAATCAATGAGCTCTTGCTCTTTGTCGCAGAAGAGTGCCGACAGCAGTGGCTTGAATTCTGAACTAGGTAAGAAGAGGGTTCTTGAGTCTCTGAGGAGAAGCATGCACGTTGATGATGCCACTGCTCTGTACTACTTGTCCATTGCTGATGGGGATCCGAGAGCTGCATTGTCTCAGTTCTCGCAGGACCTTAGGTGGGAGACTCATTCGGCCTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

276

Amino Acids

30.23

Weight (kDa)

5.31

Isoelectric Point (pI)

46.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 55 - 89 1.8e-07 F-box domain
F-box-like PF12937 58 - 88 1.8e-07 F-box-like
LysM PF01476 125 - 168 7.6e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 309
Acc36I ACCTGC 1 cut(s) 545
AccII CGCG 3 cut(s) 165, 234, 387
AccIII TCCGGA 1 cut(s) 326
AciI CCGC 8 cut(s) 12, 163, 175, 232, 321, 385, 387, 578
AclWI GGATC 6 cut(s) 193, 506, 548, 561, 758, 771
AcsI RAATTY 2 cut(s) 359, 664
AcyI GRCGYC 1 cut(s) 306
AfaI GTAC 1 cut(s) 740
AfeI AGCGCT 1 cut(s) 446
AfiI CCNNNNNNNGG 2 cut(s) 326, 805
AgsI TTSAA 3 cut(s) 283, 451, 664
AhdI GACNNNNNGTC 1 cut(s) 91
AjnI CCWGG 2 cut(s) 153, 236
AluBI AGCT 7 cut(s) 193, 222, 298, 333, 533, 621, 773
AluI AGCT 7 cut(s) 193, 222, 298, 333, 533, 621, 773
Alw21I GWGCWC 1 cut(s) 623
Alw26I GTCTC 3 cut(s) 702, 786, 803
AlwI GGATC 6 cut(s) 193, 506, 548, 561, 758, 771
Ama87I CYCGRG 2 cut(s) 353, 481
Aor13HI TCCGGA 1 cut(s) 326
Aor51HI AGCGCT 1 cut(s) 446
AoxI GGCC 3 cut(s) 150, 156, 819
ApeKI GCWGC 2 cut(s) 222, 773
ApoI RAATTY 2 cut(s) 359, 664
AspLEI GCGC 2 cut(s) 236, 447
AspS9I GGNCC 3 cut(s) 151, 599, 796
AsuHPI GGTGA 1 cut(s) 442
AsuNHI GCTAGC 1 cut(s) 401
AvaI CYCGRG 2 cut(s) 353, 481
AvaII GGWCC 2 cut(s) 599, 796
AxyI CCTNAGG 1 cut(s) 800
BamHI GGATCC 2 cut(s) 553, 763
BanII GRGCYC 1 cut(s) 623
Bbv12I GWGCWC 1 cut(s) 623
BbvI GCAGC 2 cut(s) 209, 760
BccI CCATC 4 cut(s) 374, 427, 590, 752
BceAI ACGGC 3 cut(s) 127, 274, 395
BciT130I CCWGG 2 cut(s) 155, 238
BcoDI GTCTC 3 cut(s) 702, 786, 803
BfaI CTAG 3 cut(s) 83, 402, 674
BfoI RGCGCY 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 545
BisI GCNGC 6 cut(s) 13, 163, 223, 232, 388, 774
BlsI GCNGC 6 cut(s) 14, 164, 224, 233, 389, 775
Bme1390I CCNGG 2 cut(s) 155, 238
Bme18I GGWCC 2 cut(s) 599, 796
BmeRI GACNNNNNGTC 1 cut(s) 91
BmeT110I CYCGRG 2 cut(s) 353, 481
BmgT120I GGNCC 3 cut(s) 151, 599, 796
BmiI GGNNCC 2 cut(s) 555, 765
BmrFI CCNGG 2 cut(s) 155, 238
BmsI GCATC 1 cut(s) 715
BmtI GCTAGC 1 cut(s) 405
BpmI CTGGAG 2 cut(s) 202, 358
BpuEI CTTGAG 2 cut(s) 284, 713
BsaBI GATNNNNATC 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 306
BsaJI CCNNGG 4 cut(s) 153, 290, 352, 385
BsaWI WCCGGW 1 cut(s) 326
BsaXI ACNNNNNCTCC 2 cut(s) 479, 509
Bsc4I CCNNNNNNNGG 2 cut(s) 326, 805
Bse21I CCTNAGG 1 cut(s) 800
Bse3DI GCAATG 1 cut(s) 750
Bse8I GATNNNNATC 1 cut(s) 762
BseAI TCCGGA 1 cut(s) 326
BseBI CCWGG 2 cut(s) 155, 238
BseDI CCNNGG 4 cut(s) 153, 290, 352, 385
BseGI GGATG 1 cut(s) 95
BseJI GATNNNNATC 1 cut(s) 762
BseLI CCNNNNNNNGG 2 cut(s) 326, 805
BseMI GCAATG 1 cut(s) 750
BseMII CTCAG 3 cut(s) 64, 692, 797
BseRI GAGGAG 4 cut(s) 52, 91, 94, 718
BseXI GCAGC 2 cut(s) 209, 760
Bsh1236I CGCG 3 cut(s) 165, 234, 387
BshFI GGCC 3 cut(s) 152, 158, 821
BsiHKAI GWGCWC 1 cut(s) 623
BsiHKCI CYCGRG 2 cut(s) 353, 481
BsiSI CCGG 1 cut(s) 327
BslI CCNNNNNNNGG 2 cut(s) 326, 805
BsmAI GTCTC 3 cut(s) 702, 786, 803
BsnI GGCC 3 cut(s) 152, 158, 821
BsoBI CYCGRG 2 cut(s) 353, 481
Bsp1286I GDGCHC 1 cut(s) 623
Bsp13I TCCGGA 1 cut(s) 326
Bsp143I GATC 6 cut(s) 198, 256, 264, 511, 553, 763
BspACI CCGC 8 cut(s) 12, 163, 175, 232, 321, 385, 387, 578
BspANI GGCC 3 cut(s) 152, 158, 821
BspCNI CTCAG 3 cut(s) 63, 693, 796
BspEI TCCGGA 1 cut(s) 326
BspFNI CGCG 3 cut(s) 165, 234, 387
BspLI GGNNCC 2 cut(s) 555, 765
BspMI ACCTGC 1 cut(s) 545
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 6 cut(s) 193, 506, 548, 561, 758, 771
BsrDI GCAATG 1 cut(s) 750
BssECI CCNNGG 4 cut(s) 153, 290, 352, 385
BssMI GATC 6 cut(s) 198, 256, 264, 511, 553, 763
BssNI GRCGYC 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 155, 238
Bst6I CTCTTC 2 cut(s) 635, 677
BstACI GRCGYC 1 cut(s) 306
BstC8I GCNNGC 6 cut(s) 160, 214, 385, 403, 467, 713
BstDEI CTNAG 4 cut(s) 50, 701, 783, 800
BstDSI CCRYGG 2 cut(s) 290, 385
BstF5I GGATG 1 cut(s) 95
BstFNI CGCG 3 cut(s) 165, 234, 387
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 2 cut(s) 236, 447
BstKTI GATC 6 cut(s) 201, 259, 267, 514, 556, 766
BstMAI GTCTC 3 cut(s) 702, 786, 803
BstMBI GATC 6 cut(s) 198, 256, 264, 511, 553, 763
BstMWI GCNNNNNNNGC 7 cut(s) 222, 228, 231, 295, 393, 402, 407
BstNI CCWGG 2 cut(s) 155, 238
BstNSI RCATGY 1 cut(s) 715
BstSCI CCNGG 2 cut(s) 153, 236
BstUI CGCG 3 cut(s) 165, 234, 387
BstV1I GCAGC 2 cut(s) 209, 760
BstX2I RGATCY 2 cut(s) 553, 763
BstYI RGATCY 2 cut(s) 553, 763
Bsu36I CCTNAGG 1 cut(s) 800
BsuRI GGCC 3 cut(s) 152, 158, 821
BtgI CCRYGG 2 cut(s) 290, 385
BtsCI GGATG 1 cut(s) 95
BtsI GCAGTG 2 cut(s) 661, 729
BtsIMutI CAGTG 2 cut(s) 661, 729
BveI ACCTGC 1 cut(s) 545
Cac8I GCNNGC 6 cut(s) 160, 214, 385, 403, 467, 713
CfoI GCGC 2 cut(s) 236, 447
Cfr13I GGNCC 3 cut(s) 151, 599, 796
Cfr42I CCGCGG 1 cut(s) 388
CseI GACGC 2 cut(s) 214, 385
Csp6I GTAC 1 cut(s) 739
CviAII CATG 3 cut(s) 457, 470, 712
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 4 cut(s) 50, 701, 783, 800
DpnI GATC 6 cut(s) 200, 258, 266, 513, 555, 765
DpnII GATC 6 cut(s) 198, 256, 264, 511, 553, 763
DriI GACNNNNNGTC 1 cut(s) 91
Eam1104I CTCTTC 2 cut(s) 635, 677
Eam1105I GACNNNNNGTC 1 cut(s) 91
EarI CTCTTC 2 cut(s) 635, 677
EciI GGCGGA 1 cut(s) 164
Ecl136II GAGCTC 1 cut(s) 621
Eco24I GRGCYC 1 cut(s) 623
Eco47I GGWCC 2 cut(s) 599, 796
Eco47III AGCGCT 1 cut(s) 446
Eco53kI GAGCTC 1 cut(s) 621
Eco81I CCTNAGG 1 cut(s) 800
Eco88I CYCGRG 2 cut(s) 353, 481
EcoICRI GAGCTC 1 cut(s) 621
EcoO109I RGGNCCY 1 cut(s) 796
EcoRI GAATTC 1 cut(s) 664
EcoRII CCWGG 2 cut(s) 153, 236
EcoT38I GRGCYC 1 cut(s) 623
FaeI CATG 3 cut(s) 460, 473, 715
FaiI YATR 6 cut(s) 125, 440, 458, 471, 543, 713
FatI CATG 3 cut(s) 456, 469, 711
FauI CCCGC 2 cut(s) 328, 392
Fnu4HI GCNGC 6 cut(s) 13, 163, 223, 232, 388, 774
FokI GGATG 1 cut(s) 82
FriOI GRGCYC 1 cut(s) 623
Fsp4HI GCNGC 6 cut(s) 13, 163, 223, 232, 388, 774
FspBI CTAG 3 cut(s) 83, 402, 674
GlaI GCGC 2 cut(s) 235, 446
GluI GCNGC 6 cut(s) 13, 163, 223, 232, 388, 774
GsuI CTGGAG 2 cut(s) 202, 358
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 3 cut(s) 152, 158, 821
HapII CCGG 1 cut(s) 327
HgaI GACGC 2 cut(s) 214, 385
HhaI GCGC 2 cut(s) 236, 447
Hin1I GRCGYC 1 cut(s) 306
Hin1II CATG 3 cut(s) 460, 473, 715
Hin6I GCGC 2 cut(s) 234, 445
HinP1I GCGC 2 cut(s) 234, 445
HindIII AAGCTT 2 cut(s) 191, 331
HinfI GANTC 5 cut(s) 89, 474, 499, 695, 811
HpaII CCGG 1 cut(s) 327
HphI GGTGA 1 cut(s) 442
Hpy188I TCNGA 8 cut(s) 109, 198, 256, 314, 504, 670, 702, 768
Hpy188III TCNNGA 8 cut(s) 187, 260, 327, 337, 355, 515, 602, 692
Hpy99I CGWCG 9 cut(s) 20, 32, 35, 113, 251, 272, 311, 314, 401
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 2 cut(s) 306, 717
HpyCH4V TGCA 2 cut(s) 715, 776
HpyF10VI GCNNNNNNNGC 7 cut(s) 222, 228, 231, 295, 393, 402, 407
HpyF3I CTNAG 4 cut(s) 50, 701, 783, 800
HpySE526I ACGT 2 cut(s) 306, 717
Hsp92I GRCGYC 1 cut(s) 306
Hsp92II CATG 3 cut(s) 460, 473, 715
HspAI GCGC 2 cut(s) 234, 445
Kpn2I TCCGGA 1 cut(s) 326
KspI CCGCGG 1 cut(s) 388
Kzo9I GATC 6 cut(s) 198, 256, 264, 511, 553, 763
LmnI GCTCC 2 cut(s) 221, 530
Lsp1109I GCAGC 2 cut(s) 209, 760
LweI GCATC 1 cut(s) 715
MaeI CTAG 3 cut(s) 83, 402, 674
MaeII ACGT 2 cut(s) 306, 717
MalI GATC 6 cut(s) 200, 258, 266, 513, 555, 765
MboI GATC 6 cut(s) 198, 256, 264, 511, 553, 763
MboII GAAGA 5 cut(s) 32, 175, 605, 652, 694
MflI RGATCY 2 cut(s) 553, 763
MhlI GDGCHC 1 cut(s) 623
MluCI AATT 3 cut(s) 359, 378, 664
MlyI GAGTC 3 cut(s) 83, 704, 805
MmeI TCCRAC 2 cut(s) 132, 292
MroI TCCGGA 1 cut(s) 326
MseI TTAA 1 cut(s) 829
MslI CAYNNNNRTG 1 cut(s) 720
MspA1I CMGCKG 2 cut(s) 222, 387
MspI CCGG 1 cut(s) 327
MspR9I CCNGG 2 cut(s) 155, 238
MvaI CCWGG 2 cut(s) 155, 238
MvnI CGCG 3 cut(s) 165, 234, 387
MwoI GCNNNNNNNGC 7 cut(s) 222, 228, 231, 295, 393, 402, 407
NdeII GATC 6 cut(s) 198, 256, 264, 511, 553, 763
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 3 cut(s) 460, 473, 715
NlaIV GGNNCC 2 cut(s) 555, 765
NmeAIII GCCGAG 1 cut(s) 128
NspI RCATGY 1 cut(s) 715
PaeI GCATGC 1 cut(s) 715
PaeR7I CTCGAG 1 cut(s) 481
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 2 cut(s) 474, 499
PflFI GACNNNGTC 1 cut(s) 308
PkrI GCNGC 6 cut(s) 14, 164, 224, 233, 389, 775
PleI GAGTC 3 cut(s) 83, 703, 805
PpsI GAGTC 3 cut(s) 83, 703, 805
PpuMI RGGWCCY 1 cut(s) 796
Psp124BI GAGCTC 1 cut(s) 623
Psp5II RGGWCCY 1 cut(s) 796
Psp6I CCWGG 2 cut(s) 153, 236
PspGI CCWGG 2 cut(s) 153, 236
PspN4I GGNNCC 2 cut(s) 555, 765
PspPI GGNCC 3 cut(s) 151, 599, 796
PspPPI RGGWCCY 1 cut(s) 796
PspXI VCTCGAGB 1 cut(s) 481
PsuI RGATCY 2 cut(s) 553, 763
PsyI GACNNNGTC 1 cut(s) 308
PvuII CAGCTG 1 cut(s) 222
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
RseI CAYNNNNRTG 1 cut(s) 720
SacI GAGCTC 1 cut(s) 623
SacII CCGCGG 1 cut(s) 388
SaqAI TTAA 1 cut(s) 829
SatI GCNGC 6 cut(s) 13, 163, 223, 232, 388, 774
Sau3AI GATC 6 cut(s) 198, 256, 264, 511, 553, 763
Sau96I GGNCC 3 cut(s) 151, 599, 796
SchI GAGTC 3 cut(s) 83, 704, 805
ScrFI CCNGG 2 cut(s) 155, 238
SduI GDGCHC 1 cut(s) 623
SfaNI GCATC 1 cut(s) 715
Sfr274I CTCGAG 1 cut(s) 481
Sfr303I CCGCGG 1 cut(s) 388
SgrBI CCGCGG 1 cut(s) 388
SinI GGWCC 2 cut(s) 599, 796
SlaI CTCGAG 1 cut(s) 481
SmiMI CAYNNNNRTG 1 cut(s) 720
SmlI CTYRAG 3 cut(s) 299, 481, 692
SmoI CTYRAG 3 cut(s) 299, 481, 692
SphI GCATGC 1 cut(s) 715
Sse9I AATT 3 cut(s) 359, 378, 664
SsiI CCGC 8 cut(s) 12, 163, 175, 232, 321, 385, 387, 578
SspMI CTAG 3 cut(s) 83, 402, 674
SstI GAGCTC 1 cut(s) 623
StyD4I CCNGG 2 cut(s) 153, 236
TaiI ACGT 2 cut(s) 309, 720
TaqI TCGA 3 cut(s) 186, 482, 546
TasI AATT 3 cut(s) 359, 378, 664
TatI WGTACW 1 cut(s) 738
TauI GCSGC 4 cut(s) 15, 165, 234, 390
TfiI GAWTC 2 cut(s) 474, 499
Tru1I TTAA 1 cut(s) 829
Tru9I TTAA 1 cut(s) 829
TscAI CASTG 2 cut(s) 661, 736
TseI GCWGC 2 cut(s) 222, 773
TspDTI ATGAA 1 cut(s) 149
TspGWI ACGGA 2 cut(s) 235, 412
TspRI CASTG 2 cut(s) 661, 736
Tth111I GACNNNGTC 1 cut(s) 308
VpaK11BI GGWCC 2 cut(s) 599, 796
XapI RAATTY 2 cut(s) 359, 664
XceI RCATGY 1 cut(s) 715
XhoI CTCGAG 1 cut(s) 481
XspI CTAG 3 cut(s) 83, 402, 674
ZraI GACGTC 1 cut(s) 307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.