Rh6BG061500

Thioredoxin domain-containing protein 9 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0005041
Physical Location & Seq
Forward (+)
9698935 .. 9700804
1870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG061500.1

Sequence Viewer

Length: 480 bp
ATGAAGAAGATGGCCGAGAAGCGCAGCCGTTGGATCTCCCTTGGCCACGGCGAGTACACCGAGATCCCTTCCGAGAAGGACTTCTTCTCCGCCGTCAAGGCCAGTGACCGCGTCGTCTGCCACTTTTACCGCGAAAATTGGCCCTGCAAGGTTGTGGACAAGCACTTGAGCATATTGGCAAAGCAACACATAGAGACACGTTTTATGAAGATCAATGCTGAGAAAAGTCCATTCTTAGCGGAGAAGCTCAAGATTGTTGTTCTTCCAACCCTTGCCCTTATTAAGAATGCCAAAGTGGATGATTATGTGGTTGGATTTGATGAGCTGGGTGGGAAAGACGACTTCAGCACAGAAGAACTAGAGGAGAGGTTGGCTAGAGCTCAAGTCATATTTTTTGAAGGTGAAGCATCTTTAAAGTCCAGTGCGAAAACCAGGAGTGTCCGGCAAAGCAGCAAGGATGACTCATCAGATTCAGAGTAA

Protein Analysis

159

Amino Acids

18.15

Weight (kDa)

6.92

Isoelectric Point (pI)

50.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Thioredoxin PF00085 24 - 105 3e-08 Thioredoxin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 113
AccII CGCG 2 cut(s) 111, 132
AciI CCGC 4 cut(s) 90, 109, 130, 239
AclWI GGATC 2 cut(s) 41, 58
AcoI YGGCCR 2 cut(s) 12, 43
AcuI CTGAAG 1 cut(s) 328
AfaI GTAC 1 cut(s) 56
AflIII ACRYGT 1 cut(s) 197
AgsI TTSAA 1 cut(s) 398
AjnI CCWGG 1 cut(s) 431
AluBI AGCT 3 cut(s) 247, 325, 380
AluI AGCT 3 cut(s) 247, 325, 380
Alw21I GWGCWC 1 cut(s) 382
Alw26I GTCTC 1 cut(s) 188
AlwI GGATC 2 cut(s) 41, 58
AoxI GGCC 4 cut(s) 12, 43, 99, 140
ApeKI GCWGC 2 cut(s) 24, 450
Asp700I GAANNNNTTC 1 cut(s) 80
AspLEI GCGC 1 cut(s) 24
AspS9I GGNCC 1 cut(s) 141
AsuHPI GGTGA 1 cut(s) 413
BalI TGGCCA 1 cut(s) 45
BanII GRGCYC 1 cut(s) 382
Bbv12I GWGCWC 1 cut(s) 382
BbvI GCAGC 2 cut(s) 36, 462
BccI CCATC 1 cut(s) 4
BceAI ACGGC 3 cut(s) 12, 64, 77
BciT130I CCWGG 1 cut(s) 433
BcoDI GTCTC 1 cut(s) 188
BfaI CTAG 2 cut(s) 359, 375
BglI GCCNNNNNGGC 1 cut(s) 98
BisI GCNGC 2 cut(s) 25, 451
BlsI GCNGC 2 cut(s) 26, 452
Bme1390I CCNGG 1 cut(s) 433
BmgT120I GGNCC 1 cut(s) 141
BmrFI CCNGG 1 cut(s) 433
BmsI GCATC 1 cut(s) 416
BpuEI CTTGAG 3 cut(s) 187, 233, 366
BsaJI CCNNGG 2 cut(s) 40, 46
BsaXI ACNNNNNCTCC 2 cut(s) 71, 101
Bse1I ACTGG 2 cut(s) 102, 420
BseBI CCWGG 1 cut(s) 433
BseDI CCNNGG 2 cut(s) 40, 46
BseGI GGATG 2 cut(s) 304, 463
BseMII CTCAG 1 cut(s) 210
BseNI ACTGG 2 cut(s) 102, 420
BseRI GAGGAG 1 cut(s) 377
BseXI GCAGC 2 cut(s) 36, 462
BseYI CCCAGC 1 cut(s) 325
Bsh1236I CGCG 2 cut(s) 111, 132
BshFI GGCC 4 cut(s) 14, 45, 101, 142
BsiHKAI GWGCWC 1 cut(s) 382
BsiSI CCGG 1 cut(s) 442
BsmAI GTCTC 1 cut(s) 188
BsmI GAATGC 1 cut(s) 292
BsnI GGCC 4 cut(s) 14, 45, 101, 142
Bsp1286I GDGCHC 1 cut(s) 382
Bsp143I GATC 3 cut(s) 33, 63, 210
BspACI CCGC 4 cut(s) 90, 109, 130, 239
BspANI GGCC 4 cut(s) 14, 45, 101, 142
BspCNI CTCAG 1 cut(s) 211
BspFNI CGCG 2 cut(s) 111, 132
BspPI GGATC 2 cut(s) 41, 58
BsrI ACTGG 2 cut(s) 102, 420
BssECI CCNNGG 2 cut(s) 40, 46
BssMI GATC 3 cut(s) 33, 63, 210
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 433
BstDEI CTNAG 2 cut(s) 219, 235
BstDSI CCRYGG 1 cut(s) 46
BstF5I GGATG 2 cut(s) 304, 463
BstFNI CGCG 2 cut(s) 111, 132
BstHHI GCGC 1 cut(s) 24
BstKTI GATC 3 cut(s) 36, 66, 213
BstMAI GTCTC 1 cut(s) 188
BstMBI GATC 3 cut(s) 33, 63, 210
BstMWI GCNNNNNNNGC 2 cut(s) 98, 117
BstNI CCWGG 1 cut(s) 433
BstSCI CCNGG 1 cut(s) 431
BstUI CGCG 2 cut(s) 111, 132
BstV1I GCAGC 2 cut(s) 36, 462
BstX2I RGATCY 2 cut(s) 33, 63
BstYI RGATCY 2 cut(s) 33, 63
BsuRI GGCC 4 cut(s) 14, 45, 101, 142
BtgI CCRYGG 1 cut(s) 46
BtsCI GGATG 2 cut(s) 304, 463
BtsIMutI CAGTG 2 cut(s) 109, 427
CfoI GCGC 1 cut(s) 24
Cfr13I GGNCC 1 cut(s) 141
CseI GACGC 1 cut(s) 100
Csp6I GTAC 1 cut(s) 55
CviJI RGCY 9 cut(s) 14, 27, 45, 101, 142, 247, 325, 374, 380
CviKI_1 RGCY 9 cut(s) 14, 27, 45, 101, 142, 247, 325, 374, 380
CviQI GTAC 1 cut(s) 55
DdeI CTNAG 2 cut(s) 219, 235
DpnI GATC 3 cut(s) 35, 65, 212
DpnII GATC 3 cut(s) 33, 63, 210
DraI TTTAAA 1 cut(s) 414
DrdI GACNNNNNNGTC 1 cut(s) 113
DseDI GACNNNNNNGTC 1 cut(s) 113
EaeI YGGCCR 2 cut(s) 12, 43
EciI GGCGGA 1 cut(s) 79
Ecl136II GAGCTC 1 cut(s) 380
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 1 cut(s) 382
Eco53kI GAGCTC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 328
EcoICRI GAGCTC 1 cut(s) 380
EcoRII CCWGG 1 cut(s) 431
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 40
FaiI YATR 5 cut(s) 173, 191, 206, 306, 389
FalI AAGNNNNNCTT 2 cut(s) 68, 100
Fnu4HI GCNGC 2 cut(s) 25, 451
FokI GGATG 2 cut(s) 311, 470
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 2 cut(s) 25, 451
FspBI CTAG 2 cut(s) 359, 375
GlaI GCGC 1 cut(s) 23
GluI GCNGC 2 cut(s) 25, 451
GsaI CCCAGC 1 cut(s) 329
HaeIII GGCC 4 cut(s) 14, 45, 101, 142
HapII CCGG 1 cut(s) 442
HgaI GACGC 1 cut(s) 100
HhaI GCGC 1 cut(s) 24
Hin6I GCGC 1 cut(s) 22
HinP1I GCGC 1 cut(s) 22
HinfI GANTC 2 cut(s) 461, 470
HpaII CCGG 1 cut(s) 442
HphI GGTGA 1 cut(s) 413
Hpy166II GTNNAC 2 cut(s) 57, 157
Hpy188I TCNGA 3 cut(s) 73, 469, 475
Hpy188III TCNNGA 1 cut(s) 250
Hpy8I GTNNAC 2 cut(s) 57, 157
Hpy99I CGWCG 1 cut(s) 116
HpyAV CCTTC 3 cut(s) 70, 78, 392
HpyCH4IV ACGT 1 cut(s) 199
HpyCH4V TGCA 1 cut(s) 147
HpyF10VI GCNNNNNNNGC 2 cut(s) 98, 117
HpyF3I CTNAG 2 cut(s) 219, 235
HpySE526I ACGT 1 cut(s) 199
HspAI GCGC 1 cut(s) 22
Kzo9I GATC 3 cut(s) 33, 63, 210
LpnPI CCDG 7 cut(s) 115, 157, 311, 418, 433, 445, 455
Lsp1109I GCAGC 2 cut(s) 36, 462
LweI GCATC 1 cut(s) 416
MaeI CTAG 2 cut(s) 359, 375
MaeII ACGT 1 cut(s) 199
MaeIII GTNAC 1 cut(s) 104
MalI GATC 3 cut(s) 35, 65, 212
MboI GATC 3 cut(s) 33, 63, 210
MboII GAAGA 6 cut(s) 16, 19, 76, 220, 254, 365
MflI RGATCY 2 cut(s) 33, 63
MhlI GDGCHC 1 cut(s) 382
MlsI TGGCCA 1 cut(s) 45
MluCI AATT 1 cut(s) 136
MluNI TGGCCA 1 cut(s) 45
MlyI GAGTC 1 cut(s) 455
MmeI TCCRAC 3 cut(s) 11, 290, 292
MnlI CCTC 2 cut(s) 355, 360
Mox20I TGGCCA 1 cut(s) 45
MroXI GAANNNNTTC 1 cut(s) 80
MscI TGGCCA 1 cut(s) 45
MseI TTAA 2 cut(s) 282, 413
Msp20I TGGCCA 1 cut(s) 45
MspI CCGG 1 cut(s) 442
MspR9I CCNGG 1 cut(s) 433
Mva1269I GAATGC 1 cut(s) 292
MvaI CCWGG 1 cut(s) 433
MvnI CGCG 2 cut(s) 111, 132
MwoI GCNNNNNNNGC 2 cut(s) 98, 117
NdeII GATC 3 cut(s) 33, 63, 210
NmeAIII GCCGAG 1 cut(s) 40
NmuCI GTSAC 1 cut(s) 104
PctI GAATGC 1 cut(s) 292
PdmI GAANNNNTTC 1 cut(s) 80
PfeI GAWTC 1 cut(s) 470
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 2 cut(s) 26, 452
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 382
Psp6I CCWGG 1 cut(s) 431
PspFI CCCAGC 1 cut(s) 325
PspGI CCWGG 1 cut(s) 431
PspPI GGNCC 1 cut(s) 141
PsuI RGATCY 2 cut(s) 33, 63
PsyI GACNNNGTC 1 cut(s) 110
RsaI GTAC 1 cut(s) 56
RsaNI GTAC 1 cut(s) 55
SacI GAGCTC 1 cut(s) 382
SaqAI TTAA 2 cut(s) 282, 413
SatI GCNGC 2 cut(s) 25, 451
Sau3AI GATC 3 cut(s) 33, 63, 210
Sau96I GGNCC 1 cut(s) 141
SchI GAGTC 1 cut(s) 455
ScrFI CCNGG 1 cut(s) 433
SduI GDGCHC 1 cut(s) 382
SetI ASST 7 cut(s) 153, 202, 249, 327, 371, 382, 403
SfaNI GCATC 1 cut(s) 416
SmlI CTYRAG 3 cut(s) 166, 248, 381
SmoI CTYRAG 3 cut(s) 166, 248, 381
Sse9I AATT 1 cut(s) 136
SsiI CCGC 4 cut(s) 90, 109, 130, 239
SspMI CTAG 2 cut(s) 359, 375
SstI GAGCTC 1 cut(s) 382
StyD4I CCNGG 1 cut(s) 431
StyI CCWWGG 1 cut(s) 40
TaiI ACGT 1 cut(s) 202
TasI AATT 1 cut(s) 136
TatI WGTACW 1 cut(s) 54
TfiI GAWTC 1 cut(s) 470
Tru1I TTAA 2 cut(s) 282, 413
Tru9I TTAA 2 cut(s) 282, 413
TscAI CASTG 2 cut(s) 109, 427
TseFI GTSAC 1 cut(s) 104
TseI GCWGC 2 cut(s) 24, 450
Tsp45I GTSAC 1 cut(s) 104
TspDTI ATGAA 2 cut(s) 17, 221
TspRI CASTG 2 cut(s) 109, 427
Tth111I GACNNNGTC 1 cut(s) 110
XmnI GAANNNNTTC 1 cut(s) 80
XspI CTAG 2 cut(s) 359, 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.