pycom10g00080

RNA-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0007154
Physical Location & Seq
Reverse (-)
69033 .. 74618
5586 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g00080.1

Sequence Viewer

Length: 867 bp
ATGACTCCAGCTAATCTGGCAGGGCAGTTTGGCGACACGACTTACACCAAGGTGTTTGTTGGAGGGTTGGCATGGGAAACCCAGAAGGAAACCATGAAGAAATACTTTGAACAGTTTGGTGACATCTTGGAGGCCGTTGTTATCACTGATAAGGCCACTGGGAGATCTAAGGGCTACGGATTTGTAACTTTTCGCGAAGCCGAAGCTGCGATGAGAGCTTGTGTGGATGCTGCTCCCGTGATTGATGGGAGGAGGGCTAACTGCAACCTTGCTTCTCTCGGTGTTCAGAGATCCAAGCCTTCAACTCCAAAGCATGGTAGTGGAGGAGGGGGAGGCAGGAGCTTTGGGAGGGTGGGGCCATTCCAAACAGGGTTTGGAGGAGGAGTGGGATCAGCTTTTCCCTCAGCAGCAACCTTCGCTCATTATGCCATCCAACAAGGGATACCTTACAATCTTTATGGGTACTCTTCATACTCGCCAGACTACACTTATCCTGCGAACTACTATGGTTTGTATGGAGGGGCAGCAGGCCAGTATCCTATGTACGGGACAAGCCCTGCAACTGGGATGGTGGCCGGAGCAGCAGCGGCGGCGGCATTTTACCCCTATCTTTCATATGGATCAGAAGGGAATGGAGGAGCCGCAGCAGGTGCGTATGGTAGCTCAGGTGTGCCGAGCTATGCAACTGGTGTCCAATTCCCCCACCAGCAGCAGCAGCAGCAGCAGCTTTTTCAGTATTCGACCATGAACTCAAGTGGAGTTGGAGGCTACCAGCACTATGGTCCTCCCATTTCTCTTGCGCCCTCGCCCGCCTTGCAGTCACCAGGCGTAACCGTGGCCCTTCCTGCACCAATTCCTCATCGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000075 GO:0000077 GO:0000278 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006950 GO:0006974 GO:0006977 GO:0006978 GO:0007049 GO:0007050 GO:0007093 GO:0007154 GO:0007165 GO:0007346 GO:0008150 GO:0008285 GO:0009892 GO:0009893 GO:0009894 GO:0009895 GO:0009987 GO:0010468 GO:0010564 GO:0010604 GO:0010605 GO:0010608 GO:0010628 GO:0010948 GO:0019219 GO:0019222 GO:0022402 GO:0023052 GO:0030330 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0031570 GO:0031571 GO:0033554 GO:0035556 GO:0042127 GO:0042770 GO:0042772 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043484 GO:0043487 GO:0043488 GO:0043489 GO:0044424 GO:0044444 GO:0044464 GO:0044773 GO:0044774 GO:0044783 GO:0044819 GO:0045786 GO:0045787 GO:0045930 GO:0045934 GO:0048255 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051716 GO:0051726 GO:0060255 GO:0061013 GO:0065007 GO:0065008 GO:0070935 GO:0071156 GO:0071158 GO:0072331 GO:0072395 GO:0072401 GO:0072413 GO:0072422 GO:0072431 GO:0080090 GO:0090068 GO:0097159 GO:1901363 GO:1901987 GO:1901988 GO:1901990 GO:1901991 GO:1902369 GO:1902373 GO:1902400 GO:1902402 GO:1902403 GO:1902806 GO:1902807 GO:1903047 GO:1903311 GO:1903312 GO:2000045 GO:2000134
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1

Protein Analysis

289

Amino Acids

29.92

Weight (kDa)

8.87

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 18 - 73 8.7e-16 RNA recognition motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 638
Acc36I ACCTGC 1 cut(s) 638
AccB7I CCANNNNNTGG 1 cut(s) 314
AccII CGCG 1 cut(s) 195
AciI CCGC 5 cut(s) 587, 590, 593, 642, 810
AclWI GGATC 3 cut(s) 285, 397, 628
AcoI YGGCCR 1 cut(s) 573
AfaI GTAC 2 cut(s) 464, 545
AfiI CCNNNNNNNGG 3 cut(s) 314, 545, 563
AgsI TTSAA 2 cut(s) 110, 303
AjnI CCWGG 1 cut(s) 823
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 8 cut(s) 11, 206, 218, 342, 395, 663, 678, 727
AluI AGCT 8 cut(s) 11, 206, 218, 342, 395, 663, 678, 727
AlwI GGATC 3 cut(s) 285, 397, 628
AoxI GGCC 6 cut(s) 132, 153, 356, 529, 573, 837
AspLEI GCGC 1 cut(s) 802
AspS9I GGNCC 3 cut(s) 356, 782, 838
AsuHPI GGTGA 2 cut(s) 131, 813
AvaII GGWCC 1 cut(s) 782
BbvCI CCTCAGC 1 cut(s) 403
BccI CCATC 3 cut(s) 239, 437, 562
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 825
BciVI GTATCC 2 cut(s) 435, 546
BfaI CTAG 1 cut(s) 865
BfuAI ACCTGC 1 cut(s) 638
BfuI GTATCC 2 cut(s) 435, 546
BglII AGATCT 1 cut(s) 164
Bme1390I CCNGG 1 cut(s) 825
Bme18I GGWCC 1 cut(s) 782
BmgT120I GGNCC 3 cut(s) 356, 782, 838
BmiI GGNNCC 2 cut(s) 357, 640
BmrFI CCNGG 1 cut(s) 825
BmrI ACTGGG 2 cut(s) 168, 573
BmsI GCATC 1 cut(s) 217
BmuI ACTGGG 2 cut(s) 168, 573
Bpu10I CCTNAGC 2 cut(s) 403, 664
BpuEI CTTGAG 1 cut(s) 736
BsaJI CCNNGG 2 cut(s) 48, 834
BsaXI ACNNNNNCTCC 2 cut(s) 369, 399
Bsc4I CCNNNNNNNGG 3 cut(s) 314, 545, 563
Bse1I ACTGG 4 cut(s) 163, 532, 568, 691
BseBI CCWGG 1 cut(s) 825
BseDI CCNNGG 2 cut(s) 48, 834
BseGI GGATG 3 cut(s) 232, 429, 573
BseLI CCNNNNNNNGG 3 cut(s) 314, 545, 563
BseMII CTCAG 2 cut(s) 417, 678
BseNI ACTGG 4 cut(s) 163, 532, 568, 691
BseRI GAGGAG 5 cut(s) 265, 339, 393, 396, 651
BsgI GTGCAG 1 cut(s) 831
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 6 cut(s) 134, 155, 358, 531, 575, 839
BsiSI CCGG 1 cut(s) 576
BslFI GGGAC 1 cut(s) 562
BslI CCNNNNNNNGG 3 cut(s) 314, 545, 563
BsmFI GGGAC 1 cut(s) 562
BsnI GGCC 6 cut(s) 134, 155, 358, 531, 575, 839
Bsp143I GATC 4 cut(s) 164, 290, 389, 620
Bsp68I TCGCGA 1 cut(s) 195
BspACI CCGC 5 cut(s) 587, 590, 593, 642, 810
BspANI GGCC 6 cut(s) 134, 155, 358, 531, 575, 839
BspCNI CTCAG 2 cut(s) 416, 677
BspFNI CGCG 1 cut(s) 195
BspLI GGNNCC 2 cut(s) 357, 640
BspMI ACCTGC 1 cut(s) 638
BspPI GGATC 3 cut(s) 285, 397, 628
BsrI ACTGG 4 cut(s) 163, 532, 568, 691
BssECI CCNNGG 2 cut(s) 48, 834
BssMI GATC 4 cut(s) 164, 290, 389, 620
BssT1I CCWWGG 1 cut(s) 48
Bst2UI CCWGG 1 cut(s) 825
Bst4CI ACNGT 2 cut(s) 114, 835
Bst6I CTCTTC 1 cut(s) 472
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 2 cut(s) 529, 810
BstDEI CTNAG 3 cut(s) 168, 403, 664
BstDSI CCRYGG 1 cut(s) 834
BstF5I GGATG 3 cut(s) 232, 429, 573
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 802
BstKTI GATC 4 cut(s) 167, 293, 392, 623
BstMBI GATC 4 cut(s) 164, 290, 389, 620
BstNI CCWGG 1 cut(s) 825
BstSCI CCNGG 1 cut(s) 823
BstUI CGCG 1 cut(s) 195
BstX2I RGATCY 2 cut(s) 164, 290
BstXI CCANNNNNNTGG 1 cut(s) 779
BstYI RGATCY 2 cut(s) 164, 290
BsuI GTATCC 2 cut(s) 435, 546
BsuRI GGCC 6 cut(s) 134, 155, 358, 531, 575, 839
BtgI CCRYGG 1 cut(s) 834
BtgZI GCGATG 2 cut(s) 224, 845
BtsCI GGATG 3 cut(s) 232, 429, 573
BtsIMutI CAGTG 2 cut(s) 144, 156
BtuMI TCGCGA 1 cut(s) 195
BveI ACCTGC 1 cut(s) 638
Cac8I GCNNGC 2 cut(s) 529, 810
CfoI GCGC 1 cut(s) 802
Cfr13I GGNCC 3 cut(s) 356, 782, 838
Csp6I GTAC 2 cut(s) 463, 544
CviAII CATG 4 cut(s) 72, 94, 314, 745
CviQI GTAC 2 cut(s) 463, 544
DdeI CTNAG 3 cut(s) 168, 403, 664
DpnI GATC 4 cut(s) 166, 292, 391, 622
DpnII GATC 4 cut(s) 164, 290, 389, 620
EaeI YGGCCR 1 cut(s) 573
Eam1104I CTCTTC 1 cut(s) 472
EarI CTCTTC 1 cut(s) 472
Eco130I CCWWGG 1 cut(s) 48
Eco47I GGWCC 1 cut(s) 782
EcoRII CCWGG 1 cut(s) 823
EcoT14I CCWWGG 1 cut(s) 48
ErhI CCWWGG 1 cut(s) 48
FaeI CATG 4 cut(s) 75, 97, 317, 748
FalI AAGNNNNNCTT 2 cut(s) 89, 121
FaqI GGGAC 1 cut(s) 562
FatI CATG 4 cut(s) 71, 93, 313, 744
FauI CCCGC 1 cut(s) 817
FauNDI CATATG 1 cut(s) 616
FokI GGATG 3 cut(s) 239, 416, 580
FspBI CTAG 1 cut(s) 865
GlaI GCGC 1 cut(s) 801
HaeIII GGCC 6 cut(s) 134, 155, 358, 531, 575, 839
HapII CCGG 1 cut(s) 576
HhaI GCGC 1 cut(s) 802
Hin1II CATG 4 cut(s) 75, 97, 317, 748
Hin6I GCGC 1 cut(s) 800
HinP1I GCGC 1 cut(s) 800
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 576
HphI GGTGA 2 cut(s) 131, 813
Hpy188I TCNGA 2 cut(s) 288, 625
Hpy188III TCNNGA 1 cut(s) 194
HpyAV CCTTC 5 cut(s) 79, 309, 424, 620, 851
HpyCH4III ACNGT 2 cut(s) 114, 835
HpyCH4V TGCA 5 cut(s) 264, 560, 683, 817, 848
HpyF3I CTNAG 3 cut(s) 168, 403, 664
Hsp92II CATG 4 cut(s) 75, 97, 317, 748
HspAI GCGC 1 cut(s) 800
Kzo9I GATC 4 cut(s) 164, 290, 389, 620
LmnI GCTCC 4 cut(s) 238, 339, 578, 638
LweI GCATC 1 cut(s) 217
MaeI CTAG 1 cut(s) 865
MaeIII GTNAC 4 cut(s) 119, 184, 819, 829
MalI GATC 4 cut(s) 166, 292, 391, 622
MboI GATC 4 cut(s) 164, 290, 389, 620
MboII GAAGA 2 cut(s) 109, 459
MflI RGATCY 2 cut(s) 164, 290
MluCI AATT 2 cut(s) 695, 852
MmeI TCCRAC 3 cut(s) 40, 457, 742
MslI CAYNNNNRTG 2 cut(s) 50, 318
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 1 cut(s) 576
MspR9I CCNGG 1 cut(s) 825
MvaI CCWGG 1 cut(s) 825
MvnI CGCG 1 cut(s) 195
NdeI CATATG 1 cut(s) 616
NdeII GATC 4 cut(s) 164, 290, 389, 620
NlaIII CATG 4 cut(s) 75, 97, 317, 748
NlaIV GGNNCC 2 cut(s) 357, 640
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 2 cut(s) 119, 819
NruI TCGCGA 1 cut(s) 195
OliI CACNNNNGTG 1 cut(s) 50
PaqCI CACCTGC 1 cut(s) 638
PflMI CCANNNNNTGG 1 cut(s) 314
Psp6I CCWGG 1 cut(s) 823
PspGI CCWGG 1 cut(s) 823
PspN4I GGNNCC 2 cut(s) 357, 640
PspPI GGNCC 3 cut(s) 356, 782, 838
PsuI RGATCY 2 cut(s) 164, 290
RruI TCGCGA 1 cut(s) 195
RsaI GTAC 2 cut(s) 464, 545
RsaNI GTAC 2 cut(s) 463, 544
RseI CAYNNNNRTG 2 cut(s) 50, 318
Sau3AI GATC 4 cut(s) 164, 290, 389, 620
Sau96I GGNCC 3 cut(s) 356, 782, 838
ScrFI CCNGG 1 cut(s) 825
SfaNI GCATC 1 cut(s) 217
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 2 cut(s) 50, 318
SmlI CTYRAG 1 cut(s) 751
SmoI CTYRAG 1 cut(s) 751
Sse9I AATT 2 cut(s) 695, 852
SsiI CCGC 5 cut(s) 587, 590, 593, 642, 810
SspMI CTAG 1 cut(s) 865
StyD4I CCNGG 1 cut(s) 823
StyI CCWWGG 1 cut(s) 48
TaaI ACNGT 2 cut(s) 114, 835
TaqI TCGA 1 cut(s) 740
TasI AATT 2 cut(s) 695, 852
TauI GCSGC 4 cut(s) 590, 593, 596, 644
TscAI CASTG 2 cut(s) 151, 163
TseFI GTSAC 2 cut(s) 119, 819
Tsp45I GTSAC 2 cut(s) 119, 819
TspDTI ATGAA 4 cut(s) 110, 459, 603, 761
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 2 cut(s) 151, 163
Van91I CCANNNNNTGG 1 cut(s) 314
VpaK11BI GGWCC 1 cut(s) 782
XcmI CCANNNNNNNNNTGG 1 cut(s) 371
XspI CTAG 1 cut(s) 865
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.