MD05G1077400.v1.1

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0010502
Physical Location & Seq
Forward (+)
16539802 .. 16543223
3422 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1077400.v1.1.491

Sequence Viewer

Length: 1272 bp
ATGTGGAGCGGTGGAGAAGCTAGTGGTAGTGGCGGTGGCGGTGGTGCTGGCGTCGGCCGTGGAATCTACTGGGGACGGAAGGAGGAGGTCTCTGATTCCAAAGGAATCGTTGTTATCTTCGCTTGGGTTTCGATTCAGGAGAGGCACTTGCAGAATCACGTGGATTTGTACTCCTCTCTCGGTTGGAATTCCCTCGTTTGCCATTCCCATTTTCTCCATGCATTCTATCCCGAAAAGGCCATGTCGTCCGCCTTTGTTATTCTCAACGAGCTTGTTGAGGAGCTAAGGGTTAAGCCATGTCCTGTAGTCTTTGTGGCTCTTTCTGCTGGTACAAAAGCTTGTATGTACAAGGTTTTTCGGATTATTGACGGAATTTGTGAAGGTCAGCTTTATCCGGCTGAATATCGATTGGTCAGAAAGTGTATTACTGGACACATCTATGATTCTGGTCCAGTTGATTTTACAAGTGATCTGGGCACTCAATATGCTCTACACCCAACCATTCTAAAGATGCCTGGATCTTCAAAACTGGTTTCTTGGGTAGCTAAAGGCATTGCTTCCGGTTTGGATGCGTTGTATCTTACTAGATTTGAATCCCAGTGCGCTGAGTATTGGCACGCTCTGTTTTCATCTGTTAATTTGGGTGCTCCATTTCTCGTTCTGTGCTCTGATAAAGATGATCTGGCCCCCTATCATATTATCTGCAATTTCACTCAACGTTTACAAGAACTTGGAGGAGATGTCAAGCTTGTGAAATTGAATGGCTCCCCTCACCTAGGTCATTATAAGCATTATCCAATACAGTATAGGTCTTCCGTTACTCATTTTCTTGAAAAGGCTGCCTCGGTATTTTCTCAAAGAATTAAAGAACTTGAAGGTGAAAATACTAACATGGAAGGCGTGCAAGATGAGATATCTGAACTAATATGTGATCTCCAGAAAGCAGCAGTTAACTCTAATCAGAGCCTTAGAAGAGTTGCAGTGGAGCCAAGCGACCACTTCTTCTTGCCAAGTTCAGCTGAGAATCAAAATAGTAGAGACTCTGGGTCTTTACAAGATGAAAAAAAAGAAAGATCCGTCTCTCTGCCCAGTCCCCCAAGCATTAGTGCACATAGTGTACTCGGCCAAGTCCTGTTTGATGTTTGTGTCCCTAAGAACGTTGAGGGTTGGGATATTAAATTTTGCGGGTCTTTGAATGGGCAGCCATTTGCCTCTGCTCGTAGGAATTCACCTCCTCGTGGTCTCAAAAGCTTTCGTCGCTCAAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

424

Amino Acids

46.69

Weight (kDa)

7.22

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 142 - 390 5.6e-28 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 786
AccBSI CCGCTC 1 cut(s) 9
AciI CCGC 5 cut(s) 9, 33, 39, 249, 1185
AclI AACGTT 2 cut(s) 718, 1158
AclWI GGATC 2 cut(s) 526, 1068
AcoI YGGCCR 2 cut(s) 55, 1123
AcsI RAATTY 4 cut(s) 187, 372, 1178, 1225
AcvI CACGTG 1 cut(s) 160
AcyI GRCGYC 1 cut(s) 51
AdeI CACNNNGTG 1 cut(s) 1115
AfaI GTAC 4 cut(s) 170, 331, 347, 1119
AfiI CCNNNNNNNGG 2 cut(s) 776, 1238
AgsI TTSAA 6 cut(s) 525, 593, 760, 833, 875, 1195
AhdI GACNNNNNGTC 1 cut(s) 1045
AjnI CCWGG 1 cut(s) 514
AluBI AGCT 9 cut(s) 20, 271, 283, 338, 388, 545, 748, 1019, 1251
AluI AGCT 9 cut(s) 20, 271, 283, 338, 388, 545, 748, 1019, 1251
Alw21I GWGCWC 3 cut(s) 649, 668, 1111
Alw26I GTCTC 4 cut(s) 94, 1032, 1084, 1247
Alw44I GTGCAC 1 cut(s) 1107
AlwI GGATC 2 cut(s) 526, 1068
AoxI GGCC 4 cut(s) 55, 237, 684, 1123
ApaLI GTGCAC 1 cut(s) 1107
ApeKI GCWGC 3 cut(s) 839, 944, 1201
ApoI RAATTY 4 cut(s) 187, 372, 1178, 1225
AspA2I CCTAGG 1 cut(s) 775
AspLEI GCGC 1 cut(s) 605
AspS9I GGNCC 2 cut(s) 449, 685
AsuHPI GGTGA 3 cut(s) 764, 890, 1221
AvaII GGWCC 1 cut(s) 449
AvrII CCTAGG 1 cut(s) 775
BaeGI GKGCMC 2 cut(s) 479, 1111
BauI CACGAG 1 cut(s) 1236
BbrPI CACGTG 1 cut(s) 160
BbsI GAAGAC 1 cut(s) 804
Bbv12I GWGCWC 3 cut(s) 649, 668, 1111
BbvI GCAGC 3 cut(s) 826, 956, 1213
BceAI ACGGC 1 cut(s) 42
BciT130I CCWGG 1 cut(s) 516
BcoDI GTCTC 4 cut(s) 94, 1032, 1084, 1247
BfaI CTAG 3 cut(s) 21, 585, 776
BfmI CTRYAG 1 cut(s) 303
BisI GCNGC 3 cut(s) 840, 945, 1202
BlnI CCTAGG 1 cut(s) 775
BlsI GCNGC 3 cut(s) 841, 946, 1203
Bme1390I CCNGG 1 cut(s) 516
Bme18I GGWCC 1 cut(s) 449
BmeRI GACNNNNNGTC 1 cut(s) 1045
BmgT120I GGNCC 2 cut(s) 449, 685
BmiI GGNNCC 3 cut(s) 687, 766, 987
BmrFI CCNGG 1 cut(s) 516
BmrI ACTGGG 3 cut(s) 79, 592, 1083
BmsI GCATC 2 cut(s) 501, 559
BmuI ACTGGG 3 cut(s) 79, 592, 1083
BpiI GAAGAC 1 cut(s) 804
BplI GAGNNNNNCTC 2 cut(s) 74, 106
BpmI CTGGAG 1 cut(s) 920
Bpu10I CCTNAGC 1 cut(s) 284
BpuEI CTTGAG 1 cut(s) 1246
Bsa29I ATCGAT 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 592
BsaHI GRCGYC 1 cut(s) 51
BsaI GGTCTC 2 cut(s) 94, 1247
BsaJI CCNNGG 3 cut(s) 58, 775, 843
BsaWI WCCGGW 1 cut(s) 560
BsaXI ACNNNNNCTCC 2 cut(s) 726, 756
Bsc4I CCNNNNNNNGG 2 cut(s) 776, 1238
Bse1I ACTGG 6 cut(s) 74, 433, 452, 534, 598, 1089
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 592
BseBI CCWGG 1 cut(s) 516
BseCI ATCGAT 1 cut(s) 406
BseDI CCNNGG 3 cut(s) 58, 775, 843
BseGI GGATG 1 cut(s) 574
BseJI GATNNNNATC 1 cut(s) 592
BseLI CCNNNNNNNGG 2 cut(s) 776, 1238
BseMI GCAATG 1 cut(s) 552
BseMII CTCAG 2 cut(s) 597, 1011
BseNI ACTGG 6 cut(s) 74, 433, 452, 534, 598, 1089
BseRI GAGGAG 5 cut(s) 98, 163, 293, 750, 1224
BseSI GKGCMC 2 cut(s) 479, 1111
BseX3I CGGCCG 1 cut(s) 55
BseXI GCAGC 3 cut(s) 826, 956, 1213
Bsh1285I CGRYCG 1 cut(s) 58
BshFI GGCC 4 cut(s) 57, 239, 686, 1125
BshVI ATCGAT 1 cut(s) 406
BsiEI CGRYCG 1 cut(s) 58
BsiHKAI GWGCWC 3 cut(s) 649, 668, 1111
BsiSI CCGG 2 cut(s) 395, 561
BslFI GGGAC 3 cut(s) 87, 1077, 1133
BslI CCNNNNNNNGG 2 cut(s) 776, 1238
BsmAI GTCTC 4 cut(s) 94, 1032, 1084, 1247
BsmBI CGTCTC 1 cut(s) 1084
BsmFI GGGAC 3 cut(s) 87, 1077, 1133
BsmI GAATGC 1 cut(s) 221
BsnI GGCC 4 cut(s) 57, 239, 686, 1125
Bso31I GGTCTC 2 cut(s) 94, 1247
Bsp1286I GDGCHC 4 cut(s) 479, 649, 668, 1111
Bsp1407I TGTACA 1 cut(s) 345
Bsp143I GATC 5 cut(s) 469, 518, 679, 931, 1073
BspACI CCGC 5 cut(s) 9, 33, 39, 249, 1185
BspANI GGCC 4 cut(s) 57, 239, 686, 1125
BspCNI CTCAG 2 cut(s) 598, 1012
BspDI ATCGAT 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 687, 766, 987
BspPI GGATC 2 cut(s) 526, 1068
BspTNI GGTCTC 2 cut(s) 94, 1247
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 552
BsrGI TGTACA 1 cut(s) 345
BsrI ACTGG 6 cut(s) 74, 433, 452, 534, 598, 1089
BssECI CCNNGG 3 cut(s) 58, 775, 843
BssMI GATC 5 cut(s) 469, 518, 679, 931, 1073
BssNI GRCGYC 1 cut(s) 51
BssSI CACGAG 1 cut(s) 1236
BssT1I CCWWGG 1 cut(s) 775
Bst2BI CACGAG 1 cut(s) 1236
Bst2UI CCWGG 1 cut(s) 516
Bst4CI ACNGT 1 cut(s) 804
Bst6I CTCTTC 1 cut(s) 967
BstACI GRCGYC 1 cut(s) 51
BstAUI TGTACA 1 cut(s) 345
BstBAI YACGTR 1 cut(s) 160
BstC8I GCNNGC 3 cut(s) 49, 618, 902
BstDEI CTNAG 5 cut(s) 284, 606, 968, 1020, 1152
BstDSI CCRYGG 1 cut(s) 58
BstENI CCTNNNNNAGG 1 cut(s) 774
BstF5I GGATG 1 cut(s) 574
BstHHI GCGC 1 cut(s) 605
BstKTI GATC 5 cut(s) 472, 521, 682, 934, 1076
BstMAI GTCTC 4 cut(s) 94, 1032, 1084, 1247
BstMBI GATC 5 cut(s) 469, 518, 679, 931, 1073
BstMCI CGRYCG 1 cut(s) 58
BstMWI GCNNNNNNNGC 2 cut(s) 323, 1257
BstNI CCWGG 1 cut(s) 516
BstSCI CCNGG 1 cut(s) 514
BstSFI CTRYAG 1 cut(s) 303
BstSLI GKGCMC 2 cut(s) 479, 1111
BstV1I GCAGC 3 cut(s) 826, 956, 1213
BstV2I GAAGAC 1 cut(s) 804
BstX2I RGATCY 2 cut(s) 518, 1073
BstYI RGATCY 2 cut(s) 518, 1073
BstZI CGGCCG 1 cut(s) 55
Bsu15I ATCGAT 1 cut(s) 406
BsuRI GGCC 4 cut(s) 57, 239, 686, 1125
BsuTUI ATCGAT 1 cut(s) 406
BtgI CCRYGG 1 cut(s) 58
BtsCI GGATG 1 cut(s) 574
BtsI GCAGTG 1 cut(s) 987
BtsIMutI CAGTG 2 cut(s) 605, 987
Cac8I GCNNGC 3 cut(s) 49, 618, 902
CfoI GCGC 1 cut(s) 605
Cfr13I GGNCC 2 cut(s) 449, 685
ClaI ATCGAT 1 cut(s) 406
CseI GACGC 1 cut(s) 40
Csp6I GTAC 4 cut(s) 169, 330, 346, 1118
CviAII CATG 4 cut(s) 218, 241, 297, 892
CviQI GTAC 4 cut(s) 169, 330, 346, 1118
DdeI CTNAG 5 cut(s) 284, 606, 968, 1020, 1152
DpnI GATC 5 cut(s) 471, 520, 681, 933, 1075
DpnII GATC 5 cut(s) 469, 518, 679, 931, 1073
DraIII CACNNNGTG 1 cut(s) 1115
DriI GACNNNNNGTC 1 cut(s) 1045
EaeI YGGCCR 2 cut(s) 55, 1123
EagI CGGCCG 1 cut(s) 55
Eam1104I CTCTTC 1 cut(s) 967
Eam1105I GACNNNNNGTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 967
EciI GGCGGA 1 cut(s) 238
EclXI CGGCCG 1 cut(s) 55
Eco130I CCWWGG 1 cut(s) 775
Eco31I GGTCTC 2 cut(s) 94, 1247
Eco32I GATATC 1 cut(s) 915
Eco47I GGWCC 1 cut(s) 449
Eco52I CGGCCG 1 cut(s) 55
Eco72I CACGTG 1 cut(s) 160
EcoNI CCTNNNNNAGG 1 cut(s) 774
EcoRI GAATTC 2 cut(s) 187, 1225
EcoRII CCWGG 1 cut(s) 514
EcoRV GATATC 1 cut(s) 915
EcoT14I CCWWGG 1 cut(s) 775
EcoT22I ATGCAT 1 cut(s) 223
ErhI CCWWGG 1 cut(s) 775
Esp3I CGTCTC 1 cut(s) 1084
FaeI CATG 4 cut(s) 221, 244, 300, 895
FalI AAGNNNNNCTT 2 cut(s) 372, 404
FaqI GGGAC 3 cut(s) 87, 1077, 1133
FatI CATG 4 cut(s) 217, 240, 296, 891
FauI CCCGC 1 cut(s) 1178
Fnu4HI GCNGC 3 cut(s) 840, 945, 1202
FokI GGATG 1 cut(s) 581
Fsp4HI GCNGC 3 cut(s) 840, 945, 1202
FspBI CTAG 3 cut(s) 21, 585, 776
GlaI GCGC 1 cut(s) 604
GluI GCNGC 3 cut(s) 840, 945, 1202
GsuI CTGGAG 1 cut(s) 920
HaeIII GGCC 4 cut(s) 57, 239, 686, 1125
HapII CCGG 2 cut(s) 395, 561
HgaI GACGC 1 cut(s) 40
HhaI GCGC 1 cut(s) 605
Hin1I GRCGYC 1 cut(s) 51
Hin1II CATG 4 cut(s) 221, 244, 300, 895
Hin6I GCGC 1 cut(s) 603
HinP1I GCGC 1 cut(s) 603
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HindIII AAGCTT 3 cut(s) 336, 746, 1249
HinfI GANTC 9 cut(s) 63, 95, 105, 133, 154, 443, 593, 1024, 1040
HpaI GTTAAC 1 cut(s) 952
HpaII CCGG 2 cut(s) 395, 561
HphI GGTGA 3 cut(s) 764, 890, 1221
Hpy166II GTNNAC 4 cut(s) 722, 952, 1109, 1118
Hpy188I TCNGA 6 cut(s) 94, 360, 416, 670, 919, 963
Hpy188III TCNNGA 5 cut(s) 137, 230, 830, 937, 1263
Hpy8I GTNNAC 4 cut(s) 722, 952, 1109, 1118
Hpy99I CGWCG 2 cut(s) 56, 1260
HpyAV CCTTC 4 cut(s) 73, 374, 869, 890
HpyCH4III ACNGT 1 cut(s) 804
HpyCH4IV ACGT 3 cut(s) 159, 718, 1158
HpyCH4V TGCA 6 cut(s) 151, 221, 705, 904, 980, 1109
HpyF10VI GCNNNNNNNGC 2 cut(s) 323, 1257
HpyF3I CTNAG 5 cut(s) 284, 606, 968, 1020, 1152
HpySE526I ACGT 3 cut(s) 159, 718, 1158
Hsp92I GRCGYC 1 cut(s) 51
Hsp92II CATG 4 cut(s) 221, 244, 300, 895
HspAI GCGC 1 cut(s) 603
KspAI GTTAAC 1 cut(s) 952
Kzo9I GATC 5 cut(s) 469, 518, 679, 931, 1073
LmnI GCTCC 5 cut(s) 6, 280, 652, 770, 985
Lsp1109I GCAGC 3 cut(s) 826, 956, 1213
LweI GCATC 2 cut(s) 501, 559
MaeI CTAG 3 cut(s) 21, 585, 776
MaeII ACGT 3 cut(s) 159, 718, 1158
MaeIII GTNAC 1 cut(s) 817
MalI GATC 5 cut(s) 471, 520, 681, 933, 1075
MbiI CCGCTC 1 cut(s) 9
MboI GATC 5 cut(s) 469, 518, 679, 931, 1073
MboII GAAGA 5 cut(s) 109, 513, 804, 984, 994
MflI RGATCY 2 cut(s) 518, 1073
MhlI GDGCHC 4 cut(s) 479, 649, 668, 1111
MluCI AATT 8 cut(s) 187, 372, 637, 706, 755, 861, 1178, 1225
MlyI GAGTC 1 cut(s) 1034
MmeI TCCRAC 1 cut(s) 164
Mph1103I ATGCAT 1 cut(s) 223
MseI TTAA 5 cut(s) 291, 636, 864, 951, 1176
MslI CAYNNNNRTG 1 cut(s) 438
MspA1I CMGCKG 1 cut(s) 1019
MspI CCGG 2 cut(s) 395, 561
MspR9I CCNGG 1 cut(s) 516
Mva1269I GAATGC 1 cut(s) 221
MvaI CCWGG 1 cut(s) 516
MwoI GCNNNNNNNGC 2 cut(s) 323, 1257
NdeII GATC 5 cut(s) 469, 518, 679, 931, 1073
NlaIII CATG 4 cut(s) 221, 244, 300, 895
NlaIV GGNNCC 3 cut(s) 687, 766, 987
NmeAIII GCCGAG 1 cut(s) 1101
NsiI ATGCAT 1 cut(s) 223
PctI GAATGC 1 cut(s) 221
PfeI GAWTC 8 cut(s) 63, 95, 105, 133, 154, 443, 593, 1024
PkrI GCNGC 3 cut(s) 841, 946, 1203
PleI GAGTC 1 cut(s) 1034
PmaCI CACGTG 1 cut(s) 160
PmlI CACGTG 1 cut(s) 160
PpsI GAGTC 1 cut(s) 1034
Ppu21I YACGTR 1 cut(s) 160
PsiI TTATAA 1 cut(s) 786
Psp1406I AACGTT 2 cut(s) 718, 1158
Psp6I CCWGG 1 cut(s) 514
PspCI CACGTG 1 cut(s) 160
PspGI CCWGG 1 cut(s) 514
PspN4I GGNNCC 3 cut(s) 687, 766, 987
PspPI GGNCC 2 cut(s) 449, 685
PsuI RGATCY 2 cut(s) 518, 1073
PvuII CAGCTG 1 cut(s) 1019
RsaI GTAC 4 cut(s) 170, 331, 347, 1119
RsaNI GTAC 4 cut(s) 169, 330, 346, 1118
RseI CAYNNNNRTG 1 cut(s) 438
SaqAI TTAA 5 cut(s) 291, 636, 864, 951, 1176
SatI GCNGC 3 cut(s) 840, 945, 1202
Sau3AI GATC 5 cut(s) 469, 518, 679, 931, 1073
Sau96I GGNCC 2 cut(s) 449, 685
SchI GAGTC 1 cut(s) 1034
ScrFI CCNGG 1 cut(s) 516
SduI GDGCHC 4 cut(s) 479, 649, 668, 1111
SfaNI GCATC 2 cut(s) 501, 559
SfcI CTRYAG 1 cut(s) 303
SinI GGWCC 1 cut(s) 449
SmiMI CAYNNNNRTG 1 cut(s) 438
SmlI CTYRAG 1 cut(s) 1261
SmoI CTYRAG 1 cut(s) 1261
Sse9I AATT 8 cut(s) 187, 372, 637, 706, 755, 861, 1178, 1225
SsiI CCGC 5 cut(s) 9, 33, 39, 249, 1185
SspMI CTAG 3 cut(s) 21, 585, 776
StyD4I CCNGG 1 cut(s) 514
StyI CCWWGG 1 cut(s) 775
TaaI ACNGT 1 cut(s) 804
TaiI ACGT 3 cut(s) 162, 721, 1161
TaqI TCGA 2 cut(s) 131, 406
TasI AATT 8 cut(s) 187, 372, 637, 706, 755, 861, 1178, 1225
TatI WGTACW 3 cut(s) 168, 345, 1117
TfiI GAWTC 8 cut(s) 63, 95, 105, 133, 154, 443, 593, 1024
Tru1I TTAA 5 cut(s) 291, 636, 864, 951, 1176
Tru9I TTAA 5 cut(s) 291, 636, 864, 951, 1176
TscAI CASTG 2 cut(s) 605, 987
TseI GCWGC 3 cut(s) 839, 944, 1201
TspDTI ATGAA 2 cut(s) 618, 1074
TspGWI ACGGA 4 cut(s) 91, 384, 805, 1066
TspRI CASTG 2 cut(s) 605, 987
VneI GTGCAC 1 cut(s) 1107
VpaK11BI GGWCC 1 cut(s) 449
XagI CCTNNNNNAGG 1 cut(s) 774
XapI RAATTY 4 cut(s) 187, 372, 1178, 1225
XmaJI CCTAGG 1 cut(s) 775
XspI CTAG 3 cut(s) 21, 585, 776
Zsp2I ATGCAT 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.