Rw3G003350

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0013395
Physical Location & Seq
Forward (+)
2871138 .. 2873353
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G003350.1

Sequence Viewer

Length: 753 bp
ATGGCTCTCTCCTCGTCTTCGTCGTCACCGTCCAAGGGCATAGTGGTAACTGTCCCGGTGCTGGTCCTCTCGGCCTCGGTCGCCGCCGTCTTCTTCTTCTTCCTCCTCTCCTCTCTCTCGTCCTGCTCCTGCGGCGGCGGCCGTATCGCCGTCGCCGGCGTTCCGAGTCAGTCCGGCGGAGGAGATGTGGCTGCGGCGACCGGAAGGTGGGAGAGCGGCGAGACGATACGGTCGTCAAAGGAGGATGTGGAGTGGGTGAAGGATCAGATCCGAGTGAATGGGCTGCATATGCAGGATAACGTGTTGCGTAAGGGAATTAACCCTAGGACTCGGGCTCAGCAGCTCCAGGATCTCTTGCAGTACAAGGGTATATCTCACTATGAAGGACCTGACTCTGGAAATCACACTGCCCTCCCATGCCCTGGTGAGCTCCTTACTGAAGAACACCACAGTAACTATGGTGAGCCTTGGGCAGGCGGGCGAGATGTTTTTGAGTTCCTTGCTCAGTCCAGCCATCTTAAGCCTGATTCAAAGGTTCTTGAGATTGGCTGTGGTACCCTCCGAGTTGGTTTGCACTTCATTCGTTATCTTCATCCCGGACACTTCCACTGTCTCGAAAGAGATGAGCTCTCACTAATGGCTGCCTTTAGATATGAGCTTCCTTCCCAAGGTCTTCTTCATAAGCGCCCTTTAATTGTGAAAGGTGACAACATGGACTTCTCAGATTTGGTTCTGACGTTGTGTATGATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_23

Protein Analysis

250

Amino Acids

27.17

Weight (kDa)

6.25

Isoelectric Point (pI)

46.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05100
fragaria_vesca FvH4_6g03940
malus_domestica MD04G1217100.v1.1 MD12G1234100.v1.1
prunus_persica Prupe.6G337200_v2.0.a1 Prupe.6G337200_v2.0.a1
pyrus_communis pycom04g19320 pycom12g21650 pycom16g23200
rosa_chinensis RchiOBHm_Chr3g0452511
rosa_laevigata RLG00000025596
rosa_roxburghii Rroxscaffold_6G00429540
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043200 Rh3BG044900 Rh3CG043500 Rh3DG044300
rosa_wichuraiana Rw3G003350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
Acc65I GGTACC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 554
AccB7I CCANNNNNTGG 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 216
AciI CCGC 8 cut(s) 84, 132, 135, 138, 177, 194, 216, 477
AclWI GGATC 3 cut(s) 262, 270, 357
AcoI YGGCCR 1 cut(s) 139
AcuI CTGAAG 1 cut(s) 459
AfaI GTAC 2 cut(s) 362, 556
AfiI CCNNNNNNNGG 7 cut(s) 35, 61, 207, 395, 422, 473, 668
AflII CTTAAG 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 1 cut(s) 531
AjnI CCWGG 2 cut(s) 345, 421
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 4 cut(s) 343, 430, 628, 658
AluI AGCT 4 cut(s) 343, 430, 628, 658
Alw21I GWGCWC 2 cut(s) 432, 630
Alw26I GTCTC 2 cut(s) 215, 617
AlwI GGATC 3 cut(s) 262, 270, 357
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 2 cut(s) 72, 139
ApeKI GCWGC 4 cut(s) 191, 283, 340, 641
Asp718I GGTACC 1 cut(s) 554
AspA2I CCTAGG 1 cut(s) 323
AspLEI GCGC 1 cut(s) 687
AspS9I GGNCC 2 cut(s) 64, 386
AsuC2I CCSGG 2 cut(s) 56, 597
AsuHPI GGTGA 5 cut(s) 18, 268, 437, 473, 716
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 64, 386
AvrII CCTAGG 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 218, 251
BanI GGYRCC 1 cut(s) 554
BanII GRGCYC 3 cut(s) 337, 432, 630
BbsI GAAGAC 3 cut(s) 9, 82, 665
Bbv12I GWGCWC 2 cut(s) 432, 630
BbvI GCAGC 4 cut(s) 178, 270, 352, 628
BccI CCATC 1 cut(s) 522
BceAI ACGGC 3 cut(s) 71, 126, 134
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 2 cut(s) 347, 423
BcnI CCSGG 2 cut(s) 56, 597
BcoDI GTCTC 2 cut(s) 215, 617
BfaI CTAG 1 cut(s) 324
BfoI RGCGCY 1 cut(s) 688
BfrI CTTAAG 1 cut(s) 518
BlnI CCTAGG 1 cut(s) 323
BlpI GCTNAGC 1 cut(s) 336
Bme1390I CCNGG 4 cut(s) 56, 347, 423, 597
Bme18I GGWCC 2 cut(s) 64, 386
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 2 cut(s) 64, 386
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 4 cut(s) 56, 347, 423, 597
BpiI GAAGAC 3 cut(s) 9, 82, 665
BplI GAGNNNNNCTC 2 cut(s) 612, 644
BpmI CTGGAG 1 cut(s) 329
Bpu1102I GCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 2 cut(s) 56, 597
BsaJI CCNNGG 6 cut(s) 33, 75, 323, 421, 467, 667
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 7 cut(s) 35, 61, 207, 395, 422, 473, 668
Bse118I RCCGGY 1 cut(s) 155
BseBI CCWGG 2 cut(s) 347, 423
BseDI CCNNGG 6 cut(s) 33, 75, 323, 421, 467, 667
BseGI GGATG 2 cut(s) 250, 592
BseLI CCNNNNNNNGG 7 cut(s) 35, 61, 207, 395, 422, 473, 668
BseMII CTCAG 3 cut(s) 350, 518, 735
BseRI GAGGAG 3 cut(s) 95, 100, 195
BseX3I CGGCCG 1 cut(s) 139
BseXI GCAGC 4 cut(s) 178, 270, 352, 628
Bsh1285I CGRYCG 4 cut(s) 81, 142, 201, 233
BshFI GGCC 2 cut(s) 74, 141
BshNI GGYRCC 1 cut(s) 554
BsiEI CGRYCG 4 cut(s) 81, 142, 201, 233
BsiHKAI GWGCWC 2 cut(s) 432, 630
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 5 cut(s) 56, 156, 174, 201, 597
BslFI GGGAC 1 cut(s) 38
BslI CCNNNNNNNGG 7 cut(s) 35, 61, 207, 395, 422, 473, 668
BsmAI GTCTC 2 cut(s) 215, 617
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 1 cut(s) 38
BsnI GGCC 2 cut(s) 74, 141
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 3 cut(s) 337, 432, 630
Bsp143I GATC 3 cut(s) 262, 267, 349
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 8 cut(s) 84, 132, 135, 138, 177, 194, 216, 477
BspANI GGCC 2 cut(s) 74, 141
BspCNI CTCAG 3 cut(s) 349, 517, 734
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 3 cut(s) 262, 270, 357
BspT107I GGYRCC 1 cut(s) 554
BspTI CTTAAG 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 216
BsrFI RCCGGY 1 cut(s) 155
BssAI RCCGGY 1 cut(s) 155
BssECI CCNNGG 6 cut(s) 33, 75, 323, 421, 467, 667
BssMI GATC 3 cut(s) 262, 267, 349
BssT1I CCWWGG 4 cut(s) 33, 323, 467, 667
Bst2UI CCWGG 2 cut(s) 347, 423
Bst4CI ACNGT 5 cut(s) 30, 52, 231, 452, 611
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 3 cut(s) 157, 475, 479
BstDEI CTNAG 3 cut(s) 336, 504, 721
BstENI CCTNNNNNAGG 2 cut(s) 471, 666
BstF5I GGATG 2 cut(s) 250, 592
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 1 cut(s) 687
BstKTI GATC 3 cut(s) 265, 270, 352
BstMAI GTCTC 2 cut(s) 215, 617
BstMBI GATC 3 cut(s) 262, 267, 349
BstMCI CGRYCG 4 cut(s) 81, 142, 201, 233
BstMWI GCNNNNNNNGC 4 cut(s) 80, 132, 138, 289
BstNI CCWGG 2 cut(s) 347, 423
BstSCI CCNGG 4 cut(s) 54, 345, 421, 595
BstV1I GCAGC 4 cut(s) 178, 270, 352, 628
BstV2I GAAGAC 3 cut(s) 9, 82, 665
BstX2I RGATCY 2 cut(s) 267, 349
BstYI RGATCY 2 cut(s) 267, 349
BstZI CGGCCG 1 cut(s) 139
BsuRI GGCC 2 cut(s) 74, 141
BtsCI GGATG 2 cut(s) 250, 592
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 405, 607
Cac8I GCNNGC 3 cut(s) 157, 475, 479
CfoI GCGC 1 cut(s) 687
Cfr10I RCCGGY 1 cut(s) 155
Cfr13I GGNCC 2 cut(s) 64, 386
Csp6I GTAC 2 cut(s) 361, 555
CviAII CATG 2 cut(s) 417, 712
CviQI GTAC 2 cut(s) 361, 555
DdeI CTNAG 3 cut(s) 336, 504, 721
DpnI GATC 3 cut(s) 264, 269, 351
DpnII GATC 3 cut(s) 262, 267, 349
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
EaeI YGGCCR 1 cut(s) 139
EagI CGGCCG 1 cut(s) 139
EciI GGCGGA 1 cut(s) 192
Ecl136II GAGCTC 2 cut(s) 430, 628
EclXI CGGCCG 1 cut(s) 139
Eco130I CCWWGG 4 cut(s) 33, 323, 467, 667
Eco24I GRGCYC 3 cut(s) 337, 432, 630
Eco47I GGWCC 2 cut(s) 64, 386
Eco52I CGGCCG 1 cut(s) 139
Eco53kI GAGCTC 2 cut(s) 430, 628
Eco57I CTGAAG 1 cut(s) 459
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 2 cut(s) 430, 628
EcoNI CCTNNNNNAGG 2 cut(s) 471, 666
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 2 cut(s) 345, 421
EcoT14I CCWWGG 4 cut(s) 33, 323, 467, 667
EcoT38I GRGCYC 3 cut(s) 337, 432, 630
ErhI CCWWGG 4 cut(s) 33, 323, 467, 667
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 2 cut(s) 420, 715
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 1 cut(s) 38
FatI CATG 2 cut(s) 416, 711
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 1 cut(s) 288
FokI GGATG 2 cut(s) 257, 579
FriOI GRGCYC 3 cut(s) 337, 432, 630
FspBI CTAG 1 cut(s) 324
GlaI GCGC 1 cut(s) 686
GsuI CTGGAG 1 cut(s) 329
HaeII RGCGCY 1 cut(s) 688
HaeIII GGCC 2 cut(s) 74, 141
HapII CCGG 5 cut(s) 56, 156, 174, 201, 597
HhaI GCGC 1 cut(s) 687
Hin1II CATG 2 cut(s) 420, 715
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HinfI GANTC 4 cut(s) 166, 328, 392, 527
HpaII CCGG 5 cut(s) 56, 156, 174, 201, 597
HphI GGTGA 5 cut(s) 18, 268, 437, 473, 716
Hpy188I TCNGA 6 cut(s) 165, 267, 272, 563, 724, 735
Hpy188III TCNNGA 3 cut(s) 396, 539, 614
Hpy99I CGWCG 2 cut(s) 25, 155
HpyAV CCTTC 4 cut(s) 198, 253, 377, 672
HpyCH4III ACNGT 5 cut(s) 30, 52, 231, 452, 611
HpyCH4IV ACGT 2 cut(s) 300, 737
HpyCH4V TGCA 4 cut(s) 286, 292, 358, 574
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 132, 138, 289
HpyF3I CTNAG 3 cut(s) 336, 504, 721
HpySE526I ACGT 2 cut(s) 300, 737
Hsp92II CATG 2 cut(s) 420, 715
HspAI GCGC 1 cut(s) 685
KpnI GGTACC 1 cut(s) 558
KroI GCCGGC 1 cut(s) 155
KroNI GCCGGC 1 cut(s) 157
Kzo9I GATC 3 cut(s) 262, 267, 349
LmnI GCTCC 3 cut(s) 131, 348, 435
Lsp1109I GCAGC 4 cut(s) 178, 270, 352, 628
MaeI CTAG 1 cut(s) 324
MaeII ACGT 2 cut(s) 300, 737
MaeIII GTNAC 4 cut(s) 24, 46, 452, 704
MalI GATC 3 cut(s) 264, 269, 351
MbiI CCGCTC 1 cut(s) 216
MboI GATC 3 cut(s) 262, 267, 349
MboII GAAGA 9 cut(s) 9, 82, 85, 88, 91, 452, 581, 665, 668
MflI RGATCY 2 cut(s) 267, 349
MhlI GDGCHC 3 cut(s) 337, 432, 630
MluCI AATT 2 cut(s) 315, 693
MlyI GAGTC 3 cut(s) 175, 322, 386
MreI CGCCGGCG 1 cut(s) 155
MroNI GCCGGC 1 cut(s) 155
MseI TTAA 3 cut(s) 318, 519, 692
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 5 cut(s) 56, 156, 174, 201, 597
MspR9I CCNGG 4 cut(s) 56, 347, 423, 597
MvaI CCWGG 2 cut(s) 347, 423
MwoI GCNNNNNNNGC 4 cut(s) 80, 132, 138, 289
NaeI GCCGGC 1 cut(s) 157
NciI CCSGG 2 cut(s) 56, 597
NdeI CATATG 1 cut(s) 288
NdeII GATC 3 cut(s) 262, 267, 349
NgoMIV GCCGGC 1 cut(s) 155
NlaIII CATG 2 cut(s) 420, 715
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 1 cut(s) 50
NmuCI GTSAC 2 cut(s) 24, 704
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 26, 230
PdiI GCCGGC 1 cut(s) 157
PfeI GAWTC 1 cut(s) 527
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 2 cut(s) 345, 595
PleI GAGTC 3 cut(s) 174, 322, 386
PpsI GAGTC 3 cut(s) 174, 322, 386
PpuMI RGGWCCY 1 cut(s) 386
Psp124BI GAGCTC 2 cut(s) 432, 630
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 2 cut(s) 345, 421
PspGI CCWGG 2 cut(s) 345, 421
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 2 cut(s) 64, 386
PspPPI RGGWCCY 1 cut(s) 386
PsuI RGATCY 2 cut(s) 267, 349
RsaI GTAC 2 cut(s) 362, 556
RsaNI GTAC 2 cut(s) 361, 555
SacI GAGCTC 2 cut(s) 432, 630
SaqAI TTAA 3 cut(s) 318, 519, 692
Sau3AI GATC 3 cut(s) 262, 267, 349
Sau96I GGNCC 2 cut(s) 64, 386
SchI GAGTC 3 cut(s) 175, 322, 386
ScrFI CCNGG 4 cut(s) 56, 347, 423, 597
SduI GDGCHC 3 cut(s) 337, 432, 630
SgrAI CRCCGGYG 1 cut(s) 155
SinI GGWCC 2 cut(s) 64, 386
SmlI CTYRAG 2 cut(s) 518, 539
SmoI CTYRAG 2 cut(s) 518, 539
Sse9I AATT 2 cut(s) 315, 693
SsiI CCGC 8 cut(s) 84, 132, 135, 138, 177, 194, 216, 477
SspMI CTAG 1 cut(s) 324
SstI GAGCTC 2 cut(s) 432, 630
StyD4I CCNGG 4 cut(s) 54, 345, 421, 595
StyI CCWWGG 4 cut(s) 33, 323, 467, 667
TaaI ACNGT 5 cut(s) 30, 52, 231, 452, 611
TaiI ACGT 2 cut(s) 303, 740
TaqI TCGA 1 cut(s) 615
TaqII GACCGA 1 cut(s) 67
TasI AATT 2 cut(s) 315, 693
TatI WGTACW 1 cut(s) 360
TauI GCSGC 6 cut(s) 86, 135, 138, 141, 197, 219
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 3 cut(s) 318, 519, 692
Tru9I TTAA 3 cut(s) 318, 519, 692
TscAI CASTG 2 cut(s) 412, 614
TseFI GTSAC 2 cut(s) 24, 704
TseI GCWGC 4 cut(s) 191, 283, 340, 641
Tsp45I GTSAC 2 cut(s) 24, 704
TspDTI ATGAA 4 cut(s) 396, 568, 581, 668
TspRI CASTG 2 cut(s) 412, 614
Van91I CCANNNNNTGG 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 64, 386
XagI CCTNNNNNAGG 2 cut(s) 471, 666
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 455
XmaJI CCTAGG 1 cut(s) 323
XspI CTAG 1 cut(s) 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.