RLG00000015292

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009469
Physical Location & Seq
Forward (+)
64097161 .. 64100235
3075 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015292

Sequence Viewer

Length: 1107 bp
ATGGAGACGAATCCAAAGAAGACTGGCACTAGTGGGGGAGAGGTTCTAGACGGTTCGGATATAATGGAGTTGGTTGAGAACAAAGAGGTGTTCAGCCACTTCGTGGACCATAAGTTTCAGGAGCTGGATAGAGACAGAGATGGTCAGCTTTCTGTGAACGAGCTTCGGCCTGCTGTTGCTGATATTGGTGCTGCTCTTGGCTTACCTGTTCAGGGCTCTTCCCCTGACTCTGACCACATCTACTCTGAGGTATTGAATGAATTCACTCATGGCAAACAAGAAAAAGTGAGCAAGGTCGAGTTCAAAGAAGTTCTTTCTGATATTCTACTAGGCATGGCTGCTGGTTTGAAGCGAGACCCTATTGTGATTCTCCGCATTGATGGAGAAGACCTCCTAGAATACATCAATGGACCAACTTTTGAAGCAGAAATGGTGGCTGCACACTCACTGATAGAGTCAGCTGCTGGAACCCTCCAAGACTGTATTATCAAAGCTTTTGAACTGCTCACAGTTGAACAAGGGATGCCCCCTTCATCAGACCCTTGGGTTGTGAGCAATATTGTGGAACCTGCTCTGCAATCATGCGCTGGCCCTGACTGGAACAAGCCTGTCTCTCAAGAAACATTCCTAGTTGAATTCAAGAAAGTGGCCGAGTATGTGGCTCAACTTCTTAGGGAGCAGCATGTGATTGTAGCCCACAGTGAAAATACCTTTGATGGAAGTGGCATTAAGAGATTATTATCCAACAAGTTTGAATTAGACAAGACATTGAATGCAGCTATTGAGACTCTGCCAAAAGATCGCACTGGGAAAATATCGAAGGAGTATTTGCGTGTTGCGCTGGATGTGGTGGCTCCATCAGCTAGTCTTCCGCCTCTTGGTGCAGTTGAGCAGATGGACAAGATTGTTTTGGATGTGTTCAACCTGACGAATGCAGATGATGCAAAGCTGCTTAAAGAAGACGAGTTCAAGAAGATATTAACTGAAATCCTGGGAAGTATCATGTTGCAATTGGAGGGCAGTCCCATCTCTGTTTATTCTAATTCAGTCGTGCACGAGCCCCTTGCCGCTTCTTCGACACTGTTGCAAGTACGGCCATCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

369

Amino Acids

40.22

Weight (kDa)

4.68

Isoelectric Point (pI)

41.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 38 - 105 1.2e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccB7I CCANNNNNTGG 1 cut(s) 103
AciI CCGC 3 cut(s) 373, 872, 1068
AcoI YGGCCR 2 cut(s) 648, 1094
AcsI RAATTY 2 cut(s) 260, 635
AdeI CACNNNGTG 1 cut(s) 103
AfaI GTAC 1 cut(s) 1092
AfiI CCNNNNNNNGG 3 cut(s) 103, 212, 878
AhlI ACTAGT 1 cut(s) 29
AjnI CCWGG 1 cut(s) 990
AluBI AGCT 8 cut(s) 124, 148, 163, 461, 494, 779, 863, 949
AluI AGCT 8 cut(s) 124, 148, 163, 461, 494, 779, 863, 949
Alw21I GWGCWC 1 cut(s) 1056
Alw26I GTCTC 4 cut(s) 126, 348, 616, 779
Alw44I GTGCAC 1 cut(s) 1052
AlwNI CAGNNNCTG 2 cut(s) 124, 464
AoxI GGCC 4 cut(s) 167, 589, 648, 1094
ApaLI GTGCAC 1 cut(s) 1052
ApeKI GCWGC 7 cut(s) 191, 338, 437, 461, 679, 776, 949
ApoI RAATTY 2 cut(s) 260, 635
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 587, 841
AspS9I GGNCC 3 cut(s) 106, 410, 590
AvaII GGWCC 2 cut(s) 106, 410
BaeGI GKGCMC 1 cut(s) 1056
BanII GRGCYC 2 cut(s) 218, 1062
BauI CACGAG 1 cut(s) 1055
BbsI GAAGAC 4 cut(s) 26, 393, 860, 966
Bbv12I GWGCWC 1 cut(s) 1056
BbvI GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
BccI CCATC 7 cut(s) 134, 374, 710, 865, 889, 1034, 1105
BcgI CGANNNNNNTGC 4 cut(s) 1046, 1066, 1080, 1100
BciT130I CCWGG 1 cut(s) 992
BcoDI GTCTC 4 cut(s) 126, 348, 616, 779
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 7 cut(s) 30, 47, 329, 395, 629, 864, 1105
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 8 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068
BlsI GCNGC 8 cut(s) 193, 340, 439, 463, 681, 778, 951, 1069
Bme1390I CCNGG 1 cut(s) 992
Bme18I GGWCC 2 cut(s) 106, 410
BmgT120I GGNCC 3 cut(s) 106, 410, 590
BmiI GGNNCC 3 cut(s) 469, 567, 855
BmrFI CCNGG 1 cut(s) 992
BmrI ACTGGG 1 cut(s) 816
BmsI GCATC 2 cut(s) 513, 931
BmuI ACTGGG 1 cut(s) 816
BpiI GAAGAC 4 cut(s) 26, 393, 860, 966
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpuEI CTTGAG 1 cut(s) 600
BsaBI GATNNNNATC 1 cut(s) 739
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 542, 991
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 212, 878
Bse1I ACTGG 3 cut(s) 28, 602, 811
Bse8I GATNNNNATC 1 cut(s) 739
BseBI CCWGG 1 cut(s) 992
BseDI CCNNGG 2 cut(s) 542, 991
BseGI GGATG 3 cut(s) 528, 850, 919
BseJI GATNNNNATC 1 cut(s) 739
BseLI CCNNNNNNNGG 3 cut(s) 103, 212, 878
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 28, 602, 811
BseSI GKGCMC 1 cut(s) 1056
BseXI GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
BsgI GTGCAG 2 cut(s) 423, 903
BshFI GGCC 4 cut(s) 169, 591, 650, 1096
BsiHKAI GWGCWC 1 cut(s) 1056
BslFI GGGAC 1 cut(s) 1008
BslI CCNNNNNNNGG 3 cut(s) 103, 212, 878
BsmAI GTCTC 4 cut(s) 126, 348, 616, 779
BsmFI GGGAC 1 cut(s) 1008
BsmI GAATGC 2 cut(s) 778, 937
BsnI GGCC 4 cut(s) 169, 591, 650, 1096
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 3 cut(s) 218, 1056, 1062
Bsp143I GATC 1 cut(s) 799
BspACI CCGC 3 cut(s) 373, 872, 1068
BspANI GGCC 4 cut(s) 169, 591, 650, 1096
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 3 cut(s) 469, 567, 855
BspMI ACCTGC 1 cut(s) 577
BspQI GCTCTTC 1 cut(s) 223
BspTNI GGTCTC 1 cut(s) 348
BsrI ACTGG 3 cut(s) 28, 602, 811
BssECI CCNNGG 2 cut(s) 542, 991
BssMI GATC 1 cut(s) 799
BssSI CACGAG 1 cut(s) 1055
BssT1I CCWWGG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 1055
Bst2UI CCWGG 1 cut(s) 992
Bst4CI ACNGT 5 cut(s) 53, 482, 511, 701, 1083
Bst6I CTCTTC 1 cut(s) 223
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 2 cut(s) 171, 589
BstDEI CTNAG 2 cut(s) 246, 671
BstF5I GGATG 3 cut(s) 528, 850, 919
BstHHI GCGC 2 cut(s) 587, 841
BstKTI GATC 1 cut(s) 802
BstMAI GTCTC 4 cut(s) 126, 348, 616, 779
BstMBI GATC 1 cut(s) 799
BstMWI GCNNNNNNNGC 4 cut(s) 838, 860, 941, 1093
BstNI CCWGG 1 cut(s) 992
BstNSI RCATGY 1 cut(s) 686
BstSCI CCNGG 1 cut(s) 990
BstSLI GKGCMC 1 cut(s) 1056
BstV1I GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
BstV2I GAAGAC 4 cut(s) 26, 393, 860, 966
BsuRI GGCC 4 cut(s) 169, 591, 650, 1096
BtsCI GGATG 3 cut(s) 528, 850, 919
BtsIMutI CAGTG 4 cut(s) 446, 706, 804, 1079
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 171, 589
CaiI CAGNNNCTG 2 cut(s) 124, 464
CfoI GCGC 2 cut(s) 587, 841
Cfr13I GGNCC 3 cut(s) 106, 410, 590
Csp6I GTAC 1 cut(s) 1091
CviAII CATG 5 cut(s) 269, 334, 582, 683, 1003
CviQI GTAC 1 cut(s) 1091
DdeI CTNAG 2 cut(s) 246, 671
DpnI GATC 1 cut(s) 801
DpnII GATC 1 cut(s) 799
DraIII CACNNNGTG 1 cut(s) 103
EaeI YGGCCR 2 cut(s) 648, 1094
Eam1104I CTCTTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 223
EciI GGCGGA 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 2 cut(s) 218, 1062
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 106, 410
EcoRI GAATTC 2 cut(s) 260, 635
EcoRII CCWGG 1 cut(s) 990
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 2 cut(s) 218, 1062
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 5 cut(s) 272, 337, 585, 686, 1006
FaiI YATR 8 cut(s) 62, 111, 270, 335, 583, 657, 684, 1004
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FaqI GGGAC 1 cut(s) 1008
FatI CATG 5 cut(s) 268, 333, 581, 682, 1002
Fnu4HI GCNGC 8 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068
FokI GGATG 3 cut(s) 535, 857, 926
FriOI GRGCYC 2 cut(s) 218, 1062
Fsp4HI GCNGC 8 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068
FspBI CTAG 7 cut(s) 30, 47, 329, 395, 629, 864, 1105
GlaI GCGC 2 cut(s) 586, 840
GluI GCNGC 8 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068
HaeIII GGCC 4 cut(s) 169, 591, 650, 1096
HhaI GCGC 2 cut(s) 587, 841
Hin1II CATG 5 cut(s) 272, 337, 585, 686, 1006
Hin6I GCGC 2 cut(s) 585, 839
HinP1I GCGC 2 cut(s) 585, 839
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 5 cut(s) 10, 227, 367, 455, 787
Hpy166II GTNNAC 3 cut(s) 106, 157, 1054
Hpy188I TCNGA 5 cut(s) 58, 232, 247, 319, 538
Hpy188III TCNNGA 5 cut(s) 47, 119, 617, 640, 970
Hpy8I GTNNAC 3 cut(s) 106, 157, 1054
HpyAV CCTTC 2 cut(s) 540, 814
HpyCH4III ACNGT 5 cut(s) 53, 482, 511, 701, 1083
HpyCH4V TGCA 9 cut(s) 440, 577, 776, 884, 935, 944, 1009, 1054, 1087
HpyF10VI GCNNNNNNNGC 4 cut(s) 838, 860, 941, 1093
HpyF3I CTNAG 2 cut(s) 246, 671
Hsp92II CATG 5 cut(s) 272, 337, 585, 686, 1006
HspAI GCGC 2 cut(s) 585, 839
Kzo9I GATC 1 cut(s) 799
LguI GCTCTTC 1 cut(s) 223
LmnI GCTCC 3 cut(s) 121, 676, 859
Lsp1109I GCAGC 7 cut(s) 178, 325, 424, 448, 691, 788, 936
LweI GCATC 2 cut(s) 513, 931
MaeI CTAG 7 cut(s) 30, 47, 329, 395, 629, 864, 1105
MalI GATC 1 cut(s) 801
MboI GATC 1 cut(s) 799
MboII GAAGA 7 cut(s) 31, 210, 398, 860, 971, 985, 1065
MfeI CAATTG 1 cut(s) 1010
MhlI GDGCHC 3 cut(s) 218, 1056, 1062
MluCI AATT 5 cut(s) 260, 635, 755, 1010, 1042
MlyI GAGTC 3 cut(s) 221, 464, 781
MmeI TCCRAC 1 cut(s) 768
MnlI CCTC 7 cut(s) 34, 79, 241, 401, 482, 885, 1009
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 3 cut(s) 729, 954, 980
MspA1I CMGCKG 1 cut(s) 461
MspR9I CCNGG 1 cut(s) 992
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 2 cut(s) 778, 937
MvaI CCWGG 1 cut(s) 992
MwoI GCNNNNNNNGC 4 cut(s) 838, 860, 941, 1093
NdeII GATC 1 cut(s) 799
NlaIII CATG 5 cut(s) 272, 337, 585, 686, 1006
NlaIV GGNNCC 3 cut(s) 469, 567, 855
NmeAIII GCCGAG 1 cut(s) 676
NspI RCATGY 1 cut(s) 686
PciSI GCTCTTC 1 cut(s) 223
PctI GAATGC 2 cut(s) 778, 937
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 10, 367
PflMI CCANNNNNTGG 1 cut(s) 103
PkrI GCNGC 8 cut(s) 193, 340, 439, 463, 681, 778, 951, 1069
PleI GAGTC 3 cut(s) 221, 463, 781
PpsI GAGTC 3 cut(s) 221, 463, 781
Psp6I CCWGG 1 cut(s) 990
PspGI CCWGG 1 cut(s) 990
PspN4I GGNNCC 3 cut(s) 469, 567, 855
PspPI GGNCC 3 cut(s) 106, 410, 590
PstNI CAGNNNCTG 2 cut(s) 124, 464
PvuII CAGCTG 1 cut(s) 461
RsaI GTAC 1 cut(s) 1092
RsaNI GTAC 1 cut(s) 1091
SapI GCTCTTC 1 cut(s) 223
SaqAI TTAA 3 cut(s) 729, 954, 980
SatI GCNGC 8 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068
Sau3AI GATC 1 cut(s) 799
Sau96I GGNCC 3 cut(s) 106, 410, 590
SchI GAGTC 3 cut(s) 221, 464, 781
ScrFI CCNGG 1 cut(s) 992
SduI GDGCHC 3 cut(s) 218, 1056, 1062
SfaNI GCATC 2 cut(s) 513, 931
SinI GGWCC 2 cut(s) 106, 410
SmlI CTYRAG 1 cut(s) 615
SmoI CTYRAG 1 cut(s) 615
SpeI ACTAGT 1 cut(s) 29
Sse9I AATT 5 cut(s) 260, 635, 755, 1010, 1042
SsiI CCGC 3 cut(s) 373, 872, 1068
SspI AATATT 1 cut(s) 559
SspMI CTAG 7 cut(s) 30, 47, 329, 395, 629, 864, 1105
StyD4I CCNGG 1 cut(s) 990
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 5 cut(s) 53, 482, 511, 701, 1083
TaqI TCGA 3 cut(s) 297, 818, 1076
TasI AATT 5 cut(s) 260, 635, 755, 1010, 1042
TauI GCSGC 1 cut(s) 1070
TfiI GAWTC 2 cut(s) 10, 367
Tru1I TTAA 3 cut(s) 729, 954, 980
Tru9I TTAA 3 cut(s) 729, 954, 980
TscAI CASTG 4 cut(s) 453, 706, 811, 1086
TseI GCWGC 7 cut(s) 191, 338, 437, 461, 679, 776, 949
TspDTI ATGAA 2 cut(s) 273, 522
TspRI CASTG 4 cut(s) 453, 706, 811, 1086
Van91I CCANNNNNTGG 1 cut(s) 103
VneI GTGCAC 1 cut(s) 1052
VpaK11BI GGWCC 2 cut(s) 106, 410
XapI RAATTY 2 cut(s) 260, 635
XbaI TCTAGA 1 cut(s) 46
XceI RCATGY 1 cut(s) 686
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 7 cut(s) 30, 47, 329, 395, 629, 864, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.