Rmu_sc0010674.1_g000001

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0010674.1
OG0010913
Physical Location & Seq
Forward (+)
167 .. 3244
3078 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0010674.1_g000001.1.cds

Sequence Viewer

Length: 1440 bp
atggctccagtggcagcccctgaacccagttccaagacaggttctggtggcagtggtggtggtgtagtgtcatcttctccaacatatccatggcctttccaacctcaccaagcttccttatcgccaagtccagggccaagtgtcggctctttaattcctaataaaggtgctggcatgccatttatcaacagcaaccctgctgttcctttgcctactggtgaagtcgattctgctacaattcgccctataccaacatctggccaccagggacaggtgttggtggggttatttgctgcgcaaatggctctatttagtgtgaaagtaaaatctggagtttctaaggtgattcctagtcccctaaatccagaaatggatcttgaattaggactcaagatcaccaaaaatgcagatgatcatactacctcttctgattttcggattgcgaaggagaaatctagtcctgttttcctatccagcgaaacagaaagcgcgtacgtcctcactgcccatcttaaaggttatagaaaagagcatgtcaatattgacataaatgaagacgagactcagctaggcattgctgtggagaagccagttcaagagaaggtgatgtcaggatggtcaatgaacaagaaagaaggggagacaaggggattcagaaaagtctttcggatcccagatggagtggttttgagtcgaatcaaggccaagattaatgaacaagactcgactctcagaatcttcatgccaaaagtggtgaaaggatatgttggtgttggggttggggttgaggaactgaaagaagaagaggtgatcatagggaaaccacagatgacagaagctgcaagtgatgaggttcctgaaaaaatcagtgagaaaatgaaaatggataagaaccctcaggctaaaggaaaagaggtagataaagaagagtacgcgaaagaagttgaattagaggatcaagatttgcagaccaatgatcgaagagaaactgaaggtggagggatggagtcagaagaaagtgaagaacgaaagattgcaaagacaaaaaaagctgatagaattgagaaacagagagaggatgctaaaaaaagaatcaaggaaaaaacacatggaggagtcaagaatgataaaagcactgaagcaactcatcaagttgctaaaccatcacaagaaattgaagaaattgaaccaggatcgagaccccccaacgcgaacgaaatgccaaaagcaacgccagagaagaaggaagtacttgacaagggtgaatcttctcaaatgcggaaaggaaaagatgtgtgtgagaaatcaactccagagaagaagcctgctgcgtcagaaaagtccaagttgctgtccactcctttgatcattgcaggttcagcccttctcgtcactattgtactgatagcaattcacaggattaggaaaagaaagcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HSP20

Protein Analysis

479

Amino Acids

52.16

Weight (kDa)

8.22

Isoelectric Point (pI)

50.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010913)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G76770
fragaria_vesca FvH4_2g13660 FvH4_2g13660
malus_domestica MD05G1026800.v1.1 MD10G1026100.v1.1
prunus_persica Prupe.8G031300_v2.0.a1
pyrus_communis pycom05g01860 pycom10g01750
rosa_chinensis RchiOBHm_Chr6g0275301
rosa_laevigata RLG00000013474
rosa_multiflora Rmu_sc0010674.1_g000001 Rmu_sc0011469.1_g000003 Rmu_sc0015244.1_g000002
rosa_roxburghii Rroxscaffold_7G00193420
rosa_rugosa Rorug06G0091000
rosa_samantha Rh6AG204600 Rh6BG207500 Rh6CG210000 Rh6DG200400
rosa_wichuraiana Rw6G017760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 297
Acc36I ACCTGC 1 cut(s) 1364
AccB7I CCANNNNNTGG 1 cut(s) 257
AccII CGCG 3 cut(s) 491, 935, 1211
AciI CCGC 1 cut(s) 1279
AclWI GGATC 5 cut(s) 381, 664, 677, 963, 1201
AcoI YGGCCR 1 cut(s) 259
AcuI CTGAAG 2 cut(s) 1011, 1158
AfaI GTAC 4 cut(s) 494, 932, 1251, 1401
AfiI CCNNNNNNNGG 5 cut(s) 131, 143, 164, 257, 271
AgsI TTSAA 5 cut(s) 380, 596, 947, 1178, 1187
AjnI CCWGG 3 cut(s) 130, 264, 1189
AluBI AGCT 4 cut(s) 113, 568, 839, 1052
AluI AGCT 4 cut(s) 113, 568, 839, 1052
Alw26I GTCTC 3 cut(s) 554, 635, 1192
AlwI GGATC 5 cut(s) 381, 664, 677, 963, 1201
AlwNI CAGNNNCTG 3 cut(s) 20, 44, 839
AoxI GGCC 4 cut(s) 92, 134, 259, 702
ApeKI GCWGC 4 cut(s) 14, 293, 839, 1328
AseI ATTAAT 1 cut(s) 711
AspLEI GCGC 2 cut(s) 298, 491
AspS9I GGNCC 1 cut(s) 134
AsuHPI GGTGA 8 cut(s) 98, 230, 355, 388, 616, 766, 820, 1274
AxyI CCTNAGG 1 cut(s) 897
BalI TGGCCA 1 cut(s) 261
BamHI GGATCC 1 cut(s) 669
BbsI GAAGAC 1 cut(s) 561
BbvI GCAGC 4 cut(s) 26, 280, 826, 1315
BccI CCATC 5 cut(s) 516, 609, 671, 997, 1171
BcgI CGANNNNNNTGC 2 cut(s) 1201, 1235
BciT130I CCWGG 3 cut(s) 132, 266, 1191
BclI TGATCA 3 cut(s) 412, 810, 1365
BcoDI GTCTC 3 cut(s) 554, 635, 1192
BfaI CTAG 3 cut(s) 351, 456, 569
BfuAI ACCTGC 1 cut(s) 1364
BisI GCNGC 4 cut(s) 15, 294, 840, 1329
BlsI GCNGC 4 cut(s) 16, 295, 841, 1330
BmcAI AGTACT 1 cut(s) 1251
Bme1390I CCNGG 3 cut(s) 132, 266, 1191
BmgT120I GGNCC 1 cut(s) 134
BmiI GGNNCC 3 cut(s) 6, 671, 855
BmrFI CCNGG 3 cut(s) 132, 266, 1191
BmrI ACTGGG 1 cut(s) 21
BmsI GCATC 1 cut(s) 1069
BmuI ACTGGG 1 cut(s) 21
BpiI GAAGAC 1 cut(s) 561
BpmI CTGGAG 2 cut(s) 351, 1296
BpuEI CTTGAG 1 cut(s) 374
BsaI GGTCTC 1 cut(s) 1192
BsaJI CCNNGG 3 cut(s) 89, 131, 265
Bsc4I CCNNNNNNNGG 5 cut(s) 131, 143, 164, 257, 271
Bse1I ACTGG 4 cut(s) 8, 27, 220, 590
Bse21I CCTNAGG 1 cut(s) 897
Bse3DI GCAATG 2 cut(s) 573, 1368
BseBI CCWGG 3 cut(s) 132, 266, 1191
BseDI CCNNGG 3 cut(s) 89, 131, 265
BseGI GGATG 3 cut(s) 620, 1008, 1084
BseLI CCNNNNNNNGG 5 cut(s) 131, 143, 164, 257, 271
BseMI GCAATG 2 cut(s) 573, 1368
BseMII CTCAG 3 cut(s) 578, 745, 911
BseNI ACTGG 4 cut(s) 8, 27, 220, 590
BseRI GAGGAG 1 cut(s) 1128
BseXI GCAGC 4 cut(s) 26, 280, 826, 1315
Bsh1236I CGCG 3 cut(s) 491, 935, 1211
BshFI GGCC 4 cut(s) 94, 136, 261, 704
BsiWI CGTACG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 282, 339
BslI CCNNNNNNNGG 5 cut(s) 131, 143, 164, 257, 271
BsmAI GTCTC 3 cut(s) 554, 635, 1192
BsmFI GGGAC 2 cut(s) 282, 339
BsnI GGCC 4 cut(s) 94, 136, 261, 704
Bso31I GGTCTC 1 cut(s) 1192
Bsp143I GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
Bsp19I CCATGG 1 cut(s) 89
BspACI CCGC 1 cut(s) 1279
BspANI GGCC 4 cut(s) 94, 136, 261, 704
BspCNI CTCAG 3 cut(s) 577, 744, 910
BspFNI CGCG 3 cut(s) 491, 935, 1211
BspLI GGNNCC 3 cut(s) 6, 671, 855
BspMI ACCTGC 1 cut(s) 1364
BspPI GGATC 5 cut(s) 381, 664, 677, 963, 1201
BspTNI GGTCTC 1 cut(s) 1192
BsrDI GCAATG 2 cut(s) 573, 1368
BsrI ACTGG 4 cut(s) 8, 27, 220, 590
BssECI CCNNGG 3 cut(s) 89, 131, 265
BssMI GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
BssT1I CCWWGG 1 cut(s) 89
Bst2UI CCWGG 3 cut(s) 132, 266, 1191
Bst6I CTCTTC 4 cut(s) 430, 798, 921, 976
BstC8I GCNNGC 3 cut(s) 172, 176, 1326
BstDEI CTNAG 4 cut(s) 339, 564, 731, 897
BstDSI CCRYGG 1 cut(s) 89
BstENI CCTNNNNNAGG 1 cut(s) 162
BstF5I GGATG 3 cut(s) 620, 1008, 1084
BstFNI CGCG 3 cut(s) 491, 935, 1211
BstHHI GCGC 2 cut(s) 298, 491
BstKTI GATC 9 cut(s) 376, 396, 415, 672, 813, 958, 979, 1196, 1368
BstMAI GTCTC 3 cut(s) 554, 635, 1192
BstMBI GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
BstMWI GCNNNNNNNGC 3 cut(s) 11, 302, 1379
BstNI CCWGG 3 cut(s) 132, 266, 1191
BstNSI RCATGY 2 cut(s) 178, 536
BstSCI CCNGG 3 cut(s) 130, 264, 1189
BstUI CGCG 3 cut(s) 491, 935, 1211
BstV1I GCAGC 4 cut(s) 26, 280, 826, 1315
BstV2I GAAGAC 1 cut(s) 561
BstX2I RGATCY 2 cut(s) 373, 669
BstYI RGATCY 2 cut(s) 373, 669
Bsu36I CCTNAGG 1 cut(s) 897
BsuRI GGCC 4 cut(s) 94, 136, 261, 704
BtgI CCRYGG 1 cut(s) 89
BtsCI GGATG 3 cut(s) 620, 1008, 1084
BtsI GCAGTG 2 cut(s) 58, 501
BtsIMutI CAGTG 5 cut(s) 15, 58, 501, 874, 1134
BveI ACCTGC 1 cut(s) 1364
Cac8I GCNNGC 3 cut(s) 172, 176, 1326
CaiI CAGNNNCTG 3 cut(s) 20, 44, 839
CfoI GCGC 2 cut(s) 298, 491
Cfr13I GGNCC 1 cut(s) 134
CseI GACGC 1 cut(s) 1320
Csp6I GTAC 4 cut(s) 493, 931, 1250, 1400
CviAII CATG 5 cut(s) 90, 175, 533, 742, 1109
CviQI GTAC 4 cut(s) 493, 931, 1250, 1400
DdeI CTNAG 4 cut(s) 339, 564, 731, 897
DpnI GATC 9 cut(s) 375, 395, 414, 671, 812, 957, 978, 1195, 1367
DpnII GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
EaeI YGGCCR 1 cut(s) 259
Eam1104I CTCTTC 4 cut(s) 430, 798, 921, 976
EarI CTCTTC 4 cut(s) 430, 798, 921, 976
Eco130I CCWWGG 1 cut(s) 89
Eco31I GGTCTC 1 cut(s) 1192
Eco57I CTGAAG 2 cut(s) 1011, 1158
Eco81I CCTNAGG 1 cut(s) 897
EcoNI CCTNNNNNAGG 1 cut(s) 162
EcoRII CCWGG 3 cut(s) 130, 264, 1189
EcoT14I CCWWGG 1 cut(s) 89
ErhI CCWWGG 1 cut(s) 89
FaeI CATG 5 cut(s) 93, 178, 536, 745, 1112
FaqI GGGAC 2 cut(s) 282, 339
FatI CATG 5 cut(s) 89, 174, 532, 741, 1108
FbaI TGATCA 3 cut(s) 412, 810, 1365
Fnu4HI GCNGC 4 cut(s) 15, 294, 840, 1329
FokI GGATG 3 cut(s) 627, 1015, 1091
Fsp4HI GCNGC 4 cut(s) 15, 294, 840, 1329
FspBI CTAG 3 cut(s) 351, 456, 569
FspI TGCGCA 1 cut(s) 297
GlaI GCGC 2 cut(s) 297, 490
GluI GCNGC 4 cut(s) 15, 294, 840, 1329
GsuI CTGGAG 2 cut(s) 351, 1296
HaeIII GGCC 4 cut(s) 94, 136, 261, 704
HgaI GACGC 1 cut(s) 1320
HhaI GCGC 2 cut(s) 298, 491
Hin1II CATG 5 cut(s) 93, 178, 536, 745, 1112
Hin6I GCGC 2 cut(s) 296, 489
HinP1I GCGC 2 cut(s) 296, 489
HindIII AAGCTT 1 cut(s) 111
HphI GGTGA 8 cut(s) 98, 230, 355, 388, 616, 766, 820, 1274
Hpy166II GTNNAC 1 cut(s) 1356
Hpy188I TCNGA 7 cut(s) 430, 438, 656, 669, 734, 1012, 1336
Hpy8I GTNNAC 1 cut(s) 1356
HpyAV CCTTC 6 cut(s) 439, 595, 629, 986, 1237, 1394
HpyCH4IV ACGT 1 cut(s) 495
HpyCH4V TGCA 5 cut(s) 407, 842, 967, 1037, 1373
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 302, 1379
HpyF3I CTNAG 4 cut(s) 339, 564, 731, 897
HpySE526I ACGT 1 cut(s) 495
Hsp92II CATG 5 cut(s) 93, 178, 536, 745, 1112
HspAI GCGC 2 cut(s) 296, 489
Ksp22I TGATCA 3 cut(s) 412, 810, 1365
Kzo9I GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 4 cut(s) 26, 280, 826, 1315
LweI GCATC 1 cut(s) 1069
MaeI CTAG 3 cut(s) 351, 456, 569
MaeII ACGT 1 cut(s) 495
MaeIII GTNAC 1 cut(s) 1390
MalI GATC 9 cut(s) 375, 395, 414, 671, 812, 957, 978, 1195, 1367
MboI GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
MflI RGATCY 2 cut(s) 373, 669
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 8 cut(s) 153, 237, 380, 947, 1059, 1173, 1182, 1410
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 7 cut(s) 381, 556, 700, 716, 721, 1016, 1125
MmeI TCCRAC 2 cut(s) 104, 124
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 3 cut(s) 152, 513, 711
MslI CAYNNNNRTG 2 cut(s) 88, 578
Msp20I TGGCCA 1 cut(s) 261
MspR9I CCNGG 3 cut(s) 132, 266, 1191
MvaI CCWGG 3 cut(s) 132, 266, 1191
MvnI CGCG 3 cut(s) 491, 935, 1211
MwoI GCNNNNNNNGC 3 cut(s) 11, 302, 1379
NcoI CCATGG 1 cut(s) 89
NdeII GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
NlaIII CATG 5 cut(s) 93, 178, 536, 745, 1112
NlaIV GGNNCC 3 cut(s) 6, 671, 855
NmuCI GTSAC 1 cut(s) 1390
NsbI TGCGCA 1 cut(s) 297
NspI RCATGY 2 cut(s) 178, 536
PaeI GCATGC 1 cut(s) 178
PfeI GAWTC 7 cut(s) 227, 346, 651, 696, 735, 1092, 1265
Pfl23II CGTACG 1 cut(s) 492
PflMI CCANNNNNTGG 1 cut(s) 257
PkrI GCNGC 4 cut(s) 16, 295, 841, 1330
PleI GAGTC 7 cut(s) 381, 556, 699, 716, 721, 1015, 1124
PpsI GAGTC 7 cut(s) 381, 556, 699, 716, 721, 1015, 1124
PshBI ATTAAT 1 cut(s) 711
Psp6I CCWGG 3 cut(s) 130, 264, 1189
PspGI CCWGG 3 cut(s) 130, 264, 1189
PspLI CGTACG 1 cut(s) 492
PspN4I GGNNCC 3 cut(s) 6, 671, 855
PspPI GGNCC 1 cut(s) 134
PstNI CAGNNNCTG 3 cut(s) 20, 44, 839
PsuI RGATCY 2 cut(s) 373, 669
RsaI GTAC 4 cut(s) 494, 932, 1251, 1401
RsaNI GTAC 4 cut(s) 493, 931, 1250, 1400
RseI CAYNNNNRTG 2 cut(s) 88, 578
SaqAI TTAA 3 cut(s) 152, 513, 711
SatI GCNGC 4 cut(s) 15, 294, 840, 1329
Sau3AI GATC 9 cut(s) 373, 393, 412, 669, 810, 955, 976, 1193, 1365
Sau96I GGNCC 1 cut(s) 134
ScaI AGTACT 1 cut(s) 1251
SchI GAGTC 7 cut(s) 381, 556, 700, 716, 721, 1016, 1125
ScrFI CCNGG 3 cut(s) 132, 266, 1191
SfaNI GCATC 1 cut(s) 1069
SmiMI CAYNNNNRTG 2 cut(s) 88, 578
SmlI CTYRAG 1 cut(s) 389
SmoI CTYRAG 1 cut(s) 389
SphI GCATGC 1 cut(s) 178
Sse9I AATT 8 cut(s) 153, 237, 380, 947, 1059, 1173, 1182, 1410
SsiI CCGC 1 cut(s) 1279
SspI AATATT 1 cut(s) 541
SspMI CTAG 3 cut(s) 351, 456, 569
StyD4I CCNGG 3 cut(s) 130, 264, 1189
StyI CCWWGG 1 cut(s) 89
TaiI ACGT 1 cut(s) 498
TaqI TCGA 5 cut(s) 225, 694, 725, 979, 1196
TasI AATT 8 cut(s) 153, 237, 380, 947, 1059, 1173, 1182, 1410
TatI WGTACW 2 cut(s) 1249, 1399
TfiI GAWTC 7 cut(s) 227, 346, 651, 696, 735, 1092, 1265
Tru1I TTAA 3 cut(s) 152, 513, 711
Tru9I TTAA 3 cut(s) 152, 513, 711
TscAI CASTG 5 cut(s) 15, 58, 508, 874, 1141
TseFI GTSAC 1 cut(s) 1390
TseI GCWGC 4 cut(s) 14, 293, 839, 1328
Tsp45I GTSAC 1 cut(s) 1390
TspDTI ATGAA 5 cut(s) 567, 638, 729, 730, 893
TspRI CASTG 5 cut(s) 15, 58, 508, 874, 1141
Van91I CCANNNNNTGG 1 cut(s) 257
VspI ATTAAT 1 cut(s) 711
XagI CCTNNNNNAGG 1 cut(s) 162
XceI RCATGY 2 cut(s) 178, 536
XcmI CCANNNNNNNNNTGG 1 cut(s) 87
XspI CTAG 3 cut(s) 351, 456, 569
ZrmI AGTACT 1 cut(s) 1251
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.