MD04G1217100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013395
Physical Location & Seq
Forward (+)
29975594 .. 29978565
2972 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1217100.v1.1.491

Sequence Viewer

Length: 1011 bp
ATGGCTCTGTGGTCGTCACCGTCAACGTCGTCGTCGCCGAAGGGGATAGTGATAACGGTGCCGGCGCTGGTCCTCACGGCGTCAGTTGCCGCAGTTTTCTTCTTCTTTCTCTTGTTCTCTTTATCGTCGCCGTGCACTTGCGGCGGCGGTGCTGGTATTGCGCGTGTGGGTTATGTCGGTGGACCCAGTGTCGGCGTGGCGGAGGGCGGAGGAGGAGAGACGATATGGTCGTCGAAGGAGGATGTGGAGTGGGTGAAGGATCAGATCCGAGTGAATGGGTTGCATATGCAGGATAACGTGTTGAGGAAGGGAATTAACCCTCGCACCAGAGCTCAGCAGCTTGAAGATCTCTTGCAATTCAAGGGTATATCTCACTATGAAGGACCTGATTCGGAAAATCATACTGCCCTCCCATGCCCTGGTGAGCTCCTAACCGAGGAGCACCACAGTAACTACGGTGAGCCTTGGGCAGGTGGGCGAGATGTTTTTGAGTTCCTCGCTCAGTCCTCTCATCTGAAGCCTGACTCCAATGTCCTTGAGATCGGTTGTGGTACCCTCCGAGTTGGTTTGCATTTCATTCGCTATCTTAATCCCGGACACTTCCACTGCCTTGAAAGAGATGAGCTCTCGCTAATGGCTGCCTTCAGATACGAGCTTCCTTCCCAAGGTCTTCTGCACAAGCGCCCTTTGATTGTGAAAGGCGACAACATGGACTTCAACAGATTTGGGTCCGATGTTGTGTATGACTTGATCTATGCTAGCGCTGTGTTTCTTCATATGCCTGATAAGCTTGTGTGGGTTGGATTAGAGAGATTGACAAATAAACTGAAACCCTATGACGGGCGAATCTTCGTTTCCCATAATATCAAATTCTGTTCGCGGTTGGGAGGAGACGAGTGTACAAAGCAGCTCACGAGTTTAGGCCTGGAGTATGTCGGAAAGCATACACATGACAGCTTGCTATTTAATCACTACGAAATCTGGTTCGAGTTTAGGCGGACAAGGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_23

Protein Analysis

337

Amino Acids

37.4

Weight (kDa)

6.31

Isoelectric Point (pI)

40.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 179 - 281 1.6e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05100
fragaria_vesca FvH4_6g03940
malus_domestica MD04G1217100.v1.1 MD12G1234100.v1.1
prunus_persica Prupe.6G337200_v2.0.a1 Prupe.6G337200_v2.0.a1
pyrus_communis pycom04g19320 pycom12g21650 pycom16g23200
rosa_chinensis RchiOBHm_Chr3g0452511
rosa_laevigata RLG00000025596
rosa_roxburghii Rroxscaffold_6G00429540
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043200 Rh3BG044900 Rh3CG043500 Rh3DG044300
rosa_wichuraiana Rw3G003350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 461
AasI GACNNNNNNGTC 2 cut(s) 226, 530
Acc36I ACCTGC 1 cut(s) 461
Acc65I GGTACC 1 cut(s) 551
AccB1I GGYRCC 2 cut(s) 58, 551
AccB7I CCANNNNNTGG 1 cut(s) 419
AccII CGCG 2 cut(s) 163, 880
AciI CCGC 8 cut(s) 90, 141, 144, 147, 200, 207, 880, 997
AclWI GGATC 2 cut(s) 259, 267
AcsI RAATTY 1 cut(s) 869
AcuI CTGAAG 2 cut(s) 536, 628
AcyI GRCGYC 1 cut(s) 80
AfaI GTAC 2 cut(s) 553, 901
AfeI AGCGCT 1 cut(s) 763
AfiI CCNNNNNNNGG 7 cut(s) 191, 419, 436, 470, 665, 839, 840
AflIII ACRYGT 1 cut(s) 297
AgsI TTSAA 4 cut(s) 344, 361, 614, 718
AhdI GACNNNNNGTC 1 cut(s) 188
AjnI CCWGG 2 cut(s) 418, 924
AluBI AGCT 8 cut(s) 332, 340, 427, 625, 655, 790, 910, 957
AluI AGCT 8 cut(s) 332, 340, 427, 625, 655, 790, 910, 957
Alw21I GWGCWC 5 cut(s) 137, 334, 429, 444, 627
Alw26I GTCTC 2 cut(s) 212, 885
Alw44I GTGCAC 1 cut(s) 133
AlwI GGATC 2 cut(s) 259, 267
Aor51HI AGCGCT 1 cut(s) 763
AoxI GGCC 1 cut(s) 922
ApaLI GTGCAC 1 cut(s) 133
ApeKI GCWGC 3 cut(s) 337, 638, 907
ApoI RAATTY 1 cut(s) 869
Asp718I GGTACC 1 cut(s) 551
AspLEI GCGC 4 cut(s) 67, 163, 684, 764
AspS9I GGNCC 4 cut(s) 70, 182, 383, 729
AsuC2I CCSGG 1 cut(s) 594
AsuHPI GGTGA 4 cut(s) 9, 265, 434, 470
AsuNHI GCTAGC 1 cut(s) 758
AvaII GGWCC 4 cut(s) 70, 182, 383, 729
BaeGI GKGCMC 1 cut(s) 137
BanI GGYRCC 2 cut(s) 58, 551
BanII GRGCYC 3 cut(s) 334, 429, 627
BauI CACGAG 1 cut(s) 913
BbsI GAAGAC 1 cut(s) 662
Bbv12I GWGCWC 5 cut(s) 137, 334, 429, 444, 627
BbvI GCAGC 3 cut(s) 349, 625, 919
BceAI ACGGC 2 cut(s) 93, 115
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 2 cut(s) 420, 926
BcnI CCSGG 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 212, 885
BfaI CTAG 1 cut(s) 759
BfoI RGCGCY 3 cut(s) 68, 685, 765
BfuAI ACCTGC 1 cut(s) 461
BglII AGATCT 1 cut(s) 346
BisI GCNGC 6 cut(s) 90, 142, 145, 338, 639, 908
BlpI GCTNAGC 1 cut(s) 333
BlsI GCNGC 6 cut(s) 91, 143, 146, 339, 640, 909
Bme1390I CCNGG 3 cut(s) 420, 594, 926
Bme18I GGWCC 4 cut(s) 70, 182, 383, 729
BmeRI GACNNNNNGTC 1 cut(s) 188
BmgT120I GGNCC 4 cut(s) 70, 182, 383, 729
BmiI GGNNCC 4 cut(s) 60, 184, 553, 730
BmrFI CCNGG 3 cut(s) 420, 594, 926
BmrI ACTGGG 1 cut(s) 180
BmtI GCTAGC 1 cut(s) 762
BmuI ACTGGG 1 cut(s) 180
BpiI GAAGAC 1 cut(s) 662
BplI GAGNNNNNCTC 2 cut(s) 609, 641
BpmI CTGGAG 1 cut(s) 947
Bpu1102I GCTNAGC 1 cut(s) 333
BpuEI CTTGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 594
BsaHI GRCGYC 1 cut(s) 80
BsaJI CCNNGG 4 cut(s) 418, 435, 464, 664
Bsc4I CCNNNNNNNGG 7 cut(s) 191, 419, 436, 470, 665, 839, 840
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 1 cut(s) 186
BseBI CCWGG 2 cut(s) 420, 926
BseDI CCNNGG 4 cut(s) 418, 435, 464, 664
BseGI GGATG 1 cut(s) 247
BseLI CCNNNNNNNGG 7 cut(s) 191, 419, 436, 470, 665, 839, 840
BseMII CTCAG 2 cut(s) 347, 515
BseNI ACTGG 1 cut(s) 186
BseRI GAGGAG 4 cut(s) 225, 228, 452, 903
BseSI GKGCMC 1 cut(s) 137
BseXI GCAGC 3 cut(s) 349, 625, 919
BsgI GTGCAG 1 cut(s) 659
Bsh1236I CGCG 2 cut(s) 163, 880
BshFI GGCC 1 cut(s) 924
BshNI GGYRCC 2 cut(s) 58, 551
BsiHKAI GWGCWC 5 cut(s) 137, 334, 429, 444, 627
BsiSI CCGG 2 cut(s) 62, 594
BslI CCNNNNNNNGG 7 cut(s) 191, 419, 436, 470, 665, 839, 840
BsmAI GTCTC 2 cut(s) 212, 885
BsmBI CGTCTC 2 cut(s) 212, 885
BsnI GGCC 1 cut(s) 924
Bsp1286I GDGCHC 5 cut(s) 137, 334, 429, 444, 627
Bsp1407I TGTACA 1 cut(s) 899
Bsp143I GATC 5 cut(s) 259, 264, 346, 540, 750
Bsp1720I GCTNAGC 1 cut(s) 333
BspACI CCGC 8 cut(s) 90, 141, 144, 147, 200, 207, 880, 997
BspANI GGCC 1 cut(s) 924
BspCNI CTCAG 2 cut(s) 346, 514
BspFNI CGCG 2 cut(s) 163, 880
BspLI GGNNCC 4 cut(s) 60, 184, 553, 730
BspMI ACCTGC 1 cut(s) 461
BspOI GCTAGC 1 cut(s) 762
BspPI GGATC 2 cut(s) 259, 267
BspT107I GGYRCC 2 cut(s) 58, 551
BsrFI RCCGGY 1 cut(s) 61
BsrGI TGTACA 1 cut(s) 899
BsrI ACTGG 1 cut(s) 186
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 4 cut(s) 418, 435, 464, 664
BssMI GATC 5 cut(s) 259, 264, 346, 540, 750
BssNI GRCGYC 1 cut(s) 80
BssSI CACGAG 1 cut(s) 913
BssT1I CCWWGG 2 cut(s) 464, 664
Bst2BI CACGAG 1 cut(s) 913
Bst2UI CCWGG 2 cut(s) 420, 926
Bst4CI ACNGT 4 cut(s) 21, 58, 449, 458
BstACI GRCGYC 1 cut(s) 80
BstAUI TGTACA 1 cut(s) 899
BstC8I GCNNGC 3 cut(s) 63, 760, 959
BstDEI CTNAG 3 cut(s) 333, 501, 1008
BstENI CCTNNNNNAGG 3 cut(s) 434, 468, 663
BstF5I GGATG 1 cut(s) 247
BstFNI CGCG 2 cut(s) 163, 880
BstH2I RGCGCY 3 cut(s) 68, 685, 765
BstHHI GCGC 4 cut(s) 67, 163, 684, 764
BstKTI GATC 5 cut(s) 262, 267, 349, 543, 753
BstMAI GTCTC 2 cut(s) 212, 885
BstMBI GATC 5 cut(s) 259, 264, 346, 540, 750
BstMWI GCNNNNNNNGC 4 cut(s) 86, 141, 158, 787
BstNI CCWGG 2 cut(s) 420, 926
BstSCI CCNGG 3 cut(s) 418, 592, 924
BstSLI GKGCMC 1 cut(s) 137
BstUI CGCG 2 cut(s) 163, 880
BstV1I GCAGC 3 cut(s) 349, 625, 919
BstV2I GAAGAC 1 cut(s) 662
BstX2I RGATCY 2 cut(s) 264, 346
BstYI RGATCY 2 cut(s) 264, 346
BsuRI GGCC 1 cut(s) 924
BtsCI GGATG 1 cut(s) 247
BtsI GCAGTG 1 cut(s) 604
BtsIMutI CAGTG 2 cut(s) 193, 604
BveI ACCTGC 1 cut(s) 461
Cac8I GCNNGC 3 cut(s) 63, 760, 959
CfoI GCGC 4 cut(s) 67, 163, 684, 764
Cfr10I RCCGGY 1 cut(s) 61
Cfr13I GGNCC 4 cut(s) 70, 182, 383, 729
CseI GACGC 1 cut(s) 69
Csp6I GTAC 2 cut(s) 552, 900
CviAII CATG 3 cut(s) 414, 709, 950
CviQI GTAC 2 cut(s) 552, 900
DdeI CTNAG 3 cut(s) 333, 501, 1008
DpnI GATC 5 cut(s) 261, 266, 348, 542, 752
DpnII GATC 5 cut(s) 259, 264, 346, 540, 750
DrdI GACNNNNNNGTC 2 cut(s) 226, 530
DriI GACNNNNNGTC 1 cut(s) 188
DseDI GACNNNNNNGTC 2 cut(s) 226, 530
Eam1105I GACNNNNNGTC 1 cut(s) 188
EciI GGCGGA 2 cut(s) 215, 222
Ecl136II GAGCTC 3 cut(s) 332, 427, 625
Eco130I CCWWGG 2 cut(s) 464, 664
Eco147I AGGCCT 1 cut(s) 924
Eco24I GRGCYC 3 cut(s) 334, 429, 627
Eco47I GGWCC 4 cut(s) 70, 182, 383, 729
Eco47III AGCGCT 1 cut(s) 763
Eco53kI GAGCTC 3 cut(s) 332, 427, 625
Eco57I CTGAAG 2 cut(s) 536, 628
EcoICRI GAGCTC 3 cut(s) 332, 427, 625
EcoNI CCTNNNNNAGG 3 cut(s) 434, 468, 663
EcoO109I RGGNCCY 1 cut(s) 383
EcoRII CCWGG 2 cut(s) 418, 924
EcoT14I CCWWGG 2 cut(s) 464, 664
EcoT38I GRGCYC 3 cut(s) 334, 429, 627
ErhI CCWWGG 2 cut(s) 464, 664
Esp3I CGTCTC 2 cut(s) 212, 885
FaeI CATG 3 cut(s) 417, 712, 953
FatI CATG 3 cut(s) 413, 708, 949
FauNDI CATATG 2 cut(s) 285, 777
Fnu4HI GCNGC 6 cut(s) 90, 142, 145, 338, 639, 908
FokI GGATG 1 cut(s) 254
FriOI GRGCYC 3 cut(s) 334, 429, 627
Fsp4HI GCNGC 6 cut(s) 90, 142, 145, 338, 639, 908
FspBI CTAG 1 cut(s) 759
GlaI GCGC 4 cut(s) 66, 162, 683, 763
GluI GCNGC 6 cut(s) 90, 142, 145, 338, 639, 908
GsuI CTGGAG 1 cut(s) 947
HaeII RGCGCY 3 cut(s) 68, 685, 765
HaeIII GGCC 1 cut(s) 924
HapII CCGG 2 cut(s) 62, 594
HgaI GACGC 1 cut(s) 69
HhaI GCGC 4 cut(s) 67, 163, 684, 764
Hin1I GRCGYC 1 cut(s) 80
Hin1II CATG 3 cut(s) 417, 712, 953
Hin6I GCGC 4 cut(s) 65, 161, 682, 762
HinP1I GCGC 4 cut(s) 65, 161, 682, 762
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 788
HinfI GANTC 3 cut(s) 389, 524, 846
HpaII CCGG 2 cut(s) 62, 594
HphI GGTGA 4 cut(s) 9, 265, 434, 470
Hpy166II GTNNAC 4 cut(s) 24, 135, 182, 900
Hpy188I TCNGA 8 cut(s) 264, 269, 394, 516, 560, 647, 733, 938
Hpy188III TCNNGA 1 cut(s) 913
Hpy8I GTNNAC 4 cut(s) 24, 135, 182, 900
Hpy99I CGWCG 5 cut(s) 31, 34, 37, 130, 235
HpyAV CCTTC 7 cut(s) 34, 229, 250, 301, 374, 652, 669
HpyCH4III ACNGT 4 cut(s) 21, 58, 449, 458
HpyCH4IV ACGT 2 cut(s) 26, 297
HpyCH4V TGCA 6 cut(s) 135, 283, 289, 355, 571, 676
HpyF10VI GCNNNNNNNGC 4 cut(s) 86, 141, 158, 787
HpyF3I CTNAG 3 cut(s) 333, 501, 1008
HpySE526I ACGT 2 cut(s) 26, 297
Hsp92I GRCGYC 1 cut(s) 80
Hsp92II CATG 3 cut(s) 417, 712, 953
HspAI GCGC 4 cut(s) 65, 161, 682, 762
KpnI GGTACC 1 cut(s) 555
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 5 cut(s) 259, 264, 346, 540, 750
LmnI GCTCC 2 cut(s) 432, 439
Lsp1109I GCAGC 3 cut(s) 349, 625, 919
MaeI CTAG 1 cut(s) 759
MaeII ACGT 2 cut(s) 26, 297
MaeIII GTNAC 2 cut(s) 15, 449
MalI GATC 5 cut(s) 261, 266, 348, 542, 752
MboI GATC 5 cut(s) 259, 264, 346, 540, 750
MboII GAAGA 6 cut(s) 91, 94, 356, 662, 764, 841
MflI RGATCY 2 cut(s) 264, 346
MhlI GDGCHC 5 cut(s) 137, 334, 429, 444, 627
MluCI AATT 3 cut(s) 312, 356, 869
MlyI GAGTC 1 cut(s) 518
MmeI TCCRAC 2 cut(s) 781, 916
MroNI GCCGGC 1 cut(s) 61
MseI TTAA 3 cut(s) 315, 588, 966
MslI CAYNNNNRTG 1 cut(s) 948
MspI CCGG 2 cut(s) 62, 594
MspR9I CCNGG 3 cut(s) 420, 594, 926
MvaI CCWGG 2 cut(s) 420, 926
MvnI CGCG 2 cut(s) 163, 880
MwoI GCNNNNNNNGC 4 cut(s) 86, 141, 158, 787
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 1 cut(s) 594
NdeI CATATG 2 cut(s) 285, 777
NdeII GATC 5 cut(s) 259, 264, 346, 540, 750
NgoMIV GCCGGC 1 cut(s) 61
NheI GCTAGC 1 cut(s) 758
NlaIII CATG 3 cut(s) 417, 712, 953
NlaIV GGNNCC 4 cut(s) 60, 184, 553, 730
NmuCI GTSAC 1 cut(s) 15
PaqCI CACCTGC 1 cut(s) 461
PceI AGGCCT 1 cut(s) 924
PcsI WCGNNNNNNNCGW 2 cut(s) 35, 227
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 389, 846
PflMI CCANNNNNTGG 1 cut(s) 419
PfoI TCCNGGA 1 cut(s) 592
PkrI GCNGC 6 cut(s) 91, 143, 146, 339, 640, 909
PleI GAGTC 1 cut(s) 518
PpsI GAGTC 1 cut(s) 518
PpuMI RGGWCCY 1 cut(s) 383
Psp124BI GAGCTC 3 cut(s) 334, 429, 627
Psp5II RGGWCCY 1 cut(s) 383
Psp6I CCWGG 2 cut(s) 418, 924
PspGI CCWGG 2 cut(s) 418, 924
PspN4I GGNNCC 4 cut(s) 60, 184, 553, 730
PspPI GGNCC 4 cut(s) 70, 182, 383, 729
PspPPI RGGWCCY 1 cut(s) 383
PsuI RGATCY 2 cut(s) 264, 346
RsaI GTAC 2 cut(s) 553, 901
RsaNI GTAC 2 cut(s) 552, 900
RseI CAYNNNNRTG 1 cut(s) 948
SacI GAGCTC 3 cut(s) 334, 429, 627
SaqAI TTAA 3 cut(s) 315, 588, 966
SatI GCNGC 6 cut(s) 90, 142, 145, 338, 639, 908
Sau3AI GATC 5 cut(s) 259, 264, 346, 540, 750
Sau96I GGNCC 4 cut(s) 70, 182, 383, 729
SchI GAGTC 1 cut(s) 518
ScrFI CCNGG 3 cut(s) 420, 594, 926
SduI GDGCHC 5 cut(s) 137, 334, 429, 444, 627
SinI GGWCC 4 cut(s) 70, 182, 383, 729
SmiMI CAYNNNNRTG 1 cut(s) 948
SmlI CTYRAG 1 cut(s) 536
SmoI CTYRAG 1 cut(s) 536
Sse9I AATT 3 cut(s) 312, 356, 869
SseBI AGGCCT 1 cut(s) 924
SsiI CCGC 8 cut(s) 90, 141, 144, 147, 200, 207, 880, 997
SspMI CTAG 1 cut(s) 759
SstI GAGCTC 3 cut(s) 334, 429, 627
StuI AGGCCT 1 cut(s) 924
StyD4I CCNGG 3 cut(s) 418, 592, 924
StyI CCWWGG 2 cut(s) 464, 664
TaaI ACNGT 4 cut(s) 21, 58, 449, 458
TaiI ACGT 2 cut(s) 29, 300
TaqI TCGA 2 cut(s) 233, 987
TasI AATT 3 cut(s) 312, 356, 869
TatI WGTACW 1 cut(s) 899
TauI GCSGC 3 cut(s) 92, 144, 147
TfiI GAWTC 2 cut(s) 389, 846
Tru1I TTAA 3 cut(s) 315, 588, 966
Tru9I TTAA 3 cut(s) 315, 588, 966
TscAI CASTG 2 cut(s) 193, 611
TseFI GTSAC 1 cut(s) 15
TseI GCWGC 3 cut(s) 337, 638, 907
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 3 cut(s) 393, 565, 764
TspRI CASTG 2 cut(s) 193, 611
Van91I CCANNNNNTGG 1 cut(s) 419
VneI GTGCAC 1 cut(s) 133
VpaK11BI GGWCC 4 cut(s) 70, 182, 383, 729
XagI CCTNNNNNAGG 3 cut(s) 434, 468, 663
XapI RAATTY 1 cut(s) 869
XcmI CCANNNNNNNNNTGG 1 cut(s) 193
XspI CTAG 1 cut(s) 759
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.