pycom10g10110

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr10
OG0009469
Physical Location & Seq
Forward (+)
13387803 .. 13391679
3877 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom10g10110.1

Sequence Viewer

Length: 1104 bp
ATGGAGAAGAAAAGTGGAAGTGGGAGTGGTGGTGGGCAGGTGGTGGACGGTTCAAATATAATGAAATTGGTTGGGAACGAGGAGGTGTTTAGTAACTTTGTGGAGCATAAGTTTGAGGAGCTCGACAGGGACAGAGACGGCCACCTCTCTCTGAACGAGCTCCACCCTGCTGTCGCCGATATCGGCCTTGCTCTTGGCTTGCCGGCTCAGGGTTCGTCCCCCGACTCCGATCACATATATTCTGAGGTACTGAATGAATTCACTCATGGGAAACAACAAAAAGTGAGCAAGACTGAGTTCAAAGAGGTTCTTTCGGATATTCTACTAGGCATGGCTGCTGGGTTGAAGCGAGACCCTATTGTAATCCTCCGTATTGATGGGGAAGACCTCCTAGAATTCATCGATGGGCCAACTTTTGAATCAGAGATGATGGCTACTTATTCTGAGATAAAGTCAGCTGATGGAACCCTCCGTGACTGTTTAATCAAAGCTTTGGAAAAACTCACGGTTGAACAAGGGATGCCCCCTTCATCAGATTCTTGGGTTGTGAGCAATATCGTGGAACCTGCTCTGCAGTCATGCACTGGCCATGATTTGGACAAGACGGTCTCTCAAGAAACATTCTTAGCCGAATTCAAGAAAGTAGCAGAGTTTGTGGCTCATCGTCTTCAGGAGCAACCTGTGATTGTAGCCCACAGTGAAAATGACTTTGATGGAAGCGACATCAAGAGACTGTTGTCCAACAAGTTTGAATTAGACAAGACACTGTATGCAGCTATTGAAACTGTTCCGAGAGACCGCAATGGGAAATTATCCAAGGAATATTTGCGCTTGGCGCTGGAAGCAGTAGGTCCAACAGCTGGTCTGCCACCACTTGGTGCAATTGAGCAGATGGATAAGGTTGTGCAGGATGCATTCAACATGGTTAATGCAGATGACGGAAAGCTGCTTAAAGAAGACGAGTTCAAGAAGTTACTGACTGAAATCCTGGGGAGTATCATGTTGCAATTGGAGGGAAATCCGATTTCAGTTCACTCTAATTCGGTTGTCCACGAGCCCCTCAACGCTTCTTCTACTCTACTGCAGCCAACTCCCGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

368

Amino Acids

40.18

Weight (kDa)

4.7

Isoelectric Point (pI)

44.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 36 - 104 1.9e-08 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
AasI GACNNNNNNGTC 1 cut(s) 605
Acc36I ACCTGC 2 cut(s) 28, 574
AccB7I CCANNNNNTGG 3 cut(s) 595, 860, 875
AciI CCGC 1 cut(s) 799
AcoI YGGCCR 2 cut(s) 139, 586
AcsI RAATTY 3 cut(s) 257, 395, 632
AcuI CTGAAG 1 cut(s) 653
AdeI CACNNNGTG 1 cut(s) 878
AfaI GTAC 1 cut(s) 249
AfiI CCNNNNNNNGG 4 cut(s) 209, 595, 860, 875
AjnI CCWGG 1 cut(s) 987
AluBI AGCT 7 cut(s) 121, 160, 458, 491, 776, 860, 946
AluI AGCT 7 cut(s) 121, 160, 458, 491, 776, 860, 946
Alw21I GWGCWC 2 cut(s) 123, 162
Alw26I GTCTC 5 cut(s) 129, 345, 613, 724, 789
Ama87I CYCGRG 1 cut(s) 1094
AoxI GGCC 4 cut(s) 139, 184, 407, 586
ApeKI GCWGC 4 cut(s) 335, 773, 946, 1084
ApoI RAATTY 3 cut(s) 257, 395, 632
Asp700I GAANNNNTTC 2 cut(s) 257, 786
AspLEI GCGC 2 cut(s) 831, 838
AspS9I GGNCC 2 cut(s) 407, 851
AvaI CYCGRG 1 cut(s) 1094
AvaII GGWCC 1 cut(s) 851
BalI TGGCCA 1 cut(s) 588
BanII GRGCYC 3 cut(s) 123, 162, 1059
BauI CACGAG 1 cut(s) 1052
BbsI GAAGAC 3 cut(s) 390, 659, 963
Bbv12I GWGCWC 2 cut(s) 123, 162
BbvI GCAGC 4 cut(s) 322, 785, 933, 1096
BccI CCATC 6 cut(s) 371, 398, 424, 455, 707, 886
BceAI ACGGC 1 cut(s) 154
BciT130I CCWGG 1 cut(s) 989
BcoDI GTCTC 5 cut(s) 129, 345, 613, 724, 789
BfaI CTAG 2 cut(s) 326, 392
BfmI CTRYAG 2 cut(s) 572, 1082
BfoI RGCGCY 1 cut(s) 839
BfuAI ACCTGC 2 cut(s) 28, 574
BisI GCNGC 4 cut(s) 336, 774, 947, 1085
BlsI GCNGC 4 cut(s) 337, 775, 948, 1086
Bme1390I CCNGG 1 cut(s) 989
Bme18I GGWCC 1 cut(s) 851
BmeT110I CYCGRG 1 cut(s) 1094
BmgT120I GGNCC 2 cut(s) 407, 851
BmiI GGNNCC 2 cut(s) 466, 564
BmrFI CCNGG 1 cut(s) 989
BmsI GCATC 2 cut(s) 510, 901
BoxI GACNNNNGTC 1 cut(s) 736
BpiI GAAGAC 3 cut(s) 390, 659, 963
Bpu10I CCTNAGC 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 597
Bsa29I ATCGAT 1 cut(s) 402
BsaI GGTCTC 3 cut(s) 345, 613, 789
BsaJI CCNNGG 2 cut(s) 816, 988
BsaXI ACNNNNNCTCC 4 cut(s) 16, 46, 95, 125
Bsc4I CCNNNNNNNGG 4 cut(s) 209, 595, 860, 875
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 589
Bse3DI GCAATG 1 cut(s) 808
BseBI CCWGG 1 cut(s) 989
BseCI ATCGAT 1 cut(s) 402
BseDI CCNNGG 2 cut(s) 816, 988
BseGI GGATG 2 cut(s) 525, 916
BseLI CCNNNNNNNGG 4 cut(s) 209, 595, 860, 875
BseMI GCAATG 1 cut(s) 808
BseMII CTCAG 4 cut(s) 221, 234, 285, 435
BseNI ACTGG 1 cut(s) 589
BseRI GAGGAG 2 cut(s) 95, 131
BseXI GCAGC 4 cut(s) 322, 785, 933, 1096
BseYI CCCAGC 1 cut(s) 338
BsgI GTGCAG 1 cut(s) 926
BshFI GGCC 4 cut(s) 141, 186, 409, 588
BshVI ATCGAT 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 123, 162
BsiHKCI CYCGRG 1 cut(s) 1094
BsiSI CCGG 1 cut(s) 203
BslFI GGGAC 2 cut(s) 143, 202
BslI CCNNNNNNNGG 4 cut(s) 209, 595, 860, 875
BsmAI GTCTC 5 cut(s) 129, 345, 613, 724, 789
BsmBI CGTCTC 1 cut(s) 129
BsmFI GGGAC 2 cut(s) 143, 202
BsmI GAATGC 1 cut(s) 914
BsnI GGCC 4 cut(s) 141, 186, 409, 588
Bso31I GGTCTC 3 cut(s) 345, 613, 789
BsoBI CYCGRG 1 cut(s) 1094
Bsp1286I GDGCHC 3 cut(s) 123, 162, 1059
Bsp143I GATC 1 cut(s) 229
BspACI CCGC 1 cut(s) 799
BspANI GGCC 4 cut(s) 141, 186, 409, 588
BspCNI CTCAG 4 cut(s) 220, 235, 286, 436
BspDI ATCGAT 1 cut(s) 402
BspLI GGNNCC 2 cut(s) 466, 564
BspMAI CTGCAG 2 cut(s) 576, 1086
BspMI ACCTGC 2 cut(s) 28, 574
BspTNI GGTCTC 3 cut(s) 345, 613, 789
BsrDI GCAATG 1 cut(s) 808
BsrFI RCCGGY 1 cut(s) 202
BsrI ACTGG 1 cut(s) 589
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 816, 988
BssMI GATC 1 cut(s) 229
BssSI CACGAG 1 cut(s) 1052
BssT1I CCWWGG 1 cut(s) 816
Bst2BI CACGAG 1 cut(s) 1052
Bst2UI CCWGG 1 cut(s) 989
Bst4CI ACNGT 8 cut(s) 50, 479, 508, 607, 698, 735, 768, 787
BstC8I GCNNGC 2 cut(s) 200, 204
BstDEI CTNAG 5 cut(s) 207, 243, 294, 444, 625
BstF5I GGATG 2 cut(s) 525, 916
BstH2I RGCGCY 1 cut(s) 839
BstHHI GCGC 2 cut(s) 831, 838
BstKTI GATC 1 cut(s) 232
BstMAI GTCTC 5 cut(s) 129, 345, 613, 724, 789
BstMBI GATC 1 cut(s) 229
BstMWI GCNNNNNNNGC 2 cut(s) 835, 842
BstNI CCWGG 1 cut(s) 989
BstPAI GACNNNNGTC 1 cut(s) 736
BstSCI CCNGG 1 cut(s) 987
BstSFI CTRYAG 2 cut(s) 572, 1082
BstV1I GCAGC 4 cut(s) 322, 785, 933, 1096
BstV2I GAAGAC 3 cut(s) 390, 659, 963
Bsu15I ATCGAT 1 cut(s) 402
BsuRI GGCC 4 cut(s) 141, 186, 409, 588
BsuTUI ATCGAT 1 cut(s) 402
BtsCI GGATG 2 cut(s) 525, 916
BtsIMutI CAGTG 3 cut(s) 582, 703, 764
BveI ACCTGC 2 cut(s) 28, 574
Cac8I GCNNGC 2 cut(s) 200, 204
CfoI GCGC 2 cut(s) 831, 838
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 2 cut(s) 407, 851
ClaI ATCGAT 1 cut(s) 402
Csp6I GTAC 1 cut(s) 248
CviAII CATG 6 cut(s) 266, 331, 579, 590, 922, 1000
CviQI GTAC 1 cut(s) 248
DdeI CTNAG 5 cut(s) 207, 243, 294, 444, 625
DpnI GATC 1 cut(s) 231
DpnII GATC 1 cut(s) 229
DraIII CACNNNGTG 1 cut(s) 878
DrdI GACNNNNNNGTC 1 cut(s) 605
DseDI GACNNNNNNGTC 1 cut(s) 605
EaeI YGGCCR 2 cut(s) 139, 586
Ecl136II GAGCTC 2 cut(s) 121, 160
Eco130I CCWWGG 1 cut(s) 816
Eco24I GRGCYC 3 cut(s) 123, 162, 1059
Eco31I GGTCTC 3 cut(s) 345, 613, 789
Eco32I GATATC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 851
Eco53kI GAGCTC 2 cut(s) 121, 160
Eco57I CTGAAG 1 cut(s) 653
Eco88I CYCGRG 1 cut(s) 1094
EcoICRI GAGCTC 2 cut(s) 121, 160
EcoRI GAATTC 3 cut(s) 257, 395, 632
EcoRII CCWGG 1 cut(s) 987
EcoRV GATATC 1 cut(s) 181
EcoT14I CCWWGG 1 cut(s) 816
EcoT22I ATGCAT 1 cut(s) 916
EcoT38I GRGCYC 3 cut(s) 123, 162, 1059
ErhI CCWWGG 1 cut(s) 816
Esp3I CGTCTC 1 cut(s) 129
FaeI CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FaqI GGGAC 2 cut(s) 143, 202
FatI CATG 6 cut(s) 265, 330, 578, 589, 921, 999
Fnu4HI GCNGC 4 cut(s) 336, 774, 947, 1085
FokI GGATG 2 cut(s) 532, 923
FriOI GRGCYC 3 cut(s) 123, 162, 1059
Fsp4HI GCNGC 4 cut(s) 336, 774, 947, 1085
FspBI CTAG 2 cut(s) 326, 392
GlaI GCGC 2 cut(s) 830, 837
GluI GCNGC 4 cut(s) 336, 774, 947, 1085
GsaI CCCAGC 1 cut(s) 342
HaeII RGCGCY 1 cut(s) 839
HaeIII GGCC 4 cut(s) 141, 186, 409, 588
HapII CCGG 1 cut(s) 203
HhaI GCGC 2 cut(s) 831, 838
Hin1II CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
Hin6I GCGC 2 cut(s) 829, 836
HinP1I GCGC 2 cut(s) 829, 836
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 3 cut(s) 224, 419, 536
HpaII CCGG 1 cut(s) 203
Hpy166II GTNNAC 3 cut(s) 46, 1033, 1051
Hpy188I TCNGA 9 cut(s) 153, 229, 244, 316, 424, 445, 535, 792, 1023
Hpy188III TCNNGA 6 cut(s) 614, 637, 671, 727, 967, 1094
Hpy8I GTNNAC 3 cut(s) 46, 1033, 1051
HpyAV CCTTC 1 cut(s) 537
HpyCH4III ACNGT 8 cut(s) 50, 479, 508, 607, 698, 735, 768, 787
HpyCH4V TGCA 9 cut(s) 574, 582, 773, 881, 907, 914, 932, 1006, 1084
HpyF10VI GCNNNNNNNGC 2 cut(s) 835, 842
HpyF3I CTNAG 5 cut(s) 207, 243, 294, 444, 625
Hsp92II CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
HspAI GCGC 2 cut(s) 829, 836
KroI GCCGGC 1 cut(s) 202
KroNI GCCGGC 1 cut(s) 204
Kzo9I GATC 1 cut(s) 229
LmnI GCTCC 4 cut(s) 103, 118, 165, 673
Lsp1109I GCAGC 4 cut(s) 322, 785, 933, 1096
LweI GCATC 2 cut(s) 510, 901
MaeI CTAG 2 cut(s) 326, 392
MaeIII GTNAC 3 cut(s) 92, 473, 972
MalI GATC 1 cut(s) 231
MboI GATC 1 cut(s) 229
MboII GAAGA 5 cut(s) 19, 395, 659, 968, 1062
MfeI CAATTG 2 cut(s) 882, 1007
MhlI GDGCHC 3 cut(s) 123, 162, 1059
MlsI TGGCCA 1 cut(s) 588
MluCI AATT 9 cut(s) 65, 257, 395, 632, 752, 809, 882, 1007, 1039
MluNI TGGCCA 1 cut(s) 588
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 2 cut(s) 765, 878
Mox20I TGGCCA 1 cut(s) 588
Mph1103I ATGCAT 1 cut(s) 916
MroNI GCCGGC 1 cut(s) 202
MroXI GAANNNNTTC 2 cut(s) 257, 786
MscI TGGCCA 1 cut(s) 588
MseI TTAA 3 cut(s) 482, 927, 951
Msp20I TGGCCA 1 cut(s) 588
MspA1I CMGCKG 2 cut(s) 458, 860
MspI CCGG 1 cut(s) 203
MspR9I CCNGG 1 cut(s) 989
MunI CAATTG 2 cut(s) 882, 1007
Mva1269I GAATGC 1 cut(s) 914
MvaI CCWGG 1 cut(s) 989
MwoI GCNNNNNNNGC 2 cut(s) 835, 842
NaeI GCCGGC 1 cut(s) 204
NdeII GATC 1 cut(s) 229
NgoMIV GCCGGC 1 cut(s) 202
NlaIII CATG 6 cut(s) 269, 334, 582, 593, 925, 1003
NlaIV GGNNCC 2 cut(s) 466, 564
NmuCI GTSAC 1 cut(s) 473
NsiI ATGCAT 1 cut(s) 916
PaqCI CACCTGC 1 cut(s) 28
PctI GAATGC 1 cut(s) 914
PdiI GCCGGC 1 cut(s) 204
PdmI GAANNNNTTC 2 cut(s) 257, 786
PfeI GAWTC 2 cut(s) 419, 536
PflMI CCANNNNNTGG 3 cut(s) 595, 860, 875
PkrI GCNGC 4 cut(s) 337, 775, 948, 1086
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PshAI GACNNNNGTC 1 cut(s) 736
Psp124BI GAGCTC 2 cut(s) 123, 162
Psp6I CCWGG 1 cut(s) 987
PspFI CCCAGC 1 cut(s) 338
PspGI CCWGG 1 cut(s) 987
PspN4I GGNNCC 2 cut(s) 466, 564
PspPI GGNCC 2 cut(s) 407, 851
PstI CTGCAG 2 cut(s) 576, 1086
PvuII CAGCTG 2 cut(s) 458, 860
RsaI GTAC 1 cut(s) 249
RsaNI GTAC 1 cut(s) 248
SacI GAGCTC 2 cut(s) 123, 162
SaqAI TTAA 3 cut(s) 482, 927, 951
SatI GCNGC 4 cut(s) 336, 774, 947, 1085
Sau3AI GATC 1 cut(s) 229
Sau96I GGNCC 2 cut(s) 407, 851
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 989
SduI GDGCHC 3 cut(s) 123, 162, 1059
SfaNI GCATC 2 cut(s) 510, 901
SfcI CTRYAG 2 cut(s) 572, 1082
SinI GGWCC 1 cut(s) 851
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
Sse9I AATT 9 cut(s) 65, 257, 395, 632, 752, 809, 882, 1007, 1039
SsiI CCGC 1 cut(s) 799
SspI AATATT 1 cut(s) 824
SspMI CTAG 2 cut(s) 326, 392
SstI GAGCTC 2 cut(s) 123, 162
StyD4I CCNGG 1 cut(s) 987
StyI CCWWGG 1 cut(s) 816
TaaI ACNGT 8 cut(s) 50, 479, 508, 607, 698, 735, 768, 787
TaqI TCGA 2 cut(s) 123, 402
TasI AATT 9 cut(s) 65, 257, 395, 632, 752, 809, 882, 1007, 1039
TfiI GAWTC 2 cut(s) 419, 536
Tru1I TTAA 3 cut(s) 482, 927, 951
Tru9I TTAA 3 cut(s) 482, 927, 951
TscAI CASTG 3 cut(s) 589, 703, 771
TseFI GTSAC 1 cut(s) 473
TseI GCWGC 4 cut(s) 335, 773, 946, 1084
Tsp45I GTSAC 1 cut(s) 473
TspDTI ATGAA 4 cut(s) 77, 270, 388, 519
TspGWI ACGGA 3 cut(s) 359, 461, 954
TspRI CASTG 3 cut(s) 589, 703, 771
Van91I CCANNNNNTGG 3 cut(s) 595, 860, 875
VpaK11BI GGWCC 1 cut(s) 851
XapI RAATTY 3 cut(s) 257, 395, 632
XmnI GAANNNNTTC 2 cut(s) 257, 786
XspI CTAG 2 cut(s) 326, 392
Zsp2I ATGCAT 1 cut(s) 916
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.