MD05G1026800.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010913
Physical Location & Seq
Reverse (-)
4276527 .. 4278144
1618 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1026800.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGGATCTAGAATTAGGACTCAAGATCACCCATACCGTAGAAGATCATGTCTCCTCTGCCAACTTTCAGATTGCCAAGGACAGATCCGGTCCTCTTTTCGTGTCTAAGGAAACCGAAAGCATGTTCATCCTCACCGGCCATCTTAAAGGTTATAGAAGAGAGCGTGTGTATATTGACATAAATGAAGATGGGACGCAGATTGGCATCAGTGGGGAGAAGCCAGTTCAAGAGAAGGTGATGTCAGGATGGATAATGTACAAGAAAGAGGTGGAGATAAGGGGTTTCAGAAAAGTCTTTCGAATTCCGGATGGAGTGGTTTTGGATCGAATCAAGGCCAAGTTTAATGTACACGAATCAAGTCTCACGATCGTCATGCCAAAATCAGTGAAAGGAATTCGCGGGGTTGGGATCGAGGAAGTGAAGGAAGATGAAGCGGATACAGGGAAACAGCAGCTAAAACAGCCTTCCGCAGCTGAAGAAGTCCCTGTAAAATATGGTTTTAGGGGAAAAATTGAGGGGGAAATTGGTGAGACCGAGAAAACGAAAGTAGAAGAAAATCGTCGAACTGATGCGAAAGGGGTTGATGAAGATGGCTCCAAGAAAGAAGTTGTAGCTGATGGAGTCATCGGAAAGGACACGAGAGGAAGCACAGTTAAGGAAGAAGCTAAAGATTCAAAGGCTCAAAGTATGCAACACAGAACAAAATCTGTTTCCGAAAACAATGAAGAGTCAAAAGTTGCAAAGGTAGAAGAAGCTGATGTAATTCTGAAAGAGAAAGTTGGAAGAAAGGAATTCGAAACAGATCAAACTGTAGCGAATAGAGTTACGAAGAGAGAAAATGCTGAAGTAACACGTGAAGTTAACAAGCCTTCACAAGAAAATCAGGTAACTGCTATGCATGACACAGCAAAAGAAAACTCTTCTGAGCTCCAAAAATTGGAAGCGCCTAAGGCAGGGATTGAGGAACACGTTCGAAGCAGACAAGAGAGCAGCGAATCATCAAAACAAACAGAAGATACAACAGCTACACAACAAGAAGCGCAAGTTCAACGCGAAAAGAATGCGGATGGAATTCGTAGAGAAGGGGATGCAGTTCAGCTACCAAAGCACGAATCCATCAAAGAAGGTCAGGGAAGCGGAGTTCAAGAAATAGCGAAGAACGAAGAACACATCAAAGAGAAGGATGAGGATGAATCTTCCGAAATGGAGGAACAAAGTGATGTGGATGATAAGAAATCAAGTGATGATCAAGAGAAGAAGGATGGTATGGAAAAGTCCAATCTGCTGTGCTCTCCTTTTATAATCGCAGGTTCAGCACTTATTGTGTCCCTGGTGATGCTAGCAATCAACAGGATTAGAACTAGAAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

458

Amino Acids

51.18

Weight (kDa)

5.44

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1301
Acc36I ACCTGC 1 cut(s) 1298
AccB7I CCANNNNNTGG 1 cut(s) 937
AccII CGCG 2 cut(s) 399, 1053
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 5 cut(s) 399, 434, 468, 1064, 1137
AclWI GGATC 4 cut(s) 12, 78, 330, 416
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 4 cut(s) 300, 393, 791, 1071
AcuI CTGAAG 2 cut(s) 495, 864
AcvI CACGTG 1 cut(s) 854
AfaI GTAC 2 cut(s) 257, 348
AfiI CCNNNNNNNGG 2 cut(s) 937, 953
AflIII ACRYGT 2 cut(s) 851, 967
AgsI TTSAA 4 cut(s) 227, 675, 1049, 1145
AjnI CCWGG 1 cut(s) 1329
AloI GAACNNNNNNTCC 2 cut(s) 1125, 1157
AluBI AGCT 8 cut(s) 454, 473, 614, 665, 755, 928, 1025, 1099
AluI AGCT 8 cut(s) 454, 473, 614, 665, 755, 928, 1025, 1099
Alw21I GWGCWC 2 cut(s) 930, 1292
Alw26I GTCTC 3 cut(s) 55, 365, 524
AlwI GGATC 4 cut(s) 12, 78, 330, 416
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 2 cut(s) 136, 333
ApeKI GCWGC 3 cut(s) 451, 470, 990
ApoI RAATTY 4 cut(s) 300, 393, 791, 1071
Asp700I GAANNNNTTC 1 cut(s) 969
AspLEI GCGC 2 cut(s) 946, 1042
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 19, 124, 247, 539, 1345
AsuII TTCGAA 3 cut(s) 298, 795, 973
AsuNHI GCTAGC 1 cut(s) 1339
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 1 cut(s) 948
BanII GRGCYC 1 cut(s) 930
BauI CACGAG 1 cut(s) 637
BbrPI CACGTG 1 cut(s) 854
Bbv12I GWGCWC 2 cut(s) 930, 1292
BbvI GCAGC 3 cut(s) 463, 482, 1002
BccI CCATC 9 cut(s) 147, 182, 240, 302, 584, 611, 1061, 1124, 1256
BcgI CGANNNNNNTGC 4 cut(s) 355, 389, 1043, 1077
BciT130I CCWGG 1 cut(s) 1331
BciVI GTATCC 1 cut(s) 430
BclI TGATCA 1 cut(s) 1246
BcoDI GTCTC 3 cut(s) 55, 365, 524
BfaI CTAG 3 cut(s) 8, 1340, 1362
BfmI CTRYAG 1 cut(s) 810
BfoI RGCGCY 1 cut(s) 947
BfuAI ACCTGC 1 cut(s) 1298
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 3 cut(s) 452, 471, 991
BlsI GCNGC 3 cut(s) 453, 472, 992
Bme1390I CCNGG 1 cut(s) 1331
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 1 cut(s) 595
BmrFI CCNGG 1 cut(s) 1331
BmsI GCATC 4 cut(s) 213, 559, 1078, 1326
BmtI GCTAGC 1 cut(s) 1343
Bpu14I TTCGAA 3 cut(s) 298, 795, 973
BpuEI CTTGAG 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 854
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 75, 1329
BsaWI WCCGGW 2 cut(s) 86, 304
Bsc4I CCNNNNNNNGG 2 cut(s) 937, 953
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 1 cut(s) 221
Bse21I CCTNAGG 1 cut(s) 948
Bse8I GATNNNNATC 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 1 cut(s) 1331
BseDI CCNNGG 2 cut(s) 75, 1329
BseGI GGATG 9 cut(s) 126, 251, 313, 1072, 1093, 1189, 1195, 1231, 1267
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 2 cut(s) 937, 953
BseMII CTCAG 1 cut(s) 915
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 43
BseXI GCAGC 3 cut(s) 463, 482, 1002
Bsh1236I CGCG 2 cut(s) 399, 1053
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 2 cut(s) 138, 335
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 2 cut(s) 930, 1292
BsiSI CCGG 3 cut(s) 87, 135, 305
BslFI GGGAC 3 cut(s) 205, 467, 1312
BslI CCNNNNNNNGG 2 cut(s) 937, 953
BsmAI GTCTC 3 cut(s) 55, 365, 524
BsmFI GGGAC 3 cut(s) 205, 467, 1312
BsmI GAATGC 1 cut(s) 1066
BsnI GGCC 2 cut(s) 138, 335
Bso31I GGTCTC 1 cut(s) 524
Bsp119I TTCGAA 3 cut(s) 298, 795, 973
Bsp1286I GDGCHC 2 cut(s) 930, 1292
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 2 cut(s) 255, 346
Bsp143I GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
BspACI CCGC 5 cut(s) 399, 434, 468, 1064, 1137
BspANI GGCC 2 cut(s) 138, 335
BspCNI CTCAG 1 cut(s) 916
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 2 cut(s) 399, 1053
BspLI GGNNCC 1 cut(s) 595
BspMI ACCTGC 1 cut(s) 1298
BspOI GCTAGC 1 cut(s) 1343
BspPI GGATC 4 cut(s) 12, 78, 330, 416
BspT104I TTCGAA 3 cut(s) 298, 795, 973
BspTNI GGTCTC 1 cut(s) 524
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 2 cut(s) 255, 346
BsrI ACTGG 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 2 cut(s) 75, 1329
BssMI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
BssSI CACGAG 1 cut(s) 637
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 637
Bst2UI CCWGG 1 cut(s) 1331
Bst4CI ACNGT 3 cut(s) 37, 652, 811
Bst6I CTCTTC 4 cut(s) 151, 720, 824, 925
BstAUI TGTACA 2 cut(s) 255, 346
BstBAI YACGTR 1 cut(s) 854
BstBI TTCGAA 3 cut(s) 298, 795, 973
BstC8I GCNNGC 1 cut(s) 1341
BstDEI CTNAG 3 cut(s) 105, 924, 948
BstENI CCTNNNNNAGG 1 cut(s) 951
BstF5I GGATG 9 cut(s) 126, 251, 313, 1072, 1093, 1189, 1195, 1231, 1267
BstFNI CGCG 2 cut(s) 399, 1053
BstH2I RGCGCY 1 cut(s) 947
BstHHI GCGC 2 cut(s) 946, 1042
BstKTI GATC 9 cut(s) 7, 27, 46, 86, 325, 369, 411, 805, 1249
BstMAI GTCTC 3 cut(s) 55, 365, 524
BstMBI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 460, 950, 1105, 1313
BstNI CCWGG 1 cut(s) 1331
BstNSI RCATGY 1 cut(s) 124
BstSCI CCNGG 1 cut(s) 1329
BstSFI CTRYAG 1 cut(s) 810
BstUI CGCG 2 cut(s) 399, 1053
BstV1I GCAGC 3 cut(s) 463, 482, 1002
BstX2I RGATCY 2 cut(s) 4, 83
BstYI RGATCY 2 cut(s) 4, 83
Bsu36I CCTNAGG 1 cut(s) 948
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 2 cut(s) 138, 335
BtsCI GGATG 9 cut(s) 126, 251, 313, 1072, 1093, 1189, 1195, 1231, 1267
BtsIMutI CAGTG 2 cut(s) 214, 390
BveI ACCTGC 1 cut(s) 1298
Cac8I GCNNGC 1 cut(s) 1341
CfoI GCGC 2 cut(s) 946, 1042
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 1 cut(s) 202
Csp6I GTAC 2 cut(s) 256, 347
CviAII CATG 4 cut(s) 47, 121, 373, 899
CviQI GTAC 2 cut(s) 256, 347
DdeI CTNAG 3 cut(s) 105, 924, 948
DpnI GATC 9 cut(s) 6, 26, 45, 85, 324, 368, 410, 804, 1248
DpnII GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 4 cut(s) 151, 720, 824, 925
EarI CTCTTC 4 cut(s) 151, 720, 824, 925
Ecl136II GAGCTC 1 cut(s) 928
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 930
Eco31I GGTCTC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 928
Eco57I CTGAAG 2 cut(s) 495, 864
Eco72I CACGTG 1 cut(s) 854
Eco81I CCTNAGG 1 cut(s) 948
EcoICRI GAGCTC 1 cut(s) 928
EcoNI CCTNNNNNAGG 1 cut(s) 951
EcoRI GAATTC 4 cut(s) 300, 393, 791, 1071
EcoRII CCWGG 1 cut(s) 1329
EcoT14I CCWWGG 1 cut(s) 75
EcoT22I ATGCAT 1 cut(s) 900
EcoT38I GRGCYC 1 cut(s) 930
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 50, 124, 376, 902
FaqI GGGAC 3 cut(s) 205, 467, 1312
FatI CATG 4 cut(s) 46, 120, 372, 898
FauI CCCGC 1 cut(s) 392
FbaI TGATCA 1 cut(s) 1246
Fnu4HI GCNGC 3 cut(s) 452, 471, 991
FokI GGATG 9 cut(s) 113, 258, 320, 1079, 1100, 1196, 1202, 1238, 1274
FriOI GRGCYC 1 cut(s) 930
Fsp4HI GCNGC 3 cut(s) 452, 471, 991
FspBI CTAG 3 cut(s) 8, 1340, 1362
GlaI GCGC 2 cut(s) 945, 1041
GluI GCNGC 3 cut(s) 452, 471, 991
HaeII RGCGCY 1 cut(s) 947
HaeIII GGCC 2 cut(s) 138, 335
HapII CCGG 3 cut(s) 87, 135, 305
HgaI GACGC 1 cut(s) 202
HhaI GCGC 2 cut(s) 946, 1042
Hin1II CATG 4 cut(s) 50, 124, 376, 902
Hin6I GCGC 2 cut(s) 944, 1040
HinP1I GCGC 2 cut(s) 944, 1040
HincII GTYRAC 1 cut(s) 862
HindII GTYRAC 1 cut(s) 862
HinfI GANTC 9 cut(s) 18, 327, 353, 621, 671, 728, 995, 1112, 1193
HpaI GTTAAC 1 cut(s) 862
HpaII CCGG 3 cut(s) 87, 135, 305
HphI GGTGA 5 cut(s) 19, 124, 247, 539, 1345
Hpy166II GTNNAC 2 cut(s) 349, 862
Hpy188I TCNGA 7 cut(s) 69, 287, 629, 715, 768, 925, 1201
Hpy188III TCNNGA 8 cut(s) 8, 22, 227, 243, 305, 364, 1145, 1250
Hpy8I GTNNAC 2 cut(s) 349, 862
Hpy99I CGWCG 1 cut(s) 564
HpyAV CCTTC 8 cut(s) 226, 415, 474, 879, 1076, 1118, 1174, 1252
HpyCH4III ACNGT 3 cut(s) 37, 652, 811
HpyCH4IV ACGT 2 cut(s) 853, 969
HpyCH4V TGCA 4 cut(s) 691, 740, 898, 1091
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 950, 1105, 1313
HpyF3I CTNAG 3 cut(s) 105, 924, 948
HpySE526I ACGT 2 cut(s) 853, 969
Hsp92II CATG 4 cut(s) 50, 124, 376, 902
HspAI GCGC 2 cut(s) 944, 1040
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 1246
KspAI GTTAAC 1 cut(s) 862
Kzo9I GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
LmnI GCTCC 2 cut(s) 599, 933
Lsp1109I GCAGC 3 cut(s) 463, 482, 1002
LweI GCATC 4 cut(s) 213, 559, 1078, 1326
MaeI CTAG 3 cut(s) 8, 1340, 1362
MaeII ACGT 2 cut(s) 853, 969
MaeIII GTNAC 3 cut(s) 823, 847, 886
MalI GATC 9 cut(s) 6, 26, 45, 85, 324, 368, 410, 804, 1248
MboI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
MflI RGATCY 2 cut(s) 4, 83
MhlI GDGCHC 2 cut(s) 930, 1292
MluCI AATT 9 cut(s) 11, 300, 393, 510, 522, 762, 791, 935, 1071
MlyI GAGTC 3 cut(s) 12, 630, 737
MmeI TCCRAC 1 cut(s) 760
Mph1103I ATGCAT 1 cut(s) 900
MroI TCCGGA 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 969
MseI TTAA 4 cut(s) 144, 342, 654, 861
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 3 cut(s) 87, 135, 305
MspR9I CCNGG 1 cut(s) 1331
Mva1269I GAATGC 1 cut(s) 1066
MvaI CCWGG 1 cut(s) 1331
MvnI CGCG 2 cut(s) 399, 1053
MwoI GCNNNNNNNGC 4 cut(s) 460, 950, 1105, 1313
NdeII GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
NheI GCTAGC 1 cut(s) 1339
NlaIII CATG 4 cut(s) 50, 124, 376, 902
NlaIV GGNNCC 1 cut(s) 595
NsiI ATGCAT 1 cut(s) 900
NspI RCATGY 1 cut(s) 124
NspV TTCGAA 3 cut(s) 298, 795, 973
PctI GAATGC 1 cut(s) 1066
PdmI GAANNNNTTC 1 cut(s) 969
PfeI GAWTC 6 cut(s) 327, 353, 671, 995, 1112, 1193
PflMI CCANNNNNTGG 1 cut(s) 937
PkrI GCNGC 3 cut(s) 453, 472, 992
Ple19I CGATCG 1 cut(s) 369
PleI GAGTC 3 cut(s) 12, 629, 736
PmaCI CACGTG 1 cut(s) 854
PmlI CACGTG 1 cut(s) 854
PpsI GAGTC 3 cut(s) 12, 629, 736
Ppu21I YACGTR 1 cut(s) 854
PsiI TTATAA 1 cut(s) 1301
Psp124BI GAGCTC 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 1329
PspCI CACGTG 1 cut(s) 854
PspGI CCWGG 1 cut(s) 1329
PspN4I GGNNCC 1 cut(s) 595
PspPI GGNCC 1 cut(s) 89
PsuI RGATCY 2 cut(s) 4, 83
PvuI CGATCG 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 2 cut(s) 257, 348
RsaNI GTAC 2 cut(s) 256, 347
SacI GAGCTC 1 cut(s) 930
SaqAI TTAA 4 cut(s) 144, 342, 654, 861
SatI GCNGC 3 cut(s) 452, 471, 991
Sau3AI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 802, 1246
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 3 cut(s) 12, 630, 737
ScrFI CCNGG 1 cut(s) 1331
SduI GDGCHC 2 cut(s) 930, 1292
SfaNI GCATC 4 cut(s) 213, 559, 1078, 1326
SfcI CTRYAG 1 cut(s) 810
SfuI TTCGAA 3 cut(s) 298, 795, 973
SinI GGWCC 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 9 cut(s) 11, 300, 393, 510, 522, 762, 791, 935, 1071
SsiI CCGC 5 cut(s) 399, 434, 468, 1064, 1137
SspMI CTAG 3 cut(s) 8, 1340, 1362
SstI GAGCTC 1 cut(s) 930
StyD4I CCNGG 1 cut(s) 1329
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 3 cut(s) 37, 652, 811
TaiI ACGT 2 cut(s) 856, 972
TaqI TCGA 6 cut(s) 298, 325, 411, 562, 795, 973
TaqII GACCGA 1 cut(s) 548
TasI AATT 9 cut(s) 11, 300, 393, 510, 522, 762, 791, 935, 1071
TatI WGTACW 2 cut(s) 255, 346
TfiI GAWTC 6 cut(s) 327, 353, 671, 995, 1112, 1193
Tru1I TTAA 4 cut(s) 144, 342, 654, 861
Tru9I TTAA 4 cut(s) 144, 342, 654, 861
TscAI CASTG 2 cut(s) 214, 390
TseI GCWGC 3 cut(s) 451, 470, 990
TspDTI ATGAA 6 cut(s) 115, 198, 444, 600, 738, 1206
TspRI CASTG 2 cut(s) 214, 390
Van91I CCANNNNNTGG 1 cut(s) 937
VpaK11BI GGWCC 1 cut(s) 89
XagI CCTNNNNNAGG 1 cut(s) 951
XapI RAATTY 4 cut(s) 300, 393, 791, 1071
XbaI TCTAGA 1 cut(s) 7
XceI RCATGY 1 cut(s) 124
XmnI GAANNNNTTC 1 cut(s) 969
XspI CTAG 3 cut(s) 8, 1340, 1362
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.