pycom05g07120

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010502
Physical Location & Seq
Forward (+)
10105991 .. 10108806
2816 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07120.1

Sequence Viewer

Length: 1284 bp
ATGTGGAGCGGTGGCGAAGCTAGTGGTAGTGGCGGTGGTGCTGGCGTCGGCCGTGGAATCTACTGGGGACGGAAGGAGGAGGTCTCTGATTCCAAAGGAATCGTTGTTATCTTCGCTTGGGTTTCGATTCAGGAGAGGCACTTGCAGAATCACGTGGATTTATACTCGTCTCTCGGTTGGAATTCCCTCGTTTGCCATTCCCATTTTCTCCATGCATTCTACCCCGAAAAGGCCATGTCGTCCGCCTTTGTTATTCTCAACGCGCTTGTTGAGGAGCTAAGGGTTAAGCCATGTCCTGTAGTCTTTGTGGCTCTTTCTGCTGGTACAAAAGCTTGTATGTACAAGGTTTTTCGGATTATTGACGGAATTTGTGAAGGTCAGCTTTATCCATTATTTAACTTTGTGAAGGCTGAATATCAATTGGTCAGAAAGTGTATTACTGGACACATCTATGATTCTGGTCCAGTTGATTTTACAAGTGATCTGGGCACTCAATATGCTCTACACCCAACCATTCTAAAGATGCCTCGATCGTCAAAACTGGTTTCTTGGGTAGCTAAAGGCATTGCTTCCGGTTTGGATGCGTTGTATCTTACTAGATTTGAATCCCAGTGCGCTGAGTATTGGCACGCTCTGTTTTCGTCTGTTAATTTGGGTGCTCCATTTCTCGTTCTGTGCTCTGATAAAGATGATCTGGCCCCCTATCATATTATCTGCAATTTCACTCAACGTTTACAAGAACTTGGAGGAGATGTCAAGCTTGTGAAATTGAATGGCTCCCCTCACCTAGGTCATTATAAGCATTATCCAATACAGTATAGGTCTTCCGTTACTCATTTTCTTGAAAAGGCTGCCTCGGTATTTTCTCAAAGAATTAAAGAACTTGAAGGTGAAAATACTAGCATGGAAGGCATGCAAGATGAGATATCTGAACTAATATGTGATCTCCAGAAAGCAGCAGTTAACTCTAATCAGAGCCTTAGAAGAGTTGCAGTGGAGCCAAGCGACCACTTCTTCTTGCCAAGTTCAGCTGAGAATCAAAATAGTACAGGCTCTGGGTCTTTACAAGATGAACAAAAAGAAAGATCCGTCTCTCTGCCCAGTCCCCCAAGCATTAGTGCACATAGTGTACTCGGCCAAGTCCTGTTTGATGTTTGTGTCCCTAAGAACGTTGAGGGTTGGGATATTAAATTTTGCGGGTCTTTGAATGGGCAGCCATTTGCCTCTGCTCGTAGGAATTCACCTCCTCGTGGTCTCAAAAGCTTTCGTCGCTCAAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

428

Amino Acids

47.23

Weight (kDa)

7.6

Isoelectric Point (pI)

49.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 146 - 394 5.7e-27 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 798
AccBSI CCGCTC 1 cut(s) 9
AccII CGCG 1 cut(s) 263
AciI CCGC 4 cut(s) 9, 33, 243, 1197
AclI AACGTT 2 cut(s) 730, 1170
AclWI GGATC 1 cut(s) 1080
AcoI YGGCCR 2 cut(s) 49, 1135
AcsI RAATTY 4 cut(s) 181, 366, 1190, 1237
AcvI CACGTG 1 cut(s) 154
AcyI GRCGYC 1 cut(s) 45
AdeI CACNNNGTG 1 cut(s) 1127
AfaI GTAC 4 cut(s) 325, 341, 1048, 1131
AfiI CCNNNNNNNGG 3 cut(s) 229, 788, 1250
AgsI TTSAA 5 cut(s) 605, 772, 845, 887, 1207
AluBI AGCT 8 cut(s) 20, 277, 332, 382, 557, 760, 1031, 1263
AluI AGCT 8 cut(s) 20, 277, 332, 382, 557, 760, 1031, 1263
Alw21I GWGCWC 3 cut(s) 661, 680, 1123
Alw26I GTCTC 4 cut(s) 88, 174, 1096, 1259
Alw44I GTGCAC 1 cut(s) 1119
AlwI GGATC 1 cut(s) 1080
AlwNI CAGNNNCTG 1 cut(s) 1055
AoxI GGCC 4 cut(s) 49, 231, 696, 1135
ApaLI GTGCAC 1 cut(s) 1119
ApeKI GCWGC 3 cut(s) 851, 956, 1213
ApoI RAATTY 4 cut(s) 181, 366, 1190, 1237
AspA2I CCTAGG 1 cut(s) 787
AspLEI GCGC 2 cut(s) 265, 617
AspS9I GGNCC 2 cut(s) 461, 697
AsuHPI GGTGA 3 cut(s) 776, 902, 1233
AvaII GGWCC 1 cut(s) 461
AvrII CCTAGG 1 cut(s) 787
BaeGI GKGCMC 2 cut(s) 491, 1123
BauI CACGAG 1 cut(s) 1248
BbrPI CACGTG 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 816
Bbv12I GWGCWC 3 cut(s) 661, 680, 1123
BbvI GCAGC 3 cut(s) 838, 968, 1225
BceAI ACGGC 1 cut(s) 36
BcoDI GTCTC 4 cut(s) 88, 174, 1096, 1259
BfaI CTAG 4 cut(s) 21, 597, 788, 900
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 3 cut(s) 852, 957, 1214
BlnI CCTAGG 1 cut(s) 787
BlsI GCNGC 3 cut(s) 853, 958, 1215
Bme18I GGWCC 1 cut(s) 461
BmgT120I GGNCC 2 cut(s) 461, 697
BmiI GGNNCC 3 cut(s) 699, 778, 999
BmrI ACTGGG 3 cut(s) 73, 604, 1095
BmsI GCATC 2 cut(s) 513, 571
BmuI ACTGGG 3 cut(s) 73, 604, 1095
BpiI GAAGAC 1 cut(s) 816
BplI GAGNNNNNCTC 2 cut(s) 68, 100
BpmI CTGGAG 1 cut(s) 932
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 1258
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 604
BsaHI GRCGYC 1 cut(s) 45
BsaI GGTCTC 2 cut(s) 88, 1259
BsaJI CCNNGG 3 cut(s) 52, 787, 855
BsaWI WCCGGW 1 cut(s) 572
BsaXI ACNNNNNCTCC 2 cut(s) 738, 768
Bsc4I CCNNNNNNNGG 3 cut(s) 229, 788, 1250
Bse1I ACTGG 6 cut(s) 68, 445, 464, 546, 610, 1101
Bse3DI GCAATG 1 cut(s) 564
Bse8I GATNNNNATC 1 cut(s) 604
BseDI CCNNGG 3 cut(s) 52, 787, 855
BseGI GGATG 1 cut(s) 586
BseJI GATNNNNATC 1 cut(s) 604
BseLI CCNNNNNNNGG 3 cut(s) 229, 788, 1250
BseMI GCAATG 1 cut(s) 564
BseMII CTCAG 2 cut(s) 609, 1023
BseNI ACTGG 6 cut(s) 68, 445, 464, 546, 610, 1101
BseRI GAGGAG 4 cut(s) 92, 287, 762, 1236
BseSI GKGCMC 2 cut(s) 491, 1123
BseX3I CGGCCG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 838, 968, 1225
Bsh1236I CGCG 1 cut(s) 263
Bsh1285I CGRYCG 2 cut(s) 52, 533
BshFI GGCC 4 cut(s) 51, 233, 698, 1137
BsiEI CGRYCG 2 cut(s) 52, 533
BsiHKAI GWGCWC 3 cut(s) 661, 680, 1123
BsiSI CCGG 1 cut(s) 573
BslFI GGGAC 3 cut(s) 81, 1089, 1145
BslI CCNNNNNNNGG 3 cut(s) 229, 788, 1250
BsmAI GTCTC 4 cut(s) 88, 174, 1096, 1259
BsmBI CGTCTC 2 cut(s) 174, 1096
BsmFI GGGAC 3 cut(s) 81, 1089, 1145
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 4 cut(s) 51, 233, 698, 1137
Bso31I GGTCTC 2 cut(s) 88, 1259
Bsp1286I GDGCHC 4 cut(s) 491, 661, 680, 1123
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 5 cut(s) 481, 530, 691, 943, 1085
BspACI CCGC 4 cut(s) 9, 33, 243, 1197
BspANI GGCC 4 cut(s) 51, 233, 698, 1137
BspCNI CTCAG 2 cut(s) 610, 1024
BspFNI CGCG 1 cut(s) 263
BspLI GGNNCC 3 cut(s) 699, 778, 999
BspPI GGATC 1 cut(s) 1080
BspTNI GGTCTC 2 cut(s) 88, 1259
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 564
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 6 cut(s) 68, 445, 464, 546, 610, 1101
BssECI CCNNGG 3 cut(s) 52, 787, 855
BssMI GATC 5 cut(s) 481, 530, 691, 943, 1085
BssNI GRCGYC 1 cut(s) 45
BssSI CACGAG 1 cut(s) 1248
BssT1I CCWWGG 1 cut(s) 787
Bst2BI CACGAG 1 cut(s) 1248
Bst4CI ACNGT 1 cut(s) 816
Bst6I CTCTTC 1 cut(s) 979
BstACI GRCGYC 1 cut(s) 45
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 3 cut(s) 43, 630, 914
BstDEI CTNAG 5 cut(s) 278, 618, 980, 1032, 1164
BstDSI CCRYGG 1 cut(s) 52
BstENI CCTNNNNNAGG 1 cut(s) 786
BstF5I GGATG 1 cut(s) 586
BstFNI CGCG 1 cut(s) 263
BstHHI GCGC 2 cut(s) 265, 617
BstKTI GATC 5 cut(s) 484, 533, 694, 946, 1088
BstMAI GTCTC 4 cut(s) 88, 174, 1096, 1259
BstMBI GATC 5 cut(s) 481, 530, 691, 943, 1085
BstMCI CGRYCG 2 cut(s) 52, 533
BstMWI GCNNNNNNNGC 3 cut(s) 317, 909, 1269
BstNSI RCATGY 1 cut(s) 916
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 2 cut(s) 491, 1123
BstUI CGCG 1 cut(s) 263
BstV1I GCAGC 3 cut(s) 838, 968, 1225
BstV2I GAAGAC 1 cut(s) 816
BstX2I RGATCY 1 cut(s) 1085
BstYI RGATCY 1 cut(s) 1085
BstZI CGGCCG 1 cut(s) 49
BsuRI GGCC 4 cut(s) 51, 233, 698, 1137
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 2 cut(s) 617, 999
Cac8I GCNNGC 3 cut(s) 43, 630, 914
CaiI CAGNNNCTG 1 cut(s) 1055
CfoI GCGC 2 cut(s) 265, 617
Cfr13I GGNCC 2 cut(s) 461, 697
CseI GACGC 1 cut(s) 34
Csp6I GTAC 4 cut(s) 324, 340, 1047, 1130
CviAII CATG 5 cut(s) 212, 235, 291, 904, 913
CviQI GTAC 4 cut(s) 324, 340, 1047, 1130
DdeI CTNAG 5 cut(s) 278, 618, 980, 1032, 1164
DpnI GATC 5 cut(s) 483, 532, 693, 945, 1087
DpnII GATC 5 cut(s) 481, 530, 691, 943, 1085
DraIII CACNNNGTG 1 cut(s) 1127
EaeI YGGCCR 2 cut(s) 49, 1135
EagI CGGCCG 1 cut(s) 49
Eam1104I CTCTTC 1 cut(s) 979
EarI CTCTTC 1 cut(s) 979
EciI GGCGGA 1 cut(s) 232
EclXI CGGCCG 1 cut(s) 49
Eco130I CCWWGG 1 cut(s) 787
Eco31I GGTCTC 2 cut(s) 88, 1259
Eco32I GATATC 1 cut(s) 927
Eco47I GGWCC 1 cut(s) 461
Eco52I CGGCCG 1 cut(s) 49
Eco72I CACGTG 1 cut(s) 154
EcoNI CCTNNNNNAGG 1 cut(s) 786
EcoRI GAATTC 2 cut(s) 181, 1237
EcoRV GATATC 1 cut(s) 927
EcoT14I CCWWGG 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 217
ErhI CCWWGG 1 cut(s) 787
Esp3I CGTCTC 2 cut(s) 174, 1096
FaeI CATG 5 cut(s) 215, 238, 294, 907, 916
FalI AAGNNNNNCTT 2 cut(s) 366, 398
FaqI GGGAC 3 cut(s) 81, 1089, 1145
FatI CATG 5 cut(s) 211, 234, 290, 903, 912
FauI CCCGC 1 cut(s) 1190
Fnu4HI GCNGC 3 cut(s) 852, 957, 1214
FokI GGATG 1 cut(s) 593
Fsp4HI GCNGC 3 cut(s) 852, 957, 1214
FspBI CTAG 4 cut(s) 21, 597, 788, 900
GlaI GCGC 2 cut(s) 264, 616
GluI GCNGC 3 cut(s) 852, 957, 1214
GsuI CTGGAG 1 cut(s) 932
HaeIII GGCC 4 cut(s) 51, 233, 698, 1137
HapII CCGG 1 cut(s) 573
HgaI GACGC 1 cut(s) 34
HhaI GCGC 2 cut(s) 265, 617
Hin1I GRCGYC 1 cut(s) 45
Hin1II CATG 5 cut(s) 215, 238, 294, 907, 916
Hin6I GCGC 2 cut(s) 263, 615
HinP1I GCGC 2 cut(s) 263, 615
HincII GTYRAC 1 cut(s) 964
HindII GTYRAC 1 cut(s) 964
HindIII AAGCTT 3 cut(s) 330, 758, 1261
HinfI GANTC 8 cut(s) 57, 89, 99, 127, 148, 455, 605, 1036
HpaI GTTAAC 1 cut(s) 964
HpaII CCGG 1 cut(s) 573
HphI GGTGA 3 cut(s) 776, 902, 1233
Hpy166II GTNNAC 4 cut(s) 734, 964, 1121, 1130
Hpy188I TCNGA 6 cut(s) 88, 354, 428, 682, 931, 975
Hpy188III TCNNGA 4 cut(s) 131, 842, 949, 1275
Hpy8I GTNNAC 4 cut(s) 734, 964, 1121, 1130
Hpy99I CGWCG 2 cut(s) 50, 1272
HpyAV CCTTC 5 cut(s) 67, 368, 400, 881, 902
HpyCH4III ACNGT 1 cut(s) 816
HpyCH4IV ACGT 3 cut(s) 153, 730, 1170
HpyCH4V TGCA 6 cut(s) 145, 215, 717, 916, 992, 1121
HpyF10VI GCNNNNNNNGC 3 cut(s) 317, 909, 1269
HpyF3I CTNAG 5 cut(s) 278, 618, 980, 1032, 1164
HpySE526I ACGT 3 cut(s) 153, 730, 1170
Hsp92I GRCGYC 1 cut(s) 45
Hsp92II CATG 5 cut(s) 215, 238, 294, 907, 916
HspAI GCGC 2 cut(s) 263, 615
KspAI GTTAAC 1 cut(s) 964
Kzo9I GATC 5 cut(s) 481, 530, 691, 943, 1085
LmnI GCTCC 5 cut(s) 6, 274, 664, 782, 997
Lsp1109I GCAGC 3 cut(s) 838, 968, 1225
LweI GCATC 2 cut(s) 513, 571
MaeI CTAG 4 cut(s) 21, 597, 788, 900
MaeII ACGT 3 cut(s) 153, 730, 1170
MaeIII GTNAC 1 cut(s) 829
MalI GATC 5 cut(s) 483, 532, 693, 945, 1087
MbiI CCGCTC 1 cut(s) 9
MboI GATC 5 cut(s) 481, 530, 691, 943, 1085
MboII GAAGA 4 cut(s) 103, 816, 996, 1006
MfeI CAATTG 1 cut(s) 419
MflI RGATCY 1 cut(s) 1085
MhlI GDGCHC 4 cut(s) 491, 661, 680, 1123
MluCI AATT 9 cut(s) 181, 366, 419, 649, 718, 767, 873, 1190, 1237
MmeI TCCRAC 1 cut(s) 158
Mph1103I ATGCAT 1 cut(s) 217
MseI TTAA 6 cut(s) 285, 396, 648, 876, 963, 1188
MslI CAYNNNNRTG 1 cut(s) 450
MspA1I CMGCKG 1 cut(s) 1031
MspI CCGG 1 cut(s) 573
MunI CAATTG 1 cut(s) 419
Mva1269I GAATGC 1 cut(s) 215
MvnI CGCG 1 cut(s) 263
MwoI GCNNNNNNNGC 3 cut(s) 317, 909, 1269
NdeII GATC 5 cut(s) 481, 530, 691, 943, 1085
NlaIII CATG 5 cut(s) 215, 238, 294, 907, 916
NlaIV GGNNCC 3 cut(s) 699, 778, 999
NmeAIII GCCGAG 1 cut(s) 1113
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 1 cut(s) 916
PaeI GCATGC 1 cut(s) 916
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 8 cut(s) 57, 89, 99, 127, 148, 455, 605, 1036
PkrI GCNGC 3 cut(s) 853, 958, 1215
Ple19I CGATCG 1 cut(s) 533
PmaCI CACGTG 1 cut(s) 154
PmlI CACGTG 1 cut(s) 154
Ppu21I YACGTR 1 cut(s) 154
PsiI TTATAA 1 cut(s) 798
Psp1406I AACGTT 2 cut(s) 730, 1170
PspCI CACGTG 1 cut(s) 154
PspN4I GGNNCC 3 cut(s) 699, 778, 999
PspPI GGNCC 2 cut(s) 461, 697
PstNI CAGNNNCTG 1 cut(s) 1055
PsuI RGATCY 1 cut(s) 1085
PvuI CGATCG 1 cut(s) 533
PvuII CAGCTG 1 cut(s) 1031
RsaI GTAC 4 cut(s) 325, 341, 1048, 1131
RsaNI GTAC 4 cut(s) 324, 340, 1047, 1130
RseI CAYNNNNRTG 1 cut(s) 450
SaqAI TTAA 6 cut(s) 285, 396, 648, 876, 963, 1188
SatI GCNGC 3 cut(s) 852, 957, 1214
Sau3AI GATC 5 cut(s) 481, 530, 691, 943, 1085
Sau96I GGNCC 2 cut(s) 461, 697
SduI GDGCHC 4 cut(s) 491, 661, 680, 1123
SfaNI GCATC 2 cut(s) 513, 571
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 1 cut(s) 461
SmiMI CAYNNNNRTG 1 cut(s) 450
SmlI CTYRAG 1 cut(s) 1273
SmoI CTYRAG 1 cut(s) 1273
SphI GCATGC 1 cut(s) 916
Sse9I AATT 9 cut(s) 181, 366, 419, 649, 718, 767, 873, 1190, 1237
SsiI CCGC 4 cut(s) 9, 33, 243, 1197
SspMI CTAG 4 cut(s) 21, 597, 788, 900
StyI CCWWGG 1 cut(s) 787
TaaI ACNGT 1 cut(s) 816
TaiI ACGT 3 cut(s) 156, 733, 1173
TaqI TCGA 2 cut(s) 125, 529
TasI AATT 9 cut(s) 181, 366, 419, 649, 718, 767, 873, 1190, 1237
TatI WGTACW 3 cut(s) 339, 1046, 1129
TfiI GAWTC 8 cut(s) 57, 89, 99, 127, 148, 455, 605, 1036
Tru1I TTAA 6 cut(s) 285, 396, 648, 876, 963, 1188
Tru9I TTAA 6 cut(s) 285, 396, 648, 876, 963, 1188
TscAI CASTG 2 cut(s) 617, 999
TseI GCWGC 3 cut(s) 851, 956, 1213
TspDTI ATGAA 1 cut(s) 1086
TspGWI ACGGA 4 cut(s) 85, 378, 817, 1078
TspRI CASTG 2 cut(s) 617, 999
VneI GTGCAC 1 cut(s) 1119
VpaK11BI GGWCC 1 cut(s) 461
XagI CCTNNNNNAGG 1 cut(s) 786
XapI RAATTY 4 cut(s) 181, 366, 1190, 1237
XceI RCATGY 1 cut(s) 916
XmaJI CCTAGG 1 cut(s) 787
XspI CTAG 4 cut(s) 21, 597, 788, 900
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.