pycom10g01750

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0010913
Physical Location & Seq
Reverse (-)
1849203 .. 1850703
1501 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g01750.3

Sequence Viewer

Length: 1353 bp
ATGGATCTAGAATTAGGACTCAAGATCGCCCACAGCGTAGACGATCATGTTTCGTCTACCAACTTTTGGATTGCCAAGGAGAAATCCGGTCCTATTTTCGTGTCTAAGGAAACCGAAACCATGTTCATCCTCACTGCCCATCTCAATGGTTATAGAAGAGAGCGTGTATATATCGACATAAACGAAGATGGGACTCAGATTGGAATCAGTGGGGAGAAGCCAGTTCAAGAGAAGGTTATGTCAGGATGGATAATGTACAAGAAAGAGGTGGAGATGAGGGGTTTCCGAAAAGTCTTTCAAATTCCGGATGGAGTGGTTTTGGATCGAATCATAGGAATTCGTGGAGTTGAGATCGAGGAAGTGAAAGAAGATGAAGTGGATATAGGGAGACAGCAGCTCACAAAACCTCCTGCGGCTGGAGAAGTCCCTGAAAATGATGGTTTTAGGGGGAAAATGGAGGAACAATTTGGAGAGCCTGAGAAAAAGAAAGTAGGAGAAAATTGTCGAACTTATGCGAAAGGGGTTGATGAAGAGGGCTCCAAGAAAGAAATTGTAGCTGATGGAGTTATCGAAAAGGATGCGAGCAGAGGCGTAGTGCAGGAAGAAGCTAAAGATTCAAAGACTCAAAGTATGCAACACCAAACAAAATCTCTTTCGGAACACAGTGAAGAGCTAAAAGTTGCAAAGGTTAAAGAAGCTGATGGAGTTGAGAAAGAGAAAGTTGGAAGAAGGGAACTCGGAAGAGACCAAATTGTAGGGGACAAGGTTCCAAGGATCAGCGGGGATGCTGAAGAGCCAATGAATGTGGATAAAACCACTGAAGTAACTCATGAAGTTACTCGGTCATCACAAGAAACTCAGGAAATTGCTAAGCATGAAACAGCTGAAGAAAACTCTACTGCAGAGCCCGAATTAGAAACGCCAAAGGCGGGGATCGAGGAACACGTTAGAAGTAGACCAGAGAGCCGCGAATCATCAAAACTTGCACAAGACAGAAGAGCAACACAAGAAGAAGCACAAGTTGGACACGAAAAGAATACGGATGGAGTTAGTAAAGAAGGCGATATTGTTCAGCAGCTACCAAAGCAAGAATCCATCGAAAAAGGTCACGAAAACGAAATCGAAGAAGTATCAAAGAATGAAGAACAAAATCAAGAGAAGGAAGAGGATGAATCTTCCGAAACGGAGGAACAAAGTGATGTGGCTGATGAGGAATCAAGTGAGGATATGGGGAAGAAGGATGGTTTGGAAAAGTCTAATCTGCTGTGCTCTCCTTTTATCATTGCAGGTTCAGCCCTTATTGTGTCCCTGCTGGTGCTAGCAATCAACAGGATTAGAACCAGAAAAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

451

Amino Acids

50.41

Weight (kDa)

4.83

Isoelectric Point (pI)

48.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1277
AccB7I CCANNNNNTGG 1 cut(s) 66
AccI GTMKAC 3 cut(s) 39, 56, 955
AccII CGCG 1 cut(s) 969
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 4 cut(s) 413, 780, 929, 967
AclWI GGATC 4 cut(s) 12, 330, 782, 941
AcsI RAATTY 2 cut(s) 300, 336
AcuI CTGAAG 3 cut(s) 810, 840, 906
AfaI GTAC 1 cut(s) 257
AfiI CCNNNNNNNGG 3 cut(s) 66, 416, 929
AflIII ACRYGT 1 cut(s) 943
AgsI TTSAA 3 cut(s) 227, 299, 618
AjuI GAANNNNNNNTTGG 4 cut(s) 1005, 1037, 1229, 1261
AluBI AGCT 7 cut(s) 397, 557, 608, 673, 698, 884, 1078
AluI AGCT 7 cut(s) 397, 557, 608, 673, 698, 884, 1078
Alw21I GWGCWC 1 cut(s) 1271
Alw26I GTCTC 2 cut(s) 382, 738
AlwI GGATC 4 cut(s) 12, 330, 782, 941
Aor13HI TCCGGA 1 cut(s) 304
ApeKI GCWGC 2 cut(s) 394, 1075
ApoI RAATTY 2 cut(s) 300, 336
AspS9I GGNCC 1 cut(s) 89
AsuNHI GCTAGC 1 cut(s) 1318
AvaII GGWCC 1 cut(s) 89
BanII GRGCYC 2 cut(s) 539, 909
Bbv12I GWGCWC 1 cut(s) 1271
BbvI GCAGC 2 cut(s) 406, 1087
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BcoDI GTCTC 2 cut(s) 382, 738
BfaI CTAG 2 cut(s) 8, 1319
BfmI CTRYAG 1 cut(s) 900
BfuAI ACCTGC 1 cut(s) 1277
BisI GCNGC 4 cut(s) 395, 414, 967, 1076
BlpI GCTNAGC 1 cut(s) 870
BlsI GCNGC 4 cut(s) 396, 415, 968, 1077
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmiI GGNNCC 2 cut(s) 538, 768
BmsI GCATC 2 cut(s) 568, 775
BmtI GCTAGC 1 cut(s) 1322
BpmI CTGGAG 1 cut(s) 438
Bpu1102I GCTNAGC 1 cut(s) 870
BpuEI CTTGAG 1 cut(s) 5
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 738
BsaJI CCNNGG 2 cut(s) 75, 770
BsaWI WCCGGW 2 cut(s) 86, 304
BsaXI ACNNNNNCTCC 2 cut(s) 391, 421
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 416, 929
Bse1I ACTGG 1 cut(s) 221
Bse3DI GCAATG 1 cut(s) 1281
Bse8I GATNNNNATC 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 75, 770
BseGI GGATG 8 cut(s) 126, 251, 313, 583, 790, 1048, 1174, 1246
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 3 cut(s) 66, 416, 929
BseMI GCAATG 1 cut(s) 1281
BseMII CTCAG 3 cut(s) 209, 468, 872
BseNI ACTGG 1 cut(s) 221
BseXI GCAGC 2 cut(s) 406, 1087
BsgI GTGCAG 1 cut(s) 617
Bsh1236I CGCG 1 cut(s) 969
BsiHKAI GWGCWC 1 cut(s) 1271
BsiSI CCGG 2 cut(s) 87, 305
BslFI GGGAC 4 cut(s) 205, 410, 773, 1291
BslI CCNNNNNNNGG 3 cut(s) 66, 416, 929
BsmAI GTCTC 2 cut(s) 382, 738
BsmFI GGGAC 4 cut(s) 205, 410, 773, 1291
Bso31I GGTCTC 1 cut(s) 738
Bsp1286I GDGCHC 3 cut(s) 539, 909, 1271
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 1 cut(s) 255
Bsp143I GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
Bsp1720I GCTNAGC 1 cut(s) 870
BspACI CCGC 4 cut(s) 413, 780, 929, 967
BspCNI CTCAG 3 cut(s) 208, 469, 871
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 1 cut(s) 969
BspHI TCATGA 1 cut(s) 829
BspLI GGNNCC 2 cut(s) 538, 768
BspMAI CTGCAG 1 cut(s) 904
BspMI ACCTGC 1 cut(s) 1277
BspOI GCTAGC 1 cut(s) 1322
BspPI GGATC 4 cut(s) 12, 330, 782, 941
BspQI GCTCTTC 3 cut(s) 663, 786, 991
BspTNI GGTCTC 1 cut(s) 738
BsrDI GCAATG 1 cut(s) 1281
BsrGI TGTACA 1 cut(s) 255
BsrI ACTGG 1 cut(s) 221
BssECI CCNNGG 2 cut(s) 75, 770
BssMI GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
BssT1I CCWWGG 2 cut(s) 75, 770
Bst4CI ACNGT 1 cut(s) 665
Bst6I CTCTTC 7 cut(s) 151, 525, 663, 736, 786, 991, 1158
BstAUI TGTACA 1 cut(s) 255
BstC8I GCNNGC 2 cut(s) 583, 1320
BstDEI CTNAG 5 cut(s) 105, 195, 477, 858, 870
BstF5I GGATG 8 cut(s) 126, 251, 313, 583, 790, 1048, 1174, 1246
BstFNI CGCG 1 cut(s) 969
BstKTI GATC 7 cut(s) 7, 27, 46, 325, 354, 777, 936
BstMAI GTCTC 2 cut(s) 382, 738
BstMBI GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
BstMWI GCNNNNNNNGC 2 cut(s) 1084, 1292
BstSFI CTRYAG 1 cut(s) 900
BstUI CGCG 1 cut(s) 969
BstV1I GCAGC 2 cut(s) 406, 1087
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 146
BstYI RGATCY 1 cut(s) 4
BtsCI GGATG 8 cut(s) 126, 251, 313, 583, 790, 1048, 1174, 1246
BtsI GCAGTG 1 cut(s) 132
BtsIMutI CAGTG 4 cut(s) 132, 214, 670, 816
BveI ACCTGC 1 cut(s) 1277
Cac8I GCNNGC 2 cut(s) 583, 1320
CciI TCATGA 1 cut(s) 829
Cfr13I GGNCC 1 cut(s) 89
Csp6I GTAC 1 cut(s) 256
CspCI CAANNNNNGTGG 2 cut(s) 786, 821
CviAII CATG 4 cut(s) 47, 121, 830, 875
CviQI GTAC 1 cut(s) 256
DdeI CTNAG 5 cut(s) 105, 195, 477, 858, 870
DpnI GATC 7 cut(s) 6, 26, 45, 324, 353, 776, 935
DpnII GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
Eam1104I CTCTTC 7 cut(s) 151, 525, 663, 736, 786, 991, 1158
EarI CTCTTC 7 cut(s) 151, 525, 663, 736, 786, 991, 1158
Eco130I CCWWGG 2 cut(s) 75, 770
Eco24I GRGCYC 2 cut(s) 539, 909
Eco31I GGTCTC 1 cut(s) 738
Eco47I GGWCC 1 cut(s) 89
Eco57I CTGAAG 3 cut(s) 810, 840, 906
EcoRI GAATTC 1 cut(s) 336
EcoT14I CCWWGG 2 cut(s) 75, 770
EcoT38I GRGCYC 2 cut(s) 539, 909
ErhI CCWWGG 2 cut(s) 75, 770
FaeI CATG 4 cut(s) 50, 124, 833, 878
FaqI GGGAC 4 cut(s) 205, 410, 773, 1291
FatI CATG 4 cut(s) 46, 120, 829, 874
FauI CCCGC 2 cut(s) 773, 922
FblI GTMKAC 3 cut(s) 39, 56, 955
Fnu4HI GCNGC 4 cut(s) 395, 414, 967, 1076
FokI GGATG 8 cut(s) 113, 258, 320, 590, 797, 1055, 1181, 1253
FriOI GRGCYC 2 cut(s) 539, 909
Fsp4HI GCNGC 4 cut(s) 395, 414, 967, 1076
FspBI CTAG 2 cut(s) 8, 1319
GluI GCNGC 4 cut(s) 395, 414, 967, 1076
GsuI CTGGAG 1 cut(s) 438
HapII CCGG 2 cut(s) 87, 305
Hin1II CATG 4 cut(s) 50, 124, 833, 878
HpaII CCGG 2 cut(s) 87, 305
Hpy166II GTNNAC 3 cut(s) 40, 57, 956
Hpy188I TCNGA 5 cut(s) 198, 287, 658, 740, 1180
Hpy188III TCNNGA 9 cut(s) 8, 22, 227, 243, 305, 830, 860, 1109, 1154
Hpy8I GTNNAC 3 cut(s) 40, 57, 956
HpyAV CCTTC 5 cut(s) 226, 723, 1052, 1153, 1231
HpyCH4III ACNGT 1 cut(s) 665
HpyCH4IV ACGT 1 cut(s) 945
HpyCH4V TGCA 6 cut(s) 598, 634, 683, 902, 986, 1286
HpyF10VI GCNNNNNNNGC 2 cut(s) 1084, 1292
HpyF3I CTNAG 5 cut(s) 105, 195, 477, 858, 870
HpySE526I ACGT 1 cut(s) 945
Hsp92II CATG 4 cut(s) 50, 124, 833, 878
Kpn2I TCCGGA 1 cut(s) 304
Kzo9I GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
LguI GCTCTTC 3 cut(s) 663, 786, 991
LmnI GCTCC 1 cut(s) 542
Lsp1109I GCAGC 2 cut(s) 406, 1087
LweI GCATC 2 cut(s) 568, 775
MaeI CTAG 2 cut(s) 8, 1319
MaeII ACGT 1 cut(s) 945
MaeIII GTNAC 3 cut(s) 823, 835, 1106
MalI GATC 7 cut(s) 6, 26, 45, 324, 353, 776, 935
MboI GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 539, 909, 1271
MluCI AATT 9 cut(s) 11, 300, 336, 464, 499, 549, 750, 864, 911
MlyI GAGTC 3 cut(s) 12, 187, 616
MmeI TCCRAC 2 cut(s) 703, 1003
MroI TCCGGA 1 cut(s) 304
MseI TTAA 1 cut(s) 690
MslI CAYNNNNRTG 1 cut(s) 144
MspA1I CMGCKG 2 cut(s) 780, 884
MspI CCGG 2 cut(s) 87, 305
MvnI CGCG 1 cut(s) 969
MwoI GCNNNNNNNGC 2 cut(s) 1084, 1292
NdeII GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
NheI GCTAGC 1 cut(s) 1318
NlaIII CATG 4 cut(s) 50, 124, 833, 878
NlaIV GGNNCC 2 cut(s) 538, 768
NmuCI GTSAC 1 cut(s) 1106
PagI TCATGA 1 cut(s) 829
PciSI GCTCTTC 3 cut(s) 663, 786, 991
PcsI WCGNNNNNNNCGW 3 cut(s) 33, 180, 942
PfeI GAWTC 7 cut(s) 204, 327, 614, 971, 1091, 1172, 1214
PflMI CCANNNNNTGG 1 cut(s) 66
PkrI GCNGC 4 cut(s) 396, 415, 968, 1077
PleI GAGTC 3 cut(s) 12, 187, 616
PpsI GAGTC 3 cut(s) 12, 187, 616
PspN4I GGNNCC 2 cut(s) 538, 768
PspPI GGNCC 1 cut(s) 89
PstI CTGCAG 1 cut(s) 904
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 884
RsaI GTAC 1 cut(s) 257
RsaNI GTAC 1 cut(s) 256
RseI CAYNNNNRTG 1 cut(s) 144
SapI GCTCTTC 3 cut(s) 663, 786, 991
SaqAI TTAA 1 cut(s) 690
SatI GCNGC 4 cut(s) 395, 414, 967, 1076
Sau3AI GATC 7 cut(s) 4, 24, 43, 322, 351, 774, 933
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 3 cut(s) 12, 187, 616
SduI GDGCHC 3 cut(s) 539, 909, 1271
SfaNI GCATC 2 cut(s) 568, 775
SfcI CTRYAG 1 cut(s) 900
SinI GGWCC 1 cut(s) 89
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 9 cut(s) 11, 300, 336, 464, 499, 549, 750, 864, 911
SsiI CCGC 4 cut(s) 413, 780, 929, 967
SspMI CTAG 2 cut(s) 8, 1319
StyI CCWWGG 2 cut(s) 75, 770
TaaI ACNGT 1 cut(s) 665
TaiI ACGT 1 cut(s) 948
TaqI TCGA 8 cut(s) 174, 325, 354, 505, 570, 936, 1098, 1122
TaqII GACCGA 1 cut(s) 831
TasI AATT 9 cut(s) 11, 300, 336, 464, 499, 549, 750, 864, 911
TatI WGTACW 1 cut(s) 255
TauI GCSGC 2 cut(s) 416, 969
TfiI GAWTC 7 cut(s) 204, 327, 614, 971, 1091, 1172, 1214
Tru1I TTAA 1 cut(s) 690
Tru9I TTAA 1 cut(s) 690
TscAI CASTG 4 cut(s) 139, 214, 670, 823
TseFI GTSAC 1 cut(s) 1106
TseI GCWGC 2 cut(s) 394, 1075
Tsp45I GTSAC 1 cut(s) 1106
TspDTI ATGAA 8 cut(s) 115, 387, 543, 815, 846, 891, 1155, 1185
TspGWI ACGGA 2 cut(s) 1055, 1199
TspRI CASTG 4 cut(s) 139, 214, 670, 823
Van91I CCANNNNNTGG 1 cut(s) 66
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 2 cut(s) 300, 336
XbaI TCTAGA 1 cut(s) 7
XmiI GTMKAC 3 cut(s) 39, 56, 955
XspI CTAG 2 cut(s) 8, 1319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.