MD05G1093600.v1.1

Thioredoxin domain-containing protein 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0005041
Physical Location & Seq
Reverse (-)
19839014 .. 19841142
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1093600.v1.1.491

Sequence Viewer

Length: 630 bp
ATGGAGAACCGGGCGGTTCAAGAGATATTGGAGAAGCAATTGCTGACGGTTGCGAAGGCGGTGGAGGACAAGCTCGACGAGGAGATCGCAGCCCTAGATCGAGTTGACGATGACGATCTAGAGGCGCTTAGAGAGCGGAGGCTTCAGCAAATGAAGAAAATGGCGGAGAAGCGCAGCCGTTGGATCTCCCTCGGACATGGCGAGTACTCTGAGATTCCTTCCGAGAAGGACTTCTTCTCTGCCGTCAAGGCCAGCGACCGGGTCGTCTGCCACTTTTACCGCGAAAATTGGCCCTGCAAGGTAGTGGATAAGCACTTGAGTATATTGGCGAAGCAACACATGGAGACACGTTTTGTGAGGATCAATGCTGAGAAAAGTCCCTTCTTAGCGGAGAAGCTCAAGATTGTTGTTCTTCCAACCATTGCTCTTATTAAGAATGCCAAAGTGGATGATTATGTGGTTGGATTCGATGAGCTTGGTGGGAAAGACGACTTCAGCACAGAAGAACTGGAAGAGAGATTGGCTAAAGCCCAAGTCATTTTTTATGAAGGTGAATCGTCTATGAAGTCTAGTGCACAAACCAGGAGCGTCCGGCAAAGCAGCAAGGCCGACTCATCAGATTCAGAGTAA

Protein Analysis

210

Amino Acids

23.94

Weight (kDa)

5.34

Isoelectric Point (pI)

51.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 74 - 155 1.4e-07 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AccBSI CCGCTC 1 cut(s) 136
AccII CGCG 1 cut(s) 282
AciI CCGC 6 cut(s) 14, 59, 136, 164, 280, 389
AclWI GGATC 2 cut(s) 191, 368
AcuI CTGAAG 2 cut(s) 128, 478
AfaI GTAC 1 cut(s) 206
AfiI CCNNNNNNNGG 1 cut(s) 258
AflIII ACRYGT 1 cut(s) 347
AgsI TTSAA 1 cut(s) 20
AjnI CCWGG 1 cut(s) 581
AluBI AGCT 3 cut(s) 73, 397, 475
AluI AGCT 3 cut(s) 73, 397, 475
Alw21I GWGCWC 1 cut(s) 577
Alw26I GTCTC 1 cut(s) 338
Alw44I GTGCAC 1 cut(s) 573
AlwI GGATC 2 cut(s) 191, 368
AoxI GGCC 3 cut(s) 249, 290, 606
ApaLI GTGCAC 1 cut(s) 573
ApeKI GCWGC 3 cut(s) 89, 174, 600
Asp700I GAANNNNTTC 1 cut(s) 230
AspLEI GCGC 2 cut(s) 127, 174
AspS9I GGNCC 1 cut(s) 291
AsuC2I CCSGG 2 cut(s) 11, 260
AsuHPI GGTGA 1 cut(s) 563
BaeGI GKGCMC 1 cut(s) 577
Bbv12I GWGCWC 1 cut(s) 577
BbvI GCAGC 3 cut(s) 101, 186, 612
BceAI ACGGC 2 cut(s) 162, 227
BciT130I CCWGG 1 cut(s) 583
BcnI CCSGG 2 cut(s) 11, 260
BcoDI GTCTC 1 cut(s) 338
BfaI CTAG 3 cut(s) 95, 119, 570
BfoI RGCGCY 1 cut(s) 128
BglI GCCNNNNNGGC 1 cut(s) 248
BisI GCNGC 3 cut(s) 90, 175, 601
BlsI GCNGC 3 cut(s) 91, 176, 602
BmcAI AGTACT 1 cut(s) 206
Bme1390I CCNGG 3 cut(s) 11, 260, 583
BmgT120I GGNCC 1 cut(s) 291
BmrFI CCNGG 3 cut(s) 11, 260, 583
BpuEI CTTGAG 2 cut(s) 337, 383
BpuMI CCSGG 2 cut(s) 11, 260
BsaBI GATNNNNATC 1 cut(s) 114
BsaJI CCNNGG 1 cut(s) 190
Bsc4I CCNNNNNNNGG 1 cut(s) 258
Bse1I ACTGG 1 cut(s) 513
Bse3DI GCAATG 1 cut(s) 420
Bse8I GATNNNNATC 1 cut(s) 114
BseBI CCWGG 1 cut(s) 583
BseDI CCNNGG 1 cut(s) 190
BseGI GGATG 1 cut(s) 454
BseJI GATNNNNATC 1 cut(s) 114
BseLI CCNNNNNNNGG 1 cut(s) 258
BseMI GCAATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 201, 360
BseNI ACTGG 1 cut(s) 513
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 577
BseXI GCAGC 3 cut(s) 101, 186, 612
Bsh1236I CGCG 1 cut(s) 282
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 3 cut(s) 251, 292, 608
BsiEI CGRYCG 1 cut(s) 259
BsiHKAI GWGCWC 1 cut(s) 577
BsiSI CCGG 3 cut(s) 10, 259, 592
BslFI GGGAC 1 cut(s) 363
BslI CCNNNNNNNGG 1 cut(s) 258
BsmAI GTCTC 1 cut(s) 338
BsmFI GGGAC 1 cut(s) 363
BsmI GAATGC 1 cut(s) 442
BsnI GGCC 3 cut(s) 251, 292, 608
Bsp1286I GDGCHC 1 cut(s) 577
Bsp143I GATC 5 cut(s) 84, 97, 115, 183, 360
BspACI CCGC 6 cut(s) 14, 59, 136, 164, 280, 389
BspANI GGCC 3 cut(s) 251, 292, 608
BspCNI CTCAG 2 cut(s) 202, 361
BspFNI CGCG 1 cut(s) 282
BspPI GGATC 2 cut(s) 191, 368
BsrBI CCGCTC 1 cut(s) 136
BsrDI GCAATG 1 cut(s) 420
BsrI ACTGG 1 cut(s) 513
BssECI CCNNGG 1 cut(s) 190
BssMI GATC 5 cut(s) 84, 97, 115, 183, 360
Bst2UI CCWGG 1 cut(s) 583
Bst4CI ACNGT 1 cut(s) 49
Bst6I CTCTTC 1 cut(s) 507
BstC8I GCNNGC 1 cut(s) 253
BstDEI CTNAG 4 cut(s) 128, 210, 369, 385
BstF5I GGATG 1 cut(s) 454
BstFNI CGCG 1 cut(s) 282
BstH2I RGCGCY 1 cut(s) 128
BstHHI GCGC 2 cut(s) 127, 174
BstKTI GATC 5 cut(s) 87, 100, 118, 186, 363
BstMAI GTCTC 1 cut(s) 338
BstMBI GATC 5 cut(s) 84, 97, 115, 183, 360
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 2 cut(s) 133, 248
BstNI CCWGG 1 cut(s) 583
BstSCI CCNGG 3 cut(s) 9, 258, 581
BstSLI GKGCMC 1 cut(s) 577
BstUI CGCG 1 cut(s) 282
BstV1I GCAGC 3 cut(s) 101, 186, 612
BstX2I RGATCY 1 cut(s) 183
BstYI RGATCY 1 cut(s) 183
BsuRI GGCC 3 cut(s) 251, 292, 608
BtsCI GGATG 1 cut(s) 454
Cac8I GCNNGC 1 cut(s) 253
CfoI GCGC 2 cut(s) 127, 174
Cfr13I GGNCC 1 cut(s) 291
CseI GACGC 1 cut(s) 577
Csp6I GTAC 1 cut(s) 205
CviAII CATG 2 cut(s) 197, 340
CviQI GTAC 1 cut(s) 205
DdeI CTNAG 4 cut(s) 128, 210, 369, 385
DpnI GATC 5 cut(s) 86, 99, 117, 185, 362
DpnII GATC 5 cut(s) 84, 97, 115, 183, 360
DrdI GACNNNNNNGTC 1 cut(s) 263
DseDI GACNNNNNNGTC 1 cut(s) 263
Eam1104I CTCTTC 1 cut(s) 507
EarI CTCTTC 1 cut(s) 507
EciI GGCGGA 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 128, 478
EcoRII CCWGG 1 cut(s) 581
FaeI CATG 2 cut(s) 200, 343
FaiI YATR 6 cut(s) 198, 323, 341, 456, 546, 563
FalI AAGNNNNNCTT 2 cut(s) 218, 250
FaqI GGGAC 1 cut(s) 363
FatI CATG 2 cut(s) 196, 339
Fnu4HI GCNGC 3 cut(s) 90, 175, 601
FokI GGATG 1 cut(s) 461
Fsp4HI GCNGC 3 cut(s) 90, 175, 601
FspBI CTAG 3 cut(s) 95, 119, 570
GlaI GCGC 2 cut(s) 126, 173
GluI GCNGC 3 cut(s) 90, 175, 601
HaeII RGCGCY 1 cut(s) 128
HaeIII GGCC 3 cut(s) 251, 292, 608
HapII CCGG 3 cut(s) 10, 259, 592
HgaI GACGC 1 cut(s) 577
HhaI GCGC 2 cut(s) 127, 174
Hin1II CATG 2 cut(s) 200, 343
Hin6I GCGC 2 cut(s) 125, 172
HinP1I GCGC 2 cut(s) 125, 172
HincII GTYRAC 1 cut(s) 106
HindII GTYRAC 1 cut(s) 106
HinfI GANTC 5 cut(s) 214, 465, 554, 611, 620
HpaII CCGG 3 cut(s) 10, 259, 592
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 2 cut(s) 106, 575
Hpy188I TCNGA 5 cut(s) 194, 211, 223, 619, 625
Hpy188III TCNNGA 3 cut(s) 20, 119, 400
Hpy8I GTNNAC 2 cut(s) 106, 575
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 5 cut(s) 49, 220, 228, 391, 542
HpyCH4III ACNGT 1 cut(s) 49
HpyCH4IV ACGT 1 cut(s) 349
HpyCH4V TGCA 2 cut(s) 297, 575
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 248
HpyF3I CTNAG 4 cut(s) 128, 210, 369, 385
HpySE526I ACGT 1 cut(s) 349
Hsp92II CATG 2 cut(s) 200, 343
HspAI GCGC 2 cut(s) 125, 172
Kzo9I GATC 5 cut(s) 84, 97, 115, 183, 360
LmnI GCTCC 1 cut(s) 585
LpnPI CCDG 8 cut(s) 23, 265, 272, 307, 494, 568, 595, 605
Lsp1109I GCAGC 3 cut(s) 101, 186, 612
MaeI CTAG 3 cut(s) 95, 119, 570
MaeII ACGT 1 cut(s) 349
MalI GATC 5 cut(s) 86, 99, 117, 185, 362
MbiI CCGCTC 1 cut(s) 136
MboI GATC 5 cut(s) 84, 97, 115, 183, 360
MboII GAAGA 5 cut(s) 166, 226, 404, 515, 524
MfeI CAATTG 1 cut(s) 38
MflI RGATCY 1 cut(s) 183
MhlI GDGCHC 1 cut(s) 577
MluCI AATT 2 cut(s) 38, 286
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 3 cut(s) 161, 440, 442
MnlI CCTC 6 cut(s) 58, 73, 115, 132, 200, 351
MroXI GAANNNNTTC 1 cut(s) 230
MseI TTAA 1 cut(s) 432
MspI CCGG 3 cut(s) 10, 259, 592
MspR9I CCNGG 3 cut(s) 11, 260, 583
MunI CAATTG 1 cut(s) 38
Mva1269I GAATGC 1 cut(s) 442
MvaI CCWGG 1 cut(s) 583
MvnI CGCG 1 cut(s) 282
MwoI GCNNNNNNNGC 2 cut(s) 133, 248
NciI CCSGG 2 cut(s) 11, 260
NdeII GATC 5 cut(s) 84, 97, 115, 183, 360
NlaIII CATG 2 cut(s) 200, 343
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 230
PfeI GAWTC 4 cut(s) 214, 465, 554, 620
PflFI GACNNNGTC 1 cut(s) 260
PkrI GCNGC 3 cut(s) 91, 176, 602
PleI GAGTC 1 cut(s) 605
PpsI GAGTC 1 cut(s) 605
Psp6I CCWGG 1 cut(s) 581
PspGI CCWGG 1 cut(s) 581
PspPI GGNCC 1 cut(s) 291
PsuI RGATCY 1 cut(s) 183
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 1 cut(s) 206
RsaNI GTAC 1 cut(s) 205
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 3 cut(s) 90, 175, 601
Sau3AI GATC 5 cut(s) 84, 97, 115, 183, 360
Sau96I GGNCC 1 cut(s) 291
ScaI AGTACT 1 cut(s) 206
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 3 cut(s) 11, 260, 583
SduI GDGCHC 1 cut(s) 577
SetI ASST 6 cut(s) 75, 303, 352, 399, 477, 553
SmlI CTYRAG 2 cut(s) 316, 398
SmoI CTYRAG 2 cut(s) 316, 398
Sse9I AATT 2 cut(s) 38, 286
SsiI CCGC 6 cut(s) 14, 59, 136, 164, 280, 389
SspMI CTAG 3 cut(s) 95, 119, 570
StyD4I CCNGG 3 cut(s) 9, 258, 581
TaaI ACNGT 1 cut(s) 49
TaiI ACGT 1 cut(s) 352
TaqI TCGA 3 cut(s) 75, 100, 468
TasI AATT 2 cut(s) 38, 286
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 4 cut(s) 214, 465, 554, 620
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseI GCWGC 3 cut(s) 89, 174, 600
TspDTI ATGAA 3 cut(s) 167, 561, 578
Tth111I GACNNNGTC 1 cut(s) 260
VneI GTGCAC 1 cut(s) 573
XbaI TCTAGA 1 cut(s) 118
XmnI GAANNNNTTC 1 cut(s) 230
XspI CTAG 3 cut(s) 95, 119, 570
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.