MD10G1116400.v1.1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009469
Physical Location & Seq
Forward (+)
19350922 .. 19354819
3898 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1116400.v1.1.491

Sequence Viewer

Length: 1047 bp
ATGAAGTTGGTTGGGAACGAGGAGGTGTTTAGTAACTTTGTGGAGCATAAGTTTGAGGAGCTCGACAGGGACAGAGACGGCCACCTCTCTCTGAACGAGCTCCACCCTGCTGTCGCCAATATCGGCCTTGCTCTTGGCTTGCCGGCTCAGGGTTCGTCCCCCGACTCCGATCACATATATTCTGAGGTACTGAATGAATTCACTCATGGGAAGCAACAAAAAGTGAGCAAGACTGAGTTCAAAGAGGTTCTTTCGGATATTCTGCTAGGCATGGCTGCTGGGTTGAAGCGAGACCCTATTGTAATCCTCCGTATTGATGGCGAAGACCTCCTAGAATTCATCGATGGGCCAACTTTTGAATCAGAGATGGTGGCTACTTATTCTGAGATAAAGTCAGCTGATGGAACCCTCCGTGACTGTATAATCAAAGCTTTGGAAAAACTCACGGTTGAACAAGGGATGCCCCCTTCATCAGATTCTTGGGTAGTTGTGAGCAACATTGTGGAACCTGCTCTGCAATCATGCACTGGCCATGATTTGGACAAGCCGGTCTCTCAAGAAACATTCTTAACAGAATTCAAGAAAGTAGCAGAGTCTGTGGCTCATCGTCTTCAGGAGCAGCCTGTGATTGTAGCTCACAGTGAAAATGACTTTGATGGAAGCGACATCAAGAGACTGTTGTCCAACAAGTTTGAATTAGACAAGACACTGAATGCAGCTACTGAAACAATTCCGAGAGACAGCAGTGGGAAATTATCGAAGGAATATTTGCGTGTGGCGCTGGAAGCAGTAGGTCCAACAGCTGGTCTGCCACCACTTGGTGCAGTTGAGCAGATGGATAAGGTTGTGCAGGATGTGTTCAACATGGTTAACGCAGATGATGGAAAGCTGCTTAAAGAAGACGAGTTTAAGAAGTTACTGACTGAAATCCTGGGGAGTATCATGTTGCAATTGGAGGGAAATCTGATTTCAGTTCACTCAAATTCGGTTGTCCACGAGCCCCTCACCTCTTCTTCGACTCTACTGCAGCCAGCTCCCGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

349

Amino Acids

38.18

Weight (kDa)

4.68

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 16 - 84 4.8e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
Acc36I ACCTGC 1 cut(s) 517
AccB7I CCANNNNNTGG 3 cut(s) 538, 803, 818
AcoI YGGCCR 2 cut(s) 79, 529
AcsI RAATTY 4 cut(s) 197, 335, 575, 982
AcuI CTGAAG 1 cut(s) 596
AdeI CACNNNGTG 1 cut(s) 821
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 4 cut(s) 149, 538, 803, 818
AgsI TTSAA 7 cut(s) 241, 286, 359, 452, 580, 695, 862
AjnI CCWGG 1 cut(s) 930
AluBI AGCT 9 cut(s) 61, 100, 398, 431, 635, 719, 803, 889, 1034
AluI AGCT 9 cut(s) 61, 100, 398, 431, 635, 719, 803, 889, 1034
Alw21I GWGCWC 2 cut(s) 63, 102
Alw26I GTCTC 5 cut(s) 69, 285, 556, 667, 732
AlwNI CAGNNNCTG 2 cut(s) 596, 722
Ama87I CYCGRG 1 cut(s) 1037
AoxI GGCC 4 cut(s) 79, 124, 347, 529
ApeKI GCWGC 5 cut(s) 275, 619, 716, 889, 1027
ApoI RAATTY 4 cut(s) 197, 335, 575, 982
Asp700I GAANNNNTTC 2 cut(s) 197, 729
AspLEI GCGC 1 cut(s) 781
AspS9I GGNCC 2 cut(s) 347, 794
AsuHPI GGTGA 1 cut(s) 997
AvaI CYCGRG 1 cut(s) 1037
AvaII GGWCC 1 cut(s) 794
BalI TGGCCA 1 cut(s) 531
BanII GRGCYC 3 cut(s) 63, 102, 1002
BauI CACGAG 1 cut(s) 995
BbsI GAAGAC 3 cut(s) 330, 602, 906
Bbv12I GWGCWC 2 cut(s) 63, 102
BbvI GCAGC 5 cut(s) 262, 631, 728, 876, 1039
BccI CCATC 7 cut(s) 311, 338, 361, 395, 650, 829, 875
BceAI ACGGC 1 cut(s) 94
BcgI CGANNNNNNTGC 2 cut(s) 1006, 1040
BciT130I CCWGG 1 cut(s) 932
BcoDI GTCTC 5 cut(s) 69, 285, 556, 667, 732
BfaI CTAG 2 cut(s) 266, 332
BfmI CTRYAG 1 cut(s) 1025
BfoI RGCGCY 1 cut(s) 782
BfuAI ACCTGC 1 cut(s) 517
BisI GCNGC 5 cut(s) 276, 620, 717, 890, 1028
BlsI GCNGC 5 cut(s) 277, 621, 718, 891, 1029
Bme1390I CCNGG 1 cut(s) 932
Bme18I GGWCC 1 cut(s) 794
BmeT110I CYCGRG 1 cut(s) 1037
BmgT120I GGNCC 2 cut(s) 347, 794
BmiI GGNNCC 2 cut(s) 406, 507
BmrFI CCNGG 1 cut(s) 932
BmsI GCATC 1 cut(s) 450
BoxI GACNNNNGTC 1 cut(s) 679
BpiI GAAGAC 3 cut(s) 330, 602, 906
Bpu10I CCTNAGC 1 cut(s) 147
BpuEI CTTGAG 1 cut(s) 540
Bsa29I ATCGAT 1 cut(s) 342
BsaI GGTCTC 2 cut(s) 285, 556
BsaJI CCNNGG 1 cut(s) 931
BsaXI ACNNNNNCTCC 2 cut(s) 35, 65
Bsc4I CCNNNNNNNGG 4 cut(s) 149, 538, 803, 818
Bse118I RCCGGY 2 cut(s) 142, 547
Bse1I ACTGG 1 cut(s) 532
BseBI CCWGG 1 cut(s) 932
BseCI ATCGAT 1 cut(s) 342
BseDI CCNNGG 1 cut(s) 931
BseGI GGATG 2 cut(s) 465, 859
BseLI CCNNNNNNNGG 4 cut(s) 149, 538, 803, 818
BseMII CTCAG 4 cut(s) 161, 174, 225, 375
BseNI ACTGG 1 cut(s) 532
BseRI GAGGAG 2 cut(s) 35, 71
BseXI GCAGC 5 cut(s) 262, 631, 728, 876, 1039
BseYI CCCAGC 1 cut(s) 278
BsgI GTGCAG 2 cut(s) 843, 869
BshFI GGCC 4 cut(s) 81, 126, 349, 531
BshVI ATCGAT 1 cut(s) 342
BsiHKAI GWGCWC 2 cut(s) 63, 102
BsiHKCI CYCGRG 1 cut(s) 1037
BsiSI CCGG 2 cut(s) 143, 548
BslFI GGGAC 2 cut(s) 83, 142
BslI CCNNNNNNNGG 4 cut(s) 149, 538, 803, 818
BsmAI GTCTC 5 cut(s) 69, 285, 556, 667, 732
BsmBI CGTCTC 1 cut(s) 69
BsmFI GGGAC 2 cut(s) 83, 142
BsmI GAATGC 1 cut(s) 718
BsnI GGCC 4 cut(s) 81, 126, 349, 531
Bso31I GGTCTC 2 cut(s) 285, 556
BsoBI CYCGRG 1 cut(s) 1037
Bsp1286I GDGCHC 3 cut(s) 63, 102, 1002
Bsp143I GATC 1 cut(s) 169
BspANI GGCC 4 cut(s) 81, 126, 349, 531
BspCNI CTCAG 4 cut(s) 160, 175, 226, 376
BspDI ATCGAT 1 cut(s) 342
BspLI GGNNCC 2 cut(s) 406, 507
BspMAI CTGCAG 1 cut(s) 1029
BspMI ACCTGC 1 cut(s) 517
BspTNI GGTCTC 2 cut(s) 285, 556
BsrFI RCCGGY 2 cut(s) 142, 547
BsrI ACTGG 1 cut(s) 532
BssAI RCCGGY 2 cut(s) 142, 547
BssECI CCNNGG 1 cut(s) 931
BssMI GATC 1 cut(s) 169
BssSI CACGAG 1 cut(s) 995
Bst2BI CACGAG 1 cut(s) 995
Bst2UI CCWGG 1 cut(s) 932
Bst4CI ACNGT 4 cut(s) 419, 448, 641, 678
Bst6I CTCTTC 1 cut(s) 1015
BstC8I GCNNGC 3 cut(s) 140, 144, 1032
BstDEI CTNAG 4 cut(s) 147, 183, 234, 384
BstF5I GGATG 2 cut(s) 465, 859
BstH2I RGCGCY 1 cut(s) 782
BstHHI GCGC 1 cut(s) 781
BstKTI GATC 1 cut(s) 172
BstMAI GTCTC 5 cut(s) 69, 285, 556, 667, 732
BstMBI GATC 1 cut(s) 169
BstMWI GCNNNNNNNGC 2 cut(s) 778, 785
BstNI CCWGG 1 cut(s) 932
BstPAI GACNNNNGTC 1 cut(s) 679
BstSCI CCNGG 1 cut(s) 930
BstSFI CTRYAG 1 cut(s) 1025
BstV1I GCAGC 5 cut(s) 262, 631, 728, 876, 1039
BstV2I GAAGAC 3 cut(s) 330, 602, 906
Bsu15I ATCGAT 1 cut(s) 342
BsuRI GGCC 4 cut(s) 81, 126, 349, 531
BsuTUI ATCGAT 1 cut(s) 342
BtsCI GGATG 2 cut(s) 465, 859
BtsI GCAGTG 1 cut(s) 751
BtsIMutI CAGTG 4 cut(s) 525, 646, 707, 751
BveI ACCTGC 1 cut(s) 517
Cac8I GCNNGC 3 cut(s) 140, 144, 1032
CaiI CAGNNNCTG 2 cut(s) 596, 722
CfoI GCGC 1 cut(s) 781
Cfr10I RCCGGY 2 cut(s) 142, 547
Cfr13I GGNCC 2 cut(s) 347, 794
ClaI ATCGAT 1 cut(s) 342
Csp6I GTAC 1 cut(s) 188
CviAII CATG 6 cut(s) 206, 271, 522, 533, 865, 943
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 4 cut(s) 147, 183, 234, 384
DpnI GATC 1 cut(s) 171
DpnII GATC 1 cut(s) 169
DraIII CACNNNGTG 1 cut(s) 821
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
EaeI YGGCCR 2 cut(s) 79, 529
Eam1104I CTCTTC 1 cut(s) 1015
EarI CTCTTC 1 cut(s) 1015
Ecl136II GAGCTC 2 cut(s) 61, 100
Eco24I GRGCYC 3 cut(s) 63, 102, 1002
Eco31I GGTCTC 2 cut(s) 285, 556
Eco47I GGWCC 1 cut(s) 794
Eco53kI GAGCTC 2 cut(s) 61, 100
Eco57I CTGAAG 1 cut(s) 596
Eco88I CYCGRG 1 cut(s) 1037
EcoICRI GAGCTC 2 cut(s) 61, 100
EcoRI GAATTC 3 cut(s) 197, 335, 575
EcoRII CCWGG 1 cut(s) 930
EcoT38I GRGCYC 3 cut(s) 63, 102, 1002
Esp3I CGTCTC 1 cut(s) 69
FaeI CATG 6 cut(s) 209, 274, 525, 536, 868, 946
FalI AAGNNNNNCTT 2 cut(s) 234, 266
FaqI GGGAC 2 cut(s) 83, 142
FatI CATG 6 cut(s) 205, 270, 521, 532, 864, 942
Fnu4HI GCNGC 5 cut(s) 276, 620, 717, 890, 1028
FokI GGATG 2 cut(s) 472, 866
FriOI GRGCYC 3 cut(s) 63, 102, 1002
Fsp4HI GCNGC 5 cut(s) 276, 620, 717, 890, 1028
FspBI CTAG 2 cut(s) 266, 332
GlaI GCGC 1 cut(s) 780
GluI GCNGC 5 cut(s) 276, 620, 717, 890, 1028
GsaI CCCAGC 1 cut(s) 282
HaeII RGCGCY 1 cut(s) 782
HaeIII GGCC 4 cut(s) 81, 126, 349, 531
HapII CCGG 2 cut(s) 143, 548
HhaI GCGC 1 cut(s) 781
Hin1II CATG 6 cut(s) 209, 274, 525, 536, 868, 946
Hin6I GCGC 1 cut(s) 779
HinP1I GCGC 1 cut(s) 779
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HindIII AAGCTT 1 cut(s) 429
HinfI GANTC 5 cut(s) 164, 359, 476, 593, 1018
HpaI GTTAAC 1 cut(s) 871
HpaII CCGG 2 cut(s) 143, 548
HphI GGTGA 1 cut(s) 997
Hpy166II GTNNAC 3 cut(s) 871, 976, 994
Hpy188I TCNGA 9 cut(s) 93, 169, 184, 256, 364, 385, 475, 735, 966
Hpy188III TCNNGA 5 cut(s) 557, 580, 614, 670, 1037
Hpy8I GTNNAC 3 cut(s) 871, 976, 994
HpyAV CCTTC 2 cut(s) 477, 754
HpyCH4III ACNGT 4 cut(s) 419, 448, 641, 678
HpyCH4V TGCA 7 cut(s) 517, 525, 716, 824, 850, 949, 1027
HpyF10VI GCNNNNNNNGC 2 cut(s) 778, 785
HpyF3I CTNAG 4 cut(s) 147, 183, 234, 384
Hsp92II CATG 6 cut(s) 209, 274, 525, 536, 868, 946
HspAI GCGC 1 cut(s) 779
KroI GCCGGC 1 cut(s) 142
KroNI GCCGGC 1 cut(s) 144
KspAI GTTAAC 1 cut(s) 871
Kzo9I GATC 1 cut(s) 169
LmnI GCTCC 5 cut(s) 43, 58, 105, 616, 1039
Lsp1109I GCAGC 5 cut(s) 262, 631, 728, 876, 1039
LweI GCATC 1 cut(s) 450
MaeI CTAG 2 cut(s) 266, 332
MaeIII GTNAC 3 cut(s) 32, 413, 915
MalI GATC 1 cut(s) 171
MboI GATC 1 cut(s) 169
MboII GAAGA 5 cut(s) 335, 602, 911, 1002, 1005
MfeI CAATTG 1 cut(s) 950
MhlI GDGCHC 3 cut(s) 63, 102, 1002
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 8 cut(s) 197, 335, 575, 695, 729, 752, 950, 982
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 3 cut(s) 158, 602, 1012
MmeI TCCRAC 2 cut(s) 708, 821
Mox20I TGGCCA 1 cut(s) 531
MroNI GCCGGC 1 cut(s) 142
MroXI GAANNNNTTC 2 cut(s) 197, 729
MscI TGGCCA 1 cut(s) 531
MseI TTAA 4 cut(s) 569, 870, 894, 909
Msp20I TGGCCA 1 cut(s) 531
MspA1I CMGCKG 2 cut(s) 398, 803
MspI CCGG 2 cut(s) 143, 548
MspR9I CCNGG 1 cut(s) 932
MunI CAATTG 1 cut(s) 950
Mva1269I GAATGC 1 cut(s) 718
MvaI CCWGG 1 cut(s) 932
MwoI GCNNNNNNNGC 2 cut(s) 778, 785
NaeI GCCGGC 1 cut(s) 144
NdeII GATC 1 cut(s) 169
NgoMIV GCCGGC 1 cut(s) 142
NlaIII CATG 6 cut(s) 209, 274, 525, 536, 868, 946
NlaIV GGNNCC 2 cut(s) 406, 507
NmuCI GTSAC 1 cut(s) 413
PctI GAATGC 1 cut(s) 718
PdiI GCCGGC 1 cut(s) 144
PdmI GAANNNNTTC 2 cut(s) 197, 729
PfeI GAWTC 2 cut(s) 359, 476
PflMI CCANNNNNTGG 3 cut(s) 538, 803, 818
PkrI GCNGC 5 cut(s) 277, 621, 718, 891, 1029
PleI GAGTC 3 cut(s) 158, 601, 1012
PpsI GAGTC 3 cut(s) 158, 601, 1012
PshAI GACNNNNGTC 1 cut(s) 679
Psp124BI GAGCTC 2 cut(s) 63, 102
Psp6I CCWGG 1 cut(s) 930
PspFI CCCAGC 1 cut(s) 278
PspGI CCWGG 1 cut(s) 930
PspN4I GGNNCC 2 cut(s) 406, 507
PspPI GGNCC 2 cut(s) 347, 794
PstI CTGCAG 1 cut(s) 1029
PstNI CAGNNNCTG 2 cut(s) 596, 722
PvuII CAGCTG 2 cut(s) 398, 803
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
SacI GAGCTC 2 cut(s) 63, 102
SaqAI TTAA 4 cut(s) 569, 870, 894, 909
SatI GCNGC 5 cut(s) 276, 620, 717, 890, 1028
Sau3AI GATC 1 cut(s) 169
Sau96I GGNCC 2 cut(s) 347, 794
SchI GAGTC 3 cut(s) 158, 602, 1012
ScrFI CCNGG 1 cut(s) 932
SduI GDGCHC 3 cut(s) 63, 102, 1002
SfaNI GCATC 1 cut(s) 450
SfcI CTRYAG 1 cut(s) 1025
SinI GGWCC 1 cut(s) 794
SmlI CTYRAG 1 cut(s) 555
SmoI CTYRAG 1 cut(s) 555
Sse9I AATT 8 cut(s) 197, 335, 575, 695, 729, 752, 950, 982
SspI AATATT 1 cut(s) 767
SspMI CTAG 2 cut(s) 266, 332
SstI GAGCTC 2 cut(s) 63, 102
StyD4I CCNGG 1 cut(s) 930
TaaI ACNGT 4 cut(s) 419, 448, 641, 678
TaqI TCGA 4 cut(s) 63, 342, 758, 1016
TasI AATT 8 cut(s) 197, 335, 575, 695, 729, 752, 950, 982
TfiI GAWTC 2 cut(s) 359, 476
Tru1I TTAA 4 cut(s) 569, 870, 894, 909
Tru9I TTAA 4 cut(s) 569, 870, 894, 909
TscAI CASTG 4 cut(s) 532, 646, 714, 751
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 5 cut(s) 275, 619, 716, 889, 1027
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 4 cut(s) 17, 210, 328, 459
TspGWI ACGGA 2 cut(s) 299, 401
TspRI CASTG 4 cut(s) 532, 646, 714, 751
Van91I CCANNNNNTGG 3 cut(s) 538, 803, 818
VpaK11BI GGWCC 1 cut(s) 794
XapI RAATTY 4 cut(s) 197, 335, 575, 982
XmnI GAANNNNTTC 2 cut(s) 197, 729
XspI CTAG 2 cut(s) 266, 332
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.