Rorug05G0516400
MADS Family

transcription regulatory region sequence-specific DNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0009469
Physical Location & Seq
Forward (+)
70089656 .. 70093903
4248 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0516400.1

Sequence Viewer

Length: 534 bp
ATGGACCTCCACACTCCCCATCCAACCACCCCTGATTGCCACGTGGTCGCATTGCCCTTCCCCGGCCGCGGCCACATCAACCCCATGATGTGTCTCTGCCACTCCATCGCCGCCATCTCCCCCGACACGACCGTCTCCTTCATCCTAACCGAAGAGTGGCTCGGCTTCATCGGATCCGACCCGAAACCCGACAACGTGCGATTCGTCACCATTCCCCAGGTCATTCCCTCGGAAATCGGTCGCGGCAAGGACTCTCCCAGCTTCTACGAGACTGTTCTGAAGAAGATCAGAGCGCCGGCGGAGGAGCTCCTAGATCGGCTCGAGCCGCCGGTGACGGCCTTGATCGCCGATACGTACTTGGTGTGGTCGGTTGAGATCGGGAATGTGAGGAATATTCCGGTGGCTTCTCTTTGGACTATGTCGGCTTCTGTCTATTCGGTGTTTCAATATTTTGATCTACTCGTTCAGAACCAGCATTTCCCTGTCAGTTTGGCGGGTTGTATTGGATTGAAGATTTCGTGGATTGAAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SRF-TF

Protein Analysis

177

Amino Acids

19.64

Weight (kDa)

5.12

Isoelectric Point (pI)

46.82

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 131
AccII CGCG 2 cut(s) 69, 243
AciI CCGC 7 cut(s) 67, 69, 111, 243, 299, 326, 494
AclWI GGATC 2 cut(s) 168, 181
AcoI YGGCCR 2 cut(s) 64, 70
AcuI CTGAAG 1 cut(s) 299
AcvI CACGTG 1 cut(s) 43
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 3 cut(s) 62, 68, 156
AgsI TTSAA 3 cut(s) 446, 511, 527
AjnI CCWGG 1 cut(s) 216
AluBI AGCT 2 cut(s) 261, 307
AluI AGCT 2 cut(s) 261, 307
Alw21I GWGCWC 1 cut(s) 309
Alw26I GTCTC 3 cut(s) 98, 139, 263
AlwI GGATC 2 cut(s) 168, 181
Ama87I CYCGRG 1 cut(s) 320
AoxI GGCC 3 cut(s) 64, 70, 336
AspLEI GCGC 1 cut(s) 295
AspS9I GGNCC 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 63
AsuHPI GGTGA 2 cut(s) 199, 343
AvaI CYCGRG 1 cut(s) 320
AvaII GGWCC 1 cut(s) 4
BamHI GGATCC 1 cut(s) 173
BanII GRGCYC 1 cut(s) 309
BbrPI CACGTG 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 309
BccI CCATC 3 cut(s) 27, 113, 122
BceAI ACGGC 1 cut(s) 351
BciT130I CCWGG 1 cut(s) 218
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 3 cut(s) 98, 139, 263
BfaI CTAG 1 cut(s) 311
BfoI RGCGCY 1 cut(s) 296
BisI GCNGC 5 cut(s) 67, 70, 111, 244, 326
BlsI GCNGC 5 cut(s) 68, 71, 112, 245, 327
Bme1390I CCNGG 2 cut(s) 63, 218
Bme18I GGWCC 1 cut(s) 4
BmeT110I CYCGRG 1 cut(s) 320
BmgT120I GGNCC 1 cut(s) 4
BmiI GGNNCC 1 cut(s) 175
BmrFI CCNGG 2 cut(s) 63, 218
BpuMI CCSGG 1 cut(s) 63
BsaAI YACGTR 2 cut(s) 43, 354
BsaJI CCNNGG 4 cut(s) 61, 67, 216, 228
BsaWI WCCGGW 1 cut(s) 397
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 68, 156
Bse118I RCCGGY 2 cut(s) 295, 328
Bse3DI GCAATG 1 cut(s) 50
BseBI CCWGG 1 cut(s) 218
BseDI CCNNGG 4 cut(s) 61, 67, 216, 228
BseGI GGATG 2 cut(s) 19, 141
BseLI CCNNNNNNNGG 3 cut(s) 62, 68, 156
BseMI GCAATG 1 cut(s) 50
BseRI GAGGAG 1 cut(s) 317
BseX3I CGGCCG 1 cut(s) 64
BseYI CCCAGC 1 cut(s) 257
Bsh1236I CGCG 2 cut(s) 69, 243
Bsh1285I CGRYCG 3 cut(s) 67, 132, 241
BshFI GGCC 3 cut(s) 66, 72, 338
BsiEI CGRYCG 3 cut(s) 67, 132, 241
BsiHKAI GWGCWC 1 cut(s) 309
BsiHKCI CYCGRG 1 cut(s) 320
BsiSI CCGG 4 cut(s) 63, 296, 329, 398
BslI CCNNNNNNNGG 3 cut(s) 62, 68, 156
BsmAI GTCTC 3 cut(s) 98, 139, 263
BsmBI CGTCTC 1 cut(s) 139
BsnI GGCC 3 cut(s) 66, 72, 338
BsoBI CYCGRG 1 cut(s) 320
Bsp1286I GDGCHC 1 cut(s) 309
Bsp143I GATC 6 cut(s) 173, 285, 313, 342, 375, 454
BspACI CCGC 7 cut(s) 67, 69, 111, 243, 299, 326, 494
BspANI GGCC 3 cut(s) 66, 72, 338
BspFNI CGCG 2 cut(s) 69, 243
BspLI GGNNCC 1 cut(s) 175
BspPI GGATC 2 cut(s) 168, 181
BsrDI GCAATG 1 cut(s) 50
BsrFI RCCGGY 2 cut(s) 295, 328
BssAI RCCGGY 2 cut(s) 295, 328
BssECI CCNNGG 4 cut(s) 61, 67, 216, 228
BssMI GATC 6 cut(s) 173, 285, 313, 342, 375, 454
Bst2UI CCWGG 1 cut(s) 218
Bst4CI ACNGT 2 cut(s) 133, 274
Bst6I CTCTTC 1 cut(s) 147
BstBAI YACGTR 2 cut(s) 43, 354
BstC8I GCNNGC 1 cut(s) 297
BstDSI CCRYGG 1 cut(s) 67
BstF5I GGATG 2 cut(s) 19, 141
BstFNI CGCG 2 cut(s) 69, 243
BstH2I RGCGCY 1 cut(s) 296
BstHHI GCGC 1 cut(s) 295
BstKTI GATC 6 cut(s) 176, 288, 316, 345, 378, 457
BstMAI GTCTC 3 cut(s) 98, 139, 263
BstMBI GATC 6 cut(s) 173, 285, 313, 342, 375, 454
BstMCI CGRYCG 3 cut(s) 67, 132, 241
BstMWI GCNNNNNNNGC 2 cut(s) 325, 344
BstNI CCWGG 1 cut(s) 218
BstSCI CCNGG 2 cut(s) 61, 216
BstSNI TACGTA 1 cut(s) 354
BstUI CGCG 2 cut(s) 69, 243
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BstZI CGGCCG 1 cut(s) 64
BsuRI GGCC 3 cut(s) 66, 72, 338
BtgI CCRYGG 1 cut(s) 67
BtgZI GCGATG 1 cut(s) 91
BtsCI GGATG 2 cut(s) 19, 141
Cac8I GCNNGC 1 cut(s) 297
CfoI GCGC 1 cut(s) 295
Cfr10I RCCGGY 2 cut(s) 295, 328
Cfr13I GGNCC 1 cut(s) 4
Cfr42I CCGCGG 1 cut(s) 70
Csp6I GTAC 1 cut(s) 355
CviAII CATG 1 cut(s) 85
CviQI GTAC 1 cut(s) 355
DpnI GATC 6 cut(s) 175, 287, 315, 344, 377, 456
DpnII GATC 6 cut(s) 173, 285, 313, 342, 375, 454
DrdI GACNNNNNNGTC 1 cut(s) 131
DseDI GACNNNNNNGTC 1 cut(s) 131
EaeI YGGCCR 2 cut(s) 64, 70
EagI CGGCCG 1 cut(s) 64
Eam1104I CTCTTC 1 cut(s) 147
EarI CTCTTC 1 cut(s) 147
EciI GGCGGA 1 cut(s) 314
Ecl136II GAGCTC 1 cut(s) 307
EclXI CGGCCG 1 cut(s) 64
Eco105I TACGTA 1 cut(s) 354
Eco24I GRGCYC 1 cut(s) 309
Eco47I GGWCC 1 cut(s) 4
Eco52I CGGCCG 1 cut(s) 64
Eco53kI GAGCTC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 299
Eco72I CACGTG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 320
EcoICRI GAGCTC 1 cut(s) 307
EcoRII CCWGG 1 cut(s) 216
EcoT38I GRGCYC 1 cut(s) 309
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 1 cut(s) 88
FaiI YATR 2 cut(s) 86, 419
FatI CATG 1 cut(s) 84
FauI CCCGC 1 cut(s) 487
Fnu4HI GCNGC 5 cut(s) 67, 70, 111, 244, 326
FokI GGATG 2 cut(s) 6, 128
FriOI GRGCYC 1 cut(s) 309
Fsp4HI GCNGC 5 cut(s) 67, 70, 111, 244, 326
FspBI CTAG 1 cut(s) 311
GlaI GCGC 1 cut(s) 294
GluI GCNGC 5 cut(s) 67, 70, 111, 244, 326
GsaI CCCAGC 1 cut(s) 261
HaeII RGCGCY 1 cut(s) 296
HaeIII GGCC 3 cut(s) 66, 72, 338
HapII CCGG 4 cut(s) 63, 296, 329, 398
HhaI GCGC 1 cut(s) 295
Hin1II CATG 1 cut(s) 88
Hin6I GCGC 1 cut(s) 293
HinP1I GCGC 1 cut(s) 293
HinfI GANTC 2 cut(s) 201, 251
HpaII CCGG 4 cut(s) 63, 296, 329, 398
HphI GGTGA 2 cut(s) 199, 343
Hpy188I TCNGA 6 cut(s) 173, 178, 232, 279, 290, 468
Hpy188III TCNNGA 1 cut(s) 379
HpyAV CCTTC 2 cut(s) 67, 148
HpyCH4III ACNGT 2 cut(s) 133, 274
HpyCH4IV ACGT 3 cut(s) 42, 195, 353
HpyF10VI GCNNNNNNNGC 2 cut(s) 325, 344
HpySE526I ACGT 3 cut(s) 42, 195, 353
Hsp92II CATG 1 cut(s) 88
HspAI GCGC 1 cut(s) 293
KroI GCCGGC 1 cut(s) 295
KroNI GCCGGC 1 cut(s) 297
KspI CCGCGG 1 cut(s) 70
Kzo9I GATC 6 cut(s) 173, 285, 313, 342, 375, 454
LmnI GCTCC 2 cut(s) 304, 312
MaeI CTAG 1 cut(s) 311
MaeII ACGT 3 cut(s) 42, 195, 353
MaeIII GTNAC 2 cut(s) 205, 331
MalI GATC 6 cut(s) 175, 287, 315, 344, 377, 456
MboI GATC 6 cut(s) 173, 285, 313, 342, 375, 454
MboII GAAGA 4 cut(s) 164, 292, 295, 523
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 309
MlyI GAGTC 1 cut(s) 245
MmeI TCCRAC 2 cut(s) 47, 201
MnlI CCTC 4 cut(s) 17, 238, 295, 381
MreI CGCCGGCG 1 cut(s) 295
MroNI GCCGGC 1 cut(s) 295
MspA1I CMGCKG 1 cut(s) 69
MspI CCGG 4 cut(s) 63, 296, 329, 398
MspR9I CCNGG 2 cut(s) 63, 218
MvaI CCWGG 1 cut(s) 218
MvnI CGCG 2 cut(s) 69, 243
MwoI GCNNNNNNNGC 2 cut(s) 325, 344
NaeI GCCGGC 1 cut(s) 297
NciI CCSGG 1 cut(s) 63
NdeII GATC 6 cut(s) 173, 285, 313, 342, 375, 454
NgoMIV GCCGGC 1 cut(s) 295
NlaIII CATG 1 cut(s) 88
NlaIV GGNNCC 1 cut(s) 175
NmeAIII GCCGAG 1 cut(s) 141
NmuCI GTSAC 2 cut(s) 205, 331
PaeR7I CTCGAG 1 cut(s) 320
PcsI WCGNNNNNNNCGW 1 cut(s) 201
PdiI GCCGGC 1 cut(s) 297
PfeI GAWTC 1 cut(s) 201
PflFI GACNNNGTC 1 cut(s) 418
PkrI GCNGC 5 cut(s) 68, 71, 112, 245, 327
PleI GAGTC 1 cut(s) 245
PmaCI CACGTG 1 cut(s) 43
PmlI CACGTG 1 cut(s) 43
PpsI GAGTC 1 cut(s) 245
Ppu21I YACGTR 2 cut(s) 43, 354
Psp124BI GAGCTC 1 cut(s) 309
Psp6I CCWGG 1 cut(s) 216
PspCI CACGTG 1 cut(s) 43
PspFI CCCAGC 1 cut(s) 257
PspGI CCWGG 1 cut(s) 216
PspN4I GGNNCC 1 cut(s) 175
PspPI GGNCC 1 cut(s) 4
PspXI VCTCGAGB 1 cut(s) 320
PsuI RGATCY 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 418
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SacI GAGCTC 1 cut(s) 309
SacII CCGCGG 1 cut(s) 70
SatI GCNGC 5 cut(s) 67, 70, 111, 244, 326
Sau3AI GATC 6 cut(s) 173, 285, 313, 342, 375, 454
Sau96I GGNCC 1 cut(s) 4
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 63, 218
SduI GDGCHC 1 cut(s) 309
SetI ASST 7 cut(s) 9, 45, 198, 222, 263, 309, 356
Sfr274I CTCGAG 1 cut(s) 320
Sfr303I CCGCGG 1 cut(s) 70
SgrAI CRCCGGYG 2 cut(s) 295, 328
SgrBI CCGCGG 1 cut(s) 70
SinI GGWCC 1 cut(s) 4
SlaI CTCGAG 1 cut(s) 320
SmlI CTYRAG 1 cut(s) 320
SmoI CTYRAG 1 cut(s) 320
SnaBI TACGTA 1 cut(s) 354
SsiI CCGC 7 cut(s) 67, 69, 111, 243, 299, 326, 494
SspI AATATT 2 cut(s) 394, 449
SspMI CTAG 1 cut(s) 311
SstI GAGCTC 1 cut(s) 309
StyD4I CCNGG 2 cut(s) 61, 216
TaaI ACNGT 2 cut(s) 133, 274
TaiI ACGT 3 cut(s) 45, 198, 356
TaqI TCGA 1 cut(s) 321
TaqII GACCGA 1 cut(s) 227
TauI GCSGC 5 cut(s) 69, 72, 113, 246, 328
TfiI GAWTC 1 cut(s) 201
TseFI GTSAC 2 cut(s) 205, 331
Tsp45I GTSAC 2 cut(s) 205, 331
TspDTI ATGAA 2 cut(s) 130, 157
Tth111I GACNNNGTC 1 cut(s) 418
VpaK11BI GGWCC 1 cut(s) 4
XhoI CTCGAG 1 cut(s) 320
XspI CTAG 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.