pycom04g19320

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0013395
Physical Location & Seq
Forward (+)
21077904 .. 21079429
1526 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g19320.1

Sequence Viewer

Length: 786 bp
ATGGTCGTCGAAGGAGGATGTGGAGTGGGTGAAGGATCGATCCGAGTGAATGGGTTGCATATGCAGGATAACGTGTTGAGGAAGGGAATTAACCCTCGCACCCGAGCTCAGCAGCTTGAAGATCTCTTGCAATTCAAGGGTATATCTCACTATGAAGGACCTGATTTGGAAAATCATACTGCCCTCCCATGCCCTGGTGAGCTCCTAACTGAGGAGCACCACAGTAACTACGGTGAGCCTTGGGCAGGTGGGCGAGATGTTTTTGAGTTCCTCGCCCAGTCCTCTCATCTGAAGCCTGACTCCAATGTCCTTGAGATCGGTTGTGGTACCCTCCGAGTTGGTTTGCATTTCATTCGCTATCTTAATCCCGGACACTTCCACTGTCTTGAAAGAGATGAGCTCTCGCTAATGGCTGCCTTCAGATACGAGCTTCCTTCCCAAGGTCTTCTGCACAAGCGCCCTTTGATTGTGAAAGGTGACAGCATGGACTTCAGCAGATTTGGGTCCAATGTTGTGTATGACTTGATCTATACTAGTGCTGTGTTTCTTCATATGCCTGATAAGCTTGTGTGGGTTGGATTAGAGAGATTGACAAATAAACTGAAACCCTATGACGGGCGAATCTTCGTTTCCCATAATATCAAATTCTGTTCGCGGTTGGGAGGAGACGAGTGTACAAAGCGGCTCACGAGTTTAGGCCTGGAGTATGTCGGAAAGCATACACATGACAGCTTGCTGTTTAATCACTATGAAATCTGGTTCGAGTTTAGGCGGACAAAGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_23

Protein Analysis

262

Amino Acids

29.69

Weight (kDa)

6.56

Isoelectric Point (pI)

34.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_12 PF08242 104 - 206 5.8e-06 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G05100
fragaria_vesca FvH4_6g03940
malus_domestica MD04G1217100.v1.1 MD12G1234100.v1.1
prunus_persica Prupe.6G337200_v2.0.a1 Prupe.6G337200_v2.0.a1
pyrus_communis pycom04g19320 pycom12g21650 pycom16g23200
rosa_chinensis RchiOBHm_Chr3g0452511
rosa_laevigata RLG00000025596
rosa_roxburghii Rroxscaffold_6G00429540
rosa_rugosa Rorug02G0641200
rosa_samantha Rh3AG043200 Rh3BG044900 Rh3CG043500 Rh3DG044300
rosa_wichuraiana Rw3G003350

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 236
AasI GACNNNNNNGTC 1 cut(s) 305
Acc36I ACCTGC 1 cut(s) 236
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 194
AccII CGCG 1 cut(s) 655
AciI CCGC 3 cut(s) 655, 682, 772
AclWI GGATC 2 cut(s) 34, 43
AcsI RAATTY 1 cut(s) 644
AcuI CTGAAG 3 cut(s) 311, 403, 475
AfaI GTAC 2 cut(s) 328, 676
AfiI CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
AflIII ACRYGT 1 cut(s) 72
AgsI TTSAA 3 cut(s) 119, 136, 389
AhlI ACTAGT 1 cut(s) 533
AjnI CCWGG 2 cut(s) 193, 699
AluBI AGCT 7 cut(s) 107, 115, 202, 400, 430, 565, 732
AluI AGCT 7 cut(s) 107, 115, 202, 400, 430, 565, 732
Alw21I GWGCWC 4 cut(s) 109, 204, 219, 402
Alw26I GTCTC 1 cut(s) 660
AlwI GGATC 2 cut(s) 34, 43
Ama87I CYCGRG 1 cut(s) 102
AoxI GGCC 1 cut(s) 697
ApeKI GCWGC 2 cut(s) 112, 413
ApoI RAATTY 1 cut(s) 644
Asp718I GGTACC 1 cut(s) 326
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 2 cut(s) 158, 504
AsuC2I CCSGG 1 cut(s) 369
AsuHPI GGTGA 4 cut(s) 41, 209, 245, 488
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 2 cut(s) 158, 504
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 3 cut(s) 109, 204, 402
BauI CACGAG 1 cut(s) 688
BbsI GAAGAC 1 cut(s) 437
Bbv12I GWGCWC 4 cut(s) 109, 204, 219, 402
BbvI GCAGC 2 cut(s) 124, 400
BcgI CGANNNNNNTGC 2 cut(s) 335, 369
BciT130I CCWGG 2 cut(s) 195, 701
BcnI CCSGG 1 cut(s) 369
BcoDI GTCTC 1 cut(s) 660
BcuI ACTAGT 1 cut(s) 533
BfaI CTAG 1 cut(s) 534
BfoI RGCGCY 1 cut(s) 460
BfuAI ACCTGC 1 cut(s) 236
BglII AGATCT 1 cut(s) 121
BisI GCNGC 3 cut(s) 113, 414, 683
BlpI GCTNAGC 1 cut(s) 108
BlsI GCNGC 3 cut(s) 114, 415, 684
Bme1390I CCNGG 3 cut(s) 195, 369, 701
Bme18I GGWCC 2 cut(s) 158, 504
BmeT110I CYCGRG 1 cut(s) 102
BmgT120I GGNCC 2 cut(s) 158, 504
BmiI GGNNCC 2 cut(s) 328, 505
BmrFI CCNGG 3 cut(s) 195, 369, 701
BmrI ACTGGG 1 cut(s) 271
BmuI ACTGGG 1 cut(s) 271
BpiI GAAGAC 1 cut(s) 437
BplI GAGNNNNNCTC 2 cut(s) 384, 416
BpmI CTGGAG 1 cut(s) 722
Bpu1102I GCTNAGC 1 cut(s) 108
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 369
Bsa29I ATCGAT 1 cut(s) 38
BsaJI CCNNGG 3 cut(s) 193, 239, 439
Bsc4I CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
Bse1I ACTGG 1 cut(s) 277
BseBI CCWGG 2 cut(s) 195, 701
BseCI ATCGAT 1 cut(s) 38
BseDI CCNNGG 3 cut(s) 193, 239, 439
BseGI GGATG 1 cut(s) 23
BseLI CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
BseMII CTCAG 2 cut(s) 122, 201
BseNI ACTGG 1 cut(s) 277
BseRI GAGGAG 2 cut(s) 227, 678
BseXI GCAGC 2 cut(s) 124, 400
BsgI GTGCAG 1 cut(s) 434
Bsh1236I CGCG 1 cut(s) 655
BshFI GGCC 1 cut(s) 699
BshNI GGYRCC 1 cut(s) 326
BshVI ATCGAT 1 cut(s) 38
BsiHKAI GWGCWC 4 cut(s) 109, 204, 219, 402
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 1 cut(s) 369
BslI CCNNNNNNNGG 6 cut(s) 194, 211, 245, 440, 614, 615
BsmAI GTCTC 1 cut(s) 660
BsmBI CGTCTC 1 cut(s) 660
BsnI GGCC 1 cut(s) 699
BsoBI CYCGRG 1 cut(s) 102
Bsp1286I GDGCHC 4 cut(s) 109, 204, 219, 402
Bsp1407I TGTACA 1 cut(s) 674
Bsp143I GATC 5 cut(s) 35, 39, 121, 315, 525
Bsp1720I GCTNAGC 1 cut(s) 108
BspACI CCGC 3 cut(s) 655, 682, 772
BspANI GGCC 1 cut(s) 699
BspCNI CTCAG 2 cut(s) 121, 202
BspDI ATCGAT 1 cut(s) 38
BspFNI CGCG 1 cut(s) 655
BspLI GGNNCC 2 cut(s) 328, 505
BspMI ACCTGC 1 cut(s) 236
BspPI GGATC 2 cut(s) 34, 43
BspT107I GGYRCC 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 674
BsrI ACTGG 1 cut(s) 277
BssECI CCNNGG 3 cut(s) 193, 239, 439
BssMI GATC 5 cut(s) 35, 39, 121, 315, 525
BssSI CACGAG 1 cut(s) 688
BssT1I CCWWGG 2 cut(s) 239, 439
Bst2BI CACGAG 1 cut(s) 688
Bst2UI CCWGG 2 cut(s) 195, 701
Bst4CI ACNGT 3 cut(s) 224, 233, 383
BstAUI TGTACA 1 cut(s) 674
BstC8I GCNNGC 1 cut(s) 734
BstDEI CTNAG 3 cut(s) 108, 210, 783
BstENI CCTNNNNNAGG 3 cut(s) 209, 243, 438
BstF5I GGATG 1 cut(s) 23
BstFNI CGCG 1 cut(s) 655
BstH2I RGCGCY 1 cut(s) 460
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 5 cut(s) 38, 42, 124, 318, 528
BstMAI GTCTC 1 cut(s) 660
BstMBI GATC 5 cut(s) 35, 39, 121, 315, 525
BstMWI GCNNNNNNNGC 1 cut(s) 562
BstNI CCWGG 2 cut(s) 195, 701
BstSCI CCNGG 3 cut(s) 193, 367, 699
BstUI CGCG 1 cut(s) 655
BstV1I GCAGC 2 cut(s) 124, 400
BstV2I GAAGAC 1 cut(s) 437
BstX2I RGATCY 1 cut(s) 121
BstYI RGATCY 1 cut(s) 121
Bsu15I ATCGAT 1 cut(s) 38
BsuRI GGCC 1 cut(s) 699
BsuTUI ATCGAT 1 cut(s) 38
BtsCI GGATG 1 cut(s) 23
BtsIMutI CAGTG 1 cut(s) 379
BveI ACCTGC 1 cut(s) 236
Cac8I GCNNGC 1 cut(s) 734
CfoI GCGC 1 cut(s) 459
Cfr13I GGNCC 2 cut(s) 158, 504
ClaI ATCGAT 1 cut(s) 38
Csp6I GTAC 2 cut(s) 327, 675
CviAII CATG 3 cut(s) 189, 484, 725
CviQI GTAC 2 cut(s) 327, 675
DdeI CTNAG 3 cut(s) 108, 210, 783
DpnI GATC 5 cut(s) 37, 41, 123, 317, 527
DpnII GATC 5 cut(s) 35, 39, 121, 315, 525
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
Ecl136II GAGCTC 3 cut(s) 107, 202, 400
Eco130I CCWWGG 2 cut(s) 239, 439
Eco147I AGGCCT 1 cut(s) 699
Eco24I GRGCYC 3 cut(s) 109, 204, 402
Eco47I GGWCC 2 cut(s) 158, 504
Eco53kI GAGCTC 3 cut(s) 107, 202, 400
Eco57I CTGAAG 3 cut(s) 311, 403, 475
Eco88I CYCGRG 1 cut(s) 102
EcoICRI GAGCTC 3 cut(s) 107, 202, 400
EcoNI CCTNNNNNAGG 3 cut(s) 209, 243, 438
EcoO109I RGGNCCY 1 cut(s) 158
EcoRII CCWGG 2 cut(s) 193, 699
EcoT14I CCWWGG 2 cut(s) 239, 439
EcoT38I GRGCYC 3 cut(s) 109, 204, 402
ErhI CCWWGG 2 cut(s) 239, 439
Esp3I CGTCTC 1 cut(s) 660
FaeI CATG 3 cut(s) 192, 487, 728
FatI CATG 3 cut(s) 188, 483, 724
FauNDI CATATG 2 cut(s) 60, 552
Fnu4HI GCNGC 3 cut(s) 113, 414, 683
FokI GGATG 1 cut(s) 30
FriOI GRGCYC 3 cut(s) 109, 204, 402
Fsp4HI GCNGC 3 cut(s) 113, 414, 683
FspBI CTAG 1 cut(s) 534
GlaI GCGC 1 cut(s) 458
GluI GCNGC 3 cut(s) 113, 414, 683
GsuI CTGGAG 1 cut(s) 722
HaeII RGCGCY 1 cut(s) 460
HaeIII GGCC 1 cut(s) 699
HapII CCGG 1 cut(s) 369
HhaI GCGC 1 cut(s) 459
Hin1II CATG 3 cut(s) 192, 487, 728
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HindIII AAGCTT 1 cut(s) 563
HinfI GANTC 2 cut(s) 299, 621
HpaII CCGG 1 cut(s) 369
HphI GGTGA 4 cut(s) 41, 209, 245, 488
Hpy166II GTNNAC 1 cut(s) 675
Hpy188I TCNGA 5 cut(s) 44, 291, 335, 422, 713
Hpy188III TCNNGA 2 cut(s) 386, 688
Hpy8I GTNNAC 1 cut(s) 675
Hpy99I CGWCG 1 cut(s) 11
HpyAV CCTTC 6 cut(s) 5, 26, 76, 149, 427, 444
HpyCH4III ACNGT 3 cut(s) 224, 233, 383
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 5 cut(s) 58, 64, 130, 346, 451
HpyF10VI GCNNNNNNNGC 1 cut(s) 562
HpyF3I CTNAG 3 cut(s) 108, 210, 783
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 3 cut(s) 192, 487, 728
HspAI GCGC 1 cut(s) 457
KpnI GGTACC 1 cut(s) 330
Kzo9I GATC 5 cut(s) 35, 39, 121, 315, 525
LmnI GCTCC 2 cut(s) 207, 214
Lsp1109I GCAGC 2 cut(s) 124, 400
MaeI CTAG 1 cut(s) 534
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 2 cut(s) 224, 476
MalI GATC 5 cut(s) 37, 41, 123, 317, 527
MboI GATC 5 cut(s) 35, 39, 121, 315, 525
MboII GAAGA 4 cut(s) 131, 437, 539, 616
MflI RGATCY 1 cut(s) 121
MhlI GDGCHC 4 cut(s) 109, 204, 219, 402
MluCI AATT 3 cut(s) 87, 131, 644
MlyI GAGTC 1 cut(s) 293
MmeI TCCRAC 2 cut(s) 556, 691
MnlI CCTC 9 cut(s) 8, 72, 105, 194, 205, 281, 292, 341, 656
MseI TTAA 3 cut(s) 90, 363, 741
MslI CAYNNNNRTG 1 cut(s) 723
MspI CCGG 1 cut(s) 369
MspR9I CCNGG 3 cut(s) 195, 369, 701
MvaI CCWGG 2 cut(s) 195, 701
MvnI CGCG 1 cut(s) 655
MwoI GCNNNNNNNGC 1 cut(s) 562
NciI CCSGG 1 cut(s) 369
NdeI CATATG 2 cut(s) 60, 552
NdeII GATC 5 cut(s) 35, 39, 121, 315, 525
NlaIII CATG 3 cut(s) 192, 487, 728
NlaIV GGNNCC 2 cut(s) 328, 505
NmuCI GTSAC 1 cut(s) 476
PaqCI CACCTGC 1 cut(s) 236
PceI AGGCCT 1 cut(s) 699
PfeI GAWTC 1 cut(s) 621
PflMI CCANNNNNTGG 1 cut(s) 194
PfoI TCCNGGA 1 cut(s) 367
PkrI GCNGC 3 cut(s) 114, 415, 684
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
PpuMI RGGWCCY 1 cut(s) 158
Psp124BI GAGCTC 3 cut(s) 109, 204, 402
Psp5II RGGWCCY 1 cut(s) 158
Psp6I CCWGG 2 cut(s) 193, 699
PspGI CCWGG 2 cut(s) 193, 699
PspN4I GGNNCC 2 cut(s) 328, 505
PspPI GGNCC 2 cut(s) 158, 504
PspPPI RGGWCCY 1 cut(s) 158
PsuI RGATCY 1 cut(s) 121
RsaI GTAC 2 cut(s) 328, 676
RsaNI GTAC 2 cut(s) 327, 675
RseI CAYNNNNRTG 1 cut(s) 723
SacI GAGCTC 3 cut(s) 109, 204, 402
SaqAI TTAA 3 cut(s) 90, 363, 741
SatI GCNGC 3 cut(s) 113, 414, 683
Sau3AI GATC 5 cut(s) 35, 39, 121, 315, 525
Sau96I GGNCC 2 cut(s) 158, 504
SchI GAGTC 1 cut(s) 293
ScrFI CCNGG 3 cut(s) 195, 369, 701
SduI GDGCHC 4 cut(s) 109, 204, 219, 402
SinI GGWCC 2 cut(s) 158, 504
SmiMI CAYNNNNRTG 1 cut(s) 723
SmlI CTYRAG 1 cut(s) 311
SmoI CTYRAG 1 cut(s) 311
SpeI ACTAGT 1 cut(s) 533
Sse9I AATT 3 cut(s) 87, 131, 644
SseBI AGGCCT 1 cut(s) 699
SsiI CCGC 3 cut(s) 655, 682, 772
SspMI CTAG 1 cut(s) 534
SstI GAGCTC 3 cut(s) 109, 204, 402
StuI AGGCCT 1 cut(s) 699
StyD4I CCNGG 3 cut(s) 193, 367, 699
StyI CCWWGG 2 cut(s) 239, 439
TaaI ACNGT 3 cut(s) 224, 233, 383
TaiI ACGT 1 cut(s) 75
TaqI TCGA 3 cut(s) 9, 38, 762
TasI AATT 3 cut(s) 87, 131, 644
TatI WGTACW 1 cut(s) 674
TauI GCSGC 1 cut(s) 685
TfiI GAWTC 1 cut(s) 621
Tru1I TTAA 3 cut(s) 90, 363, 741
Tru9I TTAA 3 cut(s) 90, 363, 741
TscAI CASTG 1 cut(s) 386
TseFI GTSAC 1 cut(s) 476
TseI GCWGC 2 cut(s) 112, 413
Tsp45I GTSAC 1 cut(s) 476
TspDTI ATGAA 4 cut(s) 168, 340, 539, 765
TspRI CASTG 1 cut(s) 386
Van91I CCANNNNNTGG 1 cut(s) 194
VpaK11BI GGWCC 2 cut(s) 158, 504
XagI CCTNNNNNAGG 3 cut(s) 209, 243, 438
XapI RAATTY 1 cut(s) 644
XspI CTAG 1 cut(s) 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.