Rmu_sc0015244.1_g000002

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0015244.1
OG0010913
Physical Location & Seq
Reverse (-)
1277 .. 5316
4040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0015244.1_g000002.1.cds

Sequence Viewer

Length: 1782 bp
atgttcttcttcctctgtttgctccctttactcttccagcattctcactccaccataatactagtggatggagtttcagagtggaagaaccctactgttcatgttggagactccatcattttcaagcacaagtatcactacaacctctacattttccagaaccaaagagccttcaatgtctgcaatttcactcgagccacacttcttaataaacccgattccacatcctatacgtggcacccatcacggcccggtttcttctacttctccttcaacaacggttctctgctcaatacatgccaagacacacaaaagcttgccataaaagttgctttaacaacaatggctccagtggcagcccctgaacccagttccaagacaggttctggtggcagtggtggtggtgtagtgtcatcttctccaacatatccatggcctttccaacctcaccaagcttccttatcgccaagtccagggccaagtgtcggctctttaattcctaataaaggtgctggcatgccatttatcaacagcaaccctgctgttcctttgcctactggtgaagtcgattctgctacaattcgccctataccaacatctggccaccagggacaggtgttggtggggttatttgctgcgcaaatggctctatttagtgtgaaagtaaaatctggagtttctaaggtgattcctagtcccctaaatccagaaatggatcttcaattaggactcaagatcaccaaaaatgcagatgatcatactacctcttctgattttcggattgcgaaggagaaatctagtcctgttttcctatccagcgaaacagaaagcgcgtacatcctcactgcccatcttaaaggttatagaaaagagcatgtcaatattgacataaatgaagacgagactcagctaggcattgctgtggagaagccagttcaagagaaggtgatgtcaggatggtcaatgaacaagaaagaaggggagacaaggggattcagaaaagtctttcggatcccagatggagtggttttgagtcgaatcaaggccaagattaatgaacaagactcgactctcagaatcttcatgccaaaagtggtgaaaggatatgttggtgttggggttggggttgaggaactgaaagaagaagaggtgatcatagggaaaccacagatgacagaagctgcaagtgatgaggttcctgaaaaaatcagtgagaaaatgaaaatggataagaaccctcaggctaaaggaaaagaggtagataaagaagagtacacgaaagaagttgaattagaggatcaagatttgcagaccaatgatcgaagagaaactgaaggtggagggatggagtcagaagaaagtgaagaacgaaagattgcaaagacaaaaaaagctgatagaattgagaaacagagagaggatgctaaaaaaagaatcaaggaaaaaacacatggaggagtcaagaatgataaaagcactgaatcaactcatcaagttgctaaaccatcacaagaaattgaagaaatcgaaccagaatcgagaccccccaacgcgaacgaaatgccaaaagcaacgccagagaagaaggaagtacttgacaagggtgaatcttctcaaatgcggaaaggaaaagatgtgtgtgagaaatcaactccagagaagaagcctgctgcgtcagaaaagtccaagttgctgtccactcctttgatcattgcaggttcagcccttctcgtcactattgtactgatagcaattcacaggattaggaaaagaaagcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

593

Amino Acids

65.54

Weight (kDa)

8.73

Isoelectric Point (pI)

46.46

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 639
Acc36I ACCTGC 1 cut(s) 1706
AccB1I GGYRCC 1 cut(s) 237
AccB7I CCANNNNNTGG 1 cut(s) 599
AccII CGCG 2 cut(s) 833, 1553
AciI CCGC 1 cut(s) 1621
AclWI GGATC 4 cut(s) 723, 1006, 1019, 1305
AcoI YGGCCR 1 cut(s) 601
AcuI CTGAAG 1 cut(s) 1353
AfaI GTAC 4 cut(s) 836, 1274, 1593, 1743
AfiI CCNNNNNNNGG 6 cut(s) 234, 473, 485, 506, 599, 613
AgsI TTSAA 7 cut(s) 124, 175, 274, 722, 938, 1289, 1520
AhlI ACTAGT 1 cut(s) 61
AjnI CCWGG 2 cut(s) 472, 606
AluBI AGCT 5 cut(s) 316, 455, 910, 1181, 1394
AluI AGCT 5 cut(s) 316, 455, 910, 1181, 1394
Alw26I GTCTC 4 cut(s) 102, 896, 977, 1534
AlwI GGATC 4 cut(s) 723, 1006, 1019, 1305
AlwNI CAGNNNCTG 3 cut(s) 362, 386, 1181
Ama87I CYCGRG 1 cut(s) 192
AoxI GGCC 5 cut(s) 248, 434, 476, 601, 1044
ApeKI GCWGC 4 cut(s) 356, 635, 1181, 1670
AseI ATTAAT 1 cut(s) 1053
AspLEI GCGC 2 cut(s) 640, 833
AspS9I GGNCC 2 cut(s) 249, 476
AsuC2I CCSGG 1 cut(s) 252
AsuHPI GGTGA 8 cut(s) 440, 572, 697, 730, 958, 1108, 1162, 1616
AvaI CYCGRG 1 cut(s) 192
AxyI CCTNAGG 1 cut(s) 1239
BalI TGGCCA 1 cut(s) 603
BamHI GGATCC 1 cut(s) 1011
BanI GGYRCC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 903
BbvI GCAGC 4 cut(s) 368, 622, 1168, 1657
BccI CCATC 8 cut(s) 62, 122, 250, 858, 951, 1013, 1339, 1513
BceAI ACGGC 1 cut(s) 263
BcgI CGANNNNNNTGC 2 cut(s) 1543, 1577
BciT130I CCWGG 2 cut(s) 474, 608
BclI TGATCA 3 cut(s) 754, 1152, 1707
BcnI CCSGG 1 cut(s) 252
BcoDI GTCTC 4 cut(s) 102, 896, 977, 1534
BcuI ACTAGT 1 cut(s) 61
BfaI CTAG 4 cut(s) 62, 693, 798, 911
BfuAI ACCTGC 1 cut(s) 1706
BisI GCNGC 4 cut(s) 357, 636, 1182, 1671
BlsI GCNGC 4 cut(s) 358, 637, 1183, 1672
BmcAI AGTACT 1 cut(s) 1593
Bme1390I CCNGG 3 cut(s) 252, 474, 608
BmeT110I CYCGRG 1 cut(s) 192
BmgT120I GGNCC 2 cut(s) 249, 476
BmiI GGNNCC 4 cut(s) 239, 348, 1013, 1197
BmrFI CCNGG 3 cut(s) 252, 474, 608
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 1411
BmuI ACTGGG 1 cut(s) 363
BpiI GAAGAC 1 cut(s) 903
BpmI CTGGAG 3 cut(s) 333, 693, 1638
BpuEI CTTGAG 1 cut(s) 716
BpuMI CCSGG 1 cut(s) 252
BsaAI YACGTR 1 cut(s) 234
BsaI GGTCTC 1 cut(s) 1534
BsaJI CCNNGG 3 cut(s) 431, 473, 607
BsaXI ACNNNNNCTCC 2 cut(s) 331, 361
Bsc4I CCNNNNNNNGG 6 cut(s) 234, 473, 485, 506, 599, 613
Bse1I ACTGG 4 cut(s) 350, 369, 562, 932
Bse21I CCTNAGG 1 cut(s) 1239
Bse3DI GCAATG 2 cut(s) 915, 1710
BseBI CCWGG 2 cut(s) 474, 608
BseDI CCNNGG 3 cut(s) 431, 473, 607
BseGI GGATG 6 cut(s) 73, 224, 837, 962, 1350, 1426
BseLI CCNNNNNNNGG 6 cut(s) 234, 473, 485, 506, 599, 613
BseMI GCAATG 2 cut(s) 915, 1710
BseMII CTCAG 3 cut(s) 920, 1087, 1253
BseNI ACTGG 4 cut(s) 350, 369, 562, 932
BseRI GAGGAG 1 cut(s) 1470
BseXI GCAGC 4 cut(s) 368, 622, 1168, 1657
Bsh1236I CGCG 2 cut(s) 833, 1553
BshFI GGCC 5 cut(s) 250, 436, 478, 603, 1046
BshNI GGYRCC 1 cut(s) 237
BsiHKCI CYCGRG 1 cut(s) 192
BsiSI CCGG 1 cut(s) 252
BslFI GGGAC 2 cut(s) 624, 681
BslI CCNNNNNNNGG 6 cut(s) 234, 473, 485, 506, 599, 613
BsmAI GTCTC 4 cut(s) 102, 896, 977, 1534
BsmFI GGGAC 2 cut(s) 624, 681
BsmI GAATGC 1 cut(s) 40
BsnI GGCC 5 cut(s) 250, 436, 478, 603, 1046
Bso31I GGTCTC 1 cut(s) 1534
BsoBI CYCGRG 1 cut(s) 192
Bsp143I GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
Bsp19I CCATGG 1 cut(s) 431
BspACI CCGC 1 cut(s) 1621
BspANI GGCC 5 cut(s) 250, 436, 478, 603, 1046
BspCNI CTCAG 3 cut(s) 919, 1086, 1252
BspFNI CGCG 2 cut(s) 833, 1553
BspLI GGNNCC 4 cut(s) 239, 348, 1013, 1197
BspMI ACCTGC 1 cut(s) 1706
BspPI GGATC 4 cut(s) 723, 1006, 1019, 1305
BspT107I GGYRCC 1 cut(s) 237
BspTNI GGTCTC 1 cut(s) 1534
BsrDI GCAATG 2 cut(s) 915, 1710
BsrI ACTGG 4 cut(s) 350, 369, 562, 932
BssECI CCNNGG 3 cut(s) 431, 473, 607
BssMI GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
BssT1I CCWWGG 1 cut(s) 431
Bst2UI CCWGG 2 cut(s) 474, 608
Bst4CI ACNGT 2 cut(s) 97, 281
Bst6I CTCTTC 5 cut(s) 38, 772, 1140, 1263, 1318
BstBAI YACGTR 1 cut(s) 234
BstC8I GCNNGC 4 cut(s) 318, 514, 518, 1668
BstDEI CTNAG 4 cut(s) 681, 906, 1073, 1239
BstDSI CCRYGG 1 cut(s) 431
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 6 cut(s) 73, 224, 837, 962, 1350, 1426
BstFNI CGCG 2 cut(s) 833, 1553
BstHHI GCGC 2 cut(s) 640, 833
BstKTI GATC 8 cut(s) 718, 738, 757, 1014, 1155, 1300, 1321, 1710
BstMAI GTCTC 4 cut(s) 102, 896, 977, 1534
BstMBI GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
BstMWI GCNNNNNNNGC 3 cut(s) 353, 644, 1721
BstNI CCWGG 2 cut(s) 474, 608
BstNSI RCATGY 3 cut(s) 300, 520, 878
BstSCI CCNGG 3 cut(s) 250, 472, 606
BstUI CGCG 2 cut(s) 833, 1553
BstV1I GCAGC 4 cut(s) 368, 622, 1168, 1657
BstV2I GAAGAC 1 cut(s) 903
BstX2I RGATCY 2 cut(s) 715, 1011
BstYI RGATCY 2 cut(s) 715, 1011
Bsu36I CCTNAGG 1 cut(s) 1239
BsuRI GGCC 5 cut(s) 250, 436, 478, 603, 1046
BtgI CCRYGG 1 cut(s) 431
BtsCI GGATG 6 cut(s) 73, 224, 837, 962, 1350, 1426
BtsI GCAGTG 2 cut(s) 400, 843
BtsIMutI CAGTG 5 cut(s) 357, 400, 843, 1216, 1476
BveI ACCTGC 1 cut(s) 1706
Cac8I GCNNGC 4 cut(s) 318, 514, 518, 1668
CaiI CAGNNNCTG 3 cut(s) 362, 386, 1181
CfoI GCGC 2 cut(s) 640, 833
Cfr13I GGNCC 2 cut(s) 249, 476
CseI GACGC 1 cut(s) 1662
Csp6I GTAC 4 cut(s) 835, 1273, 1592, 1742
CviAII CATG 7 cut(s) 101, 297, 432, 517, 875, 1084, 1451
CviQI GTAC 4 cut(s) 835, 1273, 1592, 1742
DdeI CTNAG 4 cut(s) 681, 906, 1073, 1239
DpnI GATC 8 cut(s) 717, 737, 756, 1013, 1154, 1299, 1320, 1709
DpnII GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
EaeI YGGCCR 1 cut(s) 601
Eam1104I CTCTTC 5 cut(s) 38, 772, 1140, 1263, 1318
EarI CTCTTC 5 cut(s) 38, 772, 1140, 1263, 1318
Eco130I CCWWGG 1 cut(s) 431
Eco31I GGTCTC 1 cut(s) 1534
Eco57I CTGAAG 1 cut(s) 1353
Eco81I CCTNAGG 1 cut(s) 1239
Eco88I CYCGRG 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoRII CCWGG 2 cut(s) 472, 606
EcoT14I CCWWGG 1 cut(s) 431
ErhI CCWWGG 1 cut(s) 431
FaeI CATG 7 cut(s) 104, 300, 435, 520, 878, 1087, 1454
FaqI GGGAC 2 cut(s) 624, 681
FatI CATG 7 cut(s) 100, 296, 431, 516, 874, 1083, 1450
FbaI TGATCA 3 cut(s) 754, 1152, 1707
Fnu4HI GCNGC 4 cut(s) 357, 636, 1182, 1671
FokI GGATG 6 cut(s) 80, 211, 824, 969, 1357, 1433
Fsp4HI GCNGC 4 cut(s) 357, 636, 1182, 1671
FspBI CTAG 4 cut(s) 62, 693, 798, 911
FspI TGCGCA 1 cut(s) 639
GlaI GCGC 2 cut(s) 639, 832
GluI GCNGC 4 cut(s) 357, 636, 1182, 1671
GsuI CTGGAG 3 cut(s) 333, 693, 1638
HaeIII GGCC 5 cut(s) 250, 436, 478, 603, 1046
HapII CCGG 1 cut(s) 252
HgaI GACGC 1 cut(s) 1662
HhaI GCGC 2 cut(s) 640, 833
Hin1II CATG 7 cut(s) 104, 300, 435, 520, 878, 1087, 1454
Hin6I GCGC 2 cut(s) 638, 831
HinP1I GCGC 2 cut(s) 638, 831
HindIII AAGCTT 2 cut(s) 314, 453
HpaII CCGG 1 cut(s) 252
HphI GGTGA 8 cut(s) 440, 572, 697, 730, 958, 1108, 1162, 1616
Hpy166II GTNNAC 2 cut(s) 1275, 1698
Hpy188I TCNGA 8 cut(s) 79, 772, 780, 998, 1011, 1076, 1354, 1678
Hpy8I GTNNAC 2 cut(s) 1275, 1698
HpyAV CCTTC 8 cut(s) 181, 280, 781, 937, 971, 1328, 1579, 1736
HpyCH4III ACNGT 2 cut(s) 97, 281
HpyCH4IV ACGT 1 cut(s) 233
HpyCH4V TGCA 6 cut(s) 183, 749, 1184, 1309, 1379, 1715
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 644, 1721
HpyF3I CTNAG 4 cut(s) 681, 906, 1073, 1239
HpySE526I ACGT 1 cut(s) 233
Hsp92II CATG 7 cut(s) 104, 300, 435, 520, 878, 1087, 1454
HspAI GCGC 2 cut(s) 638, 831
Ksp22I TGATCA 3 cut(s) 754, 1152, 1707
Kzo9I GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
LmnI GCTCC 2 cut(s) 27, 352
Lsp1109I GCAGC 4 cut(s) 368, 622, 1168, 1657
LweI GCATC 1 cut(s) 1411
MaeI CTAG 4 cut(s) 62, 693, 798, 911
MaeII ACGT 1 cut(s) 233
MaeIII GTNAC 1 cut(s) 1732
MalI GATC 8 cut(s) 717, 737, 756, 1013, 1154, 1299, 1320, 1709
MboI GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
MflI RGATCY 2 cut(s) 715, 1011
MlsI TGGCCA 1 cut(s) 603
MluCI AATT 8 cut(s) 184, 495, 579, 722, 1289, 1401, 1515, 1752
MluNI TGGCCA 1 cut(s) 603
MlyI GAGTC 8 cut(s) 104, 723, 898, 1042, 1058, 1063, 1358, 1467
MmeI TCCRAC 3 cut(s) 85, 446, 466
Mox20I TGGCCA 1 cut(s) 603
MscI TGGCCA 1 cut(s) 603
MseI TTAA 5 cut(s) 207, 335, 494, 855, 1053
MslI CAYNNNNRTG 2 cut(s) 430, 920
Msp20I TGGCCA 1 cut(s) 603
MspI CCGG 1 cut(s) 252
MspR9I CCNGG 3 cut(s) 252, 474, 608
Mva1269I GAATGC 1 cut(s) 40
MvaI CCWGG 2 cut(s) 474, 608
MvnI CGCG 2 cut(s) 833, 1553
MwoI GCNNNNNNNGC 3 cut(s) 353, 644, 1721
NciI CCSGG 1 cut(s) 252
NcoI CCATGG 1 cut(s) 431
NdeII GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
NlaIII CATG 7 cut(s) 104, 300, 435, 520, 878, 1087, 1454
NlaIV GGNNCC 4 cut(s) 239, 348, 1013, 1197
NmuCI GTSAC 1 cut(s) 1732
NsbI TGCGCA 1 cut(s) 639
NspI RCATGY 3 cut(s) 300, 520, 878
PaeI GCATGC 1 cut(s) 520
PaeR7I CTCGAG 1 cut(s) 192
PctI GAATGC 1 cut(s) 40
PflMI CCANNNNNTGG 1 cut(s) 599
PkrI GCNGC 4 cut(s) 358, 637, 1183, 1672
PleI GAGTC 8 cut(s) 104, 723, 898, 1041, 1058, 1063, 1357, 1466
PpsI GAGTC 8 cut(s) 104, 723, 898, 1041, 1058, 1063, 1357, 1466
Ppu21I YACGTR 1 cut(s) 234
PshBI ATTAAT 1 cut(s) 1053
Psp6I CCWGG 2 cut(s) 472, 606
PspGI CCWGG 2 cut(s) 472, 606
PspN4I GGNNCC 4 cut(s) 239, 348, 1013, 1197
PspPI GGNCC 2 cut(s) 249, 476
PspXI VCTCGAGB 1 cut(s) 192
PstNI CAGNNNCTG 3 cut(s) 362, 386, 1181
PsuI RGATCY 2 cut(s) 715, 1011
RsaI GTAC 4 cut(s) 836, 1274, 1593, 1743
RsaNI GTAC 4 cut(s) 835, 1273, 1592, 1742
RseI CAYNNNNRTG 2 cut(s) 430, 920
SaqAI TTAA 5 cut(s) 207, 335, 494, 855, 1053
SatI GCNGC 4 cut(s) 357, 636, 1182, 1671
Sau3AI GATC 8 cut(s) 715, 735, 754, 1011, 1152, 1297, 1318, 1707
Sau96I GGNCC 2 cut(s) 249, 476
ScaI AGTACT 1 cut(s) 1593
SchI GAGTC 8 cut(s) 104, 723, 898, 1042, 1058, 1063, 1358, 1467
ScrFI CCNGG 3 cut(s) 252, 474, 608
SfaNI GCATC 1 cut(s) 1411
Sfr274I CTCGAG 1 cut(s) 192
SlaI CTCGAG 1 cut(s) 192
SmiMI CAYNNNNRTG 2 cut(s) 430, 920
SmlI CTYRAG 2 cut(s) 192, 731
SmoI CTYRAG 2 cut(s) 192, 731
SpeI ACTAGT 1 cut(s) 61
SphI GCATGC 1 cut(s) 520
Sse9I AATT 8 cut(s) 184, 495, 579, 722, 1289, 1401, 1515, 1752
SsiI CCGC 1 cut(s) 1621
SspI AATATT 1 cut(s) 883
SspMI CTAG 4 cut(s) 62, 693, 798, 911
StyD4I CCNGG 3 cut(s) 250, 472, 606
StyI CCWWGG 1 cut(s) 431
TaaI ACNGT 2 cut(s) 97, 281
TaiI ACGT 1 cut(s) 236
TaqI TCGA 7 cut(s) 193, 567, 1036, 1067, 1321, 1527, 1538
TasI AATT 8 cut(s) 184, 495, 579, 722, 1289, 1401, 1515, 1752
TatI WGTACW 3 cut(s) 1272, 1591, 1741
Tru1I TTAA 5 cut(s) 207, 335, 494, 855, 1053
Tru9I TTAA 5 cut(s) 207, 335, 494, 855, 1053
TscAI CASTG 5 cut(s) 357, 400, 850, 1216, 1483
TseFI GTSAC 1 cut(s) 1732
TseI GCWGC 4 cut(s) 356, 635, 1181, 1670
Tsp45I GTSAC 1 cut(s) 1732
TspDTI ATGAA 6 cut(s) 89, 909, 980, 1071, 1072, 1235
TspRI CASTG 5 cut(s) 357, 400, 850, 1216, 1483
Van91I CCANNNNNTGG 1 cut(s) 599
VspI ATTAAT 1 cut(s) 1053
XagI CCTNNNNNAGG 1 cut(s) 504
XceI RCATGY 3 cut(s) 300, 520, 878
XcmI CCANNNNNNNNNTGG 2 cut(s) 61, 429
XhoI CTCGAG 1 cut(s) 192
XspI CTAG 4 cut(s) 62, 693, 798, 911
ZrmI AGTACT 1 cut(s) 1593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.