pycom215g00030

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000215
OG0007154
Physical Location & Seq
Forward (+)
135660 .. 136524
865 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom215g00030.1

Sequence Viewer

Length: 465 bp
ATGACTCCAGCTAATCTGGCAGGGCAGTTTGGCGACACGACTTACACCAAGGTGTTTGTTGGAGGGTTGGCATGGGAGACCCAGAAGGAAACCATGAAGAAATACTTTGAACAGTTTGGAGACATCTTGGAGGCCGTTGTTATCACTGACAAGGCCACTGGAAGATCTAAAGGCTACGGATTTGTAACTTTTCGGGAAGCCGAAGCTGCGATGAGAGCTTGTGTGGATGCTGCTCCCGTGATTGATGGGAGGAGGGCCAACTGCAATCTTGCTTCTCTCGGTGTTCAGAGATCCAAGCCTTCAACTCCGAAGCATGCGAACAGCCTTAAGAGTTTTGGATATTCCTCACTAATATCATTGGTGCTTTCCAAGAAGAGGTTATTGAATATATTAGATTTTGACTGTGGTCCGAAAATATTCTTATTTGAGGAAAATTCTTTTCACAGTGAAACATATATGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006974 GO:0006977 GO:0006978 GO:0007049 GO:0007050 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008285 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010468 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0023052 GO:0030330 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031570 GO:0031571 GO:0033554 GO:0035556 GO:0042127 GO:0042770 GO:0042772 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0043487 GO:0043488 GO:0043489 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0045934 GO:0048255 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070935 GO:0071156 GO:0071158 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0097159 GO:1901363 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902369 GO:1902373 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903311 GO:1903312 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

155

Amino Acids

17.04

Weight (kDa)

8.76

Isoelectric Point (pI)

32.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 18 - 73 2.7e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 285
AcsI RAATTY 1 cut(s) 433
AfiI CCNNNNNNNGG 1 cut(s) 375
AflII CTTAAG 1 cut(s) 326
AgsI TTSAA 3 cut(s) 110, 303, 385
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 3 cut(s) 11, 206, 218
AluI AGCT 3 cut(s) 11, 206, 218
Alw26I GTCTC 2 cut(s) 71, 114
AlwI GGATC 1 cut(s) 285
AoxI GGCC 3 cut(s) 132, 153, 255
ApeKI GCWGC 2 cut(s) 206, 230
ApoI RAATTY 1 cut(s) 433
Asp700I GAANNNNTTC 1 cut(s) 416
AspS9I GGNCC 2 cut(s) 255, 407
AvaII GGWCC 1 cut(s) 407
BbvI GCAGC 2 cut(s) 193, 217
BccI CCATC 1 cut(s) 239
BceAI ACGGC 1 cut(s) 119
BcoDI GTCTC 2 cut(s) 71, 114
BfrI CTTAAG 1 cut(s) 326
BglII AGATCT 1 cut(s) 164
BisI GCNGC 2 cut(s) 207, 231
BlsI GCNGC 2 cut(s) 208, 232
Bme18I GGWCC 1 cut(s) 407
BmgT120I GGNCC 2 cut(s) 255, 407
BmsI GCATC 1 cut(s) 217
BoxI GACNNNNGTC 1 cut(s) 405
BsaI GGTCTC 1 cut(s) 71
BsaJI CCNNGG 1 cut(s) 48
Bsc4I CCNNNNNNNGG 1 cut(s) 375
Bse1I ACTGG 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 1 cut(s) 232
BseLI CCNNNNNNNGG 1 cut(s) 375
BseNI ACTGG 1 cut(s) 163
BseRI GAGGAG 1 cut(s) 265
BseXI GCAGC 2 cut(s) 193, 217
BshFI GGCC 3 cut(s) 134, 155, 257
BslI CCNNNNNNNGG 1 cut(s) 375
BsmAI GTCTC 2 cut(s) 71, 114
BsnI GGCC 3 cut(s) 134, 155, 257
Bso31I GGTCTC 1 cut(s) 71
Bsp143I GATC 2 cut(s) 164, 290
BspANI GGCC 3 cut(s) 134, 155, 257
BspPI GGATC 1 cut(s) 285
BspTI CTTAAG 1 cut(s) 326
BspTNI GGTCTC 1 cut(s) 71
BsrI ACTGG 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 2 cut(s) 164, 290
BssT1I CCWWGG 1 cut(s) 48
Bst4CI ACNGT 3 cut(s) 114, 404, 446
Bst6I CTCTTC 1 cut(s) 368
BstAFI CTTAAG 1 cut(s) 326
BstC8I GCNNGC 1 cut(s) 315
BstF5I GGATG 1 cut(s) 232
BstKTI GATC 2 cut(s) 167, 293
BstMAI GTCTC 2 cut(s) 71, 114
BstMBI GATC 2 cut(s) 164, 290
BstMWI GCNNNNNNNGC 3 cut(s) 17, 206, 215
BstNSI RCATGY 1 cut(s) 317
BstPAI GACNNNNGTC 1 cut(s) 405
BstV1I GCAGC 2 cut(s) 193, 217
BstX2I RGATCY 2 cut(s) 164, 290
BstYI RGATCY 2 cut(s) 164, 290
BsuRI GGCC 3 cut(s) 134, 155, 257
BtgZI GCGATG 1 cut(s) 224
BtsCI GGATG 1 cut(s) 232
BtsIMutI CAGTG 3 cut(s) 144, 156, 451
Cac8I GCNNGC 1 cut(s) 315
Cfr13I GGNCC 2 cut(s) 255, 407
CviAII CATG 3 cut(s) 72, 94, 314
DpnI GATC 2 cut(s) 166, 292
DpnII GATC 2 cut(s) 164, 290
Eam1104I CTCTTC 1 cut(s) 368
EarI CTCTTC 1 cut(s) 368
Eco130I CCWWGG 1 cut(s) 48
Eco31I GGTCTC 1 cut(s) 71
Eco47I GGWCC 1 cut(s) 407
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 3 cut(s) 75, 97, 317
FaiI YATR 7 cut(s) 73, 95, 315, 389, 454, 456, 458
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FatI CATG 3 cut(s) 71, 93, 313
Fnu4HI GCNGC 2 cut(s) 207, 231
FokI GGATG 1 cut(s) 239
Fsp4HI GCNGC 2 cut(s) 207, 231
GluI GCNGC 2 cut(s) 207, 231
HaeIII GGCC 3 cut(s) 134, 155, 257
Hin1II CATG 3 cut(s) 75, 97, 317
HinfI GANTC 1 cut(s) 4
Hpy188I TCNGA 3 cut(s) 288, 309, 411
Hpy188III TCNNGA 1 cut(s) 194
HpyAV CCTTC 2 cut(s) 79, 309
HpyCH4III ACNGT 3 cut(s) 114, 404, 446
HpyCH4V TGCA 2 cut(s) 264, 460
HpyF10VI GCNNNNNNNGC 3 cut(s) 17, 206, 215
Hsp92II CATG 3 cut(s) 75, 97, 317
Kzo9I GATC 2 cut(s) 164, 290
LmnI GCTCC 1 cut(s) 238
LpnPI CCDG 5 cut(s) 2, 6, 21, 95, 144
Lsp1109I GCAGC 2 cut(s) 193, 217
LweI GCATC 1 cut(s) 217
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 166, 292
MboI GATC 2 cut(s) 164, 290
MboII GAAGA 3 cut(s) 109, 174, 385
MflI RGATCY 2 cut(s) 164, 290
MluCI AATT 1 cut(s) 433
MmeI TCCRAC 1 cut(s) 40
MnlI CCTC 7 cut(s) 56, 124, 243, 246, 355, 369, 421
MroXI GAANNNNTTC 1 cut(s) 416
MseI TTAA 1 cut(s) 327
MslI CAYNNNNRTG 1 cut(s) 50
MspCI CTTAAG 1 cut(s) 326
MwoI GCNNNNNNNGC 3 cut(s) 17, 206, 215
NdeII GATC 2 cut(s) 164, 290
NlaIII CATG 3 cut(s) 75, 97, 317
NspI RCATGY 1 cut(s) 317
OliI CACNNNNGTG 1 cut(s) 50
PaeI GCATGC 1 cut(s) 317
PdmI GAANNNNTTC 1 cut(s) 416
PkrI GCNGC 2 cut(s) 208, 232
PshAI GACNNNNGTC 1 cut(s) 405
PspPI GGNCC 2 cut(s) 255, 407
PsuI RGATCY 2 cut(s) 164, 290
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 327
SatI GCNGC 2 cut(s) 207, 231
Sau3AI GATC 2 cut(s) 164, 290
Sau96I GGNCC 2 cut(s) 255, 407
SetI ASST 5 cut(s) 13, 54, 208, 220, 380
SfaNI GCATC 1 cut(s) 217
SinI GGWCC 1 cut(s) 407
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 326
SmoI CTYRAG 1 cut(s) 326
SphI GCATGC 1 cut(s) 317
Sse9I AATT 1 cut(s) 433
SspI AATATT 1 cut(s) 417
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 3 cut(s) 114, 404, 446
TasI AATT 1 cut(s) 433
Tru1I TTAA 1 cut(s) 327
Tru9I TTAA 1 cut(s) 327
TscAI CASTG 3 cut(s) 151, 163, 451
TseI GCWGC 2 cut(s) 206, 230
TspDTI ATGAA 1 cut(s) 110
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 3 cut(s) 151, 163, 451
Vha464I CTTAAG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 407
XapI RAATTY 1 cut(s) 433
XceI RCATGY 1 cut(s) 317
XmnI GAANNNNTTC 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.