Rh6CG027600

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6C
OG0009469
Physical Location & Seq
Reverse (-)
2431560 .. 2456599
25040 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6CG027600.1

Sequence Viewer

Length: 1107 bp
ATGGAGACGAATCCAAAGAAGACTGGCGCTAGTGGGGGAGAGGTTCTAGACGGTTCGGATATAATGGAGTTGGTTGAGAACAAAGAGGTGTTCGGCCATTTCGTGGACCATAAGTTTCAGGAGCTGGATAGAGACAGAGATGGTCAGCTTTCTGTGAACGAGCTTAGGCCTGCTGTTGCTGATATTGGTGCTGCTCTTGGCTTACCTGTTCAGGGCGCTTCCCCTGACTCTGACCACATCTACTCTGAGGTATTGAATGAATTCACTCATGGCAAACAAGAAAAAGTGAACAAGGTCGAATTCAAAGAAGTTCTTTCTGATATTCTACTAGGCATGGCTGCTGGTTTGAAGCGAGACCCTATTGTGATTCTCCGCATTGATGGAGAAGACCTCCTAGAATACATCAATGGACCAACTTTTGAAGCAGAAATGGTGGCTGCACACTCTCTGATAGAGTCAGCTGCTGGAACCCTCCAAGACTGTATTATCAAAGCTTTTGAACTGCTCACAGTTGAACAAGGAATGCCCCCTTCATCAGATCCTTGGGTTGTGAGCAATATTGTGGAACCTGCTCTGCAGTCATGCGCTGGCCCTGACTGGAACAAGCCTGTCTCTCAAGAAACATTCCTAGTTGAATTCAAGAAAGTGGCCGAGTATGTGGCTCAACTTCTTAGGGAGCAGCATGTGATTGTAGCCCACAGCGAAAATACCTTTGATGGAAGTGGCATTAAGAGATTATTATCCAACAAGTTTGAATTAGACAAGACATTGAATGCAGCTATTGAGACTGCGCCAAAAGATCGCACTGGGAAAATATCAAAGGAGTATTTGCGTGTTGCGCTGGATGTGGTGGCTCCATCAGCTGGTCTTCCGCCTCTTGGTGCAGTTGAGCAGATGGACAAGATTGTTTTGGATGTGTTCAACCTGACGAATGCAGATGATGCAAAGCTGCTTAAAGAAGACGAGTTCAAGAAGATATTGACTGAAATCCTGGGGACTATCATGTTGCAATTGGAGGGCAGTCCCATCTCTGTTTATTCTAATTCAGTCGTGCACGAGCCCCTTGCCTCTTCTTCGACACTGTTGCAAGTACAGCCATCTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

368

Amino Acids

40.11

Weight (kDa)

4.65

Isoelectric Point (pI)

42.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 38 - 105 3.9e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccB7I CCANNNNNTGG 2 cut(s) 103, 863
AciI CCGC 2 cut(s) 373, 872
AclWI GGATC 1 cut(s) 533
AcoI YGGCCR 2 cut(s) 94, 648
AcsI RAATTY 3 cut(s) 260, 299, 635
AfaI GTAC 1 cut(s) 1092
AfiI CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
AjnI CCWGG 1 cut(s) 990
AluBI AGCT 8 cut(s) 124, 148, 163, 461, 494, 779, 863, 949
AluI AGCT 8 cut(s) 124, 148, 163, 461, 494, 779, 863, 949
Alw21I GWGCWC 1 cut(s) 1056
Alw26I GTCTC 4 cut(s) 126, 348, 616, 779
Alw44I GTGCAC 1 cut(s) 1052
AlwI GGATC 1 cut(s) 533
AlwNI CAGNNNCTG 2 cut(s) 124, 464
AoxI GGCC 4 cut(s) 94, 167, 589, 648
ApaLI GTGCAC 1 cut(s) 1052
ApeKI GCWGC 7 cut(s) 191, 338, 437, 461, 679, 776, 949
ApoI RAATTY 3 cut(s) 260, 299, 635
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 5 cut(s) 29, 218, 587, 793, 841
AspS9I GGNCC 3 cut(s) 106, 410, 590
AvaII GGWCC 2 cut(s) 106, 410
BaeGI GKGCMC 1 cut(s) 1056
BanII GRGCYC 1 cut(s) 1062
BauI CACGAG 1 cut(s) 1055
BbsI GAAGAC 4 cut(s) 26, 393, 860, 966
Bbv12I GWGCWC 1 cut(s) 1056
BbvI GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
BccI CCATC 7 cut(s) 134, 374, 710, 865, 889, 1034, 1105
BcgI CGANNNNNNTGC 4 cut(s) 1046, 1066, 1080, 1100
BciT130I CCWGG 1 cut(s) 992
BcoDI GTCTC 4 cut(s) 126, 348, 616, 779
BfaI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
BfmI CTRYAG 1 cut(s) 575
BfoI RGCGCY 2 cut(s) 30, 219
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 7 cut(s) 192, 339, 438, 462, 680, 777, 950
BlsI GCNGC 7 cut(s) 193, 340, 439, 463, 681, 778, 951
Bme1390I CCNGG 1 cut(s) 992
Bme18I GGWCC 2 cut(s) 106, 410
BmgT120I GGNCC 3 cut(s) 106, 410, 590
BmiI GGNNCC 3 cut(s) 469, 567, 855
BmrFI CCNGG 1 cut(s) 992
BmrI ACTGGG 1 cut(s) 816
BmsI GCATC 1 cut(s) 931
BmuI ACTGGG 1 cut(s) 816
BpiI GAAGAC 4 cut(s) 26, 393, 860, 966
BplI GAGNNNNNCTC 2 cut(s) 375, 407
Bpu10I CCTNAGC 1 cut(s) 164
BpuEI CTTGAG 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 739
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 542, 991
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
Bse1I ACTGG 3 cut(s) 28, 602, 811
Bse8I GATNNNNATC 1 cut(s) 739
BseBI CCWGG 1 cut(s) 992
BseDI CCNNGG 2 cut(s) 542, 991
BseGI GGATG 2 cut(s) 850, 919
BseJI GATNNNNATC 1 cut(s) 739
BseLI CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 28, 602, 811
BseSI GKGCMC 1 cut(s) 1056
BseXI GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
BsgI GTGCAG 2 cut(s) 423, 903
BshFI GGCC 4 cut(s) 96, 169, 591, 650
BsiHKAI GWGCWC 1 cut(s) 1056
BslFI GGGAC 2 cut(s) 1008, 1009
BslI CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
BsmAI GTCTC 4 cut(s) 126, 348, 616, 779
BsmFI GGGAC 2 cut(s) 1008, 1009
BsmI GAATGC 3 cut(s) 528, 778, 937
BsnI GGCC 4 cut(s) 96, 169, 591, 650
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 2 cut(s) 1056, 1062
Bsp143I GATC 2 cut(s) 538, 799
BspACI CCGC 2 cut(s) 373, 872
BspANI GGCC 4 cut(s) 96, 169, 591, 650
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 3 cut(s) 469, 567, 855
BspMAI CTGCAG 1 cut(s) 579
BspMI ACCTGC 1 cut(s) 577
BspPI GGATC 1 cut(s) 533
BspTNI GGTCTC 1 cut(s) 348
BsrI ACTGG 3 cut(s) 28, 602, 811
BssECI CCNNGG 2 cut(s) 542, 991
BssMI GATC 2 cut(s) 538, 799
BssSI CACGAG 1 cut(s) 1055
BssT1I CCWWGG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 1055
Bst2UI CCWGG 1 cut(s) 992
Bst4CI ACNGT 4 cut(s) 53, 482, 511, 1083
Bst6I CTCTTC 1 cut(s) 1075
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 2 cut(s) 171, 589
BstDEI CTNAG 3 cut(s) 164, 246, 671
BstF5I GGATG 2 cut(s) 850, 919
BstH2I RGCGCY 2 cut(s) 30, 219
BstHHI GCGC 5 cut(s) 29, 218, 587, 793, 841
BstKTI GATC 2 cut(s) 541, 802
BstMAI GTCTC 4 cut(s) 126, 348, 616, 779
BstMBI GATC 2 cut(s) 538, 799
BstMWI GCNNNNNNNGC 4 cut(s) 838, 860, 941, 1093
BstNI CCWGG 1 cut(s) 992
BstNSI RCATGY 1 cut(s) 686
BstSCI CCNGG 1 cut(s) 990
BstSFI CTRYAG 1 cut(s) 575
BstSLI GKGCMC 1 cut(s) 1056
BstV1I GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
BstV2I GAAGAC 4 cut(s) 26, 393, 860, 966
BstX2I RGATCY 1 cut(s) 538
BstYI RGATCY 1 cut(s) 538
BsuRI GGCC 4 cut(s) 96, 169, 591, 650
BtsCI GGATG 2 cut(s) 850, 919
BtsIMutI CAGTG 2 cut(s) 804, 1079
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 171, 589
CaiI CAGNNNCTG 2 cut(s) 124, 464
CfoI GCGC 5 cut(s) 29, 218, 587, 793, 841
Cfr13I GGNCC 3 cut(s) 106, 410, 590
Csp6I GTAC 1 cut(s) 1091
CviAII CATG 5 cut(s) 269, 334, 582, 683, 1003
CviQI GTAC 1 cut(s) 1091
DdeI CTNAG 3 cut(s) 164, 246, 671
DpnI GATC 2 cut(s) 540, 801
DpnII GATC 2 cut(s) 538, 799
EaeI YGGCCR 2 cut(s) 94, 648
Eam1104I CTCTTC 1 cut(s) 1075
EarI CTCTTC 1 cut(s) 1075
EciI GGCGGA 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 542
Eco147I AGGCCT 1 cut(s) 169
Eco24I GRGCYC 1 cut(s) 1062
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 106, 410
EcoRI GAATTC 3 cut(s) 260, 299, 635
EcoRII CCWGG 1 cut(s) 990
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 1 cut(s) 1062
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 5 cut(s) 272, 337, 585, 686, 1006
FaiI YATR 8 cut(s) 62, 111, 270, 335, 583, 657, 684, 1004
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FaqI GGGAC 2 cut(s) 1008, 1009
FatI CATG 5 cut(s) 268, 333, 581, 682, 1002
Fnu4HI GCNGC 7 cut(s) 192, 339, 438, 462, 680, 777, 950
FokI GGATG 2 cut(s) 857, 926
FriOI GRGCYC 1 cut(s) 1062
Fsp4HI GCNGC 7 cut(s) 192, 339, 438, 462, 680, 777, 950
FspBI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
GlaI GCGC 5 cut(s) 28, 217, 586, 792, 840
GluI GCNGC 7 cut(s) 192, 339, 438, 462, 680, 777, 950
HaeII RGCGCY 2 cut(s) 30, 219
HaeIII GGCC 4 cut(s) 96, 169, 591, 650
HhaI GCGC 5 cut(s) 29, 218, 587, 793, 841
Hin1II CATG 5 cut(s) 272, 337, 585, 686, 1006
Hin6I GCGC 5 cut(s) 27, 216, 585, 791, 839
HinP1I GCGC 5 cut(s) 27, 216, 585, 791, 839
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 4 cut(s) 10, 227, 367, 455
Hpy166II GTNNAC 4 cut(s) 106, 157, 289, 1054
Hpy188I TCNGA 6 cut(s) 58, 232, 247, 319, 450, 538
Hpy188III TCNNGA 5 cut(s) 47, 119, 617, 640, 970
Hpy8I GTNNAC 4 cut(s) 106, 157, 289, 1054
HpyAV CCTTC 1 cut(s) 540
HpyCH4III ACNGT 4 cut(s) 53, 482, 511, 1083
HpyCH4V TGCA 9 cut(s) 440, 577, 776, 884, 935, 944, 1009, 1054, 1087
HpyF10VI GCNNNNNNNGC 4 cut(s) 838, 860, 941, 1093
HpyF3I CTNAG 3 cut(s) 164, 246, 671
Hsp92II CATG 5 cut(s) 272, 337, 585, 686, 1006
HspAI GCGC 5 cut(s) 27, 216, 585, 791, 839
Kzo9I GATC 2 cut(s) 538, 799
LmnI GCTCC 3 cut(s) 121, 676, 859
Lsp1109I GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
LweI GCATC 1 cut(s) 931
MaeI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
MalI GATC 2 cut(s) 540, 801
MboI GATC 2 cut(s) 538, 799
MboII GAAGA 8 cut(s) 31, 398, 860, 971, 985, 1062, 1065, 1092
MfeI CAATTG 1 cut(s) 1010
MflI RGATCY 1 cut(s) 538
MhlI GDGCHC 2 cut(s) 1056, 1062
MluCI AATT 6 cut(s) 260, 299, 635, 755, 1010, 1042
MlyI GAGTC 2 cut(s) 221, 464
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 8 cut(s) 34, 79, 241, 401, 482, 885, 1009, 1078
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 2 cut(s) 729, 954
MspA1I CMGCKG 2 cut(s) 461, 863
MspR9I CCNGG 1 cut(s) 992
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 3 cut(s) 528, 778, 937
MvaI CCWGG 1 cut(s) 992
MwoI GCNNNNNNNGC 4 cut(s) 838, 860, 941, 1093
NdeII GATC 2 cut(s) 538, 799
NlaIII CATG 5 cut(s) 272, 337, 585, 686, 1006
NlaIV GGNNCC 3 cut(s) 469, 567, 855
NmeAIII GCCGAG 1 cut(s) 676
NspI RCATGY 1 cut(s) 686
PceI AGGCCT 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 99
PctI GAATGC 3 cut(s) 528, 778, 937
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 10, 367
PflMI CCANNNNNTGG 2 cut(s) 103, 863
PkrI GCNGC 7 cut(s) 193, 340, 439, 463, 681, 778, 951
PleI GAGTC 2 cut(s) 221, 463
PpsI GAGTC 2 cut(s) 221, 463
Psp6I CCWGG 1 cut(s) 990
PspGI CCWGG 1 cut(s) 990
PspN4I GGNNCC 3 cut(s) 469, 567, 855
PspPI GGNCC 3 cut(s) 106, 410, 590
PstI CTGCAG 1 cut(s) 579
PstNI CAGNNNCTG 2 cut(s) 124, 464
PsuI RGATCY 1 cut(s) 538
PvuII CAGCTG 2 cut(s) 461, 863
RsaI GTAC 1 cut(s) 1092
RsaNI GTAC 1 cut(s) 1091
SaqAI TTAA 2 cut(s) 729, 954
SatI GCNGC 7 cut(s) 192, 339, 438, 462, 680, 777, 950
Sau3AI GATC 2 cut(s) 538, 799
Sau96I GGNCC 3 cut(s) 106, 410, 590
SchI GAGTC 2 cut(s) 221, 464
ScrFI CCNGG 1 cut(s) 992
SduI GDGCHC 2 cut(s) 1056, 1062
SfaNI GCATC 1 cut(s) 931
SfcI CTRYAG 1 cut(s) 575
SinI GGWCC 2 cut(s) 106, 410
SmlI CTYRAG 1 cut(s) 615
SmoI CTYRAG 1 cut(s) 615
Sse9I AATT 6 cut(s) 260, 299, 635, 755, 1010, 1042
SseBI AGGCCT 1 cut(s) 169
SsiI CCGC 2 cut(s) 373, 872
SspI AATATT 1 cut(s) 559
SspMI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
StuI AGGCCT 1 cut(s) 169
StyD4I CCNGG 1 cut(s) 990
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 4 cut(s) 53, 482, 511, 1083
TaqI TCGA 2 cut(s) 297, 1076
TasI AATT 6 cut(s) 260, 299, 635, 755, 1010, 1042
TatI WGTACW 1 cut(s) 1090
TfiI GAWTC 2 cut(s) 10, 367
Tru1I TTAA 2 cut(s) 729, 954
Tru9I TTAA 2 cut(s) 729, 954
TscAI CASTG 2 cut(s) 811, 1086
TseI GCWGC 7 cut(s) 191, 338, 437, 461, 679, 776, 949
TspDTI ATGAA 2 cut(s) 273, 522
TspRI CASTG 2 cut(s) 811, 1086
Van91I CCANNNNNTGG 2 cut(s) 103, 863
VneI GTGCAC 1 cut(s) 1052
VpaK11BI GGWCC 2 cut(s) 106, 410
XapI RAATTY 3 cut(s) 260, 299, 635
XbaI TCTAGA 1 cut(s) 46
XceI RCATGY 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.