Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0006014

Physical Location & Seq

Genomic Coordinates Forward (+)

32883606 .. 32887594

Gene Structure 3989 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1262300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1128 bp

ATGTCAGCAACAACAGTGATAGCTTCTGCTTCTGCTTCCGCGAAGAGTTCCCAATTGTTTGGGTTTGGGTCCTTTGGGATCAACAGCAACAGCAGCAACAATCTCTCTTGCACTCGGTCTACTACTACTACTACTACTACTAGTACACGTAATCTCTCATGGAACTTCATCAACAGCAACAAGCACAAGTCTAGGAAGTCAATGATGGAGGACAGCTTCACCACTCGGGCCTCCGCGCAGCCTCTCACGAATGCTGATGACCTCATTGACTCTGTGGAGACTTTCATCTTCGACTGCGATGGTGTTATATGGAAAGGAGATAAGCTCATAGATGGTGTCCCCCAAACTCTGGACATGCTCCGGTCCAAGGGAAAAAGATTAGTTTTCGTCACCAACAACTCAACAAAGTCTAGGAAGCAGTACGGTAAAAAGTTTGAGACGCTTGGTCTGAATGTCAATGAGGAGGAAATTTTTGCATCGTCTTTTGCTGCCGCTGCCTATTTGAAGTCCATTAATTTCCCAAAAGATAAAAAGGTCTATGTGATTGGTGAGGAAGGCATCTTGAAGGAGCTTGAGCTTGCTGGATATCAGTACCTTGGTGGACCAGAAGATGGTGGGAAGAAAATAGAGCTGAAACCTGGATATCTGATGGAGCATGATGAGAGCGTTGGAGCTGTTGTGGTTGGATTTGATCGAAATTTCAACTACTACAAAATTCAGTATGGGACACTCTGTATACGTGAAAACCCTGGATGTCTTTTCATTGCTACGAATCGTGATGCTGTCACTCATCTTACAGATGCTCAGGAATGGGCAGGTGGTGGTTCAATGGTTGGTGCTATCAGTGGATCTACTCAACGCGAGCCATTGGTTGTGGGAAAACCCTCTACATTTATGATGGACTACTTAGCAAACGAATTTGGTATCCTGAAGTCACAGATCTGCATGGTTGGGGACAGGTTAGATACTGATATTCTGTTTGGACAAAATGGTGGTTGCAAAACTCTTCTGGTTCTCTCAGGCGTCACCACCTTGCCGGTGCTTCAGAGTCCCGACAACTCCATACAACCAGATTTTTACACAAACAAGATTTCAGACTTTTTGTCTCTCAAGGCTGCAACTGTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006793 GO:0006796 GO:0008150 GO:0008152 GO:0009987 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0042578 GO:0044237 GO:0044424 GO:0044444 GO:0044464

KEGG Pathways

Metabolic & Signaling

ko00630 ko01100 ko01110 ko01130 ko01200 map00630 map01100 map01110 map01130 map01200

Pfam Domains

Protein Families

Hydrolase_6 Hydrolase_like

Protein Analysis

376

Amino Acids

40.87

Weight (kDa)

6.03

Isoelectric Point (pI)

30.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Hydrolase	PF00702	93 - 332	7.7e-14	haloacid dehalogenase-like hydrolase
Hydrolase_6	PF13344	95 - 197	1e-34	Haloacid dehalogenase-like hydrolase
Hydrolase_like	PF13242	292 - 366	1.9e-20	HAD-hyrolase-like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0006014)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36700 AT5G36700 AT5G36700 AT5G36700 AT5G36790 AT5G36790 AT5G36790
fragaria_vesca	FvH4_6g00110 FvH4_6g00110
malus_domestica	MD04G1248400.v1.1 MD12G1262300.v1.1
prunus_persica	Prupe.6G364700_v2.0.a1 Prupe.6G364700_v2.0.a1 Prupe.6G364700_v2.0.a1 Prupe.6G364700_v2.0.a1
pyrus_communis	pycom04g21910 pycom12g24170
rosa_chinensis	RchiOBHm_Chr3g0447461
rosa_laevigata	RLG00000025996
rosa_roxburghii	Rroxscaffold_6G00425680
rosa_rugosa	Rorug02G0601900 Rorug02G0602000
rosa_samantha	Rh3AG002200 Rh3BG001700 Rh3CG002000 Rh3DG002100
rosa_wichuraiana	Rw3G000110

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	806
Acc36I	ACCTGC	1 cut(s)	806
AccB7I	CCANNNNNTGG	2 cut(s)	349, 611
AccI	GTMKAC	2 cut(s)	119, 736
AccII	CGCG	3 cut(s)	41, 236, 861
AciI	CCGC	3 cut(s)	39, 234, 492
AclWI	GGATC	2 cut(s)	86, 856
AcsI	RAATTY	4 cut(s)	468, 697, 714, 917
AcuI	CTGAAG	2 cut(s)	950, 1028
AcyI	GRCGYC	1 cut(s)	1023
AfaI	GTAC	3 cut(s)	145, 422, 593
AfiI	CCNNNNNNNGG	3 cut(s)	349, 367, 611
AflIII	ACRYGT	1 cut(s)	146
AgsI	TTSAA	4 cut(s)	505, 565, 703, 828
AhdI	GACNNNNNGTC	2 cut(s)	444, 1102
AhlI	ACTAGT	1 cut(s)	140
AjnI	CCWGG	2 cut(s)	637, 748
AluBI	AGCT	7 cut(s)	23, 216, 325, 571, 577, 631, 674
AluI	AGCT	7 cut(s)	23, 216, 325, 571, 577, 631, 674
Alw26I	GTCTC	3 cut(s)	272, 431, 1110
AlwI	GGATC	2 cut(s)	86, 856
Ama87I	CYCGRG	1 cut(s)	225
AoxI	GGCC	1 cut(s)	228
ApeKI	GCWGC	5 cut(s)	93, 238, 488, 494, 1115
ApoI	RAATTY	4 cut(s)	468, 697, 714, 917
AseI	ATTAAT	1 cut(s)	513
AspLEI	GCGC	1 cut(s)	238
AspS9I	GGNCC	4 cut(s)	69, 228, 363, 602
AsuHPI	GGTGA	4 cut(s)	211, 382, 560, 1018
AvaI	CYCGRG	1 cut(s)	225
AvaII	GGWCC	3 cut(s)	69, 363, 602
BbvI	GCAGC	5 cut(s)	105, 250, 475, 481, 1102
BccI	CCATC	6 cut(s)	199, 293, 326, 605, 643, 892
BciT130I	CCWGG	2 cut(s)	639, 750
BciVI	GTATCC	1 cut(s)	935
BcoDI	GTCTC	3 cut(s)	272, 431, 1110
BcuI	ACTAGT	1 cut(s)	140
BfaI	CTAG	3 cut(s)	141, 192, 411
BfuAI	ACCTGC	1 cut(s)	806
BfuI	GTATCC	1 cut(s)	935
BglII	AGATCT	1 cut(s)	939
BisI	GCNGC	6 cut(s)	94, 239, 489, 492, 495, 1116
BlsI	GCNGC	6 cut(s)	95, 240, 490, 493, 496, 1117
Bme1390I	CCNGG	2 cut(s)	639, 750
Bme18I	GGWCC	3 cut(s)	69, 363, 602
BmeRI	GACNNNNNGTC	2 cut(s)	444, 1102
BmeT110I	CYCGRG	1 cut(s)	225
BmgT120I	GGNCC	4 cut(s)	69, 228, 363, 602
BmiI	GGNNCC	1 cut(s)	70
BmrFI	CCNGG	2 cut(s)	639, 750
BmsI	GCATC	4 cut(s)	485, 567, 769, 790
BplI	GAGNNNNNCTC	2 cut(s)	309, 341
Bpu10I	CCTNAGC	1 cut(s)	804
BpuEI	CTTGAG	2 cut(s)	593, 1094
BsaAI	YACGTR	2 cut(s)	149, 740
BsaHI	GRCGYC	1 cut(s)	1023
BsaJI	CCNNGG	3 cut(s)	366, 595, 748
BsaWI	WCCGGW	1 cut(s)	360
BsaXI	ACNNNNNCTCC	2 cut(s)	663, 693
Bsc4I	CCNNNNNNNGG	3 cut(s)	349, 367, 611
Bse118I	RCCGGY	1 cut(s)	1036
Bse3DI	GCAATG	1 cut(s)	762
BseBI	CCWGG	2 cut(s)	639, 750
BseDI	CCNNGG	3 cut(s)	366, 595, 748
BseGI	GGATG	1 cut(s)	758
BseLI	CCNNNNNNNGG	3 cut(s)	349, 367, 611
BseMI	GCAATG	1 cut(s)	762
BseMII	CTCAG	2 cut(s)	818, 1032
BseRI	GAGGAG	1 cut(s)	476
BseXI	GCAGC	5 cut(s)	105, 250, 475, 481, 1102
Bsh1236I	CGCG	3 cut(s)	41, 236, 861
BshFI	GGCC	1 cut(s)	230
BsiHKCI	CYCGRG	1 cut(s)	225
BsiSI	CCGG	2 cut(s)	361, 1037
BslFI	GGGAC	4 cut(s)	323, 739, 968, 1035
BslI	CCNNNNNNNGG	3 cut(s)	349, 367, 611
BsmAI	GTCTC	3 cut(s)	272, 431, 1110
BsmBI	CGTCTC	1 cut(s)	431
BsmFI	GGGAC	4 cut(s)	323, 739, 968, 1035
BsmI	GAATGC	1 cut(s)	256
BsnI	GGCC	1 cut(s)	230
BsoBI	CYCGRG	1 cut(s)	225
Bsp143I	GATC	4 cut(s)	78, 691, 848, 939
BspACI	CCGC	3 cut(s)	39, 234, 492
BspANI	GGCC	1 cut(s)	230
BspCNI	CTCAG	2 cut(s)	817, 1031
BspFNI	CGCG	3 cut(s)	41, 236, 861
BspLI	GGNNCC	1 cut(s)	70
BspMI	ACCTGC	1 cut(s)	806
BspPI	GGATC	2 cut(s)	86, 856
BsrDI	GCAATG	1 cut(s)	762
BsrFI	RCCGGY	1 cut(s)	1036
BssAI	RCCGGY	1 cut(s)	1036
BssECI	CCNNGG	3 cut(s)	366, 595, 748
BssMI	GATC	4 cut(s)	78, 691, 848, 939
BssNAI	GTATAC	1 cut(s)	737
BssNI	GRCGYC	1 cut(s)	1023
BssT1I	CCWWGG	2 cut(s)	366, 595
Bst1107I	GTATAC	1 cut(s)	737
Bst2UI	CCWGG	2 cut(s)	639, 750
Bst4CI	ACNGT	3 cut(s)	16, 425, 1123
Bst6I	CTCTTC	2 cut(s)	38, 1011
BstACI	GRCGYC	1 cut(s)	1023
BstBAI	YACGTR	2 cut(s)	149, 740
BstC8I	GCNNGC	2 cut(s)	579, 863
BstDEI	CTNAG	3 cut(s)	804, 907, 1018
BstF5I	GGATG	1 cut(s)	758
BstFNI	CGCG	3 cut(s)	41, 236, 861
BstHHI	GCGC	1 cut(s)	238
BstKTI	GATC	4 cut(s)	81, 694, 851, 942
BstMAI	GTCTC	3 cut(s)	272, 431, 1110
BstMBI	GATC	4 cut(s)	78, 691, 848, 939
BstMWI	GCNNNNNNNGC	2 cut(s)	93, 494
BstNI	CCWGG	2 cut(s)	639, 750
BstNSI	RCATGY	1 cut(s)	358
BstSCI	CCNGG	2 cut(s)	637, 748
BstUI	CGCG	3 cut(s)	41, 236, 861
BstV1I	GCAGC	5 cut(s)	105, 250, 475, 481, 1102
BstX2I	RGATCY	2 cut(s)	848, 939
BstXI	CCANNNNNNTGG	1 cut(s)	59
BstYI	RGATCY	2 cut(s)	848, 939
BstZ17I	GTATAC	1 cut(s)	737
BsuI	GTATCC	1 cut(s)	935
BsuRI	GGCC	1 cut(s)	230
BtgZI	GCGATG	1 cut(s)	312
BtsCI	GGATG	1 cut(s)	758
BtsIMutI	CAGTG	2 cut(s)	21, 850
BveI	ACCTGC	1 cut(s)	806
Cac8I	GCNNGC	2 cut(s)	579, 863
CfoI	GCGC	1 cut(s)	238
Cfr10I	RCCGGY	1 cut(s)	1036
Cfr13I	GGNCC	4 cut(s)	69, 228, 363, 602
CseI	GACGC	2 cut(s)	448, 1012
Csp6I	GTAC	3 cut(s)	144, 421, 592
CviAII	CATG	4 cut(s)	159, 355, 656, 946
CviQI	GTAC	3 cut(s)	144, 421, 592
DdeI	CTNAG	3 cut(s)	804, 907, 1018
DpnI	GATC	4 cut(s)	80, 693, 850, 941
DpnII	GATC	4 cut(s)	78, 691, 848, 939
DriI	GACNNNNNGTC	2 cut(s)	444, 1102
Eam1104I	CTCTTC	2 cut(s)	38, 1011
Eam1105I	GACNNNNNGTC	2 cut(s)	444, 1102
EarI	CTCTTC	2 cut(s)	38, 1011
Eco130I	CCWWGG	2 cut(s)	366, 595
Eco32I	GATATC	2 cut(s)	587, 644
Eco47I	GGWCC	3 cut(s)	69, 363, 602
Eco57I	CTGAAG	2 cut(s)	950, 1028
Eco88I	CYCGRG	1 cut(s)	225
EcoO109I	RGGNCCY	1 cut(s)	69
EcoRII	CCWGG	2 cut(s)	637, 748
EcoRV	GATATC	2 cut(s)	587, 644
EcoT14I	CCWWGG	2 cut(s)	366, 595
ErhI	CCWWGG	2 cut(s)	366, 595
Esp3I	CGTCTC	1 cut(s)	431
FaeI	CATG	4 cut(s)	162, 358, 659, 949
FaqI	GGGAC	4 cut(s)	323, 739, 968, 1035
FatI	CATG	4 cut(s)	158, 354, 655, 945
FblI	GTMKAC	2 cut(s)	119, 736
Fnu4HI	GCNGC	6 cut(s)	94, 239, 489, 492, 495, 1116
FokI	GGATG	1 cut(s)	765
Fsp4HI	GCNGC	6 cut(s)	94, 239, 489, 492, 495, 1116
FspBI	CTAG	3 cut(s)	141, 192, 411
GlaI	GCGC	1 cut(s)	237
GluI	GCNGC	6 cut(s)	94, 239, 489, 492, 495, 1116
HaeIII	GGCC	1 cut(s)	230
HapII	CCGG	2 cut(s)	361, 1037
HgaI	GACGC	2 cut(s)	448, 1012
HhaI	GCGC	1 cut(s)	238
Hin1I	GRCGYC	1 cut(s)	1023
Hin1II	CATG	4 cut(s)	162, 358, 659, 949
Hin6I	GCGC	1 cut(s)	236
HinP1I	GCGC	1 cut(s)	236
HinfI	GANTC	3 cut(s)	269, 772, 1048
HpaII	CCGG	2 cut(s)	361, 1037
HphI	GGTGA	4 cut(s)	211, 382, 560, 1018
Hpy166II	GTNNAC	4 cut(s)	120, 146, 602, 737
Hpy188I	TCNGA	4 cut(s)	450, 648, 1047, 1096
Hpy188III	TCNNGA	7 cut(s)	247, 350, 562, 776, 806, 928, 1052
Hpy8I	GTNNAC	4 cut(s)	120, 146, 602, 737
HpyAV	CCTTC	2 cut(s)	548, 559
HpyCH4III	ACNGT	3 cut(s)	16, 425, 1123
HpyCH4IV	ACGT	2 cut(s)	148, 739
HpyCH4V	TGCA	5 cut(s)	111, 476, 945, 999, 1118
HpyF10VI	GCNNNNNNNGC	2 cut(s)	93, 494
HpyF3I	CTNAG	3 cut(s)	804, 907, 1018
HpySE526I	ACGT	2 cut(s)	148, 739
Hsp92I	GRCGYC	1 cut(s)	1023
Hsp92II	CATG	4 cut(s)	162, 358, 659, 949
HspAI	GCGC	1 cut(s)	236
Kzo9I	GATC	4 cut(s)	78, 691, 848, 939
LmnI	GCTCC	4 cut(s)	363, 568, 652, 671
Lsp1109I	GCAGC	5 cut(s)	105, 250, 475, 481, 1102
LweI	GCATC	4 cut(s)	485, 567, 769, 790
MaeI	CTAG	3 cut(s)	141, 192, 411
MaeII	ACGT	2 cut(s)	148, 739
MaeIII	GTNAC	4 cut(s)	388, 784, 933, 1024
MalI	GATC	4 cut(s)	80, 693, 850, 941
MboI	GATC	4 cut(s)	78, 691, 848, 939
MboII	GAAGA	5 cut(s)	55, 280, 620, 631, 998
MfeI	CAATTG	1 cut(s)	53
MflI	RGATCY	2 cut(s)	848, 939
MluCI	AATT	6 cut(s)	53, 468, 514, 697, 714, 917
MlyI	GAGTC	2 cut(s)	263, 1057
MmeI	TCCRAC	2 cut(s)	649, 664
MnlI	CCTC	8 cut(s)	202, 241, 252, 272, 454, 457, 544, 895
MseI	TTAA	1 cut(s)	513
MspA1I	CMGCKG	1 cut(s)	494
MspI	CCGG	2 cut(s)	361, 1037
MspR9I	CCNGG	2 cut(s)	639, 750
MunI	CAATTG	1 cut(s)	53
Mva1269I	GAATGC	1 cut(s)	256
MvaI	CCWGG	2 cut(s)	639, 750
MvnI	CGCG	3 cut(s)	41, 236, 861
MwoI	GCNNNNNNNGC	2 cut(s)	93, 494
NdeII	GATC	4 cut(s)	78, 691, 848, 939
NlaIII	CATG	4 cut(s)	162, 358, 659, 949
NlaIV	GGNNCC	1 cut(s)	70
NmuCI	GTSAC	4 cut(s)	388, 784, 933, 1024
NspI	RCATGY	1 cut(s)	358
PaqCI	CACCTGC	1 cut(s)	806
PctI	GAATGC	1 cut(s)	256
PfeI	GAWTC	1 cut(s)	772
PflMI	CCANNNNNTGG	2 cut(s)	349, 611
PkrI	GCNGC	6 cut(s)	95, 240, 490, 493, 496, 1117
PleI	GAGTC	2 cut(s)	263, 1056
PpsI	GAGTC	2 cut(s)	263, 1056
Ppu21I	YACGTR	2 cut(s)	149, 740
PpuMI	RGGWCCY	1 cut(s)	69
PshBI	ATTAAT	1 cut(s)	513
Psp5II	RGGWCCY	1 cut(s)	69
Psp6I	CCWGG	2 cut(s)	637, 748
PspGI	CCWGG	2 cut(s)	637, 748
PspN4I	GGNNCC	1 cut(s)	70
PspPI	GGNCC	4 cut(s)	69, 228, 363, 602
PspPPI	RGGWCCY	1 cut(s)	69
PsuI	RGATCY	2 cut(s)	848, 939
RsaI	GTAC	3 cut(s)	145, 422, 593
RsaNI	GTAC	3 cut(s)	144, 421, 592
SaqAI	TTAA	1 cut(s)	513
SatI	GCNGC	6 cut(s)	94, 239, 489, 492, 495, 1116
Sau3AI	GATC	4 cut(s)	78, 691, 848, 939
Sau96I	GGNCC	4 cut(s)	69, 228, 363, 602
SchI	GAGTC	2 cut(s)	263, 1057
ScrFI	CCNGG	2 cut(s)	639, 750
SfaNI	GCATC	4 cut(s)	485, 567, 769, 790
SinI	GGWCC	3 cut(s)	69, 363, 602
SmlI	CTYRAG	2 cut(s)	572, 1109
SmoI	CTYRAG	2 cut(s)	572, 1109
SpeI	ACTAGT	1 cut(s)	140
Sse9I	AATT	6 cut(s)	53, 468, 514, 697, 714, 917
SsiI	CCGC	3 cut(s)	39, 234, 492
SspMI	CTAG	3 cut(s)	141, 192, 411
StyD4I	CCNGG	2 cut(s)	637, 748
StyI	CCWWGG	2 cut(s)	366, 595
TaaI	ACNGT	3 cut(s)	16, 425, 1123
TaiI	ACGT	2 cut(s)	151, 742
TaqI	TCGA	2 cut(s)	291, 694
TaqII	GACCGA	1 cut(s)	105
TasI	AATT	6 cut(s)	53, 468, 514, 697, 714, 917
TatI	WGTACW	1 cut(s)	143
TauI	GCSGC	1 cut(s)	494
TfiI	GAWTC	1 cut(s)	772
Tru1I	TTAA	1 cut(s)	513
Tru9I	TTAA	1 cut(s)	513
TscAI	CASTG	2 cut(s)	21, 850
TseFI	GTSAC	4 cut(s)	388, 784, 933, 1024
TseI	GCWGC	5 cut(s)	93, 238, 488, 494, 1115
Tsp45I	GTSAC	4 cut(s)	388, 784, 933, 1024
TspDTI	ATGAA	3 cut(s)	157, 274, 751
TspRI	CASTG	2 cut(s)	21, 850
Van91I	CCANNNNNTGG	2 cut(s)	349, 611
VpaK11BI	GGWCC	3 cut(s)	69, 363, 602
VspI	ATTAAT	1 cut(s)	513
XapI	RAATTY	4 cut(s)	468, 697, 714, 917
XceI	RCATGY	1 cut(s)	358
XmiI	GTMKAC	2 cut(s)	119, 736
XspI	CTAG	3 cut(s)	141, 192, 411

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.