pycom12g21650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0013395
Physical Location & Seq
Forward (+)
22784299 .. 22786017
1719 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g21650.1

Sequence Viewer

Length: 786 bp
ATGGTCGTCGAAGGAGGATGTGGAGTGGGTGAAGGATCGATCCGAGTGAATGGGTTGCATATGCAAGATAACGTGTTGAGGAAGGGAATTAACCCTCGCACCAGAGCTCAGCAGCTTCAAGATCTCTTGGAATTCAAGGGTATATCTCATTATGAAGGACCTGATTCGGAAAACCATACTGCTCTCCCATGCCCTGGTGAGCTCCTAACTGAGGAGCACCACAGTAACTACGGTGAGCCTTGGGCAGGCGGGCGAGATATTTTTGAGTTCCTCGCTCAGTCCTCTCATCTGAAGCCCGAATCAATTGTCCTTGAGATTGGCTGTGGTACCCTCCGAGTTGGTTTGCATTTCATTCGCTATCTTAATCCCGGACACTTCCACTGTCTTGAAAGAGATGAGCTCTCACTAATGGCTGCCTTTAGATACGAGCTTCCTTCCCAAGGTCTTCTGCACAAGCGCCCCTTGATTGTGAAAGGCGACAGCATGGACTTCAGCAGATTTGGTTCAGATGTTGTTTATGACTTGATCGATGCTATTGCTGTGTTTCTTCATATGCCTGATAAGCTTGTGTGGGTTGGATTGGAGAGATTGACAAATAAACTGAAACCCTATGACGGGCGAATCTTCGTGTCCCATAATATCAAATTCTGTTCGCGGTTGGGAGGAGACGAATGTACAAAGCGGCTTACAAATTTAGGCCTGGAGTATGTCAGAAAGCATACACATGACAGCTTGCTATTTAACCACTACGAAATCTGGTTCGAGTTTAGGAGGACGAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_23

Protein Analysis

262

Amino Acids

29.77

Weight (kDa)

6.43

Isoelectric Point (pI)

37.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05100
fragaria_vesca FvH4_6g03940
malus_domestica MD04G1217100.v1.1 MD12G1234100.v1.1
prunus_persica Prupe.6G337200_v2.0.a1 Prupe.6G337200_v2.0.a1
pyrus_communis pycom04g19320 pycom12g21650 pycom16g23200
rosa_chinensis RchiOBHm_Chr3g0452511
rosa_laevigata RLG00000025596
rosa_roxburghii Rroxscaffold_6G00429540
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043200 Rh3BG044900 Rh3CG043500 Rh3DG044300
rosa_wichuraiana Rw3G003350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 194
AccII CGCG 1 cut(s) 655
AciI CCGC 3 cut(s) 249, 655, 682
AclWI GGATC 2 cut(s) 34, 43
AcsI RAATTY 3 cut(s) 131, 644, 691
AcuI CTGAAG 2 cut(s) 311, 475
AfaI GTAC 2 cut(s) 328, 676
AfiI CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 3 cut(s) 119, 136, 389
AjnI CCWGG 2 cut(s) 193, 699
AluBI AGCT 7 cut(s) 107, 115, 202, 400, 430, 565, 732
AluI AGCT 7 cut(s) 107, 115, 202, 400, 430, 565, 732
Alw21I GWGCWC 4 cut(s) 109, 204, 219, 402
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 2 cut(s) 34, 43
AoxI GGCC 1 cut(s) 697
ApeKI GCWGC 2 cut(s) 112, 413
ApoI RAATTY 3 cut(s) 131, 644, 691
ArsI GACNNNNNNTTYG 2 cut(s) 291, 323
Asp718I GGTACC 1 cut(s) 326
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 158
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 3 cut(s) 41, 209, 245
AvaII GGWCC 1 cut(s) 158
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 3 cut(s) 109, 204, 402
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 4 cut(s) 109, 204, 219, 402
BbvI GCAGC 2 cut(s) 124, 400
BcgI CGANNNNNNTGC 4 cut(s) 335, 369, 518, 552
BciT130I CCWGG 2 cut(s) 195, 701
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 460
BglII AGATCT 1 cut(s) 121
BisI GCNGC 3 cut(s) 113, 414, 683
BlpI GCTNAGC 1 cut(s) 108
BlsI GCNGC 3 cut(s) 114, 415, 684
Bme1390I CCNGG 3 cut(s) 195, 369, 701
Bme18I GGWCC 1 cut(s) 158
BmgT120I GGNCC 1 cut(s) 158
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 3 cut(s) 195, 369, 701
BmsI GCATC 1 cut(s) 520
BpiI GAAGAC 1 cut(s) 437
BplI GAGNNNNNCTC 2 cut(s) 384, 416
BpmI CTGGAG 1 cut(s) 722
Bpu1102I GCTNAGC 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 369
Bsa29I ATCGAT 2 cut(s) 38, 528
BsaJI CCNNGG 3 cut(s) 193, 239, 439
Bsc4I CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
BseBI CCWGG 2 cut(s) 195, 701
BseCI ATCGAT 2 cut(s) 38, 528
BseDI CCNNGG 3 cut(s) 193, 239, 439
BseGI GGATG 1 cut(s) 23
BseLI CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
BseMII CTCAG 3 cut(s) 122, 201, 290
BseRI GAGGAG 2 cut(s) 227, 678
BseXI GCAGC 2 cut(s) 124, 400
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 1 cut(s) 655
BshFI GGCC 1 cut(s) 699
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 2 cut(s) 38, 528
BsiHKAI GWGCWC 4 cut(s) 109, 204, 219, 402
BsiSI CCGG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 616
BslI CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
BsmAI GTCTC 1 cut(s) 660
BsmBI CGTCTC 1 cut(s) 660
BsmFI GGGAC 1 cut(s) 616
BsnI GGCC 1 cut(s) 699
Bsp1286I GDGCHC 4 cut(s) 109, 204, 219, 402
Bsp1407I TGTACA 1 cut(s) 674
Bsp143I GATC 4 cut(s) 35, 39, 121, 525
Bsp1720I GCTNAGC 1 cut(s) 108
BspACI CCGC 3 cut(s) 249, 655, 682
BspANI GGCC 1 cut(s) 699
BspCNI CTCAG 3 cut(s) 121, 202, 289
BspDI ATCGAT 2 cut(s) 38, 528
BspFNI CGCG 1 cut(s) 655
BspLI GGNNCC 1 cut(s) 328
BspPI GGATC 2 cut(s) 34, 43
BspT107I GGYRCC 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 674
BssECI CCNNGG 3 cut(s) 193, 239, 439
BssMI GATC 4 cut(s) 35, 39, 121, 525
BssT1I CCWWGG 2 cut(s) 239, 439
Bst2UI CCWGG 2 cut(s) 195, 701
Bst4CI ACNGT 3 cut(s) 224, 233, 383
BstAUI TGTACA 1 cut(s) 674
BstC8I GCNNGC 3 cut(s) 247, 251, 734
BstDEI CTNAG 3 cut(s) 108, 210, 276
BstENI CCTNNNNNAGG 3 cut(s) 209, 243, 438
BstF5I GGATG 1 cut(s) 23
BstFNI CGCG 1 cut(s) 655
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 4 cut(s) 38, 42, 124, 528
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 4 cut(s) 35, 39, 121, 525
BstMWI GCNNNNNNNGC 1 cut(s) 562
BstNI CCWGG 2 cut(s) 195, 701
BstSCI CCNGG 3 cut(s) 193, 367, 699
BstUI CGCG 1 cut(s) 655
BstV1I GCAGC 2 cut(s) 124, 400
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
Bsu15I ATCGAT 2 cut(s) 38, 528
BsuRI GGCC 1 cut(s) 699
BsuTUI ATCGAT 2 cut(s) 38, 528
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 3 cut(s) 247, 251, 734
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 1 cut(s) 158
ClaI ATCGAT 2 cut(s) 38, 528
Csp6I GTAC 2 cut(s) 327, 675
CviAII CATG 3 cut(s) 189, 484, 725
CviQI GTAC 2 cut(s) 327, 675
DdeI CTNAG 3 cut(s) 108, 210, 276
DpnI GATC 4 cut(s) 37, 41, 123, 527
DpnII GATC 4 cut(s) 35, 39, 121, 525
Ecl136II GAGCTC 3 cut(s) 107, 202, 400
Eco130I CCWWGG 2 cut(s) 239, 439
Eco147I AGGCCT 1 cut(s) 699
Eco24I GRGCYC 3 cut(s) 109, 204, 402
Eco47I GGWCC 1 cut(s) 158
Eco53kI GAGCTC 3 cut(s) 107, 202, 400
Eco57I CTGAAG 2 cut(s) 311, 475
EcoICRI GAGCTC 3 cut(s) 107, 202, 400
EcoNI CCTNNNNNAGG 3 cut(s) 209, 243, 438
EcoO109I RGGNCCY 1 cut(s) 158
EcoRI GAATTC 1 cut(s) 131
EcoRII CCWGG 2 cut(s) 193, 699
EcoT14I CCWWGG 2 cut(s) 239, 439
EcoT38I GRGCYC 3 cut(s) 109, 204, 402
ErhI CCWWGG 2 cut(s) 239, 439
Esp3I CGTCTC 1 cut(s) 660
FaeI CATG 3 cut(s) 192, 487, 728
FalI AAGNNNNNCTT 2 cut(s) 446, 478
FaqI GGGAC 1 cut(s) 616
FatI CATG 3 cut(s) 188, 483, 724
FauI CCCGC 1 cut(s) 242
FauNDI CATATG 2 cut(s) 60, 552
Fnu4HI GCNGC 3 cut(s) 113, 414, 683
FokI GGATG 1 cut(s) 30
FriOI GRGCYC 3 cut(s) 109, 204, 402
Fsp4HI GCNGC 3 cut(s) 113, 414, 683
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 113, 414, 683
GsuI CTGGAG 1 cut(s) 722
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 1 cut(s) 699
HapII CCGG 1 cut(s) 369
HhaI GCGC 1 cut(s) 459
Hin1II CATG 3 cut(s) 192, 487, 728
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 3 cut(s) 164, 299, 621
HpaII CCGG 1 cut(s) 369
HphI GGTGA 3 cut(s) 41, 209, 245
Hpy188I TCNGA 6 cut(s) 44, 169, 291, 335, 508, 713
Hpy188III TCNNGA 2 cut(s) 119, 386
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 6 cut(s) 5, 26, 76, 149, 444, 772
HpyCH4III ACNGT 3 cut(s) 224, 233, 383
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 58, 64, 346, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 562
HpyF3I CTNAG 3 cut(s) 108, 210, 276
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 3 cut(s) 192, 487, 728
HspAI GCGC 1 cut(s) 457
KpnI GGTACC 1 cut(s) 330
Kzo9I GATC 4 cut(s) 35, 39, 121, 525
LmnI GCTCC 2 cut(s) 207, 214
Lsp1109I GCAGC 2 cut(s) 124, 400
LweI GCATC 1 cut(s) 520
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 224
MalI GATC 4 cut(s) 37, 41, 123, 527
MboI GATC 4 cut(s) 35, 39, 121, 525
MboII GAAGA 3 cut(s) 437, 539, 616
MfeI CAATTG 1 cut(s) 303
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 4 cut(s) 109, 204, 219, 402
MluCI AATT 5 cut(s) 87, 131, 303, 644, 691
MmeI TCCRAC 1 cut(s) 556
MnlI CCTC 9 cut(s) 8, 72, 105, 205, 281, 292, 341, 656, 765
MseI TTAA 4 cut(s) 90, 363, 741, 784
MslI CAYNNNNRTG 1 cut(s) 723
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 3 cut(s) 195, 369, 701
MunI CAATTG 1 cut(s) 303
MvaI CCWGG 2 cut(s) 195, 701
MvnI CGCG 1 cut(s) 655
MwoI GCNNNNNNNGC 1 cut(s) 562
NciI CCSGG 1 cut(s) 369
NdeI CATATG 2 cut(s) 60, 552
NdeII GATC 4 cut(s) 35, 39, 121, 525
NlaIII CATG 3 cut(s) 192, 487, 728
NlaIV GGNNCC 1 cut(s) 328
PceI AGGCCT 1 cut(s) 699
PfeI GAWTC 3 cut(s) 164, 299, 621
PflMI CCANNNNNTGG 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 3 cut(s) 114, 415, 684
PpuMI RGGWCCY 1 cut(s) 158
Psp124BI GAGCTC 3 cut(s) 109, 204, 402
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 193, 699
PspGI CCWGG 2 cut(s) 193, 699
PspN4I GGNNCC 1 cut(s) 328
PspPI GGNCC 1 cut(s) 158
PspPPI RGGWCCY 1 cut(s) 158
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 328, 676
RsaNI GTAC 2 cut(s) 327, 675
RseI CAYNNNNRTG 1 cut(s) 723
SacI GAGCTC 3 cut(s) 109, 204, 402
SaqAI TTAA 4 cut(s) 90, 363, 741, 784
SatI GCNGC 3 cut(s) 113, 414, 683
Sau3AI GATC 4 cut(s) 35, 39, 121, 525
Sau96I GGNCC 1 cut(s) 158
ScrFI CCNGG 3 cut(s) 195, 369, 701
SduI GDGCHC 4 cut(s) 109, 204, 219, 402
SfaNI GCATC 1 cut(s) 520
SinI GGWCC 1 cut(s) 158
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
Sse9I AATT 5 cut(s) 87, 131, 303, 644, 691
SseBI AGGCCT 1 cut(s) 699
SsiI CCGC 3 cut(s) 249, 655, 682
SstI GAGCTC 3 cut(s) 109, 204, 402
StuI AGGCCT 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 193, 367, 699
StyI CCWWGG 2 cut(s) 239, 439
TaaI ACNGT 3 cut(s) 224, 233, 383
TaiI ACGT 1 cut(s) 75
TaqI TCGA 4 cut(s) 9, 38, 528, 762
TasI AATT 5 cut(s) 87, 131, 303, 644, 691
TatI WGTACW 1 cut(s) 674
TauI GCSGC 1 cut(s) 685
TfiI GAWTC 3 cut(s) 164, 299, 621
Tru1I TTAA 4 cut(s) 90, 363, 741, 784
Tru9I TTAA 4 cut(s) 90, 363, 741, 784
TscAI CASTG 1 cut(s) 386
TseI GCWGC 2 cut(s) 112, 413
TspDTI ATGAA 3 cut(s) 168, 340, 539
TspRI CASTG 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 194
VpaK11BI GGWCC 1 cut(s) 158
XagI CCTNNNNNAGG 3 cut(s) 209, 243, 438
XapI RAATTY 3 cut(s) 131, 644, 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.