Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004508

Physical Location & Seq

Genomic Coordinates Forward (+)

9366350 .. 9371727

Gene Structure 5378 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ22809

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1479 bp

ATGAGCTTACTGCTTTCATTGGTGGATTCTCTTGGTAACTTTCAGAATCAAAATGAGGCAGAGCCTTTAGCTGGTCCCTTCTCAAAGTATATTGGAGACAATTCAGTGAAATATATTTCTTATCATGCTTCGCCAATATGTTTTGAGAAATATTATAGAGAGTACATGATAGCCTCATTACCTCGTTTGAGAATTTTAGATAACTTGCAAATTAGAAAGATTGATCAGGAAACTGCTAGGATGACATTTGCAGAATATTTTGAGCATGTACCATATCAAAGAAAGCCTAACGAAAATGTCGTTAGTGTGTTACAAAAGCGTGAAATAAGATCAAGCCAAAATCATGTACAAAGTCCTGGGAAAAAAGTATCGAATCTCTATGGAAAGTCTCAATGCTTCTATACCAGGTCTCTTTCTGCTGCCAAAATGGGATCTTGTGTTTGGCCATTCTTGAACCCGCTTTCTTTTTCAGGCAGTACTCTGGTAGGTGAAAGTAAGAGCTTTCGTCCTAGGCAGTTTGAGTATCATCCATCCAACTCTAGCCTTATGGTCTTTGGAACATTAGATGGTGAAATAGTTGCAGTCAACCATGAGAATGGGAAAATTGTCAGTTATATCCCATCACTAGGAGCAATGAATAGTGTGCTGGGACTCTTTTGGCTCAAGAAGTATCCCTCTAAGAAATTAACTAATAGCTGGTTCAGAACTTCAGATAATGGCTCATTGAAATTGTACGACATCCATCATATGCGATCAATGGAAACAGGCATATATGGTGGTGCCGGGTCTGTCTCCTTTGACGAGTTTGACCAGTTGACATCTGTTCATGTTAATTCCACGGACGAGCTATTTCTTGCTAGTGGATACTCAAGAGACATTGCCTTATATGACATTAGTAGTGGAAAGCGCTTACAGGTGTTTACTAATATGCATCGGGAGCATATTAATGTAATAAAGTTTGCAAACCATTCCCCATCTGTTTTCGCCACTTCTTCATTTGATCAGGATGTCAAGATGTGGGACCTAAGACAGAAACCAATAAGCCCTTGCTATTCCTCTTCAAGTTCTAGGGGTAATGTGATGGTTTGCTTTTCTCCTGATGATCACTATCTTCTTGTCTCAGCTGTTGATAACGAGGTTAGGCAACTTCTCGCAGTTGATGGGAGACTTCACTTGAATTTTGAGATAGCCTCTACAGGAAGTTCTCAGAACTACACTCGATCCTATTACATGAATGGAAGAGATTATATCATCAGTGGGAGTTGTGATGAACATGTTGTCCGCATATGCTGTGCTCAAACAGGGAGGCGCCTGAGGGATATATCTTTGGAGGGAGGAGGAAGATCTAGGAGTTCTATGTTTGTGCAATCTTTGAGGGGTGATCCTTTCAGAGAATTCAACATGAGTATCTTGGCAGCCCATATGCGTCCAAGATTGAAGTCTGAAATTATCAAGGTCAATCTGCTAGCTAGCATCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006293 GO:0006294 GO:0006296 GO:0006325 GO:0006464 GO:0006508 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010390 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016579 GO:0019538 GO:0022607 GO:0030054 GO:0031334 GO:0031461 GO:0031465 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0033522 GO:0033554 GO:0033683 GO:0034622 GO:0034641 GO:0034644 GO:0035518 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043687 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051276 GO:0051716 GO:0051865 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070646 GO:0070647 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0080008 GO:0090304 GO:0090305 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

492

Amino Acids

55.67

Weight (kDa)

8.99

Isoelectric Point (pI)

46.07

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHU1_N	PF20919	26 - 83	4.5e-15	DWD hypersensitive to UV-B 1, N-terminal
WD40_Prp19	PF24814	173 - 381	4.2e-10	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	237 - 383	1.9e-08	WDR36/Utp21 first beta-propeller
WD40_CDC20-Fz	PF24807	269 - 381	8.3e-07	CDC20/Fizzy WD40 domain
Beta-prop_WDR5	PF25175	271 - 427	1.4e-15	WDR5 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	271 - 381	5.5e-09	WDR3 first beta-propeller domain
Beta-prop_THOC3	PF25174	272 - 424	1.9e-11	THOC3 beta-propeller domain
WD40_WDHD1_1st	PF24817	272 - 427	1.6e-06	WDHD1 first WD40 domain
WDR55	PF24796	284 - 421	4.9e-06	WDR55

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004508)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34280
fragaria_vesca	FvH4_2g04670 FvH4_2g04670
malus_domestica	MD05G1093800.v1.1
prunus_persica	Prupe.8G138700_v2.0.a1 Prupe.8G138700_v2.0.a1
pyrus_communis	pycom05g09290
rosa_chinensis	RchiOBHm_Chr6g0254291 RchiOBHm_Chr6g0254331 RchiOBHm_Chr6g0254371
rosa_laevigata	RLG00000014132 RLG00000014922 RLG00000014929
rosa_multiflora	Rmu_sc0012713.1_g000001 Rmu_sc0012713.1_g000002 Rmu_sc0027609.1_g000001 Rmu_ssc0000412.1_g000017
rosa_roxburghii	Rroxscaffold_7G00210950
rosa_rugosa	Rorug05G0551700 Rorug05G0551800 Rorug05G0551800
rosa_samantha	Rh6AG068100 Rh6AG068600 Rh6BG061000 Rh6BG061300 Rh6CG060800 Rh6CG061200 Rh6DG057900 Rh6DG058100 Rh6DG058500
rosa_wichuraiana	Rw6G005970 Rw6G006010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	779, 1308
AciI	CCGC	2 cut(s)	458, 1282
AclWI	GGATC	3 cut(s)	439, 1215, 1376
AcoI	YGGCCR	1 cut(s)	443
AcsI	RAATTY	3 cut(s)	192, 1177, 1394
AcuI	CTGAAG	1 cut(s)	693
AcyI	GRCGYC	1 cut(s)	1309
AfaI	GTAC	5 cut(s)	164, 270, 348, 478, 734
AfeI	AGCGCT	1 cut(s)	908
AfiI	CCNNNNNNNGG	2 cut(s)	71, 626
AflIII	ACRYGT	1 cut(s)	1273
AgsI	TTSAA	6 cut(s)	454, 727, 1062, 1177, 1399, 1438
AjnI	CCWGG	2 cut(s)	355, 404
AloI	GAACNNNNNNTCC	2 cut(s)	1263, 1295
AluBI	AGCT	7 cut(s)	6, 71, 501, 696, 847, 1124, 1469
AluI	AGCT	7 cut(s)	6, 71, 501, 696, 847, 1124, 1469
Alw21I	GWGCWC	1 cut(s)	1297
Alw26I	GTCTC	7 cut(s)	90, 393, 414, 796, 867, 1123, 1159
AlwI	GGATC	3 cut(s)	439, 1215, 1376
Aor51HI	AGCGCT	1 cut(s)	908
AoxI	GGCC	1 cut(s)	443
ApeKI	GCWGC	2 cut(s)	419, 1415
ApoI	RAATTY	3 cut(s)	192, 1177, 1394
AseI	ATTAAT	1 cut(s)	945
AspA2I	CCTAGG	1 cut(s)	509
AspLEI	GCGC	2 cut(s)	909, 1311
AspS9I	GGNCC	2 cut(s)	74, 1021
AsuC2I	CCSGG	1 cut(s)	784
AsuHPI	GGTGA	3 cut(s)	500, 581, 1391
AsuNHI	GCTAGC	2 cut(s)	1465, 1469
AvaII	GGWCC	2 cut(s)	74, 1021
AvrII	CCTAGG	1 cut(s)	509
AxyI	CCTNAGG	1 cut(s)	1313
BaeI	ACNNNNGTAYC	2 cut(s)	1390, 1423
BalI	TGGCCA	1 cut(s)	445
BanI	GGYRCC	2 cut(s)	779, 1308
Bbv12I	GWGCWC	1 cut(s)	1297
BbvI	GCAGC	2 cut(s)	406, 1427
BccI	CCATC	7 cut(s)	538, 560, 628, 750, 982, 1075, 1154
BciT130I	CCWGG	2 cut(s)	357, 406
BciVI	GTATCC	2 cut(s)	681, 857
BclI	TGATCA	3 cut(s)	223, 1000, 1102
BcnI	CCSGG	1 cut(s)	784
BcoDI	GTCTC	7 cut(s)	90, 393, 414, 796, 867, 1123, 1159
BfmI	CTRYAG	1 cut(s)	1194
BfoI	RGCGCY	2 cut(s)	910, 1312
BfuI	GTATCC	2 cut(s)	681, 857
BglII	AGATCT	1 cut(s)	1343
BisI	GCNGC	2 cut(s)	420, 1416
BlnI	CCTAGG	1 cut(s)	509
BlsI	GCNGC	2 cut(s)	421, 1417
BmcAI	AGTACT	1 cut(s)	478
Bme1390I	CCNGG	3 cut(s)	357, 406, 784
Bme18I	GGWCC	2 cut(s)	74, 1021
BmgT120I	GGNCC	2 cut(s)	74, 1021
BmiI	GGNNCC	4 cut(s)	76, 781, 1022, 1310
BmrFI	CCNGG	3 cut(s)	357, 406, 784
BmsI	GCATC	1 cut(s)	940
BmtI	GCTAGC	2 cut(s)	1469, 1473
BplI	GAGNNNNNCTC	2 cut(s)	1175, 1207
BpuEI	CTTGAG	2 cut(s)	647, 853
BpuMI	CCSGG	1 cut(s)	784
BsaBI	GATNNNNATC	1 cut(s)	1107
BsaHI	GRCGYC	1 cut(s)	1309
BsaI	GGTCTC	1 cut(s)	414
BsaJI	CCNNGG	3 cut(s)	356, 509, 837
Bsc4I	CCNNNNNNNGG	2 cut(s)	71, 626
Bse1I	ACTGG	1 cut(s)	811
Bse21I	CCTNAGG	1 cut(s)	1313
Bse3DI	GCAATG	2 cut(s)	639, 876
Bse8I	GATNNNNATC	1 cut(s)	1107
BseBI	CCWGG	2 cut(s)	357, 406
BseDI	CCNNGG	3 cut(s)	356, 509, 837
BseGI	GGATG	5 cut(s)	246, 526, 530, 738, 1012
BseJI	GATNNNNATC	1 cut(s)	1107
BseLI	CCNNNNNNNGG	2 cut(s)	71, 626
BseMI	GCAATG	2 cut(s)	639, 876
BseMII	CTCAG	3 cut(s)	1134, 1220, 1304
BseNI	ACTGG	1 cut(s)	811
BseRI	GAGGAG	1 cut(s)	1350
BseXI	GCAGC	2 cut(s)	406, 1427
BseYI	CCCAGC	1 cut(s)	646
BshFI	GGCC	1 cut(s)	445
BshNI	GGYRCC	2 cut(s)	779, 1308
BsiHKAI	GWGCWC	1 cut(s)	1297
BsiSI	CCGG	1 cut(s)	783
BslFI	GGGAC	3 cut(s)	60, 663, 1034
BslI	CCNNNNNNNGG	2 cut(s)	71, 626
BsmAI	GTCTC	7 cut(s)	90, 393, 414, 796, 867, 1123, 1159
BsmFI	GGGAC	3 cut(s)	60, 663, 1034
BsnI	GGCC	1 cut(s)	445
Bso31I	GGTCTC	1 cut(s)	414
Bsp1286I	GDGCHC	1 cut(s)	1297
Bsp1407I	TGTACA	1 cut(s)	346
Bsp143I	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
BspACI	CCGC	2 cut(s)	458, 1282
BspANI	GGCC	1 cut(s)	445
BspCNI	CTCAG	3 cut(s)	1133, 1219, 1305
BspLI	GGNNCC	4 cut(s)	76, 781, 1022, 1310
BspOI	GCTAGC	2 cut(s)	1469, 1473
BspPI	GGATC	3 cut(s)	439, 1215, 1376
BspT107I	GGYRCC	2 cut(s)	779, 1308
BspTNI	GGTCTC	1 cut(s)	414
BsrDI	GCAATG	2 cut(s)	639, 876
BsrGI	TGTACA	1 cut(s)	346
BsrI	ACTGG	1 cut(s)	811
BssECI	CCNNGG	3 cut(s)	356, 509, 837
BssMI	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
BssNI	GRCGYC	1 cut(s)	1309
BssT1I	CCWWGG	1 cut(s)	509
Bst2UI	CCWGG	2 cut(s)	357, 406
Bst6I	CTCTTC	2 cut(s)	1063, 1234
BstACI	GRCGYC	1 cut(s)	1309
BstAUI	TGTACA	1 cut(s)	346
BstC8I	GCNNGC	2 cut(s)	1467, 1471
BstDEI	CTNAG	5 cut(s)	678, 1025, 1120, 1206, 1313
BstDSI	CCRYGG	1 cut(s)	837
BstF5I	GGATG	5 cut(s)	246, 526, 530, 738, 1012
BstH2I	RGCGCY	2 cut(s)	910, 1312
BstHHI	GCGC	2 cut(s)	909, 1311
BstKTI	GATC	9 cut(s)	226, 332, 434, 755, 1003, 1105, 1223, 1346, 1384
BstMAI	GTCTC	7 cut(s)	90, 393, 414, 796, 867, 1123, 1159
BstMBI	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
BstMWI	GCNNNNNNNGC	1 cut(s)	937
BstNI	CCWGG	2 cut(s)	357, 406
BstNSI	RCATGY	2 cut(s)	269, 1277
BstSCI	CCNGG	3 cut(s)	355, 404, 782
BstSFI	CTRYAG	1 cut(s)	1194
BstV1I	GCAGC	2 cut(s)	406, 1427
BstX2I	RGATCY	2 cut(s)	431, 1343
BstXI	CCANNNNNNTGG	1 cut(s)	596
BstYI	RGATCY	2 cut(s)	431, 1343
Bsu36I	CCTNAGG	1 cut(s)	1313
BsuI	GTATCC	2 cut(s)	681, 857
BsuRI	GGCC	1 cut(s)	445
BtgI	CCRYGG	1 cut(s)	837
BtsCI	GGATG	5 cut(s)	246, 526, 530, 738, 1012
BtsIMutI	CAGTG	2 cut(s)	111, 1261
Cac8I	GCNNGC	2 cut(s)	1467, 1471
CfoI	GCGC	2 cut(s)	909, 1311
Cfr13I	GGNCC	2 cut(s)	74, 1021
CseI	GACGC	1 cut(s)	1415
CsiI	ACCWGGT	1 cut(s)	404
Csp6I	GTAC	5 cut(s)	163, 269, 347, 477, 733
CviAII	CATG	9 cut(s)	125, 166, 266, 344, 590, 827, 1231, 1274, 1402
CviQI	GTAC	5 cut(s)	163, 269, 347, 477, 733
DdeI	CTNAG	5 cut(s)	678, 1025, 1120, 1206, 1313
DinI	GGCGCC	1 cut(s)	1310
DpnI	GATC	9 cut(s)	225, 331, 433, 754, 1002, 1104, 1222, 1345, 1383
DpnII	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
EaeI	YGGCCR	1 cut(s)	443
Eam1104I	CTCTTC	2 cut(s)	1063, 1234
EarI	CTCTTC	2 cut(s)	1063, 1234
Eco130I	CCWWGG	1 cut(s)	509
Eco31I	GGTCTC	1 cut(s)	414
Eco47I	GGWCC	2 cut(s)	74, 1021
Eco47III	AGCGCT	1 cut(s)	908
Eco57I	CTGAAG	1 cut(s)	693
Eco81I	CCTNAGG	1 cut(s)	1313
EcoO109I	RGGNCCY	1 cut(s)	1021
EcoRI	GAATTC	1 cut(s)	1394
EcoRII	CCWGG	2 cut(s)	355, 404
EcoT14I	CCWWGG	1 cut(s)	509
EcoT22I	ATGCAT	1 cut(s)	933
EgeI	GGCGCC	1 cut(s)	1310
EheI	GGCGCC	1 cut(s)	1310
ErhI	CCWWGG	1 cut(s)	509
FaeI	CATG	9 cut(s)	128, 169, 269, 347, 593, 830, 1234, 1277, 1405
FaqI	GGGAC	3 cut(s)	60, 663, 1034
FatI	CATG	9 cut(s)	124, 165, 265, 343, 589, 826, 1230, 1273, 1401
FauI	CCCGC	1 cut(s)	465
FauNDI	CATATG	3 cut(s)	747, 1286, 1422
FbaI	TGATCA	3 cut(s)	223, 1000, 1102
Fnu4HI	GCNGC	2 cut(s)	420, 1416
FokI	GGATG	5 cut(s)	253, 513, 517, 725, 1019
Fsp4HI	GCNGC	2 cut(s)	420, 1416
GlaI	GCGC	2 cut(s)	908, 1310
GluI	GCNGC	2 cut(s)	420, 1416
GsaI	CCCAGC	1 cut(s)	650
HaeII	RGCGCY	2 cut(s)	910, 1312
HaeIII	GGCC	1 cut(s)	445
HapII	CCGG	1 cut(s)	783
HgaI	GACGC	1 cut(s)	1415
HhaI	GCGC	2 cut(s)	909, 1311
Hin1I	GRCGYC	1 cut(s)	1309
Hin1II	CATG	9 cut(s)	128, 169, 269, 347, 593, 830, 1234, 1277, 1405
Hin6I	GCGC	2 cut(s)	907, 1309
HinP1I	GCGC	2 cut(s)	907, 1309
HincII	GTYRAC	2 cut(s)	586, 816
HindII	GTYRAC	2 cut(s)	586, 816
HinfI	GANTC	4 cut(s)	26, 46, 373, 651
HpaII	CCGG	1 cut(s)	783
HphI	GGTGA	3 cut(s)	500, 581, 1391
Hpy166II	GTNNAC	3 cut(s)	586, 816, 921
Hpy188I	TCNGA	6 cut(s)	45, 704, 712, 1209, 1391, 1444
Hpy188III	TCNNGA	8 cut(s)	227, 451, 664, 870, 935, 1004, 1012, 1097
Hpy8I	GTNNAC	3 cut(s)	586, 816, 921
HpyAV	CCTTC	1 cut(s)	88
HpyCH4V	TGCA	6 cut(s)	208, 251, 581, 931, 962, 1366
HpyF10VI	GCNNNNNNNGC	1 cut(s)	937
HpyF3I	CTNAG	5 cut(s)	678, 1025, 1120, 1206, 1313
Hsp92I	GRCGYC	1 cut(s)	1309
Hsp92II	CATG	9 cut(s)	128, 169, 269, 347, 593, 830, 1234, 1277, 1405
HspAI	GCGC	2 cut(s)	907, 1309
KasI	GGCGCC	1 cut(s)	1308
Ksp22I	TGATCA	3 cut(s)	223, 1000, 1102
Kzo9I	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
LmnI	GCTCC	2 cut(s)	629, 937
Lsp1109I	GCAGC	2 cut(s)	406, 1427
LweI	GCATC	1 cut(s)	940
MabI	ACCWGGT	1 cut(s)	404
MaeIII	GTNAC	2 cut(s)	35, 309
MalI	GATC	9 cut(s)	225, 331, 433, 754, 1002, 1104, 1222, 1345, 1383
MboI	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
MboII	GAAGA	5 cut(s)	984, 1050, 1103, 1251, 1353
MflI	RGATCY	2 cut(s)	431, 1343
MhlI	GDGCHC	1 cut(s)	1297
MlsI	TGGCCA	1 cut(s)	445
MluNI	TGGCCA	1 cut(s)	445
Mly113I	GGCGCC	1 cut(s)	1309
MlyI	GAGTC	1 cut(s)	645
MmeI	TCCRAC	1 cut(s)	558
Mox20I	TGGCCA	1 cut(s)	445
Mph1103I	ATGCAT	1 cut(s)	933
MscI	TGGCCA	1 cut(s)	445
MseI	TTAA	3 cut(s)	686, 831, 945
MslI	CAYNNNNRTG	2 cut(s)	594, 945
Msp20I	TGGCCA	1 cut(s)	445
MspA1I	CMGCKG	1 cut(s)	1124
MspI	CCGG	1 cut(s)	783
MspR9I	CCNGG	3 cut(s)	357, 406, 784
MvaI	CCWGG	2 cut(s)	357, 406
MwoI	GCNNNNNNNGC	1 cut(s)	937
NarI	GGCGCC	1 cut(s)	1309
NciI	CCSGG	1 cut(s)	784
NdeI	CATATG	3 cut(s)	747, 1286, 1422
NdeII	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
NheI	GCTAGC	2 cut(s)	1465, 1469
NlaIII	CATG	9 cut(s)	128, 169, 269, 347, 593, 830, 1234, 1277, 1405
NlaIV	GGNNCC	4 cut(s)	76, 781, 1022, 1310
NsiI	ATGCAT	1 cut(s)	933
NspI	RCATGY	2 cut(s)	269, 1277
PciI	ACATGT	1 cut(s)	1273
PcsI	WCGNNNNNNNCGW	1 cut(s)	297
PfeI	GAWTC	3 cut(s)	26, 46, 373
PkrI	GCNGC	2 cut(s)	421, 1417
PleI	GAGTC	1 cut(s)	645
PluTI	GGCGCC	1 cut(s)	1312
PpsI	GAGTC	1 cut(s)	645
PpuMI	RGGWCCY	1 cut(s)	1021
PscI	ACATGT	1 cut(s)	1273
PshBI	ATTAAT	1 cut(s)	945
Psp5II	RGGWCCY	1 cut(s)	1021
Psp6I	CCWGG	2 cut(s)	355, 404
PspFI	CCCAGC	1 cut(s)	646
PspGI	CCWGG	2 cut(s)	355, 404
PspN4I	GGNNCC	4 cut(s)	76, 781, 1022, 1310
PspPI	GGNCC	2 cut(s)	74, 1021
PspPPI	RGGWCCY	1 cut(s)	1021
PsuI	RGATCY	2 cut(s)	431, 1343
PvuII	CAGCTG	1 cut(s)	1124
RsaI	GTAC	5 cut(s)	164, 270, 348, 478, 734
RsaNI	GTAC	5 cut(s)	163, 269, 347, 477, 733
RseI	CAYNNNNRTG	2 cut(s)	594, 945
SaqAI	TTAA	3 cut(s)	686, 831, 945
SatI	GCNGC	2 cut(s)	420, 1416
Sau3AI	GATC	9 cut(s)	223, 329, 431, 752, 1000, 1102, 1220, 1343, 1381
Sau96I	GGNCC	2 cut(s)	74, 1021
ScaI	AGTACT	1 cut(s)	478
SchI	GAGTC	1 cut(s)	645
ScrFI	CCNGG	3 cut(s)	357, 406, 784
SduI	GDGCHC	1 cut(s)	1297
SexAI	ACCWGGT	1 cut(s)	404
SfaNI	GCATC	1 cut(s)	940
SfcI	CTRYAG	1 cut(s)	1194
SfoI	GGCGCC	1 cut(s)	1310
SinI	GGWCC	2 cut(s)	74, 1021
SmiMI	CAYNNNNRTG	2 cut(s)	594, 945
SmlI	CTYRAG	2 cut(s)	662, 868
SmoI	CTYRAG	2 cut(s)	662, 868
SsiI	CCGC	2 cut(s)	458, 1282
SspDI	GGCGCC	1 cut(s)	1308
SspI	AATATT	2 cut(s)	152, 257
StyD4I	CCNGG	3 cut(s)	355, 404, 782
StyI	CCWWGG	1 cut(s)	509
TaqI	TCGA	2 cut(s)	371, 1219
TatI	WGTACW	3 cut(s)	162, 346, 476
TfiI	GAWTC	3 cut(s)	26, 46, 373
Tru1I	TTAA	3 cut(s)	686, 831, 945
Tru9I	TTAA	3 cut(s)	686, 831, 945
TscAI	CASTG	2 cut(s)	111, 1261
TseI	GCWGC	2 cut(s)	419, 1415
TspDTI	ATGAA	6 cut(s)	6, 650, 815, 984, 1247, 1284
TspGWI	ACGGA	1 cut(s)	854
TspRI	CASTG	2 cut(s)	111, 1261
VpaK11BI	GGWCC	2 cut(s)	74, 1021
VspI	ATTAAT	1 cut(s)	945
XapI	RAATTY	3 cut(s)	192, 1177, 1394
XceI	RCATGY	2 cut(s)	269, 1277
XmaJI	CCTAGG	1 cut(s)	509
ZrmI	AGTACT	1 cut(s)	478
Zsp2I	ATGCAT	1 cut(s)	933

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.