Rroxscaffold_6G00424630

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013410
Physical Location & Seq
Reverse (-)
45776110 .. 45777426
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00424630.1

Sequence Viewer

Length: 837 bp
ATGGGTTGCTGCGGCGACGAAGAAAACGACGACGATATTCTCAACCCTCTCAGCCCCACCTCCTCCACCACCACCACCACCCAAACCCCAGACGACTCGTCGTCCTCCTCCTCCGACGGCTACACCACCATATCCCTGATGAACTCTCACTTCTCGGCCCTGGCCTGCCGCGACACCCTCCGCCTCATCTTCGAGAAGCTTCCGATCCCCGACCTCGCTCGCTCCAGCTGCGTCTGCCGCGCCTGGAACTCCGTCGCCTCCGATCAAGAGATCGTCGCCAACGCCTTCAAATCGCCGTGGAAGCTCAAGGACGTCGTCGGAAAACCCGCCTCCGGAAGCTTCTGGAGAGATGACACCCTCGGGAAATTCGCCATCTCGCACCGAATTGCCCGCGGCGATAGCGTCGCTAGCCTCGCCGTTAAGTATTCCGTTCAGGTGATGGACATAAAGCGCTTGAACAACATGATGAGCGAGCATGGAATATACTCGAGGGAGAGGTTGTTGATTCCGATAAGCGATCCTGAAATGCTTGTGGGAGCAACCTGCTATGTCGAAGTGGATCCCTGTGCTAAGAGAGAAGTTGCGGTGTTGTATTTGGAAGATGGTCCTGACCAGAAATCAATGAGCTCTTGCTCTTTGTCGCAGAAGAGTGGCGACAGCAGTGGCCCGAATTCTGAACTAGGTAAGAAGAGGGTTCTTGAGTCTCTGAGGAGAAGCATGCACGTTGATGATGCCACTGCTCTGTACTACTTGTCCATTGCTGATGGGGATCCGAGAGCTGCATTGTCTCAGTTCTCGCAGGACCTTAGGTGGGAGACTCATTCGGCCTTTGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like LysM

Protein Analysis

278

Amino Acids

30.41

Weight (kDa)

5.09

Isoelectric Point (pI)

43.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 57 - 91 1.8e-07 F-box domain
F-box-like PF12937 60 - 90 1.9e-07 F-box-like
LysM PF01476 127 - 170 3.7e-06 LysM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013410)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G55000 AT1G55000 AT1G55000
fragaria_vesca FvH4_6g06020
malus_domestica MD04G1204800.v1.1 MD12G1218500.v1.1
prunus_persica Prupe.6G324800_v2.0.a1
pyrus_communis pycom04g18100
rosa_chinensis RchiOBHm_Chr3g0454981
rosa_laevigata RLG00000025396
rosa_multiflora Rmu_sc0001861.1_g000058
rosa_roxburghii Rroxscaffold_6G00424630
rosa_rugosa Rorug03G0003700
rosa_samantha Rh3AG063300 Rh3BG065300 Rh3CG064100 Rh3DG065300
rosa_wichuraiana Rw3G004970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 315
Acc36I ACCTGC 1 cut(s) 551
AccII CGCG 3 cut(s) 171, 240, 393
AccIII TCCGGA 1 cut(s) 332
AciI CCGC 8 cut(s) 12, 169, 181, 238, 327, 391, 393, 584
AclWI GGATC 6 cut(s) 199, 512, 554, 567, 764, 777
AcsI RAATTY 2 cut(s) 365, 670
AcyI GRCGYC 1 cut(s) 312
AfaI GTAC 1 cut(s) 746
AfeI AGCGCT 1 cut(s) 452
AfiI CCNNNNNNNGG 2 cut(s) 332, 811
AgsI TTSAA 2 cut(s) 289, 457
AhdI GACNNNNNGTC 2 cut(s) 97, 100
AjnI CCWGG 2 cut(s) 159, 242
AluBI AGCT 6 cut(s) 199, 228, 304, 339, 627, 779
AluI AGCT 6 cut(s) 199, 228, 304, 339, 627, 779
Alw21I GWGCWC 1 cut(s) 629
Alw26I GTCTC 3 cut(s) 708, 792, 809
AlwI GGATC 6 cut(s) 199, 512, 554, 567, 764, 777
Ama87I CYCGRG 2 cut(s) 359, 487
Aor13HI TCCGGA 1 cut(s) 332
Aor51HI AGCGCT 1 cut(s) 452
AoxI GGCC 4 cut(s) 156, 162, 664, 825
ApeKI GCWGC 3 cut(s) 9, 228, 779
ApoI RAATTY 2 cut(s) 365, 670
AspLEI GCGC 2 cut(s) 242, 453
AspS9I GGNCC 4 cut(s) 157, 605, 665, 802
AsuHPI GGTGA 1 cut(s) 448
AsuNHI GCTAGC 1 cut(s) 407
AvaI CYCGRG 2 cut(s) 359, 487
AvaII GGWCC 2 cut(s) 605, 802
AxyI CCTNAGG 1 cut(s) 806
BamHI GGATCC 2 cut(s) 559, 769
BanII GRGCYC 1 cut(s) 629
Bbv12I GWGCWC 1 cut(s) 629
BbvI GCAGC 2 cut(s) 215, 766
BccI CCATC 4 cut(s) 380, 433, 596, 758
BceAI ACGGC 3 cut(s) 133, 280, 401
BciT130I CCWGG 2 cut(s) 161, 244
BcoDI GTCTC 3 cut(s) 708, 792, 809
BfaI CTAG 2 cut(s) 408, 680
BfoI RGCGCY 1 cut(s) 454
BfuAI ACCTGC 1 cut(s) 551
BisI GCNGC 7 cut(s) 10, 13, 169, 229, 238, 394, 780
BlsI GCNGC 7 cut(s) 11, 14, 170, 230, 239, 395, 781
Bme1390I CCNGG 2 cut(s) 161, 244
Bme18I GGWCC 2 cut(s) 605, 802
BmeRI GACNNNNNGTC 2 cut(s) 97, 100
BmeT110I CYCGRG 2 cut(s) 359, 487
BmgT120I GGNCC 4 cut(s) 157, 605, 665, 802
BmiI GGNNCC 2 cut(s) 561, 771
BmrFI CCNGG 2 cut(s) 161, 244
BmsI GCATC 1 cut(s) 721
BmtI GCTAGC 1 cut(s) 411
BpmI CTGGAG 2 cut(s) 208, 364
BpuEI CTTGAG 2 cut(s) 290, 719
BsaBI GATNNNNATC 1 cut(s) 768
BsaHI GRCGYC 1 cut(s) 312
BsaJI CCNNGG 4 cut(s) 159, 296, 358, 391
BsaWI WCCGGW 1 cut(s) 332
BsaXI ACNNNNNCTCC 2 cut(s) 485, 515
Bsc4I CCNNNNNNNGG 2 cut(s) 332, 811
Bse21I CCTNAGG 1 cut(s) 806
Bse3DI GCAATG 1 cut(s) 756
Bse8I GATNNNNATC 1 cut(s) 768
BseAI TCCGGA 1 cut(s) 332
BseBI CCWGG 2 cut(s) 161, 244
BseDI CCNNGG 4 cut(s) 159, 296, 358, 391
BseJI GATNNNNATC 1 cut(s) 768
BseLI CCNNNNNNNGG 2 cut(s) 332, 811
BseMI GCAATG 1 cut(s) 756
BseMII CTCAG 3 cut(s) 64, 698, 803
BseRI GAGGAG 4 cut(s) 52, 97, 100, 724
BseXI GCAGC 2 cut(s) 215, 766
Bsh1236I CGCG 3 cut(s) 171, 240, 393
BshFI GGCC 4 cut(s) 158, 164, 666, 827
BsiHKAI GWGCWC 1 cut(s) 629
BsiHKCI CYCGRG 2 cut(s) 359, 487
BsiSI CCGG 1 cut(s) 333
BslI CCNNNNNNNGG 2 cut(s) 332, 811
BsmAI GTCTC 3 cut(s) 708, 792, 809
BsnI GGCC 4 cut(s) 158, 164, 666, 827
BsoBI CYCGRG 2 cut(s) 359, 487
Bsp1286I GDGCHC 1 cut(s) 629
Bsp13I TCCGGA 1 cut(s) 332
Bsp143I GATC 6 cut(s) 204, 262, 270, 517, 559, 769
BspACI CCGC 8 cut(s) 12, 169, 181, 238, 327, 391, 393, 584
BspANI GGCC 4 cut(s) 158, 164, 666, 827
BspCNI CTCAG 3 cut(s) 63, 699, 802
BspEI TCCGGA 1 cut(s) 332
BspFNI CGCG 3 cut(s) 171, 240, 393
BspLI GGNNCC 2 cut(s) 561, 771
BspMI ACCTGC 1 cut(s) 551
BspOI GCTAGC 1 cut(s) 411
BspPI GGATC 6 cut(s) 199, 512, 554, 567, 764, 777
BsrDI GCAATG 1 cut(s) 756
BssECI CCNNGG 4 cut(s) 159, 296, 358, 391
BssMI GATC 6 cut(s) 204, 262, 270, 517, 559, 769
BssNI GRCGYC 1 cut(s) 312
Bst2UI CCWGG 2 cut(s) 161, 244
Bst6I CTCTTC 2 cut(s) 641, 683
BstACI GRCGYC 1 cut(s) 312
BstC8I GCNNGC 6 cut(s) 166, 220, 391, 409, 473, 719
BstDEI CTNAG 5 cut(s) 50, 570, 707, 789, 806
BstDSI CCRYGG 2 cut(s) 296, 391
BstFNI CGCG 3 cut(s) 171, 240, 393
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 2 cut(s) 242, 453
BstKTI GATC 6 cut(s) 207, 265, 273, 520, 562, 772
BstMAI GTCTC 3 cut(s) 708, 792, 809
BstMBI GATC 6 cut(s) 204, 262, 270, 517, 559, 769
BstMWI GCNNNNNNNGC 7 cut(s) 228, 234, 237, 301, 399, 408, 413
BstNI CCWGG 2 cut(s) 161, 244
BstNSI RCATGY 1 cut(s) 721
BstSCI CCNGG 2 cut(s) 159, 242
BstUI CGCG 3 cut(s) 171, 240, 393
BstV1I GCAGC 2 cut(s) 215, 766
BstX2I RGATCY 2 cut(s) 559, 769
BstYI RGATCY 2 cut(s) 559, 769
Bsu36I CCTNAGG 1 cut(s) 806
BsuRI GGCC 4 cut(s) 158, 164, 666, 827
BtgI CCRYGG 2 cut(s) 296, 391
BtsI GCAGTG 2 cut(s) 667, 735
BtsIMutI CAGTG 2 cut(s) 667, 735
BveI ACCTGC 1 cut(s) 551
Cac8I GCNNGC 6 cut(s) 166, 220, 391, 409, 473, 719
CfoI GCGC 2 cut(s) 242, 453
Cfr13I GGNCC 4 cut(s) 157, 605, 665, 802
Cfr42I CCGCGG 1 cut(s) 394
CseI GACGC 2 cut(s) 220, 391
Csp6I GTAC 1 cut(s) 745
CviAII CATG 3 cut(s) 463, 476, 718
CviQI GTAC 1 cut(s) 745
DdeI CTNAG 5 cut(s) 50, 570, 707, 789, 806
DpnI GATC 6 cut(s) 206, 264, 272, 519, 561, 771
DpnII GATC 6 cut(s) 204, 262, 270, 517, 559, 769
DriI GACNNNNNGTC 2 cut(s) 97, 100
Eam1104I CTCTTC 2 cut(s) 641, 683
Eam1105I GACNNNNNGTC 2 cut(s) 97, 100
EarI CTCTTC 2 cut(s) 641, 683
EciI GGCGGA 1 cut(s) 170
Ecl136II GAGCTC 1 cut(s) 627
Eco24I GRGCYC 1 cut(s) 629
Eco47I GGWCC 2 cut(s) 605, 802
Eco47III AGCGCT 1 cut(s) 452
Eco53kI GAGCTC 1 cut(s) 627
Eco81I CCTNAGG 1 cut(s) 806
Eco88I CYCGRG 2 cut(s) 359, 487
EcoICRI GAGCTC 1 cut(s) 627
EcoO109I RGGNCCY 1 cut(s) 802
EcoRI GAATTC 1 cut(s) 670
EcoRII CCWGG 2 cut(s) 159, 242
EcoT38I GRGCYC 1 cut(s) 629
FaeI CATG 3 cut(s) 466, 479, 721
FaiI YATR 7 cut(s) 131, 446, 464, 477, 484, 549, 719
FatI CATG 3 cut(s) 462, 475, 717
FauI CCCGC 2 cut(s) 334, 398
Fnu4HI GCNGC 7 cut(s) 10, 13, 169, 229, 238, 394, 780
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 7 cut(s) 10, 13, 169, 229, 238, 394, 780
FspBI CTAG 2 cut(s) 408, 680
GlaI GCGC 2 cut(s) 241, 452
GluI GCNGC 7 cut(s) 10, 13, 169, 229, 238, 394, 780
GsuI CTGGAG 2 cut(s) 208, 364
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 4 cut(s) 158, 164, 666, 827
HapII CCGG 1 cut(s) 333
HgaI GACGC 2 cut(s) 220, 391
HhaI GCGC 2 cut(s) 242, 453
Hin1I GRCGYC 1 cut(s) 312
Hin1II CATG 3 cut(s) 466, 479, 721
Hin6I GCGC 2 cut(s) 240, 451
HinP1I GCGC 2 cut(s) 240, 451
HindIII AAGCTT 2 cut(s) 197, 337
HinfI GANTC 4 cut(s) 95, 505, 701, 817
HpaII CCGG 1 cut(s) 333
HphI GGTGA 1 cut(s) 448
Hpy188I TCNGA 8 cut(s) 115, 204, 262, 320, 510, 676, 708, 774
Hpy188III TCNNGA 8 cut(s) 193, 266, 333, 343, 361, 521, 608, 698
HpyAV CCTTC 1 cut(s) 295
HpyCH4IV ACGT 2 cut(s) 312, 723
HpyCH4V TGCA 2 cut(s) 721, 782
HpyF10VI GCNNNNNNNGC 7 cut(s) 228, 234, 237, 301, 399, 408, 413
HpyF3I CTNAG 5 cut(s) 50, 570, 707, 789, 806
HpySE526I ACGT 2 cut(s) 312, 723
Hsp92I GRCGYC 1 cut(s) 312
Hsp92II CATG 3 cut(s) 466, 479, 721
HspAI GCGC 2 cut(s) 240, 451
Kpn2I TCCGGA 1 cut(s) 332
KspI CCGCGG 1 cut(s) 394
Kzo9I GATC 6 cut(s) 204, 262, 270, 517, 559, 769
LmnI GCTCC 2 cut(s) 227, 536
Lsp1109I GCAGC 2 cut(s) 215, 766
LweI GCATC 1 cut(s) 721
MaeI CTAG 2 cut(s) 408, 680
MaeII ACGT 2 cut(s) 312, 723
MalI GATC 6 cut(s) 206, 264, 272, 519, 561, 771
MboI GATC 6 cut(s) 204, 262, 270, 517, 559, 769
MboII GAAGA 5 cut(s) 32, 181, 611, 658, 700
MflI RGATCY 2 cut(s) 559, 769
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 3 cut(s) 365, 384, 670
MlyI GAGTC 3 cut(s) 89, 710, 811
MmeI TCCRAC 2 cut(s) 138, 298
MroI TCCGGA 1 cut(s) 332
MseI TTAA 2 cut(s) 420, 835
MslI CAYNNNNRTG 1 cut(s) 726
MspA1I CMGCKG 2 cut(s) 228, 393
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 2 cut(s) 161, 244
MvaI CCWGG 2 cut(s) 161, 244
MvnI CGCG 3 cut(s) 171, 240, 393
MwoI GCNNNNNNNGC 7 cut(s) 228, 234, 237, 301, 399, 408, 413
NdeII GATC 6 cut(s) 204, 262, 270, 517, 559, 769
NheI GCTAGC 1 cut(s) 407
NlaIII CATG 3 cut(s) 466, 479, 721
NlaIV GGNNCC 2 cut(s) 561, 771
NmeAIII GCCGAG 1 cut(s) 134
NspI RCATGY 1 cut(s) 721
PaeI GCATGC 1 cut(s) 721
PaeR7I CTCGAG 1 cut(s) 487
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 1 cut(s) 505
PflFI GACNNNGTC 1 cut(s) 314
PkrI GCNGC 7 cut(s) 11, 14, 170, 230, 239, 395, 781
PleI GAGTC 3 cut(s) 89, 709, 811
PpsI GAGTC 3 cut(s) 89, 709, 811
PpuMI RGGWCCY 1 cut(s) 802
Psp124BI GAGCTC 1 cut(s) 629
Psp5II RGGWCCY 1 cut(s) 802
Psp6I CCWGG 2 cut(s) 159, 242
PspGI CCWGG 2 cut(s) 159, 242
PspN4I GGNNCC 2 cut(s) 561, 771
PspPI GGNCC 4 cut(s) 157, 605, 665, 802
PspPPI RGGWCCY 1 cut(s) 802
PspXI VCTCGAGB 1 cut(s) 487
PsuI RGATCY 2 cut(s) 559, 769
PsyI GACNNNGTC 1 cut(s) 314
PvuII CAGCTG 1 cut(s) 228
RsaI GTAC 1 cut(s) 746
RsaNI GTAC 1 cut(s) 745
RseI CAYNNNNRTG 1 cut(s) 726
SacI GAGCTC 1 cut(s) 629
SacII CCGCGG 1 cut(s) 394
SaqAI TTAA 2 cut(s) 420, 835
SatI GCNGC 7 cut(s) 10, 13, 169, 229, 238, 394, 780
Sau3AI GATC 6 cut(s) 204, 262, 270, 517, 559, 769
Sau96I GGNCC 4 cut(s) 157, 605, 665, 802
SchI GAGTC 3 cut(s) 89, 710, 811
ScrFI CCNGG 2 cut(s) 161, 244
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 1 cut(s) 721
Sfr274I CTCGAG 1 cut(s) 487
Sfr303I CCGCGG 1 cut(s) 394
SgrBI CCGCGG 1 cut(s) 394
SinI GGWCC 2 cut(s) 605, 802
SlaI CTCGAG 1 cut(s) 487
SmiMI CAYNNNNRTG 1 cut(s) 726
SmlI CTYRAG 3 cut(s) 305, 487, 698
SmoI CTYRAG 3 cut(s) 305, 487, 698
SphI GCATGC 1 cut(s) 721
Sse9I AATT 3 cut(s) 365, 384, 670
SsiI CCGC 8 cut(s) 12, 169, 181, 238, 327, 391, 393, 584
SspMI CTAG 2 cut(s) 408, 680
SstI GAGCTC 1 cut(s) 629
StyD4I CCNGG 2 cut(s) 159, 242
TaiI ACGT 2 cut(s) 315, 726
TaqI TCGA 3 cut(s) 192, 488, 552
TasI AATT 3 cut(s) 365, 384, 670
TatI WGTACW 1 cut(s) 744
TauI GCSGC 4 cut(s) 15, 171, 240, 396
TfiI GAWTC 1 cut(s) 505
Tru1I TTAA 2 cut(s) 420, 835
Tru9I TTAA 2 cut(s) 420, 835
TscAI CASTG 2 cut(s) 667, 742
TseI GCWGC 3 cut(s) 9, 228, 779
TspDTI ATGAA 1 cut(s) 155
TspGWI ACGGA 2 cut(s) 241, 418
TspRI CASTG 2 cut(s) 667, 742
Tth111I GACNNNGTC 1 cut(s) 314
VpaK11BI GGWCC 2 cut(s) 605, 802
XapI RAATTY 2 cut(s) 365, 670
XceI RCATGY 1 cut(s) 721
XhoI CTCGAG 1 cut(s) 487
XspI CTAG 2 cut(s) 408, 680
ZraI GACGTC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.