RchiOBHm_Chr6g0275301

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0010913
Physical Location & Seq
Forward (+)
37068934 .. 37070425
1492 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24699

Sequence Viewer

Length: 1074 bp
ATGGATCTTGAATTAGGACTCGAGATCACCAAAAATGCAGATGATCATACTTCCTCTTCTGATTTTCGGATTGCGAAGGAGAAATCTGGTCCTGTTTTCATATCCACCGAAACAGAAAGCGCGTACATCCTCACTGCCCATCTTGAAGGTTGTAGAAAAGAGCATGTCAATATTGACATAAATGAAGACGAGACTCAGCTAGGCGTTGCTGTGGAGAAGCCAGTTCAAGAGAAGGTGATGTCAGGATGGTCAATGAACAAGAAAGAAGGGGAGACAAGGGGATTCAGAAAAGTCTTTCGGATCCCAGATGGAGTGGTTTTGAGTCGAATCAAGGCCAAGATTAATGAACAAGACTCGACTCTCAGAATCTTCATGCCAAAAGTGGTGAAAGGATTTGTTGGTGCTGGGGTTGAGGAACTGAAAGAAGAAGAGGTGATCATAGGGCAACCACAGATGACAGAAGCTGCAAGTGATGAGGTTCCTGAAAAAATCAGTGAGAAAATGAAAATGGATAAGAACCCTCAGGCTGAAGGAAAAGAGGTAGATAAAGAAGAGTACACGAAAGAAGTTGAATTAGATGATCAAGATTTGCAGACCAATGATCGAAGAGAAACTAAAGGTGGAGGGATGGAGTCAGAAGAAAGTGAAGAAAGTGAAGAAAGAAAGATTGCAAAGACAAAAAAAGCTGATAGAATTGAGAAACAGAGAGAGGATGCTAAAAAAAGAATCAAGGAAAAAACACATGGAGGAGTCAAGAATGATAAAAGCACTGAAGCAACTCATCAAGTTGCTAAACCATCACAAGAAATTGAAGAAATCAGACCAGAATCGAGACCCCCCAACGCGAACGAAATGCCAAAAGCGACGCCAGAGAAGAAGGAAGTACTTGACAAGGGTGAATCTTCTGAAATGCGGAAAGGAAAAGATGTGTGTGAGAAATCAACTCCAGAGAAGAAGCCTGCTGCGTCAGAAAAGTCCAAGTTGCTGTCCACTCCTTTGATCATTGCAGGTTCAGCCCTTCTCGTCACTATTGTACTGATAGCAATTCACAGGATTAGGAAAAGAAAGCAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

357

Amino Acids

40.12

Weight (kDa)

5.71

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 998
AccII CGCG 2 cut(s) 122, 845
AciI CCGC 1 cut(s) 913
AclWI GGATC 3 cut(s) 12, 295, 308
AcuI CTGAAG 2 cut(s) 549, 792
AcyI GRCGYC 1 cut(s) 866
AfaI GTAC 4 cut(s) 125, 557, 885, 1035
AgsI TTSAA 5 cut(s) 11, 146, 227, 572, 812
AluBI AGCT 3 cut(s) 199, 464, 686
AluI AGCT 3 cut(s) 199, 464, 686
Alw26I GTCTC 3 cut(s) 185, 266, 826
AlwI GGATC 3 cut(s) 12, 295, 308
AlwNI CAGNNNCTG 1 cut(s) 464
Ama87I CYCGRG 1 cut(s) 20
AoxI GGCC 1 cut(s) 333
ApeKI GCWGC 2 cut(s) 464, 962
AseI ATTAAT 1 cut(s) 342
AspLEI GCGC 1 cut(s) 122
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 19, 247, 397, 445, 908
AvaI CYCGRG 1 cut(s) 20
AvaII GGWCC 1 cut(s) 89
AxyI CCTNAGG 1 cut(s) 522
BamHI GGATCC 1 cut(s) 300
BbsI GAAGAC 1 cut(s) 192
BbvI GCAGC 2 cut(s) 451, 949
BccI CCATC 5 cut(s) 147, 240, 302, 622, 805
BcgI CGANNNNNNTGC 2 cut(s) 835, 869
BclI TGATCA 4 cut(s) 43, 435, 580, 999
BcoDI GTCTC 3 cut(s) 185, 266, 826
BfaI CTAG 1 cut(s) 200
BfuAI ACCTGC 1 cut(s) 998
BisI GCNGC 2 cut(s) 465, 963
BlsI GCNGC 2 cut(s) 466, 964
BmcAI AGTACT 1 cut(s) 885
Bme18I GGWCC 1 cut(s) 89
BmeT110I CYCGRG 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 302, 480
BmsI GCATC 1 cut(s) 703
BpiI GAAGAC 1 cut(s) 192
BpmI CTGGAG 1 cut(s) 930
BsaHI GRCGYC 1 cut(s) 866
BsaI GGTCTC 1 cut(s) 826
Bse1I ACTGG 1 cut(s) 221
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 1002
BseGI GGATG 4 cut(s) 126, 251, 633, 718
BseMI GCAATG 1 cut(s) 1002
BseMII CTCAG 3 cut(s) 209, 376, 536
BseNI ACTGG 1 cut(s) 221
BseRI GAGGAG 1 cut(s) 762
BseXI GCAGC 2 cut(s) 451, 949
BseYI CCCAGC 1 cut(s) 404
Bsh1236I CGCG 2 cut(s) 122, 845
BshFI GGCC 1 cut(s) 335
BsiHKCI CYCGRG 1 cut(s) 20
BsmAI GTCTC 3 cut(s) 185, 266, 826
BsnI GGCC 1 cut(s) 335
Bso31I GGTCTC 1 cut(s) 826
BsoBI CYCGRG 1 cut(s) 20
Bsp143I GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
BspACI CCGC 1 cut(s) 913
BspANI GGCC 1 cut(s) 335
BspCNI CTCAG 3 cut(s) 208, 375, 535
BspFNI CGCG 2 cut(s) 122, 845
BspLI GGNNCC 2 cut(s) 302, 480
BspMI ACCTGC 1 cut(s) 998
BspPI GGATC 3 cut(s) 12, 295, 308
BspTNI GGTCTC 1 cut(s) 826
BsrDI GCAATG 1 cut(s) 1002
BsrI ACTGG 1 cut(s) 221
BssMI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
BssNI GRCGYC 1 cut(s) 866
Bst6I CTCTTC 4 cut(s) 61, 423, 546, 601
BstACI GRCGYC 1 cut(s) 866
BstC8I GCNNGC 1 cut(s) 960
BstDEI CTNAG 3 cut(s) 195, 362, 522
BstF5I GGATG 4 cut(s) 126, 251, 633, 718
BstFNI CGCG 2 cut(s) 122, 845
BstHHI GCGC 1 cut(s) 122
BstKTI GATC 8 cut(s) 7, 27, 46, 303, 438, 583, 604, 1002
BstMAI GTCTC 3 cut(s) 185, 266, 826
BstMBI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
BstMWI GCNNNNNNNGC 1 cut(s) 1013
BstNSI RCATGY 1 cut(s) 167
BstUI CGCG 2 cut(s) 122, 845
BstV1I GCAGC 2 cut(s) 451, 949
BstV2I GAAGAC 1 cut(s) 192
BstX2I RGATCY 2 cut(s) 4, 300
BstYI RGATCY 2 cut(s) 4, 300
Bsu36I CCTNAGG 1 cut(s) 522
BsuRI GGCC 1 cut(s) 335
BtsCI GGATG 4 cut(s) 126, 251, 633, 718
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 3 cut(s) 132, 499, 768
BveI ACCTGC 1 cut(s) 998
Cac8I GCNNGC 1 cut(s) 960
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 1 cut(s) 122
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 2 cut(s) 874, 954
Csp6I GTAC 4 cut(s) 124, 556, 884, 1034
CviAII CATG 3 cut(s) 164, 373, 743
CviJI RGCY 8 cut(s) 199, 220, 335, 464, 527, 686, 958, 1016
CviKI_1 RGCY 8 cut(s) 199, 220, 335, 464, 527, 686, 958, 1016
CviQI GTAC 4 cut(s) 124, 556, 884, 1034
DdeI CTNAG 3 cut(s) 195, 362, 522
DpnI GATC 8 cut(s) 6, 26, 45, 302, 437, 582, 603, 1001
DpnII GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
Eam1104I CTCTTC 4 cut(s) 61, 423, 546, 601
EarI CTCTTC 4 cut(s) 61, 423, 546, 601
Eco31I GGTCTC 1 cut(s) 826
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 2 cut(s) 549, 792
Eco81I CCTNAGG 1 cut(s) 522
Eco88I CYCGRG 1 cut(s) 20
FaeI CATG 3 cut(s) 167, 376, 746
FaiI YATR 7 cut(s) 48, 101, 165, 179, 374, 440, 744
FatI CATG 3 cut(s) 163, 372, 742
FbaI TGATCA 4 cut(s) 43, 435, 580, 999
Fnu4HI GCNGC 2 cut(s) 465, 963
FokI GGATG 4 cut(s) 113, 258, 640, 725
Fsp4HI GCNGC 2 cut(s) 465, 963
FspBI CTAG 1 cut(s) 200
GlaI GCGC 1 cut(s) 121
GluI GCNGC 2 cut(s) 465, 963
GsaI CCCAGC 1 cut(s) 408
GsuI CTGGAG 1 cut(s) 930
HaeIII GGCC 1 cut(s) 335
HgaI GACGC 2 cut(s) 874, 954
HhaI GCGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 866
Hin1II CATG 3 cut(s) 167, 376, 746
Hin6I GCGC 1 cut(s) 120
HinP1I GCGC 1 cut(s) 120
HphI GGTGA 5 cut(s) 19, 247, 397, 445, 908
Hpy166II GTNNAC 2 cut(s) 558, 990
Hpy188I TCNGA 9 cut(s) 61, 69, 287, 300, 365, 637, 821, 907, 970
Hpy8I GTNNAC 2 cut(s) 558, 990
Hpy99I CGWCG 1 cut(s) 868
HpyAV CCTTC 7 cut(s) 70, 140, 226, 260, 524, 871, 1028
HpyCH4V TGCA 5 cut(s) 38, 467, 592, 671, 1007
HpyF10VI GCNNNNNNNGC 1 cut(s) 1013
HpyF3I CTNAG 3 cut(s) 195, 362, 522
Hsp92I GRCGYC 1 cut(s) 866
Hsp92II CATG 3 cut(s) 167, 376, 746
HspAI GCGC 1 cut(s) 120
Ksp22I TGATCA 4 cut(s) 43, 435, 580, 999
Kzo9I GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
Lsp1109I GCAGC 2 cut(s) 451, 949
LweI GCATC 1 cut(s) 703
MaeI CTAG 1 cut(s) 200
MaeIII GTNAC 1 cut(s) 1024
MalI GATC 8 cut(s) 6, 26, 45, 302, 437, 582, 603, 1001
MboI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
MflI RGATCY 2 cut(s) 4, 300
MluCI AATT 5 cut(s) 11, 572, 693, 807, 1044
MlyI GAGTC 7 cut(s) 12, 187, 331, 347, 352, 641, 759
MseI TTAA 1 cut(s) 342
MvnI CGCG 2 cut(s) 122, 845
MwoI GCNNNNNNNGC 1 cut(s) 1013
NdeII GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
NlaIII CATG 3 cut(s) 167, 376, 746
NlaIV GGNNCC 2 cut(s) 302, 480
NmuCI GTSAC 1 cut(s) 1024
NspI RCATGY 1 cut(s) 167
PaeR7I CTCGAG 1 cut(s) 20
PfeI GAWTC 6 cut(s) 282, 327, 366, 726, 827, 899
PkrI GCNGC 2 cut(s) 466, 964
PleI GAGTC 7 cut(s) 12, 187, 330, 347, 352, 640, 758
PpsI GAGTC 7 cut(s) 12, 187, 330, 347, 352, 640, 758
PshBI ATTAAT 1 cut(s) 342
PspFI CCCAGC 1 cut(s) 404
PspN4I GGNNCC 2 cut(s) 302, 480
PspPI GGNCC 1 cut(s) 89
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 4, 300
RsaI GTAC 4 cut(s) 125, 557, 885, 1035
RsaNI GTAC 4 cut(s) 124, 556, 884, 1034
SaqAI TTAA 1 cut(s) 342
SatI GCNGC 2 cut(s) 465, 963
Sau3AI GATC 8 cut(s) 4, 24, 43, 300, 435, 580, 601, 999
Sau96I GGNCC 1 cut(s) 89
ScaI AGTACT 1 cut(s) 885
SchI GAGTC 7 cut(s) 12, 187, 331, 347, 352, 641, 759
SfaNI GCATC 1 cut(s) 703
Sfr274I CTCGAG 1 cut(s) 20
SinI GGWCC 1 cut(s) 89
SlaI CTCGAG 1 cut(s) 20
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 5 cut(s) 11, 572, 693, 807, 1044
SsiI CCGC 1 cut(s) 913
SspI AATATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 200
TaqI TCGA 5 cut(s) 21, 325, 356, 604, 830
TasI AATT 5 cut(s) 11, 572, 693, 807, 1044
TatI WGTACW 3 cut(s) 555, 883, 1033
TfiI GAWTC 6 cut(s) 282, 327, 366, 726, 827, 899
Tru1I TTAA 1 cut(s) 342
Tru9I TTAA 1 cut(s) 342
TscAI CASTG 3 cut(s) 139, 499, 775
TseFI GTSAC 1 cut(s) 1024
TseI GCWGC 2 cut(s) 464, 962
Tsp45I GTSAC 1 cut(s) 1024
TspDTI ATGAA 6 cut(s) 88, 198, 269, 360, 361, 518
TspRI CASTG 3 cut(s) 139, 499, 775
VpaK11BI GGWCC 1 cut(s) 89
VspI ATTAAT 1 cut(s) 342
XceI RCATGY 1 cut(s) 167
XhoI CTCGAG 1 cut(s) 20
XspI CTAG 1 cut(s) 200
ZrmI AGTACT 1 cut(s) 885
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.