Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0004508

Physical Location & Seq

Genomic Coordinates Forward (+)

9384461 .. 9396963

Gene Structure 12503 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ22813

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2445 bp

ATGGCAATCGACATTGCAACCTTAGAAGAGAGGTACATTAATTCCTGCAGGAAGCACTGTGTTTCACCTAATGATGCAATTCTCTCTGTTCTGTTTAAGGCTGAGGTAAAAAAGTCTTGCAATGAGCTCTGTAGCCTGGAGATTTCATTGGACCATCTTAAGGATGCTGAATTCCCACCACTTCTTGATTTATGTATGGAACTTGATGCTTCTGAGGTTGAAGCAGTTGACATACGCAATGAATCATTGCATGTTTTGAATGGAAAATATGCGCTGTTGTTGATGCGTGCTATCAATCAAAAGCTTCGAGTTGTTGATCTCCAAGATTTGGCACTTGGAAAGGAGTTCTTGAGGGATCTTTCACAGAGAGGTTTGACATGCCAAGTTTTAAACTTGAGATCTTCACATTTTCGGAAGCTCAATATGATGGGAGAGTTCATGCGGATACACACCCTCAACCTTGACTTTAGTACTTCACTCACTAGTTTTCTGGAGGATTGTTTTTCTTGCATGCCCAATCTAATGTGCCTCTCTCTGTGCGAGACAAGAATTTTAAATCTCTGGACAACTATTGCTGCGCTTTCTAAACTCCCTTCTTTGGTTGAACTTCGGTTTCAGCATTGGTTATGCTGCAATGATGTTGGGCCTTATTCTGCATCATCTAGTGGGAAGTCACATCACAAAACTTATTCAAATCAGCGAAATAATGGTAGAACATCATCCATTAATATTGGGGAACTAACAGACCAGTACTCAAGCACAGAAGAGGTGCTCAGGAATATGTTTTTGCTTAATAATGTGGTTATAAATGATGATGAAAGTAGGGCTGAGGATTCAGATGATAGCGAACTAGACTTTTCAAGTCCCCTGCAAGAACATGGTTCCGTGGAGCATTTGTCAAATGTCTTTTCAAGAGGGAACAGACAGATCAATCAACAGACTGAGGATTCTCTTGATAACTTTCAGAATCAAAATGAGGCAGAGCCCTTAGCTGGTCCCTTCACAAAGTATATTGGAGACAATTCAGTGAAATATATTTCTTATCATGCTTCGCCAATATGTTTTGAGAAATATTATAGAGAGTACATGATAGCCTCACTACCTCGTTTGAGAATTTTAGATAACTTGCCAATTCGAAAGATTGATCAGGAAACTGCTAGGATGACATTTTCAGAATATTTTGAGCATGTACCATATCAAAGAAAGCATAAAGAAAACGTCATTAGTGTGTTACAAAAGCGTGAAATAAGATCAAGTCAAAATCATGTACAAAGTCCTGGGAAAAAAGTATCGAATCTCTATGGAAAGTCTCAATGCTTCTATACCAGGTCTCTTTCTGCTGCCAAGCTGGGATCTTGTGCTTGGCCATTCTTGAACCCGCTTTCTTTTTCAAGCAGTATTCTGGTAGGTGAAAGTAAGAGCTTTCGTCCTAGGCAGTTTGAGTATCATCCATCCAACTCCAGCCATATGGTCTTTGGAACATTAGATGGTGAAATTGTTGTAGTCAACCATGAGAATGGGAAAATTGTCAGTTATATCCCATCACTAGGAGCAATGAATAGTGTGCTGGGACTCTGTTGGCTCAAGAAGTATCCCTCTAAGCTAATAGCTGGTTCAGATAATGGCTCATTGAAATTGTACGATATCCATCATATGCGATCAATGGAAACAGGCATATGTGGTGGTGCCGAGTCTGTCTCATTTGACGAGTTTGACCAGTTGACATCTGTTCATGTTAATTCCACGGACGAGCTATTTCTTGCTAGTGGATACTCGAGAGACGTTGCCTTGTATGACATTAGTAGTGGAAAACGCTTACAGGTGTTTACTAATATGCATCGGGAGCATATTAATGTAATAAAGTTTGCAAACCATTCCCCTTCTGTTTTCGCCACTTCTTCATTCGATCAGGATGTCAAGATGTGGGACCTAAGACAGAAACCAATAAGTCCTTGCTATTCCTCTTCAAGCTCTAGGGGTAATGTGATGGTTTGCTTTTCTCCTGATGATCACTATCTTCTTGTCTCAGCTGTTGATAACGAGGTTAGGCAACTGCTCGCAGTTGATGGGAGACTTCACTTGAATTTTGAGATAGCCTCTACAGGAAGTTCTCAGAACTACACTCGATCCTATTACATGAATGGAAGAGATTATATCATCAGTGGGAGTTGTGATGAGCATGTTGTCCGCATATGCTGTGCTCAAACAGGGAGACGCCTGAGGGATATATCTTTGGAGGGAGGAGGAAGATCTAGGAGTTCTATGTTTGTGCAATCTTTGAGGGGTGATCCTTTCAGAGAATTCAACATGAGTATCTTGGCAGCCCATATGCGTCCAAGATCGAAGTCTGAAATTATCAAGGTCAATCTGCTAGCATCTAGAGATTATGCCAAGGAACACTTTGATGCTCAGCAGGCTTGCCCAACCAATAATATGGGAGGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000209 GO:0000715 GO:0003674 GO:0003676 GO:0003677 GO:0003684 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0006139 GO:0006259 GO:0006281 GO:0006289 GO:0006293 GO:0006294 GO:0006296 GO:0006325 GO:0006464 GO:0006508 GO:0006513 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008150 GO:0008152 GO:0009314 GO:0009411 GO:0009416 GO:0009628 GO:0009987 GO:0010390 GO:0016043 GO:0016567 GO:0016569 GO:0016570 GO:0016574 GO:0016579 GO:0019538 GO:0022607 GO:0030054 GO:0031334 GO:0031461 GO:0031465 GO:0031974 GO:0031981 GO:0032446 GO:0032991 GO:0033522 GO:0033554 GO:0033683 GO:0034622 GO:0034641 GO:0034644 GO:0035518 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043254 GO:0043412 GO:0043687 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044877 GO:0046483 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0050896 GO:0051128 GO:0051130 GO:0051276 GO:0051716 GO:0051865 GO:0065003 GO:0065004 GO:0065007 GO:0070013 GO:0070646 GO:0070647 GO:0070911 GO:0070914 GO:0071214 GO:0071478 GO:0071482 GO:0071704 GO:0071824 GO:0071840 GO:0080008 GO:0090304 GO:0090305 GO:0097159 GO:0104004 GO:1901360 GO:1901363 GO:1901564 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

WD40

Protein Analysis

814

Amino Acids

92.07

Weight (kDa)

6.35

Isoelectric Point (pI)

47.86

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DHU1_N	PF20919	4 - 391	2.3e-87	DWD hypersensitive to UV-B 1, N-terminal
WD40_Prp19	PF24814	480 - 683	3e-11	Prp19 WD40 domain
Beta-prop_WDR36-Utp21_1st	PF25171	488 - 685	1.6e-13	WDR36/Utp21 first beta-propeller
Beta-prop_WDR5	PF25175	490 - 729	9.5e-18	WDR5 beta-propeller domain
Beta-prop_RIG_2nd	PF23775	523 - 672	3.1e-06	RIG second beta-propeller
WD40_CDC20-Fz	PF24807	570 - 683	1.7e-06	CDC20/Fizzy WD40 domain
Beta-prop_THOC3	PF25174	574 - 725	4e-11	THOC3 beta-propeller domain
Beta-prop_WDR3_1st	PF25173	574 - 683	5e-08	WDR3 first beta-propeller domain
WD40_WDHD1_1st	PF24817	574 - 729	8e-06	WDHD1 first WD40 domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004508)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34280
fragaria_vesca	FvH4_2g04670 FvH4_2g04670
malus_domestica	MD05G1093800.v1.1
prunus_persica	Prupe.8G138700_v2.0.a1 Prupe.8G138700_v2.0.a1
pyrus_communis	pycom05g09290
rosa_chinensis	RchiOBHm_Chr6g0254291 RchiOBHm_Chr6g0254331 RchiOBHm_Chr6g0254371
rosa_laevigata	RLG00000014132 RLG00000014922 RLG00000014929
rosa_multiflora	Rmu_sc0012713.1_g000001 Rmu_sc0012713.1_g000002 Rmu_sc0027609.1_g000001 Rmu_ssc0000412.1_g000017
rosa_roxburghii	Rroxscaffold_7G00210950
rosa_rugosa	Rorug05G0551700 Rorug05G0551800 Rorug05G0551800
rosa_samantha	Rh6AG068100 Rh6AG068600 Rh6BG061000 Rh6BG061300 Rh6CG060800 Rh6CG061200 Rh6DG057900 Rh6DG058100 Rh6DG058500
rosa_wichuraiana	Rw6G005970 Rw6G006010

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	806
AccB1I	GGYRCC	1 cut(s)	1685
AccB7I	CCANNNNNTGG	1 cut(s)	328
AciI	CCGC	3 cut(s)	442, 1379, 2188
AclWI	GGATC	4 cut(s)	363, 1360, 2121, 2282
AcoI	YGGCCR	1 cut(s)	1364
AcsI	RAATTY	5 cut(s)	170, 549, 1113, 2083, 2300
AcyI	GRCGYC	1 cut(s)	2215
AfaI	GTAC	7 cut(s)	35, 472, 752, 1085, 1191, 1269, 1640
AfiI	CCNNNNNNNGG	5 cut(s)	160, 328, 598, 992, 1547
AflII	CTTAAG	1 cut(s)	158
AhlI	ACTAGT	1 cut(s)	482
AjnI	CCWGG	3 cut(s)	135, 1276, 1325
Alw21I	GWGCWC	3 cut(s)	129, 774, 2203
Alw26I	GTCTC	9 cut(s)	536, 1011, 1314, 1335, 1702, 1773, 2029, 2065, 2206
AlwI	GGATC	4 cut(s)	363, 1360, 2121, 2282
Ama87I	CYCGRG	1 cut(s)	1774
AoxI	GGCC	2 cut(s)	644, 1364
ApeKI	GCWGC	4 cut(s)	575, 630, 1340, 2321
ApoI	RAATTY	5 cut(s)	170, 549, 1113, 2083, 2300
AseI	ATTAAT	3 cut(s)	39, 726, 1851
AspA2I	CCTAGG	1 cut(s)	1430
AspLEI	GCGC	2 cut(s)	274, 580
AspS9I	GGNCC	4 cut(s)	151, 644, 995, 1927
AsuHPI	GGTGA	4 cut(s)	57, 1421, 1502, 2297
AsuII	TTCGAA	1 cut(s)	1135
AsuNHI	GCTAGC	1 cut(s)	2371
AvaI	CYCGRG	1 cut(s)	1774
AvaII	GGWCC	3 cut(s)	151, 995, 1927
AvrII	CCTAGG	1 cut(s)	1430
AxyI	CCTNAGG	1 cut(s)	2219
BaeI	ACNNNNGTAYC	2 cut(s)	2296, 2329
BalI	TGGCCA	1 cut(s)	1366
BanI	GGYRCC	1 cut(s)	1685
BanII	GRGCYC	2 cut(s)	129, 987
Bbv12I	GWGCWC	3 cut(s)	129, 774, 2203
BbvCI	CCTCAGC	2 cut(s)	102, 828
BbvI	GCAGC	4 cut(s)	562, 617, 1327, 2333
BccI	CCATC	8 cut(s)	162, 421, 1459, 1481, 1549, 1656, 1981, 2060
BciT130I	CCWGG	3 cut(s)	137, 1278, 1327
BciVI	GTATCC	3 cut(s)	438, 1602, 1763
BclI	TGATCA	2 cut(s)	1144, 2008
BcoDI	GTCTC	9 cut(s)	536, 1011, 1314, 1335, 1702, 1773, 2029, 2065, 2206
BcuI	ACTAGT	1 cut(s)	482
BfmI	CTRYAG	3 cut(s)	46, 130, 2100
BfrI	CTTAAG	1 cut(s)	158
BfuI	GTATCC	3 cut(s)	438, 1602, 1763
BglII	AGATCT	2 cut(s)	398, 2249
BisI	GCNGC	4 cut(s)	576, 631, 1341, 2322
BlnI	CCTAGG	1 cut(s)	1430
BlpI	GCTNAGC	1 cut(s)	2409
BlsI	GCNGC	4 cut(s)	577, 632, 1342, 2323
BmcAI	AGTACT	2 cut(s)	472, 752
Bme1390I	CCNGG	3 cut(s)	137, 1278, 1327
Bme18I	GGWCC	3 cut(s)	151, 995, 1927
BmeT110I	CYCGRG	1 cut(s)	1774
BmgT120I	GGNCC	4 cut(s)	151, 644, 995, 1927
BmiI	GGNNCC	4 cut(s)	883, 997, 1687, 1928
BmrFI	CCNGG	3 cut(s)	137, 1278, 1327
BmsI	GCATC	8 cut(s)	64, 154, 196, 273, 665, 1846, 2384, 2395
BmtI	GCTAGC	1 cut(s)	2375
BplI	GAGNNNNNCTC	4 cut(s)	1682, 1714, 2081, 2113
BpmI	CTGGAG	3 cut(s)	158, 512, 1444
Bpu10I	CCTNAGC	4 cut(s)	102, 773, 828, 988
Bpu1102I	GCTNAGC	1 cut(s)	2409
Bpu14I	TTCGAA	1 cut(s)	1135
BpuEI	CTTGAG	4 cut(s)	370, 415, 739, 1568
BsaBI	GATNNNNATC	2 cut(s)	1647, 2013
BsaHI	GRCGYC	1 cut(s)	2215
BsaI	GGTCTC	1 cut(s)	1335
BsaJI	CCNNGG	5 cut(s)	885, 1277, 1430, 1743, 2391
BsaXI	ACNNNNNCTCC	2 cut(s)	881, 911
Bsc4I	CCNNNNNNNGG	5 cut(s)	160, 328, 598, 992, 1547
Bse1I	ACTGG	2 cut(s)	748, 1717
Bse21I	CCTNAGG	1 cut(s)	2219
Bse3DI	GCAATG	6 cut(s)	12, 127, 244, 245, 640, 1560
Bse8I	GATNNNNATC	2 cut(s)	1647, 2013
BseBI	CCWGG	3 cut(s)	137, 1278, 1327
BseDI	CCNNGG	5 cut(s)	885, 1277, 1430, 1743, 2391
BseGI	GGATG	6 cut(s)	169, 719, 1167, 1447, 1451, 1918
BseJI	GATNNNNATC	2 cut(s)	1647, 2013
BseLI	CCNNNNNNNGG	5 cut(s)	160, 328, 598, 992, 1547
BseMI	GCAATG	6 cut(s)	12, 127, 244, 245, 640, 1560
BseMII	CTCAG	9 cut(s)	93, 204, 787, 819, 933, 2040, 2126, 2210, 2423
BseNI	ACTGG	2 cut(s)	748, 1717
BseRI	GAGGAG	1 cut(s)	2256
BseXI	GCAGC	4 cut(s)	562, 617, 1327, 2333
BseYI	CCCAGC	2 cut(s)	1348, 1567
BshFI	GGCC	2 cut(s)	646, 1366
BshNI	GGYRCC	1 cut(s)	1685
BsiHKAI	GWGCWC	3 cut(s)	129, 774, 2203
BsiHKCI	CYCGRG	1 cut(s)	1774
BslFI	GGGAC	4 cut(s)	849, 981, 1584, 1940
BslI	CCNNNNNNNGG	5 cut(s)	160, 328, 598, 992, 1547
BsmAI	GTCTC	9 cut(s)	536, 1011, 1314, 1335, 1702, 1773, 2029, 2065, 2206
BsmBI	CGTCTC	2 cut(s)	1773, 2206
BsmFI	GGGAC	4 cut(s)	849, 981, 1584, 1940
BsnI	GGCC	2 cut(s)	646, 1366
Bso31I	GGTCTC	1 cut(s)	1335
BsoBI	CYCGRG	1 cut(s)	1774
Bsp119I	TTCGAA	1 cut(s)	1135
Bsp1286I	GDGCHC	4 cut(s)	129, 774, 987, 2203
Bsp1407I	TGTACA	1 cut(s)	1267
Bsp1720I	GCTNAGC	1 cut(s)	2409
BspACI	CCGC	3 cut(s)	442, 1379, 2188
BspANI	GGCC	2 cut(s)	646, 1366
BspCNI	CTCAG	9 cut(s)	94, 205, 786, 820, 934, 2039, 2125, 2211, 2422
BspLI	GGNNCC	4 cut(s)	883, 997, 1687, 1928
BspMAI	CTGCAG	1 cut(s)	50
BspOI	GCTAGC	1 cut(s)	2375
BspPI	GGATC	4 cut(s)	363, 1360, 2121, 2282
BspT104I	TTCGAA	1 cut(s)	1135
BspT107I	GGYRCC	1 cut(s)	1685
BspTI	CTTAAG	1 cut(s)	158
BspTNI	GGTCTC	1 cut(s)	1335
BsrDI	GCAATG	6 cut(s)	12, 127, 244, 245, 640, 1560
BsrGI	TGTACA	1 cut(s)	1267
BsrI	ACTGG	2 cut(s)	748, 1717
BssECI	CCNNGG	5 cut(s)	885, 1277, 1430, 1743, 2391
BssNI	GRCGYC	1 cut(s)	2215
BssT1I	CCWWGG	2 cut(s)	1430, 2391
Bst2UI	CCWGG	3 cut(s)	137, 1278, 1327
Bst4CI	ACNGT	1 cut(s)	59
Bst6I	CTCTTC	4 cut(s)	21, 759, 1969, 2140
BstACI	GRCGYC	1 cut(s)	2215
BstAFI	CTTAAG	1 cut(s)	158
BstAUI	TGTACA	1 cut(s)	1267
BstBI	TTCGAA	1 cut(s)	1135
BstC8I	GCNNGC	6 cut(s)	288, 512, 2058, 2373, 2415, 2419
BstDSI	CCRYGG	2 cut(s)	885, 1743
BstF5I	GGATG	6 cut(s)	169, 719, 1167, 1447, 1451, 1918
BstHHI	GCGC	2 cut(s)	274, 580
BstMAI	GTCTC	9 cut(s)	536, 1011, 1314, 1335, 1702, 1773, 2029, 2065, 2206
BstMWI	GCNNNNNNNGC	2 cut(s)	1843, 2414
BstNI	CCWGG	3 cut(s)	137, 1278, 1327
BstNSI	RCATGY	5 cut(s)	254, 381, 514, 1190, 2183
BstSCI	CCNGG	3 cut(s)	135, 1276, 1325
BstSFI	CTRYAG	3 cut(s)	46, 130, 2100
BstV1I	GCAGC	4 cut(s)	562, 617, 1327, 2333
BstX2I	RGATCY	4 cut(s)	355, 398, 1352, 2249
BstXI	CCANNNNNNTGG	3 cut(s)	1468, 1517, 2434
BstYI	RGATCY	4 cut(s)	355, 398, 1352, 2249
Bsu36I	CCTNAGG	1 cut(s)	2219
BsuI	GTATCC	3 cut(s)	438, 1602, 1763
BsuRI	GGCC	2 cut(s)	646, 1366
BtgI	CCRYGG	2 cut(s)	885, 1743
BtsCI	GGATG	6 cut(s)	169, 719, 1167, 1447, 1451, 1918
BtsIMutI	CAGTG	3 cut(s)	55, 1032, 2167
Cac8I	GCNNGC	6 cut(s)	288, 512, 2058, 2373, 2415, 2419
CfoI	GCGC	2 cut(s)	274, 580
Cfr13I	GGNCC	4 cut(s)	151, 644, 995, 1927
CseI	GACGC	2 cut(s)	2223, 2321
CsiI	ACCWGGT	1 cut(s)	1325
Csp6I	GTAC	7 cut(s)	34, 471, 751, 1084, 1190, 1268, 1639
CviQI	GTAC	7 cut(s)	34, 471, 751, 1084, 1190, 1268, 1639
DraI	TTTAAA	2 cut(s)	390, 555
EaeI	YGGCCR	1 cut(s)	1364
Eam1104I	CTCTTC	4 cut(s)	21, 759, 1969, 2140
EarI	CTCTTC	4 cut(s)	21, 759, 1969, 2140
Ecl136II	GAGCTC	1 cut(s)	127
Eco130I	CCWWGG	2 cut(s)	1430, 2391
Eco24I	GRGCYC	2 cut(s)	129, 987
Eco31I	GGTCTC	1 cut(s)	1335
Eco32I	GATATC	1 cut(s)	1645
Eco47I	GGWCC	3 cut(s)	151, 995, 1927
Eco53kI	GAGCTC	1 cut(s)	127
Eco81I	CCTNAGG	1 cut(s)	2219
Eco88I	CYCGRG	1 cut(s)	1774
EcoICRI	GAGCTC	1 cut(s)	127
EcoO109I	RGGNCCY	1 cut(s)	1927
EcoRI	GAATTC	2 cut(s)	170, 2300
EcoRII	CCWGG	3 cut(s)	135, 1276, 1325
EcoRV	GATATC	1 cut(s)	1645
EcoT14I	CCWWGG	2 cut(s)	1430, 2391
EcoT22I	ATGCAT	1 cut(s)	1839
EcoT38I	GRGCYC	2 cut(s)	129, 987
ErhI	CCWWGG	2 cut(s)	1430, 2391
Esp3I	CGTCTC	2 cut(s)	1773, 2206
FalI	AAGNNNNNCTT	4 cut(s)	332, 364, 2384, 2416
FaqI	GGGAC	4 cut(s)	849, 981, 1584, 1940
FauI	CCCGC	1 cut(s)	1386
FauNDI	CATATG	5 cut(s)	1467, 1653, 1676, 2192, 2328
FbaI	TGATCA	2 cut(s)	1144, 2008
Fnu4HI	GCNGC	4 cut(s)	576, 631, 1341, 2322
FokI	GGATG	6 cut(s)	176, 706, 1174, 1434, 1438, 1925
FriOI	GRGCYC	2 cut(s)	129, 987
Fsp4HI	GCNGC	4 cut(s)	576, 631, 1341, 2322
GlaI	GCGC	2 cut(s)	273, 579
GluI	GCNGC	4 cut(s)	576, 631, 1341, 2322
GsaI	CCCAGC	2 cut(s)	1352, 1571
GsuI	CTGGAG	3 cut(s)	158, 512, 1444
HaeIII	GGCC	2 cut(s)	646, 1366
HgaI	GACGC	2 cut(s)	2223, 2321
HhaI	GCGC	2 cut(s)	274, 580
Hin1I	GRCGYC	1 cut(s)	2215
Hin6I	GCGC	2 cut(s)	272, 578
HinP1I	GCGC	2 cut(s)	272, 578
HincII	GTYRAC	3 cut(s)	229, 1507, 1722
HindII	GTYRAC	3 cut(s)	229, 1507, 1722
HindIII	AAGCTT	1 cut(s)	302
HinfI	GANTC	7 cut(s)	242, 833, 947, 967, 1294, 1572, 1691
HphI	GGTGA	4 cut(s)	57, 1421, 1502, 2297
Hpy166II	GTNNAC	4 cut(s)	229, 1507, 1722, 1827
Hpy188I	TCNGA	9 cut(s)	214, 414, 838, 966, 1174, 1618, 2115, 2297, 2350
Hpy8I	GTNNAC	4 cut(s)	229, 1507, 1722, 1827
HpyAV	CCTTC	3 cut(s)	603, 1009, 1890
HpyCH4III	ACNGT	1 cut(s)	59
HpyCH4IV	ACGT	2 cut(s)	1218, 1782
HpyF10VI	GCNNNNNNNGC	2 cut(s)	1843, 2414
HpySE526I	ACGT	2 cut(s)	1218, 1782
Hsp92I	GRCGYC	1 cut(s)	2215
HspAI	GCGC	2 cut(s)	272, 578
Ksp22I	TGATCA	2 cut(s)	1144, 2008
LmnI	GCTCC	3 cut(s)	889, 1550, 1843
Lsp1109I	GCAGC	4 cut(s)	562, 617, 1327, 2333
LweI	GCATC	8 cut(s)	64, 154, 196, 273, 665, 1846, 2384, 2395
MabI	ACCWGGT	1 cut(s)	1325
MaeII	ACGT	2 cut(s)	1218, 1782
MaeIII	GTNAC	2 cut(s)	672, 1230
MboII	GAAGA	8 cut(s)	38, 393, 776, 1890, 1956, 2009, 2157, 2259
MflI	RGATCY	4 cut(s)	355, 398, 1352, 2249
MhlI	GDGCHC	4 cut(s)	129, 774, 987, 2203
MlsI	TGGCCA	1 cut(s)	1366
MluNI	TGGCCA	1 cut(s)	1366
MlyI	GAGTC	2 cut(s)	1566, 1700
MmeI	TCCRAC	1 cut(s)	1479
Mox20I	TGGCCA	1 cut(s)	1366
Mph1103I	ATGCAT	1 cut(s)	1839
MscI	TGGCCA	1 cut(s)	1366
MseI	TTAA	9 cut(s)	39, 96, 159, 389, 554, 726, 792, 1737, 1851
MslI	CAYNNNNRTG	4 cut(s)	1226, 1515, 1851, 2403
Msp20I	TGGCCA	1 cut(s)	1366
MspA1I	CMGCKG	1 cut(s)	2030
MspCI	CTTAAG	1 cut(s)	158
MspR9I	CCNGG	3 cut(s)	137, 1278, 1327
MvaI	CCWGG	3 cut(s)	137, 1278, 1327
MwoI	GCNNNNNNNGC	2 cut(s)	1843, 2414
NdeI	CATATG	5 cut(s)	1467, 1653, 1676, 2192, 2328
NheI	GCTAGC	1 cut(s)	2371
NlaIV	GGNNCC	4 cut(s)	883, 997, 1687, 1928
NmeAIII	GCCGAG	1 cut(s)	1714
NmuCI	GTSAC	1 cut(s)	672
NsiI	ATGCAT	1 cut(s)	1839
NspI	RCATGY	5 cut(s)	254, 381, 514, 1190, 2183
NspV	TTCGAA	1 cut(s)	1135
PaeI	GCATGC	1 cut(s)	514
PaeR7I	CTCGAG	1 cut(s)	1774
PfeI	GAWTC	5 cut(s)	242, 833, 947, 967, 1294
PflMI	CCANNNNNTGG	1 cut(s)	328
PkrI	GCNGC	4 cut(s)	577, 632, 1342, 2323
PleI	GAGTC	2 cut(s)	1566, 1699
PpsI	GAGTC	2 cut(s)	1566, 1699
PpuMI	RGGWCCY	1 cut(s)	1927
PshBI	ATTAAT	3 cut(s)	39, 726, 1851
PsiI	TTATAA	1 cut(s)	806
Psp124BI	GAGCTC	1 cut(s)	129
Psp5II	RGGWCCY	1 cut(s)	1927
Psp6I	CCWGG	3 cut(s)	135, 1276, 1325
PspFI	CCCAGC	2 cut(s)	1348, 1567
PspGI	CCWGG	3 cut(s)	135, 1276, 1325
PspN4I	GGNNCC	4 cut(s)	883, 997, 1687, 1928
PspPI	GGNCC	4 cut(s)	151, 644, 995, 1927
PspPPI	RGGWCCY	1 cut(s)	1927
PstI	CTGCAG	1 cut(s)	50
PsuI	RGATCY	4 cut(s)	355, 398, 1352, 2249
PvuII	CAGCTG	1 cut(s)	2030
RsaI	GTAC	7 cut(s)	35, 472, 752, 1085, 1191, 1269, 1640
RsaNI	GTAC	7 cut(s)	34, 471, 751, 1084, 1190, 1268, 1639
RseI	CAYNNNNRTG	4 cut(s)	1226, 1515, 1851, 2403
SacI	GAGCTC	1 cut(s)	129
SaqAI	TTAA	9 cut(s)	39, 96, 159, 389, 554, 726, 792, 1737, 1851
SatI	GCNGC	4 cut(s)	576, 631, 1341, 2322
Sau96I	GGNCC	4 cut(s)	151, 644, 995, 1927
SbfI	CCTGCAGG	1 cut(s)	50
ScaI	AGTACT	2 cut(s)	472, 752
SchI	GAGTC	2 cut(s)	1566, 1700
ScrFI	CCNGG	3 cut(s)	137, 1278, 1327
SdaI	CCTGCAGG	1 cut(s)	50
SduI	GDGCHC	4 cut(s)	129, 774, 987, 2203
SexAI	ACCWGGT	1 cut(s)	1325
SfaNI	GCATC	8 cut(s)	64, 154, 196, 273, 665, 1846, 2384, 2395
SfcI	CTRYAG	3 cut(s)	46, 130, 2100
Sfr274I	CTCGAG	1 cut(s)	1774
SfuI	TTCGAA	1 cut(s)	1135
SinI	GGWCC	3 cut(s)	151, 995, 1927
SlaI	CTCGAG	1 cut(s)	1774
SmiMI	CAYNNNNRTG	4 cut(s)	1226, 1515, 1851, 2403
SmlI	CTYRAG	6 cut(s)	158, 349, 394, 754, 1583, 1774
SmoI	CTYRAG	6 cut(s)	158, 349, 394, 754, 1583, 1774
SpeI	ACTAGT	1 cut(s)	482
SphI	GCATGC	1 cut(s)	514
Sse8387I	CCTGCAGG	1 cut(s)	50
SsiI	CCGC	3 cut(s)	442, 1379, 2188
SspI	AATATT	3 cut(s)	730, 1073, 1178
SstI	GAGCTC	1 cut(s)	129
StyD4I	CCNGG	3 cut(s)	135, 1276, 1325
StyI	CCWWGG	2 cut(s)	1430, 2391
TaaI	ACNGT	1 cut(s)	59
TaiI	ACGT	2 cut(s)	1221, 1785
TaqI	TCGA	8 cut(s)	9, 307, 1135, 1292, 1775, 1905, 2125, 2342
TatI	WGTACW	4 cut(s)	470, 750, 1083, 1267
TfiI	GAWTC	5 cut(s)	242, 833, 947, 967, 1294
Tru1I	TTAA	9 cut(s)	39, 96, 159, 389, 554, 726, 792, 1737, 1851
Tru9I	TTAA	9 cut(s)	39, 96, 159, 389, 554, 726, 792, 1737, 1851
TscAI	CASTG	3 cut(s)	62, 1032, 2167
TseFI	GTSAC	1 cut(s)	672
TseI	GCWGC	4 cut(s)	575, 630, 1340, 2321
Tsp45I	GTSAC	1 cut(s)	672
TspDTI	ATGAA	8 cut(s)	135, 255, 427, 831, 1571, 1721, 1890, 2153
TspGWI	ACGGA	2 cut(s)	874, 1760
TspRI	CASTG	3 cut(s)	62, 1032, 2167
Van91I	CCANNNNNTGG	1 cut(s)	328
Vha464I	CTTAAG	1 cut(s)	158
VpaK11BI	GGWCC	3 cut(s)	151, 995, 1927
VspI	ATTAAT	3 cut(s)	39, 726, 1851
XapI	RAATTY	5 cut(s)	170, 549, 1113, 2083, 2300
XbaI	TCTAGA	1 cut(s)	2378
XceI	RCATGY	5 cut(s)	254, 381, 514, 1190, 2183
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1472
XhoI	CTCGAG	1 cut(s)	1774
XmaJI	CCTAGG	1 cut(s)	1430
ZrmI	AGTACT	2 cut(s)	472, 752
Zsp2I	ATGCAT	1 cut(s)	1839

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.