Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0006014

Physical Location & Seq

Genomic Coordinates Reverse (-)

32279690 .. 32283689

Gene Structure 4000 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1248400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1110 bp

ATGCTGAGCAGAACCACAACAACAGTGATAGCTGCGGCTTCTGCGAAGAGTTCCCAGTTGTTTGGGTTTGGGATCAACAACAGTAGCAGCAACAATCTCTGTTGCACTCGTACTACTACTACTCGTACAAGTAATCTCTCATGGAACTTCATCAACAGCAACAAGCACAAGTGTAGGGAGTCAAGAATGGAGGAGAGTTTCACCACCCGGGCCTCCGCGCAGCCTCTCACGAATGCCGATGACCTCATTGACTCTGTAGAGACCTTCATCTTCGACTGCGACGGTGTTATATGGAAAGGAGATAAGCTCATAGACGGTGTCCCCCAAACTCTGGATATGCTACGGTCTAAGGGCAAAAGATTAGTTTTCGTTACCAACAACTCAACAAAGTCTAGGAAGCAGTATGGTAAAAAGTTTGAGACGCTTGGTCTAAATGTCAATGAGGAGGAAATTTTTGCGTCATCTTTTGCTGCTGCTGCTTATTTGAAGTCCATTAATTTCCCAAAAGATAAAAAGGTATATGTGATTGGTGAGGAAGGTATCTTGAAGGAGCTTGAGCTTGCTGGATATGAGTACCTTGGTGGCCCAGAAGATGGTGGGAAGAAAATAGAGCTGAAGCCTGGATATCTGATGGAGCATGATGAGAGTGTTGGAGCTGTTGTGGTTGGATTTGATCGAAATTTCAACTACTACAAAATTCAGTATGGGACACTCTGTGTACGTGAAAACCCTGGATGTCTTTTCATTGCTACAAATCGTGATGCTGTCACTCATCTCACAGATGCTCAGGAATGGGCAGGTGGTGGTTCAATGGTTGGTGCTATCCGTGGATCAACTCAACGTGAGCCATTGGTTGTGGGAAAACCCTCAACTTTTATGATGGACTACTTAGCAAATGAATTTGGCATCCTGAAGTCACAGATTTGCATGGTTGGGGACAGATTAGATACTGATATTCTGTTTGGACAAAATGGTGGTTGCAAAACTCTTCTTGTTCTCTCGGGCGTCACCACCTTACCAGTGCTTCAGAGTCCCGACAACTCCATACAACCAGATTTTTACACGAACAAGATTTCAGACTTTTTGTTTCTCAAGGCTGCAACTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01130 ko01200 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

Hydrolase_6 Hydrolase_like

Protein Analysis

370

Amino Acids

40.6

Weight (kDa)

6.05

Isoelectric Point (pI)

29.04

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	87 - 326	3.5e-13	haloacid dehalogenase-like hydrolase
Hydrolase_6	PF13344	89 - 191	1e-34	Haloacid dehalogenase-like hydrolase
Hydrolase_like	PF13242	286 - 360	1.9e-20	HAD-hyrolase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006014)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36700 AT5G36700 AT5G36700 AT5G36700 AT5G36790 AT5G36790 AT5G36790
fragaria_vesca	FvH4_6g00110 FvH4_6g00110
malus_domestica	MD04G1248400.v1.1 MD12G1262300.v1.1
prunus_persica	Prupe.6G364700_v2.0.a1 Prupe.6G364700_v2.0.a1 Prupe.6G364700_v2.0.a1 Prupe.6G364700_v2.0.a1
pyrus_communis	pycom04g21910 pycom12g24170
rosa_chinensis	RchiOBHm_Chr3g0447461
rosa_laevigata	RLG00000025996
rosa_roxburghii	Rroxscaffold_6G00425680
rosa_rugosa	Rorug02G0601900 Rorug02G0602000
rosa_samantha	Rh3AG002200 Rh3BG001700 Rh3CG002000 Rh3DG002100
rosa_wichuraiana	Rw3G000110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	788
Acc36I	ACCTGC	1 cut(s)	788
AccB7I	CCANNNNNTGG	2 cut(s)	331, 593
AccII	CGCG	1 cut(s)	218
AciI	CCGC	2 cut(s)	35, 216
AclWI	GGATC	2 cut(s)	80, 838
AcsI	RAATTY	4 cut(s)	450, 679, 696, 899
AcuI	CTGAAG	3 cut(s)	635, 932, 1010
AcyI	GRCGYC	1 cut(s)	1005
AdeI	CACNNNGTG	1 cut(s)	716
AfaI	GTAC	4 cut(s)	112, 127, 575, 720
AfiI	CCNNNNNNNGG	2 cut(s)	331, 593
AgsI	TTSAA	4 cut(s)	487, 547, 685, 810
AhdI	GACNNNNNGTC	1 cut(s)	426
AjnI	CCWGG	2 cut(s)	619, 730
AluBI	AGCT	6 cut(s)	32, 307, 553, 559, 613, 656
AluI	AGCT	6 cut(s)	32, 307, 553, 559, 613, 656
Alw26I	GTCTC	2 cut(s)	254, 413
AlwI	GGATC	2 cut(s)	80, 838
Ama87I	CYCGRG	2 cut(s)	207, 1000
AoxI	GGCC	2 cut(s)	210, 583
ApeKI	GCWGC	7 cut(s)	32, 87, 220, 470, 473, 476, 1097
ApoI	RAATTY	4 cut(s)	450, 679, 696, 899
AseI	ATTAAT	1 cut(s)	495
AspLEI	GCGC	1 cut(s)	220
AspS9I	GGNCC	2 cut(s)	210, 584
AsuC2I	CCSGG	2 cut(s)	208, 209
AsuHPI	GGTGA	3 cut(s)	193, 542, 1000
AvaI	CYCGRG	2 cut(s)	207, 1000
BbvI	GCAGC	7 cut(s)	19, 99, 232, 457, 460, 463, 1084
BccI	CCATC	3 cut(s)	587, 625, 874
BciT130I	CCWGG	2 cut(s)	621, 732
BcnI	CCSGG	2 cut(s)	208, 209
BcoDI	GTCTC	2 cut(s)	254, 413
BfaI	CTAG	1 cut(s)	393
BfmI	CTRYAG	1 cut(s)	255
BfuAI	ACCTGC	1 cut(s)	788
BisI	GCNGC	8 cut(s)	33, 36, 88, 221, 471, 474, 477, 1098
BlpI	GCTNAGC	1 cut(s)	5
BlsI	GCNGC	8 cut(s)	34, 37, 89, 222, 472, 475, 478, 1099
Bme1390I	CCNGG	4 cut(s)	208, 209, 621, 732
BmeRI	GACNNNNNGTC	1 cut(s)	426
BmeT110I	CYCGRG	2 cut(s)	207, 1000
BmgT120I	GGNCC	2 cut(s)	210, 584
BmrFI	CCNGG	4 cut(s)	208, 209, 621, 732
BmrI	ACTGGG	1 cut(s)	49
BmsI	GCATC	3 cut(s)	751, 772, 915
BmuI	ACTGGG	1 cut(s)	49
BplI	GAGNNNNNCTC	2 cut(s)	291, 323
Bpu10I	CCTNAGC	1 cut(s)	786
Bpu1102I	GCTNAGC	1 cut(s)	5
BpuEI	CTTGAG	2 cut(s)	575, 1076
BpuMI	CCSGG	2 cut(s)	208, 209
BsaAI	YACGTR	1 cut(s)	722
BsaHI	GRCGYC	1 cut(s)	1005
BsaI	GGTCTC	1 cut(s)	254
BsaJI	CCNNGG	4 cut(s)	207, 577, 730, 826
BsaXI	ACNNNNNCTCC	2 cut(s)	645, 675
Bsc4I	CCNNNNNNNGG	2 cut(s)	331, 593
Bse1I	ACTGG	2 cut(s)	55, 1019
Bse3DI	GCAATG	1 cut(s)	744
BseBI	CCWGG	2 cut(s)	621, 732
BseDI	CCNNGG	4 cut(s)	207, 577, 730, 826
BseGI	GGATG	2 cut(s)	740, 906
BseLI	CCNNNNNNNGG	2 cut(s)	331, 593
BseMI	GCAATG	1 cut(s)	744
BseMII	CTCAG	1 cut(s)	800
BseNI	ACTGG	2 cut(s)	55, 1019
BseRI	GAGGAG	2 cut(s)	206, 458
BseXI	GCAGC	7 cut(s)	19, 99, 232, 457, 460, 463, 1084
Bsh1236I	CGCG	1 cut(s)	218
BshFI	GGCC	2 cut(s)	212, 585
BsiHKCI	CYCGRG	2 cut(s)	207, 1000
BsiSI	CCGG	1 cut(s)	208
BslFI	GGGAC	4 cut(s)	305, 721, 950, 1017
BslI	CCNNNNNNNGG	2 cut(s)	331, 593
BsmAI	GTCTC	2 cut(s)	254, 413
BsmBI	CGTCTC	1 cut(s)	413
BsmFI	GGGAC	4 cut(s)	305, 721, 950, 1017
BsmI	GAATGC	1 cut(s)	238
BsnI	GGCC	2 cut(s)	212, 585
Bso31I	GGTCTC	1 cut(s)	254
BsoBI	CYCGRG	2 cut(s)	207, 1000
Bsp143I	GATC	3 cut(s)	72, 673, 830
Bsp1720I	GCTNAGC	1 cut(s)	5
BspACI	CCGC	2 cut(s)	35, 216
BspANI	GGCC	2 cut(s)	212, 585
BspCNI	CTCAG	1 cut(s)	799
BspFNI	CGCG	1 cut(s)	218
BspMI	ACCTGC	1 cut(s)	788
BspPI	GGATC	2 cut(s)	80, 838
BspTNI	GGTCTC	1 cut(s)	254
BsrDI	GCAATG	1 cut(s)	744
BsrI	ACTGG	2 cut(s)	55, 1019
BssECI	CCNNGG	4 cut(s)	207, 577, 730, 826
BssMI	GATC	3 cut(s)	72, 673, 830
BssNI	GRCGYC	1 cut(s)	1005
BssT1I	CCWWGG	1 cut(s)	577
Bst2UI	CCWGG	2 cut(s)	621, 732
Bst4CI	ACNGT	6 cut(s)	25, 83, 284, 317, 345, 1105
Bst6I	CTCTTC	2 cut(s)	41, 993
BstACI	GRCGYC	1 cut(s)	1005
BstBAI	YACGTR	1 cut(s)	722
BstC8I	GCNNGC	1 cut(s)	561
BstDEI	CTNAG	4 cut(s)	5, 348, 786, 889
BstDSI	CCRYGG	1 cut(s)	826
BstF5I	GGATG	2 cut(s)	740, 906
BstFNI	CGCG	1 cut(s)	218
BstHHI	GCGC	1 cut(s)	220
BstKTI	GATC	3 cut(s)	75, 676, 833
BstMAI	GTCTC	2 cut(s)	254, 413
BstMBI	GATC	3 cut(s)	72, 673, 830
BstMWI	GCNNNNNNNGC	2 cut(s)	41, 476
BstNI	CCWGG	2 cut(s)	621, 732
BstSCI	CCNGG	4 cut(s)	206, 207, 619, 730
BstSFI	CTRYAG	1 cut(s)	255
BstUI	CGCG	1 cut(s)	218
BstV1I	GCAGC	7 cut(s)	19, 99, 232, 457, 460, 463, 1084
BstXI	CCANNNNNNTGG	1 cut(s)	62
BsuRI	GGCC	2 cut(s)	212, 585
BtgI	CCRYGG	1 cut(s)	826
BtsCI	GGATG	2 cut(s)	740, 906
BtsIMutI	CAGTG	2 cut(s)	30, 1026
BveI	ACCTGC	1 cut(s)	788
Cac8I	GCNNGC	1 cut(s)	561
CfoI	GCGC	1 cut(s)	220
Cfr13I	GGNCC	2 cut(s)	210, 584
Cfr9I	CCCGGG	1 cut(s)	207
CseI	GACGC	3 cut(s)	430, 447, 994
Csp6I	GTAC	4 cut(s)	111, 126, 574, 719
CviAII	CATG	3 cut(s)	141, 638, 928
CviQI	GTAC	4 cut(s)	111, 126, 574, 719
DdeI	CTNAG	4 cut(s)	5, 348, 786, 889
DpnI	GATC	3 cut(s)	74, 675, 832
DpnII	GATC	3 cut(s)	72, 673, 830
DraIII	CACNNNGTG	1 cut(s)	716
DriI	GACNNNNNGTC	1 cut(s)	426
Eam1104I	CTCTTC	2 cut(s)	41, 993
Eam1105I	GACNNNNNGTC	1 cut(s)	426
EarI	CTCTTC	2 cut(s)	41, 993
Eco130I	CCWWGG	1 cut(s)	577
Eco31I	GGTCTC	1 cut(s)	254
Eco32I	GATATC	1 cut(s)	626
Eco57I	CTGAAG	3 cut(s)	635, 932, 1010
Eco88I	CYCGRG	2 cut(s)	207, 1000
EcoRII	CCWGG	2 cut(s)	619, 730
EcoRV	GATATC	1 cut(s)	626
EcoT14I	CCWWGG	1 cut(s)	577
ErhI	CCWWGG	1 cut(s)	577
Esp3I	CGTCTC	1 cut(s)	413
FaeI	CATG	3 cut(s)	144, 641, 931
FaqI	GGGAC	4 cut(s)	305, 721, 950, 1017
FatI	CATG	3 cut(s)	140, 637, 927
Fnu4HI	GCNGC	8 cut(s)	33, 36, 88, 221, 471, 474, 477, 1098
FokI	GGATG	2 cut(s)	747, 893
Fsp4HI	GCNGC	8 cut(s)	33, 36, 88, 221, 471, 474, 477, 1098
FspBI	CTAG	1 cut(s)	393
GlaI	GCGC	1 cut(s)	219
GluI	GCNGC	8 cut(s)	33, 36, 88, 221, 471, 474, 477, 1098
HaeIII	GGCC	2 cut(s)	212, 585
HapII	CCGG	1 cut(s)	208
HgaI	GACGC	3 cut(s)	430, 447, 994
HhaI	GCGC	1 cut(s)	220
Hin1I	GRCGYC	1 cut(s)	1005
Hin1II	CATG	3 cut(s)	144, 641, 931
Hin6I	GCGC	1 cut(s)	218
HinP1I	GCGC	1 cut(s)	218
HinfI	GANTC	3 cut(s)	179, 251, 1030
HpaII	CCGG	1 cut(s)	208
HphI	GGTGA	3 cut(s)	193, 542, 1000
Hpy166II	GTNNAC	1 cut(s)	719
Hpy188I	TCNGA	3 cut(s)	630, 1029, 1078
Hpy188III	TCNNGA	8 cut(s)	183, 229, 332, 544, 758, 788, 910, 1034
Hpy8I	GTNNAC	1 cut(s)	719
Hpy99I	CGWCG	1 cut(s)	284
HpyAV	CCTTC	3 cut(s)	274, 530, 541
HpyCH4III	ACNGT	6 cut(s)	25, 83, 284, 317, 345, 1105
HpyCH4IV	ACGT	2 cut(s)	721, 841
HpyCH4V	TGCA	4 cut(s)	105, 927, 981, 1100
HpyF10VI	GCNNNNNNNGC	2 cut(s)	41, 476
HpyF3I	CTNAG	4 cut(s)	5, 348, 786, 889
HpySE526I	ACGT	2 cut(s)	721, 841
Hsp92I	GRCGYC	1 cut(s)	1005
Hsp92II	CATG	3 cut(s)	144, 641, 931
HspAI	GCGC	1 cut(s)	218
Kzo9I	GATC	3 cut(s)	72, 673, 830
LmnI	GCTCC	3 cut(s)	550, 634, 653
Lsp1109I	GCAGC	7 cut(s)	19, 99, 232, 457, 460, 463, 1084
LweI	GCATC	3 cut(s)	751, 772, 915
MaeI	CTAG	1 cut(s)	393
MaeII	ACGT	2 cut(s)	721, 841
MaeIII	GTNAC	4 cut(s)	370, 766, 915, 1006
MalI	GATC	3 cut(s)	74, 675, 832
MboI	GATC	3 cut(s)	72, 673, 830
MboII	GAAGA	5 cut(s)	58, 262, 602, 613, 980
MluCI	AATT	5 cut(s)	450, 496, 679, 696, 899
MlyI	GAGTC	3 cut(s)	188, 245, 1039
MmeI	TCCRAC	2 cut(s)	631, 646
MnlI	CCTC	8 cut(s)	184, 223, 234, 254, 436, 439, 526, 877
MseI	TTAA	1 cut(s)	495
MspI	CCGG	1 cut(s)	208
MspR9I	CCNGG	4 cut(s)	208, 209, 621, 732
Mva1269I	GAATGC	1 cut(s)	238
MvaI	CCWGG	2 cut(s)	621, 732
MvnI	CGCG	1 cut(s)	218
MwoI	GCNNNNNNNGC	2 cut(s)	41, 476
NciI	CCSGG	2 cut(s)	208, 209
NdeII	GATC	3 cut(s)	72, 673, 830
NlaIII	CATG	3 cut(s)	144, 641, 931
NmuCI	GTSAC	3 cut(s)	766, 915, 1006
PaqCI	CACCTGC	1 cut(s)	788
PctI	GAATGC	1 cut(s)	238
PflFI	GACNNNGTC	1 cut(s)	317
PflMI	CCANNNNNTGG	2 cut(s)	331, 593
PkrI	GCNGC	8 cut(s)	34, 37, 89, 222, 472, 475, 478, 1099
PleI	GAGTC	3 cut(s)	187, 245, 1038
PpsI	GAGTC	3 cut(s)	187, 245, 1038
Ppu21I	YACGTR	1 cut(s)	722
PshBI	ATTAAT	1 cut(s)	495
Psp6I	CCWGG	2 cut(s)	619, 730
PspGI	CCWGG	2 cut(s)	619, 730
PspPI	GGNCC	2 cut(s)	210, 584
PsyI	GACNNNGTC	1 cut(s)	317
RsaI	GTAC	4 cut(s)	112, 127, 575, 720
RsaNI	GTAC	4 cut(s)	111, 126, 574, 719
SaqAI	TTAA	1 cut(s)	495
SatI	GCNGC	8 cut(s)	33, 36, 88, 221, 471, 474, 477, 1098
Sau3AI	GATC	3 cut(s)	72, 673, 830
Sau96I	GGNCC	2 cut(s)	210, 584
SchI	GAGTC	3 cut(s)	188, 245, 1039
ScrFI	CCNGG	4 cut(s)	208, 209, 621, 732
SfaNI	GCATC	3 cut(s)	751, 772, 915
SfcI	CTRYAG	1 cut(s)	255
SmaI	CCCGGG	1 cut(s)	209
SmlI	CTYRAG	2 cut(s)	554, 1091
SmoI	CTYRAG	2 cut(s)	554, 1091
Sse9I	AATT	5 cut(s)	450, 496, 679, 696, 899
SsiI	CCGC	2 cut(s)	35, 216
SspMI	CTAG	1 cut(s)	393
StyD4I	CCNGG	4 cut(s)	206, 207, 619, 730
StyI	CCWWGG	1 cut(s)	577
TaaI	ACNGT	6 cut(s)	25, 83, 284, 317, 345, 1105
TaiI	ACGT	2 cut(s)	724, 844
TaqI	TCGA	2 cut(s)	273, 676
TasI	AATT	5 cut(s)	450, 496, 679, 696, 899
TauI	GCSGC	1 cut(s)	38
Tru1I	TTAA	1 cut(s)	495
Tru9I	TTAA	1 cut(s)	495
TscAI	CASTG	2 cut(s)	30, 1026
TseFI	GTSAC	3 cut(s)	766, 915, 1006
TseI	GCWGC	7 cut(s)	32, 87, 220, 470, 473, 476, 1097
Tsp45I	GTSAC	3 cut(s)	766, 915, 1006
TspDTI	ATGAA	4 cut(s)	139, 256, 733, 912
TspGWI	ACGGA	1 cut(s)	815
TspMI	CCCGGG	1 cut(s)	207
TspRI	CASTG	2 cut(s)	30, 1026
Tth111I	GACNNNGTC	1 cut(s)	317
Van91I	CCANNNNNTGG	2 cut(s)	331, 593
VspI	ATTAAT	1 cut(s)	495
XapI	RAATTY	4 cut(s)	450, 679, 696, 899
XmaI	CCCGGG	1 cut(s)	207
XspI	CTAG	1 cut(s)	393

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.