Prupe.8G031300_v2.0.a1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0010913
Physical Location & Seq
Reverse (-)
2963581 .. 2966545
2965 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G031300.1

Sequence Viewer

Length: 1458 bp
ATGGATCTAGAATTGAGACTCAAGATCACCCATAACATAGATGATCATGTTTCTTCTGCCAACTTTCAGATTGCCAAAGACAATTCCGATCCTCTTTTTGTATCGAAAGAAACCGAAACTACCTTCGTCCTCACTGGTCATCTCAAAGGTTATAGAAGACAGCATATTGAAATTGACATAAATGAAGATGGGACTCAGATTGCCATTAGTGGGAAAAAGCCAGTTCAAGAGAAGGTGATGATAAGATGGATAATGTACAAGAAAGAGGTGGAGATAAGGAGTTTCAGAAAAGTCTTTCGGATTCCGGATGGAGTGGTTTTGGATCGAATCAAGGCCAAGTTTAAGGAACACGAATCAACTCTCGCCATCGTCATGCCAAAATCAGAGAAAGGTATTCGAGGGGTTGGGATTGAGGAAGTGAAAGATGATGGGGTGGATAAAGGACTAGAAACACAGCAGATGGCACAACCTGCAGCTGAAGAGGTTCCTGAAAAAAATGGTTCTAGGGGGAAACTTGAGGTAGAATTTGTTGAGGCTGAGGAAAAGAAAATGGAAGAAAATCGTCAGATCAAGGGAAAAGAAGTTGATGAAGAGGTGTCCAAGAAAGAAACTGTAGCTGATGGAGTTCTTGGGAAGGACATGAGTGGGGGAAAAAATCAGGAAGAAGCTAGAGCTTCGAAGATACAGAGTATGCAAGAGACAGAAAAGTCTGTATCGAGAAATAGAGAAGAGCCAAAAATGGCAAGGATTGCGAAGATTGAAGAAGCTGATGGTGTTGAGAAAGAGACGGTTGCTAGAAAGGAATTAGGCACAGAACAAATTGTAACTGATTATAAGGTGCCAAAGACTGAGGATGCTAAAGAGACAATGCAGGAAGATACAGGCACTCGAGTAAGTCAAGAAGTTGCTAAGCCAACGCAAGAAACTCAGGCAACCATACATCACACTGAACAGAAGGGTTCTGAGCTCCCCAAATTGGAAGAGCAGGTGCAGAAGCAGAGAAGTCCAGAAGCTGATCTAAGCAAAAAGCGAAATGCGGGGCTGGAGGATGACATTCGTAGTAGACGAGAGAGCAGTGACTCATCAAAACCTGCAGGAGATACATTAACCACACAACAAGAAGCTCAAGCTCAACAGATAGAGACTACAGATGGAATTAGTACAGAAGCAGATGTTGCTCAGCTGCAAAAGCAAGAACCCATCAAAGAATGTCCTGCTAGCAATCAGTTACCTGTCAAAGCAGAAGGAAGCCATGGAAATGAAATTCAAGAAGCAGGAACCAATGAAGAACACATGAAAGAGAAGGAAGTTGAGAAATCAGGTGATGATCTAGAGAAGACGCCTCCTCGTTTGGAAAAATCTAATCTGCTGTGCTCTCCATTCATCATTGCAGGTTCAGCACTAATTGTTTCCCTAGTCATGGTAGCAATTAACAGGATTAGAACTAGAAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

486

Amino Acids

54.4

Weight (kDa)

5.36

Isoelectric Point (pI)

50.45

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 834
AarI CACCTGC 1 cut(s) 976
AasI GACNNNNNNGTC 1 cut(s) 706
Acc36I ACCTGC 4 cut(s) 478, 976, 1099, 1382
AccB1I GGYRCC 1 cut(s) 838
AccI GTMKAC 1 cut(s) 1063
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 1 cut(s) 1037
AclWI GGATC 3 cut(s) 12, 83, 330
AcsI RAATTY 2 cut(s) 524, 1263
AcuI CTGAAG 1 cut(s) 498
AcyI GRCGYC 1 cut(s) 1340
AfaI GTAC 2 cut(s) 257, 1162
AfiI CCNNNNNNNGG 3 cut(s) 210, 976, 1420
AgsI TTSAA 4 cut(s) 170, 227, 761, 1268
Alw21I GWGCWC 2 cut(s) 969, 1376
Alw26I GTCTC 5 cut(s) 10, 692, 779, 857, 1136
AlwI GGATC 3 cut(s) 12, 83, 330
AlwNI CAGNNNCTG 1 cut(s) 1013
Ama87I CYCGRG 1 cut(s) 888
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 473, 1183
ApoI RAATTY 2 cut(s) 524, 1263
Asp700I GAANNNNTTC 1 cut(s) 483
AsuHPI GGTGA 3 cut(s) 19, 247, 1334
AsuII TTCGAA 1 cut(s) 677
AsuNHI GCTAGC 1 cut(s) 1217
AvaI CYCGRG 1 cut(s) 888
BanI GGYRCC 1 cut(s) 838
BanII GRGCYC 1 cut(s) 969
BbsI GAAGAC 2 cut(s) 163, 1343
Bbv12I GWGCWC 2 cut(s) 969, 1376
BbvCI CCTCAGC 1 cut(s) 537
BbvI GCAGC 2 cut(s) 485, 1170
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 5 cut(s) 10, 692, 779, 857, 1136
BfaI CTAG 9 cut(s) 8, 446, 504, 669, 795, 1218, 1331, 1415, 1446
BfmI CTRYAG 4 cut(s) 471, 612, 1092, 1146
BfuAI ACCTGC 4 cut(s) 478, 976, 1099, 1382
BisI GCNGC 2 cut(s) 474, 1184
BlpI GCTNAGC 2 cut(s) 909, 1179
BlsI GCNGC 2 cut(s) 475, 1185
BmeT110I CYCGRG 1 cut(s) 888
BmiI GGNNCC 3 cut(s) 486, 840, 1279
BmsI GCATC 1 cut(s) 844
BmtI GCTAGC 1 cut(s) 1221
BpiI GAAGAC 2 cut(s) 163, 1343
BpmI CTGGAG 1 cut(s) 1064
Bpu10I CCTNAGC 1 cut(s) 537
Bpu1102I GCTNAGC 2 cut(s) 909, 1179
Bpu14I TTCGAA 1 cut(s) 677
BpuEI CTTGAG 3 cut(s) 5, 536, 1110
BsaHI GRCGYC 1 cut(s) 1340
BsaJI CCNNGG 1 cut(s) 1252
BsaWI WCCGGW 1 cut(s) 304
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 976, 1420
Bse1I ACTGG 2 cut(s) 139, 221
Bse3DI GCAATG 1 cut(s) 1386
BseAI TCCGGA 1 cut(s) 304
BseDI CCNNGG 1 cut(s) 1252
BseGI GGATG 3 cut(s) 313, 859, 1054
BseLI CCNNNNNNNGG 3 cut(s) 210, 976, 1420
BseMI GCAATG 1 cut(s) 1386
BseMII CTCAG 6 cut(s) 209, 528, 840, 941, 954, 1193
BseNI ACTGG 2 cut(s) 139, 221
BseRI GAGGAG 1 cut(s) 1335
BseXI GCAGC 2 cut(s) 485, 1170
BsgI GTGCAG 1 cut(s) 1010
BshFI GGCC 1 cut(s) 335
BshNI GGYRCC 1 cut(s) 838
BsiHKAI GWGCWC 2 cut(s) 969, 1376
BsiHKCI CYCGRG 1 cut(s) 888
BsiSI CCGG 1 cut(s) 305
BslFI GGGAC 1 cut(s) 205
BslI CCNNNNNNNGG 3 cut(s) 210, 976, 1420
BsmAI GTCTC 5 cut(s) 10, 692, 779, 857, 1136
BsmBI CGTCTC 1 cut(s) 779
BsmFI GGGAC 1 cut(s) 205
BsnI GGCC 1 cut(s) 335
BsoBI CYCGRG 1 cut(s) 888
Bsp119I TTCGAA 1 cut(s) 677
Bsp1286I GDGCHC 2 cut(s) 969, 1376
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
Bsp1720I GCTNAGC 2 cut(s) 909, 1179
Bsp19I CCATGG 1 cut(s) 1252
BspACI CCGC 1 cut(s) 1037
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 6 cut(s) 208, 529, 841, 940, 955, 1192
BspEI TCCGGA 1 cut(s) 304
BspLI GGNNCC 3 cut(s) 486, 840, 1279
BspMAI CTGCAG 2 cut(s) 475, 1096
BspMI ACCTGC 4 cut(s) 478, 976, 1099, 1382
BspOI GCTAGC 1 cut(s) 1221
BspPI GGATC 3 cut(s) 12, 83, 330
BspQI GCTCTTC 2 cut(s) 723, 975
BspT104I TTCGAA 1 cut(s) 677
BspT107I GGYRCC 1 cut(s) 838
BsrDI GCAATG 1 cut(s) 1386
BsrGI TGTACA 1 cut(s) 255
BsrI ACTGG 2 cut(s) 139, 221
BssECI CCNNGG 1 cut(s) 1252
BssMI GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
BssNI GRCGYC 1 cut(s) 1340
BssT1I CCWWGG 1 cut(s) 1252
Bst4CI ACNGT 2 cut(s) 613, 790
Bst6I CTCTTC 4 cut(s) 474, 585, 723, 975
BstACI GRCGYC 1 cut(s) 1340
BstAPI GCANNNNNTGC 3 cut(s) 470, 749, 1175
BstAUI TGTACA 1 cut(s) 255
BstBI TTCGAA 1 cut(s) 677
BstC8I GCNNGC 1 cut(s) 1219
BstDEI CTNAG 8 cut(s) 195, 537, 849, 909, 927, 963, 1019, 1179
BstDSI CCRYGG 1 cut(s) 1252
BstF5I GGATG 3 cut(s) 313, 859, 1054
BstKTI GATC 8 cut(s) 7, 27, 46, 91, 325, 570, 1018, 1330
BstMAI GTCTC 5 cut(s) 10, 692, 779, 857, 1136
BstMBI GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
BstMWI GCNNNNNNNGC 5 cut(s) 470, 749, 1175, 1189, 1397
BstSFI CTRYAG 4 cut(s) 471, 612, 1092, 1146
BstV1I GCAGC 2 cut(s) 485, 1170
BstV2I GAAGAC 2 cut(s) 163, 1343
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 335
BtgI CCRYGG 1 cut(s) 1252
BtsCI GGATG 3 cut(s) 313, 859, 1054
BtsI GCAGTG 1 cut(s) 1081
BtsIMutI CAGTG 3 cut(s) 132, 945, 1081
BveI ACCTGC 4 cut(s) 478, 976, 1099, 1382
Cac8I GCNNGC 1 cut(s) 1219
CaiI CAGNNNCTG 1 cut(s) 1013
CseI GACGC 1 cut(s) 1348
Csp6I GTAC 2 cut(s) 256, 1161
CviAII CATG 6 cut(s) 47, 373, 640, 1253, 1294, 1420
CviQI GTAC 2 cut(s) 256, 1161
DdeI CTNAG 8 cut(s) 195, 537, 849, 909, 927, 963, 1019, 1179
DpnI GATC 8 cut(s) 6, 26, 45, 90, 324, 569, 1017, 1329
DpnII GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
DrdI GACNNNNNNGTC 1 cut(s) 706
DseDI GACNNNNNNGTC 1 cut(s) 706
Eam1104I CTCTTC 4 cut(s) 474, 585, 723, 975
EarI CTCTTC 4 cut(s) 474, 585, 723, 975
Ecl136II GAGCTC 1 cut(s) 967
Eco130I CCWWGG 1 cut(s) 1252
Eco24I GRGCYC 1 cut(s) 969
Eco53kI GAGCTC 1 cut(s) 967
Eco57I CTGAAG 1 cut(s) 498
Eco88I CYCGRG 1 cut(s) 888
EcoICRI GAGCTC 1 cut(s) 967
EcoT14I CCWWGG 1 cut(s) 1252
EcoT38I GRGCYC 1 cut(s) 969
ErhI CCWWGG 1 cut(s) 1252
Esp3I CGTCTC 1 cut(s) 779
FaeI CATG 6 cut(s) 50, 376, 643, 1256, 1297, 1423
FaqI GGGAC 1 cut(s) 205
FatI CATG 6 cut(s) 46, 372, 639, 1252, 1293, 1419
FauI CCCGC 1 cut(s) 1030
FbaI TGATCA 1 cut(s) 43
FblI GTMKAC 1 cut(s) 1063
Fnu4HI GCNGC 2 cut(s) 474, 1184
FokI GGATG 3 cut(s) 320, 866, 1061
FriOI GRGCYC 1 cut(s) 969
Fsp4HI GCNGC 2 cut(s) 474, 1184
FspBI CTAG 9 cut(s) 8, 446, 504, 669, 795, 1218, 1331, 1415, 1446
GluI GCNGC 2 cut(s) 474, 1184
GsuI CTGGAG 1 cut(s) 1064
HaeIII GGCC 1 cut(s) 335
HapII CCGG 1 cut(s) 305
HgaI GACGC 1 cut(s) 1348
Hin1I GRCGYC 1 cut(s) 1340
Hin1II CATG 6 cut(s) 50, 376, 643, 1256, 1297, 1423
HinfI GANTC 6 cut(s) 18, 193, 301, 327, 353, 1079
HpaII CCGG 1 cut(s) 305
HphI GGTGA 3 cut(s) 19, 247, 1334
Hpy166II GTNNAC 1 cut(s) 1064
Hpy188I TCNGA 8 cut(s) 69, 88, 198, 287, 300, 385, 567, 964
Hpy8I GTNNAC 1 cut(s) 1064
HpyAV CCTTC 6 cut(s) 133, 226, 628, 949, 1238, 1297
HpyCH4III ACNGT 2 cut(s) 613, 790
HpyCH4V TGCA 7 cut(s) 473, 694, 871, 991, 1094, 1186, 1391
HpyF10VI GCNNNNNNNGC 5 cut(s) 470, 749, 1175, 1189, 1397
HpyF3I CTNAG 8 cut(s) 195, 537, 849, 909, 927, 963, 1019, 1179
Hsp92I GRCGYC 1 cut(s) 1340
Hsp92II CATG 6 cut(s) 50, 376, 643, 1256, 1297, 1423
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
LguI GCTCTTC 2 cut(s) 723, 975
LmnI GCTCC 1 cut(s) 972
Lsp1109I GCAGC 2 cut(s) 485, 1170
LweI GCATC 1 cut(s) 844
MaeI CTAG 9 cut(s) 8, 446, 504, 669, 795, 1218, 1331, 1415, 1446
MaeIII GTNAC 3 cut(s) 823, 1076, 1227
MalI GATC 8 cut(s) 6, 26, 45, 90, 324, 569, 1017, 1329
MboI GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 2 cut(s) 969, 1376
MlyI GAGTC 3 cut(s) 12, 187, 1073
MroI TCCGGA 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 3 cut(s) 342, 1106, 1431
MslI CAYNNNNRTG 2 cut(s) 371, 1257
MspA1I CMGCKG 2 cut(s) 476, 1183
MspI CCGG 1 cut(s) 305
MwoI GCNNNNNNNGC 5 cut(s) 470, 749, 1175, 1189, 1397
NcoI CCATGG 1 cut(s) 1252
NdeII GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
NheI GCTAGC 1 cut(s) 1217
NlaIII CATG 6 cut(s) 50, 376, 643, 1256, 1297, 1423
NlaIV GGNNCC 3 cut(s) 486, 840, 1279
NmuCI GTSAC 1 cut(s) 1076
NspV TTCGAA 1 cut(s) 677
PaeR7I CTCGAG 1 cut(s) 888
PaqCI CACCTGC 1 cut(s) 976
PciSI GCTCTTC 2 cut(s) 723, 975
PcsI WCGNNNNNNNCGW 1 cut(s) 1063
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 3 cut(s) 301, 327, 353
PkrI GCNGC 2 cut(s) 475, 1185
PleI GAGTC 3 cut(s) 12, 187, 1073
PpsI GAGTC 3 cut(s) 12, 187, 1073
PsiI TTATAA 1 cut(s) 834
Psp124BI GAGCTC 1 cut(s) 969
PspN4I GGNNCC 3 cut(s) 486, 840, 1279
PspXI VCTCGAGB 1 cut(s) 888
PstI CTGCAG 2 cut(s) 475, 1096
PstNI CAGNNNCTG 1 cut(s) 1013
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 2 cut(s) 476, 1183
RsaI GTAC 2 cut(s) 257, 1162
RsaNI GTAC 2 cut(s) 256, 1161
RseI CAYNNNNRTG 2 cut(s) 371, 1257
SacI GAGCTC 1 cut(s) 969
SapI GCTCTTC 2 cut(s) 723, 975
SaqAI TTAA 3 cut(s) 342, 1106, 1431
SatI GCNGC 2 cut(s) 474, 1184
Sau3AI GATC 8 cut(s) 4, 24, 43, 88, 322, 567, 1015, 1327
SbfI CCTGCAGG 1 cut(s) 1096
SchI GAGTC 3 cut(s) 12, 187, 1073
SdaI CCTGCAGG 1 cut(s) 1096
SduI GDGCHC 2 cut(s) 969, 1376
SfaNI GCATC 1 cut(s) 844
SfcI CTRYAG 4 cut(s) 471, 612, 1092, 1146
Sfr274I CTCGAG 1 cut(s) 888
SfuI TTCGAA 1 cut(s) 677
SlaI CTCGAG 1 cut(s) 888
SmiMI CAYNNNNRTG 2 cut(s) 371, 1257
SmlI CTYRAG 4 cut(s) 20, 515, 888, 1125
SmoI CTYRAG 4 cut(s) 20, 515, 888, 1125
Sse8387I CCTGCAGG 1 cut(s) 1096
SsiI CCGC 1 cut(s) 1037
SspMI CTAG 9 cut(s) 8, 446, 504, 669, 795, 1218, 1331, 1415, 1446
SstI GAGCTC 1 cut(s) 969
StyI CCWWGG 1 cut(s) 1252
TaaI ACNGT 2 cut(s) 613, 790
TaqI TCGA 6 cut(s) 104, 325, 397, 677, 716, 889
TatI WGTACW 2 cut(s) 255, 1160
TfiI GAWTC 3 cut(s) 301, 327, 353
Tru1I TTAA 3 cut(s) 342, 1106, 1431
Tru9I TTAA 3 cut(s) 342, 1106, 1431
TscAI CASTG 3 cut(s) 139, 952, 1081
TseFI GTSAC 1 cut(s) 1076
TseI GCWGC 2 cut(s) 473, 1183
Tsp45I GTSAC 1 cut(s) 1076
TspDTI ATGAA 6 cut(s) 198, 603, 1275, 1299, 1310, 1372
TspRI CASTG 3 cut(s) 139, 952, 1081
XapI RAATTY 2 cut(s) 524, 1263
XbaI TCTAGA 2 cut(s) 7, 1330
XhoI CTCGAG 1 cut(s) 888
XmiI GTMKAC 1 cut(s) 1063
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 9 cut(s) 8, 446, 504, 669, 795, 1218, 1331, 1415, 1446
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.