RLG00000014638

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0010502
Physical Location & Seq
Forward (+)
55627064 .. 55629772
2709 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014638

Sequence Viewer

Length: 1245 bp
ATGTCGGGCGCCGGTGCTGCTTCTGATCGCGGCGGCGGGATCTACTGGGGTCGAAAGGAGGTCTCCGATTCCAAAGGCATCGCCGTCATCTTCGCCTGGGTTTCGATTCCCGAGCGACACTTGAAGAGCTACGTCAATCTGTACGCGTCTCTGGGTTGGAACTCCCTCGTCTGCCATGCCCATTTTCTCCACGCATTCTATCCCGAAAGGGCGATGTCGTCGGCATTTGATATCCTGAATGAGCTTGTGGAGGAGCTAAGGATTAAGCCGTGTCCCGTTGTTTTTGTGGGTCTTTCTGCTGGTACTAAAGCTTGTATGTACAAAGTTTTTCAGATTATTGAAGGAATTTGCGAAGGCCAGCTGTATCCGGGTGAATACCGATTGGTGAGGAAATGTATTACCGGACACATCTATGATTCCGGCCCGGTTGATTTTACAACTGACCTTGGTACACACTATGCTCTACACCCAACCATTCTGAAGATGCCTGGTTCATCAAAATTGGTTTCGTGGGTTGCTAAAGGCATTGCTTCTGGTTTGGATGCTCTTTATCTTACGCGATTTGAATCCCAGTGGGCTGAGTATTGGCAGGCTCTGTATTCTTCAGTTAATTTGGGTGCTCCATTTCTCATTTTGTGCTCTGATAAAGATGATCTCGCTCCCTATCATGTCGTCTGCACTTTCAGTCAACGTTTACAAGAGTTTGGAGGTGATGTCAAGCTTGTGAAATTGAATGGTTCCCCTCACCTAGGTCATTATAAGAATTATCCAATACAATATAGGTCTTCTGTTACTCATTTTCTTGGGAAGGCAGCTTTGGTTTTTTCTCAAAGAATTCAAAAGCTTGAATGGGAAATGACTAGCATGGAAGGCATGCATGATGAGATATCTGAACTAATCTGTGATCTCCAGAAAGCAGCCGTTAACTCTAATGAGAGCCTCAGAAGAGTTGCAGTTGAGCCAAGTGATCACTTCTTCTTGCCAAGTTCCGCAGAGAATCAAAATGGTACTGACTCTGGGTCATTACAAGATGAACAGAAAGAAAGATCACCCTGTCTGTCTAATCCTCCGAGCCTTAGTGCACACAGTGTACTTGGCAAAGTCCTGTTTGATGTTTGTGTCCCTAAGAACGTGGATGGTTGGGATATCAAATTCTGTGGGTCTTTGAATGGGCAGCCATTTGCTTCTGCTCGTAGGAATTCACCTCCTCGTGGTATCAAAGGTCTCCGTCGCTCGAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

415

Amino Acids

45.89

Weight (kDa)

7.59

Isoelectric Point (pI)

50.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 28 - 103 4.8e-06 Eukaryotic protein of unknown function (DUF829)
DUF829 PF05705 133 - 381 1.7e-25 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 759
AccB1I GGYRCC 1 cut(s) 8
AccII CGCG 3 cut(s) 30, 146, 559
AciI CCGC 4 cut(s) 30, 33, 36, 990
AclI AACGTT 1 cut(s) 691
AclWI GGATC 1 cut(s) 47
AcsI RAATTY 4 cut(s) 345, 834, 1151, 1198
AcuI CTGAAG 2 cut(s) 500, 588
AcyI GRCGYC 1 cut(s) 9
AdeI CACNNNGTG 1 cut(s) 1088
AfaI GTAC 6 cut(s) 143, 304, 320, 451, 1009, 1092
AfiI CCNNNNNNNGG 2 cut(s) 749, 1211
AflIII ACRYGT 1 cut(s) 144
AgsI TTSAA 7 cut(s) 124, 341, 566, 733, 839, 848, 1168
AhdI GACNNNNNGTC 1 cut(s) 1018
AjnI CCWGG 2 cut(s) 95, 487
AjuI GAANNNNNNNTTGG 2 cut(s) 800, 832
AluBI AGCT 8 cut(s) 129, 244, 256, 311, 361, 721, 815, 844
AluI AGCT 8 cut(s) 129, 244, 256, 311, 361, 721, 815, 844
Alw21I GWGCWC 3 cut(s) 622, 641, 1084
Alw26I GTCTC 3 cut(s) 67, 153, 1229
Alw44I GTGCAC 1 cut(s) 1080
AlwI GGATC 1 cut(s) 47
AlwNI CAGNNNCTG 1 cut(s) 595
Ama87I CYCGRG 2 cut(s) 110, 1234
AoxI GGCC 2 cut(s) 355, 421
ApaLI GTGCAC 1 cut(s) 1080
ApeKI GCWGC 4 cut(s) 17, 812, 917, 1174
ApoI RAATTY 4 cut(s) 345, 834, 1151, 1198
AspA2I CCTAGG 1 cut(s) 748
AspLEI GCGC 1 cut(s) 11
AspS9I GGNCC 1 cut(s) 422
AsuC2I CCSGG 2 cut(s) 369, 425
AsuHPI GGTGA 6 cut(s) 383, 397, 722, 737, 1041, 1194
AvaI CYCGRG 2 cut(s) 110, 1234
AvrII CCTAGG 1 cut(s) 748
BaeGI GKGCMC 1 cut(s) 1084
BanI GGYRCC 1 cut(s) 8
BauI CACGAG 1 cut(s) 1209
BbsI GAAGAC 1 cut(s) 777
Bbv12I GWGCWC 3 cut(s) 622, 641, 1084
BbvI GCAGC 4 cut(s) 4, 824, 929, 1186
BccI CCATC 1 cut(s) 1130
BceAI ACGGC 3 cut(s) 68, 253, 905
BciT130I CCWGG 2 cut(s) 97, 489
BciVI GTATCC 1 cut(s) 375
BclI TGATCA 1 cut(s) 967
BcnI CCSGG 2 cut(s) 369, 425
BcoDI GTCTC 3 cut(s) 67, 153, 1229
BfaI CTAG 2 cut(s) 749, 861
BfoI RGCGCY 1 cut(s) 12
BfuI GTATCC 1 cut(s) 375
BisI GCNGC 6 cut(s) 18, 31, 34, 813, 918, 1175
BlnI CCTAGG 1 cut(s) 748
BlsI GCNGC 6 cut(s) 19, 32, 35, 814, 919, 1176
Bme1390I CCNGG 4 cut(s) 97, 369, 425, 489
BmeRI GACNNNNNGTC 1 cut(s) 1018
BmeT110I CYCGRG 2 cut(s) 110, 1234
BmgT120I GGNCC 1 cut(s) 422
BmiI GGNNCC 2 cut(s) 10, 739
BmrFI CCNGG 4 cut(s) 97, 369, 425, 489
BmrI ACTGGG 2 cut(s) 55, 565
BmsI GCATC 3 cut(s) 87, 474, 532
BmuI ACTGGG 2 cut(s) 55, 565
BpiI GAAGAC 1 cut(s) 777
BpmI CTGGAG 1 cut(s) 893
Bpu10I CCTNAGC 1 cut(s) 257
BpuMI CCSGG 2 cut(s) 369, 425
BsaBI GATNNNNATC 1 cut(s) 565
BsaHI GRCGYC 1 cut(s) 9
BsaI GGTCTC 2 cut(s) 67, 1229
BsaJI CCNNGG 3 cut(s) 96, 445, 748
BsaWI WCCGGW 1 cut(s) 401
BsaXI ACNNNNNCTCC 2 cut(s) 699, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 749, 1211
Bse118I RCCGGY 1 cut(s) 11
Bse1I ACTGG 2 cut(s) 50, 571
Bse3DI GCAATG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 565
BseBI CCWGG 2 cut(s) 97, 489
BseDI CCNNGG 3 cut(s) 96, 445, 748
BseGI GGATG 2 cut(s) 547, 1141
BseJI GATNNNNATC 1 cut(s) 565
BseLI CCNNNNNNNGG 2 cut(s) 749, 1211
BseMI GCAATG 1 cut(s) 525
BseMII CTCAG 2 cut(s) 570, 955
BseNI ACTGG 2 cut(s) 50, 571
BseRI GAGGAG 2 cut(s) 266, 1197
BseSI GKGCMC 1 cut(s) 1084
BseXI GCAGC 4 cut(s) 4, 824, 929, 1186
BsgI GTGCAG 1 cut(s) 661
Bsh1236I CGCG 3 cut(s) 30, 146, 559
BshFI GGCC 2 cut(s) 357, 423
BshNI GGYRCC 1 cut(s) 8
BsiHKAI GWGCWC 3 cut(s) 622, 641, 1084
BsiHKCI CYCGRG 2 cut(s) 110, 1234
BsiSI CCGG 5 cut(s) 12, 368, 402, 420, 425
BslFI GGGAC 2 cut(s) 258, 1106
BslI CCNNNNNNNGG 2 cut(s) 749, 1211
BsmAI GTCTC 3 cut(s) 67, 153, 1229
BsmBI CGTCTC 1 cut(s) 153
BsmFI GGGAC 2 cut(s) 258, 1106
BsmI GAATGC 1 cut(s) 194
BsnI GGCC 2 cut(s) 357, 423
Bso31I GGTCTC 2 cut(s) 67, 1229
BsoBI CYCGRG 2 cut(s) 110, 1234
Bsp1286I GDGCHC 3 cut(s) 622, 641, 1084
Bsp1407I TGTACA 1 cut(s) 318
Bsp143I GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
BspACI CCGC 4 cut(s) 30, 33, 36, 990
BspANI GGCC 2 cut(s) 357, 423
BspCNI CTCAG 2 cut(s) 571, 954
BspFNI CGCG 3 cut(s) 30, 146, 559
BspLI GGNNCC 2 cut(s) 10, 739
BspPI GGATC 1 cut(s) 47
BspQI GCTCTTC 1 cut(s) 119
BspT107I GGYRCC 1 cut(s) 8
BspTNI GGTCTC 2 cut(s) 67, 1229
BsrDI GCAATG 1 cut(s) 525
BsrFI RCCGGY 1 cut(s) 11
BsrGI TGTACA 1 cut(s) 318
BsrI ACTGG 2 cut(s) 50, 571
BssAI RCCGGY 1 cut(s) 11
BssECI CCNNGG 3 cut(s) 96, 445, 748
BssMI GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
BssNI GRCGYC 1 cut(s) 9
BssSI CACGAG 1 cut(s) 1209
BssT1I CCWWGG 2 cut(s) 445, 748
Bst2BI CACGAG 1 cut(s) 1209
Bst2UI CCWGG 2 cut(s) 97, 489
Bst4CI ACNGT 1 cut(s) 1088
Bst6I CTCTTC 2 cut(s) 119, 940
BstACI GRCGYC 1 cut(s) 9
BstAUI TGTACA 1 cut(s) 318
BstC8I GCNNGC 3 cut(s) 359, 591, 875
BstDEI CTNAG 5 cut(s) 257, 579, 941, 1076, 1125
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 2 cut(s) 547, 1141
BstFNI CGCG 3 cut(s) 30, 146, 559
BstH2I RGCGCY 1 cut(s) 12
BstHHI GCGC 1 cut(s) 11
BstKTI GATC 6 cut(s) 28, 42, 655, 907, 970, 1049
BstMAI GTCTC 3 cut(s) 67, 153, 1229
BstMBI GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
BstMWI GCNNNNNNNGC 2 cut(s) 17, 870
BstNI CCWGG 2 cut(s) 97, 489
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 4 cut(s) 95, 367, 423, 487
BstSLI GKGCMC 1 cut(s) 1084
BstUI CGCG 3 cut(s) 30, 146, 559
BstV1I GCAGC 4 cut(s) 4, 824, 929, 1186
BstV2I GAAGAC 1 cut(s) 777
BstX2I RGATCY 1 cut(s) 39
BstYI RGATCY 1 cut(s) 39
BsuI GTATCC 1 cut(s) 375
BsuRI GGCC 2 cut(s) 357, 423
BtgZI GCGATG 2 cut(s) 64, 227
BtsCI GGATG 2 cut(s) 547, 1141
BtsIMutI CAGTG 2 cut(s) 578, 1093
Cac8I GCNNGC 3 cut(s) 359, 591, 875
CaiI CAGNNNCTG 1 cut(s) 595
CfoI GCGC 1 cut(s) 11
Cfr10I RCCGGY 1 cut(s) 11
Cfr13I GGNCC 1 cut(s) 422
CseI GACGC 1 cut(s) 135
Csp6I GTAC 6 cut(s) 142, 303, 319, 450, 1008, 1091
CspCI CAANNNNNGTGG 2 cut(s) 1138, 1173
CviAII CATG 5 cut(s) 176, 668, 865, 874, 878
CviQI GTAC 6 cut(s) 142, 303, 319, 450, 1008, 1091
DdeI CTNAG 5 cut(s) 257, 579, 941, 1076, 1125
DinI GGCGCC 1 cut(s) 10
DpnI GATC 6 cut(s) 27, 41, 654, 906, 969, 1048
DpnII GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
DraIII CACNNNGTG 1 cut(s) 1088
DriI GACNNNNNGTC 1 cut(s) 1018
Eam1104I CTCTTC 2 cut(s) 119, 940
Eam1105I GACNNNNNGTC 1 cut(s) 1018
EarI CTCTTC 2 cut(s) 119, 940
Eco130I CCWWGG 2 cut(s) 445, 748
Eco31I GGTCTC 2 cut(s) 67, 1229
Eco32I GATATC 3 cut(s) 232, 888, 1147
Eco57I CTGAAG 2 cut(s) 500, 588
Eco88I CYCGRG 2 cut(s) 110, 1234
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoRI GAATTC 2 cut(s) 834, 1198
EcoRII CCWGG 2 cut(s) 95, 487
EcoRV GATATC 3 cut(s) 232, 888, 1147
EcoT14I CCWWGG 2 cut(s) 445, 748
EcoT22I ATGCAT 1 cut(s) 879
EgeI GGCGCC 1 cut(s) 10
EheI GGCGCC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 445, 748
Esp3I CGTCTC 1 cut(s) 153
FaeI CATG 5 cut(s) 179, 671, 868, 877, 881
FaqI GGGAC 2 cut(s) 258, 1106
FatI CATG 5 cut(s) 175, 667, 864, 873, 877
FauI CCCGC 1 cut(s) 29
FbaI TGATCA 1 cut(s) 967
Fnu4HI GCNGC 6 cut(s) 18, 31, 34, 813, 918, 1175
FokI GGATG 2 cut(s) 554, 1148
Fsp4HI GCNGC 6 cut(s) 18, 31, 34, 813, 918, 1175
FspBI CTAG 2 cut(s) 749, 861
GlaI GCGC 1 cut(s) 10
GluI GCNGC 6 cut(s) 18, 31, 34, 813, 918, 1175
GsuI CTGGAG 1 cut(s) 893
HaeII RGCGCY 1 cut(s) 12
HaeIII GGCC 2 cut(s) 357, 423
HapII CCGG 5 cut(s) 12, 368, 402, 420, 425
HgaI GACGC 1 cut(s) 135
HhaI GCGC 1 cut(s) 11
Hin1I GRCGYC 1 cut(s) 9
Hin1II CATG 5 cut(s) 179, 671, 868, 877, 881
Hin6I GCGC 1 cut(s) 9
HinP1I GCGC 1 cut(s) 9
HincII GTYRAC 2 cut(s) 689, 925
HindII GTYRAC 2 cut(s) 689, 925
HindIII AAGCTT 3 cut(s) 309, 719, 842
HinfI GANTC 6 cut(s) 68, 106, 416, 566, 997, 1013
HpaI GTTAAC 1 cut(s) 925
HpaII CCGG 5 cut(s) 12, 368, 402, 420, 425
HphI GGTGA 6 cut(s) 383, 397, 722, 737, 1041, 1194
Hpy166II GTNNAC 6 cut(s) 452, 689, 695, 925, 1082, 1091
Hpy188I TCNGA 8 cut(s) 25, 67, 333, 480, 643, 892, 944, 1071
Hpy188III TCNNGA 5 cut(s) 110, 203, 235, 910, 1236
Hpy8I GTNNAC 6 cut(s) 452, 689, 695, 925, 1082, 1091
Hpy99I CGWCG 2 cut(s) 223, 1233
HpyAV CCTTC 4 cut(s) 335, 347, 802, 863
HpyCH4III ACNGT 1 cut(s) 1088
HpyCH4IV ACGT 3 cut(s) 132, 691, 1131
HpyCH4V TGCA 4 cut(s) 678, 877, 953, 1082
HpyF10VI GCNNNNNNNGC 2 cut(s) 17, 870
HpyF3I CTNAG 5 cut(s) 257, 579, 941, 1076, 1125
HpySE526I ACGT 3 cut(s) 132, 691, 1131
Hsp92I GRCGYC 1 cut(s) 9
Hsp92II CATG 5 cut(s) 179, 671, 868, 877, 881
HspAI GCGC 1 cut(s) 9
KasI GGCGCC 1 cut(s) 8
Ksp22I TGATCA 1 cut(s) 967
KspAI GTTAAC 1 cut(s) 925
Kzo9I GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
LguI GCTCTTC 1 cut(s) 119
LmnI GCTCC 3 cut(s) 253, 625, 664
Lsp1109I GCAGC 4 cut(s) 4, 824, 929, 1186
LweI GCATC 3 cut(s) 87, 474, 532
MaeI CTAG 2 cut(s) 749, 861
MaeII ACGT 3 cut(s) 132, 691, 1131
MaeIII GTNAC 1 cut(s) 790
MalI GATC 6 cut(s) 27, 41, 654, 906, 969, 1048
MboI GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
MboII GAAGA 7 cut(s) 82, 136, 493, 594, 777, 957, 967
MflI RGATCY 1 cut(s) 39
MhlI GDGCHC 3 cut(s) 622, 641, 1084
MluCI AATT 8 cut(s) 345, 500, 610, 728, 763, 834, 1151, 1198
MluI ACGCGT 1 cut(s) 144
Mly113I GGCGCC 1 cut(s) 9
MlyI GAGTC 1 cut(s) 1007
MmeI TCCRAC 1 cut(s) 137
Mph1103I ATGCAT 1 cut(s) 879
MseI TTAA 3 cut(s) 264, 609, 924
MslI CAYNNNNRTG 1 cut(s) 411
MspA1I CMGCKG 1 cut(s) 361
MspI CCGG 5 cut(s) 12, 368, 402, 420, 425
MspR9I CCNGG 4 cut(s) 97, 369, 425, 489
Mva1269I GAATGC 1 cut(s) 194
MvaI CCWGG 2 cut(s) 97, 489
MvnI CGCG 3 cut(s) 30, 146, 559
MwoI GCNNNNNNNGC 2 cut(s) 17, 870
NarI GGCGCC 1 cut(s) 9
NciI CCSGG 2 cut(s) 369, 425
NdeII GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
NlaIII CATG 5 cut(s) 179, 671, 868, 877, 881
NlaIV GGNNCC 2 cut(s) 10, 739
NsiI ATGCAT 1 cut(s) 879
NspI RCATGY 1 cut(s) 877
PaeI GCATGC 1 cut(s) 877
PaeR7I CTCGAG 1 cut(s) 1234
PciSI GCTCTTC 1 cut(s) 119
PctI GAATGC 1 cut(s) 194
PfeI GAWTC 5 cut(s) 68, 106, 416, 566, 997
PkrI GCNGC 6 cut(s) 19, 32, 35, 814, 919, 1176
PleI GAGTC 1 cut(s) 1007
PluTI GGCGCC 1 cut(s) 12
PpsI GAGTC 1 cut(s) 1007
PsiI TTATAA 1 cut(s) 759
Psp1406I AACGTT 1 cut(s) 691
Psp6I CCWGG 2 cut(s) 95, 487
PspGI CCWGG 2 cut(s) 95, 487
PspN4I GGNNCC 2 cut(s) 10, 739
PspPI GGNCC 1 cut(s) 422
PstNI CAGNNNCTG 1 cut(s) 595
PsuI RGATCY 1 cut(s) 39
PvuII CAGCTG 1 cut(s) 361
RsaI GTAC 6 cut(s) 143, 304, 320, 451, 1009, 1092
RsaNI GTAC 6 cut(s) 142, 303, 319, 450, 1008, 1091
RseI CAYNNNNRTG 1 cut(s) 411
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 3 cut(s) 264, 609, 924
SatI GCNGC 6 cut(s) 18, 31, 34, 813, 918, 1175
Sau3AI GATC 6 cut(s) 25, 39, 652, 904, 967, 1046
Sau96I GGNCC 1 cut(s) 422
SchI GAGTC 1 cut(s) 1007
ScrFI CCNGG 4 cut(s) 97, 369, 425, 489
SduI GDGCHC 3 cut(s) 622, 641, 1084
SfaNI GCATC 3 cut(s) 87, 474, 532
SfoI GGCGCC 1 cut(s) 10
Sfr274I CTCGAG 1 cut(s) 1234
SgrAI CRCCGGYG 1 cut(s) 11
SlaI CTCGAG 1 cut(s) 1234
SmiMI CAYNNNNRTG 1 cut(s) 411
SmlI CTYRAG 1 cut(s) 1234
SmoI CTYRAG 1 cut(s) 1234
SphI GCATGC 1 cut(s) 877
Sse9I AATT 8 cut(s) 345, 500, 610, 728, 763, 834, 1151, 1198
SsiI CCGC 4 cut(s) 30, 33, 36, 990
SspDI GGCGCC 1 cut(s) 8
SspMI CTAG 2 cut(s) 749, 861
StyD4I CCNGG 4 cut(s) 95, 367, 423, 487
StyI CCWWGG 2 cut(s) 445, 748
TaaI ACNGT 1 cut(s) 1088
TaiI ACGT 3 cut(s) 135, 694, 1134
TaqI TCGA 3 cut(s) 52, 104, 1235
TasI AATT 8 cut(s) 345, 500, 610, 728, 763, 834, 1151, 1198
TatI WGTACW 2 cut(s) 318, 1090
TauI GCSGC 2 cut(s) 33, 36
TfiI GAWTC 5 cut(s) 68, 106, 416, 566, 997
Tru1I TTAA 3 cut(s) 264, 609, 924
Tru9I TTAA 3 cut(s) 264, 609, 924
TscAI CASTG 2 cut(s) 578, 1093
TseI GCWGC 4 cut(s) 17, 812, 917, 1174
TspDTI ATGAA 2 cut(s) 483, 1047
TspGWI ACGGA 1 cut(s) 1217
TspRI CASTG 2 cut(s) 578, 1093
VneI GTGCAC 1 cut(s) 1080
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 4 cut(s) 345, 834, 1151, 1198
XceI RCATGY 1 cut(s) 877
XhoI CTCGAG 1 cut(s) 1234
XmaJI CCTAGG 1 cut(s) 748
XspI CTAG 2 cut(s) 749, 861
Zsp2I ATGCAT 1 cut(s) 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.