MD12G1218500.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013410
Physical Location & Seq
Reverse (-)
29607904 .. 29609139
1236 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1218500.v1.1.491

Sequence Viewer

Length: 798 bp
ATGGGTTGCTGCGGGGACGAAGAAAACGACAACGACATCGTCAACCCTCTTTACTCCGACGCCGCCGCCCCAACCCTCGGCGACTCCTCCGACTCCACCACCACGATATCCCCTATGAACTCTCACTTCTCGGCCTTAACTTGCCGCGACACCCTCCGCCTCATCTTCGAGAAGCTCCCAATTCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGAGTCTGGAGCTCCGTCGCCTCCGATCAGGAGATCGTCGCCAAAGCCTTCAAAGCTCCTTGGAATCTGAAGGAGGTGATCGGAAAGCCGGCGTCTTCAAGCTTCTGGAGGGACAATTCGATCGGAAAATTCGCCATCTCGCACCGGATTGTCCGCGGGGACAGCGTCGCCAGCCTCGCCGTCAAGTATTCCGTTCAGGTGATGGACATAAAACGATTGAACAACATGATGAGTGAGCACGGAATATACTCTCGGGAGAGATTGCTGATTCCGGTAAGCAATGCGGAGATCCTTTCGGAAGCTACGTGCTATGTTGAAGTGGATAATTATGCTAAGAGAGAGGTGGCTGTGTTGTATTTGGAGGGCGGCCCCGACCCGAAAACCATAAACAGCGGCAGCATCTCTTCGTGCTCTGTTTTGCAGAGCAAGAAAAGAGTGGTTGAATCACTGAGGAGAAGCCTGCGAGTGGATGATGCAACTGCAAGCTACTACTTGTCCATTGCCGATGGCGATCCAAGAGCTGCAATCTCTCAGTTCTCCCAAGACCTTAGGTGGGAGACCAACACCCACCTCGGCTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

266

Amino Acids

29.08

Weight (kDa)

5.65

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 49 - 83 3.3e-06 F-box domain
F-box-like PF12937 52 - 82 4.5e-06 F-box-like
LysM PF01476 119 - 162 2e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 147, 369
AclWI GGATC 2 cut(s) 496, 719
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 302
AcyI GRCGYC 2 cut(s) 60, 305
AfiI CCNNNNNNNGG 3 cut(s) 77, 679, 766
AgsI TTSAA 5 cut(s) 265, 312, 433, 530, 656
AluBI AGCT 8 cut(s) 175, 204, 225, 269, 315, 515, 699, 734
AluI AGCT 8 cut(s) 175, 204, 225, 269, 315, 515, 699, 734
Alw21I GWGCWC 3 cut(s) 227, 453, 626
Alw26I GTCTC 1 cut(s) 764
AlwI GGATC 2 cut(s) 496, 719
Ama87I CYCGRG 1 cut(s) 465
AoxI GGCC 2 cut(s) 132, 580
ApeKI GCWGC 4 cut(s) 9, 204, 609, 734
ApoI RAATTY 1 cut(s) 341
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 301, 424
AvaI CYCGRG 1 cut(s) 465
AxyI CCTNAGG 1 cut(s) 761
BanII GRGCYC 1 cut(s) 227
BbsI GAAGAC 1 cut(s) 300
Bbv12I GWGCWC 3 cut(s) 227, 453, 626
BbvI GCAGC 3 cut(s) 191, 621, 721
BccI CCATC 3 cut(s) 356, 409, 713
BceAI ACGGC 1 cut(s) 377
BcoDI GTCTC 1 cut(s) 764
BisI GCNGC 9 cut(s) 10, 63, 66, 145, 205, 580, 607, 610, 735
BlsI GCNGC 9 cut(s) 11, 64, 67, 146, 206, 581, 608, 611, 736
BmeT110I CYCGRG 1 cut(s) 465
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 1 cut(s) 583
BmsI GCATC 2 cut(s) 621, 676
BpiI GAAGAC 1 cut(s) 300
BpmI CTGGAG 3 cut(s) 184, 241, 340
BsaAI YACGTR 1 cut(s) 519
BsaBI GATNNNNATC 1 cut(s) 723
BsaHI GRCGYC 2 cut(s) 60, 305
BsaI GGTCTC 1 cut(s) 764
BsaJI CCNNGG 4 cut(s) 76, 272, 367, 784
BsaWI WCCGGW 2 cut(s) 357, 484
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 679, 766
Bse118I RCCGGY 1 cut(s) 301
Bse21I CCTNAGG 1 cut(s) 761
Bse3DI GCAATG 2 cut(s) 499, 711
Bse8I GATNNNNATC 1 cut(s) 723
BseDI CCNNGG 4 cut(s) 76, 272, 367, 784
BseGI GGATG 1 cut(s) 688
BseJI GATNNNNATC 1 cut(s) 723
BseLI CCNNNNNNNGG 3 cut(s) 77, 679, 766
BseMI GCAATG 2 cut(s) 499, 711
BseMII CTCAG 2 cut(s) 653, 758
BseRI GAGGAG 2 cut(s) 76, 679
BseXI GCAGC 3 cut(s) 191, 621, 721
Bsh1236I CGCG 2 cut(s) 147, 369
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 2 cut(s) 134, 582
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 3 cut(s) 227, 453, 626
BsiHKCI CYCGRG 1 cut(s) 465
BsiSI CCGG 3 cut(s) 302, 358, 485
BslFI GGGAC 3 cut(s) 29, 338, 386
BslI CCNNNNNNNGG 3 cut(s) 77, 679, 766
BsmAI GTCTC 1 cut(s) 764
BsmFI GGGAC 3 cut(s) 29, 338, 386
BsnI GGCC 2 cut(s) 134, 582
Bso31I GGTCTC 1 cut(s) 764
BsoBI CYCGRG 1 cut(s) 465
Bsp1286I GDGCHC 3 cut(s) 227, 453, 626
Bsp143I GATC 6 cut(s) 238, 246, 291, 333, 501, 724
BspANI GGCC 2 cut(s) 134, 582
BspCNI CTCAG 2 cut(s) 654, 757
BspFNI CGCG 2 cut(s) 147, 369
BspLI GGNNCC 1 cut(s) 583
BspPI GGATC 2 cut(s) 496, 719
BspTNI GGTCTC 1 cut(s) 764
BsrDI GCAATG 2 cut(s) 499, 711
BsrFI RCCGGY 1 cut(s) 301
BssAI RCCGGY 1 cut(s) 301
BssECI CCNNGG 4 cut(s) 76, 272, 367, 784
BssMI GATC 6 cut(s) 238, 246, 291, 333, 501, 724
BssNI GRCGYC 2 cut(s) 60, 305
BssT1I CCWWGG 1 cut(s) 272
Bst6I CTCTTC 1 cut(s) 622
BstACI GRCGYC 2 cut(s) 60, 305
BstBAI YACGTR 1 cut(s) 519
BstC8I GCNNGC 5 cut(s) 196, 303, 385, 674, 697
BstDEI CTNAG 5 cut(s) 546, 662, 744, 761, 795
BstDSI CCRYGG 1 cut(s) 367
BstF5I GGATG 1 cut(s) 688
BstFNI CGCG 2 cut(s) 147, 369
BstKTI GATC 6 cut(s) 241, 249, 294, 336, 504, 727
BstMAI GTCTC 1 cut(s) 764
BstMBI GATC 6 cut(s) 238, 246, 291, 333, 501, 724
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 6 cut(s) 204, 210, 266, 375, 384, 389
BstUI CGCG 2 cut(s) 147, 369
BstV1I GCAGC 3 cut(s) 191, 621, 721
BstV2I GAAGAC 1 cut(s) 300
BstX2I RGATCY 1 cut(s) 501
BstYI RGATCY 1 cut(s) 501
Bsu36I CCTNAGG 1 cut(s) 761
BsuRI GGCC 2 cut(s) 134, 582
BtgI CCRYGG 1 cut(s) 367
BtsCI GGATG 1 cut(s) 688
BtsIMutI CAGTG 1 cut(s) 659
Cac8I GCNNGC 5 cut(s) 196, 303, 385, 674, 697
Cfr10I RCCGGY 1 cut(s) 301
Cfr13I GGNCC 1 cut(s) 581
Cfr42I CCGCGG 1 cut(s) 370
CseI GACGC 4 cut(s) 68, 196, 294, 367
CviAII CATG 1 cut(s) 439
DdeI CTNAG 5 cut(s) 546, 662, 744, 761, 795
DpnI GATC 6 cut(s) 240, 248, 293, 335, 503, 726
DpnII GATC 6 cut(s) 238, 246, 291, 333, 501, 724
Eam1104I CTCTTC 1 cut(s) 622
EarI CTCTTC 1 cut(s) 622
EciI GGCGGA 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 225
Eco130I CCWWGG 1 cut(s) 272
Eco24I GRGCYC 1 cut(s) 227
Eco31I GGTCTC 1 cut(s) 764
Eco32I GATATC 1 cut(s) 108
Eco53kI GAGCTC 1 cut(s) 225
Eco57I CTGAAG 1 cut(s) 302
Eco81I CCTNAGG 1 cut(s) 761
Eco88I CYCGRG 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 225
EcoRV GATATC 1 cut(s) 108
EcoT14I CCWWGG 1 cut(s) 272
EcoT38I GRGCYC 1 cut(s) 227
ErhI CCWWGG 1 cut(s) 272
FaeI CATG 1 cut(s) 442
FaiI YATR 7 cut(s) 116, 422, 440, 460, 525, 543, 599
FaqI GGGAC 3 cut(s) 29, 338, 386
FatI CATG 1 cut(s) 438
FauI CCCGC 2 cut(s) 5, 362
Fnu4HI GCNGC 9 cut(s) 10, 63, 66, 145, 205, 580, 607, 610, 735
FokI GGATG 1 cut(s) 695
FriOI GRGCYC 1 cut(s) 227
Fsp4HI GCNGC 9 cut(s) 10, 63, 66, 145, 205, 580, 607, 610, 735
GluI GCNGC 9 cut(s) 10, 63, 66, 145, 205, 580, 607, 610, 735
GsuI CTGGAG 3 cut(s) 184, 241, 340
HaeIII GGCC 2 cut(s) 134, 582
HapII CCGG 3 cut(s) 302, 358, 485
HgaI GACGC 4 cut(s) 68, 196, 294, 367
Hin1I GRCGYC 2 cut(s) 60, 305
Hin1II CATG 1 cut(s) 442
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HindIII AAGCTT 1 cut(s) 313
HinfI GANTC 6 cut(s) 83, 92, 216, 277, 481, 656
HpaII CCGG 3 cut(s) 302, 358, 485
HphI GGTGA 2 cut(s) 301, 424
Hpy166II GTNNAC 1 cut(s) 43
Hpy188I TCNGA 7 cut(s) 58, 91, 238, 282, 296, 338, 511
Hpy188III TCNNGA 6 cut(s) 169, 185, 220, 242, 319, 467
Hpy8I GTNNAC 1 cut(s) 43
Hpy99I CGWCG 4 cut(s) 62, 233, 254, 383
HpyAV CCTTC 2 cut(s) 271, 277
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 4 cut(s) 634, 689, 695, 737
HpyF10VI GCNNNNNNNGC 6 cut(s) 204, 210, 266, 375, 384, 389
HpyF3I CTNAG 5 cut(s) 546, 662, 744, 761, 795
HpySE526I ACGT 1 cut(s) 518
Hsp92I GRCGYC 2 cut(s) 60, 305
Hsp92II CATG 1 cut(s) 442
KroI GCCGGC 1 cut(s) 301
KroNI GCCGGC 1 cut(s) 303
KspI CCGCGG 1 cut(s) 370
Kzo9I GATC 6 cut(s) 238, 246, 291, 333, 501, 724
LmnI GCTCC 5 cut(s) 180, 203, 222, 230, 274
Lsp1109I GCAGC 3 cut(s) 191, 621, 721
LweI GCATC 2 cut(s) 621, 676
MaeII ACGT 1 cut(s) 518
MalI GATC 6 cut(s) 240, 248, 293, 335, 503, 726
MboI GATC 6 cut(s) 238, 246, 291, 333, 501, 724
MboII GAAGA 4 cut(s) 32, 157, 300, 609
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 3 cut(s) 227, 453, 626
MluCI AATT 4 cut(s) 180, 328, 341, 538
MlyI GAGTC 3 cut(s) 77, 86, 225
MmeI TCCRAC 2 cut(s) 81, 114
MroNI GCCGGC 1 cut(s) 301
MseI TTAA 1 cut(s) 137
MspA1I CMGCKG 3 cut(s) 204, 369, 606
MspI CCGG 3 cut(s) 302, 358, 485
MvnI CGCG 2 cut(s) 147, 369
MwoI GCNNNNNNNGC 6 cut(s) 204, 210, 266, 375, 384, 389
NaeI GCCGGC 1 cut(s) 303
NdeII GATC 6 cut(s) 238, 246, 291, 333, 501, 724
NgoMIV GCCGGC 1 cut(s) 301
NlaIII CATG 1 cut(s) 442
NlaIV GGNNCC 1 cut(s) 583
NmeAIII GCCGAG 4 cut(s) 57, 110, 239, 765
PcsI WCGNNNNNNNCGW 2 cut(s) 24, 515
PdiI GCCGGC 1 cut(s) 303
PfeI GAWTC 3 cut(s) 277, 481, 656
PflFI GACNNNGTC 2 cut(s) 38, 377
PkrI GCNGC 9 cut(s) 11, 64, 67, 146, 206, 581, 608, 611, 736
Ple19I CGATCG 1 cut(s) 336
PleI GAGTC 3 cut(s) 77, 86, 224
PpsI GAGTC 3 cut(s) 77, 86, 224
Ppu21I YACGTR 1 cut(s) 519
Psp124BI GAGCTC 1 cut(s) 227
PspN4I GGNNCC 1 cut(s) 583
PspPI GGNCC 1 cut(s) 581
PsuI RGATCY 1 cut(s) 501
PsyI GACNNNGTC 2 cut(s) 38, 377
PvuI CGATCG 1 cut(s) 336
PvuII CAGCTG 1 cut(s) 204
SacI GAGCTC 1 cut(s) 227
SacII CCGCGG 1 cut(s) 370
SaqAI TTAA 1 cut(s) 137
SatI GCNGC 9 cut(s) 10, 63, 66, 145, 205, 580, 607, 610, 735
Sau3AI GATC 6 cut(s) 238, 246, 291, 333, 501, 724
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 3 cut(s) 77, 86, 225
SduI GDGCHC 3 cut(s) 227, 453, 626
SfaNI GCATC 2 cut(s) 621, 676
Sfr303I CCGCGG 1 cut(s) 370
SgrBI CCGCGG 1 cut(s) 370
Sse9I AATT 4 cut(s) 180, 328, 341, 538
SstI GAGCTC 1 cut(s) 227
StyI CCWWGG 1 cut(s) 272
TaiI ACGT 1 cut(s) 521
TaqI TCGA 2 cut(s) 168, 332
TasI AATT 4 cut(s) 180, 328, 341, 538
TauI GCSGC 5 cut(s) 65, 68, 147, 582, 609
TfiI GAWTC 3 cut(s) 277, 481, 656
Tru1I TTAA 1 cut(s) 137
Tru9I TTAA 1 cut(s) 137
TscAI CASTG 1 cut(s) 666
TseI GCWGC 4 cut(s) 9, 204, 609, 734
TspDTI ATGAA 1 cut(s) 131
TspGWI ACGGA 3 cut(s) 217, 394, 468
TspRI CASTG 1 cut(s) 666
Tth111I GACNNNGTC 2 cut(s) 38, 377
XapI RAATTY 1 cut(s) 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.