Rw3G004970

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013410
Physical Location & Seq
Forward (+)
4173076 .. 4174498
1423 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G004970.1

Sequence Viewer

Length: 846 bp
ATGGGTTGTTGGGGCGACGAAGAAAACGACGACGAAATCCTCAACCCTCTCAGCCCCACCTCCACCACCACCACCACCACCACCACCACCCAAACCCTAGACGACTCGTCATCCTCCTCCTCCGACGGCTACACCACCATATCCCCGATGAACTCTCACTTCTCGGCCCTGGCCTGCCGCGACACCCTCCGCCTCATCTTCGAGCAGCTTCCGATCCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGCGCCTGGAACTCCGTCGCCTCCGATCAAGAGATCGTCGCCAACGCCTTCAAATCGCCGTGGAAGCTCAAGGACGTCGTCGGAAAACCCGCCTCCGGAAGCTTCTGGAGAGATAACAGCCTCGGGAAATTCGCCATCTCACACCGAATTGCCCGCGGCGATAGCGTCGCTAGCCTCGCCGTCAGGTATTCCGTTCAGGTGATGGACATAAAGCGCTTGAACAACATGATGAGCGAGCATGGAATCTACTCGAGGGAGAGGTTGTTGATTCCGATAAGCGATCCTGAAATGCTTGTGGGAGCTACCTGCTATGTCGAAGTGGATCCCTGTGCCAAGAGAGAAGTTGCGGTGTTGTATTTGGAAGATGGTCCTGACCAGAAATCAATGAGCTCTTGCTCTTTGTCGCAGAAGAGTGCCGACAGCAGTGGCTTGAATTCTGAACTAGGTAAGAAGAGGGTTCTTGAGTCTCTGAGGAGAAGCATGCACGTTGATGATGCCACTGCTCTGTACTACTTGTCCATTGCTGATGGGGATCCGAGAGCTGCATTGTCTCAGTTCTCGCAGGACCTTAGGTGGGAGACTCATTCGGCCTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

281

Amino Acids

30.87

Weight (kDa)

5.09

Isoelectric Point (pI)

46.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 60 - 94 2.1e-07 F-box domain
F-box-like PF12937 63 - 93 1.4e-07 F-box-like
LysM PF01476 130 - 173 2.8e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 324
Acc36I ACCTGC 1 cut(s) 560
AccII CGCG 3 cut(s) 180, 249, 402
AccIII TCCGGA 1 cut(s) 341
AciI CCGC 7 cut(s) 178, 190, 247, 336, 400, 402, 593
AclWI GGATC 6 cut(s) 208, 521, 563, 576, 773, 786
AcsI RAATTY 2 cut(s) 374, 679
AcyI GRCGYC 1 cut(s) 321
AfaI GTAC 1 cut(s) 755
AfeI AGCGCT 1 cut(s) 461
AfiI CCNNNNNNNGG 2 cut(s) 341, 820
AgsI TTSAA 3 cut(s) 298, 466, 679
AhdI GACNNNNNGTC 1 cut(s) 106
AjnI CCWGG 2 cut(s) 168, 251
AluBI AGCT 7 cut(s) 208, 237, 313, 348, 548, 636, 788
AluI AGCT 7 cut(s) 208, 237, 313, 348, 548, 636, 788
Alw21I GWGCWC 1 cut(s) 638
Alw26I GTCTC 3 cut(s) 717, 801, 818
AlwI GGATC 6 cut(s) 208, 521, 563, 576, 773, 786
Ama87I CYCGRG 2 cut(s) 368, 496
Aor13HI TCCGGA 1 cut(s) 341
Aor51HI AGCGCT 1 cut(s) 461
AoxI GGCC 3 cut(s) 165, 171, 834
ApeKI GCWGC 3 cut(s) 205, 237, 788
ApoI RAATTY 2 cut(s) 374, 679
AspLEI GCGC 2 cut(s) 251, 462
AspS9I GGNCC 3 cut(s) 166, 614, 811
AsuHPI GGTGA 1 cut(s) 457
AsuNHI GCTAGC 1 cut(s) 416
AvaI CYCGRG 2 cut(s) 368, 496
AvaII GGWCC 2 cut(s) 614, 811
AxyI CCTNAGG 1 cut(s) 815
BamHI GGATCC 2 cut(s) 568, 778
BanII GRGCYC 1 cut(s) 638
Bbv12I GWGCWC 1 cut(s) 638
BbvI GCAGC 3 cut(s) 217, 224, 775
BccI CCATC 4 cut(s) 389, 442, 605, 767
BceAI ACGGC 3 cut(s) 142, 289, 410
BciT130I CCWGG 2 cut(s) 170, 253
BcoDI GTCTC 3 cut(s) 717, 801, 818
BfaI CTAG 3 cut(s) 98, 417, 689
BfoI RGCGCY 1 cut(s) 463
BfuAI ACCTGC 1 cut(s) 560
BisI GCNGC 6 cut(s) 178, 206, 238, 247, 403, 789
BlsI GCNGC 6 cut(s) 179, 207, 239, 248, 404, 790
Bme1390I CCNGG 2 cut(s) 170, 253
Bme18I GGWCC 2 cut(s) 614, 811
BmeRI GACNNNNNGTC 1 cut(s) 106
BmeT110I CYCGRG 2 cut(s) 368, 496
BmgT120I GGNCC 3 cut(s) 166, 614, 811
BmiI GGNNCC 2 cut(s) 570, 780
BmrFI CCNGG 2 cut(s) 170, 253
BmsI GCATC 1 cut(s) 730
BmtI GCTAGC 1 cut(s) 420
BpmI CTGGAG 2 cut(s) 217, 373
BpuEI CTTGAG 2 cut(s) 299, 728
BsaBI GATNNNNATC 1 cut(s) 777
BsaHI GRCGYC 1 cut(s) 321
BsaJI CCNNGG 4 cut(s) 168, 305, 367, 400
BsaWI WCCGGW 1 cut(s) 341
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 2 cut(s) 341, 820
Bse21I CCTNAGG 1 cut(s) 815
Bse3DI GCAATG 1 cut(s) 765
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 341
BseBI CCWGG 2 cut(s) 170, 253
BseDI CCNNGG 4 cut(s) 168, 305, 367, 400
BseGI GGATG 1 cut(s) 110
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 2 cut(s) 341, 820
BseMI GCAATG 1 cut(s) 765
BseMII CTCAG 3 cut(s) 64, 707, 812
BseRI GAGGAG 3 cut(s) 106, 109, 733
BseXI GCAGC 3 cut(s) 217, 224, 775
Bsh1236I CGCG 3 cut(s) 180, 249, 402
BshFI GGCC 3 cut(s) 167, 173, 836
BsiHKAI GWGCWC 1 cut(s) 638
BsiHKCI CYCGRG 2 cut(s) 368, 496
BsiSI CCGG 1 cut(s) 342
BslI CCNNNNNNNGG 2 cut(s) 341, 820
BsmAI GTCTC 3 cut(s) 717, 801, 818
BsnI GGCC 3 cut(s) 167, 173, 836
BsoBI CYCGRG 2 cut(s) 368, 496
Bsp1286I GDGCHC 1 cut(s) 638
Bsp13I TCCGGA 1 cut(s) 341
Bsp143I GATC 6 cut(s) 213, 271, 279, 526, 568, 778
BspACI CCGC 7 cut(s) 178, 190, 247, 336, 400, 402, 593
BspANI GGCC 3 cut(s) 167, 173, 836
BspCNI CTCAG 3 cut(s) 63, 708, 811
BspEI TCCGGA 1 cut(s) 341
BspFNI CGCG 3 cut(s) 180, 249, 402
BspLI GGNNCC 2 cut(s) 570, 780
BspMI ACCTGC 1 cut(s) 560
BspOI GCTAGC 1 cut(s) 420
BspPI GGATC 6 cut(s) 208, 521, 563, 576, 773, 786
BsrDI GCAATG 1 cut(s) 765
BssECI CCNNGG 4 cut(s) 168, 305, 367, 400
BssMI GATC 6 cut(s) 213, 271, 279, 526, 568, 778
BssNI GRCGYC 1 cut(s) 321
Bst2UI CCWGG 2 cut(s) 170, 253
Bst6I CTCTTC 2 cut(s) 650, 692
BstACI GRCGYC 1 cut(s) 321
BstC8I GCNNGC 6 cut(s) 175, 229, 400, 418, 482, 728
BstDEI CTNAG 4 cut(s) 50, 716, 798, 815
BstDSI CCRYGG 2 cut(s) 305, 400
BstF5I GGATG 1 cut(s) 110
BstFNI CGCG 3 cut(s) 180, 249, 402
BstH2I RGCGCY 1 cut(s) 463
BstHHI GCGC 2 cut(s) 251, 462
BstKTI GATC 6 cut(s) 216, 274, 282, 529, 571, 781
BstMAI GTCTC 3 cut(s) 717, 801, 818
BstMBI GATC 6 cut(s) 213, 271, 279, 526, 568, 778
BstMWI GCNNNNNNNGC 7 cut(s) 237, 243, 246, 310, 408, 417, 422
BstNI CCWGG 2 cut(s) 170, 253
BstNSI RCATGY 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 168, 251
BstUI CGCG 3 cut(s) 180, 249, 402
BstV1I GCAGC 3 cut(s) 217, 224, 775
BstX2I RGATCY 2 cut(s) 568, 778
BstYI RGATCY 2 cut(s) 568, 778
Bsu36I CCTNAGG 1 cut(s) 815
BsuRI GGCC 3 cut(s) 167, 173, 836
BtgI CCRYGG 2 cut(s) 305, 400
BtsCI GGATG 1 cut(s) 110
BtsI GCAGTG 2 cut(s) 676, 744
BtsIMutI CAGTG 2 cut(s) 676, 744
BveI ACCTGC 1 cut(s) 560
Cac8I GCNNGC 6 cut(s) 175, 229, 400, 418, 482, 728
CfoI GCGC 2 cut(s) 251, 462
Cfr13I GGNCC 3 cut(s) 166, 614, 811
Cfr42I CCGCGG 1 cut(s) 403
CseI GACGC 2 cut(s) 229, 400
Csp6I GTAC 1 cut(s) 754
CviAII CATG 3 cut(s) 472, 485, 727
CviQI GTAC 1 cut(s) 754
DdeI CTNAG 4 cut(s) 50, 716, 798, 815
DpnI GATC 6 cut(s) 215, 273, 281, 528, 570, 780
DpnII GATC 6 cut(s) 213, 271, 279, 526, 568, 778
DriI GACNNNNNGTC 1 cut(s) 106
Eam1104I CTCTTC 2 cut(s) 650, 692
Eam1105I GACNNNNNGTC 1 cut(s) 106
EarI CTCTTC 2 cut(s) 650, 692
EciI GGCGGA 1 cut(s) 179
Ecl136II GAGCTC 1 cut(s) 636
Eco24I GRGCYC 1 cut(s) 638
Eco47I GGWCC 2 cut(s) 614, 811
Eco47III AGCGCT 1 cut(s) 461
Eco53kI GAGCTC 1 cut(s) 636
Eco81I CCTNAGG 1 cut(s) 815
Eco88I CYCGRG 2 cut(s) 368, 496
EcoICRI GAGCTC 1 cut(s) 636
EcoO109I RGGNCCY 1 cut(s) 811
EcoRI GAATTC 1 cut(s) 679
EcoRII CCWGG 2 cut(s) 168, 251
EcoT38I GRGCYC 1 cut(s) 638
FaeI CATG 3 cut(s) 475, 488, 730
FaiI YATR 6 cut(s) 140, 455, 473, 486, 558, 728
FatI CATG 3 cut(s) 471, 484, 726
FauI CCCGC 2 cut(s) 343, 407
Fnu4HI GCNGC 6 cut(s) 178, 206, 238, 247, 403, 789
FokI GGATG 1 cut(s) 97
FriOI GRGCYC 1 cut(s) 638
Fsp4HI GCNGC 6 cut(s) 178, 206, 238, 247, 403, 789
FspBI CTAG 3 cut(s) 98, 417, 689
GlaI GCGC 2 cut(s) 250, 461
GluI GCNGC 6 cut(s) 178, 206, 238, 247, 403, 789
GsuI CTGGAG 2 cut(s) 217, 373
HaeII RGCGCY 1 cut(s) 463
HaeIII GGCC 3 cut(s) 167, 173, 836
HapII CCGG 1 cut(s) 342
HgaI GACGC 2 cut(s) 229, 400
HhaI GCGC 2 cut(s) 251, 462
Hin1I GRCGYC 1 cut(s) 321
Hin1II CATG 3 cut(s) 475, 488, 730
Hin6I GCGC 2 cut(s) 249, 460
HinP1I GCGC 2 cut(s) 249, 460
HindIII AAGCTT 1 cut(s) 346
HinfI GANTC 5 cut(s) 104, 489, 514, 710, 826
HpaII CCGG 1 cut(s) 342
HphI GGTGA 1 cut(s) 457
Hpy188I TCNGA 8 cut(s) 124, 213, 271, 329, 519, 685, 717, 783
Hpy188III TCNNGA 7 cut(s) 275, 342, 352, 370, 530, 617, 707
Hpy99I CGWCG 9 cut(s) 20, 32, 35, 128, 266, 287, 326, 329, 416
HpyAV CCTTC 1 cut(s) 304
HpyCH4IV ACGT 2 cut(s) 321, 732
HpyCH4V TGCA 2 cut(s) 730, 791
HpyF10VI GCNNNNNNNGC 7 cut(s) 237, 243, 246, 310, 408, 417, 422
HpyF3I CTNAG 4 cut(s) 50, 716, 798, 815
HpySE526I ACGT 2 cut(s) 321, 732
Hsp92I GRCGYC 1 cut(s) 321
Hsp92II CATG 3 cut(s) 475, 488, 730
HspAI GCGC 2 cut(s) 249, 460
Kpn2I TCCGGA 1 cut(s) 341
KspI CCGCGG 1 cut(s) 403
Kzo9I GATC 6 cut(s) 213, 271, 279, 526, 568, 778
LmnI GCTCC 2 cut(s) 236, 545
Lsp1109I GCAGC 3 cut(s) 217, 224, 775
LweI GCATC 1 cut(s) 730
MaeI CTAG 3 cut(s) 98, 417, 689
MaeII ACGT 2 cut(s) 321, 732
MalI GATC 6 cut(s) 215, 273, 281, 528, 570, 780
MboI GATC 6 cut(s) 213, 271, 279, 526, 568, 778
MboII GAAGA 5 cut(s) 32, 190, 620, 667, 709
MflI RGATCY 2 cut(s) 568, 778
MhlI GDGCHC 1 cut(s) 638
MluCI AATT 3 cut(s) 374, 393, 679
MlyI GAGTC 3 cut(s) 98, 719, 820
MmeI TCCRAC 2 cut(s) 147, 307
MroI TCCGGA 1 cut(s) 341
MseI TTAA 1 cut(s) 844
MslI CAYNNNNRTG 1 cut(s) 735
MspA1I CMGCKG 2 cut(s) 237, 402
MspI CCGG 1 cut(s) 342
MspR9I CCNGG 2 cut(s) 170, 253
MvaI CCWGG 2 cut(s) 170, 253
MvnI CGCG 3 cut(s) 180, 249, 402
MwoI GCNNNNNNNGC 7 cut(s) 237, 243, 246, 310, 408, 417, 422
NdeII GATC 6 cut(s) 213, 271, 279, 526, 568, 778
NheI GCTAGC 1 cut(s) 416
NlaIII CATG 3 cut(s) 475, 488, 730
NlaIV GGNNCC 2 cut(s) 570, 780
NmeAIII GCCGAG 1 cut(s) 143
NspI RCATGY 1 cut(s) 730
PaeI GCATGC 1 cut(s) 730
PaeR7I CTCGAG 1 cut(s) 496
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 2 cut(s) 489, 514
PflFI GACNNNGTC 1 cut(s) 323
PkrI GCNGC 6 cut(s) 179, 207, 239, 248, 404, 790
PleI GAGTC 3 cut(s) 98, 718, 820
PpsI GAGTC 3 cut(s) 98, 718, 820
PpuMI RGGWCCY 1 cut(s) 811
Psp124BI GAGCTC 1 cut(s) 638
Psp5II RGGWCCY 1 cut(s) 811
Psp6I CCWGG 2 cut(s) 168, 251
PspGI CCWGG 2 cut(s) 168, 251
PspN4I GGNNCC 2 cut(s) 570, 780
PspPI GGNCC 3 cut(s) 166, 614, 811
PspPPI RGGWCCY 1 cut(s) 811
PspXI VCTCGAGB 1 cut(s) 496
PsuI RGATCY 2 cut(s) 568, 778
PsyI GACNNNGTC 1 cut(s) 323
PvuII CAGCTG 1 cut(s) 237
RsaI GTAC 1 cut(s) 755
RsaNI GTAC 1 cut(s) 754
RseI CAYNNNNRTG 1 cut(s) 735
SacI GAGCTC 1 cut(s) 638
SacII CCGCGG 1 cut(s) 403
SaqAI TTAA 1 cut(s) 844
SatI GCNGC 6 cut(s) 178, 206, 238, 247, 403, 789
Sau3AI GATC 6 cut(s) 213, 271, 279, 526, 568, 778
Sau96I GGNCC 3 cut(s) 166, 614, 811
SchI GAGTC 3 cut(s) 98, 719, 820
ScrFI CCNGG 2 cut(s) 170, 253
SduI GDGCHC 1 cut(s) 638
SfaNI GCATC 1 cut(s) 730
Sfr274I CTCGAG 1 cut(s) 496
Sfr303I CCGCGG 1 cut(s) 403
SgrBI CCGCGG 1 cut(s) 403
SinI GGWCC 2 cut(s) 614, 811
SlaI CTCGAG 1 cut(s) 496
SmiMI CAYNNNNRTG 1 cut(s) 735
SmlI CTYRAG 3 cut(s) 314, 496, 707
SmoI CTYRAG 3 cut(s) 314, 496, 707
SphI GCATGC 1 cut(s) 730
Sse9I AATT 3 cut(s) 374, 393, 679
SsiI CCGC 7 cut(s) 178, 190, 247, 336, 400, 402, 593
SspMI CTAG 3 cut(s) 98, 417, 689
SstI GAGCTC 1 cut(s) 638
StyD4I CCNGG 2 cut(s) 168, 251
TaiI ACGT 2 cut(s) 324, 735
TaqI TCGA 3 cut(s) 201, 497, 561
TasI AATT 3 cut(s) 374, 393, 679
TatI WGTACW 1 cut(s) 753
TauI GCSGC 3 cut(s) 180, 249, 405
TfiI GAWTC 2 cut(s) 489, 514
Tru1I TTAA 1 cut(s) 844
Tru9I TTAA 1 cut(s) 844
TscAI CASTG 2 cut(s) 676, 751
TseI GCWGC 3 cut(s) 205, 237, 788
TspDTI ATGAA 1 cut(s) 164
TspGWI ACGGA 2 cut(s) 250, 427
TspRI CASTG 2 cut(s) 676, 751
Tth111I GACNNNGTC 1 cut(s) 323
VpaK11BI GGWCC 2 cut(s) 614, 811
XapI RAATTY 2 cut(s) 374, 679
XceI RCATGY 1 cut(s) 730
XhoI CTCGAG 1 cut(s) 496
XspI CTAG 3 cut(s) 98, 417, 689
ZraI GACGTC 1 cut(s) 322
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.