RLG00000013474

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010913
Physical Location & Seq
Reverse (-)
32094162 .. 32095303
1142 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000013474

Sequence Viewer

Length: 1065 bp
ATGGATCTTGAATTAGGACTCAAGATCACCAAAAATGCAGATGATCATACTTCCTCTTCTGATTTTCGGATTGCGAAGGAGAAATCTGGTCCTGTTTTCATATCCACCGAAACAGAAAGCGCGTACATCCTCACTGCCCATCTTAAAGGTTATAGAAAAGAGCATGTTGATATTGACATAAATGAAGACGAGACTCAGCTAGGCATTGCTGTGGAGAAGCCAGTTCAAGAGAAGGTGATGTCAGGATGGTCAATGAACAAGAAAGAAGGGGAGACAAGGGGATTAAGAAAAGTCTTTCGGATCCCAGATGGAGTGGTTTTGAGTCGAATCAAGGCCAAGATTAATGAACAAGACTCGACTCTCAGAATCTTCATGCCAAAAGTGGTGAAAGGATTTGTTGGTGCTGGGGTTGAGGAACTGAAAGAAGAAGAGGTGATCATAGGGAAACCACAGATGACAGAAGCTGCAAGTGATGAGGTTCCTGAAAAAATCAGTGAGAAAACGAAAATGGATAAGAACTCTCAGGCTAAAGGAACAGAGGTAGATAAAGAAGAGTACACGAAAGAAGTTGTATTAGATGATCAAGATTTGCAGACCAATGATCGAAGAGAAACTGAAGGTGGAGGGATGGCGTCAGAAGAAAGTGAAGAACGAAAGATTGCAAAGACTGAAAAAGCTGACAGAATTGAGAAAGAGATAGAGGATGCTAAAAAAAGAATCAAGGAAAAAACACATGGAGGAGTCAAGAATGATAAAAGCACTGAAGCAACTCATCAAGTTGCTAAACCATCACAAGAAATTGAAGAAATCGAACCAGAATTGAGACCCCCCAACGCGAACGAAATGCCAAAAGCAACGCCAGAGAAGGAGGAAGTACTTGACAAGGGTGAATCTTCTGAAATGCGGAAAGGAAACGTTGTGTGTGAGAAATCAACTCCAGAGAAGAAGCCTGCTGCGTCAGAAAAGTCCAAGTTGCTGTCCACTCCTTTGATCATTGCAGGTTCAGCCCTTCTCGTCACTATTGTACTGCTAGCAATTCACAGGATTAGGAAAAGAAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

355

Amino Acids

39.58

Weight (kDa)

5.61

Isoelectric Point (pI)

47.01

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 989
AccII CGCG 2 cut(s) 122, 836
AciI CCGC 1 cut(s) 904
AclI AACGTT 1 cut(s) 915
AclWI GGATC 3 cut(s) 12, 295, 308
AcuI CTGAAG 2 cut(s) 636, 783
AcyI GRCGYC 1 cut(s) 632
AfaI GTAC 4 cut(s) 125, 557, 876, 1026
AgsI TTSAA 3 cut(s) 11, 227, 803
AluBI AGCT 3 cut(s) 199, 464, 677
AluI AGCT 3 cut(s) 199, 464, 677
Alw26I GTCTC 3 cut(s) 185, 266, 817
AlwI GGATC 3 cut(s) 12, 295, 308
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 464, 953
AseI ATTAAT 1 cut(s) 342
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 19, 247, 397, 445, 899
AsuNHI GCTAGC 1 cut(s) 1030
AvaII GGWCC 1 cut(s) 89
BamHI GGATCC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 451, 940
BccI CCATC 5 cut(s) 147, 240, 302, 622, 796
BcgI CGANNNNNNTGC 2 cut(s) 826, 860
BclI TGATCA 4 cut(s) 43, 435, 580, 990
BcoDI GTCTC 3 cut(s) 185, 266, 817
BfaI CTAG 2 cut(s) 200, 1031
BfuAI ACCTGC 1 cut(s) 989
BisI GCNGC 2 cut(s) 465, 954
BlsI GCNGC 2 cut(s) 466, 955
BmcAI AGTACT 1 cut(s) 876
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 302, 480
BmsI GCATC 1 cut(s) 694
BmtI GCTAGC 1 cut(s) 1034
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 921
BpuEI CTTGAG 1 cut(s) 5
BsaHI GRCGYC 1 cut(s) 632
BsaI GGTCTC 1 cut(s) 817
Bse1I ACTGG 1 cut(s) 221
Bse3DI GCAATG 2 cut(s) 204, 993
BseGI GGATG 4 cut(s) 126, 251, 633, 709
BseMI GCAATG 2 cut(s) 204, 993
BseMII CTCAG 3 cut(s) 209, 376, 536
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 753
BseXI GCAGC 2 cut(s) 451, 940
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 2 cut(s) 122, 836
BshFI GGCC 1 cut(s) 335
BsmAI GTCTC 3 cut(s) 185, 266, 817
BsnI GGCC 1 cut(s) 335
Bso31I GGTCTC 1 cut(s) 817
Bsp143I GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
BspACI CCGC 1 cut(s) 904
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 3 cut(s) 208, 375, 535
BspFNI CGCG 2 cut(s) 122, 836
BspLI GGNNCC 2 cut(s) 302, 480
BspMI ACCTGC 1 cut(s) 989
BspOI GCTAGC 1 cut(s) 1034
BspPI GGATC 3 cut(s) 12, 295, 308
BspTNI GGTCTC 1 cut(s) 817
BsrDI GCAATG 2 cut(s) 204, 993
BsrI ACTGG 1 cut(s) 221
BssMI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
BssNI GRCGYC 1 cut(s) 632
Bst6I CTCTTC 4 cut(s) 61, 423, 546, 601
BstACI GRCGYC 1 cut(s) 632
BstC8I GCNNGC 2 cut(s) 951, 1032
BstDEI CTNAG 3 cut(s) 195, 362, 522
BstF5I GGATG 4 cut(s) 126, 251, 633, 709
BstFNI CGCG 2 cut(s) 122, 836
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 8 cut(s) 7, 27, 46, 303, 438, 583, 604, 993
BstMAI GTCTC 3 cut(s) 185, 266, 817
BstMBI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
BstMWI GCNNNNNNNGC 1 cut(s) 1004
BstNSI RCATGY 1 cut(s) 167
BstUI CGCG 2 cut(s) 122, 836
BstV1I GCAGC 2 cut(s) 451, 940
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 2 cut(s) 4, 300
BstYI RGATCY 2 cut(s) 4, 300
BsuRI GGCC 1 cut(s) 335
BtsCI GGATG 4 cut(s) 126, 251, 633, 709
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 132, 499, 759
BveI ACCTGC 1 cut(s) 989
Cac8I GCNNGC 2 cut(s) 951, 1032
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 2 cut(s) 621, 945
Csp6I GTAC 4 cut(s) 124, 556, 875, 1025
CviAII CATG 3 cut(s) 164, 373, 734
CviJI RGCY 8 cut(s) 199, 220, 335, 464, 527, 677, 949, 1007
CviKI_1 RGCY 8 cut(s) 199, 220, 335, 464, 527, 677, 949, 1007
CviQI GTAC 4 cut(s) 124, 556, 875, 1025
DdeI CTNAG 3 cut(s) 195, 362, 522
DpnI GATC 8 cut(s) 6, 26, 45, 302, 437, 582, 603, 992
DpnII GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
Eam1104I CTCTTC 4 cut(s) 61, 423, 546, 601
EarI CTCTTC 4 cut(s) 61, 423, 546, 601
Eco31I GGTCTC 1 cut(s) 817
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 636, 783
FaeI CATG 3 cut(s) 167, 376, 737
FaiI YATR 8 cut(s) 48, 101, 153, 165, 179, 374, 440, 735
FatI CATG 3 cut(s) 163, 372, 733
FbaI TGATCA 4 cut(s) 43, 435, 580, 990
Fnu4HI GCNGC 2 cut(s) 465, 954
FokI GGATG 4 cut(s) 113, 258, 640, 716
Fsp4HI GCNGC 2 cut(s) 465, 954
FspBI CTAG 2 cut(s) 200, 1031
GlaI GCGC 1 cut(s) 121
GluI GCNGC 2 cut(s) 465, 954
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 921
HaeIII GGCC 1 cut(s) 335
HgaI GACGC 2 cut(s) 621, 945
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 632
Hin1II CATG 3 cut(s) 167, 376, 737
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HphI GGTGA 5 cut(s) 19, 247, 397, 445, 899
Hpy166II GTNNAC 2 cut(s) 558, 981
Hpy188I TCNGA 7 cut(s) 61, 69, 300, 365, 637, 898, 961
Hpy188III TCNNGA 8 cut(s) 8, 22, 227, 243, 482, 584, 745, 938
Hpy8I GTNNAC 2 cut(s) 558, 981
HpyAV CCTTC 6 cut(s) 70, 226, 260, 611, 859, 1019
HpyCH4IV ACGT 1 cut(s) 915
HpyCH4V TGCA 5 cut(s) 38, 467, 592, 662, 998
HpyF10VI GCNNNNNNNGC 1 cut(s) 1004
HpyF3I CTNAG 3 cut(s) 195, 362, 522
HpySE526I ACGT 1 cut(s) 915
Hsp92I GRCGYC 1 cut(s) 632
Hsp92II CATG 3 cut(s) 167, 376, 737
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 4 cut(s) 43, 435, 580, 990
Kzo9I GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
Lsp1109I GCAGC 2 cut(s) 451, 940
LweI GCATC 1 cut(s) 694
MaeI CTAG 2 cut(s) 200, 1031
MaeII ACGT 1 cut(s) 915
MaeIII GTNAC 1 cut(s) 1015
MalI GATC 8 cut(s) 6, 26, 45, 302, 437, 582, 603, 992
MboI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
MflI RGATCY 2 cut(s) 4, 300
MluCI AATT 5 cut(s) 11, 684, 798, 818, 1035
MlyI GAGTC 6 cut(s) 12, 187, 331, 347, 352, 750
MseI TTAA 3 cut(s) 144, 284, 342
MslI CAYNNNNRTG 1 cut(s) 209
MvnI CGCG 2 cut(s) 122, 836
MwoI GCNNNNNNNGC 1 cut(s) 1004
NdeII GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
NheI GCTAGC 1 cut(s) 1030
NlaIII CATG 3 cut(s) 167, 376, 737
NlaIV GGNNCC 2 cut(s) 302, 480
NmuCI GTSAC 1 cut(s) 1015
NspI RCATGY 1 cut(s) 167
PfeI GAWTC 4 cut(s) 327, 366, 717, 890
PkrI GCNGC 2 cut(s) 466, 955
PleI GAGTC 6 cut(s) 12, 187, 330, 347, 352, 749
PpsI GAGTC 6 cut(s) 12, 187, 330, 347, 352, 749
PshBI ATTAAT 1 cut(s) 342
Psp1406I AACGTT 1 cut(s) 915
PspFI CCCAGC 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 302, 480
PspPI GGNCC 1 cut(s) 89
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 4, 300
RsaI GTAC 4 cut(s) 125, 557, 876, 1026
RsaNI GTAC 4 cut(s) 124, 556, 875, 1025
RseI CAYNNNNRTG 1 cut(s) 209
SaqAI TTAA 3 cut(s) 144, 284, 342
SatI GCNGC 2 cut(s) 465, 954
Sau3AI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 990
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 876
SchI GAGTC 6 cut(s) 12, 187, 331, 347, 352, 750
SfaNI GCATC 1 cut(s) 694
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 209
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 5 cut(s) 11, 684, 798, 818, 1035
SsiI CCGC 1 cut(s) 904
SspMI CTAG 2 cut(s) 200, 1031
TaiI ACGT 1 cut(s) 918
TaqI TCGA 4 cut(s) 325, 356, 604, 810
TasI AATT 5 cut(s) 11, 684, 798, 818, 1035
TatI WGTACW 3 cut(s) 555, 874, 1024
TfiI GAWTC 4 cut(s) 327, 366, 717, 890
Tru1I TTAA 3 cut(s) 144, 284, 342
Tru9I TTAA 3 cut(s) 144, 284, 342
TscAI CASTG 3 cut(s) 139, 499, 766
TseFI GTSAC 1 cut(s) 1015
TseI GCWGC 2 cut(s) 464, 953
Tsp45I GTSAC 1 cut(s) 1015
TspDTI ATGAA 5 cut(s) 88, 198, 269, 360, 361
TspRI CASTG 3 cut(s) 139, 499, 766
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 1 cut(s) 167
XspI CTAG 2 cut(s) 200, 1031
ZrmI AGTACT 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.