MD12G1234100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0013395
Physical Location & Seq
Forward (+)
30955939 .. 30958087
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1234100.v1.1.491

Sequence Viewer

Length: 1035 bp
ATGGCTCTGTGGTCGTCACCGTCAAATTCGTCGGCTAAGGGGATAGTGATAACGGTGCCGGCGCTGGTTCTGACGGCGTCAGTGGCCGCGGTTTTCTTCTTCTTTCTCTTGTACTCTCTCTCGTCGCCGTGCACTTGCGGCGGCCGCGACGCCGGTGGGGCTGCTAGTGCTAGTTTTGCGAGAGTCGGCGATGTCGATGGACCGAGTGTCGGCGTGGCGGAGGGCGGAGGAGGAGGAGGAGAGACGATATGGTCGTCGAAGGAGGATGTGGAGTGGGTGAAGGATCAGATCCGAGTGAATGGGTTGCATATGCAGGATAACGTGTTGAGGAAGGGAATTAACCCTCGCACCAGAGCTCAGCAGCTTCAAGATCTCTTGGAGTTCAAGGGTATATCTCATTATGAAGGACCTGATTCAGAAAAGCATACTGCTCTCCCATGCCCTGGTGAGCTCCTAACTGAGGAGCACCACAGTAACTACGGTGAGCCTTGGGCAGGCGGGCGAGATGTTTTTGAGTTCCTCGCTCAGTCCTCTCATCTGAAGCCCGACTCAATTGTCCTTGAGATTGGCTGTGGTACCCTCCGAGTCGGTTTGCATTTCATTCGCTATCTTAATCCTGGACACTTCCACTGTCTTGAAAGAGATGAGCTCTCACTAATGGCTGCCTTTAGATACGAGCTTCCTTCCCAAGGTCTTCTGCACAAGCGCCCTTTGATTGTGAAAGGCGACAACATGGACTTCGGCGGATTTGGTTCAGATGTTGTTTATGACTTGATCTATGCTAGTGCTGTGTTTCTTCATATGCCTGATAAGCTTGTGTGGGTTGGATTGGAGAGATTGACAAATAAACTGAAACCCTATGACGGGCGAATCTTCGTGTCCCATAATATCAAATTCTGTTCGCGGTTGGGAGGAGACGAATGTACAAAGCGGCTTACAAATTTAGGCCTGGAGTATGTCGGAAAGCATACACACGACAGCTTGCTATTCAACCACTACGAAATCTGGTTCGAGTTTAGGAGGACGAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_23

Protein Analysis

345

Amino Acids

38.01

Weight (kDa)

6.2

Isoelectric Point (pI)

38.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 187 - 289 2e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05100
fragaria_vesca FvH4_6g03940
malus_domestica MD04G1217100.v1.1 MD12G1234100.v1.1
prunus_persica Prupe.6G337200_v2.0.a1 Prupe.6G337200_v2.0.a1
pyrus_communis pycom04g19320 pycom12g21650 pycom16g23200
rosa_chinensis RchiOBHm_Chr3g0452511
rosa_laevigata RLG00000025596
rosa_roxburghii Rroxscaffold_6G00429540
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043200 Rh3BG044900 Rh3CG043500 Rh3DG044300
rosa_wichuraiana Rw3G003350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 250, 554
Acc65I GGTACC 1 cut(s) 575
AccB1I GGYRCC 2 cut(s) 55, 575
AccB7I CCANNNNNTGG 1 cut(s) 443
AccII CGCG 3 cut(s) 89, 147, 904
AclWI GGATC 2 cut(s) 283, 291
AcoI YGGCCR 2 cut(s) 84, 142
AcsI RAATTY 3 cut(s) 25, 893, 940
AcuI CTGAAG 1 cut(s) 560
AcyI GRCGYC 2 cut(s) 77, 150
AfaI GTAC 3 cut(s) 113, 577, 925
AfiI CCNNNNNNNGG 7 cut(s) 209, 443, 460, 494, 689, 863, 864
AflIII ACRYGT 1 cut(s) 321
AgsI TTSAA 4 cut(s) 368, 385, 638, 991
AhdI GACNNNNNGTC 1 cut(s) 206
AjnI CCWGG 3 cut(s) 442, 616, 948
AluBI AGCT 7 cut(s) 356, 364, 451, 649, 679, 814, 981
AluI AGCT 7 cut(s) 356, 364, 451, 649, 679, 814, 981
Alw21I GWGCWC 5 cut(s) 134, 358, 453, 468, 651
Alw26I GTCTC 2 cut(s) 236, 909
Alw44I GTGCAC 1 cut(s) 130
AlwI GGATC 2 cut(s) 283, 291
AoxI GGCC 3 cut(s) 84, 142, 946
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 3 cut(s) 161, 361, 662
ApoI RAATTY 3 cut(s) 25, 893, 940
Asp718I GGTACC 1 cut(s) 575
AspLEI GCGC 2 cut(s) 64, 708
AspS9I GGNCC 2 cut(s) 200, 407
AsuHPI GGTGA 4 cut(s) 9, 289, 458, 494
AvaII GGWCC 2 cut(s) 200, 407
BaeGI GKGCMC 1 cut(s) 134
BanI GGYRCC 2 cut(s) 55, 575
BanII GRGCYC 3 cut(s) 358, 453, 651
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 5 cut(s) 134, 358, 453, 468, 651
BbvI GCAGC 3 cut(s) 148, 373, 649
BccI CCATC 1 cut(s) 191
BceAI ACGGC 2 cut(s) 90, 112
BcgI CGANNNNNNTGC 2 cut(s) 584, 618
BciT130I CCWGG 3 cut(s) 444, 618, 950
BcoDI GTCTC 2 cut(s) 236, 909
BfaI CTAG 3 cut(s) 165, 171, 783
BfoI RGCGCY 2 cut(s) 65, 709
BglI GCCNNNNNGGC 1 cut(s) 158
BglII AGATCT 1 cut(s) 370
BisI GCNGC 8 cut(s) 87, 139, 142, 145, 162, 362, 663, 932
BlpI GCTNAGC 1 cut(s) 357
BlsI GCNGC 8 cut(s) 88, 140, 143, 146, 163, 363, 664, 933
Bme1390I CCNGG 3 cut(s) 444, 618, 950
Bme18I GGWCC 2 cut(s) 200, 407
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 200, 407
BmiI GGNNCC 2 cut(s) 57, 577
BmrFI CCNGG 3 cut(s) 444, 618, 950
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 2 cut(s) 633, 665
BpmI CTGGAG 1 cut(s) 971
Bpu10I CCTNAGC 1 cut(s) 36
Bpu1102I GCTNAGC 1 cut(s) 357
BpuEI CTTGAG 1 cut(s) 581
BsaHI GRCGYC 2 cut(s) 77, 150
BsaJI CCNNGG 4 cut(s) 87, 442, 488, 688
Bsc4I CCNNNNNNNGG 7 cut(s) 209, 443, 460, 494, 689, 863, 864
Bse118I RCCGGY 2 cut(s) 58, 152
BseBI CCWGG 3 cut(s) 444, 618, 950
BseDI CCNNGG 4 cut(s) 87, 442, 488, 688
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 7 cut(s) 209, 443, 460, 494, 689, 863, 864
BseMII CTCAG 3 cut(s) 371, 450, 539
BseRI GAGGAG 6 cut(s) 243, 246, 249, 252, 476, 927
BseSI GKGCMC 1 cut(s) 134
BseX3I CGGCCG 1 cut(s) 142
BseXI GCAGC 3 cut(s) 148, 373, 649
BsgI GTGCAG 1 cut(s) 683
Bsh1236I CGCG 3 cut(s) 89, 147, 904
Bsh1285I CGRYCG 1 cut(s) 145
BshFI GGCC 3 cut(s) 86, 144, 948
BshNI GGYRCC 2 cut(s) 55, 575
BsiEI CGRYCG 1 cut(s) 145
BsiHKAI GWGCWC 5 cut(s) 134, 358, 453, 468, 651
BsiSI CCGG 2 cut(s) 59, 153
BslFI GGGAC 1 cut(s) 865
BslI CCNNNNNNNGG 7 cut(s) 209, 443, 460, 494, 689, 863, 864
BsmAI GTCTC 2 cut(s) 236, 909
BsmBI CGTCTC 2 cut(s) 236, 909
BsmFI GGGAC 1 cut(s) 865
BsnI GGCC 3 cut(s) 86, 144, 948
Bsp1286I GDGCHC 5 cut(s) 134, 358, 453, 468, 651
Bsp1407I TGTACA 1 cut(s) 923
Bsp143I GATC 4 cut(s) 283, 288, 370, 774
Bsp1720I GCTNAGC 1 cut(s) 357
BspANI GGCC 3 cut(s) 86, 144, 948
BspCNI CTCAG 3 cut(s) 370, 451, 538
BspFNI CGCG 3 cut(s) 89, 147, 904
BspLI GGNNCC 2 cut(s) 57, 577
BspPI GGATC 2 cut(s) 283, 291
BspT107I GGYRCC 2 cut(s) 55, 575
BsrFI RCCGGY 2 cut(s) 58, 152
BsrGI TGTACA 1 cut(s) 923
BssAI RCCGGY 2 cut(s) 58, 152
BssECI CCNNGG 4 cut(s) 87, 442, 488, 688
BssMI GATC 4 cut(s) 283, 288, 370, 774
BssNI GRCGYC 2 cut(s) 77, 150
BssT1I CCWWGG 2 cut(s) 488, 688
Bst2UI CCWGG 3 cut(s) 444, 618, 950
Bst4CI ACNGT 5 cut(s) 21, 55, 473, 482, 632
BstACI GRCGYC 2 cut(s) 77, 150
BstAUI TGTACA 1 cut(s) 923
BstC8I GCNNGC 4 cut(s) 60, 496, 500, 983
BstDEI CTNAG 4 cut(s) 36, 357, 459, 525
BstDSI CCRYGG 1 cut(s) 87
BstENI CCTNNNNNAGG 3 cut(s) 458, 492, 687
BstF5I GGATG 1 cut(s) 271
BstFNI CGCG 3 cut(s) 89, 147, 904
BstH2I RGCGCY 2 cut(s) 65, 709
BstHHI GCGC 2 cut(s) 64, 708
BstKTI GATC 4 cut(s) 286, 291, 373, 777
BstMAI GTCTC 2 cut(s) 236, 909
BstMBI GATC 4 cut(s) 283, 288, 370, 774
BstMCI CGRYCG 1 cut(s) 145
BstMWI GCNNNNNNNGC 7 cut(s) 83, 138, 144, 158, 167, 176, 811
BstNI CCWGG 3 cut(s) 444, 618, 950
BstSCI CCNGG 3 cut(s) 442, 616, 948
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 3 cut(s) 89, 147, 904
BstV1I GCAGC 3 cut(s) 148, 373, 649
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 2 cut(s) 288, 370
BstYI RGATCY 2 cut(s) 288, 370
BstZI CGGCCG 1 cut(s) 142
BsuRI GGCC 3 cut(s) 86, 144, 948
BtgI CCRYGG 1 cut(s) 87
BtgZI GCGATG 1 cut(s) 204
BtsCI GGATG 1 cut(s) 271
BtsIMutI CAGTG 2 cut(s) 87, 628
Cac8I GCNNGC 4 cut(s) 60, 496, 500, 983
CciNI GCGGCCGC 1 cut(s) 142
CfoI GCGC 2 cut(s) 64, 708
Cfr10I RCCGGY 2 cut(s) 58, 152
Cfr13I GGNCC 2 cut(s) 200, 407
Cfr42I CCGCGG 1 cut(s) 90
CseI GACGC 2 cut(s) 66, 158
Csp6I GTAC 3 cut(s) 112, 576, 924
CviAII CATG 2 cut(s) 438, 733
CviQI GTAC 3 cut(s) 112, 576, 924
DdeI CTNAG 4 cut(s) 36, 357, 459, 525
DpnI GATC 4 cut(s) 285, 290, 372, 776
DpnII GATC 4 cut(s) 283, 288, 370, 774
DrdI GACNNNNNNGTC 2 cut(s) 250, 554
DriI GACNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 2 cut(s) 250, 554
EaeI YGGCCR 2 cut(s) 84, 142
EagI CGGCCG 1 cut(s) 142
Eam1105I GACNNNNNGTC 1 cut(s) 206
EciI GGCGGA 3 cut(s) 233, 240, 759
Ecl136II GAGCTC 3 cut(s) 356, 451, 649
EclXI CGGCCG 1 cut(s) 142
Eco130I CCWWGG 2 cut(s) 488, 688
Eco147I AGGCCT 1 cut(s) 948
Eco24I GRGCYC 3 cut(s) 358, 453, 651
Eco47I GGWCC 2 cut(s) 200, 407
Eco52I CGGCCG 1 cut(s) 142
Eco53kI GAGCTC 3 cut(s) 356, 451, 649
Eco57I CTGAAG 1 cut(s) 560
EcoICRI GAGCTC 3 cut(s) 356, 451, 649
EcoNI CCTNNNNNAGG 3 cut(s) 458, 492, 687
EcoO109I RGGNCCY 1 cut(s) 407
EcoRII CCWGG 3 cut(s) 442, 616, 948
EcoT14I CCWWGG 2 cut(s) 488, 688
EcoT38I GRGCYC 3 cut(s) 358, 453, 651
ErhI CCWWGG 2 cut(s) 488, 688
Esp3I CGTCTC 2 cut(s) 236, 909
FaeI CATG 2 cut(s) 441, 736
FaqI GGGAC 1 cut(s) 865
FatI CATG 2 cut(s) 437, 732
FauI CCCGC 1 cut(s) 491
FauNDI CATATG 2 cut(s) 309, 801
Fnu4HI GCNGC 8 cut(s) 87, 139, 142, 145, 162, 362, 663, 932
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 3 cut(s) 358, 453, 651
Fsp4HI GCNGC 8 cut(s) 87, 139, 142, 145, 162, 362, 663, 932
FspBI CTAG 3 cut(s) 165, 171, 783
GlaI GCGC 2 cut(s) 63, 707
GluI GCNGC 8 cut(s) 87, 139, 142, 145, 162, 362, 663, 932
GsuI CTGGAG 1 cut(s) 971
HaeII RGCGCY 2 cut(s) 65, 709
HaeIII GGCC 3 cut(s) 86, 144, 948
HapII CCGG 2 cut(s) 59, 153
HgaI GACGC 2 cut(s) 66, 158
HhaI GCGC 2 cut(s) 64, 708
Hin1I GRCGYC 2 cut(s) 77, 150
Hin1II CATG 2 cut(s) 441, 736
Hin6I GCGC 2 cut(s) 62, 706
HinP1I GCGC 2 cut(s) 62, 706
HindIII AAGCTT 1 cut(s) 812
HinfI GANTC 5 cut(s) 183, 413, 548, 585, 870
HpaII CCGG 2 cut(s) 59, 153
HphI GGTGA 4 cut(s) 9, 289, 458, 494
Hpy166II GTNNAC 1 cut(s) 132
Hpy188I TCNGA 8 cut(s) 72, 288, 293, 418, 540, 584, 757, 962
Hpy188III TCNNGA 2 cut(s) 368, 635
Hpy8I GTNNAC 1 cut(s) 132
Hpy99I CGWCG 4 cut(s) 34, 127, 152, 259
HpyAV CCTTC 6 cut(s) 253, 274, 325, 398, 693, 1021
HpyCH4III ACNGT 5 cut(s) 21, 55, 473, 482, 632
HpyCH4IV ACGT 1 cut(s) 321
HpyCH4V TGCA 5 cut(s) 132, 307, 313, 595, 700
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 138, 144, 158, 167, 176, 811
HpyF3I CTNAG 4 cut(s) 36, 357, 459, 525
HpySE526I ACGT 1 cut(s) 321
Hsp92I GRCGYC 2 cut(s) 77, 150
Hsp92II CATG 2 cut(s) 441, 736
HspAI GCGC 2 cut(s) 62, 706
KpnI GGTACC 1 cut(s) 579
KroI GCCGGC 1 cut(s) 58
KroNI GCCGGC 1 cut(s) 60
KspI CCGCGG 1 cut(s) 90
Kzo9I GATC 4 cut(s) 283, 288, 370, 774
LmnI GCTCC 2 cut(s) 456, 463
Lsp1109I GCAGC 3 cut(s) 148, 373, 649
MaeI CTAG 3 cut(s) 165, 171, 783
MaeII ACGT 1 cut(s) 321
MaeIII GTNAC 2 cut(s) 15, 473
MalI GATC 4 cut(s) 285, 290, 372, 776
MboI GATC 4 cut(s) 283, 288, 370, 774
MboII GAAGA 5 cut(s) 88, 91, 686, 788, 865
MfeI CAATTG 1 cut(s) 552
MflI RGATCY 2 cut(s) 288, 370
MhlI GDGCHC 5 cut(s) 134, 358, 453, 468, 651
MluCI AATT 5 cut(s) 25, 336, 552, 893, 940
MlyI GAGTC 3 cut(s) 192, 542, 594
MmeI TCCRAC 2 cut(s) 805, 940
MroNI GCCGGC 1 cut(s) 58
MseI TTAA 3 cut(s) 339, 612, 1033
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 59, 153
MspR9I CCNGG 3 cut(s) 444, 618, 950
MunI CAATTG 1 cut(s) 552
MvaI CCWGG 3 cut(s) 444, 618, 950
MvnI CGCG 3 cut(s) 89, 147, 904
MwoI GCNNNNNNNGC 7 cut(s) 83, 138, 144, 158, 167, 176, 811
NaeI GCCGGC 1 cut(s) 60
NdeI CATATG 2 cut(s) 309, 801
NdeII GATC 4 cut(s) 283, 288, 370, 774
NgoMIV GCCGGC 1 cut(s) 58
NlaIII CATG 2 cut(s) 441, 736
NlaIV GGNNCC 2 cut(s) 57, 577
NmuCI GTSAC 1 cut(s) 15
NotI GCGGCCGC 1 cut(s) 142
PceI AGGCCT 1 cut(s) 948
PcsI WCGNNNNNNNCGW 2 cut(s) 192, 251
PdiI GCCGGC 1 cut(s) 60
PfeI GAWTC 2 cut(s) 413, 870
PflFI GACNNNGTC 1 cut(s) 76
PflMI CCANNNNNTGG 1 cut(s) 443
PfoI TCCNGGA 1 cut(s) 616
PkrI GCNGC 8 cut(s) 88, 140, 143, 146, 163, 363, 664, 933
PleI GAGTC 3 cut(s) 191, 542, 593
PpsI GAGTC 3 cut(s) 191, 542, 593
PpuMI RGGWCCY 1 cut(s) 407
Psp124BI GAGCTC 3 cut(s) 358, 453, 651
Psp5II RGGWCCY 1 cut(s) 407
Psp6I CCWGG 3 cut(s) 442, 616, 948
PspGI CCWGG 3 cut(s) 442, 616, 948
PspN4I GGNNCC 2 cut(s) 57, 577
PspPI GGNCC 2 cut(s) 200, 407
PspPPI RGGWCCY 1 cut(s) 407
PsuI RGATCY 2 cut(s) 288, 370
PsyI GACNNNGTC 1 cut(s) 76
RsaI GTAC 3 cut(s) 113, 577, 925
RsaNI GTAC 3 cut(s) 112, 576, 924
SacI GAGCTC 3 cut(s) 358, 453, 651
SacII CCGCGG 1 cut(s) 90
SaqAI TTAA 3 cut(s) 339, 612, 1033
SatI GCNGC 8 cut(s) 87, 139, 142, 145, 162, 362, 663, 932
Sau3AI GATC 4 cut(s) 283, 288, 370, 774
Sau96I GGNCC 2 cut(s) 200, 407
SchI GAGTC 3 cut(s) 192, 542, 594
ScrFI CCNGG 3 cut(s) 444, 618, 950
SduI GDGCHC 5 cut(s) 134, 358, 453, 468, 651
Sfr303I CCGCGG 1 cut(s) 90
SgrAI CRCCGGYG 1 cut(s) 152
SgrBI CCGCGG 1 cut(s) 90
SinI GGWCC 2 cut(s) 200, 407
SmlI CTYRAG 1 cut(s) 560
SmoI CTYRAG 1 cut(s) 560
Sse9I AATT 5 cut(s) 25, 336, 552, 893, 940
SseBI AGGCCT 1 cut(s) 948
SspMI CTAG 3 cut(s) 165, 171, 783
SstI GAGCTC 3 cut(s) 358, 453, 651
StuI AGGCCT 1 cut(s) 948
StyD4I CCNGG 3 cut(s) 442, 616, 948
StyI CCWWGG 2 cut(s) 488, 688
TaaI ACNGT 5 cut(s) 21, 55, 473, 482, 632
TaiI ACGT 1 cut(s) 324
TaqI TCGA 3 cut(s) 195, 257, 1011
TaqII GACCGA 1 cut(s) 217
TasI AATT 5 cut(s) 25, 336, 552, 893, 940
TatI WGTACW 2 cut(s) 111, 923
TauI GCSGC 5 cut(s) 89, 141, 144, 147, 934
TfiI GAWTC 2 cut(s) 413, 870
Tru1I TTAA 3 cut(s) 339, 612, 1033
Tru9I TTAA 3 cut(s) 339, 612, 1033
TscAI CASTG 2 cut(s) 87, 635
TseFI GTSAC 1 cut(s) 15
TseI GCWGC 3 cut(s) 161, 361, 662
Tsp45I GTSAC 1 cut(s) 15
TspDTI ATGAA 3 cut(s) 417, 589, 788
TspRI CASTG 2 cut(s) 87, 635
Tth111I GACNNNGTC 1 cut(s) 76
Van91I CCANNNNNTGG 1 cut(s) 443
VneI GTGCAC 1 cut(s) 130
VpaK11BI GGWCC 2 cut(s) 200, 407
XagI CCTNNNNNAGG 3 cut(s) 458, 492, 687
XapI RAATTY 3 cut(s) 25, 893, 940
XspI CTAG 3 cut(s) 165, 171, 783
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.