MD05G1114400.v1.1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009469
Physical Location & Seq
Forward (+)
23313036 .. 23316643
3608 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1114400.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGGAGAAAAAAAGTGAGAGTGGGAGTGGTGGTGGACAGGTGGTGGACGGTTCGAATATAATGGAGTTGGTTGGGAACGAGGAGGTGTTTAGTAACTTTGTGGAGCATAAGTTTAAGGAGCTCGACAGGAACAGAGACGGCCACCTCTCTTTGAACGAGCTCCACCCTGCTGTCGCCGATATTGGCCTTGCCCTTGGCTTGCCGGCTCAGGGTTCTTCCCGGGACTCCGATCACATCTATTCCGAGGTATTGAATGAGTTCACTCATGGGAAACAACAAAAGGTGAGCAAGACTGAGTTCAAAGAGGTTCTTTCGGATATTCTAATAGGCATGGCTGCTGGTCTCAAGCGAGACCCTATTGTGATCCTCCGTATTGATGGAGAAGACCTCCTTGAATTCATCGATGGGCCAATTTTTGAACCAGAGCTGGTGGCTACATATTCTGAGGTAAAGTCAGCTGATGGAACTCTCCGTGACGGTATAATCAAAGCTTTGGAAAAACTCACAGTTGACCAAGGGATGCCCCCTTCATCGGATTCTTGGGTTGTAAGCAATATTGTGGAACCTGCTCTGCAATCATGCACTGGCCATGATTTGGACAAGCCGGTCTCTCAAGATACGTTCCTAGAAGAATTCAAAAAAGTGGCAGAGTTTGTGGCTCATCGTCTTCAGGAGCAGCCTGTGATTGTAGCCCACAGTGAGAATGACTTTGATGGAAGTGACATCAAGAGATTGTTGTCCAACAAGTTTGAATTAGACAAGACACTGAATGCAGCTATTGAAACTGTTTCAAAAGACCGCAGTGGGAAATTATCCAAGGAATATTTGCGTGTGGCACTGGATGCGGTGGCTCCAACAGCTGGTCTGCCACCACTTGGTGCAGTTGAGCAGATGGACAAGGTTGTGCAGGATGTGTTCAACATGCTTAACGCAGATGACGGAAAGCAGCTTAAAGAAGACGAGTTCAAGAAGACACTGACTGAAATCCTGGGGAGTATCATGTTGCAATTGGAGGGAAACCCAATCTCAGTTCACTCTAATGCGGTTGTGCACGAGCCCCTCACCGCTGCTTCTACTCTACTGCAGCCAAGTCCCGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

368

Amino Acids

40.09

Weight (kDa)

4.7

Isoelectric Point (pI)

45.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 36 - 104 3.5e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 605
Acc36I ACCTGC 1 cut(s) 574
AccB7I CCANNNNNTGG 3 cut(s) 595, 860, 875
AciI CCGC 4 cut(s) 799, 845, 1043, 1065
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 2 cut(s) 139, 586
AcsI RAATTY 2 cut(s) 395, 632
AcuI CTGAAG 1 cut(s) 653
AdeI CACNNNGTG 1 cut(s) 878
AfiI CCNNNNNNNGG 5 cut(s) 209, 532, 595, 860, 875
AjnI CCWGG 1 cut(s) 987
AluBI AGCT 8 cut(s) 121, 160, 427, 458, 491, 776, 860, 949
AluI AGCT 8 cut(s) 121, 160, 427, 458, 491, 776, 860, 949
Alw21I GWGCWC 3 cut(s) 123, 162, 1053
Alw26I GTCTC 4 cut(s) 129, 345, 347, 613
Alw44I GTGCAC 1 cut(s) 1049
AlwI GGATC 1 cut(s) 358
Ama87I CYCGRG 2 cut(s) 219, 1094
AoxI GGCC 4 cut(s) 139, 184, 407, 586
ApaLI GTGCAC 1 cut(s) 1049
ApeKI GCWGC 6 cut(s) 335, 676, 773, 946, 1067, 1084
ApoI RAATTY 2 cut(s) 395, 632
Asp700I GAANNNNTTC 1 cut(s) 257
AspS9I GGNCC 1 cut(s) 407
AsuC2I CCSGG 2 cut(s) 220, 221
AsuHPI GGTGA 2 cut(s) 295, 1054
AsuII TTCGAA 1 cut(s) 53
AvaI CYCGRG 2 cut(s) 219, 1094
BaeGI GKGCMC 1 cut(s) 1053
BalI TGGCCA 1 cut(s) 588
BanII GRGCYC 3 cut(s) 123, 162, 1059
BauI CACGAG 1 cut(s) 1052
BbsI GAAGAC 4 cut(s) 390, 659, 963, 977
Bbv12I GWGCWC 3 cut(s) 123, 162, 1053
BbvI GCAGC 6 cut(s) 322, 688, 785, 958, 1054, 1096
BccI CCATC 5 cut(s) 371, 398, 455, 707, 886
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 1 cut(s) 989
BcnI CCSGG 2 cut(s) 220, 221
BcoDI GTCTC 4 cut(s) 129, 345, 347, 613
BfaI CTAG 1 cut(s) 626
BfmI CTRYAG 1 cut(s) 1082
BfuAI ACCTGC 1 cut(s) 574
BisI GCNGC 6 cut(s) 336, 677, 774, 947, 1068, 1085
BlsI GCNGC 6 cut(s) 337, 678, 775, 948, 1069, 1086
Bme1390I CCNGG 3 cut(s) 220, 221, 989
BmeT110I CYCGRG 2 cut(s) 219, 1094
BmgT120I GGNCC 1 cut(s) 407
BmiI GGNNCC 2 cut(s) 564, 852
BmrFI CCNGG 3 cut(s) 220, 221, 989
BmsI GCATC 2 cut(s) 510, 832
BpiI GAAGAC 4 cut(s) 390, 659, 963, 977
BplI GAGNNNNNCTC 2 cut(s) 372, 404
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 53
BpuEI CTTGAG 2 cut(s) 329, 597
BpuMI CCSGG 2 cut(s) 220, 221
Bsa29I ATCGAT 1 cut(s) 402
BsaI GGTCTC 3 cut(s) 345, 347, 613
BsaJI CCNNGG 6 cut(s) 193, 219, 243, 514, 816, 988
BsaXI ACNNNNNCTCC 4 cut(s) 16, 46, 95, 125
Bsc4I CCNNNNNNNGG 5 cut(s) 209, 532, 595, 860, 875
Bse118I RCCGGY 2 cut(s) 202, 604
Bse1I ACTGG 2 cut(s) 589, 843
BseBI CCWGG 1 cut(s) 989
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 6 cut(s) 193, 219, 243, 514, 816, 988
BseGI GGATG 3 cut(s) 525, 847, 916
BseLI CCNNNNNNNGG 5 cut(s) 209, 532, 595, 860, 875
BseMII CTCAG 4 cut(s) 221, 285, 435, 1041
BseNI ACTGG 2 cut(s) 589, 843
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 1053
BseXI GCAGC 6 cut(s) 322, 688, 785, 958, 1054, 1096
BsgI GTGCAG 2 cut(s) 900, 926
BshFI GGCC 4 cut(s) 141, 186, 409, 588
BshVI ATCGAT 1 cut(s) 402
BsiHKAI GWGCWC 3 cut(s) 123, 162, 1053
BsiHKCI CYCGRG 2 cut(s) 219, 1094
BsiSI CCGG 3 cut(s) 203, 220, 605
BslFI GGGAC 2 cut(s) 236, 1077
BslI CCNNNNNNNGG 5 cut(s) 209, 532, 595, 860, 875
BsmAI GTCTC 4 cut(s) 129, 345, 347, 613
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 236, 1077
BsmI GAATGC 1 cut(s) 775
BsnI GGCC 4 cut(s) 141, 186, 409, 588
Bso31I GGTCTC 3 cut(s) 345, 347, 613
BsoBI CYCGRG 2 cut(s) 219, 1094
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 4 cut(s) 123, 162, 1053, 1059
Bsp143I GATC 2 cut(s) 229, 363
BspACI CCGC 4 cut(s) 799, 845, 1043, 1065
BspANI GGCC 4 cut(s) 141, 186, 409, 588
BspCNI CTCAG 4 cut(s) 220, 286, 436, 1040
BspDI ATCGAT 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 564, 852
BspMAI CTGCAG 1 cut(s) 1086
BspMI ACCTGC 1 cut(s) 574
BspPI GGATC 1 cut(s) 358
BspT104I TTCGAA 1 cut(s) 53
BspTNI GGTCTC 3 cut(s) 345, 347, 613
BsrFI RCCGGY 2 cut(s) 202, 604
BsrI ACTGG 2 cut(s) 589, 843
BssAI RCCGGY 2 cut(s) 202, 604
BssECI CCNNGG 6 cut(s) 193, 219, 243, 514, 816, 988
BssMI GATC 2 cut(s) 229, 363
BssSI CACGAG 1 cut(s) 1052
BssT1I CCWWGG 3 cut(s) 193, 514, 816
Bst2BI CACGAG 1 cut(s) 1052
Bst2UI CCWGG 1 cut(s) 989
Bst4CI ACNGT 5 cut(s) 50, 479, 508, 698, 787
BstAPI GCANNNNNTGC 1 cut(s) 842
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 2 cut(s) 200, 204
BstDEI CTNAG 4 cut(s) 207, 294, 444, 1027
BstF5I GGATG 3 cut(s) 525, 847, 916
BstKTI GATC 2 cut(s) 232, 366
BstMAI GTCTC 4 cut(s) 129, 345, 347, 613
BstMBI GATC 2 cut(s) 229, 363
BstMWI GCNNNNNNNGC 2 cut(s) 842, 857
BstNI CCWGG 1 cut(s) 989
BstNSI RCATGY 1 cut(s) 925
BstSCI CCNGG 3 cut(s) 218, 219, 987
BstSFI CTRYAG 1 cut(s) 1082
BstSLI GKGCMC 1 cut(s) 1053
BstV1I GCAGC 6 cut(s) 322, 688, 785, 958, 1054, 1096
BstV2I GAAGAC 4 cut(s) 390, 659, 963, 977
Bsu15I ATCGAT 1 cut(s) 402
BsuRI GGCC 4 cut(s) 141, 186, 409, 588
BsuTUI ATCGAT 1 cut(s) 402
BtsCI GGATG 3 cut(s) 525, 847, 916
BtsI GCAGTG 1 cut(s) 808
BtsIMutI CAGTG 6 cut(s) 582, 703, 764, 808, 836, 974
BveI ACCTGC 1 cut(s) 574
Cac8I GCNNGC 2 cut(s) 200, 204
Cfr10I RCCGGY 2 cut(s) 202, 604
Cfr13I GGNCC 1 cut(s) 407
Cfr9I CCCGGG 1 cut(s) 219
ClaI ATCGAT 1 cut(s) 402
CviAII CATG 6 cut(s) 266, 331, 579, 590, 922, 1000
DdeI CTNAG 4 cut(s) 207, 294, 444, 1027
DpnI GATC 2 cut(s) 231, 365
DpnII GATC 2 cut(s) 229, 363
DraIII CACNNNGTG 1 cut(s) 878
DrdI GACNNNNNNGTC 1 cut(s) 605
DseDI GACNNNNNNGTC 1 cut(s) 605
EaeI YGGCCR 2 cut(s) 139, 586
Ecl136II GAGCTC 2 cut(s) 121, 160
Eco130I CCWWGG 3 cut(s) 193, 514, 816
Eco24I GRGCYC 3 cut(s) 123, 162, 1059
Eco31I GGTCTC 3 cut(s) 345, 347, 613
Eco53kI GAGCTC 2 cut(s) 121, 160
Eco57I CTGAAG 1 cut(s) 653
Eco88I CYCGRG 2 cut(s) 219, 1094
EcoICRI GAGCTC 2 cut(s) 121, 160
EcoRI GAATTC 2 cut(s) 395, 632
EcoRII CCWGG 1 cut(s) 987
EcoT14I CCWWGG 3 cut(s) 193, 514, 816
EcoT38I GRGCYC 3 cut(s) 123, 162, 1059
ErhI CCWWGG 3 cut(s) 193, 514, 816
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
FalI AAGNNNNNCTT 4 cut(s) 294, 326, 375, 407
FaqI GGGAC 2 cut(s) 236, 1077
FatI CATG 6 cut(s) 265, 330, 578, 589, 921, 999
Fnu4HI GCNGC 6 cut(s) 336, 677, 774, 947, 1068, 1085
FokI GGATG 3 cut(s) 532, 854, 923
FriOI GRGCYC 3 cut(s) 123, 162, 1059
Fsp4HI GCNGC 6 cut(s) 336, 677, 774, 947, 1068, 1085
FspBI CTAG 1 cut(s) 626
GluI GCNGC 6 cut(s) 336, 677, 774, 947, 1068, 1085
HaeIII GGCC 4 cut(s) 141, 186, 409, 588
HapII CCGG 3 cut(s) 203, 220, 605
Hin1II CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 2 cut(s) 224, 536
HpaII CCGG 3 cut(s) 203, 220, 605
HphI GGTGA 2 cut(s) 295, 1054
Hpy166II GTNNAC 6 cut(s) 35, 46, 261, 511, 1033, 1051
Hpy188I TCNGA 5 cut(s) 229, 244, 316, 445, 535
Hpy188III TCNNGA 5 cut(s) 614, 671, 727, 967, 1094
Hpy8I GTNNAC 6 cut(s) 35, 46, 261, 511, 1033, 1051
HpyAV CCTTC 1 cut(s) 537
HpyCH4III ACNGT 5 cut(s) 50, 479, 508, 698, 787
HpyCH4IV ACGT 1 cut(s) 620
HpyCH4V TGCA 8 cut(s) 574, 582, 773, 881, 907, 1006, 1051, 1084
HpyF10VI GCNNNNNNNGC 2 cut(s) 842, 857
HpyF3I CTNAG 4 cut(s) 207, 294, 444, 1027
HpySE526I ACGT 1 cut(s) 620
Hsp92II CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Kzo9I GATC 2 cut(s) 229, 363
LmnI GCTCC 5 cut(s) 103, 118, 165, 673, 856
Lsp1109I GCAGC 6 cut(s) 322, 688, 785, 958, 1054, 1096
LweI GCATC 2 cut(s) 510, 832
MaeI CTAG 1 cut(s) 626
MaeII ACGT 1 cut(s) 620
MaeIII GTNAC 3 cut(s) 92, 473, 719
MalI GATC 2 cut(s) 231, 365
MboI GATC 2 cut(s) 229, 363
MboII GAAGA 6 cut(s) 207, 395, 641, 659, 968, 982
MfeI CAATTG 1 cut(s) 1007
MhlI GDGCHC 4 cut(s) 123, 162, 1053, 1059
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 6 cut(s) 395, 411, 632, 752, 809, 1007
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 2 cut(s) 765, 878
Mox20I TGGCCA 1 cut(s) 588
MroNI GCCGGC 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 257
MscI TGGCCA 1 cut(s) 588
MseI TTAA 3 cut(s) 114, 927, 951
MslI CAYNNNNRTG 1 cut(s) 1038
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 3 cut(s) 458, 860, 1067
MspI CCGG 3 cut(s) 203, 220, 605
MspR9I CCNGG 3 cut(s) 220, 221, 989
MunI CAATTG 1 cut(s) 1007
Mva1269I GAATGC 1 cut(s) 775
MvaI CCWGG 1 cut(s) 989
MwoI GCNNNNNNNGC 2 cut(s) 842, 857
NaeI GCCGGC 1 cut(s) 204
NciI CCSGG 2 cut(s) 220, 221
NdeII GATC 2 cut(s) 229, 363
NgoMIV GCCGGC 1 cut(s) 202
NlaIII CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
NlaIV GGNNCC 2 cut(s) 564, 852
NmuCI GTSAC 2 cut(s) 473, 719
NspI RCATGY 1 cut(s) 925
NspV TTCGAA 1 cut(s) 53
PctI GAATGC 1 cut(s) 775
PdiI GCCGGC 1 cut(s) 204
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 1 cut(s) 536
PflMI CCANNNNNTGG 3 cut(s) 595, 860, 875
PkrI GCNGC 6 cut(s) 337, 678, 775, 948, 1069, 1086
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
Psp124BI GAGCTC 2 cut(s) 123, 162
Psp6I CCWGG 1 cut(s) 987
PspGI CCWGG 1 cut(s) 987
PspN4I GGNNCC 2 cut(s) 564, 852
PspPI GGNCC 1 cut(s) 407
PstI CTGCAG 1 cut(s) 1086
PvuII CAGCTG 2 cut(s) 458, 860
RseI CAYNNNNRTG 1 cut(s) 1038
SacI GAGCTC 2 cut(s) 123, 162
SaqAI TTAA 3 cut(s) 114, 927, 951
SatI GCNGC 6 cut(s) 336, 677, 774, 947, 1068, 1085
Sau3AI GATC 2 cut(s) 229, 363
Sau96I GGNCC 1 cut(s) 407
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 3 cut(s) 220, 221, 989
SduI GDGCHC 4 cut(s) 123, 162, 1053, 1059
SfaNI GCATC 2 cut(s) 510, 832
SfcI CTRYAG 1 cut(s) 1082
SfuI TTCGAA 1 cut(s) 53
SmaI CCCGGG 1 cut(s) 221
SmiMI CAYNNNNRTG 1 cut(s) 1038
SmlI CTYRAG 2 cut(s) 344, 612
SmoI CTYRAG 2 cut(s) 344, 612
Sse9I AATT 6 cut(s) 395, 411, 632, 752, 809, 1007
SsiI CCGC 4 cut(s) 799, 845, 1043, 1065
SspI AATATT 2 cut(s) 556, 824
SspMI CTAG 1 cut(s) 626
SstI GAGCTC 2 cut(s) 123, 162
StyD4I CCNGG 3 cut(s) 218, 219, 987
StyI CCWWGG 3 cut(s) 193, 514, 816
TaaI ACNGT 5 cut(s) 50, 479, 508, 698, 787
TaiI ACGT 1 cut(s) 623
TaqI TCGA 3 cut(s) 53, 123, 402
TasI AATT 6 cut(s) 395, 411, 632, 752, 809, 1007
TfiI GAWTC 1 cut(s) 536
Tru1I TTAA 3 cut(s) 114, 927, 951
Tru9I TTAA 3 cut(s) 114, 927, 951
TscAI CASTG 6 cut(s) 589, 703, 771, 808, 843, 981
TseFI GTSAC 2 cut(s) 473, 719
TseI GCWGC 6 cut(s) 335, 676, 773, 946, 1067, 1084
Tsp45I GTSAC 2 cut(s) 473, 719
TspDTI ATGAA 2 cut(s) 388, 519
TspGWI ACGGA 3 cut(s) 359, 461, 954
TspMI CCCGGG 1 cut(s) 219
TspRI CASTG 6 cut(s) 589, 703, 771, 808, 843, 981
Van91I CCANNNNNTGG 3 cut(s) 595, 860, 875
VneI GTGCAC 1 cut(s) 1049
XapI RAATTY 2 cut(s) 395, 632
XceI RCATGY 1 cut(s) 925
XmaI CCCGGG 1 cut(s) 219
XmnI GAANNNNTTC 1 cut(s) 257
XspI CTAG 1 cut(s) 626
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.