RLG00000025596

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013395
Physical Location & Seq
Reverse (-)
46988577 .. 46990313
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025596

Sequence Viewer

Length: 1014 bp
ATGGCTCTCTCCTCGTCTTCGTCGTCACCGTCCAAGGGCATAGTGGTAACTGTCCCGGTACTGGTCCTCTCGGCCTCGGTCGCCGCCGTCTTCTTCTTCTTCCTCCTCTCCTCTCTCTCCTCTTGCTCCTGTGGCGGTGGTCGTGTCACCGTTGCTAGCATGCCGAGTCAGTCTGGCGGAGGAGATGTGGCTGCGGCGACCGGAAGGTGGGAGAGCGGCGAGACGATACGGTCGTCAAAGGAGGATGTGGAGTGGGTGAAGGATCAGATCCGAGTGAATGGGCTGCATATGCAGGATAACGTGTTGCGTAAGGGAATTAACCCTAGGACTCGGGCTCAGCAGCTCCAGGATCTCTTGCAGTACAAGGGTATATCTCACTATGAAGGACCTGACTCTGGAAATCACACTGCCCTCCCATGCCCTGGTGAGCTCCTTACTGAAGAACACCACAGTAACTATGGTGAGCCTTGGGCTGGCGGGCGAGATGTTTTTGAGTTCCTTGCTCAGTCCAGCCATCTTAAGCCTGATTCAAAGGTTCTTGAGATTGGCTGTGGTACCCTCCGAGTTGGTTTGCACTTCATTCGTTATCTTCATCCCGGACACTTCCACTGTCTCGAAAGAGATGAACTCTCACTAATGGCTGCCTTTAGATATGAGCTTCCTTCCCAAGGTCTTCTTCATAAGCGCCCTTTAATTGTGAAAGGTGAGAACATGGACTTCAGCAGATTTGGTTCTGACGTTGTGTATGATTTGATCTATGCTAGTGCTGTGTTTCTTCATATGCCTGGTGATCTTGTGTGGCTTGGGTTAGAGAGATTGACAAAGAAATTGAAACCTTATGATGGGCGGATCTTTGTGTCCCATAATATCAAGTTCTGCTCACGGTTGGGAGGGGAAGAGTGTACGAAACGGCTTACGAAGCTGGGCCTGGAGTATGTCGGAAAACATACACATGACAGCTTGCTTTTTAATCATTATGAGATCTGGTTTGAGTTTAAGCGGACAAAGGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_23

Protein Analysis

338

Amino Acids

37.5

Weight (kDa)

7.7

Isoelectric Point (pI)

48.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 180 - 283 6.5e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05100
fragaria_vesca FvH4_6g03940
malus_domestica MD04G1217100.v1.1 MD12G1234100.v1.1
prunus_persica Prupe.6G337200_v2.0.a1 Prupe.6G337200_v2.0.a1
pyrus_communis pycom04g19320 pycom12g21650 pycom16g23200
rosa_chinensis RchiOBHm_Chr3g0452511
rosa_laevigata RLG00000025596
rosa_roxburghii Rroxscaffold_6G00429540
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043200 Rh3BG044900 Rh3CG043500 Rh3DG044300
rosa_wichuraiana Rw3G003350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 229
Acc65I GGTACC 1 cut(s) 554
AccB1I GGYRCC 1 cut(s) 554
AccB7I CCANNNNNTGG 1 cut(s) 422
AccBSI CCGCTC 1 cut(s) 216
AciI CCGC 8 cut(s) 84, 135, 177, 194, 216, 477, 847, 1000
AclWI GGATC 4 cut(s) 262, 270, 357, 857
AcuI CTGAAG 2 cut(s) 459, 703
AfaI GTAC 4 cut(s) 60, 362, 556, 904
AfiI CCNNNNNNNGG 8 cut(s) 35, 61, 207, 395, 422, 473, 668, 842
AflII CTTAAG 1 cut(s) 518
AflIII ACRYGT 1 cut(s) 300
AgsI TTSAA 2 cut(s) 531, 832
AjnI CCWGG 4 cut(s) 345, 421, 784, 927
AjuI GAANNNNNNNTTGG 2 cut(s) 660, 692
AluBI AGCT 5 cut(s) 343, 430, 658, 922, 960
AluI AGCT 5 cut(s) 343, 430, 658, 922, 960
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 2 cut(s) 215, 617
AlwI GGATC 4 cut(s) 262, 270, 357, 857
Ama87I CYCGRG 1 cut(s) 330
AoxI GGCC 3 cut(s) 72, 925, 1008
ApeKI GCWGC 4 cut(s) 191, 283, 340, 641
Asp718I GGTACC 1 cut(s) 554
AspA2I CCTAGG 1 cut(s) 323
AspLEI GCGC 1 cut(s) 687
AspS9I GGNCC 3 cut(s) 64, 386, 925
AsuC2I CCSGG 2 cut(s) 56, 597
AsuHPI GGTGA 7 cut(s) 18, 139, 268, 437, 473, 716, 800
AsuNHI GCTAGC 1 cut(s) 155
AvaI CYCGRG 1 cut(s) 330
AvaII GGWCC 2 cut(s) 64, 386
AvrII CCTAGG 1 cut(s) 323
BaeI ACNNNNGTAYC 2 cut(s) 218, 251
BanI GGYRCC 1 cut(s) 554
BanII GRGCYC 2 cut(s) 337, 432
BbsI GAAGAC 3 cut(s) 9, 82, 665
Bbv12I GWGCWC 1 cut(s) 432
BbvI GCAGC 4 cut(s) 178, 270, 352, 628
BccI CCATC 2 cut(s) 522, 836
BceAI ACGGC 2 cut(s) 71, 926
BcgI CGANNNNNNTGC 2 cut(s) 563, 597
BciT130I CCWGG 4 cut(s) 347, 423, 786, 929
BcnI CCSGG 2 cut(s) 56, 597
BcoDI GTCTC 2 cut(s) 215, 617
BfaI CTAG 4 cut(s) 156, 324, 762, 1012
BfoI RGCGCY 1 cut(s) 688
BfrI CTTAAG 1 cut(s) 518
BglII AGATCT 1 cut(s) 981
BisI GCNGC 7 cut(s) 84, 192, 195, 217, 284, 341, 642
BlnI CCTAGG 1 cut(s) 323
BlpI GCTNAGC 1 cut(s) 336
BlsI GCNGC 7 cut(s) 85, 193, 196, 218, 285, 342, 643
Bme1390I CCNGG 6 cut(s) 56, 347, 423, 597, 786, 929
Bme18I GGWCC 2 cut(s) 64, 386
BmeT110I CYCGRG 1 cut(s) 330
BmgT120I GGNCC 3 cut(s) 64, 386, 925
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 6 cut(s) 56, 347, 423, 597, 786, 929
BmtI GCTAGC 1 cut(s) 159
BpiI GAAGAC 3 cut(s) 9, 82, 665
BplI GAGNNNNNCTC 2 cut(s) 612, 644
BpmI CTGGAG 2 cut(s) 329, 950
Bpu1102I GCTNAGC 1 cut(s) 336
BpuEI CTTGAG 1 cut(s) 560
BpuMI CCSGG 2 cut(s) 56, 597
BsaJI CCNNGG 6 cut(s) 33, 75, 323, 421, 467, 667
BsaWI WCCGGW 1 cut(s) 200
BsaXI ACNNNNNCTCC 2 cut(s) 171, 201
Bsc4I CCNNNNNNNGG 8 cut(s) 35, 61, 207, 395, 422, 473, 668, 842
Bse1I ACTGG 1 cut(s) 66
BseBI CCWGG 4 cut(s) 347, 423, 786, 929
BseDI CCNNGG 6 cut(s) 33, 75, 323, 421, 467, 667
BseGI GGATG 2 cut(s) 250, 592
BseLI CCNNNNNNNGG 8 cut(s) 35, 61, 207, 395, 422, 473, 668, 842
BseMII CTCAG 2 cut(s) 350, 518
BseNI ACTGG 1 cut(s) 66
BseRI GAGGAG 4 cut(s) 95, 100, 109, 195
BseXI GCAGC 4 cut(s) 178, 270, 352, 628
BseYI CCCAGC 1 cut(s) 922
Bsh1285I CGRYCG 3 cut(s) 81, 201, 233
BshFI GGCC 3 cut(s) 74, 927, 1010
BshNI GGYRCC 1 cut(s) 554
BsiEI CGRYCG 3 cut(s) 81, 201, 233
BsiHKAI GWGCWC 1 cut(s) 432
BsiHKCI CYCGRG 1 cut(s) 330
BsiSI CCGG 3 cut(s) 56, 201, 597
BslFI GGGAC 2 cut(s) 38, 844
BslI CCNNNNNNNGG 8 cut(s) 35, 61, 207, 395, 422, 473, 668, 842
BsmAI GTCTC 2 cut(s) 215, 617
BsmBI CGTCTC 1 cut(s) 215
BsmFI GGGAC 2 cut(s) 38, 844
BsnI GGCC 3 cut(s) 74, 927, 1010
BsoBI CYCGRG 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 337, 432
Bsp143I GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
Bsp1720I GCTNAGC 1 cut(s) 336
BspACI CCGC 8 cut(s) 84, 135, 177, 194, 216, 477, 847, 1000
BspANI GGCC 3 cut(s) 74, 927, 1010
BspCNI CTCAG 2 cut(s) 349, 517
BspLI GGNNCC 1 cut(s) 556
BspOI GCTAGC 1 cut(s) 159
BspPI GGATC 4 cut(s) 262, 270, 357, 857
BspT107I GGYRCC 1 cut(s) 554
BspTI CTTAAG 1 cut(s) 518
BsrBI CCGCTC 1 cut(s) 216
BsrI ACTGG 1 cut(s) 66
BssECI CCNNGG 6 cut(s) 33, 75, 323, 421, 467, 667
BssMI GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
BssT1I CCWWGG 4 cut(s) 33, 323, 467, 667
Bst2UI CCWGG 4 cut(s) 347, 423, 786, 929
Bst4CI ACNGT 7 cut(s) 30, 52, 151, 231, 452, 611, 885
Bst6I CTCTTC 1 cut(s) 891
BstAFI CTTAAG 1 cut(s) 518
BstC8I GCNNGC 5 cut(s) 157, 161, 475, 479, 962
BstDEI CTNAG 2 cut(s) 336, 504
BstENI CCTNNNNNAGG 1 cut(s) 666
BstF5I GGATG 2 cut(s) 250, 592
BstH2I RGCGCY 1 cut(s) 688
BstHHI GCGC 1 cut(s) 687
BstKTI GATC 7 cut(s) 265, 270, 352, 756, 793, 852, 984
BstMAI GTCTC 2 cut(s) 215, 617
BstMBI GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
BstMCI CGRYCG 3 cut(s) 81, 201, 233
BstMWI GCNNNNNNNGC 4 cut(s) 80, 132, 289, 919
BstNI CCWGG 4 cut(s) 347, 423, 786, 929
BstNSI RCATGY 1 cut(s) 163
BstSCI CCNGG 6 cut(s) 54, 345, 421, 595, 784, 927
BstV1I GCAGC 4 cut(s) 178, 270, 352, 628
BstV2I GAAGAC 3 cut(s) 9, 82, 665
BstX2I RGATCY 4 cut(s) 267, 349, 849, 981
BstYI RGATCY 4 cut(s) 267, 349, 849, 981
BsuRI GGCC 3 cut(s) 74, 927, 1010
BtsCI GGATG 2 cut(s) 250, 592
BtsI GCAGTG 1 cut(s) 405
BtsIMutI CAGTG 2 cut(s) 405, 607
Cac8I GCNNGC 5 cut(s) 157, 161, 475, 479, 962
CfoI GCGC 1 cut(s) 687
Cfr13I GGNCC 3 cut(s) 64, 386, 925
Csp6I GTAC 4 cut(s) 59, 361, 555, 903
CviAII CATG 4 cut(s) 160, 417, 712, 953
CviQI GTAC 4 cut(s) 59, 361, 555, 903
DdeI CTNAG 2 cut(s) 336, 504
DpnI GATC 7 cut(s) 264, 269, 351, 755, 792, 851, 983
DpnII GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
DrdI GACNNNNNNGTC 1 cut(s) 229
DseDI GACNNNNNNGTC 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 891
EarI CTCTTC 1 cut(s) 891
EciI GGCGGA 2 cut(s) 192, 862
Ecl136II GAGCTC 1 cut(s) 430
Eco130I CCWWGG 4 cut(s) 33, 323, 467, 667
Eco147I AGGCCT 1 cut(s) 1010
Eco24I GRGCYC 2 cut(s) 337, 432
Eco47I GGWCC 2 cut(s) 64, 386
Eco53kI GAGCTC 1 cut(s) 430
Eco57I CTGAAG 2 cut(s) 459, 703
Eco88I CYCGRG 1 cut(s) 330
EcoICRI GAGCTC 1 cut(s) 430
EcoNI CCTNNNNNAGG 1 cut(s) 666
EcoO109I RGGNCCY 1 cut(s) 386
EcoRII CCWGG 4 cut(s) 345, 421, 784, 927
EcoT14I CCWWGG 4 cut(s) 33, 323, 467, 667
EcoT38I GRGCYC 2 cut(s) 337, 432
ErhI CCWWGG 4 cut(s) 33, 323, 467, 667
Esp3I CGTCTC 1 cut(s) 215
FaeI CATG 4 cut(s) 163, 420, 715, 956
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 2 cut(s) 38, 844
FatI CATG 4 cut(s) 159, 416, 711, 952
FauI CCCGC 1 cut(s) 470
FauNDI CATATG 2 cut(s) 288, 780
Fnu4HI GCNGC 7 cut(s) 84, 192, 195, 217, 284, 341, 642
FokI GGATG 2 cut(s) 257, 579
FriOI GRGCYC 2 cut(s) 337, 432
Fsp4HI GCNGC 7 cut(s) 84, 192, 195, 217, 284, 341, 642
FspBI CTAG 4 cut(s) 156, 324, 762, 1012
GlaI GCGC 1 cut(s) 686
GluI GCNGC 7 cut(s) 84, 192, 195, 217, 284, 341, 642
GsaI CCCAGC 1 cut(s) 926
GsuI CTGGAG 2 cut(s) 329, 950
HaeII RGCGCY 1 cut(s) 688
HaeIII GGCC 3 cut(s) 74, 927, 1010
HapII CCGG 3 cut(s) 56, 201, 597
HhaI GCGC 1 cut(s) 687
Hin1II CATG 4 cut(s) 163, 420, 715, 956
Hin6I GCGC 1 cut(s) 685
HinP1I GCGC 1 cut(s) 685
HinfI GANTC 4 cut(s) 166, 328, 392, 527
HpaII CCGG 3 cut(s) 56, 201, 597
HphI GGTGA 7 cut(s) 18, 139, 268, 437, 473, 716, 800
Hpy166II GTNNAC 1 cut(s) 903
Hpy188I TCNGA 5 cut(s) 267, 272, 563, 736, 941
Hpy188III TCNNGA 3 cut(s) 396, 539, 614
Hpy8I GTNNAC 1 cut(s) 903
Hpy99I CGWCG 1 cut(s) 25
HpyAV CCTTC 4 cut(s) 198, 253, 377, 672
HpyCH4III ACNGT 7 cut(s) 30, 52, 151, 231, 452, 611, 885
HpyCH4IV ACGT 2 cut(s) 300, 738
HpyCH4V TGCA 4 cut(s) 286, 292, 358, 574
HpyF10VI GCNNNNNNNGC 4 cut(s) 80, 132, 289, 919
HpyF3I CTNAG 2 cut(s) 336, 504
HpySE526I ACGT 2 cut(s) 300, 738
Hsp92II CATG 4 cut(s) 163, 420, 715, 956
HspAI GCGC 1 cut(s) 685
KpnI GGTACC 1 cut(s) 558
Kzo9I GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
LmnI GCTCC 3 cut(s) 131, 348, 435
Lsp1109I GCAGC 4 cut(s) 178, 270, 352, 628
MaeI CTAG 4 cut(s) 156, 324, 762, 1012
MaeII ACGT 2 cut(s) 300, 738
MaeIII GTNAC 4 cut(s) 24, 46, 145, 452
MalI GATC 7 cut(s) 264, 269, 351, 755, 792, 851, 983
MbiI CCGCTC 1 cut(s) 216
MboI GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
MflI RGATCY 4 cut(s) 267, 349, 849, 981
MhlI GDGCHC 2 cut(s) 337, 432
MluCI AATT 3 cut(s) 315, 693, 827
MlyI GAGTC 3 cut(s) 175, 322, 386
MmeI TCCRAC 1 cut(s) 919
MseI TTAA 5 cut(s) 318, 519, 692, 969, 996
MslI CAYNNNNRTG 1 cut(s) 951
MspCI CTTAAG 1 cut(s) 518
MspI CCGG 3 cut(s) 56, 201, 597
MspR9I CCNGG 6 cut(s) 56, 347, 423, 597, 786, 929
MvaI CCWGG 4 cut(s) 347, 423, 786, 929
MwoI GCNNNNNNNGC 4 cut(s) 80, 132, 289, 919
NciI CCSGG 2 cut(s) 56, 597
NdeI CATATG 2 cut(s) 288, 780
NdeII GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
NheI GCTAGC 1 cut(s) 155
NlaIII CATG 4 cut(s) 163, 420, 715, 956
NlaIV GGNNCC 1 cut(s) 556
NmeAIII GCCGAG 2 cut(s) 50, 189
NmuCI GTSAC 2 cut(s) 24, 145
NspI RCATGY 1 cut(s) 163
PaeI GCATGC 1 cut(s) 163
PceI AGGCCT 1 cut(s) 1010
PcsI WCGNNNNNNNCGW 3 cut(s) 20, 26, 230
PfeI GAWTC 1 cut(s) 527
PflMI CCANNNNNTGG 1 cut(s) 422
PfoI TCCNGGA 2 cut(s) 345, 595
PkrI GCNGC 7 cut(s) 85, 193, 196, 218, 285, 342, 643
PleI GAGTC 3 cut(s) 174, 322, 386
PpsI GAGTC 3 cut(s) 174, 322, 386
PpuMI RGGWCCY 1 cut(s) 386
Psp124BI GAGCTC 1 cut(s) 432
Psp5II RGGWCCY 1 cut(s) 386
Psp6I CCWGG 4 cut(s) 345, 421, 784, 927
PspFI CCCAGC 1 cut(s) 922
PspGI CCWGG 4 cut(s) 345, 421, 784, 927
PspN4I GGNNCC 1 cut(s) 556
PspPI GGNCC 3 cut(s) 64, 386, 925
PspPPI RGGWCCY 1 cut(s) 386
PsuI RGATCY 4 cut(s) 267, 349, 849, 981
RsaI GTAC 4 cut(s) 60, 362, 556, 904
RsaNI GTAC 4 cut(s) 59, 361, 555, 903
RseI CAYNNNNRTG 1 cut(s) 951
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 5 cut(s) 318, 519, 692, 969, 996
SatI GCNGC 7 cut(s) 84, 192, 195, 217, 284, 341, 642
Sau3AI GATC 7 cut(s) 262, 267, 349, 753, 790, 849, 981
Sau96I GGNCC 3 cut(s) 64, 386, 925
SchI GAGTC 3 cut(s) 175, 322, 386
ScrFI CCNGG 6 cut(s) 56, 347, 423, 597, 786, 929
SduI GDGCHC 2 cut(s) 337, 432
SinI GGWCC 2 cut(s) 64, 386
SmiMI CAYNNNNRTG 1 cut(s) 951
SmlI CTYRAG 2 cut(s) 518, 539
SmoI CTYRAG 2 cut(s) 518, 539
SphI GCATGC 1 cut(s) 163
Sse9I AATT 3 cut(s) 315, 693, 827
SseBI AGGCCT 1 cut(s) 1010
SsiI CCGC 8 cut(s) 84, 135, 177, 194, 216, 477, 847, 1000
SspMI CTAG 4 cut(s) 156, 324, 762, 1012
SstI GAGCTC 1 cut(s) 432
StuI AGGCCT 1 cut(s) 1010
StyD4I CCNGG 6 cut(s) 54, 345, 421, 595, 784, 927
StyI CCWWGG 4 cut(s) 33, 323, 467, 667
TaaI ACNGT 7 cut(s) 30, 52, 151, 231, 452, 611, 885
TaiI ACGT 2 cut(s) 303, 741
TaqI TCGA 1 cut(s) 615
TaqII GACCGA 1 cut(s) 67
TasI AATT 3 cut(s) 315, 693, 827
TatI WGTACW 1 cut(s) 360
TauI GCSGC 3 cut(s) 86, 197, 219
TfiI GAWTC 1 cut(s) 527
Tru1I TTAA 5 cut(s) 318, 519, 692, 969, 996
Tru9I TTAA 5 cut(s) 318, 519, 692, 969, 996
TscAI CASTG 2 cut(s) 412, 614
TseFI GTSAC 2 cut(s) 24, 145
TseI GCWGC 4 cut(s) 191, 283, 340, 641
Tsp45I GTSAC 2 cut(s) 24, 145
TspDTI ATGAA 6 cut(s) 396, 568, 581, 639, 668, 767
TspRI CASTG 2 cut(s) 412, 614
Van91I CCANNNNNTGG 1 cut(s) 422
Vha464I CTTAAG 1 cut(s) 518
VpaK11BI GGWCC 2 cut(s) 64, 386
XagI CCTNNNNNAGG 1 cut(s) 666
XceI RCATGY 1 cut(s) 163
XcmI CCANNNNNNNNNTGG 2 cut(s) 40, 455
XmaJI CCTAGG 1 cut(s) 323
XspI CTAG 4 cut(s) 156, 324, 762, 1012
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.