Rw6G002630

EF-hand, calcium binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0009469
Physical Location & Seq
Reverse (-)
4199198 .. 4202250
3053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G002630.1

Sequence Viewer

Length: 1107 bp
ATGGAGACGAATCCAAAGAAGACTGGCACTAGTGGGGGAGAGGTTCTAGACGGTTCGGATATAATGGAGTTGGTTGAGAACAAAGAGGTGTTCAGCCACTTCGTGGACCATAAGTTTGAGGAGCTGGATAGAGACAGAGATGGTCAGCTTTCTGTGAACGAGCTTCAGCCTGCTGTTGCTGATATTGGTGCTGCTCTTGGCTTACCTGTTCAGGGCTCCTCCCCTGACTCTGACCACATCTACTCTGAGGTATTGAATGAATTCACTCATGGCAAACAAGAAAAAGTGAGCAAGGTCGAGTTCAAAGAAGTTCTTTCTGATATTCTACTAGGCATGGCTGCTGGTTTGAAGCGAGACCCTATTGTGATTCTCCGCATTGATGGAGAAGACCTCCTAGAATACATCAATGGACCAACTTTTGAAGCAGAAATGGTGGCTGCACACTCTCTGATAGAGTCAGCTGCTGGAACCCTCCAAGACTGTATTATCAAAGCTTTTGAACTGCTCACAGTTGAACAAGGGATGCCCCCTTCATCAGACCCTTGGGTTGTGAGCAATATTGTGGAACCTGCTCTGCAATCATGCGCTGGCCCTGACTGGAACAAGCCTGTCTCTCAAGAAACATTCCTAGTTGAATTCAAGAAAGTGGCCGAGCATGTGGCTCAACTTCTTAGGGAGCAGCATGTGATTGTAGCCCACAGTGAAAATACCTTTGATGGAAGTGGCATTAAGAGATTATTAACCAACAAGTTTGAATTAGACAAGACATTGAATGCAGCTATTGAGACTCTGCCAAAAGATCGCACTGGGAAAATATCGAAGGAGTATTTGCGTGTTGCGCTGGATGCCGTGGCTCCATCAGCTGGTCTTCCGCCTCTTGGTGCAGTTGAGCAGATGGACAAGATTGTTTTGGATGTGTTCAACCTGACGAATGCAGATGATGCAAAGCTGCTTAAAGAAGACGAGTTCAAGAAGATATTAACTGAAATCCTGGGGAGTATCATGTTGCAATTGGAGGGCAGTCCCATCTCTGTTTATTCTAATTCAGTCGTGCACGAGCCCCTTGCCGCTGCTTCGACATTGTTGCAAGTACAGCCACCTGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5 EF-hand_7

Protein Analysis

368

Amino Acids

40.08

Weight (kDa)

4.62

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 38 - 105 2e-07 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 577
AccB7I CCANNNNNTGG 2 cut(s) 103, 863
AciI CCGC 3 cut(s) 373, 872, 1068
AcoI YGGCCR 1 cut(s) 648
AcsI RAATTY 2 cut(s) 260, 635
AcuI CTGAAG 1 cut(s) 149
AdeI CACNNNGTG 1 cut(s) 103
AfaI GTAC 1 cut(s) 1092
AfiI CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
AhlI ACTAGT 1 cut(s) 29
AjnI CCWGG 1 cut(s) 990
AluBI AGCT 8 cut(s) 124, 148, 163, 461, 494, 779, 863, 949
AluI AGCT 8 cut(s) 124, 148, 163, 461, 494, 779, 863, 949
Alw21I GWGCWC 1 cut(s) 1056
Alw26I GTCTC 4 cut(s) 126, 348, 616, 779
Alw44I GTGCAC 1 cut(s) 1052
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 2 cut(s) 589, 648
ApaLI GTGCAC 1 cut(s) 1052
ApeKI GCWGC 8 cut(s) 191, 338, 437, 461, 679, 776, 949, 1070
ApoI RAATTY 2 cut(s) 260, 635
ArsI GACNNNNNNTTYG 2 cut(s) 98, 130
Asp700I GAANNNNTTC 1 cut(s) 260
AspLEI GCGC 2 cut(s) 587, 841
AspS9I GGNCC 3 cut(s) 106, 410, 590
AvaII GGWCC 2 cut(s) 106, 410
BaeGI GKGCMC 1 cut(s) 1056
BanII GRGCYC 2 cut(s) 218, 1062
BauI CACGAG 1 cut(s) 1055
BbsI GAAGAC 4 cut(s) 26, 393, 860, 966
Bbv12I GWGCWC 1 cut(s) 1056
BbvI GCAGC 8 cut(s) 178, 325, 424, 448, 691, 788, 936, 1057
BccI CCATC 6 cut(s) 134, 374, 710, 865, 889, 1034
BceAI ACGGC 1 cut(s) 833
BcgI CGANNNNNNTGC 4 cut(s) 1046, 1066, 1080, 1100
BciT130I CCWGG 1 cut(s) 992
BcoDI GTCTC 4 cut(s) 126, 348, 616, 779
BcuI ACTAGT 1 cut(s) 29
BfaI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
BfuAI ACCTGC 1 cut(s) 577
BisI GCNGC 9 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068, 1071
BlsI GCNGC 9 cut(s) 193, 340, 439, 463, 681, 778, 951, 1069, 1072
Bme1390I CCNGG 1 cut(s) 992
Bme18I GGWCC 2 cut(s) 106, 410
BmgT120I GGNCC 3 cut(s) 106, 410, 590
BmiI GGNNCC 4 cut(s) 217, 469, 567, 855
BmrFI CCNGG 1 cut(s) 992
BmrI ACTGGG 1 cut(s) 816
BmsI GCATC 3 cut(s) 513, 835, 931
BmuI ACTGGG 1 cut(s) 816
BpiI GAAGAC 4 cut(s) 26, 393, 860, 966
BplI GAGNNNNNCTC 2 cut(s) 375, 407
BpuEI CTTGAG 1 cut(s) 600
BsaI GGTCTC 1 cut(s) 348
BsaJI CCNNGG 3 cut(s) 542, 849, 991
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
Bse1I ACTGG 3 cut(s) 28, 602, 811
BseBI CCWGG 1 cut(s) 992
BseDI CCNNGG 3 cut(s) 542, 849, 991
BseGI GGATG 3 cut(s) 528, 850, 919
BseLI CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
BseMII CTCAG 1 cut(s) 237
BseNI ACTGG 3 cut(s) 28, 602, 811
BseRI GAGGAG 2 cut(s) 134, 208
BseSI GKGCMC 1 cut(s) 1056
BseXI GCAGC 8 cut(s) 178, 325, 424, 448, 691, 788, 936, 1057
BsgI GTGCAG 2 cut(s) 423, 903
BshFI GGCC 2 cut(s) 591, 650
BsiHKAI GWGCWC 1 cut(s) 1056
BslFI GGGAC 1 cut(s) 1008
BslI CCNNNNNNNGG 4 cut(s) 103, 212, 863, 878
BsmAI GTCTC 4 cut(s) 126, 348, 616, 779
BsmFI GGGAC 1 cut(s) 1008
BsmI GAATGC 2 cut(s) 778, 937
BsnI GGCC 2 cut(s) 591, 650
Bso31I GGTCTC 1 cut(s) 348
Bsp1286I GDGCHC 3 cut(s) 218, 1056, 1062
Bsp143I GATC 1 cut(s) 799
BspACI CCGC 3 cut(s) 373, 872, 1068
BspANI GGCC 2 cut(s) 591, 650
BspCNI CTCAG 1 cut(s) 238
BspLI GGNNCC 4 cut(s) 217, 469, 567, 855
BspMI ACCTGC 1 cut(s) 577
BspTNI GGTCTC 1 cut(s) 348
BsrI ACTGG 3 cut(s) 28, 602, 811
BssECI CCNNGG 3 cut(s) 542, 849, 991
BssMI GATC 1 cut(s) 799
BssSI CACGAG 1 cut(s) 1055
BssT1I CCWWGG 1 cut(s) 542
Bst2BI CACGAG 1 cut(s) 1055
Bst2UI CCWGG 1 cut(s) 992
Bst4CI ACNGT 4 cut(s) 53, 482, 511, 701
BstAPI GCANNNNNTGC 1 cut(s) 941
BstC8I GCNNGC 2 cut(s) 171, 589
BstDEI CTNAG 2 cut(s) 246, 671
BstDSI CCRYGG 1 cut(s) 849
BstF5I GGATG 3 cut(s) 528, 850, 919
BstHHI GCGC 2 cut(s) 587, 841
BstKTI GATC 1 cut(s) 802
BstMAI GTCTC 4 cut(s) 126, 348, 616, 779
BstMBI GATC 1 cut(s) 799
BstMWI GCNNNNNNNGC 5 cut(s) 838, 845, 860, 941, 1093
BstNI CCWGG 1 cut(s) 992
BstNSI RCATGY 2 cut(s) 659, 686
BstSCI CCNGG 1 cut(s) 990
BstSLI GKGCMC 1 cut(s) 1056
BstV1I GCAGC 8 cut(s) 178, 325, 424, 448, 691, 788, 936, 1057
BstV2I GAAGAC 4 cut(s) 26, 393, 860, 966
BsuRI GGCC 2 cut(s) 591, 650
BtgI CCRYGG 1 cut(s) 849
BtsCI GGATG 3 cut(s) 528, 850, 919
BtsIMutI CAGTG 2 cut(s) 706, 804
BveI ACCTGC 1 cut(s) 577
Cac8I GCNNGC 2 cut(s) 171, 589
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 2 cut(s) 587, 841
Cfr13I GGNCC 3 cut(s) 106, 410, 590
Csp6I GTAC 1 cut(s) 1091
CviAII CATG 6 cut(s) 269, 334, 582, 656, 683, 1003
CviQI GTAC 1 cut(s) 1091
DdeI CTNAG 2 cut(s) 246, 671
DpnI GATC 1 cut(s) 801
DpnII GATC 1 cut(s) 799
DraIII CACNNNGTG 1 cut(s) 103
EaeI YGGCCR 1 cut(s) 648
EciI GGCGGA 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 542
Eco24I GRGCYC 2 cut(s) 218, 1062
Eco31I GGTCTC 1 cut(s) 348
Eco47I GGWCC 2 cut(s) 106, 410
Eco57I CTGAAG 1 cut(s) 149
EcoRI GAATTC 2 cut(s) 260, 635
EcoRII CCWGG 1 cut(s) 990
EcoT14I CCWWGG 1 cut(s) 542
EcoT38I GRGCYC 2 cut(s) 218, 1062
ErhI CCWWGG 1 cut(s) 542
FaeI CATG 6 cut(s) 272, 337, 585, 659, 686, 1006
FaiI YATR 8 cut(s) 62, 111, 270, 335, 583, 657, 684, 1004
FalI AAGNNNNNCTT 2 cut(s) 297, 329
FaqI GGGAC 1 cut(s) 1008
FatI CATG 6 cut(s) 268, 333, 581, 655, 682, 1002
Fnu4HI GCNGC 9 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068, 1071
FokI GGATG 3 cut(s) 535, 857, 926
FriOI GRGCYC 2 cut(s) 218, 1062
Fsp4HI GCNGC 9 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068, 1071
FspBI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
GlaI GCGC 2 cut(s) 586, 840
GluI GCNGC 9 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068, 1071
HaeIII GGCC 2 cut(s) 591, 650
HhaI GCGC 2 cut(s) 587, 841
Hin1II CATG 6 cut(s) 272, 337, 585, 659, 686, 1006
Hin6I GCGC 2 cut(s) 585, 839
HinP1I GCGC 2 cut(s) 585, 839
HindIII AAGCTT 1 cut(s) 492
HinfI GANTC 5 cut(s) 10, 227, 367, 455, 787
Hpy166II GTNNAC 3 cut(s) 106, 157, 1054
Hpy188I TCNGA 6 cut(s) 58, 232, 247, 319, 450, 538
Hpy188III TCNNGA 4 cut(s) 47, 617, 640, 970
Hpy8I GTNNAC 3 cut(s) 106, 157, 1054
HpyAV CCTTC 2 cut(s) 540, 814
HpyCH4III ACNGT 4 cut(s) 53, 482, 511, 701
HpyCH4V TGCA 9 cut(s) 440, 577, 776, 884, 935, 944, 1009, 1054, 1087
HpyF10VI GCNNNNNNNGC 5 cut(s) 838, 845, 860, 941, 1093
HpyF3I CTNAG 2 cut(s) 246, 671
Hsp92II CATG 6 cut(s) 272, 337, 585, 659, 686, 1006
HspAI GCGC 2 cut(s) 585, 839
Kzo9I GATC 1 cut(s) 799
LmnI GCTCC 4 cut(s) 121, 221, 676, 859
Lsp1109I GCAGC 8 cut(s) 178, 325, 424, 448, 691, 788, 936, 1057
LweI GCATC 3 cut(s) 513, 835, 931
MaeI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
MalI GATC 1 cut(s) 801
MboI GATC 1 cut(s) 799
MboII GAAGA 5 cut(s) 31, 398, 860, 971, 985
MfeI CAATTG 1 cut(s) 1010
MhlI GDGCHC 3 cut(s) 218, 1056, 1062
MluCI AATT 5 cut(s) 260, 635, 755, 1010, 1042
MlyI GAGTC 3 cut(s) 221, 464, 781
MnlI CCTC 9 cut(s) 34, 79, 112, 229, 241, 401, 482, 885, 1009
MroXI GAANNNNTTC 1 cut(s) 260
MseI TTAA 4 cut(s) 729, 740, 954, 980
MspA1I CMGCKG 3 cut(s) 461, 863, 1070
MspR9I CCNGG 1 cut(s) 992
MunI CAATTG 1 cut(s) 1010
Mva1269I GAATGC 2 cut(s) 778, 937
MvaI CCWGG 1 cut(s) 992
MwoI GCNNNNNNNGC 5 cut(s) 838, 845, 860, 941, 1093
NdeII GATC 1 cut(s) 799
NlaIII CATG 6 cut(s) 272, 337, 585, 659, 686, 1006
NlaIV GGNNCC 4 cut(s) 217, 469, 567, 855
NmeAIII GCCGAG 1 cut(s) 676
NspI RCATGY 2 cut(s) 659, 686
PctI GAATGC 2 cut(s) 778, 937
PdmI GAANNNNTTC 1 cut(s) 260
PfeI GAWTC 2 cut(s) 10, 367
PflMI CCANNNNNTGG 2 cut(s) 103, 863
PkrI GCNGC 9 cut(s) 193, 340, 439, 463, 681, 778, 951, 1069, 1072
PleI GAGTC 3 cut(s) 221, 463, 781
PpsI GAGTC 3 cut(s) 221, 463, 781
Psp6I CCWGG 1 cut(s) 990
PspGI CCWGG 1 cut(s) 990
PspN4I GGNNCC 4 cut(s) 217, 469, 567, 855
PspPI GGNCC 3 cut(s) 106, 410, 590
PstNI CAGNNNCTG 1 cut(s) 464
PvuII CAGCTG 2 cut(s) 461, 863
RsaI GTAC 1 cut(s) 1092
RsaNI GTAC 1 cut(s) 1091
SaqAI TTAA 4 cut(s) 729, 740, 954, 980
SatI GCNGC 9 cut(s) 192, 339, 438, 462, 680, 777, 950, 1068, 1071
Sau3AI GATC 1 cut(s) 799
Sau96I GGNCC 3 cut(s) 106, 410, 590
SchI GAGTC 3 cut(s) 221, 464, 781
ScrFI CCNGG 1 cut(s) 992
SduI GDGCHC 3 cut(s) 218, 1056, 1062
SfaNI GCATC 3 cut(s) 513, 835, 931
SinI GGWCC 2 cut(s) 106, 410
SmlI CTYRAG 1 cut(s) 615
SmoI CTYRAG 1 cut(s) 615
SpeI ACTAGT 1 cut(s) 29
Sse9I AATT 5 cut(s) 260, 635, 755, 1010, 1042
SsiI CCGC 3 cut(s) 373, 872, 1068
SspI AATATT 1 cut(s) 559
SspMI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
StyD4I CCNGG 1 cut(s) 990
StyI CCWWGG 1 cut(s) 542
TaaI ACNGT 4 cut(s) 53, 482, 511, 701
TaqI TCGA 3 cut(s) 297, 818, 1076
TasI AATT 5 cut(s) 260, 635, 755, 1010, 1042
TatI WGTACW 1 cut(s) 1090
TauI GCSGC 1 cut(s) 1070
TfiI GAWTC 2 cut(s) 10, 367
Tru1I TTAA 4 cut(s) 729, 740, 954, 980
Tru9I TTAA 4 cut(s) 729, 740, 954, 980
TscAI CASTG 2 cut(s) 706, 811
TseI GCWGC 8 cut(s) 191, 338, 437, 461, 679, 776, 949, 1070
TspDTI ATGAA 2 cut(s) 273, 522
TspRI CASTG 2 cut(s) 706, 811
Van91I CCANNNNNTGG 2 cut(s) 103, 863
VneI GTGCAC 1 cut(s) 1052
VpaK11BI GGWCC 2 cut(s) 106, 410
XapI RAATTY 2 cut(s) 260, 635
XbaI TCTAGA 1 cut(s) 46
XceI RCATGY 2 cut(s) 659, 686
XmnI GAANNNNTTC 1 cut(s) 260
XspI CTAG 6 cut(s) 30, 47, 329, 395, 629, 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.