MD05G1000700.v1.1

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0007154
Physical Location & Seq
Reverse (-)
185184 .. 189567
4384 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1000700.v1.1.491

Sequence Viewer

Length: 906 bp
ATGACTCCAGCTAATCTGGCAGGGCAGTTTGGCGACACAACTTACACCAAGGTGTTTGTTGGAGGGTTGGCATGGGAAACCCAGAAGGAAACCATGAAGAAATACTTTGAACAGTTTGGAGACATCTTGGAGGCCGTTGTTATCACTGACAAGGCCACTGGAAGATCTAAAGGCTACGGATTTGTAACTTTTCGGGAAGCCGAAGCTGCGATGAGAGCTTGTGTGGATGCTGCTCCCGTGATTGATGGGAGGAGGGCCAACTGCAATCTTGCTTCTCTCGGTGTTCAGAGATCCAAGCCTTCAACTCCAAAGCATGGTGGAGGAGGAGGAGGAGGCAGGAGCTTTGGGAGGGTAATGGGGTCATTCCGAACAGGGTTTGGAGGTGGAGTGGGATCAGCTTTTCCCTCTGCAGCAAGCTTCCCTCATTATGCCATCCAACAAGGGATACCTTACAATCTTTATGGGTACTCTTCATACTCGCCAGACTACACTTACCCTACGAACTACTATGGTTTGTACGGAGGGGCAGCAGGCCAGTATCCTATGTACGGGACAAGCCCTGCAACTGGGATGGTGGCCGGAGCAGCAGCCGCGGCGGCTTTTTACCCTTATCTTTCATATGGATCAGAAGGGAATGGAGGAGCCGCAGCAGCTGCATATGGTAGCTCAGGTGTGCCGAGTTATGCAACTGGTGTCCAATACCCCCACAACAACCAGCAGCAGCAGCTTTTTCATTATTCGACCATGAATTCAAGTGGAGTTGGAGGCTACCAGCACTATGGTCCTCCCATTTCTCTTGCGCCCTCACCCGCCTTGCAATCACCAGACGCTAATATCCTTGACAGTGTGTTTTGCTGTGCCACAGGCGTAACCGTGGCCCTTCCTGCACCAATCCCTCATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006974 GO:0006977 GO:0006978 GO:0007049 GO:0007050 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008285 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010468 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0023052 GO:0030330 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031570 GO:0031571 GO:0033554 GO:0035556 GO:0042127 GO:0042770 GO:0042772 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0043487 GO:0043488 GO:0043489 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0045934 GO:0048255 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070935 GO:0071156 GO:0071158 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0097159 GO:1901363 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902369 GO:1902373 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903311 GO:1903312 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

302

Amino Acids

31.32

Weight (kDa)

8.53

Isoelectric Point (pI)

47.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 18 - 73 9.4e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 314
AccII CGCG 1 cut(s) 593
AciI CCGC 5 cut(s) 591, 593, 596, 645, 810
AclWI GGATC 3 cut(s) 285, 400, 631
AcoI YGGCCR 1 cut(s) 576
AcsI RAATTY 1 cut(s) 748
AfaI GTAC 3 cut(s) 467, 518, 548
AfiI CCNNNNNNNGG 3 cut(s) 314, 548, 566
AgsI TTSAA 3 cut(s) 110, 303, 753
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 9 cut(s) 11, 206, 218, 342, 398, 417, 653, 666, 727
AluI AGCT 9 cut(s) 11, 206, 218, 342, 398, 417, 653, 666, 727
Alw26I GTCTC 1 cut(s) 114
AlwI GGATC 3 cut(s) 285, 400, 631
AlwNI CAGNNNCTG 1 cut(s) 653
AoxI GGCC 6 cut(s) 132, 153, 255, 532, 576, 876
ApoI RAATTY 1 cut(s) 748
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 255, 782, 877
AsuHPI GGTGA 2 cut(s) 798, 813
AvaII GGWCC 1 cut(s) 782
BccI CCATC 3 cut(s) 239, 440, 565
BceAI ACGGC 1 cut(s) 119
BciVI GTATCC 2 cut(s) 438, 549
BcoDI GTCTC 1 cut(s) 114
BfaI CTAG 1 cut(s) 904
BfmI CTRYAG 1 cut(s) 408
BfuI GTATCC 2 cut(s) 438, 549
BglI GCCNNNNNGGC 1 cut(s) 596
BglII AGATCT 1 cut(s) 164
Bme18I GGWCC 1 cut(s) 782
BmgT120I GGNCC 3 cut(s) 255, 782, 877
BmiI GGNNCC 1 cut(s) 643
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 1 cut(s) 217
BmuI ACTGGG 1 cut(s) 576
Bpu10I CCTNAGC 1 cut(s) 667
BsaJI CCNNGG 3 cut(s) 48, 591, 873
BsaXI ACNNNNNCTCC 2 cut(s) 372, 402
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 548, 566
Bse1I ACTGG 4 cut(s) 163, 535, 571, 694
BseDI CCNNGG 3 cut(s) 48, 591, 873
BseGI GGATG 3 cut(s) 232, 432, 576
BseLI CCNNNNNNNGG 3 cut(s) 314, 548, 566
BseMII CTCAG 1 cut(s) 681
BseNI ACTGG 4 cut(s) 163, 535, 571, 694
BseRI GAGGAG 6 cut(s) 265, 336, 339, 342, 345, 654
BsgI GTGCAG 1 cut(s) 870
Bsh1236I CGCG 1 cut(s) 593
BshFI GGCC 6 cut(s) 134, 155, 257, 534, 578, 878
BsiSI CCGG 1 cut(s) 579
BslFI GGGAC 1 cut(s) 565
BslI CCNNNNNNNGG 3 cut(s) 314, 548, 566
BsmAI GTCTC 1 cut(s) 114
BsmFI GGGAC 1 cut(s) 565
BsnI GGCC 6 cut(s) 134, 155, 257, 534, 578, 878
Bsp143I GATC 4 cut(s) 164, 290, 392, 623
BspACI CCGC 5 cut(s) 591, 593, 596, 645, 810
BspANI GGCC 6 cut(s) 134, 155, 257, 534, 578, 878
BspCNI CTCAG 1 cut(s) 680
BspFNI CGCG 1 cut(s) 593
BspLI GGNNCC 1 cut(s) 643
BspMAI CTGCAG 1 cut(s) 412
BspPI GGATC 3 cut(s) 285, 400, 631
BsrI ACTGG 4 cut(s) 163, 535, 571, 694
BssECI CCNNGG 3 cut(s) 48, 591, 873
BssMI GATC 4 cut(s) 164, 290, 392, 623
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 114, 845, 874
Bst6I CTCTTC 1 cut(s) 475
BstAPI GCANNNNNTGC 1 cut(s) 653
BstC8I GCNNGC 2 cut(s) 415, 532
BstDEI CTNAG 1 cut(s) 667
BstDSI CCRYGG 2 cut(s) 591, 873
BstF5I GGATG 3 cut(s) 232, 432, 576
BstFNI CGCG 1 cut(s) 593
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 4 cut(s) 167, 293, 395, 626
BstMAI GTCTC 1 cut(s) 114
BstMBI GATC 4 cut(s) 164, 290, 392, 623
BstSFI CTRYAG 1 cut(s) 408
BstUI CGCG 1 cut(s) 593
BstX2I RGATCY 2 cut(s) 164, 290
BstXI CCANNNNNNTGG 1 cut(s) 779
BstYI RGATCY 2 cut(s) 164, 290
BsuI GTATCC 2 cut(s) 438, 549
BsuRI GGCC 6 cut(s) 134, 155, 257, 534, 578, 878
BtgI CCRYGG 2 cut(s) 591, 873
BtgZI GCGATG 2 cut(s) 224, 884
BtsCI GGATG 3 cut(s) 232, 432, 576
BtsIMutI CAGTG 3 cut(s) 144, 156, 850
Cac8I GCNNGC 2 cut(s) 415, 532
CaiI CAGNNNCTG 1 cut(s) 653
CfoI GCGC 1 cut(s) 802
Cfr13I GGNCC 3 cut(s) 255, 782, 877
Cfr42I CCGCGG 1 cut(s) 594
CseI GACGC 1 cut(s) 836
Csp6I GTAC 3 cut(s) 466, 517, 547
CviAII CATG 4 cut(s) 72, 94, 314, 745
CviQI GTAC 3 cut(s) 466, 517, 547
DdeI CTNAG 1 cut(s) 667
DpnI GATC 4 cut(s) 166, 292, 394, 625
DpnII GATC 4 cut(s) 164, 290, 392, 623
EaeI YGGCCR 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 475
EarI CTCTTC 1 cut(s) 475
Eco130I CCWWGG 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 782
EcoRI GAATTC 1 cut(s) 748
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 4 cut(s) 75, 97, 317, 748
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 565
FatI CATG 4 cut(s) 71, 93, 313, 744
FauI CCCGC 1 cut(s) 817
FauNDI CATATG 2 cut(s) 619, 658
FokI GGATG 3 cut(s) 239, 419, 583
FspBI CTAG 1 cut(s) 904
GlaI GCGC 1 cut(s) 801
HaeIII GGCC 6 cut(s) 134, 155, 257, 534, 578, 878
HapII CCGG 1 cut(s) 579
HgaI GACGC 1 cut(s) 836
HhaI GCGC 1 cut(s) 802
Hin1II CATG 4 cut(s) 75, 97, 317, 748
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HindIII AAGCTT 1 cut(s) 415
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 579
HphI GGTGA 2 cut(s) 798, 813
Hpy188I TCNGA 3 cut(s) 288, 368, 628
Hpy188III TCNNGA 1 cut(s) 194
HpyAV CCTTC 4 cut(s) 79, 309, 623, 890
HpyCH4III ACNGT 3 cut(s) 114, 845, 874
HpyCH4V TGCA 7 cut(s) 264, 410, 563, 656, 686, 817, 887
HpyF3I CTNAG 1 cut(s) 667
Hsp92II CATG 4 cut(s) 75, 97, 317, 748
HspAI GCGC 1 cut(s) 800
KspI CCGCGG 1 cut(s) 594
Kzo9I GATC 4 cut(s) 164, 290, 392, 623
LmnI GCTCC 4 cut(s) 238, 339, 581, 641
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 904
MaeIII GTNAC 2 cut(s) 184, 868
MalI GATC 4 cut(s) 166, 292, 394, 625
MboI GATC 4 cut(s) 164, 290, 392, 623
MboII GAAGA 3 cut(s) 109, 174, 462
MflI RGATCY 2 cut(s) 164, 290
MluCI AATT 1 cut(s) 748
MmeI TCCRAC 3 cut(s) 40, 460, 742
MslI CAYNNNNRTG 1 cut(s) 50
MspA1I CMGCKG 2 cut(s) 593, 653
MspI CCGG 1 cut(s) 579
MvnI CGCG 1 cut(s) 593
NdeI CATATG 2 cut(s) 619, 658
NdeII GATC 4 cut(s) 164, 290, 392, 623
NlaIII CATG 4 cut(s) 75, 97, 317, 748
NlaIV GGNNCC 1 cut(s) 643
NmeAIII GCCGAG 1 cut(s) 702
OliI CACNNNNGTG 1 cut(s) 50
PflMI CCANNNNNTGG 1 cut(s) 314
PspN4I GGNNCC 1 cut(s) 643
PspPI GGNCC 3 cut(s) 255, 782, 877
PstI CTGCAG 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 653
PsuI RGATCY 2 cut(s) 164, 290
PvuII CAGCTG 1 cut(s) 653
RsaI GTAC 3 cut(s) 467, 518, 548
RsaNI GTAC 3 cut(s) 466, 517, 547
RseI CAYNNNNRTG 1 cut(s) 50
SacII CCGCGG 1 cut(s) 594
Sau3AI GATC 4 cut(s) 164, 290, 392, 623
Sau96I GGNCC 3 cut(s) 255, 782, 877
SfaNI GCATC 1 cut(s) 217
SfcI CTRYAG 1 cut(s) 408
Sfr303I CCGCGG 1 cut(s) 594
SgrBI CCGCGG 1 cut(s) 594
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 1 cut(s) 50
Sse9I AATT 1 cut(s) 748
SsiI CCGC 5 cut(s) 591, 593, 596, 645, 810
SspMI CTAG 1 cut(s) 904
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 114, 845, 874
TaqI TCGA 1 cut(s) 740
TasI AATT 1 cut(s) 748
TauI GCSGC 4 cut(s) 593, 596, 599, 647
TscAI CASTG 3 cut(s) 151, 163, 850
TspDTI ATGAA 5 cut(s) 110, 462, 606, 722, 761
TspGWI ACGGA 2 cut(s) 192, 534
TspRI CASTG 3 cut(s) 151, 163, 850
Van91I CCANNNNNTGG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 1 cut(s) 748
XspI CTAG 1 cut(s) 904
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.