Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0007674

Physical Location & Seq

Genomic Coordinates Forward (+)

28335239 .. 28339708

Gene Structure 4470 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1152500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3348 bp

ATGGCCAACTTCTTCATCTTCCTCCTGCTCTTCTTATCCTCTCCACTCACCGCATCTCCAAGGCCAAACCATAGAGTCCTCCACCAGCCATTTCTGCCGCAGGAATCTTCTTCGCCTCCATCTCCCTCACCTCCTTCCCCACCTCCGAACCCAACTCCCCCAGTTTCCAGTTCCAACTCTCCCCCTCCCAATTCCCCTTTTTTCCCATCCTTACCCTCCCCTCCGCCTACCCCTCCCTCCCCAACCACTGCCTTCGCCTCCTTCCCCGCCAACATCTCCTCCCTCATTGCCCCCCAGCCCACCAAAAACTCCAGCGCCCACAAGCACCTCGCCCTCACTATCGCCGCCGTGCTCTCCGCCGTCATGGTCGTTGCCGTCGCCGCATTCTTCCTCTACCGTCGCAAACACCACAACAGCCGCAACTTCCCGGACAACGAGGACGAAAAGTCGTATGTTAGATCCGAGCACAGCAGCAGGCTATTTCAGGTGCCCAATCTCAGCCACTCCTTCAGTGGTACACACAAGGTCCGAACCGGCTCTGCCAACAGCTCCGAGTTCCTCTACCTGGGCACAGTGGTCAACTCGCGCAGACTCGAGGAACGAGTTGACCCGCGCGGGAGCGTTGGTCGTAGGGCCGAAAAAGAAACTCGGAAAGTCGACTCGCCGGACCTCCAGCCGCTTCCGCCGCTTGTTCGGCAAACCTCGAGGCTGAGCCGTTGGGAGAATGCCGAACCGGTTCTGACCCATGACCGAGATGACGAGGAAGAGGAAGAGGAAGAAGAGTTCTACTCTCCCAGAGGGTCTTCGGGCGATCGCGACAGCTCCAATGGAAACGGATCCGGGTCCAGGAGAGTATTCGCGGCGGTTGCGGACGGCGTATTTGATGAAAGAGGCAGCGAAACGACGTCGTGTTCGTGCTCGTCTTCCGAGTTGGGTTCACCGTCTCGGTCACACTCAATCAGTCTCTCGCCTCCGGTGAGTTTAAGTCCCAACAGAAGATCAGAAGAGCCCAAACCGCCGCAACCCTCAGCCATGCATTACACGGCGCCGTTTGCTGATGTCAATGTACGGTCGCCGTCGTTGACTCCAATGTCTTCGCCGGAGAGAGTTGTTGATGATTTCCCATCTGCATCGGACCGAAACGGAGGGTCTCCGTCGATGTCCTCGATATCTTCGTCACCGGAGAGAGGATTGGAGAAGACCCCTGATGTACCGGCGAAAGTTTCGGTCGTTCCGGATCAAAGTTCGCCGATTTCTTCACCGGAGAGAGATTTCGGAAGCAACCAACATGCATCATCGAAAGTTTCGGTGGTTTCTGATCAAAGTTCGCCGATTTCCTCACTGGAATTAAATTTCAGAAACAAACCAGATGCGTTACCGAAAATTTCGGCCTTTTCAGATCGGAACGATCAATCCCCTTCACCATCTTTACATTGTTCATTGTCACCAGAGAGAGAATCGAACGGTTCTGATTCAAGAGCCAAGTCCTTTTCACCTTCTATGTCACCACTGAGAGGTTTGGAGAAGAGCTCAGCTGCCTCACCAAGAATTTCAAATGCTTCGGATCGAGCATTTATTCATTTGGATCCTAAAATGCAGTCTCTTTCATCATCTTCATCGTCTTCGTTGTCGAATTCACCGGAGAGAGAGTTTAGTGGTGGTTTAAAGCAGCCTCTGACTTCAACCGAGAAAGAGTTTAGTGGTGGTTTAAAGCAGCCTCTGACTTCAACCGAGAGAGAGTTTAGTGGTGGTTTAAAGCAGCCTCTTTCGGTTCCTCAGCCGCCGCCAATGCCTCCTTCACTGAGACTCTGGGAGAAACCAAGTCCTAGAACACCAGCTGGGCAAGTGTTCTCTAGGCCTCCAGCTCTCATACCTCCTTCAAGACCTTTTGTGCTTCAAAACCCGGGAAAGGTCTCTGTTTCGCCGGTGAAGTTGACGCCAAGTTCAACACCATTGGAGCCGATTGAGGAGACTCCGAAACCAAAGTTGAAGCCATTGCATTGGGATAAAGTTAGAGCAAGCTCCGATCGCGAAATGGTGTGGGATCAGTTTAGATCGAGCTCTTTCAAATTGAATGAAGAAATGATAGAGACATTGTTTGTTGTAAAAACACCAAACCCGAATCCAAGGGAAACGACCCCACGTGCAGTTCTTCCCTCACCAAATCAAGAGAATAGAGTACTTGATCCAAAAAAGTCGCAGAATATTGCAATCTCACTAAGGGCTCTCAATGTGACCATAGAGGAAGTTTGTGAAGCTTTATTAGAAGGAAACTCGGACGCTCTTGGAACTGAGTTACTTGAAAGTCTAATGAAGATGGCTCCCACCAAAGAAGAAGAACGGAAGCTGAAGGAATACAAAGACAACTCACCAGTTAACCTTGGTCCTGCTGAGAGATTTCTGAAAGAGCTGCTTGACGTTCCTTTTGCATTTAAAAGGATTGATGCAATGCTTTACATGACTAATTTTGAGTCTGAGATCGAGTACCTTAAAAAGTCGTTTGAAACTTTGGAGGCTGCCTGTGAAGAATTGAGAAACAGCAGGATGTTCTTGAAGCTTCTGGAAGCTGTTCTGAAGACTGGAAACCGGATGAATGTTGGGACAAACCGTGGTGATGCCCAAGCCTTCAAACTGGACACACTCCTCAAGCTGGTTGACGTCAAGGGGGCAGATGGGAAAACCACACTCTTGCATTTTGTCGTACAAGAAATCATAAGGACGGAAGGTGCTCGTCTCAACGGTGGCAATCAAACTTCAAACCCAACCTTGAGTGATGATGTCAAGTGCAGGAGGCTTGGCCTGCAAGTTGTTTCAGCTCTCAGTTCAGACCTCAGTAACGTGAAGAAAGCTGCTGTCATGGATTCTGATGTGCTGAGCAGTGATGTTTCCAAGCTTTCTAAAGGAATTAGCAACATCGCGGAGGTTGTGCAATTAAATGAGACGACGGTGTCAGATGACAGCAGCCGGAAATTCTCAGAGTCGATGAACATGTTCATGAAATTGGCTGAAGAGGAGATTATAAGACTTCAAGCTCAAGAAAGTGTTGCATTGTCCCTGGTGAAGGAGATTACAGAGTACTTCCACGGGAACTCCGCTAGGGAAGAAGCTCACCCGTTTAGAATCTTCATGGTGGTCAGAGACTTTCTTACGGTTCTTGATCGGGTATGTAAGGAAGTTGGTTCAATAAATGAGCGAACCCAAGTTATTACCGCCCACAAATTTCCAGTTCCAGTAAATCCAATGCTACCACAAGTAGTTCCAGTAAATCCAATGCTGCCACAACTACTTCCTGGAATTCATGGGTTGAGGCGGTACAGTTCATCCGATGATGAGAGCGCGTCTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005623 GO:0005886 GO:0006996 GO:0007010 GO:0007015 GO:0008064 GO:0008092 GO:0008150 GO:0009987 GO:0010638 GO:0010639 GO:0016020 GO:0016043 GO:0030029 GO:0030036 GO:0030312 GO:0030832 GO:0030833 GO:0030834 GO:0030835 GO:0030837 GO:0030838 GO:0031333 GO:0031334 GO:0032271 GO:0032272 GO:0032273 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0043242 GO:0043244 GO:0043254 GO:0044087 GO:0044089 GO:0044464 GO:0044877 GO:0045010 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050794 GO:0051015 GO:0051016 GO:0051128 GO:0051129 GO:0051130 GO:0051493 GO:0051494 GO:0051495 GO:0051693 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0097435 GO:0110053 GO:1901879 GO:1901880 GO:1902903 GO:1902904 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

1116

Amino Acids

122.18

Weight (kDa)

6.46

Isoelectric Point (pI)

71.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	657 - 1052	4.3e-121	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007674)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G25500
fragaria_vesca	FvH4_2g17360 FvH4_2g17360 FvH4_2g17360
malus_domestica	MD05G1152500.v1.1 MD10G1147000.v1.1
prunus_persica	Prupe.8G185400_v2.0.a1
pyrus_communis	pycom05g14690 pycom10g12670
rosa_chinensis	RchiOBHm_Chr6g0281991
rosa_laevigata	RLG00000012921
rosa_multiflora	Rmu_sc0000569.1_g000001 Rmu_sc0001255.1_g000001 Rmu_sc0011823.1_g000001
rosa_roxburghii	Rroxscaffold_7G00186650
rosa_rugosa	Rorug06G0144700
rosa_samantha	Rh4CG162200 Rh4CG270300 Rh6AG257900 Rh6BG260900 Rh6CG259800 Rh6DG252200 Rh7AG416400
rosa_wichuraiana	Rw6G022300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	3020
AasI	GACNNNNNNGTC	2 cut(s)	1090, 2947
AatII	GACGTC	2 cut(s)	908, 2661
AbsI	CCTCGAGG	1 cut(s)	703
AccB1I	GGYRCC	2 cut(s)	487, 1045
AccI	GTMKAC	1 cut(s)	657
AccII	CGCG	8 cut(s)	586, 613, 615, 816, 860, 2031, 2918, 3338
AccIII	TCCGGA	1 cut(s)	1234
AclWI	GGATC	9 cut(s)	453, 831, 844, 1245, 1572, 1580, 1593, 2052, 2180
AcoI	YGGCCR	1 cut(s)	3
AcsI	RAATTY	7 cut(s)	1351, 1383, 1548, 1633, 2969, 3218, 3294
AcuI	CTGAAG	4 cut(s)	493, 2369, 2594, 3027
AcvI	CACGTG	1 cut(s)	2144
AcyI	GRCGYC	4 cut(s)	905, 1046, 1937, 2658
AfaI	GTAC	8 cut(s)	517, 1068, 1212, 2181, 2486, 2703, 3077, 3314
AfiI	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1909, 2650, 3061
AflIII	ACRYGT	1 cut(s)	2988
AgeI	ACCGGT	1 cut(s)	733
AhdI	GACNNNNNGTC	1 cut(s)	445
AjnI	CCWGG	4 cut(s)	564, 845, 3054, 3289
AjuI	GAANNNNNNNTTGG	2 cut(s)	1780, 1812
AloI	GAACNNNNNNTCC	4 cut(s)	767, 799, 1397, 1429
Alw21I	GWGCWC	6 cut(s)	354, 468, 920, 1532, 2063, 2731
AlwI	GGATC	9 cut(s)	453, 831, 844, 1245, 1572, 1580, 1593, 2052, 2180
AlwNI	CAGNNNCTG	2 cut(s)	1675, 1720
Ama87I	CYCGRG	3 cut(s)	593, 703, 1903
Aor13HI	TCCGGA	1 cut(s)	1234
AoxI	GGCC	6 cut(s)	3, 62, 633, 1389, 1856, 2797
ApoI	RAATTY	7 cut(s)	1351, 1383, 1548, 1633, 2969, 3218, 3294
ArsI	GACNNNNNNTTYG	6 cut(s)	863, 895, 895, 927, 2408, 2440
AsiGI	ACCGGT	1 cut(s)	733
AsiSI	GCGATCGC	1 cut(s)	814
Asp700I	GAANNNNTTC	3 cut(s)	735, 2568, 2990
AspLEI	GCGC	5 cut(s)	317, 588, 615, 1048, 3338
AspS9I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2384
AsuC2I	CCSGG	4 cut(s)	428, 841, 1904, 1905
AvaI	CYCGRG	3 cut(s)	593, 703, 1903
AvaII	GGWCC	5 cut(s)	526, 667, 843, 1135, 2384
BaeGI	GKGCMC	2 cut(s)	492, 572
BalI	TGGCCA	1 cut(s)	5
BamHI	GGATCC	2 cut(s)	836, 1585
BanI	GGYRCC	2 cut(s)	487, 1045
BanII	GRGCYC	4 cut(s)	1011, 1532, 2063, 2227
BbrPI	CACGTG	1 cut(s)	2144
BbsI	GAAGAC	6 cut(s)	795, 915, 1086, 1205, 1614, 2582
Bbv12I	GWGCWC	6 cut(s)	354, 468, 920, 1532, 2063, 2731
BbvCI	CCTCAGC	2 cut(s)	1027, 1776
BccI	CCATC	6 cut(s)	127, 214, 1132, 1432, 2311, 2666
BceAI	ACGGC	8 cut(s)	332, 344, 359, 699, 889, 1033, 1059, 1060
BciT130I	CCWGG	4 cut(s)	566, 847, 3056, 3291
BclI	TGATCA	1 cut(s)	1318
BcnI	CCSGG	4 cut(s)	428, 841, 1904, 1905
BfaI	CTAG	3 cut(s)	1827, 1854, 3096
BfoI	RGCGCY	2 cut(s)	318, 1049
BlpI	GCTNAGC	3 cut(s)	710, 1531, 2873
BmcAI	AGTACT	2 cut(s)	2181, 3077
Bme1390I	CCNGG	8 cut(s)	428, 566, 841, 847, 1904, 1905, 3056, 3291
Bme18I	GGWCC	5 cut(s)	526, 667, 843, 1135, 2384
BmeRI	GACNNNNNGTC	1 cut(s)	445
BmeT110I	CYCGRG	3 cut(s)	593, 703, 1903
BmgT120I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2384
BmiI	GGNNCC	8 cut(s)	489, 838, 844, 1047, 1587, 1773, 1959, 2322
BmrFI	CCNGG	8 cut(s)	428, 566, 841, 847, 1904, 1905, 3056, 3291
BmrI	ACTGGG	1 cut(s)	155
BmsI	GCATC	6 cut(s)	62, 1139, 1301, 1360, 2434, 2605
BmuI	ACTGGG	1 cut(s)	155
BpiI	GAAGAC	6 cut(s)	795, 915, 1086, 1205, 1614, 2582
BplI	GAGNNNNNCTC	5 cut(s)	773, 805, 1514, 1546, 3325
BpmI	CTGGAG	3 cut(s)	295, 656, 1845
Bpu10I	CCTNAGC	2 cut(s)	1027, 1776
Bpu1102I	GCTNAGC	3 cut(s)	710, 1531, 2873
BpuEI	CTTGAG	3 cut(s)	2630, 2788, 3018
BpuMI	CCSGG	4 cut(s)	428, 841, 1904, 1905
BsaAI	YACGTR	1 cut(s)	2144
BsaHI	GRCGYC	4 cut(s)	905, 1046, 1937, 2658
BsaI	GGTCTC	2 cut(s)	1155, 1918
BsaJI	CCNNGG	8 cut(s)	59, 565, 1903, 2126, 2380, 2608, 3054, 3082
BsaWI	WCCGGW	7 cut(s)	733, 973, 1180, 1234, 1261, 1639, 2586
Bsc4I	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1909, 2650, 3061
Bse118I	RCCGGY	4 cut(s)	533, 733, 1213, 1924
Bse1I	ACTGG	9 cut(s)	161, 168, 1347, 2372, 2584, 2637, 3224, 3230, 3260
Bse3DI	GCAATG	3 cut(s)	285, 1994, 2454
BseAI	TCCGGA	1 cut(s)	1234
BseBI	CCWGG	4 cut(s)	566, 847, 3056, 3291
BseDI	CCNNGG	8 cut(s)	59, 565, 1903, 2126, 2380, 2608, 3054, 3082
BseGI	GGATG	4 cut(s)	206, 2550, 2595, 3320
BseLI	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1909, 2650, 3061
BseMI	GCAATG	3 cut(s)	285, 1994, 2454
BseNI	ACTGG	9 cut(s)	161, 168, 1347, 2372, 2584, 2637, 3224, 3230, 3260
BseRI	GAGGAG	4 cut(s)	268, 1982, 2633, 3026
BseSI	GKGCMC	2 cut(s)	492, 572
BseYI	CCCAGC	2 cut(s)	294, 1838
BsgI	GTGCAG	2 cut(s)	2166, 2806
Bsh1236I	CGCG	8 cut(s)	586, 613, 615, 816, 860, 2031, 2918, 3338
Bsh1285I	CGRYCG	4 cut(s)	814, 1073, 1230, 2029
BshFI	GGCC	6 cut(s)	5, 64, 635, 1391, 1858, 2799
BshNI	GGYRCC	2 cut(s)	487, 1045
BshTI	ACCGGT	1 cut(s)	733
BsiEI	CGRYCG	4 cut(s)	814, 1073, 1230, 2029
BsiHKAI	GWGCWC	6 cut(s)	354, 468, 920, 1532, 2063, 2731
BsiHKCI	CYCGRG	3 cut(s)	593, 703, 1903
BslFI	GGGAC	3 cut(s)	972, 2614, 3037
BslI	CCNNNNNNNGG	7 cut(s)	565, 846, 1187, 1514, 1909, 2650, 3061
BsmBI	CGTCTC	3 cut(s)	948, 2738, 2933
BsmFI	GGGAC	3 cut(s)	972, 2614, 3037
BsmI	GAATGC	2 cut(s)	383, 730
BsnI	GGCC	6 cut(s)	5, 64, 635, 1391, 1858, 2799
Bso31I	GGTCTC	2 cut(s)	1155, 1918
BsoBI	CYCGRG	3 cut(s)	593, 703, 1903
Bsp13I	TCCGGA	1 cut(s)	1234
Bsp1720I	GCTNAGC	3 cut(s)	710, 1531, 2873
Bsp68I	TCGCGA	2 cut(s)	816, 2031
BspANI	GGCC	6 cut(s)	5, 64, 635, 1391, 1858, 2799
BspEI	TCCGGA	1 cut(s)	1234
BspFNI	CGCG	8 cut(s)	586, 613, 615, 816, 860, 2031, 2918, 3338
BspHI	TCATGA	1 cut(s)	2994
BspLI	GGNNCC	8 cut(s)	489, 838, 844, 1047, 1587, 1773, 1959, 2322
BspPI	GGATC	9 cut(s)	453, 831, 844, 1245, 1572, 1580, 1593, 2052, 2180
BspQI	GCTCTTC	3 cut(s)	35, 999, 1520
BspT107I	GGYRCC	2 cut(s)	487, 1045
BspTNI	GGTCTC	2 cut(s)	1155, 1918
BsrDI	GCAATG	3 cut(s)	285, 1994, 2454
BsrFI	RCCGGY	4 cut(s)	533, 733, 1213, 1924
BsrI	ACTGG	9 cut(s)	161, 168, 1347, 2372, 2584, 2637, 3224, 3230, 3260
BssAI	RCCGGY	4 cut(s)	533, 733, 1213, 1924
BssECI	CCNNGG	8 cut(s)	59, 565, 1903, 2126, 2380, 2608, 3054, 3082
BssNI	GRCGYC	4 cut(s)	905, 1046, 1937, 2658
BssT1I	CCWWGG	3 cut(s)	59, 2126, 2380
Bst2UI	CCWGG	4 cut(s)	566, 847, 3056, 3291
Bst6I	CTCTTC	7 cut(s)	35, 759, 765, 774, 999, 1520, 3003
BstACI	GRCGYC	4 cut(s)	905, 1046, 1937, 2658
BstBAI	YACGTR	1 cut(s)	2144
BstC8I	GCNNGC	3 cut(s)	476, 2020, 2801
BstDSI	CCRYGG	2 cut(s)	2608, 3082
BstENI	CCTNNNNNAGG	1 cut(s)	3059
BstF5I	GGATG	4 cut(s)	206, 2550, 2595, 3320
BstFNI	CGCG	8 cut(s)	586, 613, 615, 816, 860, 2031, 2918, 3338
BstH2I	RGCGCY	2 cut(s)	318, 1049
BstHHI	GCGC	5 cut(s)	317, 588, 615, 1048, 3338
BstMCI	CGRYCG	4 cut(s)	814, 1073, 1230, 2029
BstNI	CCWGG	4 cut(s)	566, 847, 3056, 3291
BstNSI	RCATGY	2 cut(s)	1292, 2992
BstSCI	CCNGG	8 cut(s)	426, 564, 839, 845, 1902, 1903, 3054, 3289
BstSLI	GKGCMC	2 cut(s)	492, 572
BstUI	CGCG	8 cut(s)	586, 613, 615, 816, 860, 2031, 2918, 3338
BstV2I	GAAGAC	6 cut(s)	795, 915, 1086, 1205, 1614, 2582
BstX2I	RGATCY	3 cut(s)	458, 836, 1585
BstXI	CCANNNNNNTGG	1 cut(s)	2001
BstYI	RGATCY	3 cut(s)	458, 836, 1585
BsuRI	GGCC	6 cut(s)	5, 64, 635, 1391, 1858, 2799
BtgI	CCRYGG	2 cut(s)	2608, 3082
BtgZI	GCGATG	1 cut(s)	2899
BtsCI	GGATG	4 cut(s)	206, 2550, 2595, 3320
BtsI	GCAGTG	2 cut(s)	246, 2884
BtsIMutI	CAGTG	7 cut(s)	246, 517, 579, 1340, 1508, 1799, 2884
BtuMI	TCGCGA	2 cut(s)	816, 2031
Cac8I	GCNNGC	3 cut(s)	476, 2020, 2801
CaiI	CAGNNNCTG	2 cut(s)	1675, 1720
CciI	TCATGA	1 cut(s)	2994
CfoI	GCGC	5 cut(s)	317, 588, 615, 1048, 3338
Cfr10I	RCCGGY	4 cut(s)	533, 733, 1213, 1924
Cfr13I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2384
Cfr9I	CCCGGG	1 cut(s)	1903
CpoI	CGGWCCG	1 cut(s)	1135
CseI	GACGC	3 cut(s)	1945, 2288, 3327
Csp6I	GTAC	8 cut(s)	516, 1067, 1211, 2180, 2485, 2702, 3076, 3313
CspAI	ACCGGT	1 cut(s)	733
CspI	CGGWCCG	1 cut(s)	1135
CviQI	GTAC	8 cut(s)	516, 1067, 1211, 2180, 2485, 2702, 3076, 3313
DinI	GGCGCC	1 cut(s)	1047
DraI	TTTAAA	4 cut(s)	1665, 1710, 1755, 2434
DrdI	GACNNNNNNGTC	2 cut(s)	1090, 2947
DriI	GACNNNNNGTC	1 cut(s)	445
DseDI	GACNNNNNNGTC	2 cut(s)	1090, 2947
EaeI	YGGCCR	1 cut(s)	3
Eam1104I	CTCTTC	7 cut(s)	35, 759, 765, 774, 999, 1520, 3003
Eam1105I	GACNNNNNGTC	1 cut(s)	445
EarI	CTCTTC	7 cut(s)	35, 759, 765, 774, 999, 1520, 3003
EciI	GGCGGA	3 cut(s)	213, 346, 672
Ecl136II	GAGCTC	2 cut(s)	1530, 2061
Eco130I	CCWWGG	3 cut(s)	59, 2126, 2380
Eco147I	AGGCCT	1 cut(s)	1858
Eco24I	GRGCYC	4 cut(s)	1011, 1532, 2063, 2227
Eco31I	GGTCTC	2 cut(s)	1155, 1918
Eco32I	GATATC	1 cut(s)	1170
Eco47I	GGWCC	5 cut(s)	526, 667, 843, 1135, 2384
Eco53kI	GAGCTC	2 cut(s)	1530, 2061
Eco57I	CTGAAG	4 cut(s)	493, 2369, 2594, 3027
Eco72I	CACGTG	1 cut(s)	2144
Eco88I	CYCGRG	3 cut(s)	593, 703, 1903
EcoICRI	GAGCTC	2 cut(s)	1530, 2061
EcoNI	CCTNNNNNAGG	1 cut(s)	3059
EcoRI	GAATTC	2 cut(s)	1633, 3294
EcoRII	CCWGG	4 cut(s)	564, 845, 3054, 3289
EcoRV	GATATC	1 cut(s)	1170
EcoT14I	CCWWGG	3 cut(s)	59, 2126, 2380
EcoT22I	ATGCAT	2 cut(s)	1038, 1294
EcoT38I	GRGCYC	4 cut(s)	1011, 1532, 2063, 2227
EgeI	GGCGCC	1 cut(s)	1047
EheI	GGCGCC	1 cut(s)	1047
ErhI	CCWWGG	3 cut(s)	59, 2126, 2380
Esp3I	CGTCTC	3 cut(s)	948, 2738, 2933
FalI	AAGNNNNNCTT	2 cut(s)	2397, 2429
FaqI	GGGAC	3 cut(s)	972, 2614, 3037
FauI	CCCGC	3 cut(s)	274, 608, 618
FbaI	TGATCA	1 cut(s)	1318
FblI	GTMKAC	1 cut(s)	657
FokI	GGATG	4 cut(s)	193, 2557, 2602, 3307
FriOI	GRGCYC	4 cut(s)	1011, 1532, 2063, 2227
FspBI	CTAG	3 cut(s)	1827, 1854, 3096
GlaI	GCGC	5 cut(s)	316, 587, 614, 1047, 3337
GsaI	CCCAGC	2 cut(s)	298, 1842
GsuI	CTGGAG	3 cut(s)	295, 656, 1845
HaeII	RGCGCY	2 cut(s)	318, 1049
HaeIII	GGCC	6 cut(s)	5, 64, 635, 1391, 1858, 2799
HgaI	GACGC	3 cut(s)	1945, 2288, 3327
HhaI	GCGC	5 cut(s)	317, 588, 615, 1048, 3338
Hin1I	GRCGYC	4 cut(s)	905, 1046, 1937, 2658
Hin6I	GCGC	5 cut(s)	315, 586, 613, 1046, 3336
HinP1I	GCGC	5 cut(s)	315, 586, 613, 1046, 3336
HincII	GTYRAC	7 cut(s)	580, 607, 658, 1083, 1935, 2377, 2656
HindII	GTYRAC	7 cut(s)	580, 607, 658, 1083, 1935, 2377, 2656
HindIII	AAGCTT	3 cut(s)	2256, 2555, 2891
HpaI	GTTAAC	1 cut(s)	2377
Hpy166II	GTNNAC	9 cut(s)	518, 580, 607, 658, 938, 1083, 1935, 2377, 2656
Hpy8I	GTNNAC	9 cut(s)	518, 580, 607, 658, 938, 1083, 1935, 2377, 2656
Hpy99I	CGWCG	7 cut(s)	380, 402, 907, 910, 1081, 1159, 2947
HpyCH4IV	ACGT	5 cut(s)	905, 2143, 2418, 2658, 2838
HpySE526I	ACGT	5 cut(s)	905, 2143, 2418, 2658, 2838
Hsp92I	GRCGYC	4 cut(s)	905, 1046, 1937, 2658
HspAI	GCGC	5 cut(s)	315, 586, 613, 1046, 3336
KasI	GGCGCC	1 cut(s)	1045
Kpn2I	TCCGGA	1 cut(s)	1234
Ksp22I	TGATCA	1 cut(s)	1318
KspAI	GTTAAC	1 cut(s)	2377
LguI	GCTCTTC	3 cut(s)	35, 999, 1520
LmnI	GCTCC	6 cut(s)	554, 618, 827, 1957, 2027, 2326
LweI	GCATC	6 cut(s)	62, 1139, 1301, 1360, 2434, 2605
MaeI	CTAG	3 cut(s)	1827, 1854, 3096
MaeII	ACGT	5 cut(s)	905, 2143, 2418, 2658, 2838
MaeIII	GTNAC	8 cut(s)	948, 1176, 1374, 1443, 1503, 2233, 2295, 2834
MflI	RGATCY	3 cut(s)	458, 836, 1585
MlsI	TGGCCA	1 cut(s)	5
MluNI	TGGCCA	1 cut(s)	5
Mly113I	GGCGCC	1 cut(s)	1046
MlyI	GAGTC	8 cut(s)	84, 585, 653, 1078, 1800, 1966, 2480, 2987
MmeI	TCCRAC	1 cut(s)	198
Mox20I	TGGCCA	1 cut(s)	5
Mph1103I	ATGCAT	2 cut(s)	1038, 1294
MroI	TCCGGA	1 cut(s)	1234
MroXI	GAANNNNTTC	3 cut(s)	735, 2568, 2990
MscI	TGGCCA	1 cut(s)	5
MseI	TTAA	9 cut(s)	983, 1349, 1664, 1709, 1754, 2376, 2433, 2490, 2933
MslI	CAYNNNNRTG	1 cut(s)	2993
Msp20I	TGGCCA	1 cut(s)	5
MspA1I	CMGCKG	2 cut(s)	1535, 1838
MspR9I	CCNGG	8 cut(s)	428, 566, 841, 847, 1904, 1905, 3056, 3291
Mva1269I	GAATGC	2 cut(s)	383, 730
MvaI	CCWGG	4 cut(s)	566, 847, 3056, 3291
MvnI	CGCG	8 cut(s)	586, 613, 615, 816, 860, 2031, 2918, 3338
NarI	GGCGCC	1 cut(s)	1046
NciI	CCSGG	4 cut(s)	428, 841, 1904, 1905
NlaIV	GGNNCC	8 cut(s)	489, 838, 844, 1047, 1587, 1773, 1959, 2322
NmuCI	GTSAC	5 cut(s)	948, 1176, 1443, 1503, 2233
NruI	TCGCGA	2 cut(s)	816, 2031
NsiI	ATGCAT	2 cut(s)	1038, 1294
NspI	RCATGY	2 cut(s)	1292, 2992
PaeR7I	CTCGAG	2 cut(s)	593, 703
PagI	TCATGA	1 cut(s)	2994
PceI	AGGCCT	1 cut(s)	1858
PciI	ACATGT	1 cut(s)	2988
PciSI	GCTCTTC	3 cut(s)	35, 999, 1520
PcsI	WCGNNNNNNNCGW	3 cut(s)	911, 1163, 1172
PctI	GAATGC	2 cut(s)	383, 730
PdmI	GAANNNNTTC	3 cut(s)	735, 2568, 2990
PfeI	GAWTC	6 cut(s)	104, 1457, 1472, 2122, 2861, 3120
PflFI	GACNNNGTC	1 cut(s)	2947
PfoI	TCCNGGA	3 cut(s)	426, 845, 3289
PinAI	ACCGGT	1 cut(s)	733
Ple19I	CGATCG	2 cut(s)	814, 2029
PleI	GAGTC	8 cut(s)	83, 585, 653, 1078, 1800, 1966, 2479, 2986
PluTI	GGCGCC	1 cut(s)	1049
PmaCI	CACGTG	1 cut(s)	2144
PmlI	CACGTG	1 cut(s)	2144
PpsI	GAGTC	8 cut(s)	83, 585, 653, 1078, 1800, 1966, 2479, 2986
Ppu21I	YACGTR	1 cut(s)	2144
PscI	ACATGT	1 cut(s)	2988
PsiI	TTATAA	1 cut(s)	3020
Psp124BI	GAGCTC	2 cut(s)	1532, 2063
Psp6I	CCWGG	4 cut(s)	564, 845, 3054, 3289
PspCI	CACGTG	1 cut(s)	2144
PspFI	CCCAGC	2 cut(s)	294, 1838
PspGI	CCWGG	4 cut(s)	564, 845, 3054, 3289
PspN4I	GGNNCC	8 cut(s)	489, 838, 844, 1047, 1587, 1773, 1959, 2322
PspPI	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2384
PspXI	VCTCGAGB	2 cut(s)	593, 703
PstNI	CAGNNNCTG	2 cut(s)	1675, 1720
PsuI	RGATCY	3 cut(s)	458, 836, 1585
PsyI	GACNNNGTC	1 cut(s)	2947
PvuI	CGATCG	2 cut(s)	814, 2029
PvuII	CAGCTG	2 cut(s)	1535, 1838
RgaI	GCGATCGC	1 cut(s)	814
RruI	TCGCGA	2 cut(s)	816, 2031
RsaI	GTAC	8 cut(s)	517, 1068, 1212, 2181, 2486, 2703, 3077, 3314
RsaNI	GTAC	8 cut(s)	516, 1067, 1211, 2180, 2485, 2702, 3076, 3313
RseI	CAYNNNNRTG	1 cut(s)	2993
Rsr2I	CGGWCCG	1 cut(s)	1135
RsrII	CGGWCCG	1 cut(s)	1135
SacI	GAGCTC	2 cut(s)	1532, 2063
SalI	GTCGAC	1 cut(s)	656
SapI	GCTCTTC	3 cut(s)	35, 999, 1520
SaqAI	TTAA	9 cut(s)	983, 1349, 1664, 1709, 1754, 2376, 2433, 2490, 2933
Sau96I	GGNCC	6 cut(s)	526, 633, 667, 843, 1135, 2384
ScaI	AGTACT	2 cut(s)	2181, 3077
SchI	GAGTC	8 cut(s)	84, 585, 653, 1078, 1800, 1966, 2480, 2987
ScrFI	CCNGG	8 cut(s)	428, 566, 841, 847, 1904, 1905, 3056, 3291
SfaAI	GCGATCGC	1 cut(s)	814
SfaNI	GCATC	6 cut(s)	62, 1139, 1301, 1360, 2434, 2605
SfoI	GGCGCC	1 cut(s)	1047
Sfr274I	CTCGAG	2 cut(s)	593, 703
SgfI	GCGATCGC	1 cut(s)	814
SgrAI	CRCCGGYG	1 cut(s)	1924
SinI	GGWCC	5 cut(s)	526, 667, 843, 1135, 2384
SlaI	CTCGAG	2 cut(s)	593, 703
SmaI	CCCGGG	1 cut(s)	1905
SmiMI	CAYNNNNRTG	1 cut(s)	2993
SmlI	CTYRAG	5 cut(s)	593, 703, 2645, 2767, 3033
SmoI	CTYRAG	5 cut(s)	593, 703, 2645, 2767, 3033
SseBI	AGGCCT	1 cut(s)	1858
SspDI	GGCGCC	1 cut(s)	1045
SspI	AATATT	1 cut(s)	2206
SspMI	CTAG	3 cut(s)	1827, 1854, 3096
SstI	GAGCTC	2 cut(s)	1532, 2063
StuI	AGGCCT	1 cut(s)	1858
StyD4I	CCNGG	8 cut(s)	426, 564, 839, 845, 1902, 1903, 3054, 3289
StyI	CCWWGG	3 cut(s)	59, 2126, 2380
TaiI	ACGT	5 cut(s)	908, 2146, 2421, 2661, 2841
TaqII	GACCGA	4 cut(s)	765, 936, 1152, 1216
TatI	WGTACW	2 cut(s)	2179, 3075
TfiI	GAWTC	6 cut(s)	104, 1457, 1472, 2122, 2861, 3120
Tru1I	TTAA	9 cut(s)	983, 1349, 1664, 1709, 1754, 2376, 2433, 2490, 2933
Tru9I	TTAA	9 cut(s)	983, 1349, 1664, 1709, 1754, 2376, 2433, 2490, 2933
TscAI	CASTG	7 cut(s)	253, 517, 579, 1347, 1515, 1806, 2884
TseFI	GTSAC	5 cut(s)	948, 1176, 1443, 1503, 2233
Tsp45I	GTSAC	5 cut(s)	948, 1176, 1443, 1503, 2233
TspGWI	ACGGA	5 cut(s)	849, 1143, 1158, 2356, 2735
TspMI	CCCGGG	1 cut(s)	1903
TspRI	CASTG	7 cut(s)	253, 517, 579, 1347, 1515, 1806, 2884
Tth111I	GACNNNGTC	1 cut(s)	2947
VpaK11BI	GGWCC	5 cut(s)	526, 667, 843, 1135, 2384
XagI	CCTNNNNNAGG	1 cut(s)	3059
XapI	RAATTY	7 cut(s)	1351, 1383, 1548, 1633, 2969, 3218, 3294
XceI	RCATGY	2 cut(s)	1292, 2992
XcmI	CCANNNNNNNNNTGG	1 cut(s)	509
XhoI	CTCGAG	2 cut(s)	593, 703
XmaI	CCCGGG	1 cut(s)	1903
XmiI	GTMKAC	1 cut(s)	657
XmnI	GAANNNNTTC	3 cut(s)	735, 2568, 2990
XspI	CTAG	3 cut(s)	1827, 1854, 3096
ZraI	GACGTC	2 cut(s)	906, 2659
ZrmI	AGTACT	2 cut(s)	2181, 3077
Zsp2I	ATGCAT	2 cut(s)	1038, 1294

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.