Rh6DG200400

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0010913
Physical Location & Seq
Forward (+)
35698442 .. 35701271
2830 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG200400.1

Sequence Viewer

Length: 1056 bp
ATGGATCTTGAATTAGGACTCGAGATCACCAAAAATGCAGATGATCATACTTCCTCTTCTGATTTTCGGATTGCGAAGGAGAAATCTGGTCCTGTTTTCATATCCACCGAAACAGAAAGCGCGTACATCCTCACTGCCCATCTTGAAGGTTGTAGAAAAGAGCATGTCAATATTGACATAAATGAAGACGAGACTCAGCTAGGCGTTGCTGTGGAGAAGCCAGTTCAAGAGAAGGTGATGTCAGGATGGTCAATGAACAAGAAAGAAGGGGAGACAAGGGGATTCAGAAAAGTCTTTCGGATCCCAGATGGAGTGGTTTTGAGTCGAATCAAGGCCAAGATTAATGAACAAGACTCGACTCTCAGAATCTTCATGCCAAAAGTGGTGAAAGGATTTGTTGGTGCTGGGGTTGAGGAACTGAAAGAAGAAGAGGTGATCATAGGGCAACCACAGATGACAGAAGCTGCAAGTGATGAGGTTCCTGAAAAAATCAGTGAGAAAATGAAAATGGATAAGAACCCTCAGGCTGAAGGAAAAGAGGTAGATAAAGAAGAGTACACGAAAGAAGTTGAATTAGATGATCAAGATTTGCAGACCAATGATCGAAGAGAAACTAAAGGTGGAGGGATGGAGTCAGAAGAAAGTGAAGAAAGTGAAGAAAGAAAGATTGCAAAGACAAAAAAAGCTGATAGAATTGAGAAACAGAGAGAGGATGCTAAAAAAAGAATCAAGGAAAAAACACATGGAGGAGTCAAGAATGATAAAAGCACTGAAGCAACTCATCAAGTTGCTAAACCATCACAAGAAATTGAAGAAATCAGACCAGAATCGAGACCCCCCAACGCGAACGAAATGCCAAAAGCGACGCCAGAGAAGAAGGAAGTACTTGACAAGGGTGAATCTTCTGAAATGCGGAAAGGAAAAGATGTGTGTGAGAAATCAACTCCAGAGAAGAAGCCTGCTGCGTCAGAAAAGTCCAAGTTGCTGTCCACTCCTTTGATCATTGCAGCATTGACATCCTCCGTGATAGTAAGGGGAATCTTCCTCGCATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

351

Amino Acids

39.28

Weight (kDa)

5.35

Isoelectric Point (pI)

50.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 122, 845
AciI CCGC 1 cut(s) 913
AclWI GGATC 3 cut(s) 12, 295, 308
AcuI CTGAAG 2 cut(s) 549, 792
AcyI GRCGYC 1 cut(s) 866
AfaI GTAC 3 cut(s) 125, 557, 885
AgsI TTSAA 5 cut(s) 11, 146, 227, 572, 812
AluBI AGCT 3 cut(s) 199, 464, 686
AluI AGCT 3 cut(s) 199, 464, 686
Alw26I GTCTC 3 cut(s) 185, 266, 826
AlwI GGATC 3 cut(s) 12, 295, 308
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 3 cut(s) 464, 962, 1007
AseI ATTAAT 1 cut(s) 342
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 19, 247, 397, 445, 908
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 1 cut(s) 522
BamHI GGATCC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 3 cut(s) 451, 949, 1019
BccI CCATC 5 cut(s) 147, 240, 302, 622, 805
BcgI CGANNNNNNTGC 2 cut(s) 835, 869
BclI TGATCA 4 cut(s) 43, 435, 580, 999
BcoDI GTCTC 3 cut(s) 185, 266, 826
BfaI CTAG 1 cut(s) 200
BisI GCNGC 3 cut(s) 465, 963, 1008
BlsI GCNGC 3 cut(s) 466, 964, 1009
BmcAI AGTACT 1 cut(s) 885
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 302, 480
BmsI GCATC 1 cut(s) 703
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 930
BsaHI GRCGYC 1 cut(s) 866
BsaI GGTCTC 1 cut(s) 826
Bse1I ACTGG 1 cut(s) 221
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 1002
BseGI GGATG 5 cut(s) 126, 251, 633, 718, 1016
BseMI GCAATG 1 cut(s) 1002
BseMII CTCAG 3 cut(s) 209, 376, 536
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 762
BseXI GCAGC 3 cut(s) 451, 949, 1019
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 2 cut(s) 122, 845
BshFI GGCC 1 cut(s) 335
BsiHKCI CYCGRG 1 cut(s) 20
BsmAI GTCTC 3 cut(s) 185, 266, 826
BsnI GGCC 1 cut(s) 335
Bso31I GGTCTC 1 cut(s) 826
BsoBI CYCGRG 1 cut(s) 20
Bsp143I GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
BspACI CCGC 1 cut(s) 913
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 3 cut(s) 208, 375, 535
BspFNI CGCG 2 cut(s) 122, 845
BspLI GGNNCC 2 cut(s) 302, 480
BspPI GGATC 3 cut(s) 12, 295, 308
BspTNI GGTCTC 1 cut(s) 826
BsrDI GCAATG 1 cut(s) 1002
BsrI ACTGG 1 cut(s) 221
BssMI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
BssNI GRCGYC 1 cut(s) 866
Bst6I CTCTTC 4 cut(s) 61, 423, 546, 601
BstACI GRCGYC 1 cut(s) 866
BstC8I GCNNGC 1 cut(s) 960
BstDEI CTNAG 3 cut(s) 195, 362, 522
BstF5I GGATG 5 cut(s) 126, 251, 633, 718, 1016
BstFNI CGCG 2 cut(s) 122, 845
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 8 cut(s) 7, 27, 46, 303, 438, 583, 604, 1002
BstMAI GTCTC 3 cut(s) 185, 266, 826
BstMBI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
BstNSI RCATGY 2 cut(s) 167, 1053
BstUI CGCG 2 cut(s) 122, 845
BstV1I GCAGC 3 cut(s) 451, 949, 1019
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 2 cut(s) 4, 300
BstYI RGATCY 2 cut(s) 4, 300
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 335
BtsCI GGATG 5 cut(s) 126, 251, 633, 718, 1016
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 132, 499, 768
Cac8I GCNNGC 1 cut(s) 960
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 2 cut(s) 874, 954
Csp6I GTAC 3 cut(s) 124, 556, 884
CviAII CATG 4 cut(s) 164, 373, 743, 1050
CviJI RGCY 7 cut(s) 199, 220, 335, 464, 527, 686, 958
CviKI_1 RGCY 7 cut(s) 199, 220, 335, 464, 527, 686, 958
CviQI GTAC 3 cut(s) 124, 556, 884
DdeI CTNAG 3 cut(s) 195, 362, 522
DpnI GATC 8 cut(s) 6, 26, 45, 302, 437, 582, 603, 1001
DpnII GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
Eam1104I CTCTTC 4 cut(s) 61, 423, 546, 601
EarI CTCTTC 4 cut(s) 61, 423, 546, 601
Eco31I GGTCTC 1 cut(s) 826
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 549, 792
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 20
FaeI CATG 4 cut(s) 167, 376, 746, 1053
FaiI YATR 8 cut(s) 48, 101, 165, 179, 374, 440, 744, 1051
FatI CATG 4 cut(s) 163, 372, 742, 1049
FbaI TGATCA 4 cut(s) 43, 435, 580, 999
Fnu4HI GCNGC 3 cut(s) 465, 963, 1008
FokI GGATG 5 cut(s) 113, 258, 640, 725, 1003
Fsp4HI GCNGC 3 cut(s) 465, 963, 1008
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 121
GluI GCNGC 3 cut(s) 465, 963, 1008
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 1 cut(s) 335
HgaI GACGC 2 cut(s) 874, 954
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 866
Hin1II CATG 4 cut(s) 167, 376, 746, 1053
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HphI GGTGA 5 cut(s) 19, 247, 397, 445, 908
Hpy166II GTNNAC 2 cut(s) 558, 990
Hpy188I TCNGA 9 cut(s) 61, 69, 287, 300, 365, 637, 821, 907, 970
Hpy8I GTNNAC 2 cut(s) 558, 990
Hpy99I CGWCG 1 cut(s) 868
HpyAV CCTTC 6 cut(s) 70, 140, 226, 260, 524, 871
HpyCH4V TGCA 5 cut(s) 38, 467, 592, 671, 1007
HpyF3I CTNAG 3 cut(s) 195, 362, 522
Hsp92I GRCGYC 1 cut(s) 866
Hsp92II CATG 4 cut(s) 167, 376, 746, 1053
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 4 cut(s) 43, 435, 580, 999
Kzo9I GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
Lsp1109I GCAGC 3 cut(s) 451, 949, 1019
LweI GCATC 1 cut(s) 703
MaeI CTAG 1 cut(s) 200
MalI GATC 8 cut(s) 6, 26, 45, 302, 437, 582, 603, 1001
MboI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
MflI RGATCY 2 cut(s) 4, 300
MluCI AATT 4 cut(s) 11, 572, 693, 807
MlyI GAGTC 7 cut(s) 12, 187, 331, 347, 352, 641, 759
MseI TTAA 1 cut(s) 342
MvnI CGCG 2 cut(s) 122, 845
NdeII GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
NlaIII CATG 4 cut(s) 167, 376, 746, 1053
NlaIV GGNNCC 2 cut(s) 302, 480
NspI RCATGY 2 cut(s) 167, 1053
PaeR7I CTCGAG 1 cut(s) 20
PfeI GAWTC 7 cut(s) 282, 327, 366, 726, 827, 899, 1038
PkrI GCNGC 3 cut(s) 466, 964, 1009
PleI GAGTC 7 cut(s) 12, 187, 330, 347, 352, 640, 758
PpsI GAGTC 7 cut(s) 12, 187, 330, 347, 352, 640, 758
PshBI ATTAAT 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 302, 480
PspPI GGNCC 1 cut(s) 89
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 4, 300
RsaI GTAC 3 cut(s) 125, 557, 885
RsaNI GTAC 3 cut(s) 124, 556, 884
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 3 cut(s) 465, 963, 1008
Sau3AI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 885
SchI GAGTC 7 cut(s) 12, 187, 331, 347, 352, 641, 759
SetI ASST 9 cut(s) 151, 201, 237, 435, 466, 480, 543, 622, 688
SfaNI GCATC 1 cut(s) 703
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 4 cut(s) 11, 572, 693, 807
SsiI CCGC 1 cut(s) 913
SspI AATATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 200
TaqI TCGA 5 cut(s) 21, 325, 356, 604, 830
TasI AATT 4 cut(s) 11, 572, 693, 807
TatI WGTACW 2 cut(s) 555, 883
TfiI GAWTC 7 cut(s) 282, 327, 366, 726, 827, 899, 1038
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 3 cut(s) 139, 499, 775
TseI GCWGC 3 cut(s) 464, 962, 1007
TspDTI ATGAA 6 cut(s) 88, 198, 269, 360, 361, 518
TspGWI ACGGA 1 cut(s) 1012
TspRI CASTG 3 cut(s) 139, 499, 775
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 2 cut(s) 167, 1053
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.