pycom05g01860

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010913
Physical Location & Seq
Reverse (-)
2212812 .. 2214227
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g01860.1

Sequence Viewer

Length: 1323 bp
ATGGATCTAGAATTAGGACTCAAGATCACCCATACCGTAGACGATCATGTCTCCTCTGCCAACTTTCGGATTGCCAAGGATAGATCCGGTCCTCTTTTCGTGTCTAAGGAAACCGAAAGCATGTTCATCCTCACCGGCCATCTTAAAGGTTATAGAAAAGAGCGTGTGTGTATTGACATAAATGAAGATGGGACGCAGATAGGCATCAGTGGGGAGAAGCCAGTTCAAGAGAAGGTGATGTCAGGATGGATAATGCACAAGAAAGAGGTGGAGATGAGGGGTTTCAGAAAAGTCTTTCGAATTCCGGATGGAGTGGTTTTGGATCGAATCAAGGCCAAGTTTAATGTACACGAATCAAGTCTCACGATCGTCATGCCAAAATCAGTGAAAGGAAGTCGCGGGGTTGGGATCGAGGAAGTGAAGGAAGATGAAGTGGATACAGGGAAACGGCAGCTCACACAGCCTCCCACAGCTGAAGAAGTCCCTGAAAAAGATGGTTTTAGGGGGAAAATTGAGGGAGAAATTGGTGAGACCGAGAAAACGAAAGTAGAAGAAAATCGTCGAACTGATGCTGTCATCGGAAAGGACACGAGAGGAAGCACAGTTAAGGAAGAAGCTAAAGATTCAAAGGCTCAAAGTACGCAACACAAAACAAAATCTGTTTCCGAAAACAATGAAGAGTCAAAAGTTGCAAAGGTAGAAGAAGCTGATGTGCTTTTGAAAGAGAAAGTTGGAAGAAAGGAATTGGAAACAGATCAAACTGTAGCGAATAGGGTTCGGAAGAGAGAAAATGCTGAAGTAACACGTGAAGTTTACAAGCCTTCACAAGAAAACCAGGTCACTGCTAAGCATGACACAACAGAAGAAAACTCTTCTGAGCTCCCAAAACTGGAAGCGCCAAAGGCAGGGATTGAGGAGCACGTTCGAAACGGACAAGAAAGCGGCGAATCATCAAAGCAAGCAGAAGATACAACAGCTACACAACAAGAAGCGAAAGTTCAACGCGAAAAGAATGCGGATGGAATTCGTAGAGAAGGCGATGCTCAGCTACCAAAGCAAGAATCCATCAAAGAAGGTCAGGGAAGCGGAGTTCAAGAAAGAGCGAAGAACGAAGAACACATCAAAGAGAAGGAAGAGGATGAATCTTCCGAAATGGAGGAACAAAGTGATGTGGATGATAAGAAATCAAGTGATGATCAAGAGAACAAGGATGGAAAGGAAAAGTCTAATCTGCTGTGCTCTCCTTTTATAATTGCAGGTTCAGCACTTATTGTGTCCCTGGTGATGCTAGCAATCAACAAGATTAGAACTAGAAAAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

441

Amino Acids

49.29

Weight (kDa)

5.55

Isoelectric Point (pI)

43.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1250
AasI GACNNNNNNGTC 1 cut(s) 47
Acc36I ACCTGC 1 cut(s) 1247
AccI GTMKAC 1 cut(s) 39
AccII CGCG 2 cut(s) 399, 1005
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 4 cut(s) 399, 942, 1016, 1086
AclWI GGATC 4 cut(s) 12, 78, 330, 416
AcoI YGGCCR 1 cut(s) 136
AcsI RAATTY 2 cut(s) 300, 1023
AcuI CTGAAG 2 cut(s) 495, 816
AcvI CACGTG 1 cut(s) 806
AfaI GTAC 2 cut(s) 348, 640
AfiI CCNNNNNNNGG 3 cut(s) 66, 889, 905
AflIII ACRYGT 1 cut(s) 803
AgsI TTSAA 5 cut(s) 227, 627, 721, 1001, 1094
AjnI CCWGG 2 cut(s) 834, 1278
AloI GAACNNNNNNTCC 2 cut(s) 1074, 1106
AluBI AGCT 7 cut(s) 454, 473, 617, 707, 880, 977, 1048
AluI AGCT 7 cut(s) 454, 473, 617, 707, 880, 977, 1048
Alw21I GWGCWC 3 cut(s) 882, 921, 1241
Alw26I GTCTC 3 cut(s) 55, 365, 524
AlwI GGATC 4 cut(s) 12, 78, 330, 416
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 2 cut(s) 136, 333
ApeKI GCWGC 1 cut(s) 451
ApoI RAATTY 2 cut(s) 300, 1023
AspLEI GCGC 1 cut(s) 898
AspS9I GGNCC 1 cut(s) 89
AsuHPI GGTGA 5 cut(s) 19, 124, 247, 539, 1294
AsuII TTCGAA 2 cut(s) 298, 925
AsuNHI GCTAGC 1 cut(s) 1288
AvaII GGWCC 1 cut(s) 89
BanII GRGCYC 1 cut(s) 882
BauI CACGAG 1 cut(s) 589
BbrPI CACGTG 1 cut(s) 806
Bbv12I GWGCWC 3 cut(s) 882, 921, 1241
BbvI GCAGC 1 cut(s) 463
BccI CCATC 8 cut(s) 147, 182, 240, 302, 488, 1013, 1073, 1205
BceAI ACGGC 1 cut(s) 464
BcgI CGANNNNNNTGC 4 cut(s) 355, 389, 995, 1029
BciT130I CCWGG 2 cut(s) 836, 1280
BciVI GTATCC 1 cut(s) 430
BclI TGATCA 1 cut(s) 1195
BcoDI GTCTC 3 cut(s) 55, 365, 524
BfaI CTAG 3 cut(s) 8, 1289, 1311
BfmI CTRYAG 1 cut(s) 762
BfoI RGCGCY 1 cut(s) 899
BfuAI ACCTGC 1 cut(s) 1247
BfuI GTATCC 1 cut(s) 430
BisI GCNGC 2 cut(s) 452, 943
BlpI GCTNAGC 2 cut(s) 846, 1044
BlsI GCNGC 2 cut(s) 453, 944
Bme1390I CCNGG 2 cut(s) 836, 1280
Bme18I GGWCC 1 cut(s) 89
BmgT120I GGNCC 1 cut(s) 89
BmrFI CCNGG 2 cut(s) 836, 1280
BmsI GCATC 4 cut(s) 213, 559, 1030, 1275
BmtI GCTAGC 1 cut(s) 1292
Bpu1102I GCTNAGC 2 cut(s) 846, 1044
Bpu14I TTCGAA 2 cut(s) 298, 925
BpuEI CTTGAG 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 806
BsaBI GATNNNNATC 1 cut(s) 203
BsaI GGTCTC 1 cut(s) 524
BsaJI CCNNGG 2 cut(s) 75, 1278
BsaWI WCCGGW 2 cut(s) 86, 304
BsaXI ACNNNNNCTCC 2 cut(s) 448, 478
Bsc4I CCNNNNNNNGG 3 cut(s) 66, 889, 905
Bse118I RCCGGY 1 cut(s) 134
Bse1I ACTGG 2 cut(s) 221, 894
Bse8I GATNNNNATC 1 cut(s) 203
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 2 cut(s) 836, 1280
BseDI CCNNGG 2 cut(s) 75, 1278
BseGI GGATG 7 cut(s) 126, 251, 313, 1024, 1144, 1180, 1216
BseJI GATNNNNATC 1 cut(s) 203
BseLI CCNNNNNNNGG 3 cut(s) 66, 889, 905
BseMII CTCAG 2 cut(s) 867, 1058
BseNI ACTGG 2 cut(s) 221, 894
BseRI GAGGAG 2 cut(s) 43, 929
BseXI GCAGC 1 cut(s) 463
Bsh1236I CGCG 2 cut(s) 399, 1005
Bsh1285I CGRYCG 1 cut(s) 369
BshFI GGCC 2 cut(s) 138, 335
BsiEI CGRYCG 1 cut(s) 369
BsiHKAI GWGCWC 3 cut(s) 882, 921, 1241
BsiSI CCGG 3 cut(s) 87, 135, 305
BslFI GGGAC 3 cut(s) 205, 467, 1261
BslI CCNNNNNNNGG 3 cut(s) 66, 889, 905
BsmAI GTCTC 3 cut(s) 55, 365, 524
BsmFI GGGAC 3 cut(s) 205, 467, 1261
BsmI GAATGC 1 cut(s) 1018
BsnI GGCC 2 cut(s) 138, 335
Bso31I GGTCTC 1 cut(s) 524
Bsp119I TTCGAA 2 cut(s) 298, 925
Bsp1286I GDGCHC 3 cut(s) 882, 921, 1241
Bsp13I TCCGGA 1 cut(s) 304
Bsp1407I TGTACA 1 cut(s) 346
Bsp143I GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
Bsp1720I GCTNAGC 2 cut(s) 846, 1044
BspACI CCGC 4 cut(s) 399, 942, 1016, 1086
BspANI GGCC 2 cut(s) 138, 335
BspCNI CTCAG 2 cut(s) 868, 1057
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 2 cut(s) 399, 1005
BspMI ACCTGC 1 cut(s) 1247
BspOI GCTAGC 1 cut(s) 1292
BspPI GGATC 4 cut(s) 12, 78, 330, 416
BspT104I TTCGAA 2 cut(s) 298, 925
BspTNI GGTCTC 1 cut(s) 524
BsrFI RCCGGY 1 cut(s) 134
BsrGI TGTACA 1 cut(s) 346
BsrI ACTGG 2 cut(s) 221, 894
BssAI RCCGGY 1 cut(s) 134
BssECI CCNNGG 2 cut(s) 75, 1278
BssMI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
BssSI CACGAG 1 cut(s) 589
BssT1I CCWWGG 1 cut(s) 75
Bst2BI CACGAG 1 cut(s) 589
Bst2UI CCWGG 2 cut(s) 836, 1280
Bst4CI ACNGT 3 cut(s) 37, 604, 763
Bst6I CTCTTC 4 cut(s) 672, 776, 877, 1128
BstAUI TGTACA 1 cut(s) 346
BstBAI YACGTR 1 cut(s) 806
BstBI TTCGAA 2 cut(s) 298, 925
BstC8I GCNNGC 2 cut(s) 960, 1290
BstDEI CTNAG 4 cut(s) 105, 846, 876, 1044
BstF5I GGATG 7 cut(s) 126, 251, 313, 1024, 1144, 1180, 1216
BstFNI CGCG 2 cut(s) 399, 1005
BstH2I RGCGCY 1 cut(s) 899
BstHHI GCGC 1 cut(s) 898
BstKTI GATC 9 cut(s) 7, 27, 46, 86, 325, 369, 411, 757, 1198
BstMAI GTCTC 3 cut(s) 55, 365, 524
BstMBI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
BstMCI CGRYCG 1 cut(s) 369
BstMWI GCNNNNNNNGC 4 cut(s) 460, 902, 1054, 1262
BstNI CCWGG 2 cut(s) 836, 1280
BstNSI RCATGY 1 cut(s) 124
BstSCI CCNGG 2 cut(s) 834, 1278
BstSFI CTRYAG 1 cut(s) 762
BstUI CGCG 2 cut(s) 399, 1005
BstV1I GCAGC 1 cut(s) 463
BstX2I RGATCY 2 cut(s) 4, 83
BstYI RGATCY 2 cut(s) 4, 83
BsuI GTATCC 1 cut(s) 430
BsuRI GGCC 2 cut(s) 138, 335
BtgZI GCGATG 1 cut(s) 1053
BtsCI GGATG 7 cut(s) 126, 251, 313, 1024, 1144, 1180, 1216
BtsI GCAGTG 1 cut(s) 840
BtsIMutI CAGTG 3 cut(s) 214, 390, 840
BveI ACCTGC 1 cut(s) 1247
Cac8I GCNNGC 2 cut(s) 960, 1290
CfoI GCGC 1 cut(s) 898
Cfr10I RCCGGY 1 cut(s) 134
Cfr13I GGNCC 1 cut(s) 89
CseI GACGC 1 cut(s) 202
CsiI ACCWGGT 1 cut(s) 834
Csp6I GTAC 2 cut(s) 347, 639
CviAII CATG 4 cut(s) 47, 121, 373, 851
CviQI GTAC 2 cut(s) 347, 639
DdeI CTNAG 4 cut(s) 105, 846, 876, 1044
DpnI GATC 9 cut(s) 6, 26, 45, 85, 324, 368, 410, 756, 1197
DpnII GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
DrdI GACNNNNNNGTC 1 cut(s) 47
DseDI GACNNNNNNGTC 1 cut(s) 47
EaeI YGGCCR 1 cut(s) 136
Eam1104I CTCTTC 4 cut(s) 672, 776, 877, 1128
EarI CTCTTC 4 cut(s) 672, 776, 877, 1128
Ecl136II GAGCTC 1 cut(s) 880
Eco130I CCWWGG 1 cut(s) 75
Eco24I GRGCYC 1 cut(s) 882
Eco31I GGTCTC 1 cut(s) 524
Eco47I GGWCC 1 cut(s) 89
Eco53kI GAGCTC 1 cut(s) 880
Eco57I CTGAAG 2 cut(s) 495, 816
Eco72I CACGTG 1 cut(s) 806
EcoICRI GAGCTC 1 cut(s) 880
EcoRI GAATTC 2 cut(s) 300, 1023
EcoRII CCWGG 2 cut(s) 834, 1278
EcoT14I CCWWGG 1 cut(s) 75
EcoT38I GRGCYC 1 cut(s) 882
ErhI CCWWGG 1 cut(s) 75
FaeI CATG 4 cut(s) 50, 124, 376, 854
FaiI YATR 8 cut(s) 33, 48, 122, 153, 179, 374, 852, 1250
FaqI GGGAC 3 cut(s) 205, 467, 1261
FatI CATG 4 cut(s) 46, 120, 372, 850
FauI CCCGC 1 cut(s) 392
FbaI TGATCA 1 cut(s) 1195
FblI GTMKAC 1 cut(s) 39
Fnu4HI GCNGC 2 cut(s) 452, 943
FokI GGATG 7 cut(s) 113, 258, 320, 1031, 1151, 1187, 1223
FriOI GRGCYC 1 cut(s) 882
Fsp4HI GCNGC 2 cut(s) 452, 943
FspBI CTAG 3 cut(s) 8, 1289, 1311
GlaI GCGC 1 cut(s) 897
GluI GCNGC 2 cut(s) 452, 943
HaeII RGCGCY 1 cut(s) 899
HaeIII GGCC 2 cut(s) 138, 335
HapII CCGG 3 cut(s) 87, 135, 305
HgaI GACGC 1 cut(s) 202
HhaI GCGC 1 cut(s) 898
Hin1II CATG 4 cut(s) 50, 124, 376, 854
Hin6I GCGC 1 cut(s) 896
HinP1I GCGC 1 cut(s) 896
HinfI GANTC 8 cut(s) 18, 327, 353, 623, 680, 947, 1061, 1142
HpaII CCGG 3 cut(s) 87, 135, 305
HphI GGTGA 5 cut(s) 19, 124, 247, 539, 1294
Hpy166II GTNNAC 3 cut(s) 40, 349, 814
Hpy188I TCNGA 7 cut(s) 69, 287, 581, 667, 780, 877, 1150
Hpy188III TCNNGA 8 cut(s) 8, 22, 227, 243, 305, 364, 1094, 1199
Hpy8I GTNNAC 3 cut(s) 40, 349, 814
Hpy99I CGWCG 1 cut(s) 564
HpyAV CCTTC 6 cut(s) 226, 415, 831, 1028, 1067, 1123
HpyCH4III ACNGT 3 cut(s) 37, 604, 763
HpyCH4IV ACGT 2 cut(s) 805, 921
HpyCH4V TGCA 3 cut(s) 256, 692, 1256
HpyF10VI GCNNNNNNNGC 4 cut(s) 460, 902, 1054, 1262
HpyF3I CTNAG 4 cut(s) 105, 846, 876, 1044
HpySE526I ACGT 2 cut(s) 805, 921
Hsp92II CATG 4 cut(s) 50, 124, 376, 854
HspAI GCGC 1 cut(s) 896
Kpn2I TCCGGA 1 cut(s) 304
Ksp22I TGATCA 1 cut(s) 1195
Kzo9I GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
LmnI GCTCC 2 cut(s) 885, 916
Lsp1109I GCAGC 1 cut(s) 463
LweI GCATC 4 cut(s) 213, 559, 1030, 1275
MabI ACCWGGT 1 cut(s) 834
MaeI CTAG 3 cut(s) 8, 1289, 1311
MaeII ACGT 2 cut(s) 805, 921
MaeIII GTNAC 2 cut(s) 799, 838
MalI GATC 9 cut(s) 6, 26, 45, 85, 324, 368, 410, 756, 1197
MboI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
MflI RGATCY 2 cut(s) 4, 83
MhlI GDGCHC 3 cut(s) 882, 921, 1241
MluCI AATT 7 cut(s) 11, 300, 510, 522, 743, 1023, 1251
MlyI GAGTC 2 cut(s) 12, 689
MmeI TCCRAC 1 cut(s) 712
MroI TCCGGA 1 cut(s) 304
MseI TTAA 3 cut(s) 144, 342, 606
MspA1I CMGCKG 1 cut(s) 473
MspI CCGG 3 cut(s) 87, 135, 305
MspR9I CCNGG 2 cut(s) 836, 1280
Mva1269I GAATGC 1 cut(s) 1018
MvaI CCWGG 2 cut(s) 836, 1280
MvnI CGCG 2 cut(s) 399, 1005
MwoI GCNNNNNNNGC 4 cut(s) 460, 902, 1054, 1262
NdeII GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
NheI GCTAGC 1 cut(s) 1288
NlaIII CATG 4 cut(s) 50, 124, 376, 854
NmuCI GTSAC 1 cut(s) 838
NspI RCATGY 1 cut(s) 124
NspV TTCGAA 2 cut(s) 298, 925
PctI GAATGC 1 cut(s) 1018
PfeI GAWTC 6 cut(s) 327, 353, 623, 947, 1061, 1142
PkrI GCNGC 2 cut(s) 453, 944
Ple19I CGATCG 1 cut(s) 369
PleI GAGTC 2 cut(s) 12, 688
PmaCI CACGTG 1 cut(s) 806
PmlI CACGTG 1 cut(s) 806
PpsI GAGTC 2 cut(s) 12, 688
Ppu21I YACGTR 1 cut(s) 806
PsiI TTATAA 1 cut(s) 1250
Psp124BI GAGCTC 1 cut(s) 882
Psp6I CCWGG 2 cut(s) 834, 1278
PspCI CACGTG 1 cut(s) 806
PspGI CCWGG 2 cut(s) 834, 1278
PspPI GGNCC 1 cut(s) 89
PsuI RGATCY 2 cut(s) 4, 83
PvuI CGATCG 1 cut(s) 369
PvuII CAGCTG 1 cut(s) 473
RsaI GTAC 2 cut(s) 348, 640
RsaNI GTAC 2 cut(s) 347, 639
SacI GAGCTC 1 cut(s) 882
SaqAI TTAA 3 cut(s) 144, 342, 606
SatI GCNGC 2 cut(s) 452, 943
Sau3AI GATC 9 cut(s) 4, 24, 43, 83, 322, 366, 408, 754, 1195
Sau96I GGNCC 1 cut(s) 89
SchI GAGTC 2 cut(s) 12, 689
ScrFI CCNGG 2 cut(s) 836, 1280
SduI GDGCHC 3 cut(s) 882, 921, 1241
SexAI ACCWGGT 1 cut(s) 834
SfaNI GCATC 4 cut(s) 213, 559, 1030, 1275
SfcI CTRYAG 1 cut(s) 762
SfuI TTCGAA 2 cut(s) 298, 925
SinI GGWCC 1 cut(s) 89
SmlI CTYRAG 1 cut(s) 20
SmoI CTYRAG 1 cut(s) 20
Sse9I AATT 7 cut(s) 11, 300, 510, 522, 743, 1023, 1251
SsiI CCGC 4 cut(s) 399, 942, 1016, 1086
SspMI CTAG 3 cut(s) 8, 1289, 1311
SstI GAGCTC 1 cut(s) 882
StyD4I CCNGG 2 cut(s) 834, 1278
StyI CCWWGG 1 cut(s) 75
TaaI ACNGT 3 cut(s) 37, 604, 763
TaiI ACGT 2 cut(s) 808, 924
TaqI TCGA 5 cut(s) 298, 325, 411, 562, 925
TaqII GACCGA 1 cut(s) 548
TasI AATT 7 cut(s) 11, 300, 510, 522, 743, 1023, 1251
TatI WGTACW 1 cut(s) 346
TauI GCSGC 1 cut(s) 945
TfiI GAWTC 6 cut(s) 327, 353, 623, 947, 1061, 1142
Tru1I TTAA 3 cut(s) 144, 342, 606
Tru9I TTAA 3 cut(s) 144, 342, 606
TscAI CASTG 3 cut(s) 214, 390, 847
TseFI GTSAC 1 cut(s) 838
TseI GCWGC 1 cut(s) 451
Tsp45I GTSAC 1 cut(s) 838
TspDTI ATGAA 5 cut(s) 115, 198, 444, 690, 1155
TspGWI ACGGA 1 cut(s) 945
TspRI CASTG 3 cut(s) 214, 390, 847
VpaK11BI GGWCC 1 cut(s) 89
XapI RAATTY 2 cut(s) 300, 1023
XbaI TCTAGA 1 cut(s) 7
XceI RCATGY 1 cut(s) 124
XmiI GTMKAC 1 cut(s) 39
XspI CTAG 3 cut(s) 8, 1289, 1311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.