Prupe.8G158800_v2.0.a1

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp08
OG0009469
Physical Location & Seq
Forward (+)
16830732 .. 16834878
4147 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.8G158800.1

Sequence Viewer

Length: 1110 bp
ATGGAGGAGAGCCCAAGGAAGAAAAGTGGAGCGGGGCAGGTGCTGGACGGTTCGAATATAATGGAGTTGGTTGGGAACAAGGAGGTGTTCAGTAGTTTTGTGGAGCATAAGTTTAAGGAGCTGGATAGAGACAGAGATGGTGAGCTATCTGTGAAGGAGCTTCAGCCTGCTGTTGCTGATATTGGCGCTGCTCTTGGCTTGCCTGCTCAGGGCTCTTCCCCTGACTCTGATCACATCTATTCTGAGGTGTTGAATGAGTTCACTCATGGAAAACAACAAAAAGTGAGCAAGACTGAGTTCAAAGAGGTTCTTTCAGATATTCTGTTAGGCATGGCTGCTGGTTTGAAGCGAGACCCTATTGTGATCCTCCGTATTGATGGAGAAGATCTCGAAGAATTCATCAATGGGCCAACTTTTGAACCAGAGATGGTAGCCTCGTATTCTGAGATGAAGTCAGCTGATGGAAGTGGAAGCCTCCGTGACTATATAGCCAAGGCTTTGGCAAAACTCACCGTTGAGCAAGGGATGCCCCCTTCATCAGATCCTTGGGTTATTAGCAATATCGTGGAACCTGCTCTGCAATCATGCACTGGCCATGATTTTGACAAGCCTGTATCTCAAGAAACTTTCATAGAGGAGTTTAAGAAAGTGGCAGAAACCGTGGCTCAACACCTTAAGGAGCAGCATGTGATTGTAGCCCACAGTGAAAATGACTTTGATGGAAGTGGCATTAAGAGACTGTTGTCCAACAAGTTTGAATTAGACAAGACACTGAATACAGCTATTGAAAATGTGTCAAAAGATCGCAGTGGGAAACTGTCCAAGGAATATTTGCGTGTGGCGCTGGATGCAGTGGGTCCAACCGCTGGTCTGCCACCACTTGGTGCAGTTGAGCAGATGGACAAGGTTGTGCAGGACGCGTTCAACTTGGTGAATGCAGATGATGGAAAGCTGCTCAAAGAAGACGAGTTCAAAAAGATATTGACTGAAATCCTGGGGAGCATCATGTTGCAATTGGAGGGAAATCCTATCTCAGTTTACTCAAATTCGGTTGTGCATGAGCCCCTTGCCTCCTCTTCTACACTATTGCAGCCATCTTCCGAGTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

370

Amino Acids

40.25

Weight (kDa)

4.75

Isoelectric Point (pI)

47.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 28
Acc36I ACCTGC 2 cut(s) 28, 580
AccB7I CCANNNNNTGG 2 cut(s) 866, 881
AccBSI CCGCTC 1 cut(s) 32
AccII CGCG 1 cut(s) 920
AciI CCGC 2 cut(s) 32, 864
AclWI GGATC 2 cut(s) 358, 536
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 2 cut(s) 395, 1045
AcuI CTGAAG 1 cut(s) 146
AdeI CACNNNGTG 1 cut(s) 884
AfiI CCNNNNNNNGG 3 cut(s) 209, 866, 881
AflII CTTAAG 1 cut(s) 674
AflIII ACRYGT 1 cut(s) 918
AgsI TTSAA 8 cut(s) 253, 301, 346, 419, 758, 788, 925, 973
AjnI CCWGG 1 cut(s) 993
AluBI AGCT 6 cut(s) 121, 145, 160, 458, 782, 952
AluI AGCT 6 cut(s) 121, 145, 160, 458, 782, 952
Alw26I GTCTC 3 cut(s) 123, 345, 730
AlwI GGATC 2 cut(s) 358, 536
AlwNI CAGNNNCTG 1 cut(s) 43
AoxI GGCC 2 cut(s) 407, 592
ApeKI GCWGC 5 cut(s) 188, 335, 682, 952, 1090
ApoI RAATTY 2 cut(s) 395, 1045
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 2 cut(s) 188, 844
AspS9I GGNCC 2 cut(s) 407, 857
AsuHPI GGTGA 3 cut(s) 152, 502, 943
AsuII TTCGAA 1 cut(s) 53
AvaII GGWCC 1 cut(s) 857
BalI TGGCCA 1 cut(s) 594
BanII GRGCYC 3 cut(s) 14, 215, 1065
BbsI GAAGAC 1 cut(s) 969
BbvI GCAGC 5 cut(s) 175, 322, 694, 939, 1102
BccI CCATC 8 cut(s) 131, 371, 421, 455, 713, 892, 938, 1102
BciT130I CCWGG 1 cut(s) 995
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 3 cut(s) 123, 345, 730
BfoI RGCGCY 2 cut(s) 189, 845
BfrI CTTAAG 1 cut(s) 674
BfuAI ACCTGC 2 cut(s) 28, 580
BglII AGATCT 1 cut(s) 385
BisI GCNGC 5 cut(s) 189, 336, 683, 953, 1091
BlsI GCNGC 5 cut(s) 190, 337, 684, 954, 1092
Bme1390I CCNGG 1 cut(s) 995
Bme18I GGWCC 1 cut(s) 857
BmgT120I GGNCC 2 cut(s) 407, 857
BmiI GGNNCC 2 cut(s) 570, 858
BmrFI CCNGG 1 cut(s) 995
BmsI GCATC 3 cut(s) 516, 838, 1011
BoxI GACNNNNGTC 1 cut(s) 742
BpiI GAAGAC 1 cut(s) 969
BplI GAGNNNNNCTC 2 cut(s) 372, 404
Bpu10I CCTNAGC 1 cut(s) 207
Bpu14I TTCGAA 1 cut(s) 53
BpuEI CTTGAG 1 cut(s) 603
BsaI GGTCTC 1 cut(s) 345
BsaJI CCNNGG 6 cut(s) 14, 492, 545, 660, 822, 994
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 3 cut(s) 209, 866, 881
Bse1I ACTGG 1 cut(s) 595
BseBI CCWGG 1 cut(s) 995
BseDI CCNNGG 6 cut(s) 14, 492, 545, 660, 822, 994
BseGI GGATG 2 cut(s) 531, 853
BseLI CCNNNNNNNGG 3 cut(s) 209, 866, 881
BseMII CTCAG 5 cut(s) 221, 234, 285, 435, 1047
BseNI ACTGG 1 cut(s) 595
BseRI GAGGAG 3 cut(s) 20, 650, 1063
BseXI GCAGC 5 cut(s) 175, 322, 694, 939, 1102
BsgI GTGCAG 2 cut(s) 906, 932
Bsh1236I CGCG 1 cut(s) 920
BshFI GGCC 2 cut(s) 409, 594
BslI CCNNNNNNNGG 3 cut(s) 209, 866, 881
BsmAI GTCTC 3 cut(s) 123, 345, 730
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 2 cut(s) 409, 594
Bso31I GGTCTC 1 cut(s) 345
Bsp119I TTCGAA 1 cut(s) 53
Bsp1286I GDGCHC 3 cut(s) 14, 215, 1065
Bsp143I GATC 5 cut(s) 229, 363, 385, 541, 802
BspACI CCGC 2 cut(s) 32, 864
BspANI GGCC 2 cut(s) 409, 594
BspCNI CTCAG 5 cut(s) 220, 235, 286, 436, 1046
BspFNI CGCG 1 cut(s) 920
BspLI GGNNCC 2 cut(s) 570, 858
BspMI ACCTGC 2 cut(s) 28, 580
BspPI GGATC 2 cut(s) 358, 536
BspQI GCTCTTC 1 cut(s) 220
BspT104I TTCGAA 1 cut(s) 53
BspTI CTTAAG 1 cut(s) 674
BspTNI GGTCTC 1 cut(s) 345
BsrBI CCGCTC 1 cut(s) 32
BsrI ACTGG 1 cut(s) 595
BssECI CCNNGG 6 cut(s) 14, 492, 545, 660, 822, 994
BssMI GATC 5 cut(s) 229, 363, 385, 541, 802
BssT1I CCWWGG 4 cut(s) 14, 492, 545, 822
Bst2UI CCWGG 1 cut(s) 995
Bst4CI ACNGT 6 cut(s) 50, 514, 661, 704, 741, 819
Bst6I CTCTTC 2 cut(s) 220, 1081
BstAFI CTTAAG 1 cut(s) 674
BstAPI GCANNNNNTGC 1 cut(s) 526
BstBI TTCGAA 1 cut(s) 53
BstC8I GCNNGC 3 cut(s) 168, 200, 204
BstDEI CTNAG 5 cut(s) 207, 243, 294, 444, 1033
BstDSI CCRYGG 1 cut(s) 660
BstF5I GGATG 2 cut(s) 531, 853
BstFNI CGCG 1 cut(s) 920
BstH2I RGCGCY 2 cut(s) 189, 845
BstHHI GCGC 2 cut(s) 188, 844
BstKTI GATC 5 cut(s) 232, 366, 388, 544, 805
BstMAI GTCTC 3 cut(s) 123, 345, 730
BstMBI GATC 5 cut(s) 229, 363, 385, 541, 802
BstMWI GCNNNNNNNGC 3 cut(s) 526, 841, 848
BstNI CCWGG 1 cut(s) 995
BstNSI RCATGY 1 cut(s) 689
BstPAI GACNNNNGTC 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 993
BstUI CGCG 1 cut(s) 920
BstV1I GCAGC 5 cut(s) 175, 322, 694, 939, 1102
BstV2I GAAGAC 1 cut(s) 969
BstX2I RGATCY 2 cut(s) 385, 541
BstXI CCANNNNNNTGG 1 cut(s) 499
BstYI RGATCY 2 cut(s) 385, 541
BsuRI GGCC 2 cut(s) 409, 594
BtgI CCRYGG 1 cut(s) 660
BtsCI GGATG 2 cut(s) 531, 853
BtsI GCAGTG 2 cut(s) 814, 858
BtsIMutI CAGTG 5 cut(s) 588, 709, 770, 814, 858
BveI ACCTGC 2 cut(s) 28, 580
Cac8I GCNNGC 3 cut(s) 168, 200, 204
CaiI CAGNNNCTG 1 cut(s) 43
CfoI GCGC 2 cut(s) 188, 844
Cfr13I GGNCC 2 cut(s) 407, 857
CseI GACGC 1 cut(s) 926
CviAII CATG 7 cut(s) 266, 331, 585, 596, 686, 1006, 1058
DdeI CTNAG 5 cut(s) 207, 243, 294, 444, 1033
DpnI GATC 5 cut(s) 231, 365, 387, 543, 804
DpnII GATC 5 cut(s) 229, 363, 385, 541, 802
DraIII CACNNNGTG 1 cut(s) 884
EaeI YGGCCR 1 cut(s) 592
Eam1104I CTCTTC 2 cut(s) 220, 1081
EarI CTCTTC 2 cut(s) 220, 1081
Eco130I CCWWGG 4 cut(s) 14, 492, 545, 822
Eco24I GRGCYC 3 cut(s) 14, 215, 1065
Eco31I GGTCTC 1 cut(s) 345
Eco47I GGWCC 1 cut(s) 857
Eco57I CTGAAG 1 cut(s) 146
EcoRI GAATTC 1 cut(s) 395
EcoRII CCWGG 1 cut(s) 993
EcoT14I CCWWGG 4 cut(s) 14, 492, 545, 822
EcoT38I GRGCYC 3 cut(s) 14, 215, 1065
ErhI CCWWGG 4 cut(s) 14, 492, 545, 822
FaeI CATG 7 cut(s) 269, 334, 588, 599, 689, 1009, 1061
FalI AAGNNNNNCTT 2 cut(s) 294, 326
FatI CATG 7 cut(s) 265, 330, 584, 595, 685, 1005, 1057
FauI CCCGC 1 cut(s) 25
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 5 cut(s) 189, 336, 683, 953, 1091
FokI GGATG 2 cut(s) 538, 860
FriOI GRGCYC 3 cut(s) 14, 215, 1065
Fsp4HI GCNGC 5 cut(s) 189, 336, 683, 953, 1091
GlaI GCGC 2 cut(s) 187, 843
GluI GCNGC 5 cut(s) 189, 336, 683, 953, 1091
HaeII RGCGCY 2 cut(s) 189, 845
HaeIII GGCC 2 cut(s) 409, 594
HgaI GACGC 1 cut(s) 926
HhaI GCGC 2 cut(s) 188, 844
Hin1II CATG 7 cut(s) 269, 334, 588, 599, 689, 1009, 1061
Hin6I GCGC 2 cut(s) 186, 842
HinP1I GCGC 2 cut(s) 186, 842
HinfI GANTC 1 cut(s) 224
HphI GGTGA 3 cut(s) 152, 502, 943
Hpy166II GTNNAC 2 cut(s) 261, 1039
Hpy188I TCNGA 6 cut(s) 229, 244, 316, 445, 541, 1102
Hpy188III TCNNGA 2 cut(s) 389, 620
Hpy8I GTNNAC 2 cut(s) 261, 1039
HpyAV CCTTC 2 cut(s) 148, 543
HpyCH4III ACNGT 6 cut(s) 50, 514, 661, 704, 741, 819
HpyCH4V TGCA 9 cut(s) 580, 588, 851, 887, 913, 938, 1012, 1057, 1090
HpyF10VI GCNNNNNNNGC 3 cut(s) 526, 841, 848
HpyF3I CTNAG 5 cut(s) 207, 243, 294, 444, 1033
Hsp92II CATG 7 cut(s) 269, 334, 588, 599, 689, 1009, 1061
HspAI GCGC 2 cut(s) 186, 842
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 5 cut(s) 229, 363, 385, 541, 802
LguI GCTCTTC 1 cut(s) 220
LmnI GCTCC 6 cut(s) 29, 103, 118, 157, 679, 999
Lsp1109I GCAGC 5 cut(s) 175, 322, 694, 939, 1102
LweI GCATC 3 cut(s) 516, 838, 1011
MaeIII GTNAC 1 cut(s) 479
MalI GATC 5 cut(s) 231, 365, 387, 543, 804
MbiI CCGCTC 1 cut(s) 32
MboI GATC 5 cut(s) 229, 363, 385, 541, 802
MboII GAAGA 7 cut(s) 31, 207, 395, 404, 974, 1068, 1089
MfeI CAATTG 1 cut(s) 1013
MflI RGATCY 2 cut(s) 385, 541
MhlI GDGCHC 3 cut(s) 14, 215, 1065
MlsI TGGCCA 1 cut(s) 594
MluCI AATT 4 cut(s) 395, 758, 1013, 1045
MluI ACGCGT 1 cut(s) 918
MluNI TGGCCA 1 cut(s) 594
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 2 cut(s) 771, 884
Mox20I TGGCCA 1 cut(s) 594
MroXI GAANNNNTTC 1 cut(s) 257
MscI TGGCCA 1 cut(s) 594
MseI TTAA 4 cut(s) 114, 642, 675, 732
Msp20I TGGCCA 1 cut(s) 594
MspA1I CMGCKG 2 cut(s) 458, 866
MspCI CTTAAG 1 cut(s) 674
MspR9I CCNGG 1 cut(s) 995
MunI CAATTG 1 cut(s) 1013
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 1 cut(s) 995
MvnI CGCG 1 cut(s) 920
MwoI GCNNNNNNNGC 3 cut(s) 526, 841, 848
NdeII GATC 5 cut(s) 229, 363, 385, 541, 802
NlaIII CATG 7 cut(s) 269, 334, 588, 599, 689, 1009, 1061
NlaIV GGNNCC 2 cut(s) 570, 858
NmuCI GTSAC 1 cut(s) 479
NspI RCATGY 1 cut(s) 689
NspV TTCGAA 1 cut(s) 53
PaqCI CACCTGC 1 cut(s) 28
PciSI GCTCTTC 1 cut(s) 220
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 257
PflMI CCANNNNNTGG 2 cut(s) 866, 881
PkrI GCNGC 5 cut(s) 190, 337, 684, 954, 1092
PleI GAGTC 1 cut(s) 218
PpsI GAGTC 1 cut(s) 218
PshAI GACNNNNGTC 1 cut(s) 742
Psp6I CCWGG 1 cut(s) 993
PspGI CCWGG 1 cut(s) 993
PspN4I GGNNCC 2 cut(s) 570, 858
PspPI GGNCC 2 cut(s) 407, 857
PstNI CAGNNNCTG 1 cut(s) 43
PsuI RGATCY 2 cut(s) 385, 541
PvuII CAGCTG 1 cut(s) 458
SapI GCTCTTC 1 cut(s) 220
SaqAI TTAA 4 cut(s) 114, 642, 675, 732
SatI GCNGC 5 cut(s) 189, 336, 683, 953, 1091
Sau3AI GATC 5 cut(s) 229, 363, 385, 541, 802
Sau96I GGNCC 2 cut(s) 407, 857
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 1 cut(s) 995
SduI GDGCHC 3 cut(s) 14, 215, 1065
SfaNI GCATC 3 cut(s) 516, 838, 1011
SfuI TTCGAA 1 cut(s) 53
SinI GGWCC 1 cut(s) 857
SmlI CTYRAG 2 cut(s) 618, 674
SmoI CTYRAG 2 cut(s) 618, 674
Sse9I AATT 4 cut(s) 395, 758, 1013, 1045
SsiI CCGC 2 cut(s) 32, 864
SspI AATATT 1 cut(s) 830
StyD4I CCNGG 1 cut(s) 993
StyI CCWWGG 4 cut(s) 14, 492, 545, 822
TaaI ACNGT 6 cut(s) 50, 514, 661, 704, 741, 819
TaqI TCGA 2 cut(s) 53, 390
TasI AATT 4 cut(s) 395, 758, 1013, 1045
Tru1I TTAA 4 cut(s) 114, 642, 675, 732
Tru9I TTAA 4 cut(s) 114, 642, 675, 732
TscAI CASTG 5 cut(s) 595, 709, 777, 814, 858
TseFI GTSAC 1 cut(s) 479
TseI GCWGC 5 cut(s) 188, 335, 682, 952, 1090
Tsp45I GTSAC 1 cut(s) 479
TspDTI ATGAA 4 cut(s) 388, 464, 525, 619
TspGWI ACGGA 2 cut(s) 359, 467
TspRI CASTG 5 cut(s) 595, 709, 777, 814, 858
Van91I CCANNNNNTGG 2 cut(s) 866, 881
Vha464I CTTAAG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 857
XapI RAATTY 2 cut(s) 395, 1045
XceI RCATGY 1 cut(s) 689
XmnI GAANNNNTTC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.