Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000007

Orthogroup ID

OG0010502

Physical Location & Seq

Genomic Coordinates Reverse (-)

54902603 .. 54905922

Gene Structure 3320 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_7G00205350.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1242 bp

ATGTCGGCCGGTGCTGCTTCTGATCGCGGCGGCGGGATCTACTGGGGTCGAAAGGAGGTCTCCGATTCCAAAGGCATCGCCGTCATCTTCGCCTGGGTTTCGATTCCCGAGCGACACTTGAAGAGCTACGTCAATCTGTATGCGTCTCTGGGTTGGAACTCCCTCGTCTGCCATGCCCATTTTCTCCACGCATTCTATCCCGAAAGGGCGATGTCGTCGGCATTTGATATCCTGAATGAGCTTGTAGAGGAGCTAAGGATTAAGCCGTGTCCTGTAGTTTTTGTGGGTCTTTCTGCTGGTACTAAAGCTTGTATGTACAAAGTTTTTCAGATTATTGAAGGAATTAGTGAAGGCCAGCTATATCCGGGTGAATACCGATTGGTGAGGAAATGTATTACCGGACACATCTATGATTCCGGCCCGGTTGATTTTACAACTGACCTTGGTACACACTATGCTCTACACCCAACCATTCTAAAGATGCCTGGTTCATCAAAATTGGTTTCGTGGGTTGCTAAAGGCATTGCTTCTGGTTTGGATGCTCTTTATCTTACACGATTTGAATCCCAGTGGGCTGAGTATTGGCAGGCTCTGTATTCTTCAGTTAATTTGGGTGCTCCATTTCTCATTTTGTGCTCTGATAAAGACGAACTCGCTCCCTATCATGTTGTCTGCACTTTCAGTCAACGTTTACAAGAGTTTGGAGGTGACGTCAAGCTTGTGAAATTGAATGGTTCCCCTCACCTAGGTCATTATAAGAATTATCCAATACAATATAGGTCTTCTGTTACTCATTTTCTTGGGAAGGCAGCTTTTATTTTTTCTCAAAGAATTCAAAAGCTTGAATGGGAAATGACTAGCATGGAAGGTATGCATGATGAGATATCTGAACTAATCTGTGACCTCCAGAAAGCAGCCGTTAACTCTAATGAGAGCCTCAGAAGAGTTGCAGTTGAGCCAAGCGATCACTTCTTCTTGCCAAGTTCAGCAGAGAATCAAAATGGTACGGACTCTGGGTCATTACAAGATGAACAGAAAGAAAGATCGCTCTGTCTGTCTAAGCCTCCAAGCCTTAGTGCACATAGTGTACTTGGCAAAGTCCTATTTGATGTTTGTGTCCCTAAGAACGTTGATGGTTGGGATATCAAATTCTGTGGGTCTTTGAATGGGCAGCCATTTGCTTCTGCTCGTAGGAATTCACCTCCTCGTGGTATCAAAGGTCTCCGTCGCTCAAGATTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF829

Protein Analysis

413

Amino Acids

45.91

Weight (kDa)

7.98

Isoelectric Point (pI)

50.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF829	PF05705	27 - 101	4.8e-06	Eukaryotic protein of unknown function (DUF829)
DUF829	PF05705	132 - 380	5.8e-26	Eukaryotic protein of unknown function (DUF829)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010502)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G15695
fragaria_vesca	FvH4_2g06340 FvH4_2g06340
malus_domestica	MD05G1077400.v1.1 MD10G1092600.v1.1
prunus_persica	Prupe.8G122400_v2.0.a1
pyrus_communis	pycom05g07120 pycom10g07630
rosa_chinensis	RchiOBHm_Chr6g0258221
rosa_laevigata	RLG00000014638
rosa_multiflora	Rmu_sc0004230.1_g000007 Rmu_sc0006058.1_g000004
rosa_roxburghii	Rroxscaffold_7G00205350
rosa_rugosa	Rorug05G0579400 Rorug05G0579500
rosa_samantha	Rh6AG097400 Rh6BG088400 Rh6CG084300 Rh6DG080500
rosa_wichuraiana	Rw6G008250 Rw6G008330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	756
AatII	GACGTC	1 cut(s)	714
AccII	CGCG	1 cut(s)	27
AciI	CCGC	3 cut(s)	27, 30, 33
AclI	AACGTT	2 cut(s)	688, 1128
AclWI	GGATC	1 cut(s)	44
AcoI	YGGCCR	1 cut(s)	6
AcsI	RAATTY	3 cut(s)	831, 1148, 1195
AcuI	CTGAAG	1 cut(s)	585
AcyI	GRCGYC	1 cut(s)	711
AdeI	CACNNNGTG	1 cut(s)	1085
AfaI	GTAC	5 cut(s)	301, 317, 448, 1006, 1089
AfiI	CCNNNNNNNGG	2 cut(s)	746, 1208
AgsI	TTSAA	7 cut(s)	121, 338, 563, 730, 836, 845, 1165
AhdI	GACNNNNNGTC	1 cut(s)	1015
AjnI	CCWGG	2 cut(s)	92, 484
AluBI	AGCT	8 cut(s)	126, 241, 253, 308, 358, 718, 812, 841
AluI	AGCT	8 cut(s)	126, 241, 253, 308, 358, 718, 812, 841
Alw21I	GWGCWC	3 cut(s)	619, 638, 1081
Alw26I	GTCTC	3 cut(s)	64, 150, 1226
Alw44I	GTGCAC	1 cut(s)	1077
AlwI	GGATC	1 cut(s)	44
AlwNI	CAGNNNCTG	1 cut(s)	592
Ama87I	CYCGRG	1 cut(s)	107
AoxI	GGCC	3 cut(s)	6, 352, 418
ApaLI	GTGCAC	1 cut(s)	1077
ApeKI	GCWGC	4 cut(s)	14, 809, 914, 1171
ApoI	RAATTY	3 cut(s)	831, 1148, 1195
AspA2I	CCTAGG	1 cut(s)	745
AspS9I	GGNCC	1 cut(s)	419
AsuC2I	CCSGG	2 cut(s)	366, 422
AsuHPI	GGTGA	5 cut(s)	380, 394, 719, 734, 1191
AvaI	CYCGRG	1 cut(s)	107
AvrII	CCTAGG	1 cut(s)	745
BaeGI	GKGCMC	1 cut(s)	1081
BauI	CACGAG	1 cut(s)	1206
BbsI	GAAGAC	1 cut(s)	774
Bbv12I	GWGCWC	3 cut(s)	619, 638, 1081
BbvI	GCAGC	3 cut(s)	821, 926, 1183
BccI	CCATC	1 cut(s)	1127
BceAI	ACGGC	3 cut(s)	65, 250, 902
BciT130I	CCWGG	2 cut(s)	94, 486
BcnI	CCSGG	2 cut(s)	366, 422
BcoDI	GTCTC	3 cut(s)	64, 150, 1226
BfaI	CTAG	2 cut(s)	746, 858
BfmI	CTRYAG	1 cut(s)	273
BisI	GCNGC	6 cut(s)	15, 28, 31, 810, 915, 1172
BlnI	CCTAGG	1 cut(s)	745
BlsI	GCNGC	6 cut(s)	16, 29, 32, 811, 916, 1173
Bme1390I	CCNGG	4 cut(s)	94, 366, 422, 486
BmeRI	GACNNNNNGTC	1 cut(s)	1015
BmeT110I	CYCGRG	1 cut(s)	107
BmgT120I	GGNCC	1 cut(s)	419
BmiI	GGNNCC	1 cut(s)	736
BmrFI	CCNGG	4 cut(s)	94, 366, 422, 486
BmrI	ACTGGG	2 cut(s)	52, 562
BmsI	GCATC	3 cut(s)	84, 471, 529
BmuI	ACTGGG	2 cut(s)	52, 562
BpiI	GAAGAC	1 cut(s)	774
BpmI	CTGGAG	1 cut(s)	890
Bpu10I	CCTNAGC	1 cut(s)	254
BpuEI	CTTGAG	1 cut(s)	1216
BpuMI	CCSGG	2 cut(s)	366, 422
BsaBI	GATNNNNATC	1 cut(s)	562
BsaHI	GRCGYC	1 cut(s)	711
BsaI	GGTCTC	2 cut(s)	64, 1226
BsaJI	CCNNGG	3 cut(s)	93, 442, 745
BsaWI	WCCGGW	1 cut(s)	398
BsaXI	ACNNNNNCTCC	2 cut(s)	696, 726
Bsc4I	CCNNNNNNNGG	2 cut(s)	746, 1208
Bse118I	RCCGGY	1 cut(s)	8
Bse1I	ACTGG	2 cut(s)	47, 568
Bse3DI	GCAATG	1 cut(s)	522
Bse8I	GATNNNNATC	1 cut(s)	562
BseBI	CCWGG	2 cut(s)	94, 486
BseDI	CCNNGG	3 cut(s)	93, 442, 745
BseGI	GGATG	1 cut(s)	544
BseJI	GATNNNNATC	1 cut(s)	562
BseLI	CCNNNNNNNGG	2 cut(s)	746, 1208
BseMI	GCAATG	1 cut(s)	522
BseMII	CTCAG	2 cut(s)	567, 952
BseNI	ACTGG	2 cut(s)	47, 568
BseRI	GAGGAG	2 cut(s)	263, 1194
BseSI	GKGCMC	1 cut(s)	1081
BseX3I	CGGCCG	1 cut(s)	6
BseXI	GCAGC	3 cut(s)	821, 926, 1183
BsgI	GTGCAG	1 cut(s)	658
Bsh1236I	CGCG	1 cut(s)	27
Bsh1285I	CGRYCG	1 cut(s)	9
BshFI	GGCC	3 cut(s)	8, 354, 420
BsiEI	CGRYCG	1 cut(s)	9
BsiHKAI	GWGCWC	3 cut(s)	619, 638, 1081
BsiHKCI	CYCGRG	1 cut(s)	107
BsiSI	CCGG	5 cut(s)	9, 365, 399, 417, 422
BslFI	GGGAC	1 cut(s)	1103
BslI	CCNNNNNNNGG	2 cut(s)	746, 1208
BsmAI	GTCTC	3 cut(s)	64, 150, 1226
BsmBI	CGTCTC	1 cut(s)	150
BsmFI	GGGAC	1 cut(s)	1103
BsmI	GAATGC	1 cut(s)	191
BsnI	GGCC	3 cut(s)	8, 354, 420
Bso31I	GGTCTC	2 cut(s)	64, 1226
BsoBI	CYCGRG	1 cut(s)	107
Bsp1286I	GDGCHC	3 cut(s)	619, 638, 1081
Bsp1407I	TGTACA	1 cut(s)	315
Bsp143I	GATC	4 cut(s)	22, 36, 964, 1043
BspACI	CCGC	3 cut(s)	27, 30, 33
BspANI	GGCC	3 cut(s)	8, 354, 420
BspCNI	CTCAG	2 cut(s)	568, 951
BspFNI	CGCG	1 cut(s)	27
BspLI	GGNNCC	1 cut(s)	736
BspPI	GGATC	1 cut(s)	44
BspQI	GCTCTTC	1 cut(s)	116
BspTNI	GGTCTC	2 cut(s)	64, 1226
BsrDI	GCAATG	1 cut(s)	522
BsrFI	RCCGGY	1 cut(s)	8
BsrGI	TGTACA	1 cut(s)	315
BsrI	ACTGG	2 cut(s)	47, 568
BssAI	RCCGGY	1 cut(s)	8
BssECI	CCNNGG	3 cut(s)	93, 442, 745
BssMI	GATC	4 cut(s)	22, 36, 964, 1043
BssNI	GRCGYC	1 cut(s)	711
BssSI	CACGAG	1 cut(s)	1206
BssT1I	CCWWGG	2 cut(s)	442, 745
Bst2BI	CACGAG	1 cut(s)	1206
Bst2UI	CCWGG	2 cut(s)	94, 486
Bst6I	CTCTTC	2 cut(s)	116, 937
BstACI	GRCGYC	1 cut(s)	711
BstAUI	TGTACA	1 cut(s)	315
BstC8I	GCNNGC	2 cut(s)	356, 588
BstDEI	CTNAG	6 cut(s)	254, 576, 938, 1059, 1073, 1122
BstENI	CCTNNNNNAGG	1 cut(s)	744
BstF5I	GGATG	1 cut(s)	544
BstFNI	CGCG	1 cut(s)	27
BstKTI	GATC	4 cut(s)	25, 39, 967, 1046
BstMAI	GTCTC	3 cut(s)	64, 150, 1226
BstMBI	GATC	4 cut(s)	22, 36, 964, 1043
BstMCI	CGRYCG	1 cut(s)	9
BstMWI	GCNNNNNNNGC	1 cut(s)	14
BstNI	CCWGG	2 cut(s)	94, 486
BstSCI	CCNGG	4 cut(s)	92, 364, 420, 484
BstSFI	CTRYAG	1 cut(s)	273
BstSLI	GKGCMC	1 cut(s)	1081
BstUI	CGCG	1 cut(s)	27
BstV1I	GCAGC	3 cut(s)	821, 926, 1183
BstV2I	GAAGAC	1 cut(s)	774
BstX2I	RGATCY	1 cut(s)	36
BstYI	RGATCY	1 cut(s)	36
BstZI	CGGCCG	1 cut(s)	6
BsuRI	GGCC	3 cut(s)	8, 354, 420
BtgZI	GCGATG	2 cut(s)	61, 224
BtsCI	GGATG	1 cut(s)	544
BtsIMutI	CAGTG	1 cut(s)	575
Cac8I	GCNNGC	2 cut(s)	356, 588
CaiI	CAGNNNCTG	1 cut(s)	592
Cfr10I	RCCGGY	1 cut(s)	8
Cfr13I	GGNCC	1 cut(s)	419
CseI	GACGC	1 cut(s)	132
Csp6I	GTAC	5 cut(s)	300, 316, 447, 1005, 1088
CspCI	CAANNNNNGTGG	2 cut(s)	1135, 1170
CviAII	CATG	4 cut(s)	173, 665, 862, 875
CviQI	GTAC	5 cut(s)	300, 316, 447, 1005, 1088
DdeI	CTNAG	6 cut(s)	254, 576, 938, 1059, 1073, 1122
DpnI	GATC	4 cut(s)	24, 38, 966, 1045
DpnII	GATC	4 cut(s)	22, 36, 964, 1043
DraIII	CACNNNGTG	1 cut(s)	1085
DriI	GACNNNNNGTC	1 cut(s)	1015
EaeI	YGGCCR	1 cut(s)	6
EagI	CGGCCG	1 cut(s)	6
Eam1104I	CTCTTC	2 cut(s)	116, 937
Eam1105I	GACNNNNNGTC	1 cut(s)	1015
EarI	CTCTTC	2 cut(s)	116, 937
EclXI	CGGCCG	1 cut(s)	6
Eco130I	CCWWGG	2 cut(s)	442, 745
Eco31I	GGTCTC	2 cut(s)	64, 1226
Eco32I	GATATC	3 cut(s)	229, 885, 1144
Eco52I	CGGCCG	1 cut(s)	6
Eco57I	CTGAAG	1 cut(s)	585
Eco88I	CYCGRG	1 cut(s)	107
EcoNI	CCTNNNNNAGG	1 cut(s)	744
EcoRI	GAATTC	2 cut(s)	831, 1195
EcoRII	CCWGG	2 cut(s)	92, 484
EcoRV	GATATC	3 cut(s)	229, 885, 1144
EcoT14I	CCWWGG	2 cut(s)	442, 745
EcoT22I	ATGCAT	1 cut(s)	876
ErhI	CCWWGG	2 cut(s)	442, 745
Esp3I	CGTCTC	1 cut(s)	150
FaeI	CATG	4 cut(s)	176, 668, 865, 878
FaqI	GGGAC	1 cut(s)	1103
FatI	CATG	4 cut(s)	172, 664, 861, 874
FauI	CCCGC	1 cut(s)	26
Fnu4HI	GCNGC	6 cut(s)	15, 28, 31, 810, 915, 1172
FokI	GGATG	1 cut(s)	551
Fsp4HI	GCNGC	6 cut(s)	15, 28, 31, 810, 915, 1172
FspBI	CTAG	2 cut(s)	746, 858
GluI	GCNGC	6 cut(s)	15, 28, 31, 810, 915, 1172
GsuI	CTGGAG	1 cut(s)	890
HaeIII	GGCC	3 cut(s)	8, 354, 420
HapII	CCGG	5 cut(s)	9, 365, 399, 417, 422
HgaI	GACGC	1 cut(s)	132
Hin1I	GRCGYC	1 cut(s)	711
Hin1II	CATG	4 cut(s)	176, 668, 865, 878
HincII	GTYRAC	2 cut(s)	686, 922
HindII	GTYRAC	2 cut(s)	686, 922
HindIII	AAGCTT	3 cut(s)	306, 716, 839
HinfI	GANTC	6 cut(s)	65, 103, 413, 563, 994, 1010
HpaI	GTTAAC	1 cut(s)	922
HpaII	CCGG	5 cut(s)	9, 365, 399, 417, 422
HphI	GGTGA	5 cut(s)	380, 394, 719, 734, 1191
Hpy166II	GTNNAC	6 cut(s)	449, 686, 692, 922, 1079, 1088
Hpy188I	TCNGA	6 cut(s)	22, 64, 330, 640, 889, 941
Hpy188III	TCNNGA	5 cut(s)	107, 200, 232, 907, 1233
Hpy8I	GTNNAC	6 cut(s)	449, 686, 692, 922, 1079, 1088
Hpy99I	CGWCG	2 cut(s)	220, 1230
HpyAV	CCTTC	4 cut(s)	332, 344, 799, 860
HpyCH4IV	ACGT	4 cut(s)	129, 688, 711, 1128
HpyCH4V	TGCA	4 cut(s)	675, 874, 950, 1079
HpyF10VI	GCNNNNNNNGC	1 cut(s)	14
HpyF3I	CTNAG	6 cut(s)	254, 576, 938, 1059, 1073, 1122
HpySE526I	ACGT	4 cut(s)	129, 688, 711, 1128
Hsp92I	GRCGYC	1 cut(s)	711
Hsp92II	CATG	4 cut(s)	176, 668, 865, 878
KspAI	GTTAAC	1 cut(s)	922
Kzo9I	GATC	4 cut(s)	22, 36, 964, 1043
LguI	GCTCTTC	1 cut(s)	116
LmnI	GCTCC	3 cut(s)	250, 622, 661
Lsp1109I	GCAGC	3 cut(s)	821, 926, 1183
LweI	GCATC	3 cut(s)	84, 471, 529
MaeI	CTAG	2 cut(s)	746, 858
MaeII	ACGT	4 cut(s)	129, 688, 711, 1128
MaeIII	GTNAC	3 cut(s)	707, 787, 899
MalI	GATC	4 cut(s)	24, 38, 966, 1045
MboI	GATC	4 cut(s)	22, 36, 964, 1043
MboII	GAAGA	6 cut(s)	79, 133, 591, 774, 954, 964
MflI	RGATCY	1 cut(s)	36
MhlI	GDGCHC	3 cut(s)	619, 638, 1081
MluCI	AATT	8 cut(s)	342, 497, 607, 725, 760, 831, 1148, 1195
MlyI	GAGTC	1 cut(s)	1004
MmeI	TCCRAC	1 cut(s)	134
Mph1103I	ATGCAT	1 cut(s)	876
MseI	TTAA	3 cut(s)	261, 606, 921
MslI	CAYNNNNRTG	1 cut(s)	408
MspI	CCGG	5 cut(s)	9, 365, 399, 417, 422
MspR9I	CCNGG	4 cut(s)	94, 366, 422, 486
Mva1269I	GAATGC	1 cut(s)	191
MvaI	CCWGG	2 cut(s)	94, 486
MvnI	CGCG	1 cut(s)	27
MwoI	GCNNNNNNNGC	1 cut(s)	14
NciI	CCSGG	2 cut(s)	366, 422
NdeII	GATC	4 cut(s)	22, 36, 964, 1043
NlaIII	CATG	4 cut(s)	176, 668, 865, 878
NlaIV	GGNNCC	1 cut(s)	736
NmuCI	GTSAC	2 cut(s)	707, 899
NsiI	ATGCAT	1 cut(s)	876
PciSI	GCTCTTC	1 cut(s)	116
PctI	GAATGC	1 cut(s)	191
PfeI	GAWTC	5 cut(s)	65, 103, 413, 563, 994
PkrI	GCNGC	6 cut(s)	16, 29, 32, 811, 916, 1173
PleI	GAGTC	1 cut(s)	1004
PpsI	GAGTC	1 cut(s)	1004
PsiI	TTATAA	1 cut(s)	756
Psp1406I	AACGTT	2 cut(s)	688, 1128
Psp6I	CCWGG	2 cut(s)	92, 484
PspGI	CCWGG	2 cut(s)	92, 484
PspN4I	GGNNCC	1 cut(s)	736
PspPI	GGNCC	1 cut(s)	419
PstNI	CAGNNNCTG	1 cut(s)	592
PsuI	RGATCY	1 cut(s)	36
RsaI	GTAC	5 cut(s)	301, 317, 448, 1006, 1089
RsaNI	GTAC	5 cut(s)	300, 316, 447, 1005, 1088
RseI	CAYNNNNRTG	1 cut(s)	408
SapI	GCTCTTC	1 cut(s)	116
SaqAI	TTAA	3 cut(s)	261, 606, 921
SatI	GCNGC	6 cut(s)	15, 28, 31, 810, 915, 1172
Sau3AI	GATC	4 cut(s)	22, 36, 964, 1043
Sau96I	GGNCC	1 cut(s)	419
SchI	GAGTC	1 cut(s)	1004
ScrFI	CCNGG	4 cut(s)	94, 366, 422, 486
SduI	GDGCHC	3 cut(s)	619, 638, 1081
SfaNI	GCATC	3 cut(s)	84, 471, 529
SfcI	CTRYAG	1 cut(s)	273
SmiMI	CAYNNNNRTG	1 cut(s)	408
SmlI	CTYRAG	1 cut(s)	1231
SmoI	CTYRAG	1 cut(s)	1231
Sse9I	AATT	8 cut(s)	342, 497, 607, 725, 760, 831, 1148, 1195
SsiI	CCGC	3 cut(s)	27, 30, 33
SspMI	CTAG	2 cut(s)	746, 858
StyD4I	CCNGG	4 cut(s)	92, 364, 420, 484
StyI	CCWWGG	2 cut(s)	442, 745
TaiI	ACGT	4 cut(s)	132, 691, 714, 1131
TaqI	TCGA	2 cut(s)	49, 101
TasI	AATT	8 cut(s)	342, 497, 607, 725, 760, 831, 1148, 1195
TatI	WGTACW	2 cut(s)	315, 1087
TauI	GCSGC	2 cut(s)	30, 33
TfiI	GAWTC	5 cut(s)	65, 103, 413, 563, 994
Tru1I	TTAA	3 cut(s)	261, 606, 921
Tru9I	TTAA	3 cut(s)	261, 606, 921
TscAI	CASTG	1 cut(s)	575
TseFI	GTSAC	2 cut(s)	707, 899
TseI	GCWGC	4 cut(s)	14, 809, 914, 1171
Tsp45I	GTSAC	2 cut(s)	707, 899
TspDTI	ATGAA	2 cut(s)	480, 1044
TspGWI	ACGGA	2 cut(s)	1022, 1214
TspRI	CASTG	1 cut(s)	575
VneI	GTGCAC	1 cut(s)	1077
XagI	CCTNNNNNAGG	1 cut(s)	744
XapI	RAATTY	3 cut(s)	831, 1148, 1195
XmaJI	CCTAGG	1 cut(s)	745
XspI	CTAG	2 cut(s)	746, 858
ZraI	GACGTC	1 cut(s)	712
Zsp2I	ATGCAT	1 cut(s)	876

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.