MD10G1026100.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010913
Physical Location & Seq
Reverse (-)
3233467 .. 3234967
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1026100.v1.1.491

Sequence Viewer

Length: 1410 bp
ATGGATCTAGAATTAGGACTCAAGATCACCCACAGCGTAGACGATCATGTTTCGTCTGCCAACCTTCGGATTGCCAAGGACAAATCCGGTCCTATTTTCGTGTCTAAGGAAACCGAAACCATGTTCATCCTCACTGCCCATCTCAAAGGTTATAGAAGAGAGCGTGTACATATCGACATAAACGAAGATGGGACTCAGATTGGAATCAGTGGGGAGAAGCCAGTTCAAGAGAAGGTTATGTCAGGATGGATAATGTACAAGAAAGAGGTGGAGATGAGGGGTTTCCGAAAAGTCTTTCGAATTCCGGATAGAGTGGTTTTGGATCGAATCAAGGCCAAGTTTAATGCACACGAATCAACTCTCACGATCGTCATGCCAAAATCGGTGAAAGGAATTCGCGGGGTTGAGATCGAGGAAGTGAAAGAAGATGAAGTGGATATAGGGAGACAGCAGCTCACAAAACCTCCTGCGGCTGGAGAAGTCTCTGAAAAATATGGTTTTAGGGGGAAAATTGAGGACCAATTTGGTGAGCTTGAGAAAAAGAAAGTAGAAGAAAATCATCGAACAGATGCGAAAGGGGTTGATGAAGAGGGCTCCAAGAAAGAAATTGTAGCTGATGGAGCTATCGAAAAGGATGCGAGCAGAGGCGTAGTGCAGGAAGAAGCTAAAGATTCAAAGACTCAAAGTATGCAACACCAAACAAAATCTGTTTCGGAACACAGTGAAGAGCTAAAAGTTGCAAAGGTTAAAGAAGCTGATGGAGTTGAGAAAGAGAAAGTTGAAAGAAAGGAACTCGGAAGAGACCAAATTGTAGCGGACAAGGCTCCAAGGATCAGCAAGGATGCTGAAGAGCCAGTGAATGTGGATAAAACCAGTGAAGTAACTCATGAAGTTACAAGGTCATTACAAGAAGCTCAGGCAATTGCTAAGCATGAAACAGCTGAAGAATACTCTACTGCAGAGCCCGAAATAGAAACACCAAAGGCGGGGATTGAGGAACACGTTAGAAGTAGACCAGAGAGCGGCAAATCATCAAAACTTGCACAAGACAGAAGAGCAACACAAGAAGAAGCACAAGTCGGACACGAAAAGAATACGGACGGAGTTCGTAAAGAAGGTGATATTGTTCAGCAGCTACCAAAGCAAGAACCCATCGAAAAAGGTCACGAAAACAAAATCGAAGAAGTATCAAAGAATGAAGAACAAAATGAAGAGAAGGAAGAGGATGAATCTTCCGAAACGGAGGAACAAGGTGATGTGGCTGATGAGGAATCAAGTGAGGATATGGGGAAGAAGGATGGTTTGGAAAAGTCTAATCTGCTGTGCTCTCCTTTTATCATTGCAGGTTCAGCCCTTATTGTGTCCCTGCTGGTGCTAGCAATCAGCAGGATTAGAACCAGAAAAATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

470

Amino Acids

52.57

Weight (kDa)

5.2

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1334
AccBSI CCGCTC 1 cut(s) 1023
AccI GTMKAC 2 cut(s) 39, 1012
AccII CGCG 1 cut(s) 399
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 5 cut(s) 399, 470, 815, 986, 1023
AclWI GGATC 3 cut(s) 12, 330, 839
AcsI RAATTY 2 cut(s) 300, 393
AcuI CTGAAG 2 cut(s) 867, 963
AfaI GTAC 2 cut(s) 168, 257
AfiI CCNNNNNNNGG 4 cut(s) 66, 473, 986, 1022
AflIII ACRYGT 1 cut(s) 1000
AgsI TTSAA 3 cut(s) 227, 675, 782
AjuI GAANNNNNNNTTGG 2 cut(s) 1286, 1318
Alw21I GWGCWC 1 cut(s) 1328
Alw26I GTCTC 3 cut(s) 439, 487, 795
AlwI GGATC 3 cut(s) 12, 330, 839
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 451, 1132
ApoI RAATTY 2 cut(s) 300, 393
AspS9I GGNCC 2 cut(s) 89, 517
AsuHPI GGTGA 5 cut(s) 19, 397, 539, 1130, 1265
AsuII TTCGAA 1 cut(s) 298
AsuNHI GCTAGC 1 cut(s) 1375
AvaII GGWCC 2 cut(s) 89, 517
BanII GRGCYC 2 cut(s) 596, 966
Bbv12I GWGCWC 1 cut(s) 1328
BbvI GCAGC 2 cut(s) 463, 1144
BccI CCATC 7 cut(s) 147, 182, 240, 611, 752, 1160, 1292
BcgI CGANNNNNNTGC 4 cut(s) 355, 389, 617, 651
BcoDI GTCTC 3 cut(s) 439, 487, 795
BfaI CTAG 2 cut(s) 8, 1376
BfmI CTRYAG 1 cut(s) 957
BfuAI ACCTGC 1 cut(s) 1334
BisI GCNGC 4 cut(s) 452, 471, 1024, 1133
BlpI GCTNAGC 1 cut(s) 927
BlsI GCNGC 4 cut(s) 453, 472, 1025, 1134
Bme18I GGWCC 2 cut(s) 89, 517
BmgT120I GGNCC 2 cut(s) 89, 517
BmiI GGNNCC 2 cut(s) 595, 825
BmsI GCATC 3 cut(s) 559, 625, 832
BmtI GCTAGC 1 cut(s) 1379
BpmI CTGGAG 1 cut(s) 495
Bpu10I CCTNAGC 1 cut(s) 915
Bpu1102I GCTNAGC 1 cut(s) 927
Bpu14I TTCGAA 1 cut(s) 298
BpuEI CTTGAG 2 cut(s) 5, 554
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 795
BsaJI CCNNGG 2 cut(s) 75, 827
BsaWI WCCGGW 2 cut(s) 86, 304
BsaXI ACNNNNNCTCC 2 cut(s) 448, 478
Bsc4I CCNNNNNNNGG 4 cut(s) 66, 473, 986, 1022
Bse1I ACTGG 3 cut(s) 221, 854, 873
Bse3DI GCAATG 1 cut(s) 1338
Bse8I GATNNNNATC 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 75, 827
BseGI GGATG 6 cut(s) 126, 251, 640, 847, 1231, 1303
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 4 cut(s) 66, 473, 986, 1022
BseMI GCAATG 1 cut(s) 1338
BseMII CTCAG 2 cut(s) 209, 929
BseNI ACTGG 3 cut(s) 221, 854, 873
BseXI GCAGC 2 cut(s) 463, 1144
BsgI GTGCAG 1 cut(s) 674
Bsh1236I CGCG 1 cut(s) 399
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 1 cut(s) 335
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 1 cut(s) 1328
BsiSI CCGG 2 cut(s) 87, 305
BslFI GGGAC 2 cut(s) 205, 1348
BslI CCNNNNNNNGG 4 cut(s) 66, 473, 986, 1022
BsmAI GTCTC 3 cut(s) 439, 487, 795
BsmFI GGGAC 2 cut(s) 205, 1348
BsnI GGCC 1 cut(s) 335
Bso31I GGTCTC 1 cut(s) 795
Bsp119I TTCGAA 1 cut(s) 298
Bsp1286I GDGCHC 3 cut(s) 596, 966, 1328
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 2 cut(s) 166, 255
Bsp143I GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
Bsp1720I GCTNAGC 1 cut(s) 927
BspACI CCGC 5 cut(s) 399, 470, 815, 986, 1023
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 2 cut(s) 208, 928
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 1 cut(s) 399
BspHI TCATGA 1 cut(s) 886
BspLI GGNNCC 2 cut(s) 595, 825
BspMAI CTGCAG 1 cut(s) 961
BspMI ACCTGC 1 cut(s) 1334
BspOI GCTAGC 1 cut(s) 1379
BspPI GGATC 3 cut(s) 12, 330, 839
BspQI GCTCTTC 3 cut(s) 720, 843, 1048
BspT104I TTCGAA 1 cut(s) 298
BspTNI GGTCTC 1 cut(s) 795
BsrBI CCGCTC 1 cut(s) 1023
BsrDI GCAATG 1 cut(s) 1338
BsrGI TGTACA 2 cut(s) 166, 255
BsrI ACTGG 3 cut(s) 221, 854, 873
BssECI CCNNGG 2 cut(s) 75, 827
BssMI GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
BssT1I CCWWGG 2 cut(s) 75, 827
Bst4CI ACNGT 1 cut(s) 722
Bst6I CTCTTC 8 cut(s) 151, 582, 720, 793, 843, 1048, 1206, 1215
BstAUI TGTACA 2 cut(s) 166, 255
BstBI TTCGAA 1 cut(s) 298
BstC8I GCNNGC 2 cut(s) 640, 1377
BstDEI CTNAG 4 cut(s) 105, 195, 915, 927
BstF5I GGATG 6 cut(s) 126, 251, 640, 847, 1231, 1303
BstFNI CGCG 1 cut(s) 399
BstKTI GATC 7 cut(s) 7, 27, 46, 325, 369, 411, 834
BstMAI GTCTC 3 cut(s) 439, 487, 795
BstMBI GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 620, 821, 1141, 1349
BstSFI CTRYAG 1 cut(s) 957
BstUI CGCG 1 cut(s) 399
BstV1I GCAGC 2 cut(s) 463, 1144
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 335
BtsCI GGATG 6 cut(s) 126, 251, 640, 847, 1231, 1303
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 5 cut(s) 132, 214, 727, 861, 880
BveI ACCTGC 1 cut(s) 1334
Cac8I GCNNGC 2 cut(s) 640, 1377
CciI TCATGA 1 cut(s) 886
Cfr13I GGNCC 2 cut(s) 89, 517
Csp6I GTAC 2 cut(s) 167, 256
CviAII CATG 5 cut(s) 47, 121, 373, 887, 932
CviQI GTAC 2 cut(s) 167, 256
DdeI CTNAG 4 cut(s) 105, 195, 915, 927
DpnI GATC 7 cut(s) 6, 26, 45, 324, 368, 410, 833
DpnII GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
Eam1104I CTCTTC 8 cut(s) 151, 582, 720, 793, 843, 1048, 1206, 1215
EarI CTCTTC 8 cut(s) 151, 582, 720, 793, 843, 1048, 1206, 1215
Eco130I CCWWGG 2 cut(s) 75, 827
Eco24I GRGCYC 2 cut(s) 596, 966
Eco31I GGTCTC 1 cut(s) 795
Eco47I GGWCC 2 cut(s) 89, 517
Eco57I CTGAAG 2 cut(s) 867, 963
EcoRI GAATTC 2 cut(s) 300, 393
EcoT14I CCWWGG 2 cut(s) 75, 827
EcoT38I GRGCYC 2 cut(s) 596, 966
ErhI CCWWGG 2 cut(s) 75, 827
FaeI CATG 5 cut(s) 50, 124, 376, 890, 935
FaqI GGGAC 2 cut(s) 205, 1348
FatI CATG 5 cut(s) 46, 120, 372, 886, 931
FauI CCCGC 2 cut(s) 392, 979
FblI GTMKAC 2 cut(s) 39, 1012
Fnu4HI GCNGC 4 cut(s) 452, 471, 1024, 1133
FokI GGATG 6 cut(s) 113, 258, 647, 854, 1238, 1310
FriOI GRGCYC 2 cut(s) 596, 966
Fsp4HI GCNGC 4 cut(s) 452, 471, 1024, 1133
FspBI CTAG 2 cut(s) 8, 1376
GluI GCNGC 4 cut(s) 452, 471, 1024, 1133
GsuI CTGGAG 1 cut(s) 495
HaeIII GGCC 1 cut(s) 335
HapII CCGG 2 cut(s) 87, 305
Hin1II CATG 5 cut(s) 50, 124, 376, 890, 935
HinfI GANTC 9 cut(s) 18, 193, 204, 327, 353, 671, 679, 1229, 1271
HpaII CCGG 2 cut(s) 87, 305
HphI GGTGA 5 cut(s) 19, 397, 539, 1130, 1265
Hpy166II GTNNAC 3 cut(s) 40, 167, 1013
Hpy188I TCNGA 8 cut(s) 69, 198, 287, 487, 715, 797, 1082, 1237
Hpy188III TCNNGA 8 cut(s) 8, 22, 227, 243, 305, 364, 887, 1166
Hpy8I GTNNAC 3 cut(s) 40, 167, 1013
HpyAV CCTTC 5 cut(s) 74, 226, 1109, 1210, 1288
HpyCH4III ACNGT 1 cut(s) 722
HpyCH4IV ACGT 1 cut(s) 1002
HpyCH4V TGCA 7 cut(s) 347, 655, 691, 740, 959, 1043, 1343
HpyF10VI GCNNNNNNNGC 4 cut(s) 620, 821, 1141, 1349
HpyF3I CTNAG 4 cut(s) 105, 195, 915, 927
HpySE526I ACGT 1 cut(s) 1002
Hsp92II CATG 5 cut(s) 50, 124, 376, 890, 935
Kpn2I TCCGGA 1 cut(s) 304
Kzo9I GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
LguI GCTCTTC 3 cut(s) 720, 843, 1048
LmnI GCTCC 3 cut(s) 599, 620, 829
Lsp1109I GCAGC 2 cut(s) 463, 1144
LweI GCATC 3 cut(s) 559, 625, 832
MaeI CTAG 2 cut(s) 8, 1376
MaeII ACGT 1 cut(s) 1002
MaeIII GTNAC 3 cut(s) 880, 892, 1163
MalI GATC 7 cut(s) 6, 26, 45, 324, 368, 410, 833
MbiI CCGCTC 1 cut(s) 1023
MboI GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
MfeI CAATTG 1 cut(s) 921
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 596, 966, 1328
MluCI AATT 8 cut(s) 11, 300, 393, 510, 521, 606, 807, 921
MlyI GAGTC 3 cut(s) 12, 187, 673
MmeI TCCRAC 1 cut(s) 1060
MroI TCCGGA 1 cut(s) 304
MseI TTAA 2 cut(s) 342, 747
MspA1I CMGCKG 1 cut(s) 941
MspI CCGG 2 cut(s) 87, 305
MunI CAATTG 1 cut(s) 921
MvnI CGCG 1 cut(s) 399
MwoI GCNNNNNNNGC 4 cut(s) 620, 821, 1141, 1349
NdeII GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
NheI GCTAGC 1 cut(s) 1375
NlaIII CATG 5 cut(s) 50, 124, 376, 890, 935
NlaIV GGNNCC 2 cut(s) 595, 825
NmuCI GTSAC 1 cut(s) 1163
NspV TTCGAA 1 cut(s) 298
PagI TCATGA 1 cut(s) 886
PciSI GCTCTTC 3 cut(s) 720, 843, 1048
PcsI WCGNNNNNNNCGW 1 cut(s) 180
PfeI GAWTC 6 cut(s) 204, 327, 353, 671, 1229, 1271
PkrI GCNGC 4 cut(s) 453, 472, 1025, 1134
Ple19I CGATCG 1 cut(s) 369
PleI GAGTC 3 cut(s) 12, 187, 673
PpsI GAGTC 3 cut(s) 12, 187, 673
PspN4I GGNNCC 2 cut(s) 595, 825
PspPI GGNCC 2 cut(s) 89, 517
PstI CTGCAG 1 cut(s) 961
PsuI RGATCY 1 cut(s) 4
PvuI CGATCG 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 941
RsaI GTAC 2 cut(s) 168, 257
RsaNI GTAC 2 cut(s) 167, 256
SapI GCTCTTC 3 cut(s) 720, 843, 1048
SaqAI TTAA 2 cut(s) 342, 747
SatI GCNGC 4 cut(s) 452, 471, 1024, 1133
Sau3AI GATC 7 cut(s) 4, 24, 43, 322, 366, 408, 831
Sau96I GGNCC 2 cut(s) 89, 517
SchI GAGTC 3 cut(s) 12, 187, 673
SduI GDGCHC 3 cut(s) 596, 966, 1328
SfaNI GCATC 3 cut(s) 559, 625, 832
SfcI CTRYAG 1 cut(s) 957
SfuI TTCGAA 1 cut(s) 298
SinI GGWCC 2 cut(s) 89, 517
SmlI CTYRAG 2 cut(s) 20, 533
SmoI CTYRAG 2 cut(s) 20, 533
Sse9I AATT 8 cut(s) 11, 300, 393, 510, 521, 606, 807, 921
SsiI CCGC 5 cut(s) 399, 470, 815, 986, 1023
SspMI CTAG 2 cut(s) 8, 1376
StyI CCWWGG 2 cut(s) 75, 827
TaaI ACNGT 1 cut(s) 722
TaiI ACGT 1 cut(s) 1005
TaqI TCGA 8 cut(s) 174, 298, 325, 411, 562, 627, 1155, 1179
TasI AATT 8 cut(s) 11, 300, 393, 510, 521, 606, 807, 921
TatI WGTACW 2 cut(s) 166, 255
TauI GCSGC 2 cut(s) 473, 1026
TfiI GAWTC 6 cut(s) 204, 327, 353, 671, 1229, 1271
Tru1I TTAA 2 cut(s) 342, 747
Tru9I TTAA 2 cut(s) 342, 747
TscAI CASTG 5 cut(s) 139, 214, 727, 861, 880
TseFI GTSAC 1 cut(s) 1163
TseI GCWGC 2 cut(s) 451, 1132
Tsp45I GTSAC 1 cut(s) 1163
TspDTI ATGAA 8 cut(s) 115, 444, 600, 903, 948, 1212, 1224, 1242
TspGWI ACGGA 3 cut(s) 1112, 1116, 1256
TspRI CASTG 5 cut(s) 139, 214, 727, 861, 880
VpaK11BI GGWCC 2 cut(s) 89, 517
XapI RAATTY 2 cut(s) 300, 393
XbaI TCTAGA 1 cut(s) 7
XmiI GTMKAC 2 cut(s) 39, 1012
XspI CTAG 2 cut(s) 8, 1376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.