Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0004508

Physical Location & Seq

Genomic Coordinates Reverse (-)

19886874 .. 19894260

Gene Structure 7387 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1093800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2439 bp

ATGGCAATCGACGTCCAAGTTTTGAAAGAAAGGTATATTAATTCTTGCAGGAGGCACTGTGTCCTACCAAATCCTGCAATTCTGTCCATTCTCTTCAAGGCTAAGGTTAAAAAATATTGCCAGGAAGTATGTAGCTTGGAGATTTCATTAGACCACCTTAAGGATACTGATTTCCCGCCACTTCTTGATTTATGCATGGAGCTTGATGATTCAGAGGTTGAAGCAGTTGACATATGCAATAAATCATTGTATGTTCTGGATGGAAAATATGCTCTGTTGTTGATGCGCGCTATTAATCAAAAGCTTCGAGTTGTGGATCTCTATGATTTGTCATTTGGAAAGAATTTCTCGAGGGATCTTTCTCAGAGAGGTCTAACATGCCAAGTTTTAAACTTGAGGTCGTCGCATTTTCGGAAGCTCAACATGATGGGGGAGTTCATGCGGATACACACCCTTAACCTTGATTTTAGTACTAAGCTCACTAGTTTTCAGGAGGATTGTTTTACTTGCATGCCCAACCTAAAGTGTCTTTCTCTGTGTGAGACAAGAATTTCAAATCTCTGGACAACCATCGCCGTACTTTCTAAACTCCCTTCTTTGGTTGAACTTCGATTTCAGAATTGGTTGGGCTGCAATAATGTTGGCCCTTTTTCTGCATCATCTAGTGGGAAGCCTGATGACAAAACTGATTCCAATCTGCCAAATAGTGTTCATTATGGTGGGCGACCGTCCATGTGTGTTGGGGAACCAACCGATTACAATTCAAGCATTGAAGAGGTGCTACGGAATTTGTTTGTAGTGGGTAATATGGGTGTAAATGATGATGAACAAAACATGGTTGAAGATTCATCAGATGATAGTGAACTGGACTCACCGAATCCCCTGCAAGAACATGGTTCTGCAGGGCTATTATCAAATGTTTTCTCAGGGTGGAATAGACAGGCCGATCCGCAAAATGAGAACCAAAATGAAGAGGAGTCCTTGCAAGGTACCTTCACAAGGCATATTGGAGACATTGAATTGAAATATATTTCTTATCATGCTTCACCAATATGTTTTGAGAAACATTATAGAGAGTACATAATAGCCTCATTACCTCATTTGAGAAGTTTGGATAACTTGCCTATTGGAAAGATTGATCAGGAAAGGGCGAGGATTAAATTTTCAGAGTGTTTTGAGAATCTACCTTATCAGAGGAAGCATAAAGAGAATATTGTCCACATGTTACAGAAGCGTGAAATAAGAGCAAGCCAAACTCATGTGCAAAGTCCTGGTCAGAAATCATCAAATCTCTATGGAAAATCTCAACACTTCTATACAAGGTCTCTTTGTGCCGCTAAAATGGGATCTTCTGCCTGGCCTTTCTTGCACCCGCTATCGGTTTCAGGCAGTAACTTGGGAGGTGAAAGTAGGAGCTTTCGCCCTAGGCAGTTTGAGTATCATCCATCTGACTCTAGTCTAATGGTCTTTGGAACATTAGATGGTGAAGTAGTTGTAGTCAACCATGAGAATGAAAAAATTGTCAGTTATATCCCATCATCAGGAGCAATGAATAGTGTCCTGGGACTCTGTTGGCTCAAGAAGTATCCCTCTAAGCTAATAGCTGGGTCAGATAACGGTTCATTGAAATTATATGATATCCATCATACACGATCAATGGTAAGGGGCGTCTACCATAGTGCTGGTTCTGTGACTTTTGACGAGTTTGACCAGTTGACTTCTGTTCATGTTAACTCCACGGACGAACTATTTCTTGCTAGTGGATACTCAAGAGATGTTTCTTTGTATGACATAAGTAGTGGAAGACGCTTACAGGTGTTTACTGATATGCATCGGGAGCATATTAATGTACTGAAGTTTGCAAACCATTCCCCATCTCTTTTTGCGACTTCTTCATTTGATAAGGATGTCAAGATGTGGGATCTAAGACAGAAGCCAATACATCCTTGCTATACTTCTTCAAGCCCTAGGGGTAATGTGATGGTTTGCTTTTCTCCAGATGATCATTATCTTCTAGTGTCAGCTGTTGACAATAAGGTTCGACAACTTCTGGCAGTTGATGGGAGACTTCACTTGAATTTTGAGATAGCCTCTACAGGAAGCTCTCAGAATTACACCCGATCCTATTACATGAATGGAAGGGATTATATCATCAGCGGGAGTTGTGATGAGCATGTAGTCCGCATATGTTGTGCTCAAACTGGGAGGCGGCTGAGGGATATATCTTTGGAGGGACGAGGAGGATCTGGAAGTTCTATGTTTGTGCAATCGTTAAGGGGAGATCCTTTCAGAGAATTCAACATGAGCATCTTGGGAACATATATGCGTCCAAGCTCGAGGTCCGAAATTGTCAAGGTCAACATGCTAGCATCCAGTGACTATGCCAAGGAACACCTTGATGGTCAGCAGCCTTGCCCAACCAATAATATGGGAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006293 GO:0006294 GO:0006296 GO:0006325 GO:0006464 GO:0006508 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010390 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016579 GO:0019538 GO:0022607 GO:0030054 GO:0031334 GO:0031461 GO:0031465 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0033522 GO:0033554 GO:0033683 GO:0034622 GO:0034641 GO:0034644 GO:0035518 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043687 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051276 GO:0051716 GO:0051865 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070646 GO:0070647 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0080008 GO:0090304 GO:0090305 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

813

Amino Acids

91.62

Weight (kDa)

6.43

Isoelectric Point (pI)

50.6

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHU1_N	PF20919	5 - 388	2.9e-88	DWD hypersensitive to UV-B 1, N-terminal
WD40_Prp19	PF24814	478 - 681	2.1e-12	Prp19 WD40 domain
WD40_CDC20-Fz	PF24807	483 - 645	7.4e-11	CDC20/Fizzy WD40 domain
WD40_WDHD1_1st	PF24817	484 - 673	1.2e-06	WDHD1 first WD40 domain
Beta-prop_WDR5	PF25175	486 - 727	9.9e-18	WDR5 beta-propeller domain
Beta-prop_WDR36-Utp21_1st	PF25171	490 - 683	1.9e-13	WDR36/Utp21 first beta-propeller
Beta-prop_WDR3_1st	PF25173	492 - 681	3.7e-11	WDR3 first beta-propeller domain
Beta-prop_RIG_2nd	PF23775	523 - 670	3.5e-06	RIG second beta-propeller
WD40_CDC20-Fz	PF24807	569 - 681	1.4e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	572 - 723	1.1e-11	THOC3 beta-propeller domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0004508)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34280
fragaria_vesca	FvH4_2g04670 FvH4_2g04670
malus_domestica	MD05G1093800.v1.1
prunus_persica	Prupe.8G138700_v2.0.a1 Prupe.8G138700_v2.0.a1
pyrus_communis	pycom05g09290
rosa_chinensis	RchiOBHm_Chr6g0254291 RchiOBHm_Chr6g0254331 RchiOBHm_Chr6g0254371
rosa_laevigata	RLG00000014132 RLG00000014922 RLG00000014929
rosa_multiflora	Rmu_sc0012713.1_g000001 Rmu_sc0012713.1_g000002 Rmu_sc0027609.1_g000001 Rmu_ssc0000412.1_g000017
rosa_roxburghii	Rroxscaffold_7G00210950
rosa_rugosa	Rorug05G0551700 Rorug05G0551800 Rorug05G0551800
rosa_samantha	Rh6AG068100 Rh6AG068600 Rh6BG061000 Rh6BG061300 Rh6CG060800 Rh6CG061200 Rh6DG057900 Rh6DG058100 Rh6DG058500
rosa_wichuraiana	Rw6G005970 Rw6G006010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	15
Acc65I	GGTACC	1 cut(s)	989
AccB1I	GGYRCC	1 cut(s)	989
AccI	GTMKAC	1 cut(s)	1671
AccII	CGCG	1 cut(s)	288
AciI	CCGC	8 cut(s)	176, 442, 950, 1335, 1373, 2157, 2182, 2209
AclWI	GGATC	8 cut(s)	324, 363, 941, 1354, 1929, 2115, 2251, 2276
AcsI	RAATTY	6 cut(s)	343, 549, 787, 1160, 2077, 2294
AcuI	CTGAAG	1 cut(s)	1874
AcyI	GRCGYC	2 cut(s)	12, 1668
AfaI	GTAC	5 cut(s)	472, 579, 991, 1079, 1851
AfiI	CCNNNNNNNGG	5 cut(s)	160, 598, 999, 1378, 1541
AflII	CTTAAG	1 cut(s)	158
AflIII	ACRYGT	1 cut(s)	1221
AhlI	ACTAGT	1 cut(s)	482
AjnI	CCWGG	4 cut(s)	120, 1270, 1355, 1560
Alw21I	GWGCWC	1 cut(s)	2197
Alw26I	GTCTC	4 cut(s)	536, 1005, 1329, 2059
AlwI	GGATC	8 cut(s)	324, 363, 941, 1354, 1929, 2115, 2251, 2276
Ama87I	CYCGRG	2 cut(s)	349, 2335
AoxI	GGCC	3 cut(s)	643, 942, 1358
ApeKI	GCWGC	2 cut(s)	630, 2407
ApoI	RAATTY	6 cut(s)	343, 549, 787, 1160, 2077, 2294
ArsI	GACNNNNNNTTYG	2 cut(s)	1258, 1290
AseI	ATTAAT	3 cut(s)	39, 294, 1845
Asp700I	GAANNNNTTC	1 cut(s)	1749
Asp718I	GGTACC	1 cut(s)	989
AspA2I	CCTAGG	2 cut(s)	1424, 1967
AspLEI	GCGC	2 cut(s)	288, 290
AspS9I	GGNCC	2 cut(s)	644, 2340
AsuHPI	GGTGA	4 cut(s)	864, 1038, 1415, 1496
AsuNHI	GCTAGC	1 cut(s)	2365
AvaI	CYCGRG	2 cut(s)	349, 2335
AvaII	GGWCC	1 cut(s)	2340
AvrII	CCTAGG	2 cut(s)	1424, 1967
BanI	GGYRCC	1 cut(s)	989
BarI	GAAGNNNNNNTAC	2 cut(s)	765, 797
BbsI	GAAGAC	1 cut(s)	1810
Bbv12I	GWGCWC	1 cut(s)	2197
BbvCI	CCTCAGC	1 cut(s)	2213
BbvI	GCAGC	2 cut(s)	617, 2419
BceAI	ACGGC	1 cut(s)	560
BcgI	CGANNNNNNTGC	2 cut(s)	865, 899
BciT130I	CCWGG	4 cut(s)	122, 1272, 1357, 1562
BciVI	GTATCC	4 cut(s)	157, 438, 1596, 1757
BclI	TGATCA	2 cut(s)	1138, 2002
BcoDI	GTCTC	4 cut(s)	536, 1005, 1329, 2059
BcuI	ACTAGT	1 cut(s)	482
BfaI	CTAG	8 cut(s)	483, 663, 1425, 1455, 1758, 1968, 2015, 2366
BfmI	CTRYAG	2 cut(s)	900, 2094
BfrI	CTTAAG	1 cut(s)	158
BfuI	GTATCC	4 cut(s)	157, 438, 1596, 1757
BisI	GCNGC	4 cut(s)	631, 1335, 2210, 2408
BlnI	CCTAGG	2 cut(s)	1424, 1967
BlsI	GCNGC	4 cut(s)	632, 1336, 2211, 2409
BmcAI	AGTACT	1 cut(s)	472
Bme1390I	CCNGG	4 cut(s)	122, 1272, 1357, 1562
Bme18I	GGWCC	1 cut(s)	2340
BmeT110I	CYCGRG	2 cut(s)	349, 2335
BmgT120I	GGNCC	2 cut(s)	644, 2340
BmiI	GGNNCC	2 cut(s)	747, 991
BmrFI	CCNGG	4 cut(s)	122, 1272, 1357, 1562
BmrI	ACTGGG	1 cut(s)	2211
BmsI	GCATC	5 cut(s)	273, 665, 1840, 2316, 2378
BmtI	GCTAGC	1 cut(s)	2369
BmuI	ACTGGG	1 cut(s)	2211
BoxI	GACNNNNGTC	1 cut(s)	1455
BpiI	GAAGAC	1 cut(s)	1810
BplI	GAGNNNNNCTC	2 cut(s)	2075, 2107
BpmI	CTGGAG	1 cut(s)	1980
Bpu10I	CCTNAGC	2 cut(s)	102, 2213
BpuEI	CTTGAG	3 cut(s)	415, 1562, 1753
BsaBI	GATNNNNATC	4 cut(s)	693, 1641, 1830, 2007
BsaHI	GRCGYC	2 cut(s)	12, 1668
BsaI	GGTCTC	1 cut(s)	1329
BsaJI	CCNNGG	5 cut(s)	1424, 1561, 1737, 1967, 2385
BsaXI	ACNNNNNCTCC	2 cut(s)	43, 73
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 598, 999, 1378, 1541
Bse1I	ACTGG	4 cut(s)	870, 1711, 2206, 2373
Bse3DI	GCAATG	1 cut(s)	1554
Bse8I	GATNNNNATC	4 cut(s)	693, 1641, 1830, 2007
BseBI	CCWGG	4 cut(s)	122, 1272, 1357, 1562
BseDI	CCNNGG	5 cut(s)	1424, 1561, 1737, 1967, 2385
BseGI	GGATG	5 cut(s)	265, 1441, 1912, 1942, 2369
BseJI	GATNNNNATC	4 cut(s)	693, 1641, 1830, 2007
BseLI	CCNNNNNNNGG	5 cut(s)	160, 598, 999, 1378, 1541
BseMI	GCAATG	1 cut(s)	1554
BseMII	CTCAG	4 cut(s)	377, 939, 2120, 2204
BseNI	ACTGG	4 cut(s)	870, 1711, 2206, 2373
BsePI	GCGCGC	1 cut(s)	286
BseRI	GAGGAG	2 cut(s)	989, 2253
BseXI	GCAGC	2 cut(s)	617, 2419
BseYI	CCCAGC	1 cut(s)	1604
Bsh1236I	CGCG	1 cut(s)	288
Bsh1285I	CGRYCG	1 cut(s)	728
BshFI	GGCC	3 cut(s)	645, 944, 1360
BshNI	GGYRCC	1 cut(s)	989
BsiEI	CGRYCG	1 cut(s)	728
BsiHKAI	GWGCWC	1 cut(s)	2197
BsiHKCI	CYCGRG	2 cut(s)	349, 2335
BslFI	GGGAC	2 cut(s)	1578, 2247
BslI	CCNNNNNNNGG	5 cut(s)	160, 598, 999, 1378, 1541
BsmAI	GTCTC	4 cut(s)	536, 1005, 1329, 2059
BsmFI	GGGAC	2 cut(s)	1578, 2247
BsnI	GGCC	3 cut(s)	645, 944, 1360
Bso31I	GGTCTC	1 cut(s)	1329
BsoBI	CYCGRG	2 cut(s)	349, 2335
Bsp1286I	GDGCHC	1 cut(s)	2197
BspACI	CCGC	8 cut(s)	176, 442, 950, 1335, 1373, 2157, 2182, 2209
BspANI	GGCC	3 cut(s)	645, 944, 1360
BspCNI	CTCAG	4 cut(s)	376, 938, 2119, 2205
BspFNI	CGCG	1 cut(s)	288
BspLI	GGNNCC	2 cut(s)	747, 991
BspMAI	CTGCAG	1 cut(s)	904
BspOI	GCTAGC	1 cut(s)	2369
BspPI	GGATC	8 cut(s)	324, 363, 941, 1354, 1929, 2115, 2251, 2276
BspT107I	GGYRCC	1 cut(s)	989
BspTI	CTTAAG	1 cut(s)	158
BspTNI	GGTCTC	1 cut(s)	1329
BsrDI	GCAATG	1 cut(s)	1554
BsrI	ACTGG	4 cut(s)	870, 1711, 2206, 2373
BssECI	CCNNGG	5 cut(s)	1424, 1561, 1737, 1967, 2385
BssHII	GCGCGC	1 cut(s)	286
BssNI	GRCGYC	2 cut(s)	12, 1668
BssT1I	CCWWGG	3 cut(s)	1424, 1967, 2385
Bst2UI	CCWGG	4 cut(s)	122, 1272, 1357, 1562
Bst4CI	ACNGT	3 cut(s)	59, 729, 1619
Bst6I	CTCTTC	3 cut(s)	98, 768, 966
BstACI	GRCGYC	2 cut(s)	12, 1668
BstAFI	CTTAAG	1 cut(s)	158
BstC8I	GCNNGC	4 cut(s)	288, 512, 1249, 2367
BstDEI	CTNAG	8 cut(s)	102, 363, 474, 925, 1593, 1925, 2106, 2213
BstDSI	CCRYGG	1 cut(s)	1737
BstENI	CCTNNNNNAGG	1 cut(s)	997
BstF5I	GGATG	5 cut(s)	265, 1441, 1912, 1942, 2369
BstFNI	CGCG	1 cut(s)	288
BstHHI	GCGC	2 cut(s)	288, 290
BstMAI	GTCTC	4 cut(s)	536, 1005, 1329, 2059
BstMCI	CGRYCG	1 cut(s)	728
BstMWI	GCNNNNNNNGC	2 cut(s)	1366, 1837
BstNI	CCWGG	4 cut(s)	122, 1272, 1357, 1562
BstNSI	RCATGY	5 cut(s)	381, 514, 1225, 2177, 2365
BstPAI	GACNNNNGTC	1 cut(s)	1455
BstSCI	CCNGG	4 cut(s)	120, 1270, 1355, 1560
BstSFI	CTRYAG	2 cut(s)	900, 2094
BstUI	CGCG	1 cut(s)	288
BstV1I	GCAGC	2 cut(s)	617, 2419
BstV2I	GAAGAC	1 cut(s)	1810
BstX2I	RGATCY	6 cut(s)	316, 355, 1346, 1921, 2243, 2281
BstXI	CCANNNNNNTGG	2 cut(s)	1682, 2428
BstYI	RGATCY	6 cut(s)	316, 355, 1346, 1921, 2243, 2281
BsuI	GTATCC	4 cut(s)	157, 438, 1596, 1757
BsuRI	GGCC	3 cut(s)	645, 944, 1360
BtgI	CCRYGG	1 cut(s)	1737
BtgZI	GCGATG	1 cut(s)	556
BtsCI	GGATG	5 cut(s)	265, 1441, 1912, 1942, 2369
BtsIMutI	CAGTG	2 cut(s)	55, 2380
Cac8I	GCNNGC	4 cut(s)	288, 512, 1249, 2367
CfoI	GCGC	2 cut(s)	288, 290
Cfr13I	GGNCC	2 cut(s)	644, 2340
CseI	GACGC	3 cut(s)	1657, 1815, 2315
Csp6I	GTAC	5 cut(s)	471, 578, 990, 1078, 1850
CviQI	GTAC	5 cut(s)	471, 578, 990, 1078, 1850
DdeI	CTNAG	8 cut(s)	102, 363, 474, 925, 1593, 1925, 2106, 2213
DraI	TTTAAA	1 cut(s)	390
Eam1104I	CTCTTC	3 cut(s)	98, 768, 966
EarI	CTCTTC	3 cut(s)	98, 768, 966
Eco130I	CCWWGG	3 cut(s)	1424, 1967, 2385
Eco31I	GGTCTC	1 cut(s)	1329
Eco32I	GATATC	1 cut(s)	1639
Eco47I	GGWCC	1 cut(s)	2340
Eco57I	CTGAAG	1 cut(s)	1874
Eco88I	CYCGRG	2 cut(s)	349, 2335
EcoNI	CCTNNNNNAGG	1 cut(s)	997
EcoRI	GAATTC	1 cut(s)	2294
EcoRII	CCWGG	4 cut(s)	120, 1270, 1355, 1560
EcoRV	GATATC	1 cut(s)	1639
EcoT14I	CCWWGG	3 cut(s)	1424, 1967, 2385
EcoT22I	ATGCAT	2 cut(s)	197, 1833
ErhI	CCWWGG	3 cut(s)	1424, 1967, 2385
FaqI	GGGAC	2 cut(s)	1578, 2247
FauI	CCCGC	3 cut(s)	183, 1380, 2150
FauNDI	CATATG	2 cut(s)	233, 2186
FbaI	TGATCA	2 cut(s)	1138, 2002
FblI	GTMKAC	1 cut(s)	1671
Fnu4HI	GCNGC	4 cut(s)	631, 1335, 2210, 2408
FokI	GGATG	5 cut(s)	272, 1428, 1919, 1929, 2356
Fsp4HI	GCNGC	4 cut(s)	631, 1335, 2210, 2408
FspBI	CTAG	8 cut(s)	483, 663, 1425, 1455, 1758, 1968, 2015, 2366
GlaI	GCGC	2 cut(s)	287, 289
GluI	GCNGC	4 cut(s)	631, 1335, 2210, 2408
GsaI	CCCAGC	1 cut(s)	1608
GsuI	CTGGAG	1 cut(s)	1980
HaeIII	GGCC	3 cut(s)	645, 944, 1360
HgaI	GACGC	3 cut(s)	1657, 1815, 2315
HhaI	GCGC	2 cut(s)	288, 290
Hin1I	GRCGYC	2 cut(s)	12, 1668
Hin6I	GCGC	2 cut(s)	286, 288
HinP1I	GCGC	2 cut(s)	286, 288
HincII	GTYRAC	6 cut(s)	229, 1501, 1716, 1732, 2029, 2359
HindII	GTYRAC	6 cut(s)	229, 1501, 1716, 1732, 2029, 2359
HindIII	AAGCTT	1 cut(s)	302
HinfI	GANTC	9 cut(s)	209, 689, 845, 869, 877, 977, 1180, 1451, 1566
HpaI	GTTAAC	1 cut(s)	1732
HphI	GGTGA	4 cut(s)	864, 1038, 1415, 1496
Hpy99I	CGWCG	2 cut(s)	14, 406
HpyAV	CCTTC	3 cut(s)	603, 1003, 2133
HpyCH4III	ACNGT	3 cut(s)	59, 729, 1619
HpyCH4IV	ACGT	1 cut(s)	12
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1366, 1837
HpyF3I	CTNAG	8 cut(s)	102, 363, 474, 925, 1593, 1925, 2106, 2213
HpySE526I	ACGT	1 cut(s)	12
Hsp92I	GRCGYC	2 cut(s)	12, 1668
HspAI	GCGC	2 cut(s)	286, 288
KpnI	GGTACC	1 cut(s)	993
Ksp22I	TGATCA	2 cut(s)	1138, 2002
KspAI	GTTAAC	1 cut(s)	1732
LmnI	GCTCC	4 cut(s)	199, 1413, 1544, 1837
Lsp1109I	GCAGC	2 cut(s)	617, 2419
LweI	GCATC	5 cut(s)	273, 665, 1840, 2316, 2378
MaeI	CTAG	8 cut(s)	483, 663, 1425, 1455, 1758, 1968, 2015, 2366
MaeII	ACGT	1 cut(s)	12
MaeIII	GTNAC	4 cut(s)	1224, 1391, 1690, 2375
MboII	GAAGA	9 cut(s)	85, 785, 854, 983, 1341, 1815, 1884, 1950, 2003
MflI	RGATCY	6 cut(s)	316, 355, 1346, 1921, 2243, 2281
MhlI	GDGCHC	1 cut(s)	2197
MlyI	GAGTC	4 cut(s)	863, 986, 1445, 1560
Mph1103I	ATGCAT	2 cut(s)	197, 1833
MroXI	GAANNNNTTC	1 cut(s)	1749
MslI	CAYNNNNRTG	5 cut(s)	717, 1051, 1509, 1845, 2397
MspA1I	CMGCKG	2 cut(s)	2024, 2157
MspCI	CTTAAG	1 cut(s)	158
MspR9I	CCNGG	4 cut(s)	122, 1272, 1357, 1562
MvaI	CCWGG	4 cut(s)	122, 1272, 1357, 1562
MvnI	CGCG	1 cut(s)	288
MwoI	GCNNNNNNNGC	2 cut(s)	1366, 1837
NdeI	CATATG	2 cut(s)	233, 2186
NheI	GCTAGC	1 cut(s)	2365
NlaIV	GGNNCC	2 cut(s)	747, 991
NmuCI	GTSAC	2 cut(s)	1690, 2375
NsiI	ATGCAT	2 cut(s)	197, 1833
NspI	RCATGY	5 cut(s)	381, 514, 1225, 2177, 2365
PaeI	GCATGC	1 cut(s)	514
PaeR7I	CTCGAG	2 cut(s)	349, 2335
PauI	GCGCGC	1 cut(s)	286
PciI	ACATGT	1 cut(s)	1221
PdmI	GAANNNNTTC	1 cut(s)	1749
PfeI	GAWTC	5 cut(s)	209, 689, 845, 877, 1180
PkrI	GCNGC	4 cut(s)	632, 1336, 2211, 2409
PleI	GAGTC	4 cut(s)	863, 985, 1445, 1560
PpsI	GAGTC	4 cut(s)	863, 985, 1445, 1560
PscI	ACATGT	1 cut(s)	1221
PshAI	GACNNNNGTC	1 cut(s)	1455
PshBI	ATTAAT	3 cut(s)	39, 294, 1845
Psp6I	CCWGG	4 cut(s)	120, 1270, 1355, 1560
PspFI	CCCAGC	1 cut(s)	1604
PspGI	CCWGG	4 cut(s)	120, 1270, 1355, 1560
PspN4I	GGNNCC	2 cut(s)	747, 991
PspPI	GGNCC	2 cut(s)	644, 2340
PspXI	VCTCGAGB	1 cut(s)	2335
PstI	CTGCAG	1 cut(s)	904
PsuI	RGATCY	6 cut(s)	316, 355, 1346, 1921, 2243, 2281
PteI	GCGCGC	1 cut(s)	286
PvuII	CAGCTG	1 cut(s)	2024
RsaI	GTAC	5 cut(s)	472, 579, 991, 1079, 1851
RsaNI	GTAC	5 cut(s)	471, 578, 990, 1078, 1850
RseI	CAYNNNNRTG	5 cut(s)	717, 1051, 1509, 1845, 2397
SatI	GCNGC	4 cut(s)	631, 1335, 2210, 2408
Sau96I	GGNCC	2 cut(s)	644, 2340
ScaI	AGTACT	1 cut(s)	472
SchI	GAGTC	4 cut(s)	863, 986, 1445, 1560
ScrFI	CCNGG	4 cut(s)	122, 1272, 1357, 1562
SduI	GDGCHC	1 cut(s)	2197
SfaNI	GCATC	5 cut(s)	273, 665, 1840, 2316, 2378
SfcI	CTRYAG	2 cut(s)	900, 2094
Sfr274I	CTCGAG	2 cut(s)	349, 2335
SinI	GGWCC	1 cut(s)	2340
SlaI	CTCGAG	2 cut(s)	349, 2335
SmiMI	CAYNNNNRTG	5 cut(s)	717, 1051, 1509, 1845, 2397
SmlI	CTYRAG	6 cut(s)	158, 349, 394, 1577, 1768, 2335
SmoI	CTYRAG	6 cut(s)	158, 349, 394, 1577, 1768, 2335
SpeI	ACTAGT	1 cut(s)	482
SphI	GCATGC	1 cut(s)	514
SsiI	CCGC	8 cut(s)	176, 442, 950, 1335, 1373, 2157, 2182, 2209
SspI	AATATT	2 cut(s)	116, 1213
SspMI	CTAG	8 cut(s)	483, 663, 1425, 1455, 1758, 1968, 2015, 2366
StyD4I	CCNGG	4 cut(s)	120, 1270, 1355, 1560
StyI	CCWWGG	3 cut(s)	1424, 1967, 2385
TaaI	ACNGT	3 cut(s)	59, 729, 1619
TaiI	ACGT	1 cut(s)	15
TaqI	TCGA	6 cut(s)	9, 307, 350, 610, 2041, 2336
TatI	WGTACW	3 cut(s)	470, 1077, 1849
TauI	GCSGC	2 cut(s)	1337, 2212
TfiI	GAWTC	5 cut(s)	209, 689, 845, 877, 1180
TscAI	CASTG	2 cut(s)	62, 2380
TseFI	GTSAC	2 cut(s)	1690, 2375
TseI	GCWGC	2 cut(s)	630, 2407
Tsp45I	GTSAC	2 cut(s)	1690, 2375
TspGWI	ACGGA	2 cut(s)	799, 1754
TspRI	CASTG	2 cut(s)	62, 2380
Vha464I	CTTAAG	1 cut(s)	158
VpaK11BI	GGWCC	1 cut(s)	2340
VspI	ATTAAT	3 cut(s)	39, 294, 1845
XagI	CCTNNNNNAGG	1 cut(s)	997
XapI	RAATTY	6 cut(s)	343, 549, 787, 1160, 2077, 2294
XceI	RCATGY	5 cut(s)	381, 514, 1225, 2177, 2365
XhoI	CTCGAG	2 cut(s)	349, 2335
XmaJI	CCTAGG	2 cut(s)	1424, 1967
XmiI	GTMKAC	1 cut(s)	1671
XmnI	GAANNNNTTC	1 cut(s)	1749
XspI	CTAG	8 cut(s)	483, 663, 1425, 1455, 1758, 1968, 2015, 2366
ZraI	GACGTC	1 cut(s)	13
ZrmI	AGTACT	1 cut(s)	472
Zsp2I	ATGCAT	2 cut(s)	197, 1833

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.