MD10G1092600.v1.1

Eukaryotic protein of unknown function (DUF829)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0010502
Physical Location & Seq
Forward (+)
14153677 .. 14157511
3835 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1092600.v1.1.491

Sequence Viewer

Length: 1266 bp
ATGTGGAGCGGTGGCGGAGCTAGTGGTAGTTGCGGTGGCGATGGTGGCGGCGGTGGAATCTACTGGGGACGGAAGGAGGAGGTCTCTCATTCCAAAGGAATCGTTGTCATCTTCGCTTGGGTTTCGATTCACGAGAGGCACTTGCAGAATCACGTGGATTTGTACTCGTCTCTCGGTTGGAATTCCCTCGTTTGCCATTCCCATTTTCTCCATGCATTCTATCCCGAAAAGGCCATGTCGTTCGCCTTTGTTATTCTCAATGAGCTTGTTGAGGAGCTAAGGGTTAAGCCATGTCCTGTAGTCTTTGTGGCTCTTTCTGCTGGTACAAAAGCTTGTATGTACAAGGTTTTTCGGATTCTTGACGGAATTTGTGACGGTCAGCATTATCTGGCTGAATATCAATTGGTCAGAAAGTGTATTACTGGACACATCTATGATTCTGGTCCTGTTGATTTTACAAGTGATCTGGGCACTCAATATGCTCTACACCCAACCATTCTAAAGATGCCTGGATCGTCAAAACTGGTTTCTTGGTTAGCTAAAGGCATTGCTTCTGGTTTGGATGCGTTGTACCTTACTAGATTTGAATCCCAATGTGCTGAGTATTGGCACGCTCTTTTTTCGTCTGTTAGTTTGGGTGCTCCATTTCTCATTTTGTGCTCTGATAAAGATGATCTGGCCCCTTATCACATTATCTGCAATTTCACTCACCGTTTACAAGAACTTGGAGGAGATGTCAAGCTTGTGAAATTGAATGGCTCCCCTCACCTAGATCATTATAAGTATTATCCAATACAGTATAGGTCTTCCGTTACTCACTTTCTTGAAAAGGCTGCTTTGGTTTTTTCTCAAAGAATTAAAGAACTTGAAGGTGACAATACTAGCATGGAAGGCATGCAAGATGAGATATCTGAACTAATATGTGATCTCCGGAAAGCAGCAGTTAACTCCAATCAGAGCCTTAGAAGAGTTGCAGTGGAGCCAAGCGACCACTTCTTCTTGCCAAGTTCAGCTGAGAATCAAAATAGTAAGGACTCCGGGTCTTTACAAGATGAACAAAAAGAAAGATCCGTCTCTCTGCCCAGTCCCCCAAGCATTAGTGCACATAGTGTACTTGGCCAAGTCCTGTTTGATGTTTGTGTCCCTAAGAACGTTGAGGGTTGGGATATTAAATTTTGCGGGTCTTTGAATGGGCAGCCATTTGCCTCTGCTCTTAGGAATTCACCTCCTCGTGGTCTCAAAAGCTTTCGTCGCTCAAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

422

Amino Acids

46.61

Weight (kDa)

6.65

Isoelectric Point (pI)

52.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 140 - 388 1.8e-28 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 780
AccBSI CCGCTC 1 cut(s) 9
AccIII TCCGGA 1 cut(s) 930
AciI CCGC 6 cut(s) 9, 15, 33, 48, 51, 1179
AclI AACGTT 1 cut(s) 1152
AclWI GGATC 2 cut(s) 520, 1062
AcoI YGGCCR 1 cut(s) 1117
AcsI RAATTY 4 cut(s) 181, 366, 1172, 1219
AcvI CACGTG 1 cut(s) 154
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 5 cut(s) 164, 325, 341, 572, 1113
AfiI CCNNNNNNNGG 1 cut(s) 1232
AgsI TTSAA 5 cut(s) 587, 754, 827, 869, 1189
AhdI GACNNNNNGTC 1 cut(s) 1039
AjnI CCWGG 1 cut(s) 508
AluBI AGCT 8 cut(s) 20, 265, 277, 332, 539, 742, 1013, 1245
AluI AGCT 8 cut(s) 20, 265, 277, 332, 539, 742, 1013, 1245
Alw21I GWGCWC 3 cut(s) 643, 662, 1105
Alw26I GTCTC 4 cut(s) 88, 174, 1078, 1241
Alw44I GTGCAC 1 cut(s) 1101
AlwI GGATC 2 cut(s) 520, 1062
Aor13HI TCCGGA 1 cut(s) 930
AoxI GGCC 3 cut(s) 231, 678, 1117
ApaLI GTGCAC 1 cut(s) 1101
ApeKI GCWGC 3 cut(s) 833, 938, 1195
ApoI RAATTY 4 cut(s) 181, 366, 1172, 1219
AspS9I GGNCC 2 cut(s) 443, 679
AsuC2I CCSGG 1 cut(s) 1039
AsuHPI GGTGA 4 cut(s) 701, 758, 884, 1215
AvaII GGWCC 1 cut(s) 443
BaeGI GKGCMC 2 cut(s) 473, 1105
BalI TGGCCA 1 cut(s) 1119
BauI CACGAG 2 cut(s) 131, 1230
BbrPI CACGTG 1 cut(s) 154
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 3 cut(s) 643, 662, 1105
BbvI GCAGC 3 cut(s) 820, 950, 1207
BccI CCATC 1 cut(s) 35
BciT130I CCWGG 1 cut(s) 510
BcnI CCSGG 1 cut(s) 1039
BcoDI GTCTC 4 cut(s) 88, 174, 1078, 1241
BfaI CTAG 4 cut(s) 21, 579, 770, 882
BfmI CTRYAG 1 cut(s) 297
BisI GCNGC 4 cut(s) 49, 834, 939, 1196
BlsI GCNGC 4 cut(s) 50, 835, 940, 1197
Bme1390I CCNGG 2 cut(s) 510, 1039
Bme18I GGWCC 1 cut(s) 443
BmeRI GACNNNNNGTC 1 cut(s) 1039
BmgT120I GGNCC 2 cut(s) 443, 679
BmiI GGNNCC 3 cut(s) 681, 760, 981
BmrFI CCNGG 2 cut(s) 510, 1039
BmrI ACTGGG 2 cut(s) 73, 1077
BmsI GCATC 2 cut(s) 495, 553
BmuI ACTGGG 2 cut(s) 73, 1077
BpiI GAAGAC 1 cut(s) 798
BplI GAGNNNNNCTC 2 cut(s) 68, 100
Bpu10I CCTNAGC 1 cut(s) 278
BpuEI CTTGAG 1 cut(s) 1240
BpuMI CCSGG 1 cut(s) 1039
BsaAI YACGTR 1 cut(s) 154
BsaBI GATNNNNATC 1 cut(s) 586
BsaI GGTCTC 2 cut(s) 88, 1241
BsaWI WCCGGW 1 cut(s) 930
BsaXI ACNNNNNCTCC 2 cut(s) 720, 750
Bsc4I CCNNNNNNNGG 1 cut(s) 1232
Bse1I ACTGG 4 cut(s) 68, 427, 528, 1083
Bse3DI GCAATG 1 cut(s) 546
Bse8I GATNNNNATC 1 cut(s) 586
BseAI TCCGGA 1 cut(s) 930
BseBI CCWGG 1 cut(s) 510
BseGI GGATG 1 cut(s) 568
BseJI GATNNNNATC 1 cut(s) 586
BseLI CCNNNNNNNGG 1 cut(s) 1232
BseMI GCAATG 1 cut(s) 546
BseMII CTCAG 2 cut(s) 591, 1005
BseNI ACTGG 4 cut(s) 68, 427, 528, 1083
BseRI GAGGAG 4 cut(s) 92, 287, 744, 1218
BseSI GKGCMC 2 cut(s) 473, 1105
BseXI GCAGC 3 cut(s) 820, 950, 1207
BshFI GGCC 3 cut(s) 233, 680, 1119
BsiHKAI GWGCWC 3 cut(s) 643, 662, 1105
BsiSI CCGG 2 cut(s) 931, 1038
BslFI GGGAC 3 cut(s) 81, 1071, 1127
BslI CCNNNNNNNGG 1 cut(s) 1232
BsmAI GTCTC 4 cut(s) 88, 174, 1078, 1241
BsmBI CGTCTC 2 cut(s) 174, 1078
BsmFI GGGAC 3 cut(s) 81, 1071, 1127
BsmI GAATGC 1 cut(s) 215
BsnI GGCC 3 cut(s) 233, 680, 1119
Bso31I GGTCTC 2 cut(s) 88, 1241
Bsp1286I GDGCHC 4 cut(s) 473, 643, 662, 1105
Bsp13I TCCGGA 1 cut(s) 930
Bsp1407I TGTACA 1 cut(s) 339
Bsp143I GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
BspACI CCGC 6 cut(s) 9, 15, 33, 48, 51, 1179
BspANI GGCC 3 cut(s) 233, 680, 1119
BspCNI CTCAG 2 cut(s) 592, 1006
BspEI TCCGGA 1 cut(s) 930
BspLI GGNNCC 3 cut(s) 681, 760, 981
BspPI GGATC 2 cut(s) 520, 1062
BspTNI GGTCTC 2 cut(s) 88, 1241
BsrBI CCGCTC 1 cut(s) 9
BsrDI GCAATG 1 cut(s) 546
BsrGI TGTACA 1 cut(s) 339
BsrI ACTGG 4 cut(s) 68, 427, 528, 1083
BssMI GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
BssSI CACGAG 2 cut(s) 131, 1230
Bst2BI CACGAG 2 cut(s) 131, 1230
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 3 cut(s) 377, 713, 798
Bst6I CTCTTC 1 cut(s) 961
BstAUI TGTACA 1 cut(s) 339
BstBAI YACGTR 1 cut(s) 154
BstC8I GCNNGC 2 cut(s) 612, 896
BstDEI CTNAG 6 cut(s) 278, 600, 962, 1014, 1146, 1214
BstF5I GGATG 1 cut(s) 568
BstKTI GATC 6 cut(s) 466, 515, 676, 775, 928, 1070
BstMAI GTCTC 4 cut(s) 88, 174, 1078, 1241
BstMBI GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
BstMWI GCNNNNNNNGC 4 cut(s) 45, 317, 891, 1251
BstNI CCWGG 1 cut(s) 510
BstNSI RCATGY 1 cut(s) 898
BstSCI CCNGG 2 cut(s) 508, 1037
BstSFI CTRYAG 1 cut(s) 297
BstSLI GKGCMC 2 cut(s) 473, 1105
BstV1I GCAGC 3 cut(s) 820, 950, 1207
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 1067
BstYI RGATCY 1 cut(s) 1067
BsuRI GGCC 3 cut(s) 233, 680, 1119
BtgZI GCGATG 1 cut(s) 54
BtsCI GGATG 1 cut(s) 568
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 1 cut(s) 981
Cac8I GCNNGC 2 cut(s) 612, 896
Cfr13I GGNCC 2 cut(s) 443, 679
Csp6I GTAC 5 cut(s) 163, 324, 340, 571, 1112
CviAII CATG 5 cut(s) 212, 235, 291, 886, 895
CviQI GTAC 5 cut(s) 163, 324, 340, 571, 1112
DdeI CTNAG 6 cut(s) 278, 600, 962, 1014, 1146, 1214
DpnI GATC 6 cut(s) 465, 514, 675, 774, 927, 1069
DpnII GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
DraIII CACNNNGTG 1 cut(s) 1109
DriI GACNNNNNGTC 1 cut(s) 1039
EaeI YGGCCR 1 cut(s) 1117
Eam1104I CTCTTC 1 cut(s) 961
Eam1105I GACNNNNNGTC 1 cut(s) 1039
EarI CTCTTC 1 cut(s) 961
EciI GGCGGA 1 cut(s) 30
Eco31I GGTCTC 2 cut(s) 88, 1241
Eco32I GATATC 1 cut(s) 909
Eco47I GGWCC 1 cut(s) 443
Eco72I CACGTG 1 cut(s) 154
EcoRI GAATTC 2 cut(s) 181, 1219
EcoRII CCWGG 1 cut(s) 508
EcoRV GATATC 1 cut(s) 909
EcoT22I ATGCAT 1 cut(s) 217
Esp3I CGTCTC 2 cut(s) 174, 1078
FaeI CATG 5 cut(s) 215, 238, 294, 889, 898
FaqI GGGAC 3 cut(s) 81, 1071, 1127
FatI CATG 5 cut(s) 211, 234, 290, 885, 894
FauI CCCGC 1 cut(s) 1172
Fnu4HI GCNGC 4 cut(s) 49, 834, 939, 1196
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 4 cut(s) 49, 834, 939, 1196
FspBI CTAG 4 cut(s) 21, 579, 770, 882
GluI GCNGC 4 cut(s) 49, 834, 939, 1196
HaeIII GGCC 3 cut(s) 233, 680, 1119
HapII CCGG 2 cut(s) 931, 1038
Hin1II CATG 5 cut(s) 215, 238, 294, 889, 898
HincII GTYRAC 1 cut(s) 946
HindII GTYRAC 1 cut(s) 946
HindIII AAGCTT 3 cut(s) 330, 740, 1243
HinfI GANTC 9 cut(s) 57, 99, 127, 148, 355, 437, 587, 1018, 1034
HpaI GTTAAC 1 cut(s) 946
HpaII CCGG 2 cut(s) 931, 1038
HphI GGTGA 4 cut(s) 701, 758, 884, 1215
Hpy166II GTNNAC 4 cut(s) 716, 946, 1103, 1112
Hpy188I TCNGA 5 cut(s) 354, 410, 664, 913, 957
Hpy188III TCNNGA 6 cut(s) 131, 224, 359, 824, 931, 1257
Hpy8I GTNNAC 4 cut(s) 716, 946, 1103, 1112
Hpy99I CGWCG 1 cut(s) 1254
HpyAV CCTTC 3 cut(s) 67, 863, 884
HpyCH4III ACNGT 3 cut(s) 377, 713, 798
HpyCH4IV ACGT 2 cut(s) 153, 1152
HpyCH4V TGCA 6 cut(s) 145, 215, 699, 898, 974, 1103
HpyF10VI GCNNNNNNNGC 4 cut(s) 45, 317, 891, 1251
HpyF3I CTNAG 6 cut(s) 278, 600, 962, 1014, 1146, 1214
HpySE526I ACGT 2 cut(s) 153, 1152
Hsp92II CATG 5 cut(s) 215, 238, 294, 889, 898
Kpn2I TCCGGA 1 cut(s) 930
KspAI GTTAAC 1 cut(s) 946
Kzo9I GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
LmnI GCTCC 6 cut(s) 6, 17, 274, 646, 764, 979
Lsp1109I GCAGC 3 cut(s) 820, 950, 1207
LweI GCATC 2 cut(s) 495, 553
MaeI CTAG 4 cut(s) 21, 579, 770, 882
MaeII ACGT 2 cut(s) 153, 1152
MaeIII GTNAC 3 cut(s) 371, 811, 872
MalI GATC 6 cut(s) 465, 514, 675, 774, 927, 1069
MbiI CCGCTC 1 cut(s) 9
MboI GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
MboII GAAGA 4 cut(s) 103, 798, 978, 988
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 1 cut(s) 1067
MhlI GDGCHC 4 cut(s) 473, 643, 662, 1105
MlsI TGGCCA 1 cut(s) 1119
MluCI AATT 8 cut(s) 181, 366, 401, 700, 749, 855, 1172, 1219
MluNI TGGCCA 1 cut(s) 1119
MlyI GAGTC 1 cut(s) 1028
MmeI TCCRAC 1 cut(s) 158
Mox20I TGGCCA 1 cut(s) 1119
Mph1103I ATGCAT 1 cut(s) 217
MroI TCCGGA 1 cut(s) 930
MscI TGGCCA 1 cut(s) 1119
MseI TTAA 4 cut(s) 285, 858, 945, 1170
MslI CAYNNNNRTG 1 cut(s) 432
Msp20I TGGCCA 1 cut(s) 1119
MspA1I CMGCKG 1 cut(s) 1013
MspI CCGG 2 cut(s) 931, 1038
MspR9I CCNGG 2 cut(s) 510, 1039
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 215
MvaI CCWGG 1 cut(s) 510
MwoI GCNNNNNNNGC 4 cut(s) 45, 317, 891, 1251
NciI CCSGG 1 cut(s) 1039
NdeII GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
NlaIII CATG 5 cut(s) 215, 238, 294, 889, 898
NlaIV GGNNCC 3 cut(s) 681, 760, 981
NmuCI GTSAC 2 cut(s) 371, 872
NsiI ATGCAT 1 cut(s) 217
NspI RCATGY 1 cut(s) 898
PaeI GCATGC 1 cut(s) 898
PctI GAATGC 1 cut(s) 215
PfeI GAWTC 8 cut(s) 57, 99, 127, 148, 355, 437, 587, 1018
PkrI GCNGC 4 cut(s) 50, 835, 940, 1197
PleI GAGTC 1 cut(s) 1028
PmaCI CACGTG 1 cut(s) 154
PmlI CACGTG 1 cut(s) 154
PpsI GAGTC 1 cut(s) 1028
Ppu21I YACGTR 1 cut(s) 154
PsiI TTATAA 1 cut(s) 780
Psp1406I AACGTT 1 cut(s) 1152
Psp6I CCWGG 1 cut(s) 508
PspCI CACGTG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 3 cut(s) 681, 760, 981
PspPI GGNCC 2 cut(s) 443, 679
PsuI RGATCY 1 cut(s) 1067
PvuII CAGCTG 1 cut(s) 1013
RsaI GTAC 5 cut(s) 164, 325, 341, 572, 1113
RsaNI GTAC 5 cut(s) 163, 324, 340, 571, 1112
RseI CAYNNNNRTG 1 cut(s) 432
SaqAI TTAA 4 cut(s) 285, 858, 945, 1170
SatI GCNGC 4 cut(s) 49, 834, 939, 1196
Sau3AI GATC 6 cut(s) 463, 512, 673, 772, 925, 1067
Sau96I GGNCC 2 cut(s) 443, 679
SchI GAGTC 1 cut(s) 1028
ScrFI CCNGG 2 cut(s) 510, 1039
SduI GDGCHC 4 cut(s) 473, 643, 662, 1105
SfaNI GCATC 2 cut(s) 495, 553
SfcI CTRYAG 1 cut(s) 297
SinI GGWCC 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 432
SmlI CTYRAG 1 cut(s) 1255
SmoI CTYRAG 1 cut(s) 1255
SphI GCATGC 1 cut(s) 898
Sse9I AATT 8 cut(s) 181, 366, 401, 700, 749, 855, 1172, 1219
SsiI CCGC 6 cut(s) 9, 15, 33, 48, 51, 1179
SspMI CTAG 4 cut(s) 21, 579, 770, 882
StyD4I CCNGG 2 cut(s) 508, 1037
TaaI ACNGT 3 cut(s) 377, 713, 798
TaiI ACGT 2 cut(s) 156, 1155
TaqI TCGA 1 cut(s) 125
TasI AATT 8 cut(s) 181, 366, 401, 700, 749, 855, 1172, 1219
TatI WGTACW 3 cut(s) 162, 339, 1111
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 8 cut(s) 57, 99, 127, 148, 355, 437, 587, 1018
Tru1I TTAA 4 cut(s) 285, 858, 945, 1170
Tru9I TTAA 4 cut(s) 285, 858, 945, 1170
TscAI CASTG 1 cut(s) 981
TseFI GTSAC 2 cut(s) 371, 872
TseI GCWGC 3 cut(s) 833, 938, 1195
Tsp45I GTSAC 2 cut(s) 371, 872
TspDTI ATGAA 1 cut(s) 1068
TspGWI ACGGA 4 cut(s) 85, 378, 799, 1060
TspRI CASTG 1 cut(s) 981
VneI GTGCAC 1 cut(s) 1101
VpaK11BI GGWCC 1 cut(s) 443
XapI RAATTY 4 cut(s) 181, 366, 1172, 1219
XceI RCATGY 1 cut(s) 898
XspI CTAG 4 cut(s) 21, 579, 770, 882
Zsp2I ATGCAT 1 cut(s) 217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.