RLG00000025396

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013410
Physical Location & Seq
Reverse (-)
45543892 .. 45544963
1072 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025396

Sequence Viewer

Length: 834 bp
ATGGGTTGCTGCGGCGACGAAGAAAACGACGACGATATTCTCAACCCTCTCAGCCCCACCTCCTCCACCACCACCACCCAAACCCCAGACGACTCGTCGTCCTCCTCCTCCGACAGCCACACCACCATATCCCCGATGAACTCTCACTTCTCGGCCCTGGCGTGCCGCGACACCCTCCGCCTCATCTTCGAGAAGCTTCCGATCCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGCGCCTGGAACTCCGTCGCCTCCGATCAAGAGATCGTCGCCAACTCCTTCAAATCGCCGTGGAAGCTCAAGGACGTCGTCGGAAAACCCGCCTCCGGAAGCTTCTGGAGAGATAACAGCCTCGGCAAATTCGCCATCTCACACCGAATTTCCCGCGGCGATAGCGTCGCTAGCCTCGCCGTCAAGTATTCCGTTCAGGTGATGGACATAAAGCGCTTGAACAACATGATGAGCGAGCATGGAATCTACTCGAGGGAGAGGTTGTTGATTCCGATAAGCGATCCTGAAATGCTTGTGGGAGCTACCTGCTATGTTGAAGTGGATCCCTGTGCTAAGAGAGAAGTAGCGGTGTTGTATTTGGATGATGGTCCTGACCAGAAATCAATGAGCTCTTGCTCTTTGTCGCAGACAAGTGCCGACAGCAGTGGCTGGAATTCTGAACTAGGTAAGAAGAGGGTTCTCGAGTCTCTGAGGAGAAGCATGCAGGTTGATGATGCCACTGCTCTGTACTACTTGTCCATTGCTGATGGGGATCCGAGAGCTGCATTGTCTCAGTTCTCGCAGGACCTTAGGTGGGAGACTCATTCGGCCTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

278

Amino Acids

30.37

Weight (kDa)

5.07

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 56 - 90 1.8e-07 F-box domain
F-box-like PF12937 59 - 89 1.9e-07 F-box-like
LysM PF01476 126 - 169 5.1e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 312
Acc36I ACCTGC 2 cut(s) 548, 709
AccII CGCG 3 cut(s) 168, 237, 390
AccIII TCCGGA 1 cut(s) 329
AciI CCGC 8 cut(s) 12, 166, 178, 235, 324, 388, 390, 581
AclWI GGATC 6 cut(s) 196, 509, 551, 564, 761, 774
AcsI RAATTY 3 cut(s) 362, 381, 667
AcyI GRCGYC 1 cut(s) 309
AfaI GTAC 1 cut(s) 743
AfeI AGCGCT 1 cut(s) 449
AfiI CCNNNNNNNGG 2 cut(s) 329, 808
AgsI TTSAA 3 cut(s) 286, 454, 551
AhdI GACNNNNNGTC 2 cut(s) 94, 97
AjnI CCWGG 2 cut(s) 156, 239
AluBI AGCT 7 cut(s) 196, 225, 301, 336, 536, 624, 776
AluI AGCT 7 cut(s) 196, 225, 301, 336, 536, 624, 776
Alw21I GWGCWC 1 cut(s) 626
Alw26I GTCTC 3 cut(s) 705, 789, 806
AlwI GGATC 6 cut(s) 196, 509, 551, 564, 761, 774
AlwNI CAGNNNCTG 1 cut(s) 663
Ama87I CYCGRG 2 cut(s) 484, 695
Aor13HI TCCGGA 1 cut(s) 329
Aor51HI AGCGCT 1 cut(s) 449
AoxI GGCC 2 cut(s) 153, 822
ApeKI GCWGC 3 cut(s) 9, 225, 776
ApoI RAATTY 3 cut(s) 362, 381, 667
AspLEI GCGC 2 cut(s) 239, 450
AspS9I GGNCC 3 cut(s) 154, 602, 799
AsuHPI GGTGA 1 cut(s) 445
AsuNHI GCTAGC 1 cut(s) 404
AvaI CYCGRG 2 cut(s) 484, 695
AvaII GGWCC 2 cut(s) 602, 799
AxyI CCTNAGG 1 cut(s) 803
BamHI GGATCC 2 cut(s) 556, 766
BanII GRGCYC 1 cut(s) 626
Bbv12I GWGCWC 1 cut(s) 626
BbvI GCAGC 2 cut(s) 212, 763
BccI CCATC 4 cut(s) 377, 430, 593, 755
BceAI ACGGC 2 cut(s) 277, 398
BciT130I CCWGG 2 cut(s) 158, 241
BcoDI GTCTC 3 cut(s) 705, 789, 806
BfaI CTAG 2 cut(s) 405, 677
BfoI RGCGCY 1 cut(s) 451
BfuAI ACCTGC 2 cut(s) 548, 709
BisI GCNGC 7 cut(s) 10, 13, 166, 226, 235, 391, 777
BlsI GCNGC 7 cut(s) 11, 14, 167, 227, 236, 392, 778
Bme1390I CCNGG 2 cut(s) 158, 241
Bme18I GGWCC 2 cut(s) 602, 799
BmeRI GACNNNNNGTC 2 cut(s) 94, 97
BmeT110I CYCGRG 2 cut(s) 484, 695
BmgT120I GGNCC 3 cut(s) 154, 602, 799
BmiI GGNNCC 2 cut(s) 558, 768
BmrFI CCNGG 2 cut(s) 158, 241
BmsI GCATC 1 cut(s) 718
BmtI GCTAGC 1 cut(s) 408
BpmI CTGGAG 2 cut(s) 205, 361
BpuEI CTTGAG 1 cut(s) 287
BsaBI GATNNNNATC 1 cut(s) 765
BsaHI GRCGYC 1 cut(s) 309
BsaJI CCNNGG 4 cut(s) 156, 293, 355, 388
BsaWI WCCGGW 1 cut(s) 329
BsaXI ACNNNNNCTCC 2 cut(s) 482, 512
Bsc4I CCNNNNNNNGG 2 cut(s) 329, 808
Bse21I CCTNAGG 1 cut(s) 803
Bse3DI GCAATG 1 cut(s) 753
Bse8I GATNNNNATC 1 cut(s) 765
BseAI TCCGGA 1 cut(s) 329
BseBI CCWGG 2 cut(s) 158, 241
BseDI CCNNGG 4 cut(s) 156, 293, 355, 388
BseGI GGATG 1 cut(s) 601
BseJI GATNNNNATC 1 cut(s) 765
BseLI CCNNNNNNNGG 2 cut(s) 329, 808
BseMI GCAATG 1 cut(s) 753
BseMII CTCAG 3 cut(s) 64, 695, 800
BseRI GAGGAG 4 cut(s) 52, 94, 97, 721
BseXI GCAGC 2 cut(s) 212, 763
Bsh1236I CGCG 3 cut(s) 168, 237, 390
BshFI GGCC 2 cut(s) 155, 824
BsiHKAI GWGCWC 1 cut(s) 626
BsiHKCI CYCGRG 2 cut(s) 484, 695
BsiSI CCGG 1 cut(s) 330
BslI CCNNNNNNNGG 2 cut(s) 329, 808
BsmAI GTCTC 3 cut(s) 705, 789, 806
BsnI GGCC 2 cut(s) 155, 824
BsoBI CYCGRG 2 cut(s) 484, 695
Bsp1286I GDGCHC 1 cut(s) 626
Bsp13I TCCGGA 1 cut(s) 329
Bsp143I GATC 6 cut(s) 201, 259, 267, 514, 556, 766
BspACI CCGC 8 cut(s) 12, 166, 178, 235, 324, 388, 390, 581
BspANI GGCC 2 cut(s) 155, 824
BspCNI CTCAG 3 cut(s) 63, 696, 799
BspEI TCCGGA 1 cut(s) 329
BspFNI CGCG 3 cut(s) 168, 237, 390
BspLI GGNNCC 2 cut(s) 558, 768
BspMI ACCTGC 2 cut(s) 548, 709
BspOI GCTAGC 1 cut(s) 408
BspPI GGATC 6 cut(s) 196, 509, 551, 564, 761, 774
BsrDI GCAATG 1 cut(s) 753
BssECI CCNNGG 4 cut(s) 156, 293, 355, 388
BssMI GATC 6 cut(s) 201, 259, 267, 514, 556, 766
BssNI GRCGYC 1 cut(s) 309
Bst2UI CCWGG 2 cut(s) 158, 241
Bst6I CTCTTC 1 cut(s) 680
BstACI GRCGYC 1 cut(s) 309
BstC8I GCNNGC 5 cut(s) 163, 217, 406, 470, 716
BstDEI CTNAG 5 cut(s) 50, 567, 704, 786, 803
BstDSI CCRYGG 2 cut(s) 293, 388
BstF5I GGATG 1 cut(s) 601
BstFNI CGCG 3 cut(s) 168, 237, 390
BstH2I RGCGCY 1 cut(s) 451
BstHHI GCGC 2 cut(s) 239, 450
BstKTI GATC 6 cut(s) 204, 262, 270, 517, 559, 769
BstMAI GTCTC 3 cut(s) 705, 789, 806
BstMBI GATC 6 cut(s) 201, 259, 267, 514, 556, 766
BstMWI GCNNNNNNNGC 7 cut(s) 225, 231, 234, 298, 396, 405, 410
BstNI CCWGG 2 cut(s) 158, 241
BstNSI RCATGY 1 cut(s) 718
BstSCI CCNGG 2 cut(s) 156, 239
BstUI CGCG 3 cut(s) 168, 237, 390
BstV1I GCAGC 2 cut(s) 212, 763
BstX2I RGATCY 2 cut(s) 556, 766
BstYI RGATCY 2 cut(s) 556, 766
Bsu36I CCTNAGG 1 cut(s) 803
BsuRI GGCC 2 cut(s) 155, 824
BtgI CCRYGG 2 cut(s) 293, 388
BtsCI GGATG 1 cut(s) 601
BtsI GCAGTG 2 cut(s) 664, 732
BtsIMutI CAGTG 2 cut(s) 664, 732
BveI ACCTGC 2 cut(s) 548, 709
Cac8I GCNNGC 5 cut(s) 163, 217, 406, 470, 716
CaiI CAGNNNCTG 1 cut(s) 663
CfoI GCGC 2 cut(s) 239, 450
Cfr13I GGNCC 3 cut(s) 154, 602, 799
Cfr42I CCGCGG 1 cut(s) 391
CseI GACGC 2 cut(s) 217, 388
Csp6I GTAC 1 cut(s) 742
CviAII CATG 3 cut(s) 460, 473, 715
CviQI GTAC 1 cut(s) 742
DdeI CTNAG 5 cut(s) 50, 567, 704, 786, 803
DpnI GATC 6 cut(s) 203, 261, 269, 516, 558, 768
DpnII GATC 6 cut(s) 201, 259, 267, 514, 556, 766
DriI GACNNNNNGTC 2 cut(s) 94, 97
Eam1104I CTCTTC 1 cut(s) 680
Eam1105I GACNNNNNGTC 2 cut(s) 94, 97
EarI CTCTTC 1 cut(s) 680
EciI GGCGGA 1 cut(s) 167
Ecl136II GAGCTC 1 cut(s) 624
Eco24I GRGCYC 1 cut(s) 626
Eco47I GGWCC 2 cut(s) 602, 799
Eco47III AGCGCT 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 624
Eco81I CCTNAGG 1 cut(s) 803
Eco88I CYCGRG 2 cut(s) 484, 695
EcoICRI GAGCTC 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 799
EcoRI GAATTC 1 cut(s) 667
EcoRII CCWGG 2 cut(s) 156, 239
EcoT38I GRGCYC 1 cut(s) 626
FaeI CATG 3 cut(s) 463, 476, 718
FaiI YATR 6 cut(s) 128, 443, 461, 474, 546, 716
FatI CATG 3 cut(s) 459, 472, 714
FauI CCCGC 2 cut(s) 331, 395
Fnu4HI GCNGC 7 cut(s) 10, 13, 166, 226, 235, 391, 777
FokI GGATG 1 cut(s) 608
FriOI GRGCYC 1 cut(s) 626
Fsp4HI GCNGC 7 cut(s) 10, 13, 166, 226, 235, 391, 777
FspBI CTAG 2 cut(s) 405, 677
GlaI GCGC 2 cut(s) 238, 449
GluI GCNGC 7 cut(s) 10, 13, 166, 226, 235, 391, 777
GsuI CTGGAG 2 cut(s) 205, 361
HaeII RGCGCY 1 cut(s) 451
HaeIII GGCC 2 cut(s) 155, 824
HapII CCGG 1 cut(s) 330
HgaI GACGC 2 cut(s) 217, 388
HhaI GCGC 2 cut(s) 239, 450
Hin1I GRCGYC 1 cut(s) 309
Hin1II CATG 3 cut(s) 463, 476, 718
Hin6I GCGC 2 cut(s) 237, 448
HinP1I GCGC 2 cut(s) 237, 448
HindIII AAGCTT 2 cut(s) 194, 334
HinfI GANTC 5 cut(s) 92, 477, 502, 698, 814
HpaII CCGG 1 cut(s) 330
HphI GGTGA 1 cut(s) 445
Hpy188I TCNGA 8 cut(s) 112, 201, 259, 317, 507, 673, 705, 771
Hpy188III TCNNGA 7 cut(s) 190, 263, 330, 340, 518, 605, 695
Hpy99I CGWCG 9 cut(s) 20, 32, 35, 100, 254, 275, 314, 317, 404
HpyAV CCTTC 1 cut(s) 292
HpyCH4IV ACGT 1 cut(s) 309
HpyCH4V TGCA 2 cut(s) 718, 779
HpyF10VI GCNNNNNNNGC 7 cut(s) 225, 231, 234, 298, 396, 405, 410
HpyF3I CTNAG 5 cut(s) 50, 567, 704, 786, 803
HpySE526I ACGT 1 cut(s) 309
Hsp92I GRCGYC 1 cut(s) 309
Hsp92II CATG 3 cut(s) 463, 476, 718
HspAI GCGC 2 cut(s) 237, 448
Kpn2I TCCGGA 1 cut(s) 329
KspI CCGCGG 1 cut(s) 391
Kzo9I GATC 6 cut(s) 201, 259, 267, 514, 556, 766
LmnI GCTCC 2 cut(s) 224, 533
Lsp1109I GCAGC 2 cut(s) 212, 763
LweI GCATC 1 cut(s) 718
MaeI CTAG 2 cut(s) 405, 677
MaeII ACGT 1 cut(s) 309
MalI GATC 6 cut(s) 203, 261, 269, 516, 558, 768
MboI GATC 6 cut(s) 201, 259, 267, 514, 556, 766
MboII GAAGA 3 cut(s) 32, 178, 697
MflI RGATCY 2 cut(s) 556, 766
MhlI GDGCHC 1 cut(s) 626
MluCI AATT 3 cut(s) 362, 381, 667
MlyI GAGTC 3 cut(s) 86, 707, 808
MmeI TCCRAC 2 cut(s) 135, 295
MroI TCCGGA 1 cut(s) 329
MseI TTAA 1 cut(s) 832
MspA1I CMGCKG 2 cut(s) 225, 390
MspI CCGG 1 cut(s) 330
MspR9I CCNGG 2 cut(s) 158, 241
MvaI CCWGG 2 cut(s) 158, 241
MvnI CGCG 3 cut(s) 168, 237, 390
MwoI GCNNNNNNNGC 7 cut(s) 225, 231, 234, 298, 396, 405, 410
NdeII GATC 6 cut(s) 201, 259, 267, 514, 556, 766
NheI GCTAGC 1 cut(s) 404
NlaIII CATG 3 cut(s) 463, 476, 718
NlaIV GGNNCC 2 cut(s) 558, 768
NmeAIII GCCGAG 2 cut(s) 131, 336
NspI RCATGY 1 cut(s) 718
PaeI GCATGC 1 cut(s) 718
PaeR7I CTCGAG 2 cut(s) 484, 695
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 158
PfeI GAWTC 2 cut(s) 477, 502
PflFI GACNNNGTC 1 cut(s) 311
PkrI GCNGC 7 cut(s) 11, 14, 167, 227, 236, 392, 778
PleI GAGTC 3 cut(s) 86, 706, 808
PpsI GAGTC 3 cut(s) 86, 706, 808
PpuMI RGGWCCY 1 cut(s) 799
Psp124BI GAGCTC 1 cut(s) 626
Psp5II RGGWCCY 1 cut(s) 799
Psp6I CCWGG 2 cut(s) 156, 239
PspGI CCWGG 2 cut(s) 156, 239
PspN4I GGNNCC 2 cut(s) 558, 768
PspPI GGNCC 3 cut(s) 154, 602, 799
PspPPI RGGWCCY 1 cut(s) 799
PspXI VCTCGAGB 1 cut(s) 484
PstNI CAGNNNCTG 1 cut(s) 663
PsuI RGATCY 2 cut(s) 556, 766
PsyI GACNNNGTC 1 cut(s) 311
PvuII CAGCTG 1 cut(s) 225
RsaI GTAC 1 cut(s) 743
RsaNI GTAC 1 cut(s) 742
SacI GAGCTC 1 cut(s) 626
SacII CCGCGG 1 cut(s) 391
SaqAI TTAA 1 cut(s) 832
SatI GCNGC 7 cut(s) 10, 13, 166, 226, 235, 391, 777
Sau3AI GATC 6 cut(s) 201, 259, 267, 514, 556, 766
Sau96I GGNCC 3 cut(s) 154, 602, 799
SchI GAGTC 3 cut(s) 86, 707, 808
ScrFI CCNGG 2 cut(s) 158, 241
SduI GDGCHC 1 cut(s) 626
SfaNI GCATC 1 cut(s) 718
Sfr274I CTCGAG 2 cut(s) 484, 695
Sfr303I CCGCGG 1 cut(s) 391
SgrBI CCGCGG 1 cut(s) 391
SinI GGWCC 2 cut(s) 602, 799
SlaI CTCGAG 2 cut(s) 484, 695
SmlI CTYRAG 3 cut(s) 302, 484, 695
SmoI CTYRAG 3 cut(s) 302, 484, 695
SphI GCATGC 1 cut(s) 718
Sse9I AATT 3 cut(s) 362, 381, 667
SsiI CCGC 8 cut(s) 12, 166, 178, 235, 324, 388, 390, 581
SspMI CTAG 2 cut(s) 405, 677
SstI GAGCTC 1 cut(s) 626
StyD4I CCNGG 2 cut(s) 156, 239
TaiI ACGT 1 cut(s) 312
TaqI TCGA 3 cut(s) 189, 485, 696
TasI AATT 3 cut(s) 362, 381, 667
TatI WGTACW 1 cut(s) 741
TauI GCSGC 4 cut(s) 15, 168, 237, 393
TfiI GAWTC 2 cut(s) 477, 502
Tru1I TTAA 1 cut(s) 832
Tru9I TTAA 1 cut(s) 832
TscAI CASTG 2 cut(s) 664, 739
TseI GCWGC 3 cut(s) 9, 225, 776
TspDTI ATGAA 1 cut(s) 152
TspGWI ACGGA 2 cut(s) 238, 415
TspRI CASTG 2 cut(s) 664, 739
Tth111I GACNNNGTC 1 cut(s) 311
VpaK11BI GGWCC 2 cut(s) 602, 799
XapI RAATTY 3 cut(s) 362, 381, 667
XceI RCATGY 1 cut(s) 718
XhoI CTCGAG 2 cut(s) 484, 695
XspI CTAG 2 cut(s) 405, 677
ZraI GACGTC 1 cut(s) 310
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.